JP6690220B2 - Agent for hair - Google Patents

Description

  The present invention relates to a preventive or ameliorating agent for gray hair caused by aging, stress, etc., a bFGF (basic fibroblast growth factor, FGF-2) production promoter.

The pigment of body hair is produced by pigment cells existing in the bulb portion, and the hair mother cells supplied with this pigment form body hair, so that the pigment-containing body hair is generated. When the hair grows, the bulb part disappears, and the pigment cells in the bulb part also disappear with it. When a new hair cycle begins and a new body hair is generated, pigment cells are supplied from the pigment stem cells existing in the bulge region, so that the pigmented body hair is generated again. Pigment stem cells are reduced or depleted due to aging and various stresses, and problems occur in the pigment cell supply process. Occurs.
Hair dyes are generally used as a coping method for gray hair, but the annoyance that requires continuous hair dyeing, damage to hair due to its irritative properties, contact dermatitis, etc. are not always sufficient. I can't say.
As a preventive or ameliorating agent for gray hair, many substances have been found with the action of activating enzymes such as tyrosinase involved in pigment synthesis of pigment cells as an index (Patent Documents 1 to 5, Non-Patent Documents). 1). However, even if the pigment production system is activated, if the number of pigment cells in the hair bulb is insufficient in the first place, the effect of the substance found by such an index cannot be expected so much.

  In order to prevent gray hair, it is considered important to prevent the decrease of pigment stem cells, promote the transfer to hair matrix, and increase the number of decreased pigment matrix of hair matrix. Since bFGF is known as a factor that exerts a cell proliferation effect on all cells of the pigment cell lineage (Non-patent Document 2), it suppresses the decrease of pigment cells in the bulb part and pigment stem cells in the bulge region and also It is considered to be effective in increasing or preventing gray hair. In fact, it has been clarified that applying bFGF to the scalp exerts a gray hair preventing or improving effect (Patent Document 6). Furthermore, it has been clarified that a substance that acts on dermal papilla cells and promotes expression of factors including bFGF from dermal papilla cells prevents and improves gray hair (Patent Document 7). In order to act on pigment cells in the hair bulb and pigment stem cells in the bulge region, it is important to interact with various cells that make up the hair follicle and promote bFGF production in hair papilla cells, which is called the control tower of the hair follicle. Is.

Patent 4433241 JP 2001-131032 Patent 4921732 JP 2011-157317 JP 2002-47130 JP 5-43424 JP2003-171240 JP2010-126475

Hideaki Matsuda et al., "Anti-Aging Series 1 Practical Practice of Gray Hair, Hair Loss, and Hair Growth" NTS, July 4, 2005, p.37-48 Hirobe T et al., Life cycle of human melanocytes is regulated by endothelin-1 and stem cell factor in synergy with cyclic AMP and basic fibroblast growth factor, J Dermatol Sci, 57 (2), 123-131, (2010) Sasaki S et al., Influence of prostaglandin F2alpha and its analogues on hair regrowth and follicular melanogenesis in a murine model, Exp Dermatol, 14 (5), 323-328, (2005) Bellandi S et al., Repigmentation of hair after latanoprost therapy, J Eur Acad Dermatol Venereol, 25 (12), 1485-1487, (2010)

  An object of the present invention is to provide means for preventing or improving gray hair caused by aging, stress and the like. Another object of the present invention is to provide a material that promotes the production of bFGF.

  Therefore, as a result of diligent studies, the inventors of the present invention have found that the psyllium extract extracted with a solvent containing a polyhydric alcohol has an excellent effect of improving or preventing gray hair, and that the effect is due to a very high bFGF production promoting effect. Heading out, the present invention has been completed.

That is, the present invention is
(1) A preventive or improver for gray hair, which contains, as an active ingredient, an oat extract extracted with a solvent containing polyhydric alcohol
(2) The agent for preventing or improving gray hair according to (1), which further contains a hair growth substance,
(3) The agent for preventing or improving gray hair according to (1) or (2), which is for external use on the skin.
(4) A bFGF production promoter, which contains, as an active ingredient, Oat extract extracted with a solvent containing polyhydric alcohol,
(5) The bFGF production-promoting agent according to (4), which has a bFGF production-promoting effect and is labeled as being used to promote the production of bFGF.
Is.

