JP6358809B2 - Lifetime prolonging agent, food and beverage composition for prolonging survival time - Google Patents

Description

TECHNICAL FIELD The present invention relates to a life-longening agent and a food / beverage product composition for life-longening.

  Aging is considered to progress due to various causes, for example, it is considered to progress when tissue cells are oxidized.

  Conventionally, as an anti-aging agent, for example, an anti-aging agent having anti-oxidation performance, which is considered to exhibit an anti-aging action by this anti-oxidation performance, is known.

As this type of anti-aging agent, for example, one containing an extract of white orchid flowers has been proposed (Patent Document 1).
Since the above-mentioned anti-aging agent has antioxidant performance, it is considered that this antioxidant performance can exert an anti-aging action.

JP 2013-177439 A

However, aging is thought to progress not only by oxidation of cellular tissues but also by chronic inflammation of cellular tissues.
Therefore, although the above-mentioned anti-aging agent has anti-oxidation performance, it has a problem that it is not necessarily excellent in anti-aging performance due to insufficient performance for suppressing chronic inflammation.

This invention makes it a subject to provide the lifetime extension agent which has anti-aging performance, and the food-drinks composition for lifetime extension in view of said problem .

Survival extenders according to the present invention, viewed contains an extract of flowers of Tilia cordata, it is characterized in that the extraction solvent of the extract is extraction solvent containing ethanol.
According to the life extension agent having such a configuration, it is considered that chronic inflammation of tissue cells, which is considered to be one of the important causes of aging, can be suppressed. Thereby, it is thought that the anti-aging agent which concerns on this invention can exhibit sufficient lifetime extension performance.

Increased survival for food or beverage composition according to the present invention, viewed contains an extract of flowers of Tilia cordata, it is characterized in that the extraction solvent of the extract is extraction solvent containing ethanol.

The survival period extending agent and the food / beverage product composition for extending survival period of the present invention have an effect of being excellent in survival period extension performance .

The graph which represented the survival rate of the mouse | mouth by the Kaplan-Meier survival curve (Kaplan-Meier survival curve). (A) The figure which shows the result (after feeding 10 months) evaluated about the aging degree score as an aging parameter. (B) The figure which shows the result (after feeding 12 months) evaluated about the aging degree score as an aging parameter. (A) The figure which shows the result evaluated about the occult blood as an aging parameter in a urine test. (B) The figure which shows the result evaluated about the white blood cell as an aging parameter in a urine test. (A) The figure which shows the result evaluated about the onset rate of the tumor as an aging parameter. (B) The figure which shows the result evaluated about the onset rate of the ulcer (skin ulcer) as an aging parameter. (A) The figure which shows the result of having evaluated (2-trial Y-maze test) about the brain function as an aging parameter. (B) The figure which shows the result of having evaluated the brain function as an aging parameter (a novel object recognition test).

  Below, one Embodiment of the anti-aging agent which concerns on this invention is described.

The anti-aging agent of this embodiment contains at least 1 sort (s) of the component contained in the flower part of Bodaiju, and the component contained in a button pin.
Specifically, the anti-aging agent of this embodiment is contained in the flower part of the body of Bodaiju by including, for example, a pulverized product (dried pulverized product) of the flower part of Bodaiju or an extract of the flower part of Bodaige. Ingredients included. Moreover, the anti-aging agent of this embodiment contains the component contained in a button pie, for example by including the button pulverized material (dry pulverized material) or the button pi extract.

The anti-aging agent of the present embodiment contains at least an extract of the flower part of Bodaiju obtained by extracting the flower part of Bodaiju with an extraction solvent in that the anti-aging agent becomes more excellent in anti-aging performance. preferable.
In general, 10 to 30% by mass of an extract of the flower part of Bodaiju is obtained in terms of dry matter with respect to the dry pulverized product of the flower part of Bodaige.

The body is a plant of the Tiliaceae family.
The extract of the flower part of the body is obtained by extracting the flower part of a plant belonging to the family Tiliaceae with an extraction solvent.

  Examples of the plant belonging to the family Tiliaceae include a tree plant (Tilia platyphyllos Scop.), A tree plant (Tilia. Cordata Mill.), A tree linden (Tilia. Europaea L.), and other related plants. Among these, Fuyubodaiju (Tilia. Cordata Mill.) Is preferable as a plant belonging to the family Tiliaceae in terms of superior anti-aging performance. That is, as the extract of the flower part of the body, the extract of the flower part of the flower body is preferable.

