JP2008237117A - Anti-fatigue food raw material and anti-fatigue food - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide anti-fatigue food raw material effective in recovery from fatigue; and to provide anti-fatigue food containing the food raw material. <P>SOLUTION: The anti-fatigue food raw material contains benzyl glucosinolate. A second version of the anti-fatigue food raw material comprises at least one kind of extract and/or concentrated extract selected from Lepidium meyenii-Walp, Cruciferae and Tropaeolum majus, and contains at least benzyl glucosinolate. A third version of the anti-fatigue food raw material comprises at least leaves, underground parts, seeds, sprouts or the like of one kind selected from Lepidium meyenii-Walp, Cruciferae and Tropaeolum majus, and contains at least benzyl glucosinolate. The anti-fatigue food contains the anti-fatigue food raw material. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  TECHNICAL FIELD The present invention relates to an anti-fatigue food material and an anti-fatigue food containing the food material, and more particularly to an anti-fatigue food material containing at least benzylglucosinolate and an anti-fatigue food containing the food material. .

  Currently, “fatigue” is a major social problem, and the word “Karoshi” is an unfamous international word for Japan. This refers to sudden death due to overcrowding for a long time, and is greatly highlighted as a social problem. Despite the fact that the problem of fatigue is socially and economically important, the countermeasures are still behind.

  Regarding anti-fatigue foods, for example, Patent Document 1 uses a food and drink containing an extract of roasted coffee beans and having an action of relieving mental stress by ingestion, for relieving mental stress. The effect is disclosed. Patent Document 2 discloses an anti-fatigue drink agent containing proanthocyanidins, grape seeds, skin extract or red wine extract.

  As these patent documents show, research on fatigue is gradually progressing. However, fatigue is still widespread in the modern society, and the present situation is that creation of anti-fatigue foods effective for fatigue recovery is desired.

Maca (Lepidium. Meyenii Walp.) Is a crop belonging to the Brassicaceae family (also known as Cruciferae) in the Andes. The Lepidium genus consists of approximately 175 species (Mummenhoff et al. 1992) and is widely distributed on all continents in the world except Antarctica. This maca is used locally as a folklore medicine and is known to be effective for anemia, infertility, or reduced energy. For example, Patent Document 3 discloses a functional food containing maca that has an effect of increasing blood growth hormone and increasing endurance. Patent Document 4 discloses a testosterone increasing composition containing maca.
JP 2006-081513 A JP-A-11-318402 JP 2004-000171 A JP 2005-306754 A

  This invention is made | formed in view of the said problem, and makes it a subject to provide the anti-fatigue food material and anti-fatigue food containing a natural product origin component. Specifically, it is an object to provide an anti-fatigue food material containing benzyl glucosinolate contained in maca (Lepidium meyenii-Walp, Cruciferae) as an active ingredient, and an anti-fatigue food containing the food material.

  The present inventors have found that benzylglucosinolate contained in a natural product, maca (Lepidium meyenii-Walp, Cruciferae), exhibits an excellent anti-fatigue effect, leading to the present invention.

  The invention according to claim 1 relates to an anti-fatigue food material containing benzylglucosinolate represented by the following formula (Formula 1).

請求項２に係る発明は、マカ（Lepidium meyenii-Walp, Cruciferae）及び／又はナスタチウム（Tropaeolum majus）の、抽出物及び／又は濃縮抽出物からなり、少なくとも下式（化２）で示されるベンジルグルコシノレートを含む抗疲労食品素材に関する。

The invention according to claim 2 comprises an extract and / or concentrated extract of maca (Lepidium meyenii-Walp, Cruciferae) and / or Nastathium (Tropaeolum majus), and is at least a benzyl group represented by the following formula (Formula 2). The present invention relates to an anti-fatigue food material containing cosinolate.

請求項３に係る発明は、マカ（Lepidium meyenii-Walp, Cruciferae）及び／又はナスタチウム（Tropaeolum majus）の、葉、地下部、種子、芽の少なくとも１つからなり、少なくとも下式（化３）で示されるベンジルグルコシノレートを含む抗疲労食品素材に関する。

The invention according to claim 3 is composed of at least one of a leaf, an underground part, a seed, and a bud of maca (Lepidium meyenii-Walp, Cruciferae) and / or Nastachium (Tropaeolum majus), and has at least the following formula (Formula 3): It relates to an anti-fatigue food material containing the indicated benzyl glucosinolate.

