JP6559647B2 - 非溶出試料のワンステップ核酸増幅方法 - Google Patents

非溶出試料のワンステップ核酸増幅方法 Download PDF

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JP6559647B2
JP6559647B2 JP2016502626A JP2016502626A JP6559647B2 JP 6559647 B2 JP6559647 B2 JP 6559647B2 JP 2016502626 A JP2016502626 A JP 2016502626A JP 2016502626 A JP2016502626 A JP 2016502626A JP 6559647 B2 JP6559647 B2 JP 6559647B2
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nucleic acid
dna
sample
amplification
solid support
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JP2016512045A (ja
JP2016512045A5 (https=
Inventor
ケンリック，マイケル・ケネス
エメリー，エイダン・マーク
アラン，フィオナ・エリザベス
ウェブスター，ボニー・リー
クヴァム，エリック・リーミング
ガオ，ウェイ
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ジーイー・ヘルスケア・ユーケイ・リミテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2016502626A 2013-03-15 2014-03-14 非溶出試料のワンステップ核酸増幅方法 Active JP6559647B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361788068P 2013-03-15 2013-03-15
US61/788,068 2013-03-15
PCT/US2014/027790 WO2014143714A2 (en) 2013-03-15 2014-03-14 Methods for one step nucleic acid amplification of non-eluted samples

Publications (3)

Publication Number Publication Date
JP2016512045A JP2016512045A (ja) 2016-04-25
JP2016512045A5 JP2016512045A5 (https=) 2017-04-13
JP6559647B2 true JP6559647B2 (ja) 2019-08-14

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JP2016502626A Active JP6559647B2 (ja) 2013-03-15 2014-03-14 非溶出試料のワンステップ核酸増幅方法

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US (1) US10053686B2 (https=)
EP (1) EP2971109B1 (https=)
JP (1) JP6559647B2 (https=)
WO (1) WO2014143714A2 (https=)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104769111B (zh) * 2012-11-09 2021-04-27 环球生命科学解决方案运营英国有限公司 用于非洗提样品的一步法核酸扩增的方法
US20150111214A1 (en) * 2013-10-17 2015-04-23 Life Technologies Corporation Direct quantification of unprocessed nucleic acid samples
US9212388B1 (en) * 2014-06-30 2015-12-15 Life Technologies Corporation Direct quantitative PCR absent minor groove binders
WO2016106113A1 (en) * 2014-12-23 2016-06-30 Ge Healthcare Uk Limited Methods and reagents for reverse-transcription polymerase chain reaction
GB201515355D0 (en) * 2015-08-28 2015-10-14 Ge Healthcare Ltd A method and kit for analyte detection
FI3430373T3 (fi) 2016-03-18 2023-08-18 Hummingbird Diagnostics Gmbh Rna-fraktioiden puhdistus käyttämällä hydrofilistä polymeeristä materiaalia
CN110343738A (zh) * 2019-06-17 2019-10-18 清远市公安局 基于fta卡采集和常温保存肋软骨dna的方法和工具
KR20250047414A (ko) * 2019-07-16 2025-04-03 멜리오랩스 인코포레이티드 단일-세포 기반 디지털 고해상도 용융을 위한 방법 및 장치
US20240150821A1 (en) * 2020-10-27 2024-05-09 Wallac Oy Methods of analyzing a biological sample

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5496562A (en) * 1988-10-05 1996-03-05 Flinders Technologies Pty Ltd Solid medium and method for DNA storage
US5705345A (en) * 1991-01-10 1998-01-06 Amersham International Plc Methods and kits for preparing nucleic acids using cyclodextrin
US7122304B2 (en) * 1997-05-12 2006-10-17 Whatman, Inc. Methods for the storage and synthesis of nucleic acids using a solid support
CA2319775C (en) * 1998-02-02 2009-05-05 Gentra Systems, Inc. Compositions and methods for using a lysing matrix for isolating dna
US6746841B1 (en) * 1999-04-14 2004-06-08 Whatman Inc. FTA- coated media for use as a molecular diagnostic tool
ATE514791T1 (de) * 2003-07-09 2011-07-15 Genvault Corp Raumtemperatur-eluierung der nukleinsäuren
EP2294225B8 (en) * 2008-06-30 2015-02-18 Life Technologies Corporation Method for direct amplification from crude nucleic acid samples
US20110256592A1 (en) * 2008-12-12 2011-10-20 Eurogentec S.A Use of cyclodextrins to improve the specificity, sensitivity and yield of nucleic acid amplification reactions
EP2256195A1 (de) * 2009-05-12 2010-12-01 Qiagen GmbH Nukleinsäureaufreinigungsverfahren
ES2616110T3 (es) * 2010-12-17 2017-06-09 Wisconsin Alumni Research Foundation Método de una etapa de elución de ADN a partir de muestras de sangre
GB201104607D0 (en) * 2011-03-18 2011-05-04 Ge Healthcare Ltd Arrangement for preservation of biological samples
WO2014041093A1 (en) * 2012-09-13 2014-03-20 Ge Healthcare Uk Limited Solid matrix for one step nucleic acid amplification
CN104769111B (zh) 2012-11-09 2021-04-27 环球生命科学解决方案运营英国有限公司 用于非洗提样品的一步法核酸扩增的方法

Also Published As

Publication number Publication date
JP2016512045A (ja) 2016-04-25
US20160017315A1 (en) 2016-01-21
WO2014143714A3 (en) 2014-12-04
EP2971109B1 (en) 2018-10-24
EP2971109A2 (en) 2016-01-20
WO2014143714A2 (en) 2014-09-18
US10053686B2 (en) 2018-08-21

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