JP6725955B1 - Zhuang pharmaceutical composition for blood supplementation and method for preparing the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a Zhuang pharmaceutical composition for blood supplementation and purification and a method for preparing the same. The present invention discloses a Zhuang family pharmaceutical composition for blood supplementation, which composition comprises 20 to 60 parts by weight of Koshinomoto, 10 to 50 parts by weight of Futoto, 10 to 60 parts by weight of Scutellaria baicalensis, and 10 to 100 parts of carrot. 50 parts by weight, kiban 10 to 30 parts by weight, longan 10 to 50 parts by weight, density 10 to 40 parts by weight, seisei 10 to 25 parts by weight, senbou 10 to 30 parts by weight, and a preparation method thereof are also used. provide. Advantageous effects of the present invention are as follows. The Zhuang pharmaceutical composition according to the present invention has the effects of supplementing blood, supplementing sperm, assisting sanity, removing innocence, and improving the flow of three spirits, and makes the complexion pale and blemishes. It improves the symptoms of inability to see clearly, palpitation, sleeplessness, white nails, has a positive therapeutic effect, is fast-acting, stable, has no side effects, and provides a method for its preparation. The preparation method is easy to operate, and the efficacy of the crude drug can be sufficiently exerted. [Selection diagram] None

Description

TECHNICAL FIELD The present invention belongs to the field of ethnic medicine, and specifically relates to a Zhuang pharmaceutical composition for blood supplementation and purification, and a method for preparing the same.

Zhuang medicine, which is an important part of ethnic medicine, has been an effective means for the prevention and treatment of diseases in Zhuang since ancient times, and has played an important role in the physical health and development of survival of Zhuang people. According to Zhuang medicine, the human body is an organic whole, whose constituent parts are closely related and indivisible. Human body "Takumi" (top), "Corridor" (human part), "Torso" (ground), three parts, three ways (valley, airway, water supply), both roads (long road, fire road), internal organs , And “deprivation” (bone), “acceptance” (flesh) “lie” (ki), “bule” (blood), etc. are all related to each other, interact with each other, function in synchronization with the natural world, and are constrained. It also undergoes metaplasia, and changes life and death repeatedly. If your body isn't sane enough,
Poisons, scorpions, poisons, and other toxic invades through the dragon's way and fire's way, causing poor flow in the water supply, valleys, and respiratory tracts, imbalance in visceral bone and flesh, or insufficient nutrition. , If the three spirits of the heavenly land are broken together, various diseases are more likely to occur. For the treatment of diseases, Zhuang medicine emphasizes investigating the etiology, mainly focuses on diagnosis of diseases, and often applies appropriate prescriptions and appropriate herbal medicines. Is one of the main features of. Zhuang medicine has accumulated a wealth of experience in treating diseases, and some methods unique to Zhuang medicine, the prescription of Zhuang medicine, have achieved clinically good results.

According to Zhuang medicine, "bule" (blood) is a very important substance that provides nutrients to the bones and organs of the whole body and the 100 limbs, and it is born from the spirit of heaven and earth Continued splenic stomach weakness, resistance to venom, and inadequate meals cause "Burenai" (blood deficiency), and "Bureuchi" (blood deficiency) has a pale complexion I can't see things clearly, my heart palpitates, I can't sleep, my nails appear white, and so on.Therefore, I supplement my blood, supplement my spirits, help my sanity, get rid of nausea, and improve the flow of three spirits. To develop a Zhuang pharmaceutical composition for blood-feeding and sperm injection, which has the effect of straightening, has a pale complexion, cannot clearly see things with dark eyes, has palpitation, cannot sleep, and has white nails. Is very important.

The technical problem to be solved by the present invention is to supplement blood, replenish spirit, assist sanity, remove innocence, improve the flow of three spirits, prepare a complexion, have a pale complexion, and have chasm eyes. It is an object of the present invention to provide a Zhuang family pharmaceutical composition for blood supplementation and fertilization, which improves symptoms such as inability to see clearly, palpitation, sleeplessness, and white nails, and a method for preparing the same.

