JP6378992B2 - Algae frozen processing method - Google Patents
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- JP6378992B2 JP6378992B2 JP2014197648A JP2014197648A JP6378992B2 JP 6378992 B2 JP6378992 B2 JP 6378992B2 JP 2014197648 A JP2014197648 A JP 2014197648A JP 2014197648 A JP2014197648 A JP 2014197648A JP 6378992 B2 JP6378992 B2 JP 6378992B2
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- 241000195493 Cryptophyta Species 0.000 title claims description 107
- 238000003672 processing method Methods 0.000 title claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 48
- 238000001816 cooling Methods 0.000 claims description 36
- 238000003860 storage Methods 0.000 claims description 31
- 238000000034 method Methods 0.000 claims description 27
- 238000007710 freezing Methods 0.000 claims description 26
- 230000008014 freezing Effects 0.000 claims description 26
- 238000005406 washing Methods 0.000 claims description 26
- 230000018044 dehydration Effects 0.000 claims description 17
- 238000006297 dehydration reaction Methods 0.000 claims description 17
- 230000002378 acidificating effect Effects 0.000 claims description 15
- 235000016425 Arthrospira platensis Nutrition 0.000 claims description 6
- 240000002900 Arthrospira platensis Species 0.000 claims description 6
- 241000195620 Euglena Species 0.000 claims description 6
- 229940082787 spirulina Drugs 0.000 claims description 6
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 4
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 claims description 4
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 4
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 4
- 229910052801 chlorine Inorganic materials 0.000 claims description 4
- 239000000460 chlorine Substances 0.000 claims description 4
- 238000003306 harvesting Methods 0.000 claims description 3
- 230000001953 sensory effect Effects 0.000 description 11
- 210000002421 cell wall Anatomy 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 4
- 239000000047 product Substances 0.000 description 3
- 241001474374 Blennius Species 0.000 description 2
- 239000000306 component Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000005057 refrigeration Methods 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 239000003673 groundwater Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000003002 pH adjusting agent Substances 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
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- Edible Seaweed (AREA)
- Freezing, Cooling And Drying Of Foods (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
本発明は，藻類の冷凍加工方法に関する。 The present invention relates to a method for freezing alga.
特開平６−３１９４９３号公報には，海藻類を洗浄殺菌して脱水した後にｐＨ調整剤を用いて酸性度を調整した上で冷凍保存する生食用海藻類の長期保存方法が開示されている。 Japanese Patent Laid-Open No. 6-319493 discloses a method for long-term preservation of raw edible seaweeds which is frozen and stored after adjusting the acidity with a pH adjuster after washing and sterilizing seaweeds.
特許第２７９０６４２号公報には，生ユーグレナを凍結乾燥または冷凍処理させたユーグレナ処理物が開示されている。 Japanese Patent No. 2790642 discloses a processed Euglena product obtained by freeze-drying or freezing fresh Euglena.
特許第４３８５５６６号公報には，スピルリナ顆粒の製造方法が開示されている。この方法は，スピルリナの乾燥粉末を粘土状にしてさらに乾燥させた後に造粒する。 Japanese Patent No. 4,385,566 discloses a method for producing Spirulina granules. In this method, the dried powder of spirulina is made into a clay and dried, and then granulated.
上記のとおり，藻類を保存するために冷凍加工することは知られている。しかしながら，従来の凍結された藻類は，藻類独特の臭気が強まるという問題がある。 As mentioned above, it is known to freeze to preserve algae. However, the conventional frozen algae has a problem that the odor peculiar to algae increases.
さらに，藻類を凍結乾燥させた後，粉末にするために加熱したものは，栄養価が下がるという問題もある。 Furthermore, there is also a problem that the nutritional value is lowered when the algae are freeze-dried and then heated to form a powder.
そこで，本発明は，藻類独特の臭気を抑えることができる藻類の冷凍加工方法を提供することを目的とする。 Then, an object of this invention is to provide the frozen processing method of algae which can suppress the odor peculiar to algae.
また，本発明は，栄養価が低減しない，冷凍加工方法を提供することを目的とする。 Another object of the present invention is to provide a frozen processing method that does not reduce the nutritional value.