The extraction part of the lindenaceae plant is not particularly limited as long as it includes a flower part, and examples of the extracted part include a flower part with a bud on a petal.
In addition, a flower part is a part containing a petal and a bamboo leaf. The flower part may be either one of the petals or the bamboo leaves, or both.

As described above, the anti-aging agent of the present embodiment is, for example, a pulverized product obtained by drying and crushing the root bark of a Paeoniaceae plant, or the root bark of a Paeoniaceae plant. By including the button pi extract obtained by extracting with, the components contained in the button pi are included.
It is preferable that the anti-aging agent of this embodiment contains the button pi extract obtained by extracting the root bark of the plant of Paeoniaceae with an extraction solvent at the point which is more excellent in anti-aging performance.
The button pi extract is obtained by extracting the root bark of a Paeoniaceae plant with an extraction solvent.

The button pi is the root bark of a plant of the Paeoniaceae family.
Examples of the plant of Paeoniaceae include Paeonia suffruticosa (= Paeonia moutan) or other related plants. As a plant of Paeoniaceae, Paeonia suffruticosa (= Paeonia moutan) is preferable in that it is more excellent in anti-aging performance.

  Each of the above extracts can usually be in the form of an extract with the extraction solvent, a diluted solution thereof, a concentrated solution thereof, or a dried product from which the extraction solvent has been removed. Specifically, the extract can be in the form of, for example, a solution, a paste, a gel, or a powder.

Examples of the extraction solvent include water or aliphatic monohydric alcohols such as methanol, ethanol, and propanol; aliphatic polyhydric alcohols such as glycerin, propylene glycol, and 1,3-butylene glycol; ketones such as acetone; And organic solvents such as esters such as dioxane, acetonitrile and ethyl acetate; aromatics such as xylene, benzene and toluene; and alkyl halides such as chloroform.
These extraction solvents may be used alone or in combination of two or more. The mixing ratio of the extraction solvent to be mixed is not particularly limited and is appropriately adjusted.

  The extraction solvent is preferably an extraction solvent containing an aliphatic alcohol such as an aliphatic monohydric alcohol or an aliphatic polyhydric alcohol in that the anti-aging agent is more excellent in anti-aging performance. An extraction solvent containing a monohydric alcohol is more preferred, an extraction solvent containing ethanol is more preferred, and ethanol is most preferred.

The extraction method is not particularly limited, and a conventionally known general extraction method can be employed. In the extraction, the extracted part of the plant of Tiliaceae or the button pi can be used as it is or after drying as an extraction raw material.
Moreover, extraction solvent amount is 5-15 times amount (mass ratio) of an extraction raw material normally, extraction temperature is 20 degreeC-80 degreeC, and extraction time is 2 hours-3 days. After extraction, purification treatment such as filtration, deodorization, and decolorization can be appropriately performed as necessary.

It does not specifically limit as a density | concentration of the said extract contained in the said anti-aging agent, For example, 0.1-100 mass% in conversion of a dry matter is mentioned.
In addition, dry matter conversion is converting into the mass of the dry matter which is a residue remove | excluding the extraction solvent from the extract.

The mechanism of action by which the anti-aging agent of this embodiment suppresses chronic inflammation has not been completely elucidated at this stage, but is considered to be due to the following reasons.
As one of the causes of aging, it is considered that weak inflammation in cellular tissues becomes chronic. On the other hand, bone marrow mesenchymal stem cells (MSCs) are considered to accumulate at inflammatory sites in various cellular tissues and exert anti-inflammatory and tissue regeneration effects.
By incorporating the anti-aging agent of this embodiment into the body by oral ingestion, a component contained in the flower part of Bodaiju or a component contained in button pie enters the blood, and this component causes bone marrow mesenchymal system in the blood. It is thought that stem cells (MSC) are sent. Furthermore, bone marrow mesenchymal stem cells (MSC) sent into the blood accumulate in various tissues that have caused chronic inflammation. Then, it is considered that chronic inflammation is suppressed by bone marrow mesenchymal stem cells (MSC) accumulated in various tissues.
Examples of cell tissues that can be attracted and collected by bone marrow mesenchymal stem cells (MSC) include various tissues such as skin tissue, muscle tissue, cartilage tissue, and liver tissue.

