JP6534183B2 - Neutral fat accumulation inhibitor, and method for improving neutral fat accumulation inhibitory activity - Google Patents

Description

The present invention, neutral fat accumulation inhibitor, to a method of improving 及 beauty neutral fat accumulation inhibiting effect.

  It is conventionally known that polyphenol derived from oolong tea or puerh tea, catechins, and caffeine suppress accumulation of neutral fat, and oolong tea or puerh tea is used as a neutral fat accumulation inhibitor.

  For example, Patent Document 1 describes that oolong tea suppresses an increase in neutral fat in blood. In addition, it is described in Non-Patent Document 1 that a decrease in adipose tissue weight was observed by intake of puerh tea.

WO 2005-077384 pamphlet

KUAN-LI KUO, et. al, “Comparative Studies on the Hypolipidemic and Growth Suppressive Effects of Oolong, Black, Pu-erh, and Green Tea Leaves in Rats”, J. Mol. Agric. Food Chem. 2005; 53 (2), p. 480-489

  When using oolong tea or puerh tea to obtain a high effect of suppressing the accumulation of neutral fat, it is necessary to ingest a large amount of oolong tea or puerh tea.

  However, when a large amount of oolong tea or puerh tea is ingested, there is a problem that the taste of oolong tea or puerh tea becomes too thick and the flavor is lowered. Therefore, there is a need for a tea that can obtain a better effect of suppressing accumulation of neutral fat in the same amount as conventional oolong tea or puerh tea.

The present invention has been made in view of the above circumstances, neutral fat accumulation inhibitor having a high neutral fat accumulation suppression effect than ingestion singly oolong tea or Pu'er tea,及 Beauty neutral fat accumulation The purpose is to provide a method for improving the suppressive action.

  The present inventors have found that the rat is excellent in the neutral fat accumulation inhibitory effect by ingesting oolong tea and puerh tea in combination, and has completed the present invention. More specifically, the present invention provides the following.

(1) Pu'er tea leaves or Pu'er tea extract of tea, and neutral fat accumulation inhibitor containing tea leaves or oolong tea extract Oolong tea.

(2) The agent for suppressing accumulation of neutral fat according to (1), wherein the tea leaf of the puar tea or the puar tea extract , and the tea leaf of the oolong tea or the oolong tea extract are heat-sterilized at 100 ° C. or higher. .

(3) the Pu'er tea extract, and at least one of the oolong tea extract, have been extracted with water, neutral fat accumulation inhibitor according to (1) or (2).

( 4 ) A method for improving one neutral fat accumulation inhibitory action by adding the other to either puerh tea or oolong tea.

According to the present invention, to provide a neutral fat accumulation inhibitor having a high neutral fat accumulation suppression effect than ingestion singly oolong tea or Pu'er tea, a method of improving the 及 beauty neutral fat accumulation inhibitory action Can.

It is a graph which shows the amount of neutral fat in the rat concerning an example, comparative examples 1 and 2, and control examples 1 and 2. It is a graph which shows the expression level of PPAR alpha in the rat concerning an example, comparative examples 1 and 2, and control example 2.

  Hereinafter, although specific embodiments of the present invention will be described in detail, the present invention is not limited to the following embodiments in any way, and may be appropriately modified and implemented within the scope of the object of the present invention. Can. In addition, although description may be suitably abbreviate | omitted about the location where description overlaps, it does not limit the summary of invention.

<Neutral fat accumulation inhibitor>
The neutral fat storage inhibitor of the present invention includes puerh tea tea leaf or puerh tea extract , and oolong tea tea leaf or oolong tea extract.

  The neutral fat accumulation inhibitor of the present invention has a higher neutral fat accumulation inhibitory effect than taking either oolong tea or puerh tea alone. The reason is presumed to be as follows.

According to the tea leaf or puar tea extract of the puerh tea of the present invention , and the tea leaf or oolong tea extract of the oolong tea, PPARα (peroxisome proliferator-responsive receptor-α) is more than ingesting either oolong tea or puerh tea alone Can promote the expression of Here, PPARα is known to promote expression of genes associated with energy consumption and oxidation of fatty acids, and in particular, in fatty acid metabolism, promotes expression of genes related to β-oxidation such as acyl-CoA oxidase, It is known to promote fat burning. Therefore, the neutral fat accumulation inhibitor of the present invention has a higher neutral fat accumulation inhibitory effect than ingesting oolong tea or puerh tea alone.