The present invention solves the above technical problems by the following technical solutions.

It is a Zhuang family pharmaceutical composition for blood supplementation and fertilization, wherein the composition is 20 to 60 parts by weight of Koshimato, 10 to 50 parts by weight, 10 to 60 parts by weight of kelp nut, 10 to 50 parts by weight of ginseng, and kiban 10 to 30. It is prepared using 10 parts by weight of longan, 10 to 50 parts by weight of longan, 10 to 40 parts by weight of density, 10 to 25 parts by weight of seisei, and 10 to 30 parts by weight of senbou.

Further, in the above-mentioned pharmaceutical composition for blood purification and fertilization, the composition comprises 50 parts by weight of Koshimato, 40 parts by weight of swordfish, 20 parts by weight of radix, 30 parts by weight of ginseng, 30 parts by weight of longan, 20 parts by weight of longan, denshichi It is prepared by using 20 parts by weight, 20 parts by weight of seisei, and 20 parts by weight of senbou.

A method for preparing the above-mentioned blood purification/fertilizing Zhuang family pharmaceutical composition, which comprises washing and selecting raw materials to be weighed in parts by weight, mixing, adding water, and soaking for 0.5 to 1 hour. , After decocting on high heat until the liquid medicine boils, switch to low heat and decoct for 20 to 25 minutes, and then carry out the primary decoction step to filter the medicinal juice and water at room temperature 0.5-1 cm above the plane of the crude drug. Add it again, decoct on high heat until the chemical solution boils, switch to low heat and decoct for 15 to 20 minutes, combine the secondary decoction step of filtering the chemical juice with the chemical juice obtained from the primary and secondary decoctions. And steps. Further, in the above method for preparing a Zhuang pharmaceutical composition for blood supplementation and purification, the dosage form of the Zhuang pharmaceutical composition is a decoction.

A second object of the present invention is to provide a method for preparing the above-mentioned Zhuang family pharmaceutical composition for blood supplementation and fertilization, wherein each raw material to be weighed in parts by weight is washed and selected, and then the crude drug is treated with water. Add 8 times and decoct 3 times per hour, filter and concentrate, dry under reduced pressure at 80°C, pulverize and sieve through a 100 mesh screen, add sucrose to dry paste powder, mix evenly, 75 % Ethanol to make a soft material (ethanol usage 8%), stir for 15 min, granulate with a 12 mesh sieve, dry at 75°C for 4 h, sieving with a 12 mesh sieve and homogenize with a mixer Mix, pack and package.

The characteristics of the raw materials contained in the Zhuang medicine and the Chinese medicine composition according to the present invention are as follows.

Koshimoto: The drug name of the Zhuang family is Gocijcwz, which is derived from the dried roots of the mulberry plant Java gum Ficus hirta Vahl. Taste: sweet, flat. Efficacy: Increases the function of the spleen, strengthens the lungs, improves the flow of air, expels moisture, and smoothes the water supply. Main cure: Splenic edema, anorexia and incompetence, cough due to pulmonary tuberculosis, night sweats, swallowing, postpartum milklessness, irregular menstruation, wind eczema, edema, etc.

Futoto: The name of the Zhuang family medicine is Gaeundaux, and Euonymus fortuei (Turcz.) Hand. -Mazz. Derived from the stalk with leaves. Taste: Bitter, sweet, slightly spicy, fever. Efficacy: Ryuji, arranging fire route to remove the poison. Main cure: Lumbago labor, wind and wet bone pain, blood loss, vaginal bleeding, irregular menstruation, fracture, wound bleeding, etc.

Chinese herb: The Zhuang family medicine name is Goragdingh, which is derived from the roots of the mulberry plant, the sunflower Femingia Philippinensis. Taste: sweet, astringent, flat. Efficacy: Organizes fire routes to eliminate wind and moisture poisons and strengthens muscles and bones. Curative: Wind and bone pain, low back pain, weak limbs, bruise, sore throat.

