JP6590795B2 - 試料分析装置 - Google Patents
試料分析装置 Download PDFInfo
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- JP6590795B2 JP6590795B2 JP2016519275A JP2016519275A JP6590795B2 JP 6590795 B2 JP6590795 B2 JP 6590795B2 JP 2016519275 A JP2016519275 A JP 2016519275A JP 2016519275 A JP2016519275 A JP 2016519275A JP 6590795 B2 JP6590795 B2 JP 6590795B2
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- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
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- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
- G01N35/00069—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides whereby the sample substrate is of the bio-disk type, i.e. having the format of an optical disk
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/025—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1065—Multiple transfer devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N37/00—Details not covered by any other group of this subclass
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/025—Align devices or objects to ensure defined positions relative to each other
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
- B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0636—Integrated biosensor, microarrays
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0803—Disc shape
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N2021/0325—Cells for testing reactions, e.g. containing reagents
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- Analytical Chemistry (AREA)
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Description
本実施形態では、ELISA法による抗原抗体反応の化学発光を利用して、対象液体としての検体のアレルギー測定を行う生化学分析装置50及びこの生化学分析装置50に用いられる分析チップ10を本発明の試料分析装置及び分析チップの一例として説明する。
本実施形態の分析チップ10は、略円板状に形成される基板20と、基板20の中央に形成され、測定対象である対象液体が注入される注入口22と、注入口22から基板20の外縁まで放射状に形成され、毛細管現象を利用して対象液体を導入させることが可能な複数のマイクロ流路23と、を備え、それぞれのマイクロ流路23には、対象液体の成分に対し、選択的に反応する複数種類の抗原30が間隔を以て複数固定化される。これにより、1つのマイクロ流路23に複数種類の抗原30が固定化されているので、必要な対象液体の量を抑制しつつ、複数項目の測定を一度に行うことができる。また、マイクロ流路23が放射状に形成されているので、遠心力を利用してマイクロ流路23に残留する対象液体をマイクロ流路23から排出することができる。本実施形態のように、測定処理の過程で複数の種類の液体を注入、排液する処理を複数回繰り返すような試料分析において、特に有効である。
生化学分析装置50は、分析チップ10を設置可能なチップホルダ53と、チップホルダ53を回転させるチップホルダ回転ユニット54と、分析チップ10の注入口22に対象液体を注入する分注ユニット90と、対象液体と複数種類の抗原30のそれぞれの反応を一括して測定可能な測定ユニット80と、を備え、チップホルダ53をチップホルダ回転ユニット54によって回転させて、マイクロ流路23に導入した対象液体を排出する。これにより、強い表面張力を有する対象液体を、マイクロ流路23を用いて分析する分析装置にあって、迅速かつ確実に対象液体の排出を行うことができる。
12 下側ハウジング(ハウジング)
13 上側ハウジング(ハウジング)
16 液体捕捉空間
17 エア連通口(連通口)
18 開口部
20 基板
22 注入口
23 マイクロ流路(流路)
30 抗原(反応物質)
50 生化学分析装置(試料分析装置)
53 チップホルダ
54 チップホルダ回転ユニット(チップホルダ回転機構)
80 測定ユニット(測定装置)
90 分注ユニット(分注機構)
92 分注ノズル
95 ピペットチップ(先端部)
100 エアノズルユニット(エア注入機構)
110 制御ユニット(制御部)
210 分析チップ
216 液体捕捉空間
220 第1基板(基板)
222 注入口
230 第2基板(基板)
255 流路
Claims (9)
- 基板と、該基板に形成され、測定対象である対象液体が注入される注入口と、該注入口に接続され、毛細管現象を利用して前記対象液体を導入させることが可能であり、前記注入口を中心として径方向外側に前記対象液体を排出する液体排出口が形成される流路と、前記流路の外縁外側を囲う液体捕捉空間と、を備え、前記流路には、前記対象液体の成分に対して選択的に反応可能な複数の反応物質が固定化される分析チップと、
前記分析チップが設置されるチップホルダと、
前記チップホルダを回転させるチップホルダ回転機構と、
前記分析チップの注入口に前記対象液体を注入する分注機構と、
前記対象液体と複数の前記反応物質のそれぞれの反応を測定可能な測定装置と、
を備え、
前記チップホルダを前記チップホルダ回転機構によって回転している状態で前記分注機構による前記対象液体の注入を行い、排液処理では、毛細管現象を利用して前記流路に導入された前記対象液体が遠心力により前記液体捕捉空間に排出される試料分析装置。 - 前記分注機構は、先細状に形成される先端部を有し、
前記先端部が、前記注入口に挿入された状態で前記分注機構による前記対象液体の注入を行う請求項1に記載の試料分析装置。 - 前記分析チップの前記注入口は、前記基板の中心に形成される請求項1又は2に記載の試料分析装置。
- 前記分析チップの前記流路は、前記注入口から前記基板の外縁まで放射状に複数形成され、それぞれの前記流路には、前記対象液体の成分に対して選択的に反応可能な複数の反応物質が固定化される請求項1から3までの何れかに記載の試料分析装置。
- 前記分析チップは、前記流路が前記注入口を囲うように形成される請求項1から3までに記載の試料分析装置。
- 前記分析チップの前記注入口にエアを注入するエア注入機構を更に備える請求項1から5までの何れかに記載の試料分析装置。
- 前記チップホルダを回転させながら前記エア注入機構による前記分析チップへのエアの注入を行う請求項6に記載の試料分析装置。
- 前記分析チップは、
前記基板を内部に配置収納するハウジングを更に備え、
前記ハウジングは、
前記基板の上面の少なくとも一部が露出する開口部と、
前記開口部の周囲であって前記基板の上面と前記ハウジングとの間に形成される連通口と、
