JP6238238B2 - Water purification treatment apparatus and water purification treatment method - Google Patents

Description

  The present invention relates to a water purification treatment apparatus and a water purification treatment method.

  With the progress of urbanization and industrial development in recent years, water pollution due to domestic wastewater and industrial wastewater has become a problem in waterstops (closed waters) such as lakes, ponds, dams, reservoirs, and reservoirs. In particular, the south lake of Lake Biwa has a low water discharge compared to the north lake, and convection is less likely to occur due to the effects of global warming. Has been.

  As an apparatus for purifying the water quality in such a water stop area, for example, an aeration stirrer disclosed in Patent Document 1 is known.

JP 2007-50353 A

  The water purification process using the aeration stirrer mentioned above, etc., improves the dissolved oxygen concentration by sending a large amount of air into the water stop area and promotes the growth of aerobic microorganisms, thus destroying the environment in the water stop area. There is a problem that there is a possibility that the energy cost associated with the operation of the apparatus is high. Furthermore, after the device is removed, the dissolved oxygen concentration decreases and the water quality deteriorates immediately. Therefore, it is necessary to periodically perform water purification treatment.

  The present invention has been made in view of the above-described problems of the prior art, and its main purpose is to continuously and inexpensively and based on the natural circulation system without destroying the environment in the water area to be treated. To provide a water purification treatment apparatus and a water purification treatment method capable of continuous treatment.

  Usually, in a still water area with good water quality, gentle convection occurs, and the pollutants are decomposed by microorganisms living in the water area. The inventors of the present invention have made extensive studies to achieve the above-described object with reference to the environment in such a good water stop area. As a result, by introducing the water in the target water area into the aerobic condition system and the anaerobic condition system, the microorganisms living in the target water area are adsorbed in each system, and the microorganisms decompose the pollutants such as organic matter. It was found that the water quality of the target water area can be purified. Furthermore, the present inventors have found that gentle convection can be generated in the water area to be treated by introducing and discharging water from the water area to be treated to each system. Based on this knowledge, the present invention has been completed by further research.

