JP6498714B2 - Oral composition - Google Patents

Description

  The present invention relates to an oral composition containing a digestive enzyme.

  Conventionally, digestive enzymes such as protease, amylase, galactosidase and lipase in the digestive tract are used to absorb nutrients by decomposing food-derived proteins and peptides, carbohydrates such as starch, glycogen, and lactose, and lipids in the digestive tract, and unnecessary proteins. It is known to play a major role in the degradation of carbohydrates, carbohydrates, and protein activity. In recent years, the importance of increasing the activity of these enzymes has become widely known from the viewpoint of promoting biological metabolism and maintaining health.

  On the other hand, as a digestive enzyme activator for oral or percutaneous use, it has been known to use one kind selected from cornflower, banyan tree, raspberry, grapefruit, squirrel, hawthorn, jujube and kibana Dutch sennici ( (See Patent Document 1).

JP 2008-081441 A

  In recent years, oral intake of digestive enzymes themselves or compositions containing digestive enzymes has been widely performed. Most conventional oral compositions containing digestive enzymes contain inactivated digestive enzymes. Some digestive enzymes taken orally may be degraded by stomach acid. However, in general, it is believed that when an active digestive enzyme is ingested, it has a certain effect in promoting digestion and promoting metabolism. Therefore, there is an actual benefit of orally ingesting a digestive enzyme-containing composition having a high enzyme activity as compared with the case where only digestive enzyme is contained.

  However, the conventional technology for enhancing digestive enzyme activity including the protease activity promoter described in Patent Document 1 is not sufficient in its action, and is effective for digestive enzymes whose activity should be promoted. There was a problem of requiring ingredients.

  Then, this invention makes it a subject to provide the composition and agent which have a natural product origin component which has high digestive enzyme activity as an active ingredient.

  The present invention provides an oral composition containing a digestive enzyme and a superfood.

  According to the present invention, an oral composition having high digestive enzyme activity can be provided.

Hereinafter, the present invention will be described based on preferred embodiments thereof.
The oral composition of the present embodiment contains a superfood in addition to the digestive enzyme, so that the digestive enzyme activity is excellent and high. The digestive enzyme refers to an enzyme that digests food components, and may be an enzyme that can be produced by an administration subject of an oral composition, or an enzyme that cannot be produced. Digestive enzymes are not derived from superfoods. Digestive enzymes include protease, amylase, lipase, cellulase and galactosidase. It is preferable that the digestive enzyme contained in this embodiment is an active type. An active enzyme refers to an enzyme having enzyme activity. Having enzyme activity means not having completely lost activity. Inactivation of enzyme activity occurs when the enzyme protein is denatured by heating, pH change, or the like, and the three-dimensional structure of the active site is changed, so that the substrate cannot bind to the enzyme.

(Protease)
In the present specification, protease is a general term for enzymes that catalyze the hydrolysis of peptide bonds in proteins and peptides. Proteases are roughly classified into two types according to catalytic action, and those that catalyze the release of peptides by hydrolyzing peptide bonds inside molecules such as proteins and peptides are endoprostheses (proteinases). An exoprotease is a catalyst that catalyzes the liberation of an amino acid by hydrolyzing a peptide bond from an amino group terminal or a carboxyl terminal terminal.

  As the protease, it is preferable to contain cysteine protease in view of the high enzyme activity promoting effect by superfood, and it is particularly preferable to contain papain. Papain is known to be obtained, for example, as immature papaya fruit and / or fruit juice or processed products thereof. The papaya is not particularly limited as long as it is a plant of the genus Papaya known as a fruit native to tropical America, and examples thereof include Carica papaya. In the oral composition of the present embodiment, papain may be in a solid state, and in a liquid state such as a liquid, a paste, a gel, a jelly, a cream, an emulsion, a spray, a mousse, or a lotion. There may be. Examples of solids include powders, granules, and granules. As a protease such as papain, it is preferable to use a protease having a proteolytic titer of 5,000 units / g or more, more preferably 50,000 units / g or more. The proteolytic titer referred to herein is the absorbance at a wavelength of 275 nm corresponding to 1 μg of L-tyrosine in 1 minute of the initial reaction at 37 ° C. and pH 6.0 using casein (dairy) as a substrate. The activity to be increased is one unit. The proteolytic titer is sometimes referred to as protein digestive power, and can be measured by the method described in Examples described later.

(amylase)
Amylase is a general term for enzymes that convert amylose and amylopectin in starch and glycogen into glucose, which is a monosaccharide, and maltose and oligosaccharide, which are disaccharides, by hydrolyzing glycosidic bonds. Amylases include α-amylase, β-amylase, and glucoamylase.
α-Amylase, also known as 1,4-α-D-glucan glucanohydrolase or glycogenase, is an enzyme that generates polysaccharides, maltose, and oligosaccharides by randomly cleaving the α-1,4 bond of starch and glycogen. It is.
β-amylase is also called 1,4-α-D-glucan glucanomaltohydrolase, glycogenase or saccharogen amylase, which breaks down starch and glycogen into maltose.
The official name of glucoamylase is glucan 1,4-α-glucosidase, and 1,4-α-D-glucan glucohydrolase is exo 1,4-α-glucosidase, γ-amylase, lysosomal α-glucosidase or amyloglucosidase. Use an alias. It hydrolyzes the α-1,4-linkage at the non-reducing end of the sugar chain into an exo form, producing one glucose molecule. Those that also cleave α-1,6-bonds are also known.

(Lipase, cellulase, galactosidase)
Examples of the lipase include triacylglyceride lipase and phospholipase. Cellulases include endoglucanases and exoglucanases. Examples of galactosidase include β-galactosidase.

  As amylase, protease, lipase, cellulase, and / or galactosidase, those extracted from plants or bacteria containing these enzymes, synthetic products, etc. may be used. In the oral composition of the present embodiment, α-amylase may be in a solid state, and it may be a liquid, paste, gel, jelly, cream, emulsion, spray, mousse, lotion or the like. It may be a shape. Examples of solids include powders, granules, and granules.