Next, one embodiment of the anti-aging food and drink of the present invention will be described.
The anti-aging food / beverage product of this embodiment contains at least 1 sort (s) of the component contained in the flower part of Bodaiju, and the component contained in a button pin.

The anti-aging food / beverage products of this embodiment can be in forms (states) such as liquid, semi-solid, solid lump, and powder.
Specifically, the anti-aging food / beverage products of this embodiment are a drink (drink agent), a tablet, a capsule, or a jelly agent, for example. According to the anti-aging food and drink in such a form (aspect), a predetermined amount of the component contained in the flower part of Bodaiju or the component contained in the button pi is taken into the body by a single oral intake. be able to.
Specifically, when the anti-aging food / beverage product includes an extract of the flower part of bodaiju or an extract of button pi, according to the anti-aging food / beverage product containing this extract, it is calculated in terms of dry matter. A certain amount of bodaige flower part extract or button pi extract can be taken into the body.

  The anti-aging food and drink of this embodiment is preferably in the form (aspect) of a tablet or capsule. When the anti-aging food or drink is in the form (aspect) of a tablet or a capsule, for example, a predetermined amount of an extract of a flower part of bodaiju or a button pi extract can be easily put into the body after a predetermined period There is an advantage that it is easy to capture.

  When the aspect (shape) of the anti-aging food or drink of this embodiment is a beverage, a tablet, a capsule, a jelly, or the like, the extract of the flower part of the above-mentioned bodaiju is converted into a dry matter per agent. It is preferable to contain 25 mg-100 mg. Specifically, the anti-aging food / beverage product of the present embodiment is such that, for example, one to three agents per day are orally ingested by humans, so that the oral intake of the extract of the flower part of Bodaiju is dried. It is preferably formed so as to be 50 to 300 mg / day in terms of conversion.

  When the aspect (shape) is a beverage, a tablet, a capsule, a jelly, or the like, the anti-aging food or drink according to the present embodiment contains 100 mg to 400 mg of a dried pulverized product of the body portion of each body. Is preferred. Specifically, the anti-aging food / beverage product of the present embodiment has an oral intake of 100 parts of the dried pulverized flower of bodaiju, for example, by orally ingesting 1 to 10 agents per day. It is preferable to be formed so as to be ˜4000 mg / day.

  When the aspect (shape) of the anti-aging food and drink of this embodiment is a beverage, a tablet, a capsule, a jelly, or the like, the above button pie extract is 2.5 mg to 1 mg in terms of dry matter per agent. It is preferable to contain 200 mg. Specifically, the anti-aging food and drink of the present embodiment is such that, for example, by allowing a human to ingest 1 to 5 agents per day, the oral intake of the button pi extract is 2. It is preferable to form so that it may become 5-1000 mg / day.

  When the aspect (shape) is a beverage, a tablet, a capsule, a jelly, or the like, the anti-aging food or drink of the present embodiment preferably contains 10 mg to 800 mg of dried pulverized crumb per agent. Specifically, the anti-aging food / beverage product of the present embodiment, for example, allows a human to ingest 1 to 10 agents per day, so that the oral intake of dried pulverized crumb is 10 to 8000 mg / day. It is preferable to form so that it may become day.

  More specifically, the anti-aging food and drink products include, for example, soft drinks such as juice: sweets such as gum, candy, caramel, etc .: processed fishery products such as kamaboko and chikuwa: sausage, ham Livestock products such as: Noodles such as raw noodles, boiled noodles, buckwheat, sauces, soy sauce, sauce, sugar, honey, powdered candy, condiments such as syrups, dairy products such as cheese, butter, yogurt, alcoholic beverages, etc. Can be mentioned.

The anti-aging food and drink of this embodiment usually further contains water, additives and the like.
Examples of the additive include excipients, flavoring agents, colorants, preservatives, thickeners, stabilizers, gelling agents, antioxidants, and the like.
A general thing is employ | adopted as an additive.