That is, the present invention typically provides the water purification treatment apparatus and the water purification treatment method described in the following section.
Item 1.
An aerobic microorganism treatment unit having a microorganism-adsorbing porous carrier;
A first circulation path for introducing and discharging water to be treated into the aerobic microorganism treatment section;
A first pumping means connected to the first circulation path and pumping the water to be treated to the aerobic microorganism treatment unit;
An anaerobic microorganism treatment section having a microorganism-adsorbing porous carrier;
A second circulation path for introducing and discharging water to be treated into the anaerobic microorganism treatment unit;
A water purification treatment apparatus comprising: a second pumping unit connected to the second circulation path and pumping the water to be treated to the anaerobic microorganism treatment unit.
Item 2.
The flow rate per unit time of the water to be treated that is pumped by the first pumping means is larger than the flow rate per unit time of the water to be treated that is pumped by the second pumping means. Item 2. A water purification treatment apparatus according to Item 1.
Item 3.
Item 3. The water purification treatment apparatus according to Item 1 or 2, further comprising air introduction means for introducing air into the aerobic microorganism treatment unit.
Item 4.
The water purification treatment apparatus according to any one of Items 1 to 3, further comprising: a pumping control unit that stops pumping of the water to be processed to the anaerobic microorganism processing unit for a predetermined period.
Item 5.
A third circulation path for introducing and discharging the water to be treated;
A third pumping means connected to the third circulation path and pumping the water to be treated;
Any one of the items 1 to 4 above, wherein the introduction port of the first circulation path and the second circulation path, or both the introduction port and the discharge port are connected to the third circulation path. The water purification treatment apparatus according to item.
Item 6.
A third circulation path for introducing and discharging the water to be treated;
A third pumping means connected to the third circulation path and pumping the water to be treated;
A water storage section for storing the water to be treated introduced from the third circulation path;
With
The third circulation path and the water storage section are connected by a water flow path, and the introduction port of the first circulation path and the second circulation path, or both the introduction port and the discharge port are connected to the water storage section. Item 5. The water purification treatment device according to any one of Items 1 to 4, wherein
Item 7.
The above items 1 to 6, wherein the microorganism-adsorbing porous carrier contains at least one resin selected from the group consisting of polyvinyl alcohol resins, polyurethane resins, polyolefin resins, polyethylene glycol resins, and polyether resins. The water treatment apparatus as described in any one of.
Item 8.
(A) introducing water to be treated into an aerobic microorganism treatment unit having a microorganism-adsorbing porous carrier and an anaerobic microorganism treatment unit having a microorganism-adsorbing porous carrier,
(B) a step of advancing decomposition treatment with aerobic microorganisms in the aerobic microorganism treatment unit and decomposition treatment with anaerobic microorganisms in the anaerobic microorganism treatment unit with respect to the water to be treated introduced in the step; And (c) discharging the water to be treated from the aerobic microorganism treatment unit and the anaerobic microorganism treatment unit,
The flow rate per unit time of the water to be treated introduced into the aerobic microorganism treatment unit is larger than the flow rate per unit time of the water to be treated introduced into the anaerobic treatment unit,
Water purification treatment method.
Item 9.
9. The water purification treatment method according to item 8, wherein convection is generated in the water area to be treated by the steps (a) and (c).
Item 10.
Including introducing the treatment target water from the treatment target water area into the circulation path and causing convection in the treatment target water area by discharging the treatment target water through the circulation path;
In the item (8), the water to be treated is introduced from the circulation path in the step (a), and the water to be treated is discharged to the circulation path or the water area to be treated in the step (c). The water purification treatment method as described.
Item 11.
Including introducing the treatment target water from the treatment target water area into the circulation path and causing convection in the treatment target water area by discharging the treatment target water through the circulation path;
In the step (a), the water to be treated is introduced from the circulation path, and in the step (c), the water to be treated is discharged to the water storage part, and then the water to be treated is discharged from the water storage part to the water area to be treated. The water purification treatment method according to Item 8, wherein the water purification treatment method is performed.
Item 12.
The water purification treatment method according to any one of Items 8 to 11, wherein air is introduced into the aerobic microorganism treatment unit together with the water to be treated.
Item 13.
13. The water purification treatment method according to any one of Items 8 to 12, wherein the introduction of water to be treated into the anaerobic microorganism treatment unit is stopped for a predetermined period.
Item 14.
Item 8-13, wherein the microorganism-adsorbing porous carrier contains at least one resin selected from the group consisting of polyvinyl alcohol resins, polyurethane resins, polyolefin resins, polyethylene glycol resins, and polyether resins. The water purification processing method as described in any one of these.

  The present invention is a biomimicry type water purification treatment apparatus that follows the natural environment. According to the present invention, it is possible to decompose a pollutant source material such as organic matter into microorganisms that inhabit the treatment target water area, and further to generate gentle convection in the treatment target water area. Without destroying the water, it is possible to exert the purification action of the water quality originally provided by the microorganisms living in the water area to be treated. Furthermore, the present invention enables continuous and continuous processing at a low cost.

FIG. 1 is a schematic diagram showing an outline of a water purification treatment apparatus of the present invention. FIG. 2 is a diagram showing an overview of Yazaemon Pond. FIG. 3 is a graph showing the change in chemical oxygen demand (COD) from April 2013 to April 2014 of Yazaemon Pond measured in Test Example 3-1. FIG. 4 is a graph showing the change in total carbon content (TC) from April 2013 to April 2014 of Yazaemonike measured in Test Example 3-1. FIG. 5 is a graph showing a change in total nitrogen amount (TN) of Yazaemon Pond measured from Test Example 3-1, from April 2013 to April 2014. FIG. 6 is a diagram showing a band pattern of electrophoresis by PCR-DGGE method performed in Test Example 3-3. FIG. 7 is a diagram showing the results of cluster analysis of the band pattern of electrophoresis by the PCR-DGGE method performed in Test Example 3-3. FIG. 8 is a diagram illustrating an outline of a circulation path and a water purification treatment apparatus laid in Yazaemon Pond in Test Example 3-4. FIG. 9 is a schematic diagram illustrating a part of the water purification apparatus in Test Example 3-4.

  Hereinafter, the present invention will be described in detail. In addition, it demonstrates with reference to an accompanying drawing as needed.

