JP6385356B2 - Dnaの検出方法 - Google Patents
Dnaの検出方法 Download PDFInfo
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- JP6385356B2 JP6385356B2 JP2015543920A JP2015543920A JP6385356B2 JP 6385356 B2 JP6385356 B2 JP 6385356B2 JP 2015543920 A JP2015543920 A JP 2015543920A JP 2015543920 A JP2015543920 A JP 2015543920A JP 6385356 B2 JP6385356 B2 JP 6385356B2
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/4473—Arrangements for investigating the separated zones, e.g. localising zones by electric means
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
- G01N27/3278—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction involving nanosized elements, e.g. nanogaps or nanoparticles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44791—Microapparatus
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- Health & Medical Sciences (AREA)
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- Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Electrochemistry (AREA)
- Wood Science & Technology (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Nanotechnology (AREA)
- Dispersion Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Hematology (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Description
16 先端部
51 プライマー
52 環状テンプレート
53 単鎖DNA生成物
57 微粒子
Claims (13)
- 少なくとも一対の電極を備える検出装置を使用するDNAの検出方法であって、
(a)前記電極にプライマーを固定し、
(b)単鎖DNAの環状テンプレートを含有する溶液内に前記電極を浸し、前記環状テンプレートをアニールし、RCA法によって単鎖DNA生成物を生成することにより、所定の電圧を印加した前記電極間を伸張したDNAによって橋渡しさせ、
(c)複数の単鎖DNA分子を含む前記電極間を橋渡しするDNAの性質決定を行うこと、
を含むことを特徴とするDNAの検出方法。 - 前記電極の少なくとも一部は金で被覆されている請求項1に記載のDNAの検出方法。
- 前記伸張したDNAによる前記電極間の橋渡しは、定温で行われる請求項1又は2に記載のDNAの検出方法。
- 前記電極間を橋渡しするDNAの性質決定は、前記電極間を橋渡しするDNAの共振周波数に基づいて行われる請求項1〜3のいずれか1項に記載のDNAの検出方法。
- 前記電極間の間隔を所定の周波数で変化させる請求項4に記載のDNAの検出方法。
- 前記電極間を橋渡しするDNAは束になっている請求項1〜5のいずれか1項に記載のDNAの検出方法。
- 前記電極間を橋渡しするDNAは、二本鎖DNA分子を含む束になっている請求項6に記載のDNAの検出方法。
- 前記電極間を橋渡しするDNAの性質決定は、前記電極間を橋渡しするDNAの導電性に基づいて行われる請求項1〜7のいずれか1項に記載のDNAの検出方法。
- 前記電極間を橋渡しするDNAの性質決定は、前記電極間を橋渡しするDNAのリアルタイム計測によって行われる請求項1〜8のいずれか1項に記載のDNAの検出方法。
- 単鎖の相補的DNAが生成されるように、対向する電極には異なるプライマーを固定する請求項1〜9のいずれか1項に記載のDNAの検出方法。
- 複数の電極対に複数のプライマーを固定することによって、複数のDNAの性質決定を行う請求項1〜10のいずれか1項に記載のDNAの検出方法。
- 少なくとも一対の電極を備える検出装置を使用するDNAの検出方法であって、
(a)前記電極にプライマーを固定し、
(b)単鎖DNAの環状テンプレートを含有する溶液内に前記電極を浸し、前記環状テンプレートをアニールし、RCA法によって単鎖DNA生成物を生成することにより、所定の電圧を印加した前記電極間を伸張したDNAによって橋渡しさせ、
(c)前記電極間を橋渡しするDNAに導電性の微粒子を被覆し、
(d)前記電極間を橋渡しするDNAの存在を確認すること、
を含むことを特徴とするDNAの検出方法。 - 複数の電極対に複数のプライマーを固定することによって、複数のDNAの存在を確認する請求項12に記載のDNAの検出方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2013221803 | 2013-10-25 | ||
JP2013221803 | 2013-10-25 | ||
PCT/JP2014/078298 WO2015060417A1 (ja) | 2013-10-25 | 2014-10-24 | Dnaの検出方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JPWO2015060417A1 JPWO2015060417A1 (ja) | 2017-03-09 |
JP6385356B2 true JP6385356B2 (ja) | 2018-09-05 |
Family
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2015543920A Expired - Fee Related JP6385356B2 (ja) | 2013-10-25 | 2014-10-24 | Dnaの検出方法 |
Country Status (6)
Country | Link |
---|---|
US (1) | US10845333B2 (ja) |
EP (1) | EP3064934B1 (ja) |
JP (1) | JP6385356B2 (ja) |
CA (1) | CA2929042A1 (ja) |
IL (1) | IL245222B (ja) |
WO (1) | WO2015060417A1 (ja) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
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US10481158B2 (en) | 2015-06-01 | 2019-11-19 | California Institute Of Technology | Compositions and methods for screening T cells with antigens for specific populations |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20030203384A1 (en) * | 2002-03-08 | 2003-10-30 | Chafin David R. | Multiplex detection of biological materials in a sample |
US20050123937A1 (en) * | 2003-03-07 | 2005-06-09 | Thorp H. H. | Methods for the electrochemical detection of target compounds |
DE602004025371D1 (de) | 2003-11-10 | 2010-03-18 | Geneohm Sciences Inc | Nukleinsäurenachweisverfahren mit erhöhter empfindlichkeit |
US20060134657A1 (en) * | 2004-05-28 | 2006-06-22 | Nanogen, Inc. | Nanoscale electronic detection system and methods for their manufacture |
US20080003142A1 (en) * | 2006-05-11 | 2008-01-03 | Link Darren R | Microfluidic devices |
US9538657B2 (en) * | 2012-06-29 | 2017-01-03 | General Electric Company | Resonant sensor and an associated sensing method |
JP2013013375A (ja) * | 2011-07-05 | 2013-01-24 | Tosoh Corp | 電極上における核酸の増幅反応を伴う電気化学的検出方法 |
US9810659B2 (en) * | 2013-02-08 | 2017-11-07 | Board Of Trustees Of Michigan State Univeristy | Nanoparticle-serialized oligonucleotide methods, compositions, and articles |
-
2014
- 2014-10-24 US US15/031,849 patent/US10845333B2/en active Active
- 2014-10-24 JP JP2015543920A patent/JP6385356B2/ja not_active Expired - Fee Related
- 2014-10-24 CA CA2929042A patent/CA2929042A1/en not_active Abandoned
- 2014-10-24 EP EP14856487.5A patent/EP3064934B1/en active Active
- 2014-10-24 WO PCT/JP2014/078298 patent/WO2015060417A1/ja active Application Filing
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2016
- 2016-04-20 IL IL24522216A patent/IL245222B/en active IP Right Grant
Also Published As
Publication number | Publication date |
---|---|
EP3064934B1 (en) | 2020-06-03 |
US20170168012A1 (en) | 2017-06-15 |
JPWO2015060417A1 (ja) | 2017-03-09 |
US10845333B2 (en) | 2020-11-24 |
IL245222A0 (en) | 2016-06-30 |
WO2015060417A1 (ja) | 2015-04-30 |
IL245222B (en) | 2019-10-31 |
EP3064934A1 (en) | 2016-09-07 |
EP3064934A4 (en) | 2017-06-28 |
CA2929042A1 (en) | 2015-04-30 |
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