Ginseng: The drug name of Zhuang family is Gocaengnaengh, which is derived from the root of the plant of the purslane family Talinum paniculatum (Jacq.) Gaertn. Taste: sweet, flat. Efficacy: Improves the condition of the void by improving the flow of airways and valleys and replenishing the air. Chief: qi, dysphoria, dizziness, cough, self-sweat, night sweats, irregular menstruation, obishita, etc.

Kiban: The Zhuang medicine name is Bajbyaj. And is derived from the shell of the turtle family Turtle Chainernys reevesii (Gray). Taste: Ham, sweet, cold. Efficacy: Improve the flow of Ryuji, improve the state of imagination and strengthen bones. Chief: Self-sweat, night sweats, dizziness, weak limbs, amnesia.

Longan has a Zhuang medicine name, Nohmaknganx, and is derived from the pseudoseed of the plant of the sectaceae Longan Dimocarpus longan Lour. The taste is sweet and heat. Efficacy is a fire tract remedy, a god of stability. The main cures are dizziness, sleeplessness, blood loss, collapse, and irregular menstruation.

Density: The name of the Zhuang family medicine is Godienzcaet, and it is Panax notoginseng (Burk) F. H. Chen ex C.I. Derived from the roots of Chow. Taste: sweet, heat. Efficacy: Ryuji, fire regulation, blood supply, hemostasis, decongestion and pain relief. Curative: Postpartum blood loss, blood loss, bloodshed, blood loss, fecal blood, cramps, chest pain, stomach pain, bruise, menstrual pain, postpartum abdominal pain.

Sei: The name of the Zhuang medicine is Ginghsw, which is derived from the rhizome of Polygonatum cyrtonema Hua, which is a flowering plant of the lily family. Taste: sweet, flat. Efficacy: Remove wind poison, improve the valley, improve the function of the spleen and make you feel better. Chief: Cough, splenic fatigue, loss of appetite, dry mouth, dryness, weakness of limbs, dysphorus, tinnitus darkness, whitening of beard hair, thinning body, and scabies.

Ambassador: The Zhuang medicine name is Gohazsien, which is derived from the rhizome of the moss family plant Curcumigo orchioides Gaertn. Taste: Spicy, fever, toxic. Efficacy: Clean the water supply and eliminate the heat and heat poison. Main cure: Yang wilt, urinary incontinence, stomach pain, low back pain, myalgia.

In the prescription, Koshimotou, Futotou, Sengokuto are the main drugs, Donnin and Kiban are the side drugs, Ryugan, Densichi, and Oisei are the public drugs, Senboku is the mother drug, and the whole prescription is the main and sub-parents. Used in combination, it supplements blood, replenishes spirits, assists sanity, removes nausea, and improves the flow of three spirits.

The present invention has the following remarkable effects.

INDUSTRIAL APPLICABILITY The present invention has clear effects of supplementing blood, replenishing spirit, assisting sanity, removing evil, and improving the flow of three spirits, pale complexion, clearly visible things with dark eyes It is a Zhuang pharmaceutical composition for blood supplementation and fertilization that improves symptoms such as palpitation, sleeplessness, and white nails, and provides a preparation method thereof, which is easy to operate and has the efficacy of a crude drug. It can be fully demonstrated.

Hereinafter, the present invention will be further described with reference to examples, but the scope of protection of the present invention is not limited thereto.

1.Preparation process of Zhuang pharmaceutical composition for blood supplementation (1) Design of production process This formulation is based on clinical experience, and generally uses decoction as the dosage form, and the manufacturing method is simple. Yes, it has the advantage that it is not limited by the conditions of the device. However, most of the decoctions are brewed as a single agent, and it is difficult to unify the quality and mass-produce them, difficult to store, inconvenient to carry and transport, and inconvenient to use. In order to solve the above-mentioned drawbacks and make it easier to be received by the majority of patients, granules, which is a dosage form suitable for general administration routes and methods, is used as a preparation dosage form of the product. Water is often used as the extraction solvent for traditional Chinese herbal decoctions, and water extraction is planned to be water because the cost is low and the drug effect is certain. For this reason, the method of the production process is to decoct with water to obtain a dry paste, then add excipients and granulate.