(Super food)
Superfood refers to foods that have a good nutritional balance and high nutritional value, or foods that are prominently rich in certain nutritional and health ingredients. Super foods include honey, bee pollen, amaranth, quinoa, buckwheat, black rice, red rice, hato barley, high acne, acne, wow, fin, chia seed, wild rice, hemp, flax seed, egoma, Tigernut, Mayanut, Cacao, Sasha Inch, Almond, Walnut, Green Coffee, Freeca, Coconut, Avocado, Lekuma, Acai, Cam Come, Acerola, Makiberry, Goldenberry, Mulberry, Pomegranate, Dragon Fruit (also called “Pitaya” ), Wolfberry (sometimes called "Godberry"), aronia (sometimes called "Chalkberry"), noni, red maca, turmeric, turmeric, maca, yacon, mesquite, spirulina, chlorella, AFA Blue Green Algi , Da Vinegar, kale, broccoli sprouts, moringa, aloe vera, wheatgrass (wheat young leaves), Baobab, Kupuasu, Shibakuson, beets, such as Chaga and the like. However, when the composition of the present invention contains a cam cam, it is essential to contain a digestive enzyme other than papain. Superfoods include coconut, acai, cacao, camouflage, maquiberry, golden berry, mulberry, pomegranate, dragon fruit, aronia, noni, spirulina, chlorella, kale, broccoli sprout, moringa, aloe vera, wheatgrass, maca, amaranth, quinoa It is preferable to use at least one selected from wolfberry, chico seed, baobab and hemp because of its high effect of improving digestive enzyme activity, especially pomegranate, moringa, wolfberry, chia seed, baobab, hemp, noni, dragon fruit, maquiberry, coconut , Golden berry, cam cam, acai, maca, quinoa, cacao, spirulina, amaranth, broccoli sprout are preferably used. The oral composition of this embodiment is more preferably a combination of two or more superfoods. In addition to digestive enzymes and superfoods, it is more preferable to combine with other components such as thickeners, excipients and fats and oils.

  Coconut is a fruit of a monocotyledonous palm tree, usually a fruit of the coconut genus Cocos (scientific name Cocos nucifera). Coconuts usually have a solid endosperm layer at the inner periphery of a hard shell and liquid endosperm near the center. The coconut preferably uses endosperm, and the endosperm may be either solid endosperm or liquid endosperm, or a mixture thereof. For example, coconut milk is a juice obtained by pulverizing and pressing the endosperm portion of coconut fruit. There are also products such as coconut milk powder obtained by drying the juice by spray drying or the like.

  Acai is a plant of the palm family, the Japanese name is Wakaba cabbage palm, and the scientific name is Euterpe oleracea. As the acai, normally, the skin and / or the fruit part (hereinafter, the fruit skin and the fruit part are simply referred to as the fruit part) are preferably used.

  Cam Cam is also known as Cam Cam Berry, and is a berry-family Myrtaceae plant native to the South American rainforest, such as Myrciaria dubia. As the cam cam, a fruit part excluding seeds is particularly preferably used.

  Maki berry is a fruit of the family Aristotelia chilensis which is known for its origin in southern Chile, Patagonia, etc., for example, the fruit of Aristotelia chilensis.

  The golden berry is a fruit of a plant belonging to the genus Coleaceae native to South America, and examples thereof include a fruit of Physalis peruviana.

  Mulberry is a general term for plants belonging to the genus Mulaceae. As for mulberry, a fruit part is particularly preferably used.

  The pomegranate is a deciduous small tree of the genus Pomegranate, for example, Punica granatum. The pomegranate is preferably a fruit part, and it is particularly preferable to use edible seed coats and seeds.

  Dragon fruit is also called Pitaya, and is a fruit such as the cactus cactus belonging to the genus Cactaceae from the tropical rainforests of Mexico or Central and South America. The dragon fruit preferably uses the pulp part, and may contain seeds.

  Aronia uses the fruit of a deciduous shrub plant belonging to the genus Aronia. Aronia arbutifolia ripening in red and Aronia melanocarpa ripening in black are known as aronia, but it is preferable to use aronia melanocarpa.

  Noni is an evergreen small tree belonging to the genus Yaeyama Aoki, for example, Morinda citrifolia. Noni is sometimes called Yaeyama Aoki. As noni, it is preferable to use a leaf, a stem, and a fruit part, and it is preferable to contain a leaf especially. The leaves may contain stems and / or fruits.

  Cacao is an evergreen tree of the mallow family (Aogiri family depending on the classification system). Also called cacao tree or cocoa tree, the scientific name is Theobroma cacao. It is preferable to use seeds for cocoa.

  Spirulina is a unicellular microalga of the order Cyanobacteria, and belongs to the genus Spirulina or Arthrospira. Spirulina is generally known to have a helical shape. As spirulina, those cultured while photosynthesis are used. Spirulina usually uses the entire algal body.

  Chlorella is a general term for the freshwater single-cell green algae of the genus Chlorella. As a chlorella, what was cultured while carrying out photosynthesis is used. As the chlorella, it is preferable to use a dry ground powder of algal cells.

  Kale is a plant belonging to the genus Brassicaceae, such as Brassica oleracea var. Acephala. Kale usually uses leaves.

  Broccoli sprout is a bud of broccoli, which is a plant of the Brassicaceae genus Brassica, and normally uses a new shoot harvested within 14 days after germination. Examples of broccoli include the scientific name Brassica oleracea var. Italica.

  Moringa belongs to the Wasabiaceae family, and is a deciduous tree that grows in Southeast Asia such as Indonesia and Thailand, the Arabian Peninsula, and the tropical and subtropical regions of India, and has a tree height of up to about 10m. Moringa may use whole plants, but preferably leaves and flowers are used, particularly preferably leaves.

  Aloe vera is a kind of succulent plant belonging to the genus Aloe, also called scientific name Aloe vera. Aloe vera uses leaves or their juice. When using leaves, the outer skin may be removed and only the jelly-like mesophyll may be used, or the entire outer skin may be used.

  Wheatgrass, also called young wheat leaves, refers to buds germinated from wheat seeds. As wheatgrass, shoots harvested within 30 days from germination are usually used.

  Maca is a perennial plant of the Brassicaceae Lepidium that grows in Peru, South America, such as Lepidium meyenii. It is preferable to use roots as maca.

  Amaranth is a general term for plants of the genus Amaranthus (Amaranthus). As amaranth, it is preferable to use seeds.

  Quinoa is a plant of the genus Chenopodium belonging to the subfamily Azaza (Chenopodium), and includes Chenopodium quinoa. As quinoa, it is preferable to use seeds.

  The wolfberry is a deciduous shrub of the solanaceae native to East Asia (China to Japan), and its scientific name is called Lycium chinense. As for wolfberry, it is preferable to use a fruit part.

  Chia seeds are chia seeds. Chia is an annual plant of the genus Akigiri, and its scientific name is Salvia hispanica.

  Baobab is a generic name for the genus Adansonia (Alanaceae) (Adapted in the Cronquist system and the New Engler system). It is preferable to use a fruit part for baobab.

  Hemp is a family of Asaidaceae, and has the scientific name Cannabis sativa. It is preferable to use fruit as the hemp.

  When obtaining papain as a processed product of papaya fruit or fruit juice, and when obtaining the above superfood that is a plant or alga, the plant or alga that is a papaya or superfood is either immediately after harvest or after harvest It is preferable that it has been processed immediately. When time is required for the treatment, it is preferably stored by a storage means commonly used by those skilled in the art, such as low-temperature storage, in order to prevent deterioration of plants or alga bodies that are papayas and superfoods. Hereinafter, a plant or alga that is papaya or superfood is also referred to as a “specific plant or alga”.

