JP6639812B2 - CIP cleaning method - Google Patents

Description

  The present invention relates to a CIP cleaning method using a CIP cleaning composition, and more particularly, to a CIP cleaning method for a production line of a food and beverage factory.

  In food and beverage manufacturing plants that manufacture foods such as beer, juice, dairy beverages, dairy products, frozen foods, retort foods, seasonings, mayonnaise, etc., they are cleaned by CIP cleaning for their manufacturing facilities and equipment. It is carried out. CIP cleaning is an acronym for “Cleaning in place”, which allows a cleaning liquid to flow as it is without disassembling manufacturing equipment and manufacturing equipment, and to fill and circulate the cleaning liquid. This is a cleaning method of spraying a cleaning liquid to wash and remove residual stains, and is also called stationary cleaning or stationary circulation cleaning.

  Conventionally, in CIP cleaning, alkali cleaning or cleaning combining acid cleaning with alkali cleaning is performed. Among them, food and beverage manufacturing plants produce organic and inorganic stains derived from products.To maintain high cleanliness, use a sequestering agent in the alkaline washing step, or use an alkaline washing step and an acid washing step. CIP cleaning is also performed in which an alkali cleaning and an acid cleaning steps are combined with a disinfection step using a disinfectant.

  Conventionally, various cleaning compositions and cleaning methods used for CIP cleaning have been proposed. For example, a method using a cleaning composition containing sodium hydroxide and glutamates (Patent Document 1), an alkali metal hydroxide salt , A composition containing an alkyl polyglucoside, a polyoxyalkylene ether and / or a polyoxyalkylene alkyl phenyl ether, a sequestering agent, and a high-molecular polymer (Patent Document 2). Further, those having a disinfecting function include those containing hypochlorite and isocyanuric acid or a salt thereof (Patent Document 3), alkali metal hydroxide salts, chlorinated oxidizing agents, silicates, phosphonates, amphoteric One containing a surfactant (Patent Document 4) is known.

Patent No. 4114820 JP 2006-265469 A Patent No. 5007203 JP 2010-215780 A

  However, the CIP cleaning methods disclosed in Patent Literature 1 and Patent Literature 2 are techniques of alkaline cleaning using a combination of an alkali such as sodium hydroxide and a sequestering agent, and are applied to CIP cleaning in a food and beverage manufacturing plant. In this case, it is difficult to sufficiently remove dirt, and it is necessary to further combine an acid cleaning step before and after the alkali cleaning step. However, when performing the CIP cleaning by combining the alkali cleaning step and the acid cleaning step, a rinsing step is required between the alkali cleaning step and the acid cleaning step, and there is a problem that the cleaning cycle becomes long. In particular, in recent years, the variety of foods and beverages has increased due to an increase in production volume due to improvement in production efficiency and diversification of customer preferences, and the frequency of CIP cleaning for switching products has increased. The necessity causes a significant decrease in production efficiency. Also, in the CIP cleaning methods described in Patent Documents 3 and 4, since a chlorine-based disinfectant such as hypochlorous acid is used, when the acid cleaning step is combined, sufficient rinsing step is required to prevent generation of toxic chlorine gas. There is a problem that rinsing is required and the washing cycle is further lengthened. Moreover, in a food and beverage manufacturing plant, dirt such as polysaccharides and biofilms is easily generated. However, it is difficult to remove dirt from polysaccharides and biofilms by the conventional CIP cleaning method. When performing the combined sterilization steps, there is a problem that washing at a low temperature with little chlorine inactivation degrades the washing property and the sterilization property.

  The present invention has been made to solve the above-mentioned conventional problems, and it is possible to simultaneously perform efficient removal of bacteria and inorganic bacteria and sterilization without performing an acid washing step, and furthermore, It is an object of the present invention to provide a CIP cleaning method capable of efficiently removing dirt and removing bacteria in a short time even if the cleaning is performed at a low temperature.

  In order to solve the above-mentioned problems, the present inventors have conducted intensive studies and as a result, have found that (A) an alkali metal hydroxide and / or an alkaline earth metal hydroxide, and (B) methylglycine diacetate or a salt thereof. Then, the mass ratio of the component (A) to the component (B) is (A) / (B) = 0.16 to 190. ) Addition of a CIP additive containing a chlorine-based disinfectant as a component to remove CIP containing 0.005 to 0.2% by mass of effective chlorine and 0.001 to 0.6% by mass of component (B). The present inventors have found that a method of CIP-cleaning a food and beverage production line by setting the temperature of a CIP sanitizing cleaning liquid to 10 to 60 ° C. using a bacterial cleaning liquid can solve the problems of the conventional CIP cleaning method, and complete the present invention. Reached.