(2) Screening of water extraction process conditions 1. Design of orthogonal test The factors that influence the water extraction effect are (1) number of decoctions, (2) decoction time, (3) water addition amount, (4) crushing degree, Is. In three levels, (see details in Table 1, Table 2) for these factors, and performing an optimization of the orthogonal test, L 9 ₍₃ ⁴⁾ was performed orthogonal test using an orthogonal table.

2. Optimization index and total scoring method There are a total of six herbal medicines that need to be extracted in the formulation of this product, and among them, Kimi-koto is a citrus cortex, which mainly contains flavonoid components, and its representative The main ingredient is psoralen, and the psoralen content level is closely related to the effectiveness of this product and can be an index for optimizing the process conditions of this product. The maximum psoralen content is 60 points. Calculate the relative score of each sample, the total solid content can reflect the extraction efficiency as a whole, as the second optimization index of the process condition of this product, the maximum total solid content as 40 points The relative score of each sample was calculated, and the two scores were added to give the total score of each sample.

3. Optimization method 3.1 Orthogonal test The test is set according to the orthogonal design test table shown in Table 2, and in the test of each group, the prescribed amount of crude drug is prepared, and the decoctions of the respective test numbers are respectively combined and uniformly. After mixing, filtering, and concentrating the filtrate, the volume was adjusted to 1000 ml to obtain a test solution.

3.2 Measurement method Measurement of total solid content 100 ml of the test solution is precisely weighed, placed in an evaporation dish that has been dried to a certain weight at 105°C, steam-dried in a water bath, and dried under reduced pressure to a certain weight at 80°C, and then weighed. Then, the amount of dry paste was calculated.

Measurement of Psoralen Content 5.09 mg of psoralen was precisely weighed, methanol was added and dissolved in a 10 ml volumetric flask to obtain a standard solution. 10 ml of the sample was precisely sucked, placed in an Erlenmeyer flask with a stopper, and further 25 ml of 75% ethanol was precisely sucked. After weighing the weight, the reduced weight was replenished with 75% ethanol, shaken uniformly, filtered, 10 ml of the filtrate was accurately weighed, put in a water bath pan, and dried by steam. The dried residue was fractionally dissolved with methanol, transferred to a 10 ml volumetric flask one by one, methanol was added to a predetermined scale and shaken uniformly to obtain a test product solution. Chromatography column: Phenomenex Gemini C18 (250 mm×4.6 mm, 5 μm), mobile phase: acetonitrile-0.1% phosphoric acid water (gradient elution), mobile phase: acetonitrile-0.2% phosphoric acid solution (35:65). ), detection wavelength: 245 nm, flow rate: 1.0 ml/min, column temperature: 40°C. The sample injection amount was 10 uL, and the psoralen contents in the standard product and the test product were measured.

4. Orthogonal test optimization result and analysis When the orthogonal test result (see Table 2 for details) was analyzed, the influence of each factor on the extraction efficiency was the number of decoctions> the amount of water added> the degree of crushing> the decoction time. , The optimum level combination of the extraction process was found to be A ₃ B ₁ C ₃ D ₂ . From the results of the analysis of variance in Table 3-1, it was found that the extraction was significantly influenced by the number of times of decoction and the amount of water added, and the effects of the decoction time and the crushing degree were not significant. Therefore, from the viewpoint of improving work efficiency, in the water extraction process, water was added to the crude drug 8 times and decocted 3 times per hour.

Table 1 Factors and Levels of Orthogonal Test of Water Decoction Process Conditions of Zhuwan Family Pharmaceutical Composition for Blood Supplementation
Note: 1. Decoction time: each time 2. Amount of water added: multiple of the total weight (g) of prescription crude drug

Table Table 2 Hoketsu YoTadashi for Zhuang water decoction process conditions orthogonal test L _{9 (3} ⁴⁾ of the pharmaceutical composition and its optimization results

Table 3-1 Table of analysis of variance for orthogonal test of water decoction process conditions
F _0.01 (2,2)=99.0, F _0.05 (2,2)=19.0

5. Orthogonal test Optimization test confirmation test Add 8 times water to the decoction decocted three times according to the above process, decoct for 1 hour, filter the decoction, concentrate to 1000 ml, measure 50 ml precisely, and poralen above As a result of measuring according to the content measuring method, the HPLC peak of psoralen was small and 1.5% of dry paste was obtained, which showed that when the crude drug was decocted 3 times, the active ingredient was basically extracted.

