JP6476799B2 - Method of manufacturing needle-like body - Google Patents

Description

  The present invention relates to a method of manufacturing needles. In particular, the present invention relates to a method for producing a needle-like body applied to the skin.

  The percutaneous absorption method, which is a method of permeating a delivery substance such as a drug from above the skin and administering the delivery substance into the body, is known as a method by which the delivery substance can be conveniently administered to the human body without causing pain. .

  In the field of transdermal administration, there has been proposed a method of perforating the skin using a needle-like body formed in the order of μm and administering a drug or the like into the skin (see Patent Document 1).

  As a method for producing a needle-like body, it has been proposed that an original plate be prepared using mechanical processing, a transfer plate be formed from the original plate, and transfer processing and molding be performed using the transfer plate (see Patent Document 2). ).

  Further, as a method of producing a needle-like body, it has also been proposed to create an original plate using an etching method, to form a transfer plate from the original plate, and to perform transfer processing and molding using the transfer plate (Patent Document 3) reference).

  It is desirable that the material constituting the needle-like body is a material that does not adversely affect the human body even if the broken needle-like body remains in the body. Such a material is biocompatible with chitin, chitosan, etc. Materials have been proposed (see Patent Document 4).

  When producing a needle-like body using such a biocompatible material, it is generally prepared by filling a solution of the material into a transfer plate and removing the solvent by a method such as drying.

  As a material of the transfer plate, a water repellant resin such as silicone resin having good releasability is often used. (See Patent Document 5)

Japanese Patent Application Laid-Open No. 48-93192 WO 2008/013282 pamphlet WO 2008/004597 pamphlet WO 2008/020632 pamphlet JP, 2013-153866, A

  However, when it is attempted to produce a needle-like body using a transfer plate (intaglio) formed of a water repellent resin, the aqueous solution forming the needle-like body filled in the transfer plate shrinks upon drying due to the water repellency of the transfer plate. It may be difficult to make needles in the entire transfer area. In particular, when a transfer plate made of a water repellent resin having a large area of the transfer region is used to produce a needle-like body having a large area in the planar direction, the shrinkage tendency of the aqueous solution becomes remarkable.

  Therefore, an object of the present invention is that even when a transfer plate (intaglio) made of a water repellent resin having a large transfer area area is used, there is almost no thickness unevenness without shrinkage of the aqueous solution for forming a needle-like body during drying. It is an object of the present invention to provide a method of manufacturing needles.

  The present invention is a method for producing a needle-like body comprising a base and a projection on one surface of the base, wherein a step of preparing a needle-like body forming aqueous solution containing a needle-like body forming material, and the needle Preparing an intaglio plate formed of a material containing a water repellent resin and having a concave portion corresponding to the shape of the protrusion of the green body, supplying the aqueous solution for forming a needle-like body to the intaglio plate, and supplying the intaglio plate The method further includes the steps of drying the aqueous solution for forming the needle-like body and peeling the needle-like material formed by the drying from the intaglio plate, and the concave side of the intaglio plate so that the aqueous solution for forming needle-like body does not shrink in the drying process. The present invention relates to a method for producing a needle-like body, wherein a linear groove is provided on the surface of the needle.

  In the method of manufacturing the needle-like body, the water repellent resin is preferably a silicone resin or a fluorine resin.

  According to the present invention, it is possible to manufacture a needle-like body having a large area in the planar direction and having almost no unevenness in thickness. As a result, the productivity of high quality needles can be improved, and the production cost can be reduced.

It is a figure for demonstrating the 2nd process to a 5th process among five processes in the manufacturing method of this invention. It is a figure for demonstrating the method to produce an intaglio from an original plate. It is a figure for demonstrating the structure of an original edition. It is a figure which shows an example of the intaglio used for the manufacturing method of this invention. It is a perspective view which shows an example of the intaglio plate used for the manufacturing method of this invention. It is a perspective view of the needle-like body produced using the intaglio plate shown in FIG.

  Hereinafter, embodiments of the present invention will be described in detail. Although the drawings will be referred to as appropriate in the following description, the embodiments described in the drawings are illustrative of the present invention, and the present invention is not limited to the embodiments described in these drawings.