  As a processed product of a specific plant body or alga body, for example, a dry powder (also referred to as dry pulverized powder) of a specific plant body or alga body, a fragmented product of a specific plant body or alga body, and a dry powder thereof, specific Squeezed juice of a plant or alga and dried powder thereof, an extract of a specific plant or alga, and dried powder thereof, but are not limited thereto. However, from the viewpoints of ease of processing, storage, transportation, etc. and versatility of use forms, finally, a dry powder (dry pulverized powder, dried powder of shredded product, dried powder of juice or dried powder of extract) It is preferable that it is the form of. For example, for papain, papaya fruit or fruit juice extract and its dry powder are preferred, for coconut, the above coconut milk powder is preferred, for acai, acai fruit dry powder (dry ground powder) or fruit juice dry powder and those Extract and its dry powder are preferable, and for cam cam, dry powder of fruit portion of cam cam or dry powder of fruit juice and extract thereof and dry powder thereof are preferable, maki berry, golden berry, mulberry, pomegranate, dragon fruit, For aronia and aloe vera, a dry pulverized powder of the fruit part or a dry powder of fruit juice, an extract thereof and a dry powder thereof are preferable. In addition, for cacao, cacao seeds as it is or a pulverized product thereof and extracts thereof and a dry powder thereof are preferable. For moringa, noni, kale, broccoli sprout, and wheatgrass, dry powder of powder (dried powder) or leaf Juice or dried powder thereof and extract thereof and dry powder thereof are preferable. For maca, dry powder of maca root (dried and ground powder) and extract and dry powder thereof are preferable. If it is amaranth, amaranth Preferred seed dry powder (dry ground powder) and its extract and dry powder are preferred. For quinoa, dry seed powder (dry ground powder) and its extract and dry powder are preferred, and wolfberry is wolfberry fruit. Dry powder (dry pulverized powder) or wolfberry juice dry powder (dry pulverized powder) Those extracts and their dry powders are preferred, chia seeds are dry powder of chia seeds and their extracts and their dry powders are preferred, and baobabs are dry ground powder of fruit of baobab or fruit juice and its extract and its extract and its A dry powder is preferred, and as for hemp, a dry powder (ground powder) of hemp seeds and an extract thereof and a dry powder thereof are preferred. Furthermore, for spirulina and chlorella, dry powder of algal bodies (dry pulverized powder), extract thereof and dry powder thereof are preferable.

  For example, a conventionally known method can be used to dry a specific plant body or algal body into a dry powder (dry pulverized powder). As such a method, a method in which a drying process and a pulverization process are combined with a specific plant body or algal body can be used. Either the drying process or the pulverization process may be performed first, but the drying process is preferably performed first. Dry powdering (dry pulverization) may be combined with one or more treatments selected from treatments such as sterilization treatment, if necessary. In addition, the number of times of pulverization may be combined with one or two or more processes, but it is preferable to combine fine pulverization that finely pulverizes after performing coarse pulverization.

  The sterilization treatment is not particularly limited as long as it is a treatment generally known to those skilled in the art. For example, the sterilization treatment is a treatment that physically or chemically kills microorganisms using temperature, pressure, electromagnetic waves, chemicals, or the like. it can. When the sterilization treatment is performed in addition to the drying treatment and the pulverization treatment, the sterilization treatment is preferably performed after the drying treatment or before or after the pulverization treatment.

  Although a drying process is not specifically limited, For example, the process dried so that the water content of a specific plant body or algal body may be 10% or less, Preferably it is 5% or less is mentioned. The drying treatment can be performed by any method known to those skilled in the art, such as hot air drying, high pressure steam drying, electromagnetic wave drying, freeze drying, and the like. Drying by heating can be performed at a temperature and a time at which a specific plant or alga does not discolor by heating at, for example, 40 ° C to 140 ° C, preferably 80 ° C to 130 ° C.

  The pulverization process is not particularly limited, and examples thereof include a process of pulverizing a plant body by an arbitrary method commonly used by those skilled in the art using a crusher, a mill, a blender, a millstone, or the like. The pulverized specific plant body or algal body is sieved as necessary. For example, it is preferable to use a powder passing through 30 to 250 mesh as the powder of the specific plant body or algal body. By making the particle size less than or equal to 250 mesh, it becomes easier to handle the powder of a specific plant or algae during further processing, and by making the particle size more than 30 mesh, Uniform mixing of the algal powder and other materials is facilitated.

  As a specific dry powdering method, for example, a specific plant or alga body is cut, dried so that the water content is 10% by mass or less, preferably 5% by mass or less, and then pulverized. Is mentioned. In addition to this, for example, a method of cutting a specific plant body or alga body, then twisting, and then drying and pulverizing; And a method of further pulverizing.

  A method for fragmenting a specific plant or alga body is not particularly limited, and for example, a method commonly used by a person skilled in the art, such as slicing, crushing, and chopping, to fragment a plant can be used. . As an example of fragmentation, a slurry may be used. Slurry is to put a specific plant or alga body in a mixer, juicer, blender, mascoloider, etc. to make a papaya and a specific plant body or alga body slender (suspension of liquid and solid). To do.

  The method for squeezing a specific plant or alga body is not particularly limited. For example, a method of squeezing a specific plant or alga body or a fragmented product thereof, or centrifugation of a specific plant or alga body fragmented product And a filtering method. As a specific example of the squeezing method, the squeezed juice is obtained by squeezing with a mechanical crushing means such as a mixer or a juicer, and if necessary, removing the crude solid content by means such as sieving or filtration. A method is mentioned.

  A method for obtaining an extract (extract) of a specific plant body or alga body is not particularly limited. For example, a specific plant body or alga body or a fragmented product or a dried product thereof is added to ethanol, water, hydrous ethanol, or the like. An extraction solvent usually used by a trader may be added, and extraction may be performed by stirring or heating as necessary. The extract may be concentrated as necessary. However, the papaya extract preferably contains a papaya-derived enzyme such as papain.

  Examples of the extraction solvent used when obtaining an extract of a specific plant or alga body include, but are not limited to, water, organic solvents, and hydrous organic solvents (hydrous alcohols such as hydrous ethanol). When water is used as a solvent, warm water or hot water may be used. As the organic solvent used for the extraction, an organic solvent that is usually allowed when extracting a natural product component is used. For example, methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, butane, Acetone, hexane, cyclohexane, propylene glycol, hydrous ethanol, hydrous propylene glycol, ethyl methyl ketone, glycerin, methyl acetate, ethyl acetate, diethyl ether, dichloromethane, edible oils and fats, 1,1,1,2-tetrafluoroethane, 1, 1,2-trichloroethene and the like can be mentioned. These solvents can be used alone or in combination of two or more. Among these solvents, water, water-containing ethanol and water-containing propylene glycol are preferably used.

  The method for extracting a specific plant or alga body is not particularly limited as long as it is an acceptable method for extracting a natural product component. For example, solid-liquid extraction such as a warm extraction method or a supercritical fluid extraction method is possible. Law.