That is, the present invention
(1) (A) an alkali metal hydroxide compound as a component, contains methylglycine diacetic acid or a salt thereof, (A) component (B) weight ratio of the component (B), (A) / (B ) = 0.16 to 190, prepared by adding a CIP additive containing a chlorine-based disinfectant as a component (C) to a diluted washing solution obtained by diluting a CIP detergent composition with water. CIP cleaning and disinfection of food and beverage production line with a CIP disinfecting cleaning solution at 10 to 60 ° C containing 0.005 to 0.2% by mass of effective chlorine and 0.001 to 0.6% by mass of component (B) A CIP cleaning method, wherein the cleaning is performed simultaneously;
(2) The CIP cleaning method of the above (1), wherein the component (A) contains at least one selected from sodium hydroxide and potassium hydroxide,
(3) The component (C) contains at least one or more selected from alkali metal hypochlorite and / or alkaline earth metal hypochlorite and chlorinated isocyanurate as a chlorine-based disinfectant. (1) or (2) the CIP cleaning method,
(4) Further, in the CIP cleaning composition containing the components (A) and (B), a polymer is used as the component (D), and the mass ratio of the components (B) and (D) is ( D) / (B) = the CIP cleaning method according to any one of the above (1) to (3), which is contained so as to be 0.05 to 10.
Is the gist.

  ADVANTAGE OF THE INVENTION The CIP washing | cleaning method of this invention can wash | clean and remove the organic dirt and inorganic dirt which generate | occur | produce in a food-and-drinks production line simultaneously without performing acid washing, and can also remove bacteria in one process. Cleaning is not required by combining the three steps of alkali cleaning, acid cleaning, and bacteria elimination, and the rinsing step between each step is also unnecessary.This shortens the cleaning cycle and reduces the cleaning time when switching between products. In addition, even when there are many types of food and beverage products, production can be performed with high production efficiency. In addition, since it is washed with a washing liquid at 10 to 60 ° C., there is no possibility that the disinfecting property due to chlorine inactivation may be reduced, and dirt such as polysaccharides and biofilms which are difficult to remove when washed at a low temperature can be efficiently treated. Can be washed and removed well.

  The component (A) used in the CIP cleaning method of the present invention includes alkali metal hydroxides such as sodium hydroxide and potassium hydroxide, and alkaline earth metal hydroxides such as calcium hydroxide and magnesium hydroxide. . The alkali metal hydroxide and the alkaline earth metal hydroxide can be used alone or in combination of two or more. As the component (A), sodium hydroxide and potassium hydroxide are preferable. The alkali metal hydroxide and / or alkaline earth metal hydroxide of the component (A) is preferably contained in the CIP detergent composition in an amount of 1 to 40% by mass, but is preferably contained in an amount of 3 to 36% by mass. More preferably, the content is most preferably 5 to 32% by mass. If the amount of the component (A) is less than 1% by mass, the detergency may decrease, and if it exceeds 40% by mass, the storage stability may decrease.

  As the component (B), methyl glycine diacetate, methyl glycine diacetate sodium salt, methyl glycine diacetate potassium salt and the like are used, and methyl glycine diacetate sodium salt and methyl glycine diacetate potassium salt are preferred. Component (B) methylglycine diacetate or a salt thereof is preferably contained in the CIP detergent composition at 0.001 to 40% by mass, more preferably 0.05 to 10.1% by mass. , 0.1 to 1% by mass. If the amount of methyl glycine diacetic acid or a salt thereof is less than 0.001% by mass, detergency may decrease, and if it exceeds 40% by mass, storage stability may decrease. The CIP detergent composition used in the present invention is preferably in the form of an aqueous solution containing water together with the components (A) and (B) from the viewpoint of easy handling. Examples of the water used in the aqueous solution include tap water and groundwater. , Industrial water, ion-exchanged water, distilled water and the like, and ion-exchanged water and distilled water are preferred from the viewpoint of storage stability.

