JP7148116B2 - HARD SURFACE DETERGENT COMPOSITION, METHOD FOR ELIMINATING MICROORGANISMS OR BACTERIA CLAGGEMENTS, METHOD FOR PREVENTING PRODUCTION OF BACTERIA CLAGUE IN WASHING MACHINE, METHOD FOR CLEANING ARTICLES HAVING HARD SURFACES WITH WASHING MACHINE, AND DISINFECTANT - Google Patents

Description

Application of Article 30, Paragraph 2 of the Patent Law (1) July 4, 2018 Website http://meeting-jsme2018. com/abstracts/2018_JSME_10th_ASME_Program_Abstracts. Presented in pdf on page 498 (P2-145) of the 32nd Annual Meeting of the Japanese Society of Microbial Ecology (2) Presented at the 32nd Annual Meeting of the Japanese Society of Microbial Ecology on July 12, 2018

INDUSTRIAL APPLICABILITY The present invention is used in a washing machine for washing an article having a hard surface, washing the article and preventing the generation of bacterial flora agglomerates in the washing machine or eliminating the bacterial flora agglomerated in the washing machine. It relates to a hard surface cleaning agent composition. The present invention also relates to a method for decolonizing microorganisms or bacterial flora agglomerates, a method for preventing the formation of said bacterial flora agglomerates in a washer, and a method for cleaning articles having hard surfaces with a washer. The present invention further relates to disinfectants.

2. Description of the Related Art In recent years, automatic dishwashers are used in hotels, restaurants, and the like from the viewpoint of labor shortage, hygiene management, and the like. Oxygen-based bleaching agents and the like are blended in detergent compositions used in such automatic dishwashing machines for the purpose of improving detergency (for example, Patent Document 1).

Japanese translation of PCT publication No. 2017-510690

Washing machines, especially automatic dishwashers, often generate bacterial flora due to the abundant presence of moisture, temperature and nutrients. A coagulum of bacterial flora is a mass of a single or a plurality of microorganisms, and is composed of the microorganisms themselves, stains derived from food and the like, and organic matter produced by the microorganisms. Microbial coagulum is synonymous with slime, which is a collection of microorganisms. In order to efficiently prevent the occurrence of bacterial flora clumps or to eliminate the bacteria that have occurred, disinfectants and cleaning agents that have a disinfecting power (bactericidal action) against the microorganisms contained in them are required. It is useful to use the composition. However, the microorganisms contained in the bacterial flora coagulum have not been identified so far, and compounds effective for eradicating them have not been investigated.

INDUSTRIAL APPLICABILITY The present invention is used in a washing machine for washing an article having a hard surface, washing the article and preventing the generation of bacterial flora agglomerates in the washing machine or eliminating the bacterial flora agglomerated in the washing machine. To provide a hard surface detergent composition, particularly a hard surface detergent composition which is effective in preventing the occurrence of bacterial flora agglomerates containing microorganisms belonging to the genus Meiothermus and microorganisms belonging to the genus Tepidimonas, or for sterilizing the agglomerates of the flora. With the goal. The present invention also provides at least one microorganism selected from the group consisting of the genus Meiothermus and the genus Tepidimonas, or a method for sterilizing a bacterial flora coagulum containing the same, and a washing machine selected from the group consisting of the genus Meiothermus and the genus Tepidimonas. It is an object of the present invention to provide a method for preventing the formation of bacterial lawn clumps containing at least one microorganism. Another object of the present invention is to provide a sterilizing agent having a sterilizing effect against microorganisms belonging to the genus Meiothermus and microorganisms belonging to the genus Tepidimonas.

As a result of intensive studies to solve the above problems, the present inventors found that most of the bacterial flora coagulum formed in the washing machine contained microorganisms belonging to the genus Meiothermus and microorganisms belonging to the genus Tepidimonas. We found that compounds such as cationic surfactants and oxygen-based bleaching agents are effective for sterilization. It has not been reported that compounds such as cationic surfactants and oxygen bleaches have sterilizing power against these microorganisms. The inventors of the present invention completed the present invention by conducting further studies based on the above findings.

That is, the present invention relates to the following hard surface cleaning composition, disinfectant, and the like.
The hard surface cleaning composition of the present invention is selected from the group consisting of an alkali agent (B), a cationic surfactant, an amphoteric surfactant, a biguanide agent, an oxygen bleaching agent, an iodine agent, an alcohol, an aldehyde and a phenol compound. and at least one selected compound (A), which is used in a washing machine for washing an article having a hard surface, cleans the article, and prevents the formation of bacterial flora agglomeration in the washing machine, or It is characterized by sterilizing the bacterial lawn coagulum existing in the washing machine.
In the present specification, at least one compound selected from the group consisting of cationic surfactants, amphoteric surfactants, biguanide agents, oxygen bleaching agents, iodine agents, alcohols, aldehydes and phenolic compounds, compound (A ) is also called.
In this specification, the hard surface cleaning composition is also simply referred to as cleaning composition.
The cleaning composition of the present invention is used in cleaning machines for cleaning articles having hard surfaces. The detergent composition of the present invention is useful for cleaning articles having hard surfaces with a washing machine, preventing the formation of bacterial flora agglomerates in the washing machine, or eliminating the bacterial flora agglomerates present in the washing machine. be.
Decolonization of microbial or bacterial flora conglomerates includes reducing or eliminating said microbial or bacterial flora conglomerates.

In the present invention, the bacterial flora agglomerate may be a red-based or orange-based bacterial flora agglomerate. The cleansing composition of the present invention is useful for preventing the occurrence of red- or orange-colored coagulum or for eliminating bacteria.

In the present invention, the bacterial flora coagulum may contain at least one microorganism selected from the group consisting of the genus Meiothermus and the genus Tepidimonas.
The compound (A) has disinfecting activity against microorganisms belonging to the genera Meiothermus and Tepidimonas. On the other hand, when a coagulum of bacterial flora is formed in a washing machine such as an automatic dishwasher, microorganisms are protected by dirt and organic matter, and the sterilization effect of compound (A) is reduced. For sterilization of bacterial lawn clumps, it is important to remove organic substances, etc. to expose the microorganisms inside the bacterial lawn clumps so that they can easily come into contact with the disinfectant and exert its disinfecting effect. For this purpose, the detergent composition must have good detergency in addition to sterilization power. Since the detergent composition of the present invention contains the compound (A) and the alkaline agent (B), it has excellent detergency in addition to the sterilization power against the above microorganisms. Therefore, the detergent composition of the present invention is useful for preventing or eliminating bacterial flora clumps containing at least one microorganism selected from the group consisting of the genus Meiothermus and the genus Tepidimonas. For example, the cleaning agent composition of the present invention can be used in a washing machine in which there is a bacterial flora agglomerate containing the above-mentioned microorganisms, and by acting on the bacterial flora agglomerate, the bacterial flora agglomerate can be sterilized. Become. Also, by allowing the detergent composition to act on the microorganisms in the washing machine, the microorganisms can be sterilized. The detergent composition of the present invention is suitable as a detergent composition for washing machines in which the above-mentioned bacterial flora-containing agglomerates are present.