  The agent for preventing or improving gray hair of the present invention can prevent or improve the occurrence of gray hair caused by aging, stress, etc. by promoting the production of bFGF in hair papilla cells. Further, it is possible to provide a novel material capable of promoting the production of bFGF.

FIG. 1 is a graph showing the relative expression level of bFGF in Test Example 1. In the figure, Oat B is an Oat extract prepared by mixing 1,3-butylene glycol and water, and Oat E is an Oat extract prepared by mixing ethanol and water.

The psyllium used in the present invention is a crude drug derived from the bark excluding the pericarp of the genus Phellidendron amurense of the citrus family Rutaceae (Scienceae) (scientific name Phellodendron amurense).
The psyllium extract used in the present invention is extracted with a solvent containing a polyhydric alcohol (1,3-butylene glycol, propylene glycol, dipropylene glycol, glycerin). In addition to polyhydric alcohol, it may contain water, lower aliphatic alcohol (methanol, ethanol, isopropyl alcohol, etc.), lower aliphatic ketone (acetone, etc.), etc. Of these, a mixed liquid of polyhydric alcohol and water Most preferably, it is extracted with. The polyhydric alcohol is preferably 1,3-butylene glycol or propylene glycol, with 1,3-butylene glycol being most preferred. When extracting with a solvent composed of water and 1,3-butylene glycol, the content of 1,3-butylene glycol in the solvent is preferably 30 to 70% by volume. The form of the extract is not particularly limited, and it can be made into a dry extract powder, an extract powder, a soft extract, a flow extract, etc. by a treatment such as heat treatment, freeze-drying or vacuum drying.

  The agent for preventing or improving gray hair of the present invention can be provided as a cosmetic, a quasi drug, a drug, a food or drink, and the like. In addition, it can be used as a reagent. The dosage form is not particularly limited, but may be external use or internal use, and is preferably an external preparation applied to the skin including the scalp. Examples of dosage forms when the present invention is applied externally include lotions, liquids, creams, ointments, gels, sprays, shampoos, conditioners, soaps, etc. Examples of the form include tablets, powders, powders, granules, liquids, capsules, dry syrups, jellies, liquid foods, semi-solid foods, and solid foods. These can be manufactured by a known method. At the time of production, various additives that can be contained in cosmetics, quasi-drugs, pharmaceuticals, foods and drinks or reagents can be blended within a range that does not impair the effects of the present invention.

  Further, the preventive or improving agent for gray hair of the present invention is an assembly extract, carrot extract, capsicum tincture, ginger tincture, loquat leaf extract, glycyrrhetinic acid, dipotassium glycyrrhizinate, monoammonium glycyrrhizinate, pantothenic acid, panthenol, vitamin E and its. It can also be used in combination with a hair growth substance such as a derivative, hinokitiol, salicylic acid, piroctone olamine, minoxidil, adenosine, t-flavanone, cytopurine, pentadecanoic acid glyceride, allantoin or nicotinic acid amide. By combining them, the production of hair is accelerated, and the effect of preventing or improving gray hair can be obtained at an early stage.

  The compounding amount of the psyllium extract extracted with the solvent containing the polyhydric alcohol of the present invention is 0.000001 to 10% by mass relative to the entire composition when provided as a cosmetic, a quasi drug, a drug, a food or drink or a reagent. The amount is preferably 0.0001 to 5% by mass, more preferably 0.001 to 1% by mass.

  In addition, products containing the bFGF production promoter of the present invention (medicines, quasi drugs, cosmetics, foods and drinks, or reagents) or their instructions are labeled with an indication that they are used to promote bFGF production. Is. Here, "the product or its description is labeled" means that the product body, container, packaging, etc. is labeled, or a product information disclosure manual, package insert, advertising material, application This means that a display is attached to materials, other printed materials, advertisements, or the like. In addition, these indications can include information regarding use for prevention or treatment of diseases caused by promotion of bFGF production.

