JP6060547B2 - Manufacturing method of needle-shaped body - Google Patents

Description

  The present invention relates to a method for manufacturing a needle-shaped body.

  The percutaneous absorption method, which is a method of injecting a delivery product such as a drug from the skin and administering the delivery product into the body, is used as a method capable of easily administering the delivery product without causing pain to the human body. . In the field of transdermal administration, a method of perforating the skin using a fine needle (microneedle) on the order of μm and administering a drug into the skin has been proposed (see Patent Document 1).

  The shape of the fine needle-like body used at this time is required to have a sufficient fineness and tip angle for piercing the skin and a sufficient length for allowing the drug solution to penetrate subcutaneously, The diameter is several μm to several hundred μm (specifically, for example, about 1 μm to 300 μm), and the length is specifically several tens of μm to several hundred μm (specifically, for example, about 20 μm to 1000 μm). Is desirable.

  In addition, the material constituting the acicular body is preferably a material that does not adversely affect the human body even if the damaged acicular body remains in the body, such as chitin and chitosan. A biocompatible material is suitable (see Patent Document 2).

  In addition, as a method for manufacturing the needle-like body described above, it has been proposed to create an original plate using cutting, form a duplicate plate, and perform transfer processing molding (see Patent Document 3).

JP-A-48-93192 International Publication No. 2008/020632 Pamphlet International Publication No. 2008/013282 Pamphlet

  The needle-like body that punctures the skin may be required to have a specification that is required not to peel from the skin after puncturing.

  Then, this invention is made | formed in order to solve the above-mentioned subject, and it aims at providing the manufacturing method of the acicular body which manufactures the acicular body which can suppress peeling from skin after puncture. .

In order to solve the above-mentioned problem, the invention according to claim 1 is a method of manufacturing a needle-like body having a needle-like protrusion on one surface of a base material, and the needle-like surface is provided on one side of the base material. A needle-shaped original plate preparation step for preparing a needle-shaped original plate provided with a protrusion, a microcapsule dispersion is applied to the protrusion-forming surface of the needle-shaped original plate , and a micro-capsule dispersion is applied to the protrusion-forming surface of the needle-shaped original Arranging a capsule, forming a microprojection on the surface of the projection of the needle-shaped original plate, forming a microplate from the needle-shaped original plate on which the microprotrusion is formed, and producing a replica plate, By filling the replica plate with the needle-shaped body forming material and peeling the needle-shaped body forming material, the needle-shaped body is provided with a needle-like projection on one surface of the base material, and the projection surface has a micro-projection. The needle-shaped body manufacturing method is characterized by comprising a body and a transfer molding step to be manufactured.
Further, the invention according to claim 2 includes a surface modification step for modifying the surface of the projection of the needle-shaped body original plate before arranging the microcapsule on the surface of the projection of the needle-shaped body original plate. The needle-shaped body manufacturing method according to claim 1, wherein the needle-shaped body manufacturing method is provided.

  In the method for producing a needle-shaped body of the present invention, a microcapsule having high wettability is applied to the surface of a needle-shaped body original plate. As a result, the microcapsules remain in a granular shape on the surface of the needle-shaped body original plate, and a large amount of microprojections can be formed on the surface of the needle-shaped body. Then, the needle-shaped body original plate in which the microcapsules are arranged on the grains is duplicated to produce a needle-shaped body, so that the shape having the formed micro-projection site is formed in the skin when the needle-shaped body is punctured into the skin. The needle-like body can be prevented from being peeled from the skin after puncturing.

FIG. 1 is a perspective view of a needle-shaped body of the present invention. FIG. 2 is an enlarged perspective view of the protrusion of the needle-shaped body of the present invention. FIG. 3 is a schematic cross-sectional view of the protrusion of the needle-shaped body of the present invention. FIG. 4 is a schematic cross-sectional view (explanatory drawing) of the method for producing a needle-shaped body of the present invention. FIG. 5 is a schematic cross-sectional view (explanatory drawing) of the method for manufacturing a needle-shaped body of the present invention.

The acicular body of the present invention will be described.
The perspective view of the needlelike object of the present invention of Drawing 1 was shown. The needle-like body 1 of the present invention includes a needle-like protrusion 12 on a base material 11. In the needle-like body 1 of the present invention, the base 11 may have one protrusion, and the base 11 may have a plurality of protrusions. When a plurality of protrusions are provided, the protrusions may be provided in an array. Here, the “array shape” indicates a state in which the protrusions are arranged as shown in FIG. 1, for example, a lattice arrangement, a close-packed arrangement, a concentric arrangement, a random arrangement, etc. Including patterns.