請求項４に係る発明は、前記抽出物及び／又は濃縮抽出物が、水及び／又はエタノールの抽出溶媒を使用して得られる抽出物及び／又は濃縮抽出物であることを特徴とする請求項２記載の抗疲労食品素材に関する。
請求項５に係る発明は、請求項１乃至４いずれかに記載の抗疲労食品素材を含有してなる抗疲労食品に関する。
請求項６に係る発明は、前記抗疲労食品素材が、ベンジルグルコシノレート含量０．００００１〜１０重量％となるように含有してなることを特徴とする請求項５記載の抗疲労食品に関する。

The invention according to claim 4 is characterized in that the extract and / or concentrated extract is an extract and / or concentrated extract obtained by using an extraction solvent of water and / or ethanol. 2. The anti-fatigue food material according to 2.
The invention according to claim 5 relates to an anti-fatigue food comprising the anti-fatigue food material according to any one of claims 1 to 4.
The invention according to claim 6 relates to the anti-fatigue food according to claim 5, wherein the anti-fatigue food material is contained so as to have a benzylglucosinolate content of 0.00001 to 10% by weight.

  Since the anti-fatigue food material and anti-fatigue food of the present invention contain benzyl glucosinolate as an active ingredient, they have an excellent fatigue recovery effect. Specifically, it suppresses a decrease in brain hormone secretion in a test subject in which fatigue has accumulated.

  Another advantage of the anti-fatigue food material and anti-fatigue food of the present invention is that it can reduce stress due to its antioxidant effect. In addition, its antioxidant effect prevents various symptoms that can be caused by aging, skin wrinkle formation, etc. caused by active oxygen, or lipid peroxides that are produced by oxidation of unsaturated fatty acids by active oxygen in the body. Can do.

  The anti-fatigue food material of the present invention includes benzyl glucosinolate (scientific name: Benzyl glucosinolate) represented by the following formula (Formula 4).

  In another embodiment, the anti-fatigue food material of the present invention comprises an extract and / or concentrated extract of maca (Lepidium meyenii-Walp, Cruciferae) and / or Nastathium (Tropaeolum majus), and at least the above formula (Formula 4). Benzyl glucosinolate shown in FIG.

  Maca (Lepidium meyenii-Walp, Cruciferae) (hereinafter referred to as Maca) according to the present invention is a perennial herb and is a root vegetable grown in alpine areas of an altitude of about 4000 to 4500 m in the central Andes such as Peru. It is used locally as a folklore medicine and is known to be effective for nutritional tonicity, malnutrition, anemia, infertility, or energy loss. Maca contains ingredients such as proteins, amino acids, minerals, vitamins and essential fatty acids.

  Nastachium (Tropaeolum majus) (hereinafter referred to as Nastatium) is a perennial plant grown in flower beds and potted plants as an annual sowing of spring, and its genus is climbing and has a length of 1-2 m, and its leaves resemble lotus leaves. It is like a bowl. Its origin is the highlands of the Andes in Peru, Colombia and Brazil, and the English name “Masturtium” is Dutch Dutch (Nasturtium officinale). .

  When the anti-fatigue food material of the present invention comprises an extract or concentrated extract of maca and / or nastathium, it can be used with any part of maca and / or nastathium, such as leaves, underground parts, seeds, and buds. Fatigue food materials may be produced, but preferably, an extract or concentrated extract of a maca underground part or a nastatiium leaf part is used. The underground part includes the hypocotyl and the root. Prior to extraction, a processed product obtained by previously drying, chopping, or crushing the plant may be extracted, or the maca and / or nastathium may be used as is without being processed at all. You may get

The method for producing an extract or concentrated extract of maca and / or nastathium according to the present invention is preferably as follows.
Macada and / or nastatium dried products, shredded products, or processed products such as pulverized products, or unprocessed maca and / or nastatium are extracted by heating at low temperature. Examples of the extraction solvent used at this time include water, ethanol, or a mixed solution of water and ethanol, and a mixed solution of water and ethanol is preferable. More preferred is an aqueous ethanol solution in which water: ethanol is mixed at a weight ratio of 1: 6-8. In particular, when an extract of a mixed solution of maca and / or nastathium in ethanol and water is used, benzylglucosinolate is preferentially extracted, so that it is excellent in industrial convenience and the obtained anti-fatigue food material has a high resistance. There will be a fatigue effect.
Furthermore, after filtering and obtaining an extract, this extract is concentrated. The concentrate may be used in the liquid state and the concentrated extract may be used as the anti-fatigue food material of the present invention. Alternatively, an extract obtained by freeze-drying or pulverizing after concentration may be used as the anti-fatigue food material of the present invention.