  In addition, if a large amount of oolong tea or puerh tea is ingested for the purpose of obtaining a higher neutral fat accumulation inhibitory effect, the taste derived from tea becomes too thick and the flavor is lowered. Since the neutral fat accumulation inhibitor of the present invention is superior in suppressing neutral fat accumulation compared to taking either oolong tea or puerh tea alone, it can exert a neutral fat accumulation inhibitory effect with lower amount, and oolong tea or pueal tea It is possible to suppress the decrease in flavor due to the large intake of tea. Furthermore, the neutral fat accumulation inhibitor of the present invention can suppress the unique smell of puerle tea and the bitter taste without astringency when taken in the same amount as compared with puerh tea alone. Moreover, the neutral fat accumulation inhibitor of the present invention can combine the astringency of oolong tea and the aroma of puerh tea to give an unprecedented new taste.

  The puerh tea contained in the neutral fat accumulation inhibitor of the present invention may be any of puerh tea tea leaves or puerh tea extract. Moreover, the oolong tea contained in the neutral fat accumulation inhibitor of the present invention may be either a oolong tea leaf or an oolong tea extract.

  Although the contents of puerh tea and oolong tea in the neutral fat accumulation inhibitor of the present invention are not particularly limited, the expression of PPARα can be further promoted and a high neutral fat accumulation inhibitory effect can be exhibited. Preferably, the ratio of the solid content of the component derived from puerh tea to the solid content of the component derived from poual tea in the oolong tea extract is 1: 0.1 to 10, 1: It is more preferably 0.2 to 5, still more preferably 1: 0.5 to 2, and most preferably 1: 0.8 to 1.25.

  The solid content (wt%) of total catechin in the neutral fat accumulation inhibitor of the present invention is not particularly limited, but it can promote expression of PPARα more and exert a high neutral fat accumulation inhibitory effect, The ratio of the total polyphenol to the solid content (wt%) is preferably 0.01 to 2.0, more preferably 0.1 to 1.0, and still more preferably 0.2 to 0.5. The total catechin amount in the neutral fat accumulation inhibitor of the present invention is measured by HPLC analysis.

  The solid content (wt%) of caffeine in the neutral fat accumulation inhibitor of the present invention is not particularly limited, but it can further promote the expression of PPARα and exert a high neutral fat accumulation inhibitory effect, The ratio to the solid content (wt%) of the total polyphenol is preferably 0.01 to 2.0, more preferably 0.05 to 1.0, and still more preferably 0.25 to 0.45. The amount of caffeine in the neutral fat accumulation inhibitor of the present invention is measured by HPLC analysis.

  The solid content (wt%) of tannin in the neutral fat accumulation inhibitor of the present invention is not particularly limited, but it can further promote the expression of PPARα and exert a high neutral fat accumulation inhibitory effect, so The ratio to the solid content (wt%) of polyphenol is preferably 0.2 to 2.5, more preferably 0.4 to 2.0, still more preferably 0.6 to 1.3, and 0.9 to 0.9. 1.1 is most preferred. The amount of tannin in the neutral fat accumulation inhibitor of the present invention is measured by the iron tartrate method.

  The solid content of total polyphenols in the neutral fat accumulation inhibitor of the present invention is not particularly limited, but the higher the amount, the more the expression of PPARα can be promoted and the high neutral fat accumulation inhibitory effect is exerted, 10.0 g / 100 g or more is preferable, 15.0 g / 100 g or more is more preferable, and 20.0 g / 100 g or more is more preferable. On the other hand, if the solid content of the total polyphenol is too large, the taste derived from oolong tea and puerh tea becomes too thick and the flavor decreases, so the solid content of the total polyphenol is preferably 50.0 g / 100 g or less. 40.0 g / 100 g or less is more preferable, and 30.0 g / 100 g or less is more preferable. The total amount of polyphenols in the neutral fat accumulation inhibitor of the present invention is measured by the Forin Thiocult method.