  The shape of the protrusion of the needle-like body is preferably a cone shape. Specifically, cone shapes such as a cone and a pyramid are exemplified. In addition, a pencil shape in which a cone shape and a columnar shape such as a cylinder or a prism are combined (the body portion is a column shape and the tip portion is a cone shape) can be selected. Further, as the shape of the needle-like portion of the needle-like body, a truncated cone shape or a truncated pyramid shape can be selected. In addition, as the shape of the needle-like portion of the needle-like body, a columnar shape can be selected.

  FIG. 2 shows an enlarged perspective view of the protrusion of the needle-like body of the present invention. The projection 12 of the needle-like body of the present invention has a microprojection 13 on the surface. By providing the microprojections on the surface of the projecting portion, it is possible to suppress the needle-like body from being moored in the skin when the needle-like body is punctured into the skin and peeling off from the skin after the puncture. In addition, a needle-like body having a large amount of microprojections on the surface of the protrusion can hold a large amount of delivery on the surface of the protrusion when a delivery product such as a medicine is provided on the surface of the needle-like body.

  FIG. 3 shows a schematic cross-sectional view of the protrusion of the needle-shaped body of the present invention. In the needle-like body of the present invention, it is preferable that the dimensions of the protrusion 12 have a thickness and a length suitable for forming a puncture hole in the skin. Specifically, the height H of the needle-like portion shown in FIG. 3 is preferably in the range of 10 μm or more and 1000 μm or less. The height H of the protrusion is a distance from the base material 11 to the tip of the protrusion 12. The height H of the protrusion is preferably determined in consideration of how deep the puncture hole formed when the needle-like body is punctured within the above range is formed in the skin.

  In particular, when the puncture hole formed when the needle-like body is punctured is held in “in the stratum corneum”, the height H of the protrusion of the needle-like body is in the range of 10 μm to 300 μm, more preferably 30 μm to 200 μm. It is desirable to be inside.

  Further, when the puncture hole formed when the needle-like body is punctured is kept to “the length that penetrates the stratum corneum and does not reach the nerve layer”, the height H of the protrusion of the needle-like body is 200 μm or more and 700 μm. In the following, it is more preferable that the thickness be in the range of 200 μm to 500 μm, and more preferably 200 μm to 300 μm.

  Further, when the puncture hole formed when the needle-like body is punctured is “the length that the puncture hole reaches the dermis”, the height H of the protrusion of the needle-like body is in the range of 200 μm to 500 μm. It is preferable that When the puncture hole formed when the needle-like body is punctured is “the length that the puncture hole reaches the epidermis”, the height H of the projection of the needle-like body is in the range of 200 μm or more and 300 μm or less. It is preferable.

  Moreover, it is preferable that the width | variety D of a needle-shaped part exists in the range of 1 micrometer or more and 300 micrometers or less. The width D of the needle-like portion is preferably determined in consideration of the depth of the puncture hole formed when the needle-like body is punctured within the above range. The width D of the protrusion is the maximum length among the lengths of the needle-shaped portions in contact with the base when the protrusion is projected in parallel with the substrate surface. For example, when the needle-like part has a conical shape, the diameter of the circle of the surface in contact with the needle-like part and the base body is the width D. When the needle-like part is a regular quadrangular pyramid, the square diagonal line of the surface in contact with the protrusion and the base is the width D.

  In addition, the aspect ratio A of the needle-shaped part is preferably in the range of 1 or more and 10 or less. The aspect ratio A is defined by A = H / D using the length H and width D of the protrusion.

  Further, when the needle-like body includes a plurality of protrusions, the heights of the protrusions may or may not be the same. When the lengths of the protrusions are different, for example, (1) when the protrusions are high only on the outer periphery of the array, there is an effect that the curved surface can be suitably contacted. In addition, (2) by providing a low protrusion only on the outer periphery of the array, there is an effect that the mechanical strength of the microneedles on the outer periphery that is easily damaged can be reinforced. Further, the pitch dimension, which is the distance between the protrusions, may be appropriately designed and determined according to the specifications.