  As another embodiment, the anti-fatigue food material of the present invention is composed of one or more parts such as maca and / or nastathium leaves, underground parts, seeds, buds, etc., and is represented by at least the above formula (Formula 4). Contains benzyl glucosinolate. The underground part includes the hypocotyl and the root.

  In the said embodiment, although maca may use any site | parts, such as a leaf, an underground part, a seed, and a bud, Preferably an underground part is used. Nastatin may be used in any part such as leaves, underground parts, seeds, buds, etc., but preferably leaves are used. In order to make the anti-fatigue food material of the present invention, maca and / or nastathium may be used as they are without being processed, or the anti-fatigue food material is manufactured with a dried, shredded, or pulverized processed product. Also good.

As another embodiment, the anti-fatigue food material of the present invention may be manufactured using an extract or concentrated extract of papaya (Carica.papaya L.) containing at least benzylglucosinolate. Any part of papaya leaves, basement, seeds, buds, etc. may be used, but preferably papaya seeds are used. The underground part includes the hypocotyl and the root.
As an extraction method, a processed product such as a dried product, a shredded product, or a pulverized product of this seed, or an unprocessed papaya is extracted by heating at a low temperature. Examples of the extraction solvent used at this time include water and / or ethanol. After filtration to obtain an extract, the extract is concentrated. The concentrate may remain in a liquid state, that is, the concentrated extract may be used as the anti-fatigue food material of the present invention. Alternatively, the concentrate may be freeze-dried or pulverized to obtain an extract, which may be used as the anti-fatigue food material of the present invention.

  As another embodiment, the anti-fatigue food material of the present invention may use the papaya as it is. As a site | part used, you may use any site | parts, such as a papaya leaf, the hypodermis part containing a hypocotyl, and a root, a seed, a bud. Papaya seeds are preferably used.

  In another embodiment, the food material of the present invention is manufactured using an extract or concentrated extract of Wasinga (Moringa Oleifera) or Moringa stenopeneta containing at least benzylglucosinolate represented by the above formula (Formula 4). May be. Wasabikin or Moringa stenopetala leaves, hypocotyls including hypocotyls and roots, seeds, buds, roots, etc. may be used, but preferably horseradish or Moringa stenopetala leaves and roots are used, for example, A concentrated extract and / or extract can be obtained by the following method. A processed product such as a dried product, a shredded product, a pulverized product, or an unprocessed papaya is extracted by heating at a low temperature. Examples of the extraction solvent used at this time include water and / or ethanol. Further, after filtering to obtain an extract, the extract is concentrated. The concentrate may remain in a liquid state, that is, the concentrated extract may be used as the anti-fatigue food material of the present invention. Alternatively, the concentrate may be freeze-dried or pulverized to obtain an extract, which may be used as the anti-fatigue food material of the present invention.

  As another embodiment, the anti-fatigue food material of the present invention may use wasabi or Moringa stenopetala as it is. As a site | part used, you may use any site | parts, such as a horseradish or Moringa stenopetala leaf, a hypodermis part containing a hypocotyl and a root, a seed, a bud, a root. Preferably the leaves and roots of horseradish or Moringa stenopetala are used.

The anti-fatigue food of the present invention contains the anti-fatigue food material.
The anti-fatigue food of the present invention contains the anti-fatigue food material so that the benzylglucosinolate content is 0.00001 to 10% by weight. More specifically, when the anti-fatigue food of the present invention is in the form of a tablet such as a health food, the anti-fatigue food material is contained so that the benzylglucosinolate content is 0.1 to 3% by weight. For example, when it is set as drinking water, such as a PET bottle drink, it is contained so that it may become 0.00001-0.0001 weight%. For example, in taste foods such as confectionery and processed foods, the anti-fatigue food material is contained so that the benzylglucosinolate content is 0.00001 to 10% by weight. For this reason, if the benzyl glucosinolate exceeds 10% by weight in such a preferred food, it is not preferable because it is inferior to the touch and taste. In addition, if it is less than 0.00001% by weight, the anti-fatigue and anti-oxidation effects cannot be expected.