Water purification apparatus Figure 1 is a schematic diagram showing an outline of a water purification apparatus 1 according to an embodiment of the present invention. As shown in FIG. 1, a water purification treatment apparatus 1 includes an aerobic microorganism treatment unit 2 having a microorganism-adsorbing porous carrier, and a first circulation path 3 that introduces and discharges water to be treated into the aerobic microorganism treatment unit 2. A first pumping means 4 that is connected to the first circulation path 3 and pumps the water to be treated to the aerobic microorganism treatment unit 2; an anaerobic microorganism treatment unit 5 having a microorganism-adsorbing porous carrier; A second circulation path 6 that introduces and discharges water to be treated into the microorganism treatment section 5 and a second pumping means 7 that is connected to the second circulation path 6 and that pumps the treatment target water to the anaerobic microorganism treatment section 5. And.

  By providing the above-described configuration in the water purification treatment apparatus 1, the treatment target water is introduced from the treatment target water area 11 into the first circulation path 3 by the first pumping means 4 and passes through the aerobic microorganism treatment unit 2. Then, it is discharged to the treatment target water area 11 through the first circulation path 3. Similarly, the water is introduced from the treatment target water area 11 into the second circulation path 6 by the second pumping means 7, passes through the second circulation path 6 through the anaerobic microorganism treatment unit 5, and is discharged to the treatment target water area 11. Is done. In the water purification treatment apparatus 1, such a series of treatment target water can be continuously introduced and discharged.

  As long as the aerobic microorganism processing unit 2 and the anaerobic microorganism processing unit 5 have a microorganism-adsorbing porous carrier therein, the shape, material, size and the like are not particularly limited. Examples of the aerobic microorganism processing unit 2 and the anaerobic microorganism processing unit 5 include a column packed with a microorganism-adsorbing porous carrier. When a column packed with a microorganism-adsorbing porous carrier is used, the number and length of the columns can be appropriately adjusted depending on the degree of contamination in the water area to be treated and the composition of the contaminant. For example, when treating a water area rich in carbon compounds, the number of columns constituting the aerobic microorganism treatment unit 2 is increased, and when treating a water area rich in nitrogen compounds, anaerobic microorganism treatment is performed. The number of columns constituting the part 5 can be increased as appropriate.

The microorganism-adsorbing porous carrier contained in the aerobic microorganism treatment unit 2 and the anaerobic microorganism treatment unit 5 is a material that can pass water to be treated and can adsorb or carry microorganisms contained in the water to be treated. If there is no particular limitation. The microorganism-adsorbing porous carrier is preferably a material whose number of microorganisms that can be adsorbed or supported per gram of the carrier is approximately equal to or more than the number of microorganisms that exist per gram of general soil. Since it is known that about 1 × 10 ⁸ cells of microorganisms per gram exist in general soil, specifically, the number of microorganisms that can be adsorbed or supported per gram of microorganism-adsorbing porous carrier is 1 ^{× 10 7 ~1 × 10 10 cells} / g or so, it material is desired preferably ^{1 × 10 8 ~1 × 10 9} cells / g approximately. Examples of such materials include polymer resins such as polyvinyl alcohol resins, polyurethane resins, polyolefin resins, polyethylene glycol resins, and polyether resins. From the viewpoint of ease of handling, cost, etc. Alcohol resins are preferred. In addition, the polymerization degree and saponification degree of the polyvinyl alcohol resin are not particularly limited.

  The treatment target water is introduced from the treatment target water area 11 to the aerobic microorganism treatment unit 2 and the anaerobic microorganism treatment unit 5. The introduced water to be treated passes through the aerobic microorganism treatment unit 2 and the anaerobic microorganism treatment unit 5 while being in contact with the microorganism-adsorbing porous carrier, and microorganisms contained in the treatment-target water become microorganism-adsorbing porous carriers. Adsorbed or supported. In addition, a part of the microorganisms adsorbed or supported on the microorganism-adsorbing porous carrier is separated from the microorganism-adsorbing porous carrier and discharged into the treatment-target water area 11 due to a flow of water to be treated that passes through the processing unit.