The bacterial flora coagulum may further contain at least one microorganism selected from the group consisting of Bacillus cereus species, Tepidiphilus, Licinibacillus and Diferisoma.
Since the compound (A) has a disinfecting effect against these microorganisms, the detergent composition of the present invention contains at least one selected from the group consisting of the genus Meiothermus and the genus Tepidimonas, in addition to the above It is useful for preventing or eradicating bacterial flora clumps containing at least one microorganism of The detergent composition of the present invention is suitable as a detergent composition for washing machines in which the above-mentioned bacterial flora-containing agglomerates are present.

In one aspect, the hard surface cleaner composition of the present invention is suitably used in a washing machine in which the bacterial flora agglomerate exists, for disinfecting the bacterial flora agglomerate while cleaning the article.
Since the cleaning agent composition of the present invention has detergency and sterilizing power against microorganisms, it is suitably used in a washing machine in which bacterial flora agglomerates are present. can be sterilized.

The present invention also includes the following methods for removing microorganisms or clumps of bacterial flora, methods for preventing the formation of clumps of bacterial flora, and methods for cleaning articles having hard surfaces using a washer.
A group consisting of an alkali agent (B), a cationic surfactant, an amphoteric surfactant, a biguanide agent, an oxygen bleach, an iodine agent, an alcohol, an aldehyde and a phenol compound in a washing machine for washing articles having hard surfaces. A solution containing at least one compound (A) selected from the above is allowed to act on at least one microorganism selected from the group consisting of the genus Meiothermus and the genus Tepidimonas or a bacterial flora clot containing the same. A method for sterilizing the above-mentioned microorganisms or bacterial flora agglomerates containing the same.
In a washing machine for washing an article having a hard surface, an alkali agent (B), a cationic surfactant, an amphoteric surfactant, a biguanide agent, an oxygen bleaching agent, an iodine agent, an alcohol, an aldehyde and At least one selected from the group consisting of the genus Meiothermus and the genus Tepidimonas in the washing machine, characterized by using a solution containing at least one compound (A) selected from the group consisting of phenolic compounds. A method for preventing the formation of bacterial flora agglomerates containing microorganisms.
CLAIMS 1. A method of washing an article having a hard surface with a washing machine for washing said article, said washing machine having a bacterial flora coagulum containing at least one microorganism selected from the group consisting of the genera Meiothermus and Tepidimonas. , an alkaline agent (B), and at least one compound (A ). A method for cleaning an article having a hard surface using a washing machine, characterized in that the article having a hard surface is washed with a solution containing the above.

As described above, the solution containing the compound (A) and the alkaline agent (B) has detergency and sterilization power against the microorganisms, so that the solution acts on the microorganisms or the bacterial flora coagulum containing the same. This makes it possible to eradicate the above-mentioned microorganisms or bacterial flora agglomerates containing them. In addition, by using a solution containing the compound (A) and the alkaline agent (B) for washing an article having a hard surface in a washing machine, the generation of bacterial flora coagulum containing the above-mentioned microorganisms is suppressed while washing the article. can be prevented. Furthermore, in a washing machine in which bacterial flora agglomerates containing the above-mentioned microorganisms are present, by using a solution containing the compound (A) and the alkaline agent (B) for washing an article having a hard surface, while washing the article It is possible to sterilize the above-mentioned microorganisms or bacterial lawn clumps containing them.
In the method of sterilizing microorganisms or bacterial flora agglomerates containing the microorganisms of the present invention, the method of preventing the occurrence of bacterial flora agglomerates in a washing machine, and the method of washing an article having a hard surface with a washing machine, the compound (A) and the alkaline agent (B) is preferably the hard surface cleaning composition of the present invention or an aqueous solution thereof.

The disinfectant of the present invention comprises at least one compound (A ), and is characterized by being used for sterilization of at least one microorganism selected from the group consisting of the genus Meiothermus and the genus Tepidimonas.
The above compound (A) has disinfecting activity against microorganisms belonging to the genera Meiothermus and Tepidimonas. The disinfectant of the present invention contains the compound (A) and is used as a disinfectant for at least one microorganism selected from the group consisting of the genera Meiothermus and Tepidimonas.

In one embodiment of the present invention, the disinfectant is further used to disinfect at least one microorganism selected from the group consisting of Bacillus cereus sp., Tepidiphilus, Licinibacillus and Diferisoma.
The sterilizing agent of the present invention has sterilizing activity against microorganisms belonging to the genera Bacillus cereus, Tepidiphilus, Licinibacillus and Diferisoma, and is also useful for sterilizing these microorganisms.

INDUSTRIAL APPLICABILITY According to the present invention, it is used in a washing machine for washing an article having a hard surface, washing the article and preventing the generation of bacterial flora agglomerates in the washing machine or reducing the bacterial flora agglomerates present in the washing machine. To provide a hard surface cleaning composition for sterilization, in particular, a hard surface cleaning composition effective for preventing the occurrence of bacterial flora agglomerates containing microorganisms belonging to the genus Meiothermus and microorganisms belonging to the genus Tepidimonas or for sterilizing the agglomerates. can do. Also, at least one microorganism selected from the group consisting of the genus Meiothermus and the genus Tepidimonas or a method for sterilizing a bacterial lawn coagulum containing the same, at least one selected from the group consisting of the genus Meiothermus and the genus Tepidimonas in a washing machine It is possible to provide a method for preventing the occurrence of bacterial flora agglomerates containing microorganisms. Further, according to the present invention, it is possible to provide a sterilizing agent having a sterilizing effect against microorganisms belonging to the genus Meiothermus and microorganisms belonging to the genus Tepidimonas.

The disinfectant of the present invention comprises at least one compound (A ) and is used for sterilization of at least one microorganism selected from the group consisting of the genera Meiothermus and Tepidimonas.
Compound (A) has a sterilizing effect against microorganisms belonging to the genus Meiothermus and microorganisms belonging to the genus Tepidimonas. The disinfectant of the present invention preferably contains compound (A) as an active ingredient.

Examples of microorganisms belonging to the genus Meiothermus include Meiothermus ruber, Meiothermus taiwanensis, Meiothermus sylvanus, Meiothermus tyralophilus, Meiothermus cervereus, Meiothermus granatisius, Meiothermus rosaceus, Meiothermus rufus, and Meiothermus thymidus. Microorganisms belonging to the genus Tepigimonas include Tepigimonas taiwanensis, Tepigimonas fonticardi, Tepigimonas aquatica, Tepigimonas ignava, Tepigimonas thermalum, and the like. The number of microorganisms belonging to the genus Meiothermus and the number of microorganisms belonging to the genus Tepidimonas may each be one, or two or more. In one aspect of the present invention, the microorganism of the genus Meiothermus may be Meiothermus luver, Meiothermus taiwanensis, or Meiothermus sylvanus, and the microorganism of the genus Tepigimonas may be Tepigimonas taiwanensis or Tepidimonas fonticardi. In one aspect, the disinfectant of the present invention is suitable for disinfection of at least one microorganism selected from the group consisting of Meiothermus ruber, Meiothermus taiwanensis, Meiothermus sylvanus, Tepigimonas taiwanensis, and Tepigimonas fonticardi. is.