  The anti-fatigue food of the present invention preferably contains vitamin C in addition to the anti-fatigue food material. By containing this vitamin C, the anti-fatigue food of the present invention has an effect of suppressing active oxygen produced from oxidative stress and synergistically exhibits an excellent anti-fatigue effect.

In the anti-fatigue food of the present invention, conventional additives can be used depending on the type of food.
Examples of additives include excipients, pH adjusting agents, cooling agents, suspending agents, antifoaming agents, thickening agents, solubilizing agents, disintegrating agents, binders, lubricants, coating agents, and coloring agents. Agents, flavoring agents, surfactants or plasticizers.

  Examples of the excipient include sugar alcohols such as D-sorbitol, D-mannitol and xylitol, sugars such as glucose, sucrose, lactose and fructose, crystalline cellulose, carmellose sodium, calcium hydrogen phosphate, wheat starch, rice Examples thereof include starch, corn starch, potato starch, dextrin, β-cyclodextrin, light anhydrous silicic acid, titanium oxide, or magnesium aluminate metasilicate.

  Examples of the pH adjuster include citric acid, malic acid, sodium hydrogen phosphate, and dipotassium phosphate. Examples of the refreshing agent include l-menthol or mint water. Examples of the suspending agent include kaolin, carmellose sodium, xanthan gum, methylcellulose, and tragacanth. Examples of the antifoaming agent include dimethylpolysiloxane or silicon antifoaming agent.

  Examples of the thickener include xanthan gum, tragacanth, methylcellulose, and dextrin. Examples of the solubilizer include ethanol, sucrose fatty acid ester, and macrogol. Examples of the disintegrant include low-substituted hydroxypropylcellulose, carboxymethylcellulose calcium, croscarmellose sodium, hydroxypropyl starch, and partially pregelatinized starch. Examples of the binder include methylcellulose, hydroxypropylcellulose, hydroxypropylmethylcellulose, polyvinylpyrrolidone, gelatin, gum arabic, ethylcellulose, polyvinyl alcohol, pullulan, pregelatinized starch, agar, tragacanth, sodium alginate or propylene glycol alginate. Can be mentioned.

  Examples of the lubricant include stearic acid, magnesium stearate, calcium stearate, polyoxyl stearate, cetanol, talc, hydrogenated oil, sucrose fatty acid ester, dimethylpolysiloxane, beeswax and beeswax.

  The anti-fatigue food of the present invention can be mixed with other physiologically active ingredients, minerals, vitamins, hormones, nutritional ingredients, flavors, and other additives as necessary. Any of these additives generally used in foods can be used.

  The anti-fatigue food of the present invention can be processed into a shape such as a capsule such as a hard capsule or a soft capsule, a tablet, a pill, or a granule. The anti-fatigue food of the present invention can be supplied as a health food, for example.

  As another embodiment, the anti-fatigue food material of the present invention may be added to food to produce an anti-fatigue food. Examples of the food include confectionery such as snacks, biscuits, cookies, and chocolate, and drinking water such as juice and tea.

In addition, it is preferable to ingest the anti-fatigue food of this invention so that the daily intake of benzylglucosinolate may be 1-5 mg. When the daily intake is less than 1 mg, a sufficient anti-fatigue effect cannot be expected, and even if the intake exceeds 5 mg, it is estimated that no further effect can be expected.
Next, the present invention will be described more specifically with reference to examples.

  About the benzyl glucosinolate contained in the anti-fatigue food material of this invention, the test regarding the anti-fatigue effect and the antioxidant effect was done using the mouse | mouth.

(Experimental method / use mouse and its breeding / testing method)
For the test, BALB / c mice, females, 8 weeks of age were used. (1) Control exercise load group (20 mice) (2) Control light / dark load group (19 mice), (3) Exercise with benzylglucosinolate Load group (20 animals), (4) benzylglucosinolate administration light / dark load group (19 animals). The BALB / c mice (1) to (4) were all preliminarily reared.