(3) Optimization of the drying process of concentrated paste The concentrated paste obtained by concentrating this product by the optimized water extraction process is dried under reduced pressure at 70°C, 80°C, and 90°C to a constant weight, and about 1 g of dried paste The sample psoralen content was measured according to the above psoralen measurement method, and the results are shown in Table 3-2. The experimental results show that the drying temperature affects the psoralen content of this product, vacuum drying at 80°C is optimal, and the working efficiency can be improved without lowering the psoralen content. As the drying temperature condition of the thick paste, it was decided to perform vacuum drying at 80°C.

Table 3-2, Drying temperature test of thick paste

(4) Study on optimization of granulation molding process When the drug solution obtained according to the optimum extraction process is concentrated and dried to obtain an extract powder, and an excipient is uniformly mixed with the extract powder to produce granules. Appropriate excipients were selected depending on the yield, solubility and mouthfeel of the. The results are shown in Table 4-1.

Measurement of granule yield: The prepared granules were weighed. Granule yield=weight g of accepted granules/(weight g of extract powder+weight g of excipient)×100%
Solubility measurement: Add precisely weighed granules to a 5 mL centrifuge tube dried to a certain weight, add 5 mL of boiling water precisely, stir for 5 minutes, shake, centrifuge at 3000 r/min for 15 minutes, The clean liquid was discarded, the residue was dried to a constant weight at 80° C., weighed precisely, and the dissolution rate was calculated. Dissolution rate = mass of dissolved granules / mass of granules x 100%
Mouthfeel grade: Sweetness with heavy bitterness and slight bitterness.

Table 4-1 Excipient screening results
Based on the results of the excipient screening experiment, the excipient was sucrose.

Consideration of molding process:
Of the many factors affecting the molding process, the concentration of ethanol, the amount and factor levels in consideration of the stirring time and L ₉ ethanol _{(3 4)} Design the orthogonal test table, Table 2-2, Table 2 -3. The moldability was used as an evaluation index.

Table 4-2 Factor level table

Table 4-3 L ₉ (3 ₄ ) orthogonal test table

Table 4-4 ANOVA results
Note: F _0.05 (2.2)=19.00
From the analysis of variance analysis, it was found that the degree of influence of the three factors on the yield of granules was A>B>C, and the factors A and B were significant. From the results of visual analysis, it was confirmed that the optimum process was A ₂ B ₂ C ₃ , and when the optimum process condition A ₂ B ₂ C ₃ test was repeated, the granule yield was almost the same as the test result in the orthogonal table. Matched See Tables 4-5. Therefore, the molding process was carried out with an ethanol concentration of 75%, an amount of ethanol used of 8%, and stirring for 15 minutes.

Table 4-5 List of verification experiment results (n=3)

(5) Examination on pilot test According to the screened prescription process, 100 times expansion was performed and three pilot tests were performed, and the batch numbers were set to 201604401, 201640402, and 201660403, respectively. In each batch, raw materials were weighed at a rate of 100,000 g. Add 8 times amount of water, extract 3 times for 60 min each time, filter, concentrate, vacuum dry, pulverize and sieve to 100 mesh sieve, add sucrose up to 100000g to dry paste powder, homogenize To 75% ethanol to make a soft material, granulate through a 12-mesh sieve, dry at 75°C for 4 h, sieving through a 12-mesh sieve, uniformly mix with a mixer, and package. The granule yield, particle size and solubility of each of the obtained granule samples after packaging were statistically analyzed, and the obtained results are shown in the table.