  (C) Additives for CIP containing the chlorine-based disinfectant of the component include hypochlorites such as potassium hypochlorite and sodium hypochlorite, and hypochlorites such as potassium chlorite and sodium chlorite. Chlorates, potassium chlorates, chlorates such as sodium chlorate, potassium perchlorates, perchlorates such as sodium perchlorate, dichloroisocyanurates such as sodium dichloroisocyanurate, potassium dichloroisocyanurate, etc. Those containing a chlorine-based disinfectant may be mentioned. The additive for CIP may contain one or more of these chlorine-based disinfectants. As the chlorine-based disinfectant, alkali metal hypochlorite, alkaline earth metal hypochlorite, and dichloroisocyanurate are preferable, and it is preferable to use one or a combination of two or more thereof. The additive for CIP of the component (C) may be composed of only the chlorine-based disinfectant or may be an aqueous solution of the chlorine-based disinfectant. In addition, an additive for CIP in which at least one kind of an alkali such as sodium hydroxide, a polymer, or a silicate is further added to the aqueous solution of the chlorine-based disinfectant or the chlorine-based disinfectant is also used as the component (C). Can be used. The additive for CIP as the component (C) can be used in any form of powder, liquid, or solution diluted with water, but liquid or solution is preferred from the viewpoint of easy handling. Examples of the water used for the solution include ion-exchanged water, distilled water, pure water, soft water, and tap water. From the viewpoint of storage stability, ion-exchanged water, distilled water, pure water, and the like are preferable.

  In the CIP cleaning method of the present invention, the CIP cleaning composition having a mass ratio of the component (A) to the component (B) of (A) / (B) = 0.16 to 190 is diluted with water. The cleaning solution was prepared by adding a CIP additive containing a chlorine-based disinfectant as a component (C). The food / beverage product manufacturing line is washed with a CIP sterilization washing solution containing 10% by mass of 10 to 60 ° C. Examples of water for diluting the CIP cleaning composition include tap water, ground water, industrial water, ion-exchanged water, distilled water, and the like, and tap water and ground water are preferred from the viewpoint of economy. When the mass ratio (A) / (B) of the component (A) and the component (B) of the cleaning composition for CIP is less than 0.16, the effective chlorine stability may decrease. In some cases, the cleaning properties of inorganic soils and polysaccharide stains are reduced. The mass ratio of the component (A) to the component (B) is preferably (A) / (B) = 1 to 100, and more preferably 5 to 80. Further, the CIP disinfecting and washing solution contains 0.005 to 0.2% by mass of effective chlorine, preferably 0.01 to 0.15% by mass, and more preferably 0.02 to 0.12% by mass. %. If the amount is less than 0.005% by mass, the bactericidal property may decrease. If the amount exceeds 0.2% by mass, the metal corrosion inhibiting property may decrease. The proportion of the component (B) in the CIP sanitizing and washing solution is 0.001 to 0.6% by mass, more preferably 0.01 to 0.55% by mass, and even more preferably 0.1 to 0% by mass. 0.5% by mass. When the proportion of the component (B) is less than 0.001% by mass, the CIP sanitizing cleaning solution is inferior in the detergency of organic substances, and when it exceeds 0.6% by mass, the effective chlorine stability decreases, and However, the detergency of the CIP disinfecting cleaning solution is saturated, resulting in poor economy. Note that the effective chlorine amount of the CIP disinfecting washing solution is the amount immediately after preparing the CIP disinfecting washing solution. In addition, the effective chlorine amount was measured based on the residual chlorine-iodine titration method of JIS K0101.

  If the CIP disinfecting cleaning solution used in the CIP cleaning method of the present invention further contains a high molecular polymer as the component (D) in addition to the component (A), the component (B), and the component (C), scale adhesion during cleaning is suppressed. Has the effect. The component (D), together with the component (A) and the component (B), in the detergent composition for CIP, the mass ratio of the component (B) to the component (D) is (D) / (B) = 0.01 to It is preferable to mix it so that it may be set to 10, and it is more preferable to mix | blend so that it may become (D) / (B) = 0.05-2.