  The warm extraction method is, for example, a method in which a test substance and a solvent are brought into contact with each other and treated at a temperature not higher than the boiling point of the solvent to extract components contained in the test substance into the solvent. A reflux extraction method may be used.

  On the other hand, the extraction may be performed at a relatively low temperature, for example, 10 ° C. or more and 50 ° C. or less, or 20 ° C. or more and 45 ° C. or less.

  The supercritical fluid extraction method is, for example, a method of performing extraction using a supercritical fluid that is a fluid that exceeds the critical point (critical temperature, critical pressure) of a gas-liquid substance. Examples of the supercritical fluid include carbon dioxide, ethylene, propane, nitrous oxide (laughing gas), and preferably carbon dioxide.

  In the supercritical fluid extraction method, an extraction process for extracting a target component with a supercritical fluid and a separation process for separating the target component and the supercritical fluid are performed. In the separation step, any one of extraction separation by pressure change, extraction separation by temperature change, and extraction separation using an adsorbent / absorbent may be performed.

  You may perform supercritical fluid extraction by the entrainer addition method. In this method, for example, ethanol, propanol, n-hexane, acetone, toluene, other aliphatic lower alcohols, aliphatic hydrocarbons, aromatic hydrocarbons, and ketones are added to the extraction fluid in an amount of 2 to 20 W / V. It is a method that drastically increases the solubility of the target extract in the extraction solvent or enhances the separation selectivity by performing supercritical fluid extraction using this fluid. An extract of a specific plant body or alga body can be obtained.

  Since the supercritical fluid extraction method can be operated at a relatively low temperature, it can be applied to substances that are altered or decomposed at high temperatures, the advantage that the extraction fluid does not remain, and the recycling of the solvent is possible. There is an advantage that the process can be omitted and the process becomes simple.

  The specific plant body or alga body extraction method may be performed by a liquid carbon dioxide batch method, a liquid carbon dioxide reflux method, a supercritical carbon dioxide reflux method, or the like, in addition to the extraction method described above. A plurality of extraction methods may be combined. By combining a plurality of extraction methods, it is possible to obtain extracts of specific plants or alga bodies having various compositions.

  Extracts of specific plants or algae obtained by extraction can be purified by ultrafiltration, column methods using adsorbent carriers (diaion HP-20, Sephadex-LH20, chitin, etc.), batch methods, etc. It is preferable from the viewpoint of safety.

  In the present invention, the specific plant body or alga body used as the active ingredient and the processed product of the specific plant body or alga body may be commercially available products, and are described, for example, in Examples described later. Things.

  The content of the digestive enzyme and the superfood in the composition and agent of the present invention may be composed of only active ingredients as long as it is an effective amount capable of at least high digestive enzyme activity. It is preferable because a higher digestive enzyme activity can be achieved. Since the composition of the present invention contains superfood, the digestive enzyme activity is enhanced as compared with the case where a specific digestive enzyme is contained alone. For example, when the composition of this embodiment contains papain, it has a higher protease activity than when it contains papain alone, and when it contains α-amylase, it contains α-amylase alone. It has a higher amylase activity than

  For example, the dry mass ratio of digestive enzyme and superfood of the composition ([digestive enzyme]: [superfood]) is preferably 1: 0.001 or more and 1: 100 or less, more preferably 1: 0.003 or more and 1 : 10 or less, particularly preferably 1: 0.005 or more and 1: 1 or less, and particularly preferably 1: 0.01 or more and 1: 0.1 or less. For example, when the composition includes a papaya extract containing papain as a digestive enzyme, the amount of the papaya extract is regarded as the amount of papain (the same applies hereinafter). When an α-amylase extract is used as a digestive enzyme, the entire α-amylase extract is considered to constitute the digestive enzyme.

  In particular, when the composition contains coconut, the dry mass ratio of digestive enzyme and coconut (digestive enzyme :: coconut]) is preferably 1: 0.001 or more and 1: 100 or less, more preferably 1: 0. 003 or more and 1:10 or less, particularly preferably 1: 0.01 or more and 1: 1 or less.

  In particular, when the composition contains acai, the dry mass ratio of digestive enzyme and acai ([digestive enzyme]: [acai]) is preferably 1: 0.001 or more and 1: 100 or less, more preferably 1: 0. 0.003 or more and 1:10 or less, and particularly preferably 1: 0.01 or more and 1: 1 or less.

  Particularly when the composition contains cacao, the dry mass ratio of digestive enzyme and cacao ([digestive enzyme]: [cacao]) is preferably 1: 0.001 or more and 1: 100 or less, more preferably 1: 0. 0.003 or more and 1:10 or less, and particularly preferably 1: 0.01 or more and 1: 1 or less.

  Particularly when the composition contains spirulina, the dry mass ratio of digestive enzyme and spirulina ([digestive enzyme]: [spirulina]) is preferably 1: 0.001 or more and 1: 100 or less, more preferably 1: 0. 0.003 or more and 1:10 or less, and particularly preferably 1: 0.01 or more and 1: 1 or less.

  Particularly when the composition contains moringa, the dry mass ratio of digestive enzyme and moringa ([digestive enzyme]: [moringa]) is preferably 1: 0.001 or more and 1: 100 or less, more preferably 1: 0. 0.003 or more and 1:10 or less, and particularly preferably 1: 0.01 or more and 1: 1 or less.

  In particular, when the composition contains maca, the dry mass ratio of digestive enzyme and maca ([digestive enzyme]: [maca]) is preferably 1: 0.001 or more and 1: 100 or less, more preferably 1: 0. 0.003 or more and 1:10 or less, and particularly preferably 1: 0.01 or more and 1: 1 or less.

  Particularly when the composition contains amaranth, the dry mass ratio of digestive enzyme and amaranth ([digestive enzyme]: [amaranthus]) is preferably 1: 0.001 or more and 1: 100 or less, more preferably 1: 0. 0.003 or more and 1:10 or less, and particularly preferably 1: 0.01 or more and 1: 1 or less.

  In particular, when the composition contains quinoa, the dry mass ratio of digestive enzyme and quinoa (digestive enzyme): [quinoa]) is preferably 1: 0.001 or more and 1: 100 or less, more preferably 1: 0. 003 or more and 1:10 or less, particularly preferably 1: 0.01 or more and 1: 1 or less.

  In particular, when the composition contains wolfberry, the dry mass ratio of digestive enzyme and wolfberry ([digestive enzyme]: [wolfberry]) is preferably 1: 0.001 or more and 1: 100 or less, more preferably 1: 0. 0.003 or more and 1:10 or less, and particularly preferably 1: 0.01 or more and 1: 1 or less.

  Particularly when the composition contains chia seeds, the dry mass ratio of digestive enzyme and chia seeds ([digestive enzyme]: [chia seeds]) is preferably 1: 0.001 or more and 1: 100 or less, more preferably 1: 0. 0.003 or more and 1:10 or less, and particularly preferably 1: 0.01 or more and 1: 1 or less.