  Moreover, in this specification, when expressing a numerical range using "-", the numerical value of the upper limit shown by "-" and a lower limit shall also be included in a numerical range.

  The method for producing the needle-like body of the present invention comprises a step (first step) of preparing a needle-like body forming aqueous solution containing a needle-like body-forming material, and a recess corresponding to the shape of the protrusion of the needle-like body. A step (second step) of preparing an intaglio formed from a material containing a water repellent resin, a step (third step) of supplying the aqueous solution for forming a needle-like body to the intaglio, and acicular body formation supplied to the intaglio Drying the aqueous solution (the fourth step), and removing the needle-like body formed by the drying from the intaglio plate (the fifth step), so that the aqueous solution for forming the needle-like body does not shrink in the fourth step A linear groove is provided on the surface on the concave side of the intaglio. In the manufacturing method of the present invention, the order of the first step and the second step is not limited and can be interchanged.

  The needle-like body manufactured by the method of the present invention can be used in various ways, for example, as a MEMS device, an optical member, a sample jig, a medicine, or as a part used in medical, cosmetic and cosmetic applications. Below, the case where a needlelike body is applied to skin is demonstrated as an example.

<Step of Preparing Needle-Forming Aqueous Solution (First Step)>
First, the needle-forming material is dissolved in water to prepare a needle-forming aqueous solution.

  The needle-forming material is preferably a biodegradable polysaccharide, such as dextran, dextrin, pectin, pullulan, chondroitin sulfate, alginate, chitosan, carboxymethylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, methylcellulose, ethylcellulose and so on. In addition, a mixture of these biodegradable polysaccharides may be used. By using biodegradable polysaccharides, it is possible to dissolve the projections in the skin after piercing the needle with the skin.

  Further, when preparing the aqueous solution for forming a needle-like body, a desired substance to be delivered into the skin (hereinafter, also referred to as a "delivery substance") may be dissolved in water together with the above-mentioned needle-like body forming material.

  Moreover, when adjusting a needle-like body formation liquid, you may also contain solvents other than water as a solvent. As solvents other than water, alcohols such as ethanol can be exemplified.

  As a delivery substance, for example, a pharmacologically active substance or a cosmetic substance can be used. At this time, when a material having a fragrance is used as a delivery substance, an odor can be given at the time of use, and a needle-like body can be used as a cosmetic.

  The pharmacologically active substance may be further selected appropriately depending on the use. For example, it may be a vaccine such as influenza, a pain medicine for cancer patients, insulin, a biologic, a gene therapy, an injection, an oral preparation, a skin preparation and the like. Since the needles produced by the method of the present invention puncture the skin, pharmacologically active substances that require subcutaneous injection can be applied in addition to the pharmacologically active substances used for conventional transdermal administration. In the case of using a needle-like body, a vaccine applied to children and the like may be applied as a pharmacologically active substance because pain from puncture is reduced as compared to injection. In addition, it is difficult for children to take oral medications by conventional oral administration, but when using needles, there is no need to take medications during administration, and thus such oral medications are pharmacologically active. It may be applied as a substance.

  The cosmetic substance is a substance used as a cosmetic and a cosmetic product, and is, for example, physiologically active exhibiting a moisturizing agent, a coloring agent, a fragrance, a cosmetic effect (ameliorating effect on wrinkles, acne, gestational line etc., ameliorating effect on hair loss etc) Substances etc. may be mentioned.

  Further, when preparing the aqueous solution for forming the needle-like body, two kinds of an aqueous solution forming the protrusion of the needle-like body and an aqueous solution forming the base of the needle-like body may be prepared. Since the base of the needle-like body hardly elutes inside the skin, when the delivery substance is expensive, the aqueous solution forming the protrusion contains the delivery substance, and the aqueous solution forming the base has the delivery substance You may prepare the needle-like body formation aqueous solution which is not made to contain, respectively. Thereby, the manufacturing cost of the needle-like body can be reduced.

  Next, the second to fifth steps in the manufacturing method of the present invention will be described.