  The solid content of total catechin in the neutral fat accumulation inhibitor of the present invention is not particularly limited, but the higher the amount, the more the expression of PPARα can be promoted and the high neutral fat accumulation inhibitory effect is exerted. 1.0 g / 100 g or more is preferable, 4.0 g / 100 g or more is more preferable, and 8.0 g / 100 g or more is more preferable. On the other hand, if the solid content of total catechin is too large, the taste derived from oolong tea and puerh tea becomes too thick, and the flavor decreases, so the solid content of total catechin is preferably 25.0 g / 100 g or less. 17.0 g / 100 g or less is more preferable, and 12.0 g / 100 g or less is more preferable.

  The solid content of caffeine in the neutral fat accumulation inhibitor of the present invention is not particularly limited, but the higher the amount, the more the expression of PPARα can be promoted, and a high neutral fat accumulation inhibitory effect is exerted. 1.0 g / 100 g or more is preferable, 5.0 g / 100 g or more is more preferable, and 7.0 g / 100 g or more is more preferable. On the other hand, when the solid content of caffeine is too high, the taste derived from oolong tea and puerh tea becomes too thick and the flavor is reduced, so the solid content of caffeine is preferably 30.0 g / 100 g or less. 20.0 g / 100 g or less is more preferable, and 10.0 g / 100 g or less is more preferable.

  The solid content of tannin in the neutral fat accumulation inhibitor of the present invention is not particularly limited, but the higher the amount, the more the expression of PPARα can be promoted and the high neutral fat accumulation inhibitory effect is exerted, 10.0 g / 100 g or more is preferable, 20.0 g / 100 g or more is more preferable, and 25.0 g / 100 g or more is more preferable. On the other hand, if the solid content of tannin is too high, the taste derived from oolong tea and puerh tea becomes too thick and the flavor decreases, so the solid content of tannin is preferably 50.0 g / 100 g or less, 40 The content is preferably at most 0. 0g / 100g, and more preferably at most 30.0g / 100g.

  The shape of the tea leaf of puerh tea or the tea leaf of oolong tea may be any shape, for example, it may be cut into a square shape, a circular shape or the like. In addition, it may be a powdery tea leaf obtained by crushing the tea leaf and rolling it into particles.

  Puerh tea extract, oolong tea extract can be extracted by a conventional known method, for example, tea tea of puerch tea, tea leaf of oolong tea at 50 to 100 ° C. (preferably 60 to 97 ° C., more preferably 70) It can be extracted for 5 to 20 minutes at -95 [deg.] C, more preferably 80-93 [deg.] C.

The solvent used for extraction is not particularly limited, but when water is used, it can efficiently extract total polyphenols, total catechins, caffeine and tannins in tea , and therefore at least any of pueal tea extract and oolong tea extract It is preferred that one be extracted with water, and it is more preferred that both of them be extracted with water. The extraction solvent may be a solvent other than water, and may be, for example, ethanol, hexane, isopropyl alcohol, liquefied carbon dioxide gas, or the like.

  Moreover, you may mix | blend the conventional well-known various component with the above-mentioned extract. As such known components, for example, in addition to a flavor and an acidulant (ascorbic acid, citric anhydride and the like), a stabilizer, a thickener, an emulsifier, a pH adjuster (such as sodium bicarbonate) can be optionally used. These components may be blended in any manner, for example, before extracting the extract in a solvent, or may be blended after extracting the extract in a solvent.

In addition, tea leaf or puar tea extract of puerh tea , and tea leaf or oolong tea extract of oolong tea are generally neutralized by heating, so that the structure of polyphenols contained in each is changed, so that neutral fat is removed. There is a possibility that the storage suppression effect may change. However, the neutral fat accumulation inhibitor of the present invention is excellent in suppression of neutral fat accumulation even if it is heat-killed by containing tea leaves of puerh tea or puar tea extract , and oolong tea tea leaves or oolong tea extract. . Specifically, heat sterilization may be performed at 100 ° C. or higher, heat sterilization may be performed at 110 ° C. or higher, and heat sterilization may be performed at 120 ° C. or higher. The upper limit of the heat sterilization temperature is not particularly limited as long as the neutral fat accumulation inhibitor of the present invention exhibits a sufficient neutral fat accumulation inhibitory effect, but may be, for example, 150 ° C. or less .