本発明は，藻類の冷凍加工方法に関する。藻類の例は，細胞壁のない藻類である。細胞壁のない藻類は，微細な藻類であることが好ましく，細胞壁の無い微細な藻類の例は，スピルリナ及びユーグレナである。 The present invention relates to a method for freezing alga. An example of algae is algae without cell walls. Algae without cell walls are preferably fine algae, and examples of fine algae without cell walls are Spirulina and Euglena.
本発明の方法は，洗浄工程，冷却工程，初期冷凍工程，保存温度到達工程及び冷凍保存工程を含む。洗浄工程は，藻類を収穫後に洗浄する工程である。
冷却工程は，洗浄工程で洗浄された藻類を冷却し，０℃まで冷却する工程である。
初期冷凍工程は，冷却工程の後に，藻類を０℃の状態から−５℃の状態へ１５分以内に冷却する工程である。
保存温度到達工程は，藻類を−５℃の状態から−４０℃の状態（保存温度の状態）へ３０分以内に冷却する工程である。−４０℃とは厳密な意味での−４０℃でなくても構わない。すなわち，例えば，−４０℃の状態は，−３９℃以上−４１℃以下でも構わない。
冷凍保存工程は，保存温度到達工程の後に保存温度にて１時間以上藻類を冷凍保存する工程である。保存温度にて冷凍保存する際に，温度が厳密に一致し続けることはありえないため，例えば，保存温度の上下５℃（好ましくは２℃）以下の変動があっても構わない。
The method of the present invention includes a washing step, a cooling step, an initial freezing step, a storage temperature reaching step, and a frozen storage step. The washing step is a step of washing algae after harvesting.
The cooling step is a step of cooling the algae washed in the washing step to 0 ° C.
The initial freezing step is a step of cooling the algae from the 0 ° C. state to the −5 ° C. state within 15 minutes after the cooling step.
The storage temperature reaching step is a step of cooling the algae from a state of −5 ° C. to a state of −40 ° C. (a state of storage temperature) within 30 minutes. −40 ° C. may not be −40 ° C. in the strict sense. That is, for example, the −40 ° C. state may be −39 ° C. or more and −41 ° C. or less.
The frozen storage step is a step of freezing and storing algae at the storage temperature for 1 hour or more after the storage temperature reaching step. When the frozen temperature is stored at the storage temperature, the temperatures cannot be kept exactly the same. For example, the storage temperature may fluctuate by 5 ° C. or lower (preferably 2 ° C.) or less.
本発明の好ましい例は，冷却工程が，脱水・冷却工程と，水分調整工程とを更に含むものである。
脱水・冷却工程は，洗浄工程後の藻類を水分量が７５％以上８８％以下となるように脱水するとともに藻類を３〜５℃に冷却する工程である。脱水と冷却は別々に行っても良いし，同時に又は並行して行っても良い。水分量が７５％以上８８％以下とは，洗浄後の藻類の水分量を１００％とした場合における脱水工程後の水分量を意味する。
水分調整工程は，藻類に水を加えて藻類の水分量を９３％以上９８％以下となるように調整する工程である。この水分量も，洗浄後の藻類の水分量を１００％とした場合における藻類に含まれる水分量を意味する。
水分調整工程の後は，藻類を適宜冷却して０℃まで冷却する。なお冷却は，水分調整工程と並行して行っても良い。
In a preferred example of the present invention, the cooling step further includes a dehydration / cooling step and a moisture adjustment step.
The dehydration / cooling step is a step of dehydrating the algae after the washing step so that the water content is 75% or more and 88% or less and cooling the algae to 3 to 5 ° C. Dehydration and cooling may be performed separately or simultaneously or in parallel. The water content of 75% or more and 88% or less means the water content after the dehydration step when the water content of the washed algae is 100%.
The moisture adjustment step is a step of adding water to the algae and adjusting the moisture content of the algae to be 93% or more and 98% or less. This amount of water also means the amount of water contained in the algae when the amount of water in the washed algae is 100%.
After the moisture adjustment step, the algae are appropriately cooled and cooled to 0 ° C. The cooling may be performed in parallel with the moisture adjustment step.