  Examples of the high molecular weight polymer as the component (D) include polyacrylic acid, polymethacrylic acid, polymaleic acid, polyitaconic acid, acrylic acid-methacrylic acid copolymer, acrylic acid-maleic acid copolymer, and olefin-maleic acid copolymer. , Acrylic acid-sulfonic acid copolymer, maleic anhydride-styrene copolymer, maleic anhydride-ethylene copolymer, maleic anhydride-vinyl acetate copolymer, maleic anhydride-acrylic acid ester copolymer, etc. These salts include polyacrylic acid or a salt thereof, polymaleic acid or a salt thereof, acrylic acid-methacrylic acid copolymer, acrylic acid-maleic acid copolymer, olefin-maleic acid copolymer, acrylic acid -Sulfonic acid copolymers are preferred. These polycarboxylic acid type polymers or salts thereof may be used alone or in combination of two or more. As a copolymer such as an acrylic acid type copolymer, a maleic acid type copolymer, and a methacrylic acid type copolymer, those containing no amide bond are preferable. The weight average molecular weight of the polyacrylic acid is preferably from 500 to 20,000, particularly preferably from 1,500 to 15,000.

  As the water for diluting the detergent composition for CIP, there is no problem as long as the water is microbiologically and chemically clean and does not contain any harmful bacteria or compounds. Usually, tap water, groundwater, industrial water and the like are used. Used. Examples of the method of cleaning water used for dilution include aeration, reverse osmosis membrane filtration, ultrafiltration, diatomaceous earth filtration, ion exchange, and distillation. These may be used alone or in combination of two or more. Done. It is preferable to prepare the washing solution each time, but even if the washing solution or the rinsing water used once for washing is collected with little contamination, it is stored and stored again, and the components (A) to (D) are re-used at the time of use. Reuse cleaning can be performed by adding one or more of the above. The water for diluting the cleaning composition for CIP can be used after cooling or heating to 10 to 60 ° C. In the CIP cleaning method of the present invention, the CIP sanitizing cleaning solution is used at a temperature of 10 to 60 ° C, preferably at 13 to 40 ° C, more preferably at 15 to 30 ° C.

  In the method of the present invention, the CIP disinfecting washing solution may further contain, if necessary, a chelating agent other than the component (B), a surfactant, an organic salt, a dispersant, a solubilizer, a dye, a preservative, and a component other than the component (C). , A disinfectant, a defoamer, a corrosion inhibitor, a natural extract and the like can be blended within a range that does not impair the effects of the present invention.

  In the CIP cleaning method of the present invention, the cleaning liquid is sprayed by a rotating motion from a spray nozzle, a roller ball, a shower ball, or the like in the tank in the food and beverage production line, and the cleaning is performed so that the cleaning liquid uniformly contacts the inside. Do. In the piping, the cleaning is performed at a flow rate of 0.5 to 4.0 m / sec, preferably at a high speed of 1.0 to 3.0 m / sec.

  In the conventional CIP cleaning, 1. water washing; 2. alkali washing; 3. water rinse; 4. acid washing; 5. water rinse, 6. eradication; In the process of rinsing with water, some processes are omitted, the order is changed, or the same process is repeatedly performed, depending on the components of the CIP cleaning solution, the type and condition of dirt. In the method of the present invention, 1. water washing; 2. cleaning with a CIP cleaning solution; In the three steps of water rinsing, the same effect as in the above steps 1 to 7 in the conventional CIP cleaning can be obtained. Each step in the conventional CIP cleaning is performed in 5 to 60 minutes, and in the CIP cleaning method of the present invention, each step can be performed in 5 to 60 minutes, but can be performed in 10 to 40 minutes. preferable.

  The temperature in the conventional CIP cleaning is as follows. 1. water washing; 2. alkali washing; 3. water rinse; 4. acid washing; 5. water rinse, 6. eradication; Among the water rinsing steps, 6. water washing; In the water rinsing step, the water to be used may be used at a temperature of 10 ° C to 60 ° C without heating. 2. alkali washing; 3. water rinse; 4. acid washing; 5. water rinse, In the disinfection process, heating is often performed at 60 ° C. to 140 ° C., and thermal energy is required, resulting in poor efficiency. In the method of the present invention, 1. water washing; 2. cleaning with a CIP cleaning solution; Water rinsing can be carried out at 10 to 60 ° C. through each step. The step of cleaning with the CIP cleaning liquid is preferably performed at a CIP sterilization cleaning liquid temperature of 13 to 40 ° C, and more preferably at a CIP sterilization cleaning liquid temperature of 15 to 30 ° C. When the temperature of the CIP disinfecting cleaning solution is lower than 10 ° C., the cleaning properties of organic substances are inferior. When the temperature exceeds 60 ° C., the stability of chlorine is deteriorated after the addition of the component (C). In the method of the present invention, if the water to be diluted is 10 to 60 ° C., the heat energy required for heating can be reduced.