The triglyceride storage inhibitor of the present invention may be used in any form, for example, the above-mentioned tea leaf or tea extract of puerh tea , and tea leaf or oolong tea extract of oolong tea as it is in neutral fat storage It may be used as a suppression. Alternatively, it may be formulated of puerh tea tea leaf or puerh tea extract , and oolong tea tea leaf or oolong tea extract. The form of the preparation may be various forms such as tablets, granules, capsules and the like, and may be in a liquid state. Alternatively, the tea extract may be powdered and lyophilized. When formulating, it may contain additives such as commonly used binders, inclusion agents, excipients, lubricants, disintegrants and wetting agents.

  Further, the administration method of the triglyceride accumulation inhibitor of the present invention is not particularly limited, and may be, for example, oral administration or parenteral administration. Since the neutral fat accumulation inhibitor of the present invention is excellent in the suppression of neutral fat accumulation even when orally administered, it is more effective in suppressing neutral fat accumulation than oral administration alone, and oral administration is preferable.

<Method to improve neutral fat accumulation inhibitory action>
The present invention includes a method of improving the neutral fat accumulation suppressing action of one of puerh tea and oolong tea by adding the other.

  Both Puerh tea and oolong tea in the present method are liquid, and for example, the respective tea extracts may be used. Alternatively, puerh tea and oolong tea may be used as a beverage, and conventionally known ingredients used as a beverage (for example, perfumes, acidulants, stabilizers, thickeners, emulsifiers, pH adjusters) Etc.) may be included.

(Example)
Tea leaves of puerh tea and tea leaves of oolong tea were mixed so as to be 1: 1 in weight of tea leaves, and extracted at 90 ° C. for 11 minutes so as to be 40 g / L. Ascorbic acid sodium 0.3g / L and sodium bicarbonate 0.15g / L were added to the extract liquid, and sterilization for 121 degreeC 20 minutes was performed. The tea extract was powdered by lyophilization to prepare a neutral fat accumulation inhibitor according to the example.

(Comparative example 1)
A neutral fat storage inhibitor according to Comparative Example 1 was prepared in the same manner as the neutral fat storage inhibitor according to the example except that only puerh tea was used.

(Comparative example 2)
A neutral fat storage inhibitor according to Comparative Example 2 was prepared in the same manner as the neutral fat storage inhibitor according to the example except that only oolong tea was used.

  The analysis results and analysis method of the freeze-dried product of neutral fat accumulation inhibitor according to Examples and Comparative Examples 1 and 2 are shown in Table 1 below.

(Administration test)
A neutral fat diet group (n = 6), a high fat diet group (n = 6), and a neutral fat according to Comparative Example 1 for a high fat diet using a three-week-old Wistar male rat after one week acclimation A group (n = 6) of the addition of 1.0% of accumulation inhibitor, a group (n = 6) of the addition of 1.0% of the neutral fat accumulation inhibitor according to Comparative Example 2 to a high fat diet, (n = 6) The food was divided into 5 groups (n = 6) of the group (n = 6) in which 1.0% of the triglyceride accumulation suppression according to the example was added to the diet, and reared for 8 weeks. In addition, all the groups which ingested the high fat diet were made to be pair-fed, and the normal fat diet group was allowed to freely ingest. After completion of breeding, the liver weight was measured, and the neutral fat weight in the liver weight was measured. Table 2 below shows the food intake and PP (polyphenol) and caffeine amounts of the feed taken into each rat during the administration period, and the weight of neutral fat in the liver is shown in FIG. In addition, Table 2, FIG. 1 and the "Example" in FIG. 2 mentioned later are the group of the rat which ingested 1.0% of the fat storage inhibitor which concerns on an Example added to the high fat diet. “Comparative example 1” indicates a group of rats ingested by adding 1.0% of the neutral fat accumulation inhibitor according to comparative example 1 to a high-fat diet, “comparative example 2” is high The group of the rat which ingested what added 1.0% of the neutral fat accumulation inhibitor which concerns on the comparative example 2 to a fat diet is shown, and "the control example 1" shows the group of the rat which normally ingested the fat. "Control Example 2" shows a group of rats fed a high fat diet.