  Especially when the composition contains baobab, the dry mass ratio of digestive enzyme and baobab ([digestive enzyme]: [baobab]) is preferably 1: 0.001 or more and 1: 100 or less, more preferably 1: 0. 0.003 or more and 1:10 or less, and particularly preferably 1: 0.01 or more and 1: 1 or less.

  In particular, when the composition contains hemp, the dry mass ratio of digestive enzyme and hemp ([digestive enzyme]: [hemp]) is preferably 1: 0.001 or more and 1: 100 or less, more preferably 1: 0. 0.003 or more and 1:10 or less, and particularly preferably 1: 0.01 or more and 1: 1 or less.

  In particular, when the composition contains cam cam, the dry mass ratio of digestive enzyme and cam cam ([digestive enzyme]: [cam cam]) is preferably 1: 0.001 or more and 1: 100 or less, more preferably 1: 0. 0.003 or more and 1:10 or less, and particularly preferably 1: 0.01 or more and 1: 1 or less.

  In particular, when the composition contains maquiberry, the dry mass ratio of digestive enzyme and maquiberry ([digestive enzyme]: [maquiberry]) is preferably 1: 0.001 or more and 1: 100 or less, more preferably 1: 0. 0.003 or more and 1:10 or less, and particularly preferably 1: 0.01 or more and 1: 1 or less.

  Particularly when the composition contains golden berry, the dry mass ratio of digestive enzyme and golden berry ([digestive enzyme]: [golden berry]) is preferably 1: 0.001 or more and 1: 100 or less, more preferably It is 1: 0.003 or more and 1:10 or less, and particularly preferably 1: 0.01 or more and 1: 1 or less.

  Particularly when the composition contains mulberry, the dry mass ratio of digestive enzyme and mulberry ([digestive enzyme]: [mulberry]) is preferably 1: 0.001 or more and 1: 100 or less, more preferably 1: 0. 0.003 or more and 1:10 or less, and particularly preferably 1: 0.01 or more and 1: 1 or less.

  Particularly when the composition contains pomegranate, the dry mass ratio of digestive enzyme and pomegranate ([digestive enzyme]: [pomegranate]) is preferably 1: 0.001 or more and 1: 100 or less, more preferably 1: 0. 0.003 or more and 1:10 or less, and particularly preferably 1: 0.01 or more and 1: 1 or less.

  Particularly when the composition contains dragon fruit, the dry mass ratio of digestive enzyme and dragon fruit ([digestive enzyme]: [dragon fruit]) is preferably 1: 0.001 or more and 1: 100 or less, more preferably It is 1: 0.003 or more and 1:10 or less, and particularly preferably 1: 0.01 or more and 1: 1 or less.

  Particularly when the composition contains aronia, the dry mass ratio of digestive enzyme and aronia ([digestive enzyme]: [aronia]) is preferably 1: 0.001 or more and 1: 100 or less, more preferably 1: 0. 0.003 or more and 1:10 or less, and particularly preferably 1: 0.01 or more and 1: 1 or less.

  In particular, when the composition contains noni, the dry mass ratio of digestive enzyme and noni ([digestive enzyme]: [noni]) is preferably 1: 0.001 or more and 1: 100 or less, more preferably 1: 0. 0.003 or more and 1:10 or less, and particularly preferably 1: 0.01 or more and 1: 1 or less.

  Particularly when the composition contains chlorella, the dry mass ratio of the digestive enzyme and chlorella ([digestive enzyme]: [chlorella]) is preferably 1: 0.001 or more and 1: 100 or less, more preferably 1: 0. 0.003 or more and 1:10 or less, and particularly preferably 1: 0.01 or more and 1: 1 or less.

  In particular, when the composition contains kale, the dry mass ratio of digestive enzyme and kale ([digestive enzyme]: [kale]) is preferably 1: 0.001 or more and 1: 100 or less, more preferably 1: 0. 0.003 or more and 1:10 or less, and particularly preferably 1: 0.01 or more and 1: 1 or less.

  Particularly when the composition contains broccoli sprout, the dry mass ratio of digestive enzyme and broccoli sprout ([digestive enzyme]: [broccoli sprout]) is preferably 1: 0.001 or more and 1: 100 or less, more preferably It is 1: 0.003 or more and 1:10 or less, and particularly preferably 1: 0.01 or more and 1: 1 or less.

  In particular, when the composition contains aloe vera, the dry mass ratio of digestive enzyme and aloe vera ([digestive enzyme]: [aloe vera]) is preferably 1: 0.001 or more and 1: 100 or less, more preferably 1: 0. 0.003 or more and 1:10 or less, and particularly preferably 1: 0.01 or more and 1: 1 or less.

  In particular, when the composition contains wheatgrass, the dry mass ratio of digestive enzyme and wheatgrass ([digestive enzyme]: [wheatgrass]) is preferably 1: 0.001 to 1: 100, more preferably It is 1: 0.003 or more and 1:10 or less, and particularly preferably 1: 0.01 or more and 1: 1 or less.

  The total amount of digestive enzymes and superfoods in the composition is preferably 0.001 to 99 mass%, more preferably 0.01 to 50 mass%, more preferably 0.1 mass% in the composition. % To 30% by mass is particularly preferable. The preferred total amount of papain and superfood content and the preferred total amount of α-amylase and superfood content in the composition are the same as the preferred total amount of the digestive enzyme and superfood content.

  The total amount of digestive enzyme and superfood content is the dry mass of digestive enzyme and superfood, with the lower limit as the amount used per day, for example, 0.03 mg or more, preferably 0.3 mg or more, more preferably 3 mg. It can set so that it may become above. In addition, the upper limit of the amount used per day can be set to be, for example, 10000 mg or less, preferably 5000 mg or less, more preferably 3000 mg or less, based on the dry mass of the digestive enzyme and superfood.

  Specifically, the content of the active ingredient is 0.3 mg or more and 5000 mg or less, preferably 3 mg or more and 3000 mg or less in terms of the dry mass of the digestive enzyme and superfood as the amount used per day. However, when the composition and agent of the present invention contain protease, amylase activity, lipase activity, cellulase activity, galactosidase activity or other substances having their promoting action, the inclusion of digestive enzymes and superfoods accordingly It can be adjusted as appropriate, such as reducing the amount.

  By including the digestive enzyme and the superfood, the composition of the present invention has excellent digestive enzyme activities such as protease activity and amylase activity, as demonstrated by Examples described later.

  The form of the oral composition of the present invention is, for example, a form suitable for oral use, specifically, granular, powder, tablet, chewable, capsule, soft capsule, liquid, syrup And the like.

  The packaging form of the composition and agent of the present invention is not particularly limited, and can be appropriately selected depending on the dosage form. For example, blister packs such as PTP; strip packaging; heat seal; aluminum pouch; Examples include film packaging used; glass containers such as vials; plastic containers such as ampoules.