  FIG. 1 is a figure for demonstrating the 2nd process to a 5th process among five processes in the manufacturing method of this invention. That is, in FIG. 1, (a) is provided with a recess 12 corresponding to the shape of the protrusion of the needle and a linear groove 14 so that the aqueous solution for forming the needle does not shrink in the fourth step. The step (second step) of preparing the intaglio 10 formed of the material containing the resin, the step (b) is a step (third step) of supplying the needle material forming aqueous solution 16 prepared in the first step to the intaglio 10 c) drying the acicular material forming aqueous solution 16 supplied to the intaglio 10 in the third step (fourth step); (d) peeling the acicular member 20 formed by drying in the fourth step from the intaglio It is a figure explaining the process (5th process) to do.

  Each step will be described below with reference to FIG. 1 as appropriate.

<Step of preparing intaglio (second step)>
In this step, as shown in FIG. 1A, a recess 12 corresponding to the shape of the protrusion of the needle-like body and a linear groove 14 such that the aqueous solution for forming the needle-like body does not shrink in the fourth step An intaglio 10 formed of a material containing a water repellent resin is prepared.

  A known method can be used as a method of forming the intaglio 10.

  FIG. 2 is a view for explaining a method of using the original plate 32 as a method of forming the intaglio 10.

  First, the substrate 30 is prepared (FIG. 2 (a)), and processed to conform to the shape, size, and arrangement of the projections of the needle-like body from which it is manufactured, as shown in FIG. 2 (b) , The original plate 32 provided with the projection part 34 is produced.

  The material of the substrate 30 is not particularly limited, and it is desirable to select the material in view of processing suitability, availability of the material, and the like. Specifically, crystal materials such as silicon, silicon carbide and quartz; ceramics such as alumina, aluminum nitride and machinable ceramics; organic materials such as acrylic and polyacetal; metallic materials such as nickel and aluminum; glass etc. .

  As a processing method, a lithography method, a wet etching method, a dry etching method, a sand blast method, a laser processing method, a precision machining method, or the like can be used.

  Below, the original plate 32 to produce is demonstrated.

  FIG. 3 is a view for explaining the configuration of the original plate, (a) is a schematic view showing a part of the original plate 32, (b) is a plan view of (a), (c) ) Is a cross-sectional view taken along the line II 'in (b).

  As shown in FIG. 3A, the original plate 32 includes a base 38 and a protrusion 34 on one surface of the base 38.

  The base 38 has a role of a base for supporting the projection 34, and the surface is usually flat and shaped like a plate.

  Protrusions 34 conform in shape, size, and placement on base 38 to those of the needles produced by the method of the present invention.

  The protrusion 34 may have any shape that is suitable for piercing the skin, and may be designed as appropriate. The shape of the projection 34 may be, for example, a cone, a pyramid, a cylinder, a prism, a pencil (the body is columnar and the tip is pyramidal). Also, the number of protrusions 34 on one surface of the base 38 may be one or more.

  In the case where a plurality of protrusions 34 are provided on one surface of the base 38, the protrusions 34 are preferably arranged in an array. Here, “array-like” means that the protrusions 34 are aligned, and includes, for example, a lattice arrangement, a close-packed arrangement, a concentric arrangement, a random arrangement, and the like. FIG. 3 shows the case where the protrusions 34 are arranged in a lattice.

  When the protrusions 34 are regularly arranged, the distance W between the protrusions 34 and the protrusions 34 is preferably 50 μm to 2000 μm. Here, the distance W between the protrusion 34 and the protrusion 34 means the closest distance between the protrusion 34 and the protrusion 34 as shown in FIG. 3C.

  Preferably, the needles produced according to the invention have a profile and a length suitable for forming the puncture in the skin of the projections. Therefore, in the original plate 32 corresponding to the needle-like body, the height H of the protrusion 34 is preferably 10 μm to 700 μm. Here, the height H of the protrusion 34 is a distance from the top surface of the base 38 to the tip of the protrusion 34 as shown in FIG.

  Further, the height H of the protrusion 34 may be determined in consideration of how deep the puncture hole formed when puncturing the needle-like body manufactured by the method of the present invention is to be formed in the skin. it can.

  In particular, when the puncture hole formed when puncturing the needle-like body is retained in the “cornerous layer”, the height H of the protrusion 34 is preferably 10 μm to 300 μm, more preferably 30 μm to 200 μm.

