JP6694434B2 - 信号増幅を備えた圧電薄膜共振器 - Google Patents
信号増幅を備えた圧電薄膜共振器 Download PDFInfo
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Description
本願は、2014年9月15日に提出された米国特許仮出願第62/050,589号の恩恵を主張するものであり、この出願は、本明細書に示す開示と矛盾しない範囲でその全内容が参照により本明細書に組み込まれる。
(i)入力電気信号をTFBARと結合させ、ここで入力電気信号は圧電共振器の共振帯内の1つの相及び1つの周波数を有し、当該周波数は、約500MHz以上(例えば約700MHz以上、約800MHz以上、約900MHz以上、約1GHz以上、約2GHz以上、約2.2GHz以上、約2.4GHz以上、約2.5GHz以上、約500MHz〜約4GHz、約800MHz〜約3GHz、約800MHz〜約10GHz、又は約2GHz〜約2.5GHz)である;
(ii)入力電気信号をTFBARを通じて送信して、1つの周波数及び1つの相を有する出力電気信号を生成し;
(iii)TFBARからの出力電気信号を受信し;そして
(iv)TFBARの表面に加えられた質量により引き起こされる出力電気信号の周波数又は相の変化を決定する、ここで相の周波数の変化は、TFBARの表面に加えられた質量の尺度と見做す;ことにより測定される。
Claims (12)
- 試料中の解析物を検出する方法であって、以下の工程:
解析物又は解析物及びタグを連結した解析物分子である第一の認識成分と、増幅要素を連結した第二の認識成分とを接触させて、第一の認識成分及び増幅要素を連結した第二の認識成分を含む複合体を生成する工程、
ここで、第一の認識成分が圧電薄膜共振器(TFBAR)の表面に対して不動化され、解析物、タグが連結する解析物分子、又はタグ、又は第二の認識成分に結合するこれらのいずれかの分子の1つ以上を選択的に結合するように構成され、
ここで、増幅要素が連結した第二の認識成分は、解析物、タグが連結する解析物分子、又はタグ、又は第一の認識成分に結合するこれらのいずれかの分子の1つ以上を選択的に結合するように構成され、かつ
ここで、第二の認識成分は第一の結合パートナーと会合され、増幅要素は第一の結合パートナーと選択的に結合するように構成された第二の結合パートナーと会合され、増幅要素は第一と第二の結合パートナーとの間の結合を介して第二の認識要素に連結される;
連結した増幅要素を、増幅前駆体をTFBARの表面の質量を増加させる分子に変換する条件下で、増幅前駆体と接触させる工程;及び
TFBARの表面に追加された質量を測定する工程;
を含む、方法。 - 前記解析物、又は解析物及びタグが連結した解析物が、TFBARの表面に対して不動化された第一の認識成分と接触させられる前に、増幅要素と連結した第二の認識成分と接触させられる、請求項1に記載の方法。
- 前記解析物、又は解析物及びタグが連結した解析物が、増幅要素と連結した第二の認識成分と接触させられる前に、第一の認識成分と接触させられる、請求項1に記載の方法。
- 前記解析物、又は解析物及びタグが連結した解析物、第一の認識成分及び増幅要素と連結した第二の認識成分が、同時に接触させられる、請求項1に記載の方法。
- 前記TFBARの表面に追加された質量を測定する工程が:
前記TFBARに、前記TFBARの共鳴帯域内にある900MHz以上の周波数を有する入力電気信号を連結すること、
前記入力電気信号を前記TFBAR全体に送信して、所定周波数を有する出力電気信号を生成すること、
前記TFBARから前記出力電気信号を受信すること、および
前記TFBARの表面に沈殿物が沈殿することにより生じる前記出力電気信号の相シフトの変化を決定すること、
を含む、請求項1〜4のいずれか1項に記載の方法。 - 前記相シフトの変化は、挿入係数相シフトまたは反射係数相シフトの変化である、請求項5に記載の方法。
- 前記TFBARの表面に追加された質量を測定する工程が、
前記TFBARを作動させて、900MHz以上の周波数で振動させること、
前記TFBARの振動の共振特性を表す1種以上の共振器出力信号を測定すること、および
前記TFBARの作動周波数を調節して、前記TFBARの共振点を維持することを含む、請求項1〜4のいずれか一項に記載の方法。 - 前記TFBARの共振点は最大群遅延の点である、請求項7に記載の方法。
- 前記周波数が1.8GHz以上、2GHz以上、800MHz〜10GHz、又は2GHz〜2.5GHzである、請求項5〜8のいずれか1項に記載の方法。
- 前記増幅要素が酵素であり、前記増幅前駆体が基質であり、当該酵素が当該基質を沈殿に変換するように構成されている、請求項1〜9のいずれか1項に記載の方法。
- 試料中の解析物を検出する系であって:
第一の認識成分が不動化される表面を有する圧電薄膜共振器(TFBAR)、ここで第一の認識成分は、解析物、タグが連結する解析物分子、又はタグ、又は増幅要素が連結した第二の認識成分が結合するこれらのいずれかの分子を選択的に結合するように構成され、TFBARの共振周波数が900MHz以上である;
増幅分子、前記増幅要素が連結した第二の認識成分又はその成分、及び任意で1つ以上のタグ、及び解析分子を収納する1つ以上の容器、ここで当該増幅要素が連結した第二の認識成分が、第一の結合パートナーと会合された第二の認識成分、及び第一の結合パートナーに選択的に結合するように構成された第二の結合パートナーに会合された増幅要素を含み、ここで、前記増幅要素が、第一と第二の結合パートナーとの間の結合を介して第二の認識要素に連結される:
前記1つ以上の容器から、第一の認識成分が結合しているTFBARの表面への流路;
前記TFBARを作動させて振動させる作動回路;
前記TFBARに連結されるように配置され、前記TFBARの振動の共振特性を表す1種以上の共振器出力信号を測定するように構成された測定回路;及び
作動的に前記作動回路および前記測定回路と連結された制御部;
を含む、系。 - 試料中の解析物を検出するデバイスに使用されるキットであって:
第一の認識成分が不動化される表面を有する圧電薄膜共振器(TFBAR)、ここで第一の認識成分は、解析物、タグが連結する解析物分子、又はタグ、又は増幅要素が連結した第二の認識成分が結合するこれらのいずれかの分子を選択的に結合するように構成され、TFBARの共振周波数が900MHz以上である;及び
増幅分子、前記増幅要素が連結した第二の認識成分又はその成分、及び任意で1つ以上のタグ、及び解析分子を収納する1つ以上の容器、ここで当該増幅要素が連結した第二の認識成分が、第一の結合パートナーと会合された第二の認識成分、及び第一の結合パートナーに選択的に結合するように構成された第二の結合パートナーに会合された増幅要素を含み、ここで、前記増幅要素が、第一と第二の結合パートナーとの間の結合を介して第二の認識要素に連結される:
を含む、キット。
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