JP6420281B2 - タンパク質解析用固相担体及びその製造方法 - Google Patents

タンパク質解析用固相担体及びその製造方法 Download PDF

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Publication number
JP6420281B2
JP6420281B2 JP2016132569A JP2016132569A JP6420281B2 JP 6420281 B2 JP6420281 B2 JP 6420281B2 JP 2016132569 A JP2016132569 A JP 2016132569A JP 2016132569 A JP2016132569 A JP 2016132569A JP 6420281 B2 JP6420281 B2 JP 6420281B2
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Prior art keywords
antibody
protein
immobilized
amount
solid phase
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Japanese (ja)
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JP2018004476A5 (https=
JP2018004476A (ja
Inventor
剛史 大場
剛史 大場
紗也加 野地
紗也加 野地
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Kao Corp
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Kao Corp
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Priority to JP2016132569A priority Critical patent/JP6420281B2/ja
Priority to PCT/JP2017/024368 priority patent/WO2018008596A1/ja
Priority to US16/314,477 priority patent/US20190162720A1/en
Publication of JP2018004476A publication Critical patent/JP2018004476A/ja
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/26—Synthetic macromolecular compounds
    • B01J20/262—Synthetic macromolecular compounds obtained otherwise than by reactions only involving carbon to carbon unsaturated bonds, e.g. obtained by polycondensation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
    • C07K17/02—Peptides being immobilised on, or in, an organic carrier
    • C07K17/08—Peptides being immobilised on, or in, an organic carrier the carrier being a synthetic polymer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/44—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
JP2016132569A 2016-07-04 2016-07-04 タンパク質解析用固相担体及びその製造方法 Active JP6420281B2 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2016132569A JP6420281B2 (ja) 2016-07-04 2016-07-04 タンパク質解析用固相担体及びその製造方法
PCT/JP2017/024368 WO2018008596A1 (ja) 2016-07-04 2017-07-03 タンパク質解析用固相担体及びその製造方法
US16/314,477 US20190162720A1 (en) 2016-07-04 2017-07-03 Solid phase support for protein analysis and method for producing same

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2016132569A JP6420281B2 (ja) 2016-07-04 2016-07-04 タンパク質解析用固相担体及びその製造方法

Publications (3)

Publication Number Publication Date
JP2018004476A JP2018004476A (ja) 2018-01-11
JP2018004476A5 JP2018004476A5 (https=) 2018-05-24
JP6420281B2 true JP6420281B2 (ja) 2018-11-07

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JP2016132569A Active JP6420281B2 (ja) 2016-07-04 2016-07-04 タンパク質解析用固相担体及びその製造方法

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US (1) US20190162720A1 (https=)
JP (1) JP6420281B2 (https=)
WO (1) WO2018008596A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7120547B2 (ja) * 2019-02-22 2022-08-17 国立研究開発法人理化学研究所 物質固定化剤、及び当該物質固定化剤を用いた物質固定化方法
CN114130377A (zh) * 2021-12-14 2022-03-04 无锡创谱生物科技有限公司 一种亲和层析填料、其制备方法及用途

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05297000A (ja) * 1992-04-17 1993-11-12 S R L:Kk インスリン抗体の測定方法
US6406921B1 (en) * 1998-07-14 2002-06-18 Zyomyx, Incorporated Protein arrays for high-throughput screening
US7329536B2 (en) * 2003-06-05 2008-02-12 Oakland University Piezoimmunosensor
KR100920729B1 (ko) * 2007-10-01 2009-10-07 한국생명공학연구원 펩타이드 혼성체를 사용한 배향성이 조절된 항체단분자막의 제조방법
JP2009240235A (ja) * 2008-03-31 2009-10-22 Sekisui Chem Co Ltd 融合タンパク質、遺伝子、融合タンパク質の製造方法、並びに、抗原タンパク質の検出方法
WO2011089903A1 (en) * 2010-01-25 2011-07-28 Panasonic Corporation A method for immobilizing protein a on a self-assembled monolayer

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Publication number Publication date
WO2018008596A1 (ja) 2018-01-11
JP2018004476A (ja) 2018-01-11
US20190162720A1 (en) 2019-05-30

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