本発明の好ましい例は，洗浄工程は，微酸性電解水を用いて藻類を洗浄する工程を含むものである。そして，微酸性電解水は酸性度がｐＨ５〜６．５の微酸性電解水が好ましく，有効塩素濃度が１０ｐｐｍ以上３０ｐｐｍ以下のものが好ましい。本発明の好ましい例は，洗浄工程の後，冷却工程の前に藻類をトレハロース溶液に浸漬するものである。 In a preferred example of the present invention, the washing step includes a step of washing algae using slightly acidic electrolyzed water. The slightly acidic electrolyzed water is preferably slightly acidic electrolyzed water having an acidity of pH 5 to 6.5, and preferably has an effective chlorine concentration of 10 ppm to 30 ppm. A preferred example of the present invention is to immerse algae in a trehalose solution after the washing step and before the cooling step.
本発明の好ましい例は，藻類を生きたまま冷凍保存するものである。すなわち，冷凍した藻類を解凍し，培養を行うと，藻類が増殖するものが好ましい。 A preferred example of the present invention is to preserve algae alive and frozen. That is, it is preferable that the algae grow when the frozen algae are thawed and cultured.
本発明の藻類の冷凍加工方法は，藻類を急速に冷凍するため，氷が成長し，細胞を破壊する事態を効果的に防止でき，その結果，藻類を生きたまま冷凍することができる。 The method for freezing alga of the present invention rapidly freezes algae, so that it is possible to effectively prevent the situation where ice grows and destroys cells, and as a result, the algae can be frozen alive.
以下，図面を用いて本発明を実施するための形態について説明する。本発明は，以下に説明する形態に限定されるものではなく，以下の形態から当業者が自明な範囲で適宜修正したものも含む。 Hereinafter, embodiments for carrying out the present invention will be described with reference to the drawings. The present invention is not limited to the embodiments described below, but includes those appropriately modified by those skilled in the art from the following embodiments.
本発明は，藻類の冷凍加工方法に関する。藻類の例は，細胞壁のない藻類である。細胞壁のない藻類は，微細な藻類であることが好ましく，細胞壁の無い微細な藻類の例は，スピルリナ及びユーグレナである。 The present invention relates to a method for freezing alga. An example of algae is algae without cell walls. Algae without cell walls are preferably fine algae, and examples of fine algae without cell walls are Spirulina and Euglena.
本発明の冷凍加工方法は，洗浄工程，冷却工程，初期冷凍工程，保存温度到達工程及び冷凍保存工程を含む。洗浄後の各種工程は，急速冷蔵可能な冷蔵装置を用いて行っても良い。また，洗浄藻類を急速に冷却するため，冷却工程，初期冷凍工程，又は保存温度到達工程をある装置で行った後に，別の装置にて冷凍保存工程を行っても良い。 The frozen processing method of the present invention includes a washing process, a cooling process, an initial freezing process, a storage temperature reaching process, and a frozen storage process. Various processes after washing may be performed using a refrigeration apparatus capable of rapid refrigeration. Further, in order to rapidly cool the washed algae, after performing the cooling process, the initial freezing process, or the storage temperature reaching process with a certain apparatus, the frozen storage process may be performed with another apparatus.
洗浄工程は，収穫後の藻類を洗浄する工程である。藻類は，例えば水槽で培養される。そして，培養された藻類は，フィルタやスクリーンを用いて選別される。選別された藻類を，水（例えば，地下水）に浸漬させた後に，脱水を行う。脱水は，フィルタやスクリーンを用いてろ過すればよい。脱水により，例えば，藻類に含まれる水分量が７０％以上８５％以下に軽減される。この浸漬及び脱水を，例えば１〜１０回繰り返す。このようにして，藻類から不純物を除去する。 The washing process is a process of washing the algae after harvesting. Algae are cultured in a water tank, for example. Then, the cultured algae are selected using a filter or a screen. After the selected algae are immersed in water (for example, groundwater), dehydration is performed. Dehydration may be performed using a filter or screen. By dehydration, for example, the amount of water contained in algae is reduced to 70% or more and 85% or less. This soaking and dehydration is repeated, for example, 1 to 10 times. In this way, impurities are removed from the algae.