Compound (A) is also useful for disinfecting microorganisms of the genus Bacillus cereus (Bacillus cereus), Tepidiphilus, Licinibacillus and Diferisoma. The disinfectant of the present invention can further be used to disinfect at least one microorganism selected from the group consisting of Bacillus cereus species, Tepidiphilus, Licinibacillus and Diferisoma.
Microorganisms belonging to the genus Bacillus cereus, genus Tepidiphilus, genus Licinibacillus, and genus Diferisoma may each be one species or two or more species.
Microorganisms belonging to the genus Tepidiphilus include Tepidiphilus succinatemandens, Tepidiphilus thermophilus, and the like. As microorganisms belonging to the genus Licinibacillus, Licinibacillus fusiformis, Licinibacillus boronitoerans, Licinibacillus glesolivorans, Licinibacillus macroloides, Licinibacillus maciliensis, Licinibacillus odissei, Licinibacillus parvivoronicapiens, Licinibacillus sinduriensis, Licinibacillus su Felix, Licinibacillus sylanilyticus, and the like. Microorganisms belonging to the genus Diferisoma include Diferisoma camini, Diferisoma paleocoriens, and the like.

Cationic surfactants, amphoteric surfactants, biguanide agents, oxygen-based bleaching agents, iodine agents, alcohols, aldehydes and phenolic compounds used as compound (A) may be used singly or in combination of two or more. May be combined.

Cationic surfactants include, for example, quaternary ammonium salts. As quaternary ammonium salts, didecyldimethylammonium chloride, didecyldimethylammonium methosulfate, benzalkonium chloride, trialkyl(3-trialkoxysilylpropyl)ammonium chloride, dialkylmethylpolyoxyethylammonium propionate, tetraalkyl Ammonium carbonate, dimethyldioctyl ammonium chloride, alkylhydroxyethyl ammonium chloride, tetraalkylammonium adipate and the like can be mentioned, and two or more of these may be used in combination. As the cationic surfactant, at least one selected from the group consisting of didecyldimethylammonium chloride, didecyldimethylammonium methosulfate, dimethyldioctylammonium chloride and benzalkonium chloride is preferred.

Examples of amphoteric surfactants include alkylpolyaminoethylglycine and salts thereof, and dioctyldiaminoethylglycine hydrochloride, dodecyldiaminoethylglycine hydrochloride, and tetradecyldiaminoethylglycine hydrochloride are preferred.
Biguanide agents include, for example, polyhexamethylene biguanide hydrochloride, chlorhexidine hydrochloride, chlorhexidine gluconate and the like, with polyhexamethylene biguanide hydrochloride and chlorhexidine gluconate being preferred.
As the oxygen-based bleaching agent, for example, sodium percarbonate is preferable.
Examples of iodine agents include sodium iodide, potassium iodide, povidone-iodine, IF-1000, Sunplas, diiodomethylparatolyl sulfone (trade name Amical 48) and the like, with povidone-iodine being preferred.

Alcohols include, for example, mono- to trihydric alcohols such as ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, 2-methoxyethanol, 2-ethoxyethanol, chlorobutanol and the like. Monohydric alcohols of number 2 to 5; dihydric or trihydric alcohols having 2 to 6 carbon atoms such as ethylene glycol, propylene glycol, dipropylene glycol, 1,4-butanediol, 1,6-hexanediol, glycerol, bronopol Examples include polyhydric alcohols. Among them, ethanol and 2-propanol are preferred.

Aldehydes include, for example, aldehydes such as formaldehyde, acetaldehyde, and glutaraldehyde, with glutaraldehyde being preferred.
Examples of the phenol compound include phenol, isopropylmethylphenol (Biosol (registered trademark), Osaka Kasei Co., Ltd.), orthophenylphenol, sodium orthophenylphenol, etc., and isopropylmethylphenol is preferred.

The disinfectant of the present invention may contain any component as long as the effects of the present invention are exhibited. Examples of optional components include solvents, stabilizers, chelating agents, builders, corrosion inhibitors, enzymes, etc., which will be described later. The content of compound (A) in the disinfectant is not particularly limited, and can be, for example, 0.1 to 99% by weight.
The form of the disinfectant of the present invention is not particularly limited, and may be either solid or liquid form, and can be appropriately selected depending on the form of compound (A) used as an active ingredient.
The method of sterilizing the above-mentioned microorganisms with the sterilizing agent of the present invention is not particularly limited, and the sterilizing agent may act on the above-mentioned microorganisms. As a method for causing the disinfectant to act on the microorganisms, the disinfectant or its solution may be brought into contact with the microorganisms. The disinfectant or its solution may be made into a mist, and the mist-like disinfectant or its solution may be brought into contact with the microorganisms.
The sterilizing agent of the present invention can be used as it is for sterilizing the above-mentioned microorganisms, and can also be used as a sterilizing agent for the above-mentioned microorganisms by blending it with a detergent composition or the like.

The hard surface cleaning composition of the present invention contains an alkaline agent (B) and the compound (A). The hard surface cleaner composition of the present invention is used in a washing machine for washing articles having a hard surface, cleans the article, and prevents the formation of bacterial flora agglomeration in the washing machine or exists in the washing machine. A detergent composition for disinfecting bacterial lawn clumps.
Since the cleaning agent composition of the present invention has detergency and sterilizing power against microorganisms, it can be used for washing the above-mentioned articles, preventing the occurrence of bacterial flora agglomerates in the washing machine, or eliminating the bacterial flora agglomerates present in the washing machine. can be used for
One kind of compound (A) and alkali agent (B) may be used, or two or more kinds thereof may be used. The cleaning agent composition of the present invention can also be blended with the disinfectant of the present invention described above.

The bacterial lawn agglomerate may be a red-based or orange-based bacterial lawn agglomerate. The cleansing composition of the present invention is useful for preventing the occurrence of red- or orange-colored coagulum or for eliminating bacteria.
An example of the flora clump includes a flora clump containing at least one microorganism selected from the group consisting of the genera Meiothermus and Tepidimonas. A lot of bacterial flora agglomerates containing at least one microorganism selected from the group consisting of the genera Meiothermus and the genera Tepidimonas tend to be red or orange. The detergent composition of the present invention has disinfecting power against microorganisms belonging to the genus Meiothermus and genus Tepidimonas, and is effective for coagulating bacterial flora containing at least one kind of microorganism selected from the group consisting of the genus Meiothermus and the genus Tepidimonas. It is suitably used for prevention of outbreak or sterilization.
In one aspect, the detergent composition of the present invention is used in a washing machine in which a bacterial flora coagulum containing at least one microorganism selected from the group consisting of the genera Meiothermus and Tepidimonas is present, and provides high detergency and Demonstrates sterilization power against bacterial flora agglomerates.

The bacterial flora coagulum may contain at least one microorganism selected from the group consisting of Bacillus cereus species, Tepidiphilus, Licinibacillus and Diferisoma. The detergent composition of the present invention also has sterilizing power against these microorganisms.
The detergent composition of the present invention includes at least one species selected from the group consisting of the genera Meiothermus and Tepidimonas, and at least one species selected from the group consisting of the genera Bacillus cereus, the genus Tepidiphilus, the genus Licinibacillus, and the genus Diferisoma. It is suitably used for preventing or eradicating bacterial flora agglomerates containing microorganisms. Examples of microorganisms belonging to the genera Meiothermus, Tepidimonas, Bacillus cereus, Tepidiphilus, Licinibacillus, and Diferisoma include the microorganisms described above.
The presence of the above-mentioned microorganisms in the bacterial flora clump can be confirmed, for example, by DNA sequence analysis of 16S rDNA of the microbial group forming the bacterial flora clump. DNA sequence analysis of 16S rDNA can be performed by the method described in Examples.