  Refer to FIG. 1 for the test method. In FIG. 1, benzylglucosinolate (Benzyl glucosinolate [Lot. 07300-102, 12) was administered to the benzylglucosinolate administration group ((3) and (4)) for 12 weeks between [administration start] and [administration end]. CHROMADEC INC]) was orally administered once a day at a rate of 0.03 mg / kg. For the control group ((1) and (2)), the same amount of distilled water was orally administered for the same period.

The loading starts 4 weeks after [start of administration]. In [Load start] in FIG. 1, an animal treadmill (product name: small running ball, manufacturer: MR-24 Marcan Co., Ltd.) is used for the exercise load group ((1) and (3)) for 5 days / I exercised 20 minutes a day a week. The light and dark load groups ((2) and (4)) were illuminated daily for 45 minutes with a 200 W incandescent lamp (product name: clip lamp, manufacturer: LC-200, Takagi) from a position 30 cm from the cage. .
In FIG. 1, at the end of load, the exercise load and the light / dark load were completed. It is 4 weeks from [Load start] to [Load end].

  Four weeks after [end of loading], administration (benzyl glucosinolate for benzyl glucosinolate administration group ((3) and (4)), distilled water for control group ((1) and (2))) finished. In addition, [end of loading] to [end of administration] is 4 weeks.

(Blood collection method)
At the start of loading, at the end of loading, and at the end of administration, blood group measurement, fundus blood collection, and heart blood collection were performed from each group, and serum was collected from the obtained blood and stored frozen. After completion of all blood collections, brain hormones were measured for each serum using antioxidant concentration and EIA kit.
(Weight measurement)
Mice were weighed every week from the start of administration to the end of administration.

(AAPH measurement method and result by luminol reaction)
Serum obtained from a group of mice ((1) to (4)) was diluted 100-fold with 0.1 M PBS (pH 7) and added with AAPH reagent as a sample. A sample was inserted into a luminescence reader, and a luminol reagent was injected at 37 ° C. for 10 minutes, and the amount of luminescence was measured. The smaller the amount of luminescence, the higher the radical scavenging ability. That is, it can be said that the smaller the amount of luminescence, the higher the antioxidant concentration. The results are shown in FIG.

  As shown in FIG. 2, a decrease in AAPH luminescence was observed in the benzylglucosinolate administration group compared to the control group. That is, the antioxidant effect was shown in the benzyl glucosinolate administration group. Therefore, since the benzyl glucosinolate according to the present invention exhibits an antioxidant effect, it can be suitably used as an antioxidant food material or an antioxidant food.

(Mouse weight measurement results)
Please refer to FIG. As shown in FIG. 3, the benzylglucosinolate-administered group showed less weight loss during loading compared to the control group. That is, weight loss due to fatigue load was suppressed by administration of benzylglucosinolate.

(Test results on immune enhancement effect)
FIGS. 4 to 6 show the numbers of leukocytes (FIG. 4), lymphocytes (FIG. 5), and granulocytes (FIG. 6) in the serum of (1) to (4) mouse groups. As shown in these figures, suppression of decrease in the number of leukocytes and lymphocytes and granulocytes was observed in the light and dark loaded mice. In the exercised mice, the decrease in the number of leukocytes and lymphocytes and the increase in the number of granulocytes were observed. From this, it can be seen that administration of benzylglucosinolate improved the decrease in immune function due to exercise and light and dark load.

(Measurement result of hormones in the brain using EIA kit)
FIG. 7 shows the results of measuring the serotonin concentration in the serum of (1) to (4) mouse groups. As shown in FIG. 7, in the benzylglucosinolate administration group, suppression of the decrease in serotonin concentration was observed at the end of the load. Therefore, it is considered that depression was reduced by suppressing the decrease in serotonin concentration by administration of benzyl glucosinolate.
FIG. 8 shows the results of measuring the dopamine concentration in the serum of (1) to (4) mouse groups. In the benzylglucosinolate administration group, an increase in dopamine concentration was observed at the end of loading. Therefore, it is thought that the feeling of fatigue was reduced by the increase in dopamine concentration by administration of benzylglucosinolate.

  From the above test results, it was confirmed that benzylglucosinolate relieves stress by an antioxidant effect and suppresses a decrease in brain hormone secretion in fatigue mice.

  Furthermore, about the benzyl glucosinolate contained in the anti-fatigue food material of this invention, the test regarding the endurance improvement effect and the anti-fatigue effect was done using the mouse | mouth.