  Hereinafter, the present invention will be described specifically with reference to Examples and Comparative Examples, but the present invention is not limited thereto. The components used in the Examples and Comparative Examples are shown below. In addition, the numerical value of the combination of the example and the comparative example in the table represents mass% of the pure content.

(A) Component A-1: sodium hydroxide A-2: potassium hydroxide

(B) Component B-1: trisodium methylglycine diacetate (trade name: Trilon M powder, manufactured by BASF Japan, 98% active ingredient)
Component (B '): Comparative component of component B B-1: tetrasodium ethylenediaminetetraacetate (trade name: Disorbin NA, manufactured by Akzo Nobel)
B'-2: Trisodium glutamate diacetate (trade name: GLDA, manufactured by Akzo Nobel)
B'-3: Trisodium nitrilotriacetate (trade name: Trilon A92R, manufactured by BASF Japan)
B'-4: sodium tripolyphosphate (trade name: sodium tripolyphosphate, manufactured by Shimonoseki Mitsui Chemicals, Inc.)

(C) Component C-1: sodium hypochlorite (trade name: sodium hypochlorite, manufactured by Asahi Glass Co., available chlorine amount: 12%)
C-2: sodium dichloroisocyanurate (trade name: Highlight 60G, manufactured by Nissan Chemical Industries, available chlorine 60%)
C-3: 7.8% sodium hypochlorite, 2% sodium hydroxide, 1.5% sodium polyacrylate (weight average molecular weight 4,000) 1.5%, sodium silicate (Na ₂ O · 3SiO ₂ ) 5% aqueous solution

(D) Component D-1: sodium polyacrylate (trade name: Sokalan PA30CL Granule, manufactured by BASF Japan, active ingredient amount 45%, average molecular weight 8,000)
D-2: Sodium acrylic acid / maleic acid copolymer (trade name: Acusol 505N Granule, manufactured by Rohm and Haas Co., Ltd., active ingredient content 35%, average molecular weight 40,000)

Examples 1 to 35, Comparative Examples 1 to 14
An aqueous solution of the cleaning composition for CIP containing the components (A) and (B) shown in Tables 1 to 6 was prepared using artificial hard water having a concentration of 75 mg / L [German hardness 4.2 ° DH] in terms of calcium carbonate. The components (C) (additives for CIP) shown in the table were added to the CIP diluted washing solution diluted so as to have the dilution ratio shown in Table 1 to prepare a CIP sanitizing washing solution. Tables 1 to 6 show the mass ratio of the component (A) to the component (B) of the cleaning composition for CIP. Using this CIP disinfecting cleaning solution, a detergency test, a chlorine stability test and a disinfecting test were performed and evaluated. The results are shown in Tables 1 to 6. The ratio of each component in the table is% by mass, and the addition amount of the component (C) is an amount (g) per 100 g of the diluted cleaning solution obtained by diluting the cleaning composition for CIP. The effective chlorine content (% by mass) as a chlorine-based disinfectant was shown.

* 1: Organic stain cleaning test method:
The mass of a stainless steel piece (6 cm × 7 cm rectangle, SUS316 piece with a thickness of 1 mm) was measured, and a cola concentrate as an organic stain was applied so as to have a dry weight of 50 mg, and heated at 110 ° C. for 30 minutes. The mass to which the stain was attached was measured as a test piece. The test piece was immersed in 300 mL of the CIP sanitizing and washing solution, allowed to stand at the temperature shown in the table for 20 minutes, rinsed with ion-exchanged water, dried at 105 ° C. for 30 minutes, and weighed. The test piece before washing, and the difference in mass between the stainless steel piece before the stain adhered and the amount of stain, the washing rate was calculated from the change in the mass of the test piece before and after washing by the following formula, and the cleanability was evaluated based on the following criteria. .
Washing rate (%) = {(test specimen weight before washing−test specimen weight after washing) / (dirt adhesion amount)} × 100
Evaluation criteria for cleaning of organic stains:
：: 80% or more of cleaning rate (excellent in cleaning)
△: 70% or more and less than 80% of cleaning rate (good cleaning property)
×: less than 70% of cleaning rate (poor cleaning)
The evaluation criteria of Δ and ○ were judged to be practical.