  Hereinafter, the present invention will be described more specifically with reference to Examples and Test Examples, but the present invention is not limited to the Examples.

(Test Example 1) Evaluation of bFGF production promoting action on human hair papilla cells <Test method>
Human dermal papilla cells were seeded at a density of 2 × 10 ⁴ cells / mL, and precultured for 2 days in an incubator set at 37 ° C. and CO ₂ 5%. Earle's MEM containing 12% FBS and a specified amount of penicillin-streptomycin was used as the medium. After pre-culturing, the cell surface was washed with PBS and then replaced with serum-free Earle's MEM. After further culturing for 1 day, a test substance or a control substance was added and culturing was continued for 24 hours. Then, the medium was removed by suction, lysate buffer was added to lyse the cells, and the cell lysate was collected. RNA was recovered from the cell lysate using the RNeasy Mini Kit (Qiagen) according to the attached protocol, and using this as a template, cDNA was synthesized by a reverse transcription reaction using Prime Script RT Master mix (Takara Bio). The mRNA expression levels of GAPDH and bFGF were measured by a real-time PCR system (Step One Plus, Applied Biosystems) using the synthesized cDNA, and the bFGF expression level was corrected by the GAPDH expression level. TaqMan Gene Expression Assays (GAPDH: Hs99999905_m1, bFGF: Hs00266645_m1, Applied Biosystems) were used as the primers and probes. In addition, as a test substance, an oat extract (Owaku B) extracted with a mixed solution of 50% by volume of 1,3-butylene glycol and 50% by volume of water and an extracted extract of Oataku (a mixture of 90% by volume of ethanol and 10% by volume of water). Oataku E) was used.

<Test results>
FIG. 1 shows the results of evaluating the bFGF production promoting effect of Oataku. The bFGF relative expression level of each group is shown when the bFGF relative expression level of the control group is 1. Oat extract extracted with a mixture of 1,3-butylene glycol and water at a concentration of 3 to 10 μg / mL significantly increased the expression level of bFGF in hair papilla cells compared with the control group. It is known that the prostaglandin F _2.alpha and its derivatives have white hair preventing or ameliorating action (Patent Document 8, Non-Patent Documents 3, 4), bFGF production-promoting effect by bark extract, prostaglandin F _2.alpha 10 [mu] M It was equal to or higher than (the concentration showing the maximum effect in this test system). On the other hand, the Oat extract extracted with the mixed solution of ethanol and water showed a higher value than that of the control group, but its effect was very weak as compared with the Oat extract extracted with the mixed solution of 1,3-butylene glycol and water.

  From the above results, it was revealed that the polyhydric alcohol extract of Oatuba has the effect of promoting bFGF production in hair papilla cells, and therefore, based on this action, the polyhydric alcohol extract of Oataku prevents gray hair or It was shown to have an improving effect.

  Since the extract of polyhydric alcohol of Oataku of the present invention has a bFGF production promoting action, it can be used in the fields of cosmetics, quasi-drugs, and foods and drinks for preventing and improving gray hair caused by aging, stress, etc. . In addition, the bFGF production promoter of the present invention can be used as a positive control drug in material screening and the like.

Claims (5)

An agent for preventing or improving gray hair, which comprises, as an active ingredient, an oat extract extracted with a solvent containing a polyhydric alcohol. Furthermore, the gray hair preventive or improving agent of Claim 1 containing a hair growth substance. The gray hair preventive or improving agent according to claim 1 or 2, which is for external use on the skin. A bFGF production-promoting agent comprising, as an active ingredient, an oat extract extracted with a solvent containing a polyhydric alcohol. The bFGF production-promoting agent according to claim 4, which has a bFGF production-promoting effect and is labeled as being used for promoting the production of bFGF.

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