(Influence on swimming exercise by administration of benzylglucosinolate)
Std: ddY mice, 4 weeks old males were preliminarily raised for 7 days, then (A) control, (B) maca extract 30 mg / kg, (C) benzylglucosinolate 0.03 mg / kg, (D) benzylglu Administration was carried out in groups of 0.015 mg / kg of cosinolate and 0.0075 mg / kg of (E) benzylglucosinolate. (Second time n = 10)
In addition, (B) Maca extract is obtained by extracting the underground part of maca with hydrous ethanol and pulverizing it by freeze-drying.

(Forced swimming experiment)
A weight of 10% of the body weight was applied to the tail of the mouse, the time until the mouse completely submerges the head under the water for 5 seconds was measured, and the swimming duration was determined as the endurance time. Swimming exercise was carried out every week. The results are shown in FIG. In FIG. 9, the horizontal axis represents time (weeks) and the vertical axis represents time (minutes).

(Forced swimming experiment for a certain time)
The mouse is loaded with a 5% weight and allowed to perform a 15 minute swimming exercise before, during the swimming exercise (5, 10, 15 minutes) or after the swimming exercise (10, 30, 60 minutes). Blood was collected from the tail, and blood glucose (FIG. 10), blood lactic acid (FIG. 11), and free fatty acid (FIG. 12) were measured. The horizontal axis of the graphs of FIGS. 12 and 13 is time (minutes). Further, FIG. 13 shows changes in the amount of liver glycogen before and after swimming exercise, and FIG. 14 shows changes in the weight of adipose tissue after administration for 5 weeks.

As shown in FIGS. 9 to 14, in the mice administered with the maca extract or benzylglucosinolate according to the present invention, an increase in the swimming time of the mice was observed, and an increase in blood lactic acid due to swimming exercise was suppressed. During exercise, an increase in free fatty acids in the blood was observed. Furthermore, benzylglucosinolate reduced the fat tissue weight of mice and suppressed the decrease of tissue glycogen during exercise. From the above, the food material of the present invention not only provides anti-fatigue and antioxidant effects, but also promotes lipid metabolism rather than glucose metabolism during exercise, and the food material of the present invention is used as an energy source during exercise It turns out that it can be done.
That is, since the food material of the present invention has endurance improvement and anti-fatigue effects, it can be suitably used as an anti-fatigue food material.

(Test on the effect of maca extract on blood hemoglobin concentration)
Four-week-old mice (n = 10) were used and divided into three groups, a control group and two administration groups (Maca extract 6 mg / kg, 30 mg / kg), and oral administration was performed for 4 weeks. This maca extract is obtained by extracting the underground part of maca with hydrous ethanol and pulverizing it after freeze-drying. Each group was measured for blood hemoglobin concentration after 4 weeks of administration. The results are shown in FIG.

  As shown in FIG. 15, an increase in blood hemoglobin concentration was observed in mice administered with maca extract. As a result, it was found that the maca extract according to the present invention can serve as an energy source during exercise since the oxygen supply amount to the tissue increases due to an increase in blood hemoglobin concentration.