  The degree of protease activity in the composition of the present invention is not particularly limited. For example, the proteolytic titer obtained by the method described in the examples described later is preferably 50 units / g or more, more preferably 100 units / g. More preferably, it is 1,000 units / g or more, still more preferably 10,000 units / g or more, and particularly preferably 100,000 units / g or more.

In addition, for example, amylase and processed food containing the same preferably have a starch degradation titer described later of 1 to 5000000 units / g, preferably 10 to 500000 units / g, and 100 to 100000 units / g. It is more preferable that
The starch degradation titer can be measured by the method described in Examples described later.

In addition, the composition of the present invention contains a digestive enzyme and a superfood, thereby providing a nutrient absorption promoter and a proteolytic agent through enzyme actions such as high protease activity, amylase activity, cellulase activity, galactosidase activity, and lipase activity. , Protein activity regulators, starch degrading agents, and the like may be employed.
In general, it is said that when the body becomes deficient in enzymes, metabolism in the body decreases because metabolic enzymes are used preferentially for digestion of food. It is said that when digestive enzymes are supplied into the body by ingestion, the production of metabolic enzymes in the body is increased or the consumption thereof is suppressed, thereby increasing or improving metabolism, thereby providing a diet effect. In addition, when digestive enzymes are supplied into the body by ingestion, the digestive power in the digestive organs is improved, which leads to intestinal regulation, and constipation and abdominal obesity are easily resolved.
Since the composition of the present invention has high digestive enzyme activity, it is expected to increase these diet effects and the effects of eliminating constipation or abdominal obesity by supplying them into the body by oral ingestion.

  The composition of the present invention is suitably applied to humans, but is not particularly limited as long as the expected effects are exhibited, and can be applied to animals other than humans. The user of the composition of the present invention is not particularly limited, and may be, for example, a healthy person, but may be a person who is expected to have physiological activity by digestive enzymes such as protease, amylase, cellulase, galactosidase and lipase. Preferably, middle-aged people over 40 years old are more preferable. The frequency of use of the composition of the present invention is not particularly limited, and is, for example, once or more per week, and preferably twice or more per week.

  In addition to the active ingredient, the composition of the present invention can contain a digestive enzyme such as protease, amylase, cellulase, galactosidase, and lipase, or a second physiologically active ingredient that exhibits their promoting action. Such a second physiologically active ingredient is not particularly limited as long as it exhibits a known activity of promoting protease activity. For example, the active ingredient of the composition and agent which show protease activity promotion effect of patent document 1 is mentioned. By containing the second physiologically active ingredient in addition to the specific ingredient of the present invention, the composition of the present invention can be a composition showing a synergistic protease activity promoting action. The second physiologically active component can be one or more components. The blending amount of the second physiologically active ingredient is not particularly limited as long as it does not hinder the solution of the problems of the present invention, and is appropriately adjusted.

  The production method of the composition and agent of the present invention is not particularly limited, and is produced according to a general production method known to those skilled in the art depending on the use mode. For example, for granules and solids, fluidized bed granulation method, stirring granulation method, or those mixed with the other components or the second physiologically active component at the same time or divided into several stages, It can be granulated by granulation according to a granulation method such as extrusion granulation, and further formed into a tablet by compression molding according to a conventional method using a tableting machine or the like.

  Hereinafter, the present invention will be described in more detail with reference to examples. However, the present invention is not limited to these examples, and the present invention can take various modes as long as the problems of the present invention can be solved. .

[Examples 1 to 20, Comparative Examples 1 to 22: measurement of protease activity]
It was demonstrated as follows that a composition containing papain, which is a digestive enzyme, and also containing superfoods has exceptionally remarkable protease activity.

(1) Test sample The following were used as test samples.
(1-1) Papain: A commercially available papaya extract powder (proteolytic titer: 90,000 units / g) extracted from immature papaya seeds was used.
(1-2) The following superfood powder was used as a superfood.
Coconut: Commercially available coconut milk powder (squeezed coconut endosperm and dried by spray drying)
・ Acai: Commercial acai powder (dried powder of acai fruit)
・ Cacao: Commercial cocoa extract powder (pulverized hydrated ethanol extract of cocoa seeds)
Spirulina: Commercially available Spirulina powder (algae dried and pulverized powder) was extracted with 60% by volume ethanol aqueous solution at room temperature (25 ° C, the same applies hereinafter) for 16 hours, and the resulting extract was dried under reduced pressure.・ Moringa: A commercially available moringa leaf powder (which is obtained by drying and pulverizing the leaf) was extracted with a 60 vol% aqueous ethanol solution at room temperature for 16 hours, and the resulting extract was dried under reduced pressure. Powdered maca: The dried and ground powder of commercially available maca root was extracted with water at room temperature for 16 hours, and the resulting extract was pulverized by vacuum drying treatment. Amaranthus (1): Commercially available Amaranthus powder (amaranthus seeds dried and pulverized) was extracted with 60 vol% ethanol aqueous solution at room temperature for 16 hours, and the resulting extract was pulverized by drying under reduced pressure. Nussus (2): Commercially available amaranth powder (amaranth seeds dried and pulverized) is extracted with water at room temperature for 16 hours, and the resulting extract is pulverized by drying under reduced pressure. : Commercial wolfberry juice powder (obtained by drying powdered wolfberry juice) at room temperature for 16 hours, and the resulting extract was powdered by vacuum drying treatment. Powder (chia seed dry pulverized powder) was extracted with water at room temperature for 16 hours, and the resulting extract was pulverized by drying under reduced pressure. Baobab: Commercial baobab fruit powder (dried powder of baobab fruit) Was extracted with 60% by volume ethanol aqueous solution at room temperature for 16 hours, and the resulting extract was pulverized by drying under reduced pressure. Makiberry: Dry ground powder of commercially available fruit Chlorella: Commercially available chlore Powdered powder (dried and ground powder of algal cells) was extracted with 60 vol% ethanol aqueous solution at room temperature for 16 hours, and the resulting extract was pulverized by drying under reduced pressure. Wheatgrass: Drying of young wheat shoots The pulverized powder was extracted with 60 volume% ethanol aqueous solution at room temperature for 16 hours, and the resulting extract was pulverized by drying under reduced pressure. ・ Dragon Fruit: Fruit juice with seeds and skins removed from the market Dry powder of noni: A commercially available noni leaf dried pulverized powder was extracted with 60 vol% ethanol aqueous solution at room temperature for 16 hours, and the resulting extract was pulverized by drying under reduced pressure. Kale: commercially available Kale leaf dry ground powder was extracted with 60 vol% ethanol aqueous solution at room temperature for 16 hours, and the resulting extract was pulverized by vacuum drying treatment. Juice squeezed from Loebella mesophyll by pulverization under reduced pressure ・ Aronia: Commercially available Aronia ・ Meranocarpa fruit dried and ground powder ・ (1-3) Garlic powder: Commercial garlic (garlic bulb) Extracted for 16 hours at room temperature, and the resulting extract was pulverized by drying under reduced pressure

(2) Protease activity measurement (2-1) Preparation of tyrosine calibration curve After tyrosine was dried at 105 ° C. for 3 hours, 0.100 g was accurately weighed and 0.2N hydrochloric acid was added to dissolve it. This was used as a tyrosine standard solution. 100 μl of this tyrosine standard solution was accurately weighed, diluted to 5 ml with 0.2N hydrochloric acid (20 μg / ml), diluted to 15, 10 and 5 μg / ml to obtain a calibration curve sample solution.