  The excipient is not particularly limited, and for example, fine silicon dioxide particles, sucrose fatty acid ester, crystalline cellulose / sodium carboxymethylcellulose, calcium hydrogen phosphate, wheat starch, rice starch, corn starch, potato starch, dextrin, cyclohexane Examples include starches such as dextrin, crystalline celluloses, lactose, glucose, sugar, reduced maltose, starch syrup, fructooligosaccharides, emulsified oligosaccharides, and sugar alcohols such as sorbitol, erythritol, xylitol, lactitol, mannitol. These excipients are used alone or in combination of two or more.

For example, an anti-aging food or drink in the form of a tablet is formed by mixing at least an excipient such as starch or lactose and an extract of the flower part of Bodaiju and compressing.
In addition, for example, in anti-aging foods and drinks in the form of capsules, a powdery material in which an excipient such as starch or lactose and an extract of the flower part of bodaiju are mixed is highly soluble in water such as gelatin. It is formed by filling a molecular hollow capsule.
In addition, for example, anti-aging foods and drinks are formed into tablets and capsules by mixing dry pulverized crumbs and excipients in the same manner as described above.

  Examples of the flavoring agent include sweetening agents such as acesulfame K, erythritol, oligosaccharide, mannose, xylitol, and isomerized saccharide, or fruit juice extract. These taste agents are used alone or in combination of two or more.

  The anti-aging food / beverage products of this embodiment can be manufactured by a conventionally well-known general method.

  Subsequently, an embodiment of the anti-aging method of the present invention will be described.

The anti-aging method of this embodiment is to incorporate the above-mentioned anti-aging food and drink into the body by oral ingestion.
That is, the anti-aging method of the present embodiment has an oral intake step in which the above-mentioned anti-aging food and drink are orally ingested into the body, and this step suppresses the aging of animals that have been orally ingested.

The oral intake step is preferably performed once to three times / day, more preferably once or twice / day.
The oral intake step is preferably performed with a time interval of 2 to 8 hours.

In the oral intake step, the anti-aging food / beverage product is adjusted so that the extract of the flower part of Bodaiju is 0.225 g / kg or more in terms of dry matter with respect to the body weight kg of the animal taken orally at one time. It is preferable to ingest, and it is more preferable to ingest the anti-aging food / beverage product so as to be 0.55 g / kg or more.
In the oral ingestion step, the anti-aging food / beverage product is adjusted so that the extract of the flower part of Bodaiju is 2.0 g / kg or less in terms of dry matter with respect to the body weight kg of the animal to be ingested once. It is preferable to ingest, and it is more preferable to ingest the anti-aging food / beverage product so as to be 1.45 g / kg or less.

On the other hand, in the oral intake step, the anti-aging food / beverage product is adjusted so that the dry pulverized product of the flower part of Bodaiju is 0.9 g / kg or more with respect to the body weight kg of the animal taken orally at one time. It is preferable to ingest, and it is more preferable to ingest the anti-aging food / beverage product so as to be 2.2 g / kg or more.
In the oral intake step, the anti-aging food / beverage product is orally ingested so that the dry pulverized product of the body part of the body is 8.0 g / kg or less with respect to the body weight kg of the animal taken orally. Preferably, the anti-aging food / beverage product is orally ingested so as to be 5.8 g / kg or less.

Further, in the oral intake step, the anti-aging food or drink is orally ingested so that the button pie extract is 0.03 g / kg or more in terms of dry matter with respect to the body weight kg of the animal to be orally ingested. Preferably, the anti-aging food or drink is orally ingested so as to be 0.3 g / kg or more.
In the oral ingestion step, the anti-aging food or drink is orally ingested so that the button pi extract is 6.7 g / kg or less in terms of dry matter with respect to the body weight kg of the animal to be orally ingested. The anti-aging food and drink is more preferably taken orally so as to be 5.83 g / kg or less.

Further, in the oral intake step, the anti-aging food or drink may be orally ingested so that the dried pulverized crumb is 0.12 g / kg or more per kg of the body weight of the animal taken orally. Preferably, the anti-aging food or drink is orally ingested so as to be 1.2 g / kg or more.
In the oral intake step, it is preferable to orally ingest the anti-aging food / beverage product so that the dried pulverized pulverized product is 26.7 g / kg or less with respect to the body weight kg of the animal to be orally ingested, More preferably, the anti-aging food or drink is orally ingested so as to be 23.3 g / kg or less.