As the compound (A), the same compound (A) as in the disinfectant can be used. The cleaning agent composition of the present invention can also be blended with the disinfectant described above.

The content of the compound (A) is preferably 0.1 to 15% by weight in the detergent composition. When the content of compound (A) is within the above range, detergency and sterilization power are improved. From the viewpoint of detergency and sterilization power, the content of compound (A) in the detergent composition is more preferably 0.5 to 12%, more preferably 1 to 10% by weight. When compound (A) contains two or more compounds, the above content is defined as the total content of each compound.
In the present specification, the content (% by weight) of each component of the cleaning composition is the content of the pure component in the cleaning composition unless otherwise specified. The same applies to disinfectants.

As the alkali agent (B), any water-soluble alkali agent can be used, and for example, an alkali metal salt or an alkaline earth metal salt can be used. The alkali metal salt is preferably one or more compounds selected from the group consisting of alkali metal salts of silicic acid, alkali metal hydroxides, alkali metal carbonates and alkali metal phosphates. Sodium (sodium metasilicate, sodium sesquisilicate, sodium orthosilicate, sodium silicate No. 1, sodium silicate No. 2, sodium silicate No. 3, sodium silicate No. 4), potassium silicate (potassium metasilicate, potassium sesquisilicate , potassium orthosilicate); alkali metal hydroxides such as sodium hydroxide and potassium hydroxide; alkali metal carbonates such as sodium carbonate, potassium carbonate, sodium hydrogen carbonate and potassium hydrogen carbonate alkali metal phosphates such as trisodium phosphate are preferred. These alkaline agents may be hydrates. Among them, alkali metal salts of silicic acid, alkali metal hydroxides, and alkali metal carbonates are more preferable from the viewpoint of improving detergency and disinfecting power, and alkali metal salts of silicic acid and alkali metal hydroxides are more preferable. More preferred are sodium silicate, potassium silicate, sodium hydroxide and potassium hydroxide. Compounds exemplified as chelating agents described later are not included in the alkali agent (B) in the present invention.

The content of the alkali agent (B) is preferably 1 to 60% by weight in the detergent composition. If the content of the alkaline agent (B) is less than 1% by weight, the detergency may be insufficient, and if it exceeds 60% by weight, the article having a hard surface to be washed may be easily deteriorated. There is From the viewpoint of improving detergency, etc., the content of the alkaline agent (B) in the detergent composition is more preferably 3 to 55% by weight, more preferably 10 to 50% by weight. When two or more alkaline agents are contained, the above content is defined as the total content of each alkaline agent.

The detergent composition of the present invention preferably has a pH of 8 or higher as a 0.1% by weight aqueous solution. When the pH of the 0.1% by weight aqueous solution of the detergent composition is 8 or more, the detergency is improved. From the viewpoint of improving detergency, the detergent composition preferably has a pH of 9 or more in a 0.1% by weight aqueous solution, more preferably 10 or more, and particularly preferably 10.5 or more. From the viewpoint of improving the sterilization power, the pH of the 0.1% by weight aqueous solution is preferably 13 or less, more preferably 12.6 or less. In one embodiment, from the viewpoint of improving detergency and sterilization power, the detergent composition of the present invention has a pH of 0.1% by weight aqueous solution of preferably 8 to 13, more preferably 9 to 13, more preferably 10 to 12. 6 is more preferred, and 10.5 to 12.6 are particularly preferred.

The detergent composition of the present invention preferably further contains a chelating agent. When the detergent composition contains a chelating agent, the detergency is improved. Although the type of chelating agent is not particularly limited, chelating agents such as aminocarboxylic acid-based, phosphonic acid-based, phosphoric acid-based, and ether carboxylate-based chelating agents may be used in combination. Compounds exemplified as acid agents and compounds exemplified as builders described later are not included in the chelating agent in the present invention.

Aminocarboxylic acid-based chelating agents include ethylenediaminetetraacetic acid (EDTA), nitrilotriacetic acid (NTA), methylglycinediacetic acid (MGDA), diethylenetriaminepentaacetic acid (DTPA), hydroxyethylethylenediaminetriacetic acid (HEDTA), triethylene Tetraminehexaacetic acid (TTHA), glutamic diacetic acid (GLDA), hydroxyethyliminodiacetic acid (HIDA), dihydroxyethylglycine (DHEG), 1,3-propanediaminetetraacetic acid (PDTA), 1,3-diamino-2- Hydroxypropanehexaacetic acid (DPTA-OH), aspartic acid diacetic acid (ASDA), ethylenediaminesuccinic acid (EDDS), salts thereof, and the like.

Phosphonic acid-based chelating agents include hydroxyethylidenephosphonic acid (HEDP), nitrilotrimethylenephosphonic acid (NTMP), phosphonobutanetricarboxylic acid (PBTC), ethylenediaminetetramethylenephosphonic acid (EDTMP), salts thereof, and the like. . Phosphoric acid-based chelating agents include tripolyphosphoric acid, hexametaphosphoric acid, salts thereof, and the like.

Examples of the salt in the chelating agent include salts of alkali metals such as potassium and sodium, and salts of alkaline earth metals such as magnesium and calcium. Among them, alkali metal salts are preferable, and sodium salts or potassium salts are more preferable.
In addition, the chelating agent and the salt of the chelating agent may be mixed in the detergent composition. It may be.

As the chelating agent, aminocarboxylic acid-based chelating agents and phosphonic acid-based chelating agents are preferable from the viewpoint of improving detergency, such as ethylenediaminetetraacetic acid, nitrilotriacetic acid, methylglycinediacetic acid, ethylenediaminetetramethylenephosphonic acid, and phosphonobutane. More preferred are tricarboxylic acids and one or more of their salts.

When the detergent composition contains a chelating agent, the content of the chelating agent is preferably 5 to 60% by weight in the detergent composition. If the content of the chelating agent is less than 5% by weight, the detergency-enhancing effect resulting from the addition of the chelating agent will be insufficient. When the chelating agent exceeds 60% by weight, the metal tends to be eluted. For this reason, for example, there is a possibility that the washing machine and the objects to be washed are likely to deteriorate. The content of the chelating agent is preferably 10 to 50% by weight, more preferably 15 to 45% by weight, from the viewpoint of improving detergency. When two or more chelating agents are contained, the above content is defined as the total content of each chelating agent.

The detergent composition of the present invention may contain components other than the above components as long as the effects of the present invention are not impaired. For example, the detergent composition of the present invention contains one or more of anionic surfactants, nonionic surfactants, acid agents, builders, corrosion inhibitors, enzymes, scrubbing agents, fragrances, deodorants, and the like. may contain Each of these may be used alone, or two or more thereof may be used in combination.