  In addition, when the puncture hole formed when the needle-like body is punctured is kept “to penetrate the stratum corneum and to a length that does not reach the nerve layer”, the height H of the protrusion 34 is preferably 200 μm to 700 μm. More preferably, they are 200 micrometers-500 micrometers, More preferably, they are 200 micrometers-300 micrometers.

  Furthermore, when making the puncture hole formed when puncturing the needle-like body "the length of the puncture hole to reach the dermis", the height H of the protrusion 34 should be in the range of 200 μm to 500 μm. preferable. Further, when the puncture hole formed when puncturing the needle-like body is "the length by which the puncture hole reaches the epidermis", the height H of the protrusion 34 may be in the range of 200 μm to 300 μm. preferable.

  The width D of the projection 34 is, as shown in FIG. 3C, the largest length among the lengths of the projection 34 in contact with the base 38 when the projection 34 is projected parallel to the base surface. is there. For example, when the protrusion 34 is conical, the diameter of the circle of the surface in contact with the protrusion 34 and the base 38 is the width D. When the protrusion 34 is a square pyramid, the diagonal of the square of the surface in contact with the protrusion 34 and the base 38 is the width D. Further, when the projection 34 is a cylinder, the diameter of the circle of the surface in contact with the projection 34 and the base 38 is the width D. When the protrusion 34 is a square prism, the diagonal of the square of the surface in contact with the protrusion 34 and the base 38 is the width D.

  The width D of the protrusion 34 is preferably 1 μm to 300 μm, more preferably 1 μm to 100 μm, in consideration of how deep the puncture hole formed when puncturing the needle-like body is to be formed in the skin. And more preferably 10 μm to 100 μm.

  The aspect ratio A defined by A = H / D using the length H and the width D of the protrusion 34 is preferably 1 to 10.

  The tip angle θ of the protrusion 34 is preferably 5 ° to 30 °, and more preferably 10 ° to 20 ° when the protrusion 34 has a tip angle like a cone shape and penetrates the stratum corneum. In addition, as shown in FIG.3 (c), tip angle (theta) points out the largest thing among angles (apex angle) at the time of projecting the projection part 34 in parallel with a base surface, as shown in FIG.3 (c).

  Using the original plate 32 manufactured as described above, as shown in FIG. 2C, the projection forming surface of the original plate 32 is filled with the intaglio-forming material and hardened to form the intaglio layer 36.

  A water repellent resin is used as the intaglio-forming material. Examples of the water repellent resin include silicone resin and fluorine resin.

  When the original plate 32 is filled with the intaglio-forming material, a frame may be appropriately provided around the original plate 32 so that the material does not spill from the original plate 32.

  Further, when forming the intaglio layer 36, a plurality of original plates 32 are prepared in advance, arranged in a plane without gaps so that the projections are on the same surface, and filled with the intaglio forming material. Compared with the intaglio layer 36 formed from one original plate 32, the intaglio layer 36 having a larger area in the planar direction can be formed.

  Next, the intaglio layer 36 is peeled off from the original plate 32 (FIG. 2 (d)).

  Here, in order to facilitate peeling of the intaglio layer 36 from the original plate 32, release treatment is performed using a release agent such as a fluorine-based coating agent before filling the intaglio-forming material into the original plate 32. Is preferred.

  Further, as shown in FIG. 2E, the groove 14 is formed on the surface of the intaglio layer 36 on the concave 12 side using a processing machine such as a cutting tool, and the intaglio 10 is manufactured.

  As a method of forming the groove portion 14, a known method can be appropriately used according to a desired groove shape. For example, cutting may be mentioned.

  The shape, size, arrangement, and the like of the groove portion 14 are not limited as long as the groove portion 14 is formed in the intaglio so that the contraction of the needle-like body forming aqueous solution can be suppressed in the drying step (fourth step) of the needle-like body forming aqueous solution. However, as one embodiment of the present invention, it can be defined as follows.

  The cross-sectional shape of the groove portion 14 can be any shape such as square, rectangular, triangular, semicircular, semielliptic, and the like. In FIG. 2 (e), the cross-sectional shape of the groove portion 14 is a triangle.