次に，藻類を，水又は微酸性電解水を用いて洗浄する。この微酸性電解水を用いた洗浄工程も，藻類を微酸性電解水に浸漬し，脱水を行う。この浸漬及び脱水を，例えば１〜１０回（又は３〜５回）繰り返す。後述する実施例により実証されたとおり，微酸性電解水を用い藻類独特の臭いを軽減できる。微酸性電解水は，例えば希塩酸を電気分解して得られる弱酸性の液体である。微酸性電解水の例は，酸性度がｐＨ５〜６．５のものであり，有効塩素濃度が１０ｐｐｍ以上３０ｐｐｍ以下のものである。 Next, the algae are washed with water or slightly acidic electrolyzed water. In the washing process using the slightly acidic electrolyzed water, the algae is immersed in the slightly acidic electrolyzed water to perform dehydration. This soaking and dehydration is repeated, for example, 1 to 10 times (or 3 to 5 times). As demonstrated by the examples to be described later, slightly acidic electrolyzed water can be used to reduce the odor peculiar to algae. The slightly acidic electrolyzed water is a weakly acidic liquid obtained by electrolyzing dilute hydrochloric acid, for example. An example of slightly acidic electrolyzed water has an acidity of pH 5 to 6.5 and an effective chlorine concentration of 10 ppm to 30 ppm.
洗浄工程において洗浄されて得られる洗浄藻類を，適宜包装に充填してもよい。包装は，内容物を密閉できるものであればよい。
冷却工程は，洗浄工程で洗浄された藻類を冷却し，０℃まで冷却する工程である。冷却工程は，脱水・冷却工程と，水分調整工程とを更に含むものが好ましい。
脱水・冷却工程は，洗浄工程後の藻類を水分量が７５％以上８８％以下となるように脱水するとともに藻類を３〜５℃に冷却する工程である。脱水と冷却は別々に行っても良いし，同時に又は並行して行っても良い。水分量が７５％以上８８％以下とは，洗浄後の藻類の水分量を１００％とした場合における脱水工程後の水分量を意味する。
水分調整工程は，藻類に水を加えて藻類の水分量を９３％以上９８％以下となるように調整する工程である。この水分量も，洗浄後の藻類の水分量を１００％とした場合における藻類に含まれる水分量を意味する。
水分調整工程の後は，藻類を適宜冷却して０℃まで冷却する。なお冷却は，水分調整工程と並行して行っても良い。
初期冷凍工程は，冷却工程の後に，藻類を０℃の状態から−５℃の状態へ１５分以内に冷却する工程である。
保存温度到達工程は，藻類を−５℃の状態から−４０℃の状態（保存温度の状態）へ３０分以内に冷却する工程である。−４０℃とは厳密な意味での−４０℃でなくても構わない。すなわち，例えば，−４０℃の状態は，−３９℃以上−４１℃以下でも構わない。
冷凍保存工程は，保存温度到達工程の後に保存温度にて１時間以上藻類を冷凍保存する工程である。保存温度にて冷凍保存する際に，温度が厳密に一致し続けることはありえないため，例えば，保存温度の上下５℃（好ましくは２℃）以下の変動があっても構わない。
Washed algae obtained by washing in the washing step may be appropriately filled in a package. Any packaging that can seal the contents is acceptable.
The cooling step is a step of cooling the algae washed in the washing step to 0 ° C. The cooling step preferably further includes a dehydration / cooling step and a moisture adjustment step.
The dehydration / cooling step is a step of dehydrating the algae after the washing step so that the water content is 75% or more and 88% or less and cooling the algae to 3 to 5 ° C. Dehydration and cooling may be performed separately or simultaneously or in parallel. The water content of 75% or more and 88% or less means the water content after the dehydration step when the water content of the washed algae is 100%.
The moisture adjustment step is a step of adding water to the algae and adjusting the moisture content of the algae to be 93% or more and 98% or less. This amount of water also means the amount of water contained in the algae when the amount of water in the washed algae is 100%.