  In addition, when the shape of the needle-like body is a shape in which a plurality of protrusions are erected on the base material, the fine protrusions are also formed on the base material portion between the protrusions. At this time, the microprotrusions on the base material increase the surface area of the entire needle-like body, and depending on the delivery product such as pharmaceuticals, the delivery product on the bottom surface is also transmitted to the tip of the needle-like body through the side surface of the needle-like body. Therefore, it is possible to increase the amount of the delivered product and the effective use efficiency.

  In addition, in FIG. 3, it is an array structure in which the needle-shaped projection part which is a cone-shaped cone of the shape which made the surface which touched the base material the bottom face, and made the perpendicular | vertical direction apex, and a board | substrate and a protrusion bottom face are integrally molded. Has been. Although FIG. 3 shows an example of the needle-like body 1 in which the base material 11 and the protrusion 12 are integrally formed, the base material and the protrusion may be made of different types of materials.

  Next, the manufacturing method of the acicular body of this invention is demonstrated. 4 and 5 show schematic cross-sectional views (descriptive drawings) of the method for producing the needle-shaped body of the present invention.

  The needle-shaped body manufacturing method of the present invention includes a needle-shaped body original plate preparation step for manufacturing a needle-shaped body original plate having a needle-shaped protrusion on one surface of a substrate, and a protrusion-forming surface of the needle-shaped body original plate A micro-projection forming step for forming a micro-projection on the surface of the projection of the needle-shaped body original plate, and a replica plate-preparing step for preparing a duplicate plate from the needle-shaped original plate on which the micro-projections are formed; The replica plate is filled with a needle-like body forming material and the needle-like body forming material is peeled off, whereby a needle-like protrusion is provided on one surface of the substrate, and a minute protrusion is provided on the surface of the protrusion. It comprises a needle-shaped body and a transfer molding process to be produced.

[Needle body forming process]
First, the acicular body original plate 21 having acicular protrusions on one surface of the substrate is produced (FIG. 4A). As a method for producing the needle-shaped body precursor, a needle-shaped body precursor manufacturing method using a known fine processing technique can be used as appropriate. For example, (1) a needle-shaped original plate manufacturing method using fine machining such as grinding or cutting on a plate-shaped substrate, and (2) a needle-shaped original plate using a fine processing technique such as a lithography method or an etching method. Examples include production methods. The plate-shaped substrate material for producing the needle-shaped body original plate can be appropriately selected according to the processing method. For example, substrate materials such as carbon, ceramics, silicon, quartz, metal, and resin may be used.

[Surface modification process]
Before the microcapsules are arranged on the protrusion-forming surface of the needle-shaped body original plate, it is preferable to provide a surface modification step for modifying the surface of the needle-shaped body original plate on which the protrusion-forming portion is formed (FIG. 4B). . Although the surface modification step is an optional step, by providing the surface modification layer 22 by surface modification, when the microcapsules are disposed on the projection forming surface of the substrate precursor using the microcapsule dispersion described later. The fluid microcapsules can be agglomerated on the surface of the needle-shaped original plate having high wettability.

  As the surface modification method, (1) plasma surface modification method, (2) surface modification method using ozone gas, and (3) a surface modification layer having high wettability with respect to microcapsules is formed on the original needle body. A method of forming, (4) a method of forming fine irregularities of submicron order on the surface of the needle-like body by a fine processing technique, and the like can be used.

  When the surface modified layer is formed on the acicular body original plate, the surface modified layer may be hydrophilic, and the microprojection may be an aqueous microcapsule dispersion. When the microprojection is a water-based microcapsule dispersion, the surface modified layer exhibits hydrophilicity, whereby the surface of the acicular body original plate can be made highly wettable with respect to the microcapsules.

  In the case of applying the material to be the surface modification layer to the needle body original plate, as a method for applying the surface modification layer, a known application method suitable for a three-dimensional shape may be used. Specific examples of the coating method include a dip coating method, a spray coating method, and a potting coating method. In particular, the dip coating method can ensure the fluidity of the surface modification layer on the surface of the needle-like body original plate, and can preferably be applied to the entire surface of the needle-like body. In addition, as a coating material used as a surface modification layer, polyethylene glycol and polyvinyl alcohol can be used.

[Microprojection forming process]
Next, a microcapsule that becomes a minute protrusion is disposed on the protrusion-forming surface of the needle-shaped body original plate. However, the microcapsule in the present invention refers to a micro container having a micrometer size. That is, a capsule whose diameter is in the micrometer region when it is considered as a sphere.