  The width d of the groove portion 14 is preferably 0.1 μm or more, and more preferably 10 μm or more, in order to effectively suppress the contraction of the aqueous solution for forming a needle-like body in the drying step (fourth step). Is preferred. The contraction of the aqueous solution for forming a needle-like body can be prevented as the width d of the groove portion 14 becomes larger, but the use amount of the aqueous solution for forming a needle-like body increases, so the width d is preferably 3 mm or less. Here, the width d of the groove portion 14 refers to the length of the opening in the cross section in the direction orthogonal to the extending direction of the groove portion 14 as shown in FIG. In FIG. 2E, the width d of the groove 14 is the length of one side of the triangle located in the opening.

  The depth h of the groove portion 14 is preferably 0.1 μm or more, and more preferably 10 μm or more in order to effectively suppress the contraction of the aqueous solution for forming a needle-like body in the drying step (fourth step). preferable. Although the depth h of the groove portion 14 can prevent the contraction of the aqueous solution for forming a needle-like body as the depth h increases, the use amount of the aqueous solution for forming a needle-like material increases, so the depth h is preferably 3 mm or less. Here, the depth h of the groove portion 14 is, as shown in FIG. 2E, from the upper surface of the intaglio 10 to the lowermost point of the groove portion 14 in a cross section in a direction orthogonal to the extending direction of the groove portion 14 Say the distance.

  The length in the stretching direction of the groove portion 14 is preferably more than 300 μm, more preferably 1000 μm or more, and still more preferably 1 cm or more. The groove part 14 may be provided in linear form, and may be provided in curvilinear form. Here, the line shape includes the shape of a broken line or other broken line. In the case where the groove portion 14 is in the form of a broken line, the length in the stretching direction of one unbroken portion constituting the broken line is preferably more than 300 μm, more preferably 1000 μm or more, and still more preferably 1 cm or more. Furthermore, the groove portions 14 may be provided in a lattice shape.

  The groove 14 may be formed until the surface on the concave side of the intaglio layer 36 divided by the groove 14 has a predetermined size so that the effect of suppressing the contraction of the aqueous solution for forming a needle-like body extends to the entire intaglio. For example, when the groove 14 is provided such that the surface on the concave side of the intaglio layer 36 is rectangular and the shape of the surface divided by the groove 14 is also rectangular, the long side of the divided surface is preferably less than 150 mm More preferably, it is 100 mm or less.

  In addition, it is preferable that the groove portion 14 be formed on the surface of the concave portion side of the intaglio layer 36 so that the effect of suppressing the contraction of the aqueous solution for needle-like body formation in the drying step (fourth step) efficiently extends over the entire intaglio. . For example, by forming the grooves 14 so that the surface on the concave side is equally divided by the grooves 14, the ratio of the grooves 14 occupied on the surface of the intaglio layer 36 can be reduced, and the effect of suppressing the contraction can extend to the entire intaglio .

  When a plurality of grooves are provided, the distance between the grooves 14 and 14 is preferably less than 150 mm in order to effectively suppress the contraction of the aqueous solution for forming a needle-like body in the drying step (fourth step). Preferably it is 100 mm or less, More preferably, it is 50 mm or less. Here, when the needle-like body obtained in the fifth step to be described later is cut into a desired size by punching or cutting, the lower limit value of the distance between the groove portion 14 and the groove portion 14 is made equal to or larger than the size. Need to be provided. For example, when the needle-like body to be used finally has a square shape with a side of 30 mm, the distance between the groove portion 14 and the groove portion 14 is set to 30 mm or more.

  In the above description, the groove 14 is formed on the surface of the intaglio layer 36 on the concave 12 side to produce the intaglio 10, but as shown in FIG. The intaglio 10 may be manufactured by providing a groove 14 at the joint by combining two or more pieces.

  In the above, the method for providing the groove portion 14 in the intaglio layer 36 formed from the original plate 32 has been described, but a convex portion corresponding to the groove portion 14 is formed in advance in the original plate 32, filled with the intaglio-forming material and cured. The intaglio 10 may be manufactured.

  FIG. 5 is a perspective view showing an example of the intaglio 10 used in the manufacturing method of the present invention. In the intaglio 10 shown in FIG. 5, the concave portion 12 having a circular opening is formed in a lattice arrangement, and three grooves 14 having a triangular cross-sectional shape are provided in parallel.