  The microorganisms adsorbed or supported on the microorganism-adsorbing porous carrier include various microorganisms that live in the water area 11 to be treated. In the aerobic microorganism treatment unit 2, aerobic conditions (that is, oxygen is present). Abundant conditions), aerobic microbe activation (microorganism growth, etc.) occurs, and the anaerobic microbe treatment unit 5 is anaerobic conditions (that is, conditions in which oxygen is very poor), Activation of anaerobic microorganisms occurs. Substances that contribute to water pollution such as organic matter contained in the water to be treated by such microorganism activation are aerobically decomposed by aerobic microorganisms in the aerobic microorganism treatment unit 2 and treated with anaerobic microorganisms. In part 5, it is decomposed anaerobically.

  Moreover, the aerobic environment in the aerobic microorganism processing unit 2 and the anaerobic environment in the anaerobic microorganism processing unit 5 can be created by adjusting the oxygen concentration in each processing unit. For example, adjusting the flow rate per unit time of water to be treated that is pumped to each processing unit by the first pumping unit 4 and the second pumping unit 7, which will be described later, or air to the aerobic microorganism processing unit 2 By introducing the oxygen concentration, the oxygen concentration in each processing unit can be adjusted. In this specification, “aerobic environment” and “anaerobic environment” are not strictly defined by the oxygen concentration in the system. Is an aerobic environment, and an oxygen concentration is relatively low. That is, comparing both processing units, a processing unit considered to have a relatively high oxygen concentration is referred to as an aerobic microorganism processing unit, and a processing unit considered to have a relatively low oxygen concentration is referred to as an anaerobic microorganism processing unit.

  The first pumping means 4 and the second pumping means 7 are respectively connected to the first circulation path 3 and the second circulation path 6, and the first circulation path 3 and the second circulation from the processing target water area. As long as the water to be treated can be pumped to each of the paths, the size and type thereof are not particularly limited, and any of the circulation paths may be connected. A pump etc. are mentioned as an example of a pumping means.

  The flow rate (flow velocity) per unit time of the water to be treated that is pumped by the first pumping means 4 and the second pumping means 7 is appropriately set according to the area and volume of the water area to be treated, the size of each treatment section, and the like. be able to. When the flow rates per unit time of the processing target water pumped by the first pumping unit 4 and the second pumping unit 7 are different from each other, in the processing unit into which the processing target water having a large flow rate per unit time is introduced, Compared to the treatment unit into which the water to be treated having a small flow rate per unit time is introduced, a larger amount of oxygen is supplied. For this reason, the treatment unit into which the treatment target water having a large flow rate per unit time is introduced becomes an aerobic environment, and the treatment unit into which the treatment target water having a small flow rate per unit time is introduced becomes an anaerobic environment. Therefore, it is preferable that the flow rate per unit time of the processing target water pumped by the first pumping unit 4 is larger than the flow rate per unit time of the processing target water pumped by the second pumping unit 7.

  For example, when water in a 200 L water tank is used as the processing target water area, the flow rate per unit time of the processing target water pumped by the first pumping unit 4 is set to about 3 L / min, and the second pumping unit 7 is used. By setting the flow rate per unit time of the water to be treated that is pumped by about 2.4 L / min, the aerobic microorganism processing unit 2 is set to an aerobic environment and the anaerobic microorganism processing unit 5 is set to an anaerobic environment. be able to.

  The water purification treatment apparatus 1 can be provided with air introduction means for introducing air into the aerobic microorganism treatment unit 2 as necessary. By providing the air introduction means, it is possible to introduce air into the aerobic microorganism treatment unit 2 and to make the aerobic microorganism treatment unit 2 more aerobic. The shape, material, size, etc. of the air introduction means are not particularly limited as long as air can be introduced into the aerobic microorganism treatment unit 2. Examples of the air introduction means include an aeration pump and an air introduction tank 9 shown in FIG. An aeration pump and an air introduction tank may be combined. The air introduction means may be connected or installed anywhere as long as air can be introduced into the aerobic microorganism treatment unit 2, for example, connected to or installed in the first circulation path 3 or the aerobic microorganism treatment unit 2 itself. be able to. Furthermore, when the air introduction tank 9 is used as the air introduction means, the oxygen concentration of the treatment target water can be improved by the free fall of the treatment target water by designing the treatment target water to fall freely in the tank. it can.