In the anti-aging method of the present embodiment, the above-mentioned anti-aging method is such that the extract of the flower part of Bodaiju per day is 0.225 g / kg or more in terms of dry matter with respect to the body weight kg of the animal taken orally. It is preferable to ingest foods and drinks, and it is more preferable to ingest the anti-aging foods and drinks so as to be 0.55 g / kg or more.
In addition, in the anti-aging method of the present embodiment, the above-mentioned anti-aging method is such that the extract of the flower part of Bodaiju per day is 6.0 g / kg or less in terms of dry matter with respect to the body weight kg of the animal taken orally. The aging food or drink is preferably taken orally, and the anti-aging food or drink is more preferably taken orally so as to be 4.35 g / kg or less.

In the anti-aging method of this embodiment, the anti-aging food / beverage product is administered so that the dry pulverized product of the flower part of the body is 0.9 g / kg or more per day with respect to the body weight kg of the animal taken orally. It is preferable to ingest, and it is more preferable to ingest the anti-aging food / beverage product so as to be 2.2 g / kg or more.
In addition, in the anti-aging method of the present embodiment, the anti-aging food or drink is added so that the dry pulverized product of the flower portion of the body is 24 g / kg or less per day with respect to the body weight kg of the animal taken orally. It is preferable to ingest, and it is more preferable to ingest the anti-aging food / beverage product so as to be 17.4 g / kg or less.

In the anti-aging method of this embodiment, the anti-aging food or drink is orally administered so that the button pie extract is 0.03 g / kg or more in terms of dry matter per day with respect to the body weight kg of the animal taken orally. It is preferable to ingest, and it is more preferable to ingest the anti-aging food / beverage product so as to be 0.3 g / kg or more.
Further, in the anti-aging method of the present embodiment, the anti-aging food or drink is orally administered so that the button pie extract is 20 g / kg or less in terms of dry matter per day with respect to the body weight kg of the animal taken orally. It is preferable to ingest, and it is more preferable to ingest the anti-aging food or drink product so that the amount is 17.5 g / kg or less.

In the anti-aging method of the present embodiment, the anti-aging food or drink is orally ingested so that the dried pulverized crumb is 0.12 g / kg or more per day with respect to the body weight kg of the animal to be orally ingested. The anti-aging food or drink is more preferably taken orally so as to be 1.2 g / kg or more.
In addition, in the anti-aging method of the present embodiment, the anti-aging food or drink is orally ingested so that the dried pulverized crumb is 80 g / kg or less per day with respect to the body weight kg of the animal to be ingested orally. It is more preferable that the anti-aging food or drink is orally ingested so as to be 70 g / kg or less.

  The anti-aging method of the present embodiment can be performed on humans or non-human animals. The anti-aging method of this embodiment is preferably performed non-therapeutically for human cosmetic purposes.

In the anti-aging method of this embodiment, a human adult is allowed to orally ingest the anti-aging food / beverage product so that the extract of the flower part of the body is 0.01 g or more in terms of dry matter per day. The anti-aging food or drink is more preferably taken orally so that the amount is 0.05 g or more.
Moreover, it is preferable to orally ingest the above-mentioned anti-aging food and drink so that the extract of the flower part of the body of a bougainge is 2.0 g or less in terms of dry matter per day for a human adult. More preferably, the anti-aging food or drink is orally ingested so that

On the other hand, in the anti-aging method of the present embodiment, it is preferable to orally ingest the anti-aging food / beverage product so that a human adult becomes 0.04 g or more of a dried body of Bodaige per day. More preferably, the anti-aging food or drink is orally ingested so as to be 2 g or more.
Moreover, it is preferable to orally ingest the anti-aging food and drink so that a human body adult may have 8.0 g or less of the dried body crushed per day, and the anti-aging so as to be 1.2 g or less. More preferably, the food or drink for food is taken orally.

In the anti-aging method of the present embodiment, it is preferable to orally ingest the anti-aging food or drink product so that a human puppy extract is 0.003 g or more in terms of dry matter per day for a human adult. More preferably, the anti-aging food or drink is orally ingested so as to be 0.025 g or more.
In addition, it is preferable to orally ingest the anti-aging food and drink product so that a human puppy extract is 7.5 g or less in terms of dry matter per day for a human adult, and 1.0 g or less. More preferably, the anti-aging food or drink is orally ingested.