  To 200 μl of each solution of 0.2N hydrochloric acid and a calibration curve reagent solution, 500 μl of 0.55M sodium carbonate and 100 μl of Fine reagent were added and incubated at 37 ° C. for 30 minutes. Here, the Foline reagent was prepared by dissolving 5 g of sodium tungstate and 1 g of phosphomolybthenic acid in 50 ml of pure water, adding 2.5 ml of phosphoric acid, refluxing extraction, and making up to 200 mL. The test was carried out with 2 repetitions.

200 μl each was transferred to a 96-well plate, and the respective absorbances A ₀ , A ₁ , A ₂ , A ₃ and A ₄ at 660 nm were measured. Here, the tyrosine concentrations of A ₀ , A ₁ , A ₂ , A ₃ and A ₄ are ₀ , ₅ , ₁₀ , 15 and 20 μg / ml, respectively.

Regarding the measured values, a calibration curve was prepared by taking the absorbance difference (A _n −A ₀ ) on the vertical axis and the tyrosine concentration (μg / ml) of each solution on the horizontal axis. The amount of tyrosine ([F] μg / ml) with respect to the absorbance difference of 1.000 was calculated from the obtained calibration curve.

(2-2) Measurement of proteolytic titer A casein solution was prepared as follows. That is, about 1 g of casein (derived from bovine milk (Hammarsten formulation); Wako Pure Chemical Industries, Ltd.) was accurately weighed and incubated at 105 ° C. for 2 hours, and then the dry weight was measured. The obtained dry casein equivalent to 1.20 g was accurately weighed, 160 ml of 0.05 M aqueous solution of disodium monohydrogen phosphate was added, and the mixture was dissolved in a water bath by heating at 40 ° C. for about 15 minutes. The casein solution was prepared by adjusting to pH 7.5 using 1M hydrochloric acid and making up to 200 ml with ultrapure water.

  A solution containing 0.11 M trichloroacetic acid, 0.22 M sodium acetate and 0.33 M acetic acid was prepared as a protein precipitation solution using ultrapure water as a solvent.

  A solution containing 0.01 M sodium chloride, 0.002 M calcium acetate and 0.002 M calcium sulfate was prepared as an enzyme-diluted solution using ultrapure water as a solvent.

  A test sample solution was prepared by dissolving the test sample shown in Table 1 or 2 below in 1 ml of the enzyme dilution solution so as to have the concentration shown in the same table.

  500 μl of casein solution was incubated at 37 ° C. for 10 minutes, 100 μl of the test sample solution was added, and immediately shaken and mixed. The resulting solution was incubated at 37 ° C. for 10 minutes (pH in solution at the time of incubation = 6.0). After adding 500 μl of protein precipitation solution and incubating at 37 ° C. for 30 minutes, it was centrifuged at 10,000 rpm for 3 minutes at room temperature.

  Also, as a blank, add 500 μl of protein precipitation solution to 100 μl of test sample solution, mix and then add 500 μl of casein solution, incubate at 37 ° C. for 30 minutes, and then incubate at 10,000 rpm for 3 minutes at room temperature. Centrifuged.

To 200 μl of the centrifugal supernatant, 500 μl of 0.55M sodium carbonate and 100 μl of Foline reagent were added and incubated at 37 ° C. for 30 minutes. 200 μL of the solution after incubation was transferred to a 96-well plate, and the absorbance (A _T ) at 660 nm was measured.
Moreover, the absorbance at a blank was A _B.

Based on the resulting absorbance, the proteolytic titer was calculated using the following formula:
Proteolytic titer (unit / g) = (A _T −A _B ) × F × (amount of reaction solution) × (1/10) × (1 / W)
F: Tyrosine amount (μg / ml) when the absorbance difference obtained from the tyrosine calibration curve is 1.000
Reaction solution: casein solution + test sample solution + protein precipitation solution W: amount of sample in reaction solution (g)

  From the measured proteolytic titer, it was confirmed that the papaya extract of Comparative Example 1 was an active protease. Further, the proteolytic titer of other test sample solutions was measured by the above method. Tables 1 and 2 show the relative values of the proteolytic titers of the compositions of Examples and Comparative Examples when the proteolytic titer of Comparative Example 1 is 100%, as relative titers to papain. In Tables 1 and 2, ◯ indicates that the test sample solution contains the test substance described in the table in the amount described in the table.

  As shown in the said Table 1 and Table 2, the composition of each Example improved protease activity significantly compared with the comparative example 1 which contains a papain independently by containing a superfood with a papain. On the other hand, in the case of the superfoods of Comparative Examples 3 to 22 alone, almost no protease activity was obtained, and the composition of Comparative Example 2 containing garlic and papain also had protease activity compared to Comparative Example 1. There was no improvement. As mentioned above, it turns out that the digestive enzyme activity improvement effect by the composition for oral use of this invention is excellent.

[Examples 21 to 39, Comparative Examples 23 to 42: Measurement of amylase activity]
It was demonstrated as follows that the composition containing superfood in addition to α-amylase, which is a digestive enzyme, has a particularly remarkable amylase activity compared to α-amylase alone.

(1) Test sample The following were used as test samples.
(1-1) α-amylase, a commercially available enzyme preparation powder (starch degradation titer: ˜30 units / mg) was used. Here, 1 unit corresponds to the amount of enzyme that liberates 1 μmol of maltose at a pH of 6.0 at 25 ° C. for 1 minute using starch as a substrate.