Table 5 Results of 3 batch pilot test

(6) Stability investigation test In order to provide a basis for studying the stability of this product during packaging, storage and transportation, and for setting the expiration date of the drug product, "Technical Guiding Principles of Kampo Medicine and Natural Drug Stability Research" According to the requirements described in 1 above, the accelerated stability test and the long-term stability test were carried out on three samples of Ryurantsugran granule according to the relevant regulations of the granule stability test and the draft of the quality standard of this product. As a result of conducting a long-term room temperature investigation for 12 months and an accelerated stability investigation for 6 months for this product, the properties, identification, inspection, content measurement, and microbial limit inspection of all three samples satisfied the regulations. From this result, it is shown that the shelf life of this product can be provisionally set to 2 years, the storage condition is 30°C ± 2°C, the relative humidity is 65% ± 5%, and it is for packaging low-density polyethylene pharmaceutical products. The composite film was used as the packaging material.

(7) Conclusion The optimal process for preparing a Zhuang family pharmaceutical composition for blood supplementation is to add 8 times water to crude drug, decoct 3 times per hour, filter, concentrate, and dry under reduced pressure at 80°C. , Crush and sieve with 100 mesh, add sucrose to dry paste powder, mix evenly, make a soft material with 75% ethanol (8% of ethanol used), stir for 15 min, and make with 12 mesh sieve. Granules are dried at 75° C. for 4 hours, sieved with a 12-mesh sieve, sized, uniformly mixed with a mixer, and packed and packaged.

Second, clinical example Example 1
1. The Zhuang family pharmaceutical composition contains, by weight, 60 g of Kotomotou, 50 g of Futotou, 60 g of Scutellaria baicalensis, 50 g of ginseng, 30 g of kiban, 50 g of longan, 40 g of denshichi, 25 g of seisei, and 30 g of senbou.

2. Method for preparing Zhuang pharmaceutical composition:
He, female, 32 years old, Kawahito, 3 years old, after taking this Zhuang pharmaceutical composition according to the above-mentioned administration method, the symptoms improved.

Kim, female, 27 years old, Tamabayashi, 3 years old, after 3 years of vomiting, after taking this Zhuang pharmaceutical composition according to the above-mentioned administration method, the symptoms improved.

Example 2
1. The Zhuang family pharmaceutical composition contains, by weight, 50 parts of Koshimotou, 40 parts of Futoto, 20 parts of Senbutsu, 30 parts of carrots, 30 parts of kiban, 20 parts of longan, 20 parts of denshi, 20 parts of seisei, and 20 parts of senbou. .. The ratio was 5:4:2:3:3:2:2:2:2.

2. Method for preparing Zhuang pharmaceutical composition:
After washing and selecting each of the raw materials weighed out in parts by weight, water is added to the crude drug 8 times, decocted 3 times per hour, filtered, concentrated, dried under reduced pressure at 80° C., and crushed. Sucrose to dry paste powder, mix evenly, make a soft material with 75% ethanol (ethanol usage 8%), stir for 15 min, granulate with 12 mesh sieve, It was dried at 75° C. for 4 hours, sized with a 12-mesh sieve, uniformly mixed with a mixer, packed and packaged to give 30 g per pack.

3. Dosing method: The above granules were orally administered once per pack, once in the morning and once in the morning, and treated for 14 consecutive days, 2 courses in succession.

4. Clinical statistical data on patients who have used the drug of the present invention:
4.1 Basic data Diagnostic criteria: (1) Refer to the modern medical diagnostic criteria (developed according to the diagnostic criteria of the “Ahealth Chinese Medicine Clinical Guide”), and the subject's chief complaint makes the complexion pale and the object clearly visible with dark eyes. Discomfort, including palpitation, sleeplessness, and white nails, repeated bouts of more than 3 months, no significant organic or mental illness, no medication required, and related laboratory and physical examinations There are no positive results. (2) Refer to the Kampo medicine diagnostic criteria (2002 edition “Principles of New Chinese Medicine Clinical Research Guidance”), the main symptom of blood loss is pale complexion, dizziness, heart palpitations, and secondary symptoms are limb, asahi and nails. A person with white, insomnia, thin veins or voids, helplessness, pale tongue, white moss, two main symptoms and one secondary symptom was determined to be bloodless. The number of patients who met the sub-health examination criteria and the Kampo medicine blood deficiency criteria was 53 males and 67 females, for a total of 120 cases, and the age was 28 to 48 years and the medical condition was 2 to 9 years.