* 2: Inorganic soil cleaning test method:
The mass of a stainless steel piece (a SUS316 stainless steel piece having a rectangular shape of 6 cm × 7 cm and a thickness of 1 mm) was measured, and calcium carbonate was applied thereto as an inorganic soil so as to have a dry weight of 50 mg, and heated at 110 ° C. for 30 minutes. The mass to which the stain was attached was measured as a test piece. The test piece was immersed in 300 mL of the CIP sanitizing and washing solution, left at the temperature shown in the table for 20 minutes, rinsed with ion-exchanged water, dried at 105 ° C. for 30 minutes, and the mass of the test piece was measured. The difference in mass between the test piece before washing and the stainless steel piece before soiling is regarded as the amount of soiling, and the washing rate is calculated from the change in the mass of the test piece before and after washing according to the following formula, and the cleaning performance is evaluated based on the following criteria. did.
Washing rate (%) = {(test specimen weight before washing−test specimen weight after washing) / (dirt adhesion amount)} × 100
Evaluation criteria for inorganic soil cleaning:
：: 80% or more of cleaning rate (excellent in cleaning)
△: 70% or more and less than 80% of cleaning rate (good cleaning property)
×: less than 70% of cleaning rate (poor cleaning)
The evaluation criteria of Δ and ○ were judged to be practical.

* 3: Polysaccharide stain cleaning test method:
A fermentation solution was prepared by mixing 84 g of malt, 1.0 g of hops, 35 g of granulated sugar, 0.1 g of dry yeast, and 900 g of distilled water. A piece of stainless steel (a SUS316 stainless steel piece having a rectangular shape of 6 cm × 7 cm and a thickness of 1 mm) was immersed in the above fermentation solution for 48 and a half hours at 25 ° C. for fermentation. The dried one was used as a test piece. The test piece was immersed in 300 mL of the CIP sterilization cleaning solution, left at the temperature shown in the table for 20 minutes, rinsed with ion-exchanged water, dried at 105 ° C. for 30 minutes, and then weighed. The mass difference between the test piece before washing and the stainless steel piece before soiling is defined as the amount of soiling, and the washing rate is calculated from the mass change of the test piece before and after washing according to the following formula, and the cleaning performance is evaluated based on the following criteria. did.
Washing rate (%) = {(test specimen weight before washing−test specimen weight after washing) / (dirt amount)} × 100
Evaluation criteria for polysaccharide stain cleanability:
：: 80% or more of cleaning rate (excellent in cleaning)
Δ: 70% or more and less than 80% of cleaning rate (good cleaning property)
×: less than 70% of cleaning rate (poor cleaning performance)
The evaluation criteria of Δ and ○ were judged to be practical.

* 4: Effective chlorine stability test Test method:
After a lapse of 30 minutes using 200 mL of the CIP sanitizing washing solution at the washing temperature shown in the table, the effective chlorine remaining rate was evaluated according to the following criteria. The effective chlorine residual ratio was determined by the following equation based on the residual chlorine-iodine titration method of JIS K0101.
Effective chlorine remaining rate (%) = (effective chlorine concentration in cleaning solution after heating) / (effective chlorine concentration in cleaning solution before heating) × 100
Evaluation of available chlorine stability:
：: Effective chlorine remaining rate is 70% or more △: Effective chlorine remaining rate is 50% or more and less than 70% ×: Effective chlorine remaining rate is less than 50%-: Measurement is impossible, and △ and ○ are regarded as practical. Judged.