The detergent composition of the present invention preferably contains at least one selected from the group consisting of anionic surfactants and nonionic surfactants. Detergency is improved when the detergent composition contains at least one selected from the group consisting of anionic surfactants and nonionic surfactants. It preferably contains a nonionic surfactant. Examples of nonionic surfactants include polyoxyalkylene dialkyl ethers, pluronic block polymers, reverse pluronic block polymers, tetronic block polymers, reverse tetronic block polymers, polyoxyalkylene alkylphenyl ethers, fatty acid alkanolamides, Polyoxyalkylene fatty acid alkanolamides, polyoxyalkylene fatty acid esters, polyoxyalkylene sorbitan fatty acid esters, polyglycerin fatty acid esters, sorbitan fatty acid esters, sucrose fatty acid esters, polyoxyethylene methyl ether fatty acid esters, polyoxyalkylene alkylamines, etc. be done.

When the detergent composition contains at least one selected from the group consisting of anionic surfactants and nonionic surfactants, the content of the surfactant is Among them, 0.01 to 10% by weight is preferable, and 1 to 8% by weight is more preferable. When two or more surfactants selected from the group consisting of anionic surfactants and nonionic surfactants are included, the above content is defined as the total content of each surfactant.

It is preferable that the detergent composition contains an acid agent, since the disinfecting power is improved. Acid agents include citric acid, succinic acid, lactic acid, formic acid, oxalic acid, acetic acid, propionic acid, malonic acid, glutaric acid, maleic acid, fumaric acid, phthalic acid, glycolic acid, salicylic acid, tartaric acid, malic acid, hydrochloric acid, Sulfuric acid, nitric acid, sulfamic acid and the like can be mentioned. When the cleaning composition contains an acid agent, the content of the acid agent in the cleaning composition is preferably 0.01 to 10% by weight, more preferably 2 to 8% by weight. When two or more kinds of acid agents are contained, the above content is defined as the total content of each acid agent.

Builders include, for example, polymeric dispersants. It is preferable to contain a polymer dispersant because the detergency is improved. As the polymeric dispersant, for example, a polymeric dispersant having a weight average molecular weight of 3,000 to 300,000 is preferred.
Examples of the polymer dispersant include polyacrylic acid, polyaconitic acid, polyitaconic acid, polycitraconic acid, polyfumaric acid, polymaleic acid, polymethaconic acid, poly-α-hydroxyacrylic acid, polyvinylphosphonic acid, and sulfonated polymaleic acid. , maleic acid-olefin copolymer, maleic anhydride diisobutylene copolymer, maleic anhydride styrene copolymer, maleic anhydride methyl vinyl ether copolymer, maleic anhydride ethylene copolymer, maleic anhydride ethylene cross-linked copolymer Polymer, maleic anhydride acrylic acid copolymer, maleic anhydride vinyl acetate copolymer, maleic anhydride acrylonitrile copolymer, maleic anhydride acrylic acid ester copolymer, maleic anhydride butadiene copolymer, maleic anhydride Isoprene copolymer, poly-β-ketocarboxylic acid derived from maleic anhydride and carbon monoxide, itaconic acid, ethylene copolymer, itaconic aconitic acid copolymer, itaconic maleic acid copolymer, acrylic itaconic acid acid copolymers, methylene malonate copolymers, itaconic acid fumaric acid copolymers, ethylene glycol ethylene terephthalate copolymers, vinylpyrrolidone-vinyl acetate copolymers, and metal salts thereof.
Examples of metal salts include salts of alkali metals such as potassium and sodium; salts of alkaline earth metals such as magnesium and calcium. Among them, alkali metal salts are preferable, and sodium salts or potassium salts are more preferable.
As the polymeric dispersant in the present invention, polyacrylates, maleic acid-olefin copolymers or salts thereof are preferable, and sodium polyacrylate, maleic acid-olefin copolymers or sodium salts thereof are more preferable.
The weight-average molecular weight is determined by gel permeation chromatography using a phosphate buffer solution and acetonitrile as developing solvents and using polyacrylic acid as a standard substance.

When the detergent composition contains a builder, its content is preferably 2 to 20% by weight, more preferably 3 to 20% by weight, in the detergent composition. When two or more types of builders are included, the above content is defined as the sum of them.

When the detergent composition contains a corrosion inhibitor, the anti-corrosion action (anti-corrosion action) of the detergent composition is improved. As the corrosion inhibitor, for example, at least one selected from the group consisting of triazole or its derivatives, benzimidazole or its derivatives, and benzothiazole or its derivatives is preferred.

Examples of triazole or derivatives thereof include triazole, benzotriazole, tolyltriazole, 5-dodecylbenzotriazole, 5-octylbenzotriazole, 5-hexylbenzotriazole, 5-butylbenzotriazole, 5-methylbenzotriazole, carboxybenzotriazole ( 4-carboxybenzotriazole, 5-carboxybenzotriazole), carboxybenzotriazole methyl ester, carboxybenzotriazole butyl ester, carboxybenzotriazole hexyl ester, benzotriazole octyl ester, dicarboxypropylbenzotriazole and the like. Benzimidazole or derivatives thereof such as benzimidazole, 2-hydroxybenzimidazole, 1-isopropenylbenzimidazol-2-one, 1-(2-propenyl)benzimidazol-2-one, 2-methylbenzimidazole, 2 - mercaptobenzimidazole, thiazolylbenzimidazole, thiabendazole and the like. Examples of benzothiazole or derivatives thereof include benzothiazole, 2-methylbenzothiazole, 2-aminobenzothiazole, 2-mercaptobenzothiazole and the like.

When the cleaning composition contains a corrosion inhibitor, the content of the corrosion inhibitor is preferably 0.001% by weight or more in the cleaning composition from the viewpoint of corrosion inhibition, and the odor peculiar to the corrosion inhibitor is eliminated. 5% by weight or less is preferable from the viewpoint of increasing the influence. The content of the corrosion inhibitor is preferably 0.001 to 5% by weight, more preferably 0.1 to 2% by weight in the detergent composition. When two or more corrosion inhibitors are contained, the above content is defined as the total content of each corrosion inhibitor.

Detergency is improved when the detergent composition contains an enzyme. Enzymes include, for example, proteases, esterases, lipases, nucleases, cellulases, amylases and pectinases. If these enzymes are included, the detergency will be improved due to the function of the enzymes. Among them, protease, amylase and lipase are preferred.

When the detergent composition contains an enzyme, the content of the enzyme is preferably 0.1 to 15% by weight, more preferably 0.5 to 10% by weight, in terms of enzyme protein weight in the detergent composition. When two or more enzymes are contained, the above content is determined as the total content of each enzyme.

The detergent composition of the present invention may optionally contain other components such as solvents, bulking agents (bulking agents), antifoaming agents and solubilizers. These may be used alone or in combination of two or more.
Water etc. are mentioned as said solvent. Examples of the bulking agent include sodium sulfate (mirabilite) and the like.
Examples of the antifoaming agent include end-capped nonionic surfactants, silicone oil, polydimethylsiloxane, and the like.
Examples of the solubilizer include caprylic acid, octylic acid, butyl polyglucoside, and the like.

The dosage form of the detergent composition of the present invention may be solid (powder, granule (particulate), tablet, tablet, flake, block, etc.) or liquid. Preferably, it is solid. The cleaning composition of the present invention is also suitably used for cartridge cleaning agents and the like.

The method for producing the detergent composition of the present invention is not particularly limited, and may be appropriately selected according to the dosage form. For example, it can be produced by mixing or stirring components to be blended in the detergent composition. There are no particular restrictions on the order in which the components are mixed, the method of mixing or stirring, and the like.