It is a figure for demonstrating the test method (breeding and test method of a mouse) in the Example of this invention. (1) Control exercise load group, (2) Control light / dark load group, (3) Benzylglucosinolate administration exercise load group, (4) Benzylglucosinolate administration light / dark load group, “administration start” to “administration end” 1 shows body weight measurement results for each week (mean ± standard error (t-test (n = 8)) / * P <0.05, ** P <0.01 vs control group). (1) Control exercise load group, (2) Control light / dark load group, (3) Benzylglucosinolate-administered exercise load group, (4) Benzylglucosinolate-administered light / dark load group, APPH fermentation measurement results (mean ± standard error) (T-test (n = 8)) / * P <0.05, ** P <0.01 vs control group). (1) Control exercise load group, (2) Control light / dark load group, (3) Benzylglucosinolate administration exercise load group, (4) Benzylglucosinolate administration light / dark load group measurement results (mean ± standard) Error (t-test (n = 8))). (1) Control exercise load group, (2) Control light / dark load group, (3) Benzylglucosinolate administration exercise load group, (4) Benzylglucosinolate administration light / dark load group measurement results (mean ± Standard error (t-test (n = 8)) / ** P <0.01 vs control group) is shown. (1) Control exercise load group, (2) Control light / dark load group, (3) Benzylglucosinolate administration exercise load group, (4) Benzylglucosinolate administration light / dark load group measurement results (mean ± Standard error (t-test (n = 8)) / ** P <0.01 vs control group) is shown. (1) Control exercise load group, (2) Control light / dark load group, (3) Benzylglucosinolate administration exercise load group, (4) Benzylglucosinolate administration light / dark load measurement results (mean ± standard) Error). (1) Control exercise load group, (2) Control light / dark load group, (3) Benzylglucosinolate administration exercise load group, (4) Benzylglucosinolate administration light / dark load group measurement results (mean ± standard) Error). (A) Control, (B) Maca extract 30 mg / kg group, (C) Benzylglucosinolate 0.03 mg / kg group, (D) Benzylglucosinolate 0.015 mg / kg group, (E) Benzylglucosino It is a forced swimming experiment result in a rate 0.0075 mg / kg group. (Average ± standard error is shown. Control group *: p <0.05, **: p <0.01, ***: p <0.005) (A) Control, (B) Maca extract 30 mg / kg group, (C) Benzylglucosinolate 0.03 mg / kg group, (D) Benzylglucosinolate 0.015 mg / kg group, (E) Benzylglucosino This is a change in blood glucose level due to a 15-minute swimming exercise load in the rate 0.0075 mg / kg group. (Average value ± standard error value is shown. Control group *: p <0.05, **: p <0.01, ***: p <0.005) (A) Control, (B) Maca extract 30 mg / kg group, (C) Benzylglucosinolate 0.03 mg / kg group, (D) Benzylglucosinolate 0.015 mg / kg group, (E) Benzyl The change of the amount of lactic acid in blood by the swimming exercise load for 15 minutes in the glucosinolate 0.0075mg / kg group is shown. (Average value ± standard error value is shown. Control group *: p <0.05, **: p <0.01, ***: p <0.005) (A) Control, (B) Maca extract 30 mg / kg group, (C) Benzylglucosinolate 0.03 mg / kg group, (D) Benzylglucosinolate 0.015 mg / kg group, (E) Benzyl group The change of the amount of free fatty acids in the blood by the swimming exercise load for 15 minutes in the cosinolate 0.0075 mg / kg group is shown. (Average values ± standard error values are shown. For control group *: p <0.05, **: p <0.01, ***: p <0.005) (A) Control, (B) Maca extract 30 mg / kg group, (C) Benzylglucosinolate 0.03 mg / kg group, (D) Benzylglucosinolate 0.015 mg / kg group, (E) Benzyl group The change in the amount of liver glycogen before and after swimming exercise in the cosinolate 0.0075 mg / kg group is shown. (Mean ± standard error) ***: p <0.01 before swimming exercise, #: p <0.05, ###: p <0.005 (after swimming exercise) for the control group, in the figure Black graph before swimming exercise, and white graph after swimming exercise.) (A) Control, (B) Maca extract 30 mg / kg group, (C) Benzylglucosinolate 0.03 mg / kg group, (D) Benzylglucosinolate 0.015 mg / kg group, (E) Benzyl group The change of the fat tissue weight in a cosinolate 0.0075mg / kg group is shown. (Mean value ± standard error value. Control group *: p <0.05) It is a test result regarding the influence of the maca extract on blood hemoglobin concentration.

Claims (6)

An anti-fatigue food material containing benzylglucosinolate represented by the following formula (Formula 1).

An anti-fatigue food material comprising an extract and / or concentrated extract of maca (Lepidium meyenii-Walp, Cruciferae) and / or Nastachium (Tropaeolum majus) and containing at least the benzylglucosinolate represented by the following formula (Chemical Formula 2) .

Maca (Lepidium meyenii-Walp, Cruciferae) and / or Nastachium (Tropaeolum majus) consisting of at least one of leaves, basement, seeds, and buds, and containing at least benzylglucosinolate represented by the following formula (Formula 3) Anti-fatigue food material.

  The anti-fatigue food material according to claim 2, wherein the extract and / or the concentrated extract is an extract and / or a concentrated extract obtained by using an extraction solvent of water and / or ethanol.   5. An anti-fatigue food comprising the anti-fatigue food material according to claim 1.   6. The anti-fatigue food material according to claim 5, wherein the anti-fatigue food material is contained so as to have a benzylglucosinolate content of 0.00001 to 10% by weight.

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