4.2 Grouping of Experiments Patients were randomly divided into two groups, an observation group and a control group, and each group had 60 patients.

4.3 Treatment plan Observation group: The above-mentioned granules are orally administered once per pack and once in the morning and evening, and one course is given for 14 days, and two courses are continuously treated.

Control group: Polysaccharide iron complex capsules 0.15 g and vitamin C 50 mg were orally administered once a day, and continuous treatment for 2 weeks was 1 course. Regarding the control group and the observation group, changes in the medical condition were observed after 3 courses of medication.

5. Judgment of therapeutic effect Refer to the therapeutic effect judgment standard for blood-subtype subhealth described in "Criteria for therapeutic effect evaluation of Kampo medicine signs". Short-term healing: The symptoms diagnosed by a Chinese medicine doctor disappear or almost disappear. Remarkable effect: The symptoms diagnosed by Chinese medicine doctors are significantly improved. Effective: All the symptoms diagnosed by the Kampo doctor were improved but not significant. Ineffective: Symptoms diagnosed by a Kampo doctor are not significantly improved and are exacerbated.

6. Statistical processing The analysis was performed using SPSS20.0 statistical software, the chi-square test was performed on the count data, and the t-test was performed on the measurement data. Differences were considered statistically significant if P<0.05.

7. Experimental Results The total effective rate of the observation group was 91.67%, and the total effective rate of the control group was 80.00%. The difference in the observation group was statistically significant when compared with the control group. The clinical treatment effect of the observation group was superior to that of the control group.

Table 6 Comparison of clinical treatment effect between control group and observation group
Note, P<0.05 when compared with the control group.

8. Typical illness Mr. Tan, female, 36 years old, Nanning city, 3 years old, after taking this Zhuang pharmaceutical composition according to the above administration method, the symptoms improved.
After receiving this Zhuang pharmaceutical composition according to the above administration method, the symptoms improved.

Claims (4)

20 to 60 parts by weight of Koshimato, 10 to 50 parts by weight of sardine, 10 to 60 parts by weight of sardine, 10 to 50 parts by weight of carrot, 10 to 30 parts by weight of kiban, 10 to 50 parts by weight of longan, 10 to 40 parts by weight of denshi, seisei Prepared using 10 to 25 parts by weight and 10 to 30 parts by weight of senbou,
A Zhuang pharmaceutical composition for blood supplementation, which is characterized in that: Using 50 parts by weight of Koshimoto, 40 parts by weight of futou, 20 parts by weight of sardines, 30 parts by weight of carrot, 30 parts by weight of longan, 20 parts by weight of longan, 20 parts by weight of denshi, 20 parts by weight of senbou, and 20 parts by weight of senbou Prepared,
The Zhuang family pharmaceutical composition for blood supplementation according to claim 1, wherein After washing each of the raw materials weighed in the above-mentioned parts by weight, water was added to the crude drug 8 times and decocted for 1 hour each time, decocted 3 times, filtered, concentrated, and dried under reduced pressure at 80°C. , Crushed and sieved with 100 mesh, add sucrose to dry paste powder, mix evenly, prepare soft material with ethanol with purity of 75% (ethanol dose is 8%), stir for 15 minutes , and mix with 12 mesh Sieving, granulating, drying at 75° C. for 4 hours , sieving with a 12 mesh sieve, uniform mixing with a mixer, packing and packaging,
The method for preparing a Zhuang family pharmaceutical composition for blood supplementation according to claim 1 or 2, characterized in that. The dosage form of the Zhuang pharmaceutical composition is a decoction or a granule,
The method for preparing a Zhuang family pharmaceutical composition for blood supplementation according to claim 3, wherein.