In the anti-aging method of this embodiment, it is preferable to orally ingest the anti-aging food and drink so that a human adult has a dry pulverized crumb of 0.01 g or more per day. More preferably, the anti-aging food or drink is taken orally so as to achieve the above.
Moreover, it is preferable to orally ingest the anti-aging food and drink so that the dry pulverized product of button pi per day is 30 g or less for a human adult, and the anti-aging product is preferably 8.0 g or less. More preferably, the food or drink is taken orally.

  The anti-aging agent, anti-aging food and drink, and anti-aging method of the present embodiment are as illustrated above, but the present invention is applicable to the above-described anti-aging agent, anti-aging food and drink, and anti-aging method. It is not limited. Moreover, in this invention, the various form employ | adopted in a general anti-aging agent, the food-drinks for anti-aging, and the anti-aging method can be employ | adopted in the range which does not impair the effect of this invention.

  Next, although an Example is given and this invention is demonstrated in more detail, this invention is not limited to these.

Example 1
As an anti-aging agent, a commercially available bodaige flower part extract was prepared. The details of the extract of the flower part of Bodaiju are as follows.
“Extract from the flower part of Bodaiju” Maruzen Pharmaceutical Co., Ltd.
An extract obtained by extracting the flower part with the leaf part, extraction solvent: ethanol,
It is a solid extract, contains 50% by weight in terms of dry matter, and contains 50% by weight dextrin as an excipient.

(Example 2)
As an anti-aging agent, a commercially available crushed dried button pi was prepared. The details of the pulverized dried button pi are as follows.
“Dry pulverized pulverized product” manufactured by Nippon Powder Chemical Co., Ltd. In addition, from the pulverized dried pulverized button paste, an extract of 20 to 30% by mass in terms of dry matter is obtained by extraction treatment.

"Evaluation of mouse aging"
Mice were orally ingested (free intake) with the feed mixed with the anti-aging agent of Example 1 or 2, and the survival rate of the mice was measured. In addition, various aging parameters were evaluated while measuring the survival rate of mice.
Each condition in the evaluation test is as follows.

Evaluation mouse ICR retired (6-8 months old) (Japan SLC)
Feed (feed for long-term breeding):
CE-7 (feed consisting mainly of soybean meal) / anti-aging agent of Example 1 (1.5% by mass in terms of dry matter), or CE-7 / anti-aging agent of Example 2 (ground and dried) 10 mass% concentration). As a control feed, a feed containing none of the anti-aging agents of Example 1 and Example 2 was used.