After the moisture adjustment step, the algae are appropriately cooled and cooled to 0 ° C. The cooling may be performed in parallel with the moisture adjustment step.
The initial freezing step is a step of cooling the algae from the 0 ° C. state to the −5 ° C. state within 15 minutes after the cooling step.
The storage temperature reaching step is a step of cooling the algae from a state of −5 ° C. to a state of −40 ° C. (a state of storage temperature) within 30 minutes. −40 ° C. may not be −40 ° C. in the strict sense. That is, for example, the −40 ° C. state may be −39 ° C. or more and −41 ° C. or less.
The frozen storage step is a step of freezing and storing algae at the storage temperature for 1 hour or more after the storage temperature reaching step. When the frozen temperature is stored at the storage temperature, the temperatures cannot be kept exactly the same. For example, the storage temperature may fluctuate by 5 ° C. or lower (preferably 2 ° C.) or less.
上記の冷凍保存工程の後は，通常の冷蔵保存を行っても構わない。冷蔵保存における藻類の温度は冷凍状態が維持できれば構わないため，−４０℃以上０℃以下（例えば−２５℃以上―１０℃以下）でもよい。 After the above frozen storage step, normal refrigerated storage may be performed. The temperature of the algae in the refrigerated storage may be -40 ° C. or higher and 0 ° C. or lower (for example, −25 ° C. or higher and −10 ° C. or lower) as long as the frozen state can be maintained.
上記の方法は，藻類を急速に冷凍する結果，藻類の細胞が氷により破壊される事態を防止でき，その結果，解凍後に藻類が活動できる（培養すれば藻類が増える）。このため，上記の方法は，生きたまま藻類を冷凍できる冷凍加工処理であるといえる。藻類の細胞が破壊されると，細胞内の成分や液体が細胞外へ放出される。これは，藻類独特の臭気を強めることとなる。本発明は，上記のとおり，藻類の細胞が氷により破壊される事態を防止できるため，藻類独特の臭いが強まる事態を効果的に防止できる。 As a result of the rapid freezing of the algae, the above method can prevent the algae cells from being destroyed by ice, and as a result, the algae can be activated after thawing (the more algae are cultured). For this reason, it can be said that said method is a freezing process which can freeze algae alive. When algae cells are destroyed, intracellular components and fluids are released out of the cell. This will strengthen the algae-specific odor. As described above, the present invention can prevent a situation in which algae cells are destroyed by ice, and thus can effectively prevent a situation in which a smell unique to algae is strengthened.
以下，実施例に基づいて本発明を説明する。
［実施例１］
静岡県掛川市の弊社掛川バイオセンタ―にて培養したスピルリナ及びユーグレナを培養池から回収・収穫した。その後，培地成分及び雑菌を除去するため該藻体を同容量の無菌水で浸漬及び脱水を繰り返して洗浄した。
洗浄後の藻体の水分量が８０％になるように脱水し，その後３℃まで冷却した。冷却した藻体に，３℃に冷やした水を加えて水分量を９５％に調整した。水分量を調整した藻類を冷却し０℃とした。藻類を更に冷却し，０℃から−５℃を１０分で冷却した後，５℃から４０℃を２０分で冷却した。−４０℃にて２時間保管した後，−２０℃の保管庫へ移して３０日間保存した。その後後，保管庫から凍結した藻類を取り出して流水解凍し，官能試験を行った。
Hereinafter, the present invention will be described based on examples.
[Example 1]
Spirulina and Euglena cultured at our Kakegawa Biocenter in Kakegawa City, Shizuoka Prefecture were collected and harvested from the culture pond. Then, in order to remove a culture medium component and various bacteria, this alga body was repeatedly immersed and dehydrated and washed with the same volume of sterile water.
The alga bodies after washing were dehydrated so that the water content was 80%, and then cooled to 3 ° C. Water cooled to 3 ° C. was added to the cooled algal bodies to adjust the water content to 95%. The algae whose water content was adjusted were cooled to 0 ° C. The algae was further cooled and cooled from 0 ° C to -5 ° C in 10 minutes, and then cooled from 5 ° C to 40 ° C in 20 minutes. After storing at −40 ° C. for 2 hours, it was transferred to a −20 ° C. storage and stored for 30 days. After that, frozen algae were taken out from the storage, thawed with running water, and a sensory test was conducted.