<Step of supplying needle-like body formation aqueous solution to intaglio plate (third step)>
Next, as shown in FIG. 1 (b), the aqueous solution 16 for forming a needle-like body prepared in the first step is supplied to the surface on the concave side of the intaglio 10 formed in the second step.

  A frame 18 may be appropriately provided around the intaglio 10 so that the aqueous solution 16 does not spill when the needle-forming aqueous solution 16 is supplied to the intaglio 10. The material of the frame 18 can be appropriately selected from metals, resins, and the like.

  About the supply method of the aqueous solution 16, a well-known method can be selected suitably according to the shape of an intaglio 10, a dimension. For example, as a method of supplying the aqueous solution 16, a spin coating method, an inkjet method, a method using a dispenser, a casting method, or the like can be used. Further, when the aqueous solution 16 is supplied, the environment around the intaglio 10 may be under reduced pressure or under vacuum.

  In addition, in the case where the materials for forming the protrusion and the base of the needle-like body to be manufactured are different, in the first step, an aqueous solution forming the protrusion and an aqueous solution forming the base as the needle-like body forming aqueous solution 16 The aqueous solution which forms a projection part may be supplied to the recessed part 12 of the intaglio 10 first, and then the aqueous solution which forms a base may be supplied.

<Step of Drying Needle-Forming Aqueous Solution (Fourth Step)>
Next, as shown in FIG. 1 (c), the aqueous solution 16 for forming a needle-like body supplied to the intaglio 10 is dried and solidified.

  The drying method can be appropriately selected according to the environment, such as natural drying, bottom surface heating using a hot plate, drying by hot air drying, and the like. However, the drying temperature should be a temperature at which the aqueous solution does not boil. The drying temperature is preferably performed at a temperature of at least 110 ° C. or less. More preferable drying conditions are 40 to 100 ° C. In addition, humidity adjustment may be performed at the time of drying.

  When the groove portion 14 is not provided in the intaglio 10, as the drying progresses and the solvent (water) of the aqueous solution 16 for forming acicular material 16 evaporates, the aqueous solution 16 for forming acicular material may be repelled from the intaglio 10 and contracted. is there. In particular, when using an intaglio 10 having a large area in the planar direction, the degree of shrinkage tends to increase. On the other hand, in the method of the present invention, by providing the groove portion 14 in the intaglio 10, it is possible to suppress the contraction of the aqueous solution 16 for forming the needle-like body during drying.

<Step of Peeling Needle-like Body from Intaglio (Fifth Step)>
Next, the needle-like body formed by drying is peeled off from the intaglio 10 to obtain the needle-like body 20 including the base 26 and the projection 22 on one surface of the base 26 (FIG. 1 (d)).

  As the peeling method, a known method, for example, a physical method such as a mechanical blade or suction can be used.

  The base 26 serves as a base for supporting the projection 22, and usually its surface is flat. The shape and size of the base portion 26 can be appropriately selected in accordance with the application of the needle-like body 20.

  The shape, size, and arrangement of the protrusions 22 on the surface of the base portion 26 are the same as those of the original plate 32 and are as described for the original plate 32 described in the second step.

  FIG. 6 is a perspective view of a needle-like body 20 manufactured using the intaglio 10 shown in FIG. The needle-like body shown in FIG. 6 has projections 22 and convex lines 24 respectively corresponding to the recess 12 and the groove 14 of the intaglio 10 shown in FIG.

  If the depth of the groove 14 provided in the intaglio 10 is greater than the depth of the recess 12, the height of the convex line 24 is higher than the height of the projection 22, so the needle only in the range where the convex line 24 does not exist It can be used as a solid 20. For example, the area including the projection 22 where the convex line 24 does not exist can be cut into a desired size by punching or cutting and used as the needle-like body 20.

  When the depth of the groove 14 provided in the intaglio 10 is smaller than the depth of the recess 12, the height of the convex line 24 is lower than the height of the protrusion 22, so the needle-like body 20 obtained in the fifth step Can be used as it is. However, even in this case, the area of the projection 22 where the convex line 24 of the needle 20 does not exist may be cut into a desired size by punching or cutting.

  As described above, according to the present invention, contraction of the aqueous solution does not occur at the time of drying of the aqueous solution for forming a needle-like body. For this reason, by using an intaglio plate having a large size, it is possible to manufacture a needle-like body having a large area in the planar direction and having almost no unevenness in thickness. Therefore, the productivity of high quality needles can be improved, and the production cost can be reduced.