The detergent composition of the present invention is suitably used for surface cleaning of hard materials such as ceramics, glass, metals (eg, aluminum, stainless steel, silver), melamine, Tritan, plastics, and lacquerware. Articles having hard surfaces include tableware, cutlery, cooking utensils, hardware, jigs, containers, trays, and the like. Among them, articles having hard surfaces that may come into contact with food are preferred, and articles having hard surfaces such as tableware, cutlery, cooking utensils, containers, and trays are more preferred. The detergent composition of the present invention is suitable as a detergent composition for washing machines used for washing such articles.

The washing machine for washing articles having hard surfaces in which the detergent composition of the present invention is used is not particularly limited, but an automatic washing machine used for washing the articles is preferable, for example, an automatic spray washing machine. mentioned. Examples of automatic spray washing machines include automatic dishwashers and container washing machines. Among them, the detergent composition of the present invention is preferably used as a detergent composition for automatic dishwashers. In addition, the detergent composition of the present invention is preferably used for commercial or household automatic washing machines, and is more suitable for commercial automatic washing machines. It is particularly suitable as an agent composition. In one aspect, the cleaning composition of the present invention is suitably used in cleaning machines in which bacterial flora agglomerates containing the microorganisms described above are present.

In one aspect, the detergent composition of the present invention can be used for preventing or eliminating bacterial flora clumps containing at least one microorganism selected from the group consisting of the genera Meiothermus and Tepidimonas. In one embodiment, the detergent composition of the present invention is used as a hard surface detergent composition for automatic washing machines for removing bacterial lawn clots containing the above-mentioned microorganisms. It is preferably used for sterilization of clots. Also, the detergent composition of the present invention is suitably used for sterilization of the above microorganisms in automatic washing machines. The detergent composition of the present invention is also suitably used as a detergent composition for removing accumulated dirt. Accumulated dirt is dirt accumulated on an article having a hard surface, which is an object to be washed, and examples thereof include composite dirt in which components such as oils and fats, proteins, and starches are mixed.

In one aspect of the present invention, the detergent composition of the present invention comprises at least one member selected from the group consisting of the genera Meiothermus and Tepidimonas, as well as the genera Bacillus cereus, the genus Tepidiphilus, the genus Licinibacillus, and the genus Diferisoma. It can be used for preventing or eradicating bacterial lawn clumps containing at least one microorganism selected from the group. In one aspect, it is preferably used in a washing machine in which a bacterial flora agglomeration containing the above-mentioned microorganisms exists, and at least one species selected from the group consisting of the genera Meiothermus and the genera Tepidimonas, as well as Bacillus cereus species, Tepidiphilus, and Licinibacillus It can be used as a detergent composition for sterilizing at least one microorganism selected from the group consisting of the genus and genus Diferisoma, or for sterilizing agglomerates of bacterial lawns containing the above-mentioned microorganisms.
In one aspect, the cleaning compositions of the present invention are also useful for disinfecting microorganisms, such as Escherichia species bacteria, in addition to the microorganisms described above.

When using the detergent composition of the present invention to wash an article having a hard surface such as tableware (also referred to as an object to be washed) with a washing machine, the detergent composition was diluted with water in the washing machine. Alternatively, an aqueous detergent solution dissolved in water may be brought into contact with the object to be washed. By bringing the aqueous detergent solution and the object to be washed into contact, the above microorganisms can be eliminated from the object to be washed. In the case of using the detergent composition of the present invention to sterilize bacterial flora in a washing machine, the detergent composition may be used as it is, or may be diluted with water or dissolved in water. It is sufficient to allow the aqueous solution of the cleaning agent to act on the coagulum of the bacterial flora. In addition, in a washing machine in which bacterial flora agglomerates containing the above-mentioned microorganisms exist, when the above-mentioned microorganisms are to be sterilized, for example, the washing agent aqueous solution may be circulated in the washing machine. Usually, by washing the object to be washed with the aqueous detergent solution in a washing machine, the aqueous detergent solution can act on the microorganisms and bacterial flora in the washing machine. Bacteria clumps can be disinfected. The detergent composition of the present invention is suitably used for sterilizing the above-mentioned microorganisms or the above-mentioned bacterial flora agglomerates while washing objects to be washed. In addition, by washing the objects to be washed with the aqueous detergent solution in the washing machine, it is possible to prevent the occurrence of bacterial flora agglomeration in the washing machine. From the viewpoint of detergency and sterilization power, the concentration of the detergent composition in the aqueous detergent solution is preferably 0.01 to 0.7% by weight, more preferably 0.03 to 0.5% by weight.

Next, the method for sterilizing microorganisms or bacterial flora agglomerates of the present invention and the method for washing an article having a hard surface using the washing machine of the present invention will be described.
The method for sterilizing microorganisms or bacterial flora clumps of the present invention is a method for sterilizing at least one microorganism selected from the group consisting of the genera Meiothermus and Tepidimonas or bacterial flora clumps containing the same. In the method for sterilizing microorganisms or bacterial flora agglomerates of the present invention, an alkaline agent (B), a cationic surfactant, an amphoteric surfactant, a biguanide agent, and an oxygen-based A solution containing at least one compound (A) selected from the group consisting of bleaching agents, iodine agents, alcohols, aldehydes and phenolic compounds, at least one microorganism selected from the group consisting of the genus Meiothermus and the genus Tepidimonas Alternatively, it is characterized by acting on a bacterial flora agglomerate containing this.
A method for cleaning an article having a hard surface with a cleaning machine of the present invention is a method for cleaning an article having a hard surface with a cleaning machine for cleaning an article having a hard surface, comprising at least one selected from the group consisting of the genus Meiothermus and the genus Tepidimonas. In a washing machine where there is a bacterial flora coagulum containing one type of microorganism, an alkaline agent (B), a cationic surfactant, an amphoteric surfactant, a biguanide agent, an oxygen bleaching agent, an iodine agent, an alcohol, an aldehyde and An article having a hard surface is washed with a solution containing at least one compound (A) selected from the group consisting of phenol compounds.
The inventive method for disinfecting microorganisms or bacterial flora agglomerates and the inventive method for washing articles having hard surfaces with a washing machine are also collectively referred to below as the inventive method. The method of the present invention is suitably applied to washing machines in which bacterial flora agglomerates containing the microorganisms described above are present. A washing machine for washing articles having hard surfaces and preferred embodiments thereof are the same as described above. INDUSTRIAL APPLICABILITY The method for removing microorganisms or clumps of bacterial flora of the present invention is suitable as a method for removing microorganisms or clumps of bacterial flora in automatic washing machines. The method for washing an article having a hard surface using the washing machine of the present invention is suitable as a method for washing an article having a hard surface using an automatic washing machine. Compound (A) and preferred aspects thereof are the same as in the case of the disinfectant described above.

The microorganisms belonging to the genus Meiothermus and the microorganisms belonging to the genus Tepidimonas contained in the bacterial flora coagulum are the same as those described above. The flora clump may comprise at least one microorganism selected from the group consisting of Bacillus cereus species, Tepidiphilus, Licinibacillus and Diferisoma.
The compound (A) and the alkaline agent (B) and preferred aspects thereof are the same as those of the cleaning composition described above. A solution containing the compound (A) and the alkaline agent (B) is also referred to as a sterilizing cleaning solution.