  There are many methods for producing microcapsules, which can be broadly classified into chemical methods using chemical reactions, physicochemical methods using physicochemical changes, and physical methods mainly using physical or mechanical operations. .

  There are many examples of using microcapsules, but the following is a summary of the functions used. For example, there are pressure-sensitive copying paper, pressure measurement sheets, pressure-sensitive adhesives, liquid crystal display materials, etc. that use the function to protect the core material, which is the material in the capsule, from the external environment. Examples of products that use the function of regulating the release rate to the environment include sustained-release pharmaceuticals and sustained-release agrochemicals. Furthermore, their use in artificial cells and artificial organs fulfills both functions simultaneously.

  The material forming the wall film of the microcapsule is mainly natural and synthetic polymers, but there is no necessity to be a polymer, and waxes and inorganic compounds can also be used as the wall material.

  The microcapsule has high wettability with respect to the surface of the acicular body original plate, and remains in a granular form on the surface of the acicular body original plate. When a microcapsule is applied to a highly wettable surface, the microcapsule is easily spread uniformly. The present invention utilizes the above knowledge. In addition, although the average particle diameter of the microcapsule used with the manufacturing method of the acicular body of this invention is based also on the side area and shape of an acicular body original plate, it is desirable to exist in the range of 0.5-100 micrometers. The average particle diameter can be obtained as an average value obtained by measuring the maximum diameter of the 20 microcapsules with an optical microscope or an electron microscope. In addition, the plurality of microprotrusions that are formed are randomly arranged with no regularity between the microprotrusions.

  In arranging the microcapsules to be microprojections on the projection forming surface of the needle-shaped original plate, prepare a microcapsule dispersion and apply the microcapsule dispersion 23 to the projection forming surface of the needle-shaped original plate. (FIG. 4C) and can be formed by drying (FIG. 4D).

  When the microcapsule dispersion is applied to the needle plate precursor, a known application method suitable for a three-dimensional shape may be used as the method for applying the microcapsule dispersion.

  Specific examples of the coating method include a dip coating method, a spray coating method, and a potting coating method. In particular, the dip coating method can ensure the fluidity of the microcapsules on the surface of the needle-shaped body original plate, and can preferably be applied to the entire surface of the needle-shaped body.

  The microcapsule dispersion includes various water-soluble synthetic resins such as polyvinyl alcohol (PVA) solution and polyethylene glycol (PEG) solution, and various resin-containing organic solvent solutions such as phenol resin (PF) solution and epoxy resin (EP) solution. Etc. may be used.

  Further, after applying the microcapsule dispersion liquid 23, the microcapsule dispersion liquid on the needle-like body may be dried by heating. By the heat treatment, the solvent of the microcapsules can be volatilized, and the microcapsules having fluidity can be agglomerated on the surface of the acicular original plate having high wettability.

  As described above, an acicular body original plate having microprojections 24 in which microcapsules are arranged on the surface of the projection is produced.

[Copying plate making process]
Next, a duplicate plate 25 is produced from the needle-shaped body original plate (FIGS. 5E and 5F).
The duplicate plate 25 is an intaglio plate corresponding to the shape of the acicular body original plate.
By making a duplicate plate from the needle plate precursor and performing transfer molding using the replica plate, the microprojections made of the desired needle-like body forming material are regular without being restricted by the characteristics required of the microcapsule. A needle-like body having a randomly arranged shape can be produced.
In addition, since a needle-like body in which a plurality of minute protrusions are randomly arranged without regularity can be manufactured from the same duplicate plate, the production cost can be reduced and the productivity can be increased. .

  The material of the duplicate plate 25 can be selected in consideration of the shape following ability to function as the duplicate plate, the transfer property in the transfer processing molding described later, the durability, and the release property.

Further, as a method for producing a duplicate plate, a known shape transfer method may be appropriately used depending on the material of the duplicate plate. For example, (1) When nickel is used as the material of the duplicate plate, electroforming plating method, PVD method, etc. (2) When a thermosetting resin (eg, epoxy resin, silicone resin, etc.) is used as the material of the duplicate plate Alternatively, a method may be used in which a molten resin is allowed to flow into the needle-shaped body original plate, the resin is cured, and the resin is peeled off from the original plate.
At this time, for example, (1) peeling by physical peeling force, (2) selective etching, and the like can be used as a method for peeling the duplicated plate and the acicular body original plate.