[Aging parameters]
The degree of aging was evaluated by the following parameters (survival rate, aging score, urinalysis, tumor and ulcer incidence, brain function (behavior) evaluation).
<Survival>
Assessed by creating Kaplan-Meier survival curve Measurement N = 13 (breeding period: 60 weeks)
<Aging score>
Scores were obtained by scoring 11 items such as exploratory behavior, hair and skin roughness, degree of hair loss, corneal turbidity, and spinal flexion. In detail, the score was calculated | required by the following method generally used as one of the criteria of an anti-aging effect.
That is, the degree of aging that appears in the appearance of mice based on the grading score system (Toshio Takeda, development of aging-promoting model mice, intractable disease models and animal experiments, 180, (1984), Soft Science) As evaluation items for evaluating the above, behavior immediately after taking out the mouse from the gauge (reactivity), reaction when attempting to capture by hand from above (glossiness), glossiness, and roughness of hair and skin (coarness) ), Loss of hair, ulcer of the skin, periphalmic eruption, corneal opacity, corneal ulcer 11 items of turbidity (cataract) and spinal flexion are set, and the age of aging is determined for each evaluation item according to the following criteria (see Table 1) for the 6-week-old mouse group. The obtained 11 item scores were totaled to give a score. The degree of aging was measured every 3 weeks.
<Urinalysis>
Tests for glucose, protein, bilirubin, urobilinogen, pH, occult blood, ketone bodies, E nitrate, leukocytes, creatinine, etc. Measurement N = 13 (breeding period: 60 weeks)
FIG. 3 shows the evaluation results of occult blood and leukocytes, which are indicators of inflammation.
<Tumor and ulcer incidence>
The tumor (lymph tumor) was determined by observation of its appearance. Judgment was made when the tumor was found. Other visceral tumors were judged by necropsy at the time of death.
The ulcer (skin ulcer) was judged by observation of the appearance.
In the measurement, each group N = 13 and the breeding period was 60 weeks.
<Evaluation of brain function (behavior)>
1. 2-trial Y-maze test Specifically, in order to make the mouse recognize the spatial arrangement, first place several tall objects as marks on the outside of the arm of the Y-shaped maze, and fix this object. Prepared.
Next, the mouse was trained by placing the mouse in the device with one of the arms randomly closed and allowing the mouse to search freely for 10 minutes.
Then, one hour after the training, the blocked arms were opened and the mouse was searched for the three arms so that the three arms (start arm, familiar arm, novel arm) could freely move back and forth. A video of 5 minutes of action was recorded.
Finally, analysis was performed. That is, the time when all the limbs of the mouse entered the arm was regarded as entering, and the stay time of each arm was measured.
In the evaluation after 6 months, N = 7 in the control group, N = 10 in the group of Example 1, and N = 8 in the group of Example 2. In the evaluation after 12 months, N = 6 in the control group, N = 10 in the group of Example 1, and N = 8 in the group of Example 2.
2. Novel Object Recognition Test First, mice were acclimated for 3 days (minimum 2 days) by leaving them in their cage for 10 minutes.
Next, two objects (Lego) were affixed to the cage bottom with gummed tape or the like. The mice were placed in the center of the cage and trained. The video of the search was recorded for 10 minutes from directly above. Lego as a known object was collected and the surface was cleaned with ethanol.
Subsequently, after 1 hour from the training, a known object and a novel object (golf ball) at the time of training were attached to the bottom of the cage with gummed tape or the like, the mouse was placed in the cage, and the mouse was searched.
The video was recorded and the object search time was measured visually. Even if the mouse was on the object, it was not counted unless it was searching for the object, such as when the face of the mouse was facing up. Mouse passages were not counted.
Then, analysis was performed by the following formula using “search time in training”, “search time in test”, and “new object recognition rate (%)” as indices.
Novel object recognition rate (%) = (Time to search for novel object) / (Sum of time to search for both objects)
In the evaluation after 6 months, N = 9 in the control group, N = 10 in the group of Example 1, and N = 8 in the group of Example 2. In the evaluation after 12 months, N = 6 in the control group, N = 10 in the group of Example 1, and N = 8 in the group of Example 2.

A survival curve was generated by the Kaplan-Meier method. The result is shown in FIG.
As can be seen from FIG. 1, it can be said that the progress of aging in mice was suppressed by the anti-aging agents of Examples 1 and 2.

Table 2 and Table 3 and FIG. 2 show the aging score for the results of evaluation of aging parameters. Specifically, the results after 10 months of feeding are shown in Table 2 and FIG. 2 (a), and the results after 12 months of feeding are shown in Table 3 and FIG. 2 (b).
As can be seen from FIG. 2, it can be said that the progress of aging in mice was suppressed by the anti-aging agents of Examples 1 and 2.

The results of urinalysis, tumor and ulcer incidence, and brain function (behavior) evaluation are shown in FIGS.
Specifically, in FIG. 3, FIG. 3 (a) shows the results of occult blood in the urinalysis, and FIG. 3 (b) shows the results of leukocytes in the urinalysis.
In FIG. 4, FIG. 4 (a) shows the result of the incidence of tumor, and FIG. 4 (b) shows the result of the incidence of skin ulcer.
In FIG. 5, FIG. 5 (a) shows the result of the Y-maze test, and FIG. 5 (b) shows the result of the novel object recognition test.
As can be understood from FIGS. 3 to 5, it can be said that the progress of aging in mice was suppressed by the anti-aging agents of Examples 1 and 2.

  The anti-aging agent, food and drink, and anti-aging method of the present invention are used for, for example, delaying the progress of aging in animals such as humans.

Claims (2)

The Tilia cordata flower portion of the extract only contains,
The lifetime extension agent whose extraction solvent of the said extract is an extraction solvent containing ethanol . The Tilia cordata flower portion of the extract only contains,
The food / beverage product composition for extending a lifetime , wherein the extraction solvent of the extract is an extraction solvent containing ethanol .