As a solution containing the compound (A) and the alkaline agent (B) used in the method of the present invention (disinfecting cleaning solution), a detergent composition containing the compound (A) and the alkaline agent (B) or a solution containing the compound (A) and the alkaline agent (B) with water Aqueous solution of detergent diluted or dissolved in water; detergent composition containing alkaline agent (B) but not containing compound (A) and separately supplied compound (A) diluted with water or dissolved in water An aqueous detergent solution can be used. As the cleaning composition containing the compound (A) and the alkaline agent (B), the cleaning composition of the present invention described above is preferable. Agent compositions or aqueous solutions thereof are preferred.
Examples of the detergent composition containing no compound (A) include detergent compositions produced without compounding the compound (A) in the detergent composition of the present invention described above. The disinfectant described above can also be used for the separately supplied compound (A).
The method of supplying the detergent composition and the optionally used compound (A) is not particularly limited. In addition, when a cleaning composition not containing compound (A) and compound (A) supplied separately are used, a cartridge containing the cleaning composition and compound (A) in a separated state Detergents are also preferably used.

The concentration of the compound (A) in the sterilization cleaning solution is preferably 1 ppm or more, more preferably 50 ppm or more, from the viewpoint of being able to exhibit the above effects more sufficiently. Moreover, from the viewpoint of the influence on the wastewater treatment facility, the concentration of the compound (A) in the disinfecting cleaning liquid is preferably 10000 ppm or less, more preferably 5000 ppm or less. The concentration of compound (A) is the total concentration of the content of each compound when compound (A) contains two or more compounds. From the viewpoint of improving detergency, the pH of the sterilizing cleaning solution is preferably 8 or higher, more preferably 9 or higher, even more preferably 10 or higher, and particularly preferably 10.5 or higher. From the viewpoint of improving the sterilization power, the pH of the sterilization cleaning liquid is preferably 13 or less, more preferably 12.6 or less. In one aspect, from the viewpoint of improving detergency and sterilization power, the pH of the sterilization cleaning liquid is preferably 8 to 13, more preferably 9 to 13, still more preferably 10 to 12.6, and 10.5 to 12.6. 6 is particularly preferred.

By allowing the disinfectant washing solution to act on at least one microorganism selected from the group consisting of the genus Meiothermus and the genus Tepidimonas, or on the bacterial flora clot containing the same, the microorganism or the bacterial flora clot is effectively treated. can be sterilized. The sterilization cleaning solution is, for example, sterilization of at least one microorganism selected from the group consisting of Bacillus cereus species, Tepidiphilus spp., Licinibacillus spp. and Diferisoma spp. Bacteria can also be suitably used. In addition, by washing an article (object to be washed) having a hard surface in the washing machine with a disinfecting washing liquid containing the compound (A) and the alkaline agent (B), it is possible to remove stains on the article and remove the above-mentioned microorganisms or bacterial flora. Eradication of clots becomes possible. According to the washing method with the washing machine of the present invention, it is also possible to remove accumulated dirt on articles having hard surfaces. Articles having hard surfaces include those described above. The sterilizing cleaning liquid may contain components blended in the cleaning composition described above within a range that does not impair the effects of the present invention.

The method of causing the disinfected cleaning solution to act on the above-mentioned microorganisms or the bacterial flora clot containing them is not particularly limited. Examples include a method of forming a mist and contacting the mist-like sterilizing cleaning liquid with the microorganisms or bacterial flora coagulum containing the same. When an article having a hard surface in a washing machine is to be washed with a disinfecting washing liquid, the article in the washing machine is brought into contact with the disinfecting washing liquid.
For example, when using an aqueous detergent solution obtained by diluting or dissolving the detergent composition of the present invention in water as a sterilizing cleaning solution, from the viewpoint of the sterilizing effect and cleaning effect of the above-mentioned microorganisms, the aqueous detergent solution The concentration of the detergent composition therein is preferably 0.01-0.7% by weight, more preferably 0.03-0.5% by weight.

The method for preventing bacterial flora agglomerates of the present invention prevents the occurrence of bacterial flora agglomerates containing at least one microorganism selected from the group consisting of the genus Meiothermus and the genus Tepidimonas in a washing machine for washing articles having hard surfaces. The method. In the method for preventing the occurrence of bacterial flora agglomeration of the present invention, in a washing machine for washing an article having a hard surface, an alkaline agent (B), a cationic surfactant, an amphoteric surfactant, and a biguanide agent are used to wash the article. , and at least one compound (A) selected from the group consisting of oxygen-based bleaching agents, iodine agents, alcohols, aldehydes and phenolic compounds.
The solution containing the compound (A) and the alkaline agent (B) and preferred aspects thereof are the same as those of the disinfecting cleaning solution described above. In the washing machine, by using the disinfecting washing solution for washing the articles, it is possible to prevent the generation of bacterial flora agglomerates containing the microorganisms in the washing machine.

EXAMPLES The present invention will be described below in more detail with examples, but the present invention is not limited to these examples. In the examples, "%" means "% by weight" unless otherwise specified.

<Test Example 1>
A bacterial flora coagulum was collected from the inside of an automatic washing machine in a restaurant. Microorganisms constituting the collected bacterial lawn clumps (bacteria constituting bacterial lawn clumps) were identified by the following method.
Bacterial flora clumps were collected from three dishwashers installed in a restaurant, and genomic DNA was extracted from the bacterial flora clumps using FastDNA SPIN Kit for Soil (manufactured by MP biomedicals). Using the extracted genomic DNA as a template, 16S rDNA of the bacterial group that constitutes the bacterial flora clump was amplified by PCR using a primer set of 27f and 1492r (both primer names). The amplified 16S rDNA was incorporated into pMD20-T Vector (manufactured by Takara Bio Inc.) and transformed into Escherichia coli DH5α to construct a clone library. The library consisted of 44-52 clones, identified by analyzing a partial sequence of 16S rDNA (about 500 bp upstream).
The bacterial lawn coagulum collected from the dishwasher was found to contain Meiothermus louvre and Tepidimonas taiwanensis. In addition, it was found that the bacterial flora coagulum further contained one or more of the following microorganisms.
Meiothermus sylvanus, Meiothermus taiwanensis, Tepidimonas fonticardi, Tepidiphilus succinatemandens, Bacillus cereus, Diferisoma camini, Licinibacillus fusiformis The above microorganisms are the main microorganisms contained in the coagulum of the bacterial flora, In addition to the above, microorganisms such as Escherichia coli (Escherichia coli), Staphylococcus aureus, Salmonella, lactic acid bacteria, and mold were also included.