[Transfer molding process]
Next, the obtained replica plate 25 is filled with the needle-like body forming material 26 (FIG. 5G), and the needle-like body-forming material is peeled off, whereby a needle-like protrusion is formed on one surface of the substrate. And a needle-like body 26 having minute protrusions on the surface of the protrusion (FIG. 5 (h)).

  Moreover, when performing the transfer molding process using a replication plate, it is preferable that the acicular body forming material is a biocompatible material. By using a biocompatible material, even if problems such as breakage or chipping occur at the time of puncture, adverse effects on the human body can be suppressed. In particular, the needle-shaped body of the present invention is likely to remain in the body because the microprojections are anchored to the skin, and the effect of using a biocompatible material is great.

  As a method of filling the replica plate with the needle-shaped body forming material, a known filling method may be used as appropriate. Examples of the filling method include an imprint method, a hot embossing method, an injection molding method, an extrusion molding method, and a casting method.

  Examples of the biocompatible material as the needle-shaped body forming material for forming the needle-shaped body include medical silicone, resins such as polylactic acid, polyglycolic acid, and polycarbonate. When these materials are used, the replica is filled with the needle-shaped body forming material by melting the resin, and the needle-shaped body is formed by cooling.

  In addition, the biocompatible material as the needle-forming material for forming the needle-shaped body includes alginate, curdlan, chitin, chitosan, glucomannan, polymalic acid, hyaluronic acid, collagen, collagen peptide, hydroxypropylcellulose, gelatin And water-soluble polymers such as When these materials are used, the needle-shaped body is formed by dissolving or dispersing the material in a solvent such as water, liquefying the material, filling the duplicate plate, and removing the solvent by heating or the like.

  Further, in order to improve the peelability of the replica plate and the needle-shaped body forming material, a release layer for increasing the mold release effect may be formed on the surface of the replica plate before filling the needle-shaped body forming material. Good. As the release layer, for example, a widely known fluorine-based resin can be used. Moreover, as a formation method of a mold release layer, thin film formation methods, such as PVD method, CVD method, a spin coat method, a dip coat method, can be used suitably.

  As a method for peeling the duplicated plate and the acicular body original plate, for example, peeling by physical peeling force can be used.

  As described above, the present invention can be implemented. In addition, the manufacturing method of the acicular body of this invention is not limited to the said embodiment, The other well-known method which can be guessed in each process shall also be included.

  Examples will be described below.

<Example 1>
First, the acicular body original plate 21 was prepared by using micromachining on the substrate.
A carbon plate having a thickness of 5 mm was prepared as a substrate material for producing the needle-shaped body original plate 21. Further, as fine machining, a two-blade ball end mill having a ball radius of 0.01 mm was used. The above-mentioned ball end mill is attached as a cutting tool to an NC control milling machine having an XYZ axis, and a conical projection is formed by increasing the cutting amount as the tool revolves from the center of the needle-like body to the outer peripheral direction. Was processed.
The setting conditions of the ball end mill were a rotational speed of 50000 rpm and a feed rate of 0.1 mm / s. The diameter of the bottom surface of the cone formed by machining was designed to be 280 μm, the pitch was 500 μm, and the machining depth was 450 μm. In addition, the portion without the needle-like body was cut uniformly at a depth of 450 μm, so that the protrusion vertex was highest on the substrate.

  The needle-shaped body original plate produced as described above was observed with a scanning electron microscope. As a result, it was confirmed that a conical acicular original plate 21 having a root width of 280 μm and a height of 445 μm and a tip angle of 35 ° could be produced.

Next, the surface modification layer 22 was formed on the surface of the needle-shaped body original plate 21 (FIG. 3B).
At this time, the surface modification layer 22 formed an oxide film by oxygen plasma treatment. When the contact angle of the surface modified layer 22 to water was measured, it was 20 °. Therefore, it was confirmed that the surface of the acicular body original plate 21 shows hydrophilicity.