［実施例２］
０℃から−５℃を２０分で冷却した以外は，実施例１と同様にして藻類を凍結乾燥及び解凍し，官能試験を行った。
[Example 2]
Algae was freeze-dried and thawed in the same manner as in Example 1 except that the temperature was cooled from 0 ° C to -5 ° C in 20 minutes, and a sensory test was performed.
［実施例３］
−５℃から−４０℃を３０分で冷却し，−４０℃で１時間保管した以外は，実施例１と同様にして藻類を凍結乾燥及び解凍し，官能試験を行った。
[Example 3]
Algae was freeze-dried and thawed in the same manner as in Example 1 except that it was cooled from −5 ° C. to −40 ° C. in 30 minutes and stored at −40 ° C. for 1 hour, and a sensory test was performed.
［実施例４］
０℃から−５℃を２０分で冷却し，−５℃から−４０℃を３０分で冷却，−４０℃で１時間保管した以外は，実施例１と同様にして藻類を凍結乾燥及び解凍し，官能試験を行った。
[Example 4]
The algae were freeze-dried and thawed in the same manner as in Example 1 except that 0 ° C to -5 ° C was cooled in 20 minutes, -5 ° C to -40 ° C was cooled in 30 minutes, and stored at -40 ° C for 1 hour. The sensory test was conducted.
［比較例１］
０℃から−５℃を２０分で冷却し，−５℃から−４０℃を３０分で冷却した以外は，実施例１と同様にして藻類を凍結乾燥及び解凍し，官能試験を行った。
[Comparative Example 1]
Algae was freeze-dried and thawed in the same manner as in Example 1 except that the temperature was cooled from 0 ° C to -5 ° C in 20 minutes and from -5 ° C to -40 ° C in 30 minutes.
［比較例２］
−５℃から−４０℃を４５分で冷却した以外は，実施例１と同様にして藻類を凍結乾燥及び解凍し，官能試験を行った。
[Comparative Example 2]
The algae were freeze-dried and thawed in the same manner as in Example 1 except that the temperature was decreased from −5 ° C. to −40 ° C. in 45 minutes, and a sensory test was performed.
［比較例３］
−４０℃で３０分保管した以外は，実施例１と同様にして藻類を凍結乾燥及び解凍し，官能試験を行った。
[Comparative Example 3]
Algae was freeze-dried and thawed in the same manner as in Example 1 except that it was stored at −40 ° C. for 30 minutes, and a sensory test was performed.
［実施例５］
脱水工程及び水分調整工程を行わなかった以外は，実施例１と同様にして藻類を凍結乾燥及び解凍し，官能試験を行った。
[Example 5]
Algae was freeze-dried and thawed in the same manner as in Example 1 except that the dehydration step and the moisture adjustment step were not performed, and a sensory test was performed.
［実施例６］
無菌水の替わりに有効塩素濃度が２０ｐｐｍ，ｐＨ６の微酸性電解水を用いた以外は実施例１と同様にして藻類を凍結乾燥及び解凍し，官能試験を行った。
[Example 6]
Algae was freeze-dried and thawed in the same manner as in Example 1 except that slightly acidic electrolyzed water having an effective chlorine concentration of 20 ppm and pH 6 was used instead of sterile water, and a sensory test was performed.
［実施例７］
洗浄後の藻類を１０００ガウス〜１万ガウスの磁場に５分間さらした以外は，実施例１と同様にして藻類を凍結乾燥及び解凍し，官能試験を行った。
[Example 7]
The algae were freeze-dried and thawed in the same manner as in Example 1 except that the washed algae were exposed to a magnetic field of 1000 to 10,000 gauss for 5 minutes, and a sensory test was performed.