<Example 1>
Using each of the compounds shown in Tables 1 and 2 as a sample, the disinfecting power thereof was evaluated by the following method.
<Sterilization test>
The fungus to be tested was cultured at 60° C. in a normal bouillon medium manufactured by Eiken Chemical Co., Ltd. for 24 hours to obtain a bacterial solution. Each sample (compounds shown in Tables 1 and 2 in Example 1) was diluted with deionized water to 0.1%, heated to 60°C, and mixed with the bacterial solution at a ratio of 99:1 (volume ratio). was used as a sample-added bacterial solution. After reacting for 15 seconds, the sample-added bacterial solution was neutralized with a pH 7 buffer solution containing sterilized sodium thiosulfate, and the neutralized sample-added bacterial solution was serially diluted with physiological saline. 1000 μL of this diluted solution and 30 mL of standard agar medium manufactured by Nihon Pharmaceutical Co., Ltd. were added to the plate. Colony forming units (CFU) were counted after incubating the plates at 60° C. for 48 hours. Based on the log ₁₀ CFU difference between the control and the sample (log ₁₀ CFU of the control - log ₁₀ CFU of the sample), the disinfecting power of each sample against the test bacteria was evaluated as follows. Ion-exchanged water at 60° C. was used as a control. The difference in log ₁₀ CFU between control and sample (log _{10 CFU of control minus log 10 CFU} of sample) is denoted as Δlog ₁₀ CFU.
(Evaluation criteria for sterilization power)
◎: Sterilization effect with Δlog ₁₀ CFU of 5 or more ○: Sterilization effect with Δlog ₁₀ CFU of 3 or more and less than 5 △: Sterilization effect of Δlog ₁₀ CFU of 1 or more and less than 3 ×: Δlog ₁₀ CFU is less than 1 sterilization effect of

As test bacteria, the following microorganisms were used.
Meiothermus Louver (NBRC106122)
Meiothermus taiwanensis (RP strain)
Tepisimonas fonticardi (PL 17 strain)
Tepidimonas taiwanensis (NBRC104868)
Bacillus cereus (ATCC14579)
Licinibacillus fusiformis (CL11 strain)
Tepidiphilus succinatemandens (4BON strain)
diferisoma camini (S3R1 strain)

Tables 1 and 2 show the evaluation results of the disinfecting power.

<Examples 2 to 6>
Each composition of Examples 2 to 6 was produced by blending the components shown in Table 3 in the proportions shown in the table and mixing or heating and dissolving them.

<Comparative Examples 1 to 6>
Each composition of Comparative Examples 1 to 6 was produced by blending the components shown in Table 3 in the proportions shown in the table and heating and dissolving them.
The percentage by weight of each component in Table 3 is the ratio (% by weight) of the pure content of each component to the composition.

In Table 3, the nonionic surfactant is ADEKA Pluronic TR-702 manufactured by ADEKA CORPORATION. The sodium polyacrylate is Sokalan PACL25 manufactured by BASF, and the maleic acid/olefin copolymer is Acusol 460ND manufactured by Dow Chemical.

In the same manner as in Example 1, each composition produced in Examples and Comparative Examples was used as a sample to evaluate the sterilizing power. In addition, the detergency of each composition produced in Examples and Comparative Examples was evaluated by the following method. Table 3 shows the results.
<Detergency test>
10 g of wheat flour, 70 g of egg yolk, 3 g of lard, and 35 g of beef tallow were added to 90 g of purified water and dissolved by stirring well. The resulting mixture was tested as a composite soil. 1 g of this composite stain was spread on the inside of a glass and dried at room temperature to prepare a cleaning test sample. The glasses used are made of tempered soda glass.
(Washing condition)
The detergency test was carried out in the washing process by adding the composition so that the concentration of each composition was 0.1% with respect to the amount of water in the automatic dishwashing machine as a washing liquid, and using the automatic dishwashing machine. It was carried out by washing the sample for washing test and visually evaluating its appearance.
A door-type washing machine manufactured by Hoshizaki (model number JW-650UF) was used as an automatic dishwasher, and the washing conditions were washing time 60 seconds, washing temperature 60°C, rinsing time 8 seconds, and rinsing temperature 80°C. Tap water was used as washing water.
(Evaluation criteria for detergency)
The detergency of the composition was evaluated as follows.
(double-circle): Adhesion of a dirt is not seen at all.
◯: Adhesion of dirt is hardly observed.
Δ: Light cloudy stains are observed.
x: Remaining dirt is clearly observed.

From Table 3, the compositions of Examples 2 to 6 had sterilizing power against the above microorganisms. The compositions of Examples 2 to 5 containing an alkaline agent also had good detergency.

Claims (8)

an alkaline agent (B);
at least one compound (A) selected from the group consisting of cationic surfactants, amphoteric surfactants, biguanide agents, oxygen-based bleaching agents, iodine agents, alcohols, aldehydes and phenolic compounds;
and is used in a washing machine for washing an article having a hard surface, washing said article, and containing at least one microorganism selected from the group consisting of the genera Meiothermus and Tepidimonas in the washing machine. A hard surface characterized in that it is used to prevent the occurrence of clumps or to sterilize bacterial lawn clumps containing at least one microorganism selected from the group consisting of the genera Meiothermus and Tepidimonas present in a washing machine. cleaning composition. 2. The hard surface cleaner composition according to claim 1, wherein the bacterial lawn coagulum is a red-based or orange-based bacterial lawn coagulum. 3. The hard surface cleaning composition according to claim 1 or 2 , wherein the bacterial lawn coagulum further contains at least one microorganism selected from the group consisting of Bacillus cereus sp., Tepidiphilus sp., Licinibacillus sp. and Diferisoma sp. . 4. The hard surface cleaning composition according to any one of claims 1 to 3 , which is used in a washing machine in which the bacterial flora agglomerates are present, for sterilizing the bacterial flora agglomerates while cleaning the article. thing. In a washing machine for washing items with hard surfaces,
An alkaline agent (B) and at least one compound (A) selected from the group consisting of cationic surfactants, amphoteric surfactants, biguanide agents, oxygen bleaching agents, iodine agents, alcohols, aldehydes and phenolic compounds. A solution containing and is applied to at least one microorganism selected from the group consisting of the genus Meiothermus and the genus Tepidimonas or to a bacterial flora clot containing the same , and used to sterilize the bacterial flora clot. A method for sterilizing the microorganisms or bacterial lawn agglomerates containing the microorganisms, characterized by: In a washing machine for washing an article having a hard surface, an alkali agent (B), a cationic surfactant, an amphoteric surfactant, a biguanide agent, an oxygen-based bleaching agent, an iodine agent, an alcohol, an aldehyde and A solution containing at least one compound (A) selected from the group consisting of phenolic compounds, and a bacterial lawn coagulum containing at least one microorganism selected from the group consisting of the genus Meiothermus and the genus Tepidimonas in the washing machine. A method for preventing the occurrence of bacterial flora agglomerates containing at least one microorganism selected from the group consisting of the genus Meiothermus and the genus Tepidimonas in the washing machine, characterized in that it is used to prevent the occurrence of A method of cleaning an article having a hard surface with a washer for cleaning said article, comprising:
In a washing machine in which there is a bacterial flora coagulum containing at least one microorganism selected from the group consisting of the genus Meiothermus and the genus Tepidimonas,
An alkaline agent (B) and at least one compound (A) selected from the group consisting of cationic surfactants, amphoteric surfactants, biguanide agents, oxygen bleaching agents, iodine agents, alcohols, aldehydes and phenolic compounds. A method for cleaning an article having a hard surface using a washing machine, characterized in that the article having a hard surface is washed with a solution containing and used to sterilize the bacterial lawn coagulum . Any one of Claims 5 to 7 , wherein the solution containing the alkaline agent (B) and the compound (A) is the hard surface cleaning composition according to any one of Claims 1 to 4 or an aqueous solution thereof. The method according to item 1.