を有し、
前記液体捕捉空間は、前記ハウジングの内部であって、前記基板の外周側に位置する請求項6又は7に記載の試料分析装置。 - 前記連通口は、前記基板の外周よりも径方向の内側に配置される請求項8に記載の試料分析装置。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2014101542 | 2014-05-15 | ||
JP2014101542 | 2014-05-15 | ||
PCT/JP2015/063692 WO2015174430A1 (ja) | 2014-05-15 | 2015-05-12 | 試料分析装置 |
Publications (2)
Publication Number | Publication Date |
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JPWO2015174430A1 JPWO2015174430A1 (ja) | 2017-04-20 |
JP6590795B2 true JP6590795B2 (ja) | 2019-10-16 |
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Application Number | Title | Priority Date | Filing Date |
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JP2016519275A Active JP6590795B2 (ja) | 2014-05-15 | 2015-05-12 | 試料分析装置 |
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Country | Link |
---|---|
US (1) | US10288635B2 (ja) |
EP (1) | EP3144681B1 (ja) |
JP (1) | JP6590795B2 (ja) |
DK (1) | DK3144681T3 (ja) |
ES (1) | ES2733524T3 (ja) |
WO (1) | WO2015174430A1 (ja) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
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JP7127512B2 (ja) * | 2018-11-28 | 2022-08-30 | 株式会社Jvcケンウッド | 光ディスク装置及び光ディスク回転位置検出方法 |
KR102171809B1 (ko) * | 2018-11-29 | 2020-10-29 | 재단법인 구미전자정보기술원 | 반송자 주입 방식을 이용한 광열화 결함 제거 장치와 방법 |
KR20210127945A (ko) * | 2019-02-20 | 2021-10-25 | 가부시키가이샤 파트너 펌 | 고상 반응 칩 및 이것을 사용한 측정 방법 |
WO2024082187A1 (zh) * | 2022-10-19 | 2024-04-25 | 深圳华大生命科学研究院 | 流体芯片移液设备及流体芯片的移液方法 |
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JPS58137733A (ja) | 1982-02-10 | 1983-08-16 | Nippon Tectron Co Ltd | 微量液体の分注方法及びその装置 |
AU1983588A (en) * | 1987-06-23 | 1989-01-19 | Norman G. Anderson | Centrifugal fast chromatograph |
US4900446A (en) * | 1987-06-23 | 1990-02-13 | Large Scale Biology | Centrifugal fast chromatograph |
JP3062347B2 (ja) | 1992-04-24 | 2000-07-10 | キヤノン株式会社 | 分析装置 |
US20040099310A1 (en) * | 2001-01-05 | 2004-05-27 | Per Andersson | Microfluidic device |
US20050221281A1 (en) | 2003-01-08 | 2005-10-06 | Ho Winston Z | Self-contained microfluidic biochip and apparatus |
TW200734641A (en) * | 2005-12-26 | 2007-09-16 | Inst Of Microchemical Technology | Microchip for immunoassay, kit for immunoassay and immunoassay method |
JP5080740B2 (ja) | 2006-01-20 | 2012-11-21 | 凸版印刷株式会社 | 反応容器 |
JP2008076275A (ja) | 2006-09-22 | 2008-04-03 | Fujifilm Corp | 分注装置 |
JP4910727B2 (ja) | 2007-01-31 | 2012-04-04 | セイコーエプソン株式会社 | 検査装置 |
US7867450B2 (en) | 2006-11-08 | 2011-01-11 | Seiko Epson Corporation | Liquid droplet ejecting head, inspection device, and method of using inspection device |
EP2583100B1 (en) * | 2010-06-17 | 2016-03-02 | Abaxis, Inc. | Rotors for immunoassays |
WO2012001972A1 (ja) * | 2010-06-30 | 2012-01-05 | 株式会社メタボスクリーン | マイクロ化学チップ、その製造方法、及びその使用方法 |
JP2014232023A (ja) * | 2013-05-28 | 2014-12-11 | シャープ株式会社 | 分析チップ |
-
2015
- 2015-05-12 JP JP2016519275A patent/JP6590795B2/ja active Active
- 2015-05-12 US US15/311,143 patent/US10288635B2/en active Active
- 2015-05-12 EP EP15792774.0A patent/EP3144681B1/en active Active
- 2015-05-12 ES ES15792774T patent/ES2733524T3/es active Active
- 2015-05-12 DK DK15792774.0T patent/DK3144681T3/da active
- 2015-05-12 WO PCT/JP2015/063692 patent/WO2015174430A1/ja active Application Filing
Also Published As
Publication number | Publication date |
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JPWO2015174430A1 (ja) | 2017-04-20 |
US10288635B2 (en) | 2019-05-14 |
EP3144681B1 (en) | 2019-04-17 |
US20170074894A1 (en) | 2017-03-16 |
DK3144681T3 (da) | 2019-07-22 |
EP3144681A1 (en) | 2017-03-22 |
WO2015174430A1 (ja) | 2015-11-19 |
EP3144681A4 (en) | 2017-12-27 |
ES2733524T3 (es) | 2019-11-29 |
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