［実施例８］
藻類１００ｇに対して，２０ｇ以上１００ｇ以下のトレハロース溶液に浸したのちに，凍結した以外は，実施例１と同様にして藻類を凍結乾燥及び解凍し，官能試験を行った。後述する結果のとおり，凍結前に藻類をトレハロースに浸すことできわめて良好な藻類の凍結品を得ることができた。
[Example 8]
Algae was freeze-dried and thawed in the same manner as in Example 1 except that it was frozen after being immersed in a trehalose solution of 20 g or more and 100 g or less with respect to 100 g of algae, and a sensory test was performed. As the results to be described later, a very good algae frozen product could be obtained by immersing the algae in trehalose before freezing.
実施例及び比較例で得られた藻類にサンプル番号を付し，サンプル番号を表記し，どのようにして得られた藻類かを記載せずに，２０人に対して，臭い，味及び外観について，官能試験を行った。その結果を表１に示す。 A sample number is attached to the algae obtained in Examples and Comparative Examples, the sample number is written, and the odor, taste and appearance are given to 20 people without describing the obtained algae. The sensory test was conducted. The results are shown in Table 1.
表１に示される通り，本発明の藻類の冷凍加工方法は，凍結前の処理や凍結処理をコントロールすることで極めて有効な藻類の冷凍加工品を得ることができる。 As shown in Table 1, the method for freezing algae according to the present invention can provide a highly effective algae frozen product by controlling the pre-freezing treatment and the freezing treatment.
本発明は食品産業やサプリメント等の産業において利用されうる。
The present invention can be used in industries such as the food industry and supplements.
Claims (5)
前記洗浄工程で洗浄された藻類を冷却し，０℃まで冷却する冷却工程と，
前記冷却工程の後に，前記藻類を０℃の状態から−５℃の状態へ１５分以内に冷却する初期冷凍工程と，
前記初期冷凍工程の後に，前記藻類を−５℃の状態から保存温度の状態へ３０分以内に冷却する保存温度到達工程であって，前記保存温度は−４１℃以上−３９℃以下の工程と，
前記保存温度到達工程の後に前記保存温度にて１時間以上前記藻類を冷凍保存する冷凍保存工程と，
を含み、
前記冷却工程は，
前記洗浄工程後の前記藻類を水分量が７５％以上８８％以下となるように脱水するとともに前記藻類を３〜５℃に冷却する脱水・冷却工程と，
前記脱水・冷却工程の後に，前記藻類に水を加えて前記藻類の水分量を９３％以上９８％以下となるように調整する水分調整工程と，
を含む，
藻類の冷凍加工方法。 A washing step for washing the algae after harvesting , wherein the algae is Spirulina or Euglena ;
Cooling the algae washed in the washing step, and cooling to 0 ° C .;
An initial freezing step for cooling the algae from the 0 ° C. state to the −5 ° C. state within 15 minutes after the cooling step;
A storage temperature reaching step for cooling the algae from a state of −5 ° C. to a storage temperature state within 30 minutes after the initial freezing step, wherein the storage temperature is −41 ° C. or higher and −39 ° C. or lower; ,
A frozen storage step of freezing and storing the algae for 1 hour or more at the storage temperature after the storage temperature reaching step;
Only including,
The cooling step includes
A dehydration / cooling step of dehydrating the algae after the washing step so that the water content is 75% or more and 88% or less and cooling the algae to 3 to 5 ° C .;
After the dehydration and cooling step, water adjustment step of adding water to the algae to adjust the water content of the algae to 93% or more and 98% or less,
including,
Freezing processing method of algae.
前記洗浄工程は，微酸性電解水を用いて前記藻類を洗浄する工程を含む，方法。 A method for freezing algal as claimed in claim 1,
The washing step includes a step of washing the algae using slightly acidic electrolyzed water.
前記微酸性電解水は酸性度がｐＨ５〜６．５の微酸性電解水である，方法。 A method for freezing algae according to claim 2 ,
The method, wherein the slightly acidic electrolyzed water is slightly acidic electrolyzed water having an acidity of pH 5 to 6.5.
前記微酸性電解水は，有効塩素濃度が１０ｐｐｍ以上３０ｐｐｍ以下である，方法。 A method for freezing algae according to claim 2 ,
The method in which the slightly acidic electrolyzed water has an effective chlorine concentration of 10 ppm to 30 ppm.
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