  Moreover, the water quality purification apparatus 1 can be provided with a pumping control unit that stops the pumping of the water to be treated to the anaerobic microorganism processing unit 5 by the second pumping unit 7 for a predetermined period, if necessary. By stopping the pumping of the processing target water to the anaerobic microorganism processing unit 5 by the pumping control means for a predetermined period, the introduction of the processing target water to the anaerobic microorganism processing unit 5 is stopped for a predetermined period. That is, since the supply of oxygen to the anaerobic microorganism 5 is stopped for a predetermined period, the anaerobic microorganism treatment unit 5 can be made more anaerobic. Thus, the “stagnation” part in the natural water area can be reproduced by stopping the pumping of the water to be treated to the anaerobic microorganism treatment part 5 for a predetermined period. In addition, it does not restrict | limit especially as a period which stops the pumping of process target water, For example, a stop period can be adjusted in 1-59 minutes within 1 hour.

  The shape, material, size, etc. of the pressure feed control means are not particularly limited as long as the pressure feed water to be treated to the anaerobic microorganism treatment unit 5 can be stopped for a predetermined period. In addition, the pumping control unit may be connected or installed anywhere as long as the pumping of the water to be treated to the anaerobic microorganism processing unit 5 can be stopped for a predetermined period. For example, the pumping control unit may be connected to the second pumping unit 7 or Can be installed.

  The shape, material, size, etc. of the first circulation path 3 and the second circulation path 6 are not particularly limited as long as the water to be treated can be introduced and discharged. For example, the first circulation path 3 and the second circulation path 6 can be installed so that the treatment target water can be directly introduced from the treatment target water area.

  By introducing and discharging the treatment target water from the treatment target water area by the first circulation path 3 and the second circulation path 6, it is possible to generate a gentle convection in the treatment target water area. In order to make convection more likely to occur, the introduction port in the circulation path can be installed at the bottom of the treatment target water area, and the discharge port in the circulation path can be installed near the water surface of the treatment target water area. In this way, by installing the circulation path, the water to be treated is sucked up from the bottom of the water area to be treated and discharged near the surface of the water area to be treated, thereby causing convection in the depth direction (vertical direction) in the water area to be treated. Can be generated.

  Moreover, the 3rd circulation path 8 is installed, and it can also connect so that the to-be-processed water which flows through the 3rd circulation path 8 can be introduce | transduced into the 1st circulation path 3 and the 2nd circulation path 6. The introduction of the water to be treated from the water area 11 to be treated to the third circulation path 8 can be performed by, for example, the third pumping means 15. In particular, when a large water area such as a lake or a pond is to be treated, it is preferable that the water to be treated is circulated through the third circulation path 8 so that convection can be generated in the water area to be treated. In this case, in the first circulation path 3 and the second circulation path 6, for example, only the introduction port can be connected to the third circulation path 8, and both the introduction port and the discharge port can be connected to the third circulation path. It can also be connected to the path 8. Further, as shown in FIG. 9, the third circulation path 8 and the water storage section 12 are connected by the water passage 13, and a part of the processing target water flowing through the third circulation path 8 is temporarily stored in the water storage section 12. Can be stored in the water. Then, the water to be treated can be introduced from the water storage part 12 to each treatment part through the first circulation path and / or the second circulation path. In this case, in the first circulation path and / or the second circulation path, only the introduction port can be connected to the water storage unit 12, or both the introduction port and the discharge port can be connected to the water storage unit 12. . When both the introduction port and the discharge port are connected to the water storage unit 12, as shown in FIG. 9, the water storage unit 12 is provided with a discharge port 14 and the water level in the water storage unit 12 becomes a certain level or more. In addition, the water to be treated may be discharged to the water area 11 to be treated, or the water storage unit 12 may be provided with a discharge path, and the water to be treated can be discharged to the water area 11 to be treated through the discharge path. .

  The target water area to be treated by the water purification treatment apparatus 1 is not particularly limited, but water inflows and inflows are limited, and closed water areas such as lakes, ponds, dams, ponds, and reservoirs, and water tanks such as goldfish and aquatic plants are rare. This is especially effective when targeting aquariums for rearing organisms. By using the water purification treatment apparatus 1 in such a water area, microorganisms living in the water area can be activated, and further, gentle convection can be generated in the water area. In this specification, water areas where water inflow and outflow are scarce, such as closed water areas such as lakes, ponds, dams, reservoirs, and reservoirs, and aquariums for breeding aquatic organisms such as goldfish and aquatic plants. It is described as a water stop area.