(1-2) The following superfood powder was used as a superfood.
-Baobab: A commercially available baobab fruit powder (dried and ground powder of baobab fruit) was extracted with 60 vol% ethanol aqueous solution at room temperature for 16 hours, and the resulting extract was pulverized by a drying process under reduced pressure.-Amaranthus: Commercially available Amaranthus seeds were dried and pulverized with 60 vol% ethanol aqueous solution at room temperature for 16 hours, and the resulting extract was pulverized by drying under reduced pressure. Spirulina: Commercially available Spirulina powder (dried algal bodies) (Pulverized powder) was extracted with 60 vol% ethanol aqueous solution at room temperature for 16 hours, and the resulting extract was pulverized by drying under reduced pressure. Coconut: Commercial coconut milk powder (coconut endosperm Squeezed and made into dry powder by spray-drying process)
・ Cacao: Commercial cocoa extract powder (pulverized hydrated ethanol extract of cocoa seeds)
Moringa: Commercially available Moringa leaf powder (dried and pulverized leaves) was extracted with 60 vol% ethanol aqueous solution at room temperature for 16 hours, and the resulting extract was pulverized by drying under reduced pressure. Wolfberry: A commercially available wolfberry fruit juice powder (obtained by drying powder of wolfberry fruit juice) is extracted with 60 vol% ethanol aqueous solution at room temperature for 16 hours, and the resulting extract is pulverized by drying under reduced pressure. Takomono / Acai: Acai powder on the market (dry powder of acai fruit)
・ Maka: A dried and ground powder of commercially available maca root was extracted with water at room temperature for 16 hours, and the resulting extract was pulverized by drying under reduced pressure. ・ Dragon fruit: Seed and skin of commercially available dragon fruit The dried fruit juice juice powder was removed. Noni: The dried and ground powder of commercially available noni leaves was extracted with 60 vol% ethanol aqueous solution at room temperature for 16 hours, and the resulting extract was pulverized by drying under reduced pressure. Mono-Kale: Dry kneaded powder of commercially available Kale leaf was extracted with 60 vol% ethanol aqueous solution at room temperature for 16 hours, and the resulting extract was pulverized by drying under reduced pressure. Aloe Vera: Commercial Aloe Vera Juice extracted from mesophyll powdered by drying under reduced pressure ・ Aronia: Commercial aronia ・ Dry pulverized fruit of melanocalpa ・ Camcam: Drying of commercial camcam fruit With the end of grinding.
-Golden berry: The dry ground powder of the fruit of the commercially available golden berry was used.
-Mulberry: A dry pulverized powder of commercially available mulberry fruit was used.
-Pomegranate: A dry pulverized powder of commercially available pomegranate fruit was used.
-Broccoli sprout: A dry pulverized powder of commercially available broccoli sprout was used.
(2) <Measurement of α-amylase activity>
(2-1) Sample preparation Acetic acid and sodium acetate were dissolved in water to prepare a 20 mM acetate buffer having a pH of 5 at room temperature. Starch (Wako Pure Chemicals: 191-03985) was dissolved in 0.5 mg / ml in the prepared 20 mM acetate buffer to give a starch solution.
Potassium iodide was dissolved in 1 M hydrochloric acid to 1 mg / ml, and then iodine was dissolved to 0.1 mg / ml to obtain an iodine solution.
A test sample solution was prepared by dispersing or dissolving the test sample shown in Table 3 or 4 below in the 20 mM acetate buffer so as to have a concentration shown in the same table.

(2-2) Calibration curve Using 20 mM acetate buffer and 0.5 mg / ml starch solution, the starch concentrations were 0 mg / ml, 0.06 mg / ml, 0.125 mg / ml, 0.25 mg / ml and 0. A starch solution adjusted to each concentration of 5 mg / ml was prepared. To 1 ml of starch solution of each concentration, 125 μl of 20 mM acetate buffer and 125 μl of iodine solution were added in order, and the absorbance at 620 nm was measured to prepare a calibration curve.

(2-3) Measurement of starch degradation titer 1 ml of 0.5 mg / ml starch solution is kept at 30 ° C., and 125 μl of the test substance solution prepared in (2-1) is added to start the reaction. 125 μl was added and mixed well to obtain a solution with an enzyme reaction of 20 minutes.
Separately, 125 ml of iodine solution and 125 μl of the test substance solution were added in order to 1 ml of 0.5 mg / ml starch solution and mixed well to obtain a solution with 0 minute enzyme reaction. The absorbance at 620 nm of each of the enzyme reaction 0 minute solution and the enzyme reaction 20 minute solution was measured.
Based on the absorbance difference Δ between the enzyme reaction 0 minutes and 20 minutes, the starch equivalent value digested by the test sample was calculated based on the calibration curve obtained in (2-2).
About the obtained starch equivalent value, the relative value when (alpha) -amylase single-piece | unit (comparative example 1) was made into 100% was calculated | required, and it was set as the starch degradation titer (relative value). The results are shown in Table 3 below. In the table below, ○ indicates that the test sample solution prepared in the above (2-1) contained the test sample described in the left column at the concentration described in the same table (in Table 4 below). The same).

  As shown in Tables 3 and 4 above, the composition of each example contained a superfood together with α-amylase, so that the protease activity was significantly higher than that of Comparative Example 23 containing α-amylase alone. Improved. In contrast, the compositions of Comparative Examples 24-42 containing each superfood alone did not show amylase activity. As mentioned above, it turns out that the digestive enzyme activity improvement effect by the composition for oral use of this invention is excellent.

<Production Examples 1-10>
Granules containing papain and superfood were produced with the formulation shown in Table 5 below.

<Production Examples 11 to 21>
Granules containing papain and superfood were produced with the formulation shown in Table 6 below.

<Production Examples 22 to 29>
A powdered beverage containing papain and superfoods was prepared with the formulation shown in Table 7 below.

<Production Examples 30 to 37>
In the formulation shown in Table 8 below, papain and superfood were blended and encapsulated with a film containing gelatin and glycerin to produce a soft capsule.

<Production Examples 38 to 45>
In the formulation shown in Table 9 below, papain and superfood were blended and encapsulated with a film containing gelatin to produce a hard capsule.

<Production Examples 46-53>
Granules containing papain and superfood were produced with the formulation shown in Table 10 below.

<Production Examples 54 to 61>
Tablets containing papain and superfood were produced with the formulation shown in Table 11 below.

<Production Examples 62 to 72>
A powdered beverage containing papain and superfoods was prepared according to the formulation shown in Table 12 below.

<Production Examples 73 to 83>
Granules containing papain and superfood were produced with the formulation shown in Table 13 below.

<Production Examples 84 to 97>
A powdered beverage containing α-amylase and superfood was produced with the formulation shown in Table 14 below.

<Production Examples 98 to 111>
Granules containing α-amylase and superfood were produced with the formulation shown in Table 15 below.

References:
(1) Activity measurement method by notification of Central Pharmaceutical Affairs Council No. 523; Examination of protein digestion measurement method for protease and papain preparations for food production (2) Edited by Japan Standards Association: revised cold medicine / antipyretic analgesic test method ( Appendix 2)-Gastrointestinal antacid / pH test method and digestion test method and explanation-

ADVANTAGE OF THE INVENTION According to this invention, the oral composition with high digestive enzyme activities, such as protease, is obtained, and it is useful for those who expect the physiological activity by a digestive enzyme, the food / beverage products for specific health, the nutrition function food / beverage products, health Functional foods, special-purpose foods, nutritional supplements, health supplements, supplements, beauty foods, other health foods, quasi drugs, cosmetics, and pharmaceuticals.

Claims (1)

An oral composition containing a digestive enzyme and a superfood,
The digestive enzyme is papain,
Super hood, spirulina, chlorella, amaranth, oral compositions is at least one extract selected or found wheatgrass and cable Le.