  From FIG. 1, the rat group according to Comparative Examples 1 and 2 is not significantly different from the rat group according to Control Example 2 and the neutral fat weight, while the rat group according to the Example relates to Control Example 2 The neutral fat weight was significantly lower than that of the rat group. Thus, it was shown that the combined use of puerh tea and oolong tea can suppress the accumulation of neutral fat weight rather than ingesting each alone.

(Analysis of expression level of PPARα)
The expression level of PPARα in each group used in the above administration test was analyzed. The analysis was performed by the following method using the rat of each group after the rearing in the said administration test.

  Total RNA was extracted from the livers of rats in each group, and cDNA was prepared therefrom and used for measurement of mRNA expression level. The measurement was performed by quantitative PCR. The expression level of mRNA was determined by the relative ratio to GAPDH (glyceraldehyde 3-phosphate dehydrogenase) used as an internal standard by SYBR Green method using ABI PRISM 7000 Real-Time PCR System. The sequences of the Primers used are shown in Table 3 below.

  The analysis results of the expression level of PPARα are shown in FIG. Note that the symbols “a”, “b”, and “ab” in FIG. 2 indicate that there is a significant difference between those without common symbols, that is, “ It shows that there is a significant difference between those having the respective signs of “a” and “b”.

  As shown in FIG. 2, it was shown that the rat group according to the example had a higher expression level of PPARα than the rat group according to Comparative Examples 1 and 2 and Control Example 2. In particular, it was shown that the rat group according to the example had a significantly higher expression level of PPARα than the rat group according to comparative example 2. Thus, it was shown that the combined use of puerh tea and oolong tea increases the expression level of PPARα rather than ingesting each alone.

  PPARα is known to be a protein that promotes fat burning. Therefore, based on the results in FIG. 1 and FIG. 2, it is considered that the combined use of puerh tea and oolong tea increases the expression level of PPARα rather than ingesting each alone, and thus the triglyceride accumulation inhibitory effect is high. Be Further, as described above, since the high neutral fat accumulation inhibitory effect can be obtained by using the Poual tea and the oolong tea in combination, the neutral fat accumulation inhibitory effect can be exhibited at a lower amount than using each of them alone. Therefore, it has been shown that the combination of puerh tea and oolong tea can suppress the decrease in flavor due to the large intake of oolong tea or puerh tea in order to obtain more effective storage of neutral fat.

(sensory evaluation)
The sensory evaluation was performed about the flavor of the high-temperature heat sterilization of the neutral-fat accumulation inhibitor which concerns on an Example and comparative example 1, 2. FIG. As the evaluation criteria, "O" is "high preference", "△" is "not high taste but not tasteable" and "X" is "not taste good" Moreover, the more detailed functionality was evaluated about each. The results are shown in Table 4.

  The neutral fat accumulation inhibitor according to the example had a higher evaluation of flavor than the neutral fat accumulation inhibitor according to Comparative Example 1. Thereby, it is shown that the neutral fat accumulation inhibitor according to the example can suppress the peculiar smell of puial tea and the bitter taste without astringency when the same amount is ingested as compared with pual tea alone. It was done. In addition, it was shown that by combining the oolong tea and the puerh tea, the astringency of the oolong tea and the aroma of the puerh tea can be integrated to give an unprecedented new taste.

Claims (3)

A method for producing a neutral fat accumulation inhibitor, comprising
The neutral fat accumulation inhibitor comprises puerh tea extract and oolong tea extract, and does not include components derived from tea other than puerle tea and oolong tea .
The method for producing includes the step of heat sterilizing the extract of puerh tea and the extract of oolong tea at 100 ° C. or higher. A method for producing a neutral fat accumulation inhibitor, comprising
The neutral fat accumulation inhibitor comprises puerh tea extract and oolong tea extract, and does not include components derived from tea other than puerle tea and oolong tea.
The method according to any one of the preceding claims, wherein the method comprises producing the at least one of the puerh tea extract and / or the oolong tea extract using water. The neutral fat accumulation inhibitor is a liquid state, The method according to claim 1 or 2.

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