[Example]
Hereinafter, the present invention will be described in detail using examples and comparative examples, but the present invention is not limited to this embodiment.

<Production of Needles>
Example 1
Needles were produced by the following method.
(1) First, the silicon substrate was precision machined to arrange 1,600 grids of 40 rows and 40 rows with 1 mm intervals between the projections of the square pyramid (bottom surface 38 μm × 38 μm, height 120 μm). An original plate of a 50 mm × 50 mm needle-like body was formed. At this time, the distance W between the projection 34 and the projection 34 is 962 μm.
(2) Next, the original plate of the needle-like body formed in (1) was subjected to release treatment using a fluorine-based coating agent.
(3) Align the mold-released original plate of (2) in 5 rows and 5 columns without gaps, pour silicone (Momentive Performance Materials, TSE 3032), cure at 150 ° C, and make an intaglio plate of 250 mm × 250 mm in size The layers were made.
(4) In the intaglio layer produced in (2), using a chisel, grooves having an equilateral triangle with a cross section of 1 mm were formed in a grid of 50 mm intervals to produce an intaglio.
(5) A frame made of silicone was provided on the outer periphery of the intaglio plate of (4), and a 5% by mass aqueous solution of sodium chondroitin sulfate was poured so that the depth (flat portion of the intaglio plate from the liquid surface) became 5 mm.
(6) The intaglio plate of (5) was placed on a hot plate set at 80 ° C. for 5 hours, dried, and the water was evaporated.
(7) The needle-like body was peeled off from the intaglio.

Example 2
An original plate of needle-like body is arranged without gaps in 2 rows and 3 columns to obtain an intaglio layer of 100 mm × 150 mm in size, and grooves are formed in a grid at intervals of 50 mm. Needles were made.

[Example 3]
An original plate of needle-like body is arranged without gaps in two rows and three columns to obtain an intaglio layer 100 mm × 150 mm in size, and two grooves are provided in parallel at 50 mm intervals in a direction perpendicular to the 150 mm side of the intaglio layer. Needles were produced in the same manner as in Example 1 except for the exception.

Comparative Example 1
In Example 1, a needle-like body was produced without providing a groove on the intaglio.

Comparative Example 2
The needle plate masters were arranged in two rows and three columns without gaps to obtain an intaglio layer having a size of 100 mm × 150 mm, and the needles were formed in the same manner as in Example 1 except that no groove was formed in this. Made.

<Result>
In Examples 1 to 3, the needle-like body could be produced so as to spread over the entire surface of the intaglio. On the other hand, in Comparative Examples 1 and 2, the aqueous solution of sodium chondroitin sulfate contracts as drying progresses, and does not spread over the entire surface of the intaglio plate, and a needle-like body having an area smaller than that of the intaglio plate was obtained.

  The needles produced by the method of the present invention can be used in various fields requiring fine needles. For example, it can be used in various ways as a MEMS device, an optical member, a sample jig, a medicine, or a part used in medical, cosmetic, cosmetic applications.

DESCRIPTION OF SYMBOLS 10 ... Intaglio 12 ... Concave part 14 ... Groove part 20 ... Needle-like body 22 ... Protrusion part 26 ... Base part

Claims (2)

A method of manufacturing a needle-like body, comprising: a base; and a protrusion on one surface of the base,
Providing an aqueous solution for forming a needle-like body containing a material for forming a needle-like body;
Preparing an intaglio plate formed of a material containing a water repellent resin, having a recess corresponding to the shape of the protrusion;
Supplying the needle-forming aqueous solution to the intaglio plate;
Drying the aqueous solution for forming a needle-like body supplied to the intaglio;
Peeling the needle-like body formed by the drying from the intaglio plate;
Including
A linear groove is provided on the surface on the concave side of the intaglio (except that the groove only surrounds the entire area where the concave is formed), and is divided by the groove The method for producing a needle-like body , wherein the long side of the rectangular is 50 mm or more and less than 150 mm when the shape of the surface on the concave side of the intaglio is rectangular .   The method for producing a needle-like body according to claim 1, wherein the water repellent resin is a silicone resin or a fluorine resin.

Images