JP6692049B2 - 識別装置および識別方法 - Google Patents
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1429—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its signal processing
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
- G01B11/00—Measuring arrangements characterised by the use of optical techniques
- G01B11/02—Measuring arrangements characterised by the use of optical techniques for measuring length, width or thickness
- G01B11/06—Measuring arrangements characterised by the use of optical techniques for measuring length, width or thickness for measuring thickness ; e.g. of sheet material
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- G01N15/02—Investigating particle size or size distribution
- G01N15/0205—Investigating particle size or size distribution by optical means, e.g. by light scattering, diffraction, holography or imaging
- G01N15/0227—Investigating particle size or size distribution by optical means, e.g. by light scattering, diffraction, holography or imaging using imaging, e.g. a projected image of suspension; using holography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N15/10—Investigating individual particles
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- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
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- G06T7/0012—Biomedical image inspection
- G06T7/0014—Biomedical image inspection using an image reference approach
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
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- G06V20/00—Scenes; Scene-specific elements
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/02—Investigating particle size or size distribution
- G01N15/0205—Investigating particle size or size distribution by optical means, e.g. by light scattering, diffraction, holography or imaging
- G01N2015/025—Methods for single or grouped particles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
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Description
Claims (8)
- 細胞の光学的厚み分布の画像の特徴量を抽出する特徴量抽出部と、
種別が既知である既知細胞の光学的厚み分布の画像について前記特徴量抽出部により抽出された特徴量に基づいて機械学習を行った学習結果を記憶する記憶部と、
前記記憶部により記憶されている学習結果を用いて、種別が未知である未知細胞の光学的厚み分布の画像について前記特徴量抽出部により抽出された特徴量に基づいて未知細胞の種別を判定する識別部と、
を備え、
未知細胞の光学的厚み分布の画像の特徴量を抽出する際、または、未知細胞の種別を判定する際に、前記記憶部により記憶されている学習結果を用いるとともに、
前記特徴量抽出部が、光学的厚み分布の画像内の位置における光学的厚みの空間的変化量に関する情報を該画像の特徴量として抽出し、前記光学的厚みの空間的変化量に関する情報は、前記光学的厚み分布の画像内の位置における光学的厚みの傾き情報を表すベクトルの勾配強度および勾配方向の双方または何れか一方である、
識別装置。 - 既知細胞の光学的厚み分布の画像について前記特徴量抽出部により抽出された特徴量に基づいて機械学習を行う学習部を更に備え、
前記記憶部が、前記学習部による機械学習の学習結果を記憶する、
請求項1に記載の識別装置。 - 前記特徴量抽出部は、前記記憶部により記憶されている学習結果を用いて、前記未知細胞の光学的厚み分布の画像内において前記特徴量を抽出する領域を少なくとも1つ設定する、請求項1または2に記載の識別装置。
- 種別が未知である未知細胞の光学的厚み分布の画像の特徴量を特徴量抽出部により抽出する第1特徴量抽出ステップと、
種別が既知である既知細胞の光学的厚み分布の画像について前記特徴量抽出部により抽出された特徴量に基づいて機械学習を行って記憶部により記憶された当該学習結果を用いて、前記第1特徴量抽出ステップにおいて抽出された特徴量に基づいて未知細胞の種別を判定する識別ステップと、
を備え、
未知細胞の光学的厚み分布の画像の特徴量を抽出する際、または、未知細胞の種別を判定する際に、前記記憶部により記憶されている学習結果を用いるとともに、
前記特徴量抽出部により、光学的厚み分布の画像内の位置における光学的厚みの空間的変化量に関する情報を該画像の特徴量として抽出し、前記光学的厚みの空間的変化量に関する情報は、前記光学的厚み分布の画像内の位置における光学的厚みの傾き情報を表すベクトルの勾配強度および勾配方向の双方または何れか一方である、
識別方法。 - 既知細胞の光学的厚み分布の画像の特徴量を前記特徴量抽出部により抽出する第2特徴量抽出ステップと、
前記第2特徴量抽出ステップにおいて抽出された特徴量に基づいて機械学習を行い、その学習結果を前記記憶部に記憶させる学習ステップと、
を更に備える、請求項4に記載の識別方法。 - 前記第1特徴量抽出ステップは、前記記憶部により記憶された学習結果を用いて、前記未知細胞の光学的厚み分布の画像内において前記特徴量を抽出する領域を少なくとも1つを設定する、請求項4または5に記載の識別方法。
- 前記細胞として白血球およびがん細胞を含む、請求項4〜6の何れか一項に記載の識別方法。
- 前記特徴量抽出部により、溶血剤を添加した細胞の光学的厚み分布の画像の特徴量を抽出する、請求項7に記載の識別方法。
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WO2017130640A1 (ja) * | 2016-01-28 | 2017-08-03 | 株式会社リコー | 画像処理装置、撮像装置、移動体機器制御システム、画像処理方法、及びプログラム |
US20200251184A1 (en) * | 2016-12-16 | 2020-08-06 | Osaka University | Classification analysis method, classification analysis device, and storage medium for classification analysis |
CN110392732B (zh) * | 2017-03-02 | 2023-07-28 | 株式会社岛津制作所 | 细胞分析方法和细胞分析装置 |
JP6807529B2 (ja) * | 2017-05-07 | 2021-01-06 | アイポア株式会社 | 識別方法、分類分析方法、識別装置、分類分析装置および記憶媒体 |
US20200193140A1 (en) * | 2017-08-24 | 2020-06-18 | Nano Global | Detection of Biological Cells or Biological Substances |
JP6999129B2 (ja) | 2017-09-06 | 2022-01-18 | 浜松ホトニクス株式会社 | 細胞観察システムおよび細胞観察方法 |
JP6798625B2 (ja) * | 2017-11-14 | 2020-12-09 | 株式会社ニコン | 定量位相画像生成方法、定量位相画像生成装置およびプログラム |
CN111630604A (zh) * | 2017-11-20 | 2020-09-04 | 纳诺全球公司 | 基于生物细胞或生物物质的检测的数据收集和分析 |
JP6732722B2 (ja) * | 2017-12-11 | 2020-08-05 | 憲隆 福永 | 胚選抜システム |
JP7000379B2 (ja) * | 2019-05-07 | 2022-01-19 | 憲隆 福永 | 胚選抜システム |
CN111105416B (zh) * | 2019-12-31 | 2022-09-09 | 北京理工大学重庆创新中心 | 一种骨髓细胞增生程度自动分级方法及系统 |
CN111784669B (zh) * | 2020-06-30 | 2024-04-02 | 长沙理工大学 | 一种胶囊内镜图像多病灶检测方法 |
WO2022024564A1 (ja) | 2020-07-30 | 2022-02-03 | 浜松ホトニクス株式会社 | 判別装置、判別方法、判別プログラム及び記録媒体 |
JP2022039514A (ja) | 2020-08-28 | 2022-03-10 | 浜松ホトニクス株式会社 | 学習モデル生成方法、識別方法、学習モデル生成システム、識別システム、学習モデル生成プログラム、識別プログラム及び記録媒体 |
WO2022195754A1 (ja) * | 2021-03-17 | 2022-09-22 | 株式会社エビデント | データ処理方法、データ処理装置、三次元観察装置、学習方法、学習装置及び記録媒体 |
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