* 5: Escherichia coli test strain for general bacteria (General bacteria):
Institute for Fermentation of Staphylococcus ^{aureus: Staphylococcus aureus NBRC13276 (10 8 CFU} / mL level), Pseudomonas aeruginosa were used: ^{Pseudomonas aeruginosa NBRC13736 (10 8 CFU /} mL level).
Test method:
A test solution was prepared by adding 0.1 mL of a bacterial solution obtained by culturing the test strain in an SCD broth medium to 10 mL of the CIP disinfecting and washing solution and contacting the solution for 15 minutes at the washing temperature shown in the table. 1 mL of this test solution was pour-cultured on an SCD agar medium containing 0.1% by mass of sodium thiosulfate, and then cultured at 37 ° C. for 24 hours. After completion of the culture, the number of viable bacteria was measured and evaluated according to the following criteria.
Evaluation criteria:
：: Log reduction of the test bacteria is 3 or more. Δ: Log reduction of the test bacteria is 2 or more and less than 3. ×: Log reduction of the test bacteria is 2 or less. Δ and ○ were judged to be practical.

* 6: Eradication test for spores (a) Test strain (Spores):
Bacillus subtilis (NBRC3134) was used as the spore bacterium.
(B) Preparation of spore suspension:
The test strain was spread on an SCD agar medium (Nissui Pharmaceutical), cultured at 30 ° C., and after culturing, it was confirmed by microscopic observation that spores had been sufficiently formed. 10 mL of sterilized pure water was put on the plate medium, the colonies were scraped, and the suspension was collected. The collected suspension was subjected to centrifugal washing three times at 10,000 rpm and 4 ° C. for 15 minutes, and after centrifugation, an appropriate amount of sterilized pure water was added, and 2.0 to 9.0 × 10 ⁸ CFU / mL. The number of bacteria was adjusted to about the same, and heat treatment was performed at 80 ° C. for 15 minutes in a water bath to obtain a spore suspension.
(C) Test method:
0.1 mL of the spore suspension was added to 10 mL of the CIP disinfecting washing solution to a final concentration of 1.5 to 5.0 × 10 ⁵ (CFU / mL), and contacted at the washing temperature shown in the table for 15 minutes. These were used as test liquids. 1 mL of this test solution was mixed and solidified in an SCD agar medium containing 0.1% by mass of sodium thiosulfate, and cultured at 30 ° C. for 2 days. After the cultivation, the number of viable bacteria was measured and evaluated based on the following criteria based on the difference from the initial number of bacteria.
Evaluation criteria:
：: A decrease in the number of bacteria with a log reduction of the test bacterium of 3 or more Δ: A decrease in the number of bacteria with a log reduction of the bacterium of 2 or more and less than 3 ×: A decrease in the number of germs with a log reduction of the test bacterium of less than 2 Δ and ○ were judged to be practical.

* 7: Scale adhesion inhibition test:
In a 200 mL glass bottle, 100 mL of the CIP sterilization and washing solution was added, and 0.4 g of calcium phosphate was added, followed by stirring for 20 minutes. The glass bottle was left standing at a constant temperature of 80 ° C. for 24 hours, rinsed with distilled water and dried, and then the presence or absence of scale adhesion of the glass bottle was visually determined, and evaluated according to the following criteria.
Evaluation criteria for scale inhibition:
Δ: No scale adhered Δ: Level slightly adhered, but no problem in use ×: Scale adhered, and Δ and ○ were judged to be practical.

Claims (4)

(A) an alkali metal hydroxide compound as component (B) contains a methyl glycine diacetic acid or a salt thereof as an ingredient, the mass ratio of the component (A) and component (B), (A) / (B) = 0 The effective chlorine amount prepared by adding a CIP additive containing a chlorine-based disinfectant as a component (C) to a diluted washing solution obtained by diluting a CIP detergent composition having a pH of 16 to 190 with water. Simultaneous CIP washing and disinfection washing of a food and beverage production line with a CIP disinfection washing solution containing 10 to 60 ° C containing 0.005 to 0.2% by mass and 0.001 to 0.6% by mass of the component (B). A CIP cleaning method characterized by performing.   The CIP cleaning method according to claim 1, wherein the component (A) contains at least one selected from sodium hydroxide and potassium hydroxide.   The component (C) contains at least one selected from the group consisting of alkali metal hypochlorites and / or alkaline earth metal hypochlorites and chlorinated isocyanurates as chlorine-based disinfectants. The CIP cleaning method according to claim 1 or 2, wherein Further, the cleaning composition for CIP containing the components (A) and (B) is added with a high molecular polymer as the component (D), and the mass ratio of the components (B) and (D) is (D) / The CIP cleaning method according to any one of claims 1 to 3, wherein (C) is contained such that (B) = 0.05 to 10.