JP6796932B2 - 分離装置 - Google Patents

分離装置 Download PDF

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Publication number
JP6796932B2
JP6796932B2 JP2016026673A JP2016026673A JP6796932B2 JP 6796932 B2 JP6796932 B2 JP 6796932B2 JP 2016026673 A JP2016026673 A JP 2016026673A JP 2016026673 A JP2016026673 A JP 2016026673A JP 6796932 B2 JP6796932 B2 JP 6796932B2
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electrodes
electrode
flow path
cells
dielectric particles
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JP2017143763A5 (https=
JP2017143763A (ja
Inventor
嘉一 脇坂
嘉一 脇坂
雅代 高野
雅代 高野
隆行 糸井
隆行 糸井
隆治 円城寺
隆治 円城寺
雅和 戸井
雅和 戸井
友美 西村
友美 西村
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AFI CORPORATION
Kyoto University NUC
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AFI CORPORATION
Kyoto University NUC
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Priority to JP2016026673A priority Critical patent/JP6796932B2/ja
Priority to CN201780011565.1A priority patent/CN108699506A/zh
Priority to PCT/JP2017/003301 priority patent/WO2017141685A1/ja
Priority to US15/999,452 priority patent/US20210205822A1/en
Priority to EP17752948.4A priority patent/EP3418373B1/en
Publication of JP2017143763A publication Critical patent/JP2017143763A/ja
Publication of JP2017143763A5 publication Critical patent/JP2017143763A5/ja
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/005—Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0424—Dielectrophoretic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/26—Details of magnetic or electrostatic separation for use in medical or biological applications
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Sustainable Development (AREA)
  • Electrochemistry (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Fluid Mechanics (AREA)
  • Physics & Mathematics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Electrostatic Separation (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2016026673A 2016-02-16 2016-02-16 分離装置 Active JP6796932B2 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2016026673A JP6796932B2 (ja) 2016-02-16 2016-02-16 分離装置
CN201780011565.1A CN108699506A (zh) 2016-02-16 2017-01-31 分离装置
PCT/JP2017/003301 WO2017141685A1 (ja) 2016-02-16 2017-01-31 分離装置
US15/999,452 US20210205822A1 (en) 2016-02-16 2017-01-31 Separation device
EP17752948.4A EP3418373B1 (en) 2016-02-16 2017-01-31 Separation device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2016026673A JP6796932B2 (ja) 2016-02-16 2016-02-16 分離装置

Publications (3)

Publication Number Publication Date
JP2017143763A JP2017143763A (ja) 2017-08-24
JP2017143763A5 JP2017143763A5 (https=) 2019-03-22
JP6796932B2 true JP6796932B2 (ja) 2020-12-09

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JP2016026673A Active JP6796932B2 (ja) 2016-02-16 2016-02-16 分離装置

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US (1) US20210205822A1 (https=)
EP (1) EP3418373B1 (https=)
JP (1) JP6796932B2 (https=)
CN (1) CN108699506A (https=)
WO (1) WO2017141685A1 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110628568B (zh) * 2019-09-30 2021-10-01 北京化工大学 用于高通量连续流细胞分离的滑轨式介电泳电极结构
US11454583B2 (en) * 2019-12-27 2022-09-27 Imec Vzw Field-array free flow fractionation
US12181448B2 (en) 2019-12-27 2024-12-31 Imec Vzw Method for continuously separating components from a sample
CN113717846B (zh) * 2020-05-26 2023-08-29 中国科学院青岛生物能源与过程研究所 一种基于介电确定性位移的细胞分选芯片、装置及方法
EP3919171A1 (en) * 2020-06-05 2021-12-08 Ecole Polytechnique Fédérale de Lausanne (EPFL) Dielectrophoresis detection device
EP4337756A1 (en) * 2021-05-13 2024-03-20 Janssen Biotech, Inc. Device and method for sorting biological cells
EP4201526A1 (en) * 2021-12-22 2023-06-28 Imec VZW Microfluidic device for sorting particles

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6071394A (en) * 1996-09-06 2000-06-06 Nanogen, Inc. Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis
DE59907639D1 (de) * 1998-06-26 2003-12-11 Evotec Ag Elektrodenanordnung zur dielektrophoretischen partikelablenkung
GB9916850D0 (en) * 1999-07-20 1999-09-22 Univ Wales Bangor Dielectrophoretic apparatus & method
KR100745754B1 (ko) * 2005-12-29 2007-08-02 삼성전자주식회사 금속 기둥 전극 구조를 포함하는 유전 영동을 이용하여입자를 조작하기 위한 장치 및 그를 이용하여 빠른유속으로 유전 영동에 의하여 입자를 조작할 수 있는 방법
US8361296B2 (en) * 2007-05-18 2013-01-29 University Of Washington Shaped electrodes for microfluidic dielectrophoretic particle manipulation
WO2012054904A2 (en) * 2010-10-21 2012-04-26 The Regents Of The University Of California Microfluidics with wirelessly powered electronic circuits
JP5617532B2 (ja) * 2010-10-29 2014-11-05 ソニー株式会社 誘電サイトメトリ装置及び誘電サイトメトリによる細胞分取方法
JP5921829B2 (ja) 2011-07-29 2016-05-24 シャープ株式会社 捕集装置、分離方法、および表示方法
WO2013019810A2 (en) * 2011-08-02 2013-02-07 Tokyo Electron Limited Method and device for controlling pattern and structure formation by an electric field
JP6299749B2 (ja) * 2013-03-26 2018-03-28 ソニー株式会社 測定装置

Also Published As

Publication number Publication date
EP3418373B1 (en) 2022-02-23
EP3418373A1 (en) 2018-12-26
EP3418373A4 (en) 2019-08-28
WO2017141685A1 (ja) 2017-08-24
CN108699506A (zh) 2018-10-23
US20210205822A1 (en) 2021-07-08
JP2017143763A (ja) 2017-08-24

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