Next, the microcapsule dispersion liquid 23 was applied to the acicular body original plate 21 on which the surface modification layer 22 was formed (FIG. 4C).
At this time, a microcapsule dispersion (Matsumoto Microsphere: manufactured by Matsumoto Yushi Seiyaku Co., Ltd.) in which liquid low-boiling hydrocarbons were wrapped in a thermoplastic polymer shell (shell) was used.
Further, as a coating method, a dip coating method was used, and the lifting speed was set to 100 mm / min. Further, the microcapsules 7 remained in a granular form on the needle-shaped body original plate 5, and the particle diameter thereof was 3 μm to 10 μm, and the average particle diameter was 6 μm.

  Next, the needle-shaped body original plate coated with the microcapsule dispersion liquid 23 was subjected to heat treatment and naturally cooled at room temperature (FIG. 4D). The heat treatment was performed at 100 ° C. for 1 hour using a clean oven. As described above, an acicular body original plate provided with the fine protrusions 24 was produced.

  When the produced acicular body was observed with a scanning electron microscope, an infinite number of microprojections having a width and a height of about 3 μm to 10 μm were observed on the side surface of the acicular body. It was confirmed that a needle-like body provided with

  Next, the replica plate 9 was produced using the needle-like body original plate 21 on which the plurality of minute protrusions 24 were formed as an original plate, and was subjected to transfer processing molding. This will be specifically described with reference to FIG.

  The acicular body original plate 21 was filled with an epoxy resin as a replica plate material 25, and was subjected to thermosetting treatment at 100 ° C. for 1 hour using a clean oven (FIG. 5E).

  Next, the acicular body original plate 21 and the cured duplication plate material 25 were peeled off to produce a duplication plate 25 made of an epoxy resin (FIG. 5 (f)).

  Next, the replica plate 25 was filled with the needle-shaped body forming material 26 by using an imprint method (FIG. 5G). At this time, polyglycolic acid, which is a biocompatible material, was used as the needle-like body forming material 26.

  Next, the duplicate plate 25 and the needle-shaped body forming material 26 were peeled off to produce the needle-shaped body 26 of the present invention made of the needle-shaped body forming material 10 (FIG. 4G).

  When the needle-like body 1 of the present invention made of the needle-like body-forming material 16 was observed with a scanning electron microscope, it had a root diameter of 280 μm, a height of 445 μm, a tip angle of 35 °, and a width and height. It was confirmed that an infinite number of minute protrusions having a length of about 2 to 5 μm were formed.

<Comparative Example 1>
Except for not performing the microcapsule placement step, a needle-like body having no microprotrusions on the surface was produced in the same manner as in the method for producing a needle-like body of <Example 1>.

  After the obtained needle-like body of <Example 1> and the protrusion of the needle-like body of <Comparative Example 1> were punctured into the artificial skin, it was confirmed whether or not the needle-like body was easily pulled out. It was confirmed that the needle-like body of <Example 1> was less likely to be pulled out than the needle-like body of 1> and could be prevented from peeling off from the skin.

  The method for producing a needle-shaped body of the present invention can be applied not only to medical treatment but also to various fields that require a fine needle-shaped body, such as MEMS devices, optical members, drug discovery, cosmetics, and cosmetic applications. It is also useful as a method for producing fine needles to be used.

DESCRIPTION OF SYMBOLS 1 Acicular body 11 Base material 12 Protrusion part 13 Microprotrusion 21 Acicular body original plate 22 Surface modification layer 23 Microcapsule dispersion liquid 24 Microprotrusion 25 Duplicate plate 26 Acicular body formation material / acicular body

Claims (2)

A method for producing a needle-like body having a needle-like protrusion on one surface of a substrate,
A needle-shaped original plate preparation step for preparing a needle-shaped original plate provided with a needle-like protrusion on one surface of the substrate;
A microcapsule dispersion is applied to the protrusion-forming surface of the needle-shaped original plate, the microcapsules are arranged on the protrusion-forming surface of the needle-shaped original plate, and minute protrusions are formed on the surface of the needle-shaped original plate. Microprojection forming process,
A replica plate making step of preparing a replica plate from the needle-shaped body original plate on which the microprojections are formed;
A needle provided with a needle-like protrusion on one surface of the substrate and a micro-protrusion on the surface of the protrusion by filling the replica plate with the needle-like member-forming material and peeling the needle-like member-forming material. A method for producing a needle-like body, comprising: a shaped body and a transfer molding step for producing the shaped body.   The surface modification process of modifying the surface of the protrusion-forming surface of the needle-shaped body precursor plate before arranging the microcapsules on the surface of the needle-shaped body original plate. A method for producing a needle-like body.

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