Water purification treatment method The water purification treatment method of the present invention introduces (a) water to be treated into an aerobic microorganism treatment section having a microorganism-adsorbing porous carrier and an anaerobic microorganism treatment section having a microorganism-adsorption porous carrier, respectively. A step, (b) subjecting the water to be treated introduced in the step (a) to a decomposition treatment with an aerobic microorganism in the aerobic microorganism treatment unit, and a decomposition treatment with an anaerobic microorganism in the anaerobic microorganism treatment unit. A unit time of treatment target water introduced into the aerobic microorganism treatment unit, including a step of proceeding, and (c) discharging the treatment target water from the aerobic microorganism treatment unit and the anaerobic microorganism treatment unit, respectively. The per unit flow rate is larger than the flow rate per unit time of the water to be treated introduced into the anaerobic treatment unit.

  In the step (a), the water to be treated is introduced into the aerobic microorganism processing unit having the microorganism-adsorbing porous carrier and the anaerobic microorganism processing unit having the microorganism-adsorbing porous carrier, respectively. The treatment water to be introduced into each treatment unit can be performed, for example, by connecting a pipe or the like through which treatment target water can be passed to each treatment unit and pumping up treatment target water from the treatment target water area by a pump or the like. .

  In the step (b), the water to be treated introduced in the step (a) is subjected to a decomposition treatment with an aerobic microorganism in the aerobic microorganism treatment unit, and a decomposition treatment with an anaerobic microorganism in the anaerobic microorganism treatment unit. Make it progress. Water to be treated contains carbon-based compounds and nitrogen-based compounds that are nutrient sources for microorganisms, and these are decomposed aerobically or anaerobically to promote the growth of various microorganisms and to activate the microorganisms. Can do. In addition, it is preferable to use the microorganisms derived from the treatment target water area rather than the foreign microorganisms (that is, the microorganisms not derived from the treatment target water area). Further, the microorganisms in each processing unit may be adsorbed or supported in advance on the microorganism-adsorbing porous carrier, or may be adsorbed or supported on the microorganism-adsorbing porous carrier by introducing the water to be processed into each processing unit. .

  In the step (c), water to be treated is discharged from the aerobic microorganism treatment unit and the anaerobic microorganism treatment unit, respectively. At this time, the water to be treated contains substances decomposed in each treatment section and microorganisms adsorbed or carried on each treatment section, and these are discharged into the treatment target water area to treat the water. Decomposition treatment with microorganisms is also performed in the target water area.

  Further, by introducing and discharging the water to be treated from the water area to be treated by the steps (a) and (c), a gentle convection can be generated in the water area to be treated. By causing gentle convection, microorganisms (indigenous microorganisms) that live in the water area to be treated can be activated.

  Further, in the water purification treatment method of the present invention, the flow rate per unit time of the treatment target water introduced into the aerobic microorganism treatment unit is greater than the flow rate per unit time of the treatment target water introduced into the anaerobic treatment unit. It is also characterized by many. This is because, as described above, the treatment unit into which the treatment target water having a high flow rate per unit time is introduced has a larger amount of oxygen than the treatment unit into which the treatment target water having a low flow rate per unit time is introduced. Will be supplied. Therefore, the treatment part into which the treatment target water having a high flow rate per unit time is introduced can be set as an aerobic environment, and the treatment part into which the treatment target water with a low flow rate per unit time is introduced can be set as an anaerobic environment. .

  Furthermore, the water purification treatment method of the present invention can introduce air into the aerobic microorganism treatment unit in order to make the aerobic microorganism treatment unit more aerobic. In addition, in order to make the anaerobic microorganism treatment unit a more anaerobic environment, the introduction of water to be treated into the anaerobic microorganism treatment unit can be stopped for a predetermined period. Specific examples of these means include the air introduction means and the pressure feed control means described above.

  Furthermore, the water purification treatment method of the present invention introduces treatment target water from the treatment target water area into the circulation path, and discharges the treatment target water through the circulation path, thereby allowing large water areas such as lakes and ponds. Even if it is set as a process target water area, a convection can be produced in a process target water area. In this case, in the step (a), treatment target water flowing through the circulation path is introduced into each treatment section, and in the step (c), the treatment target water that has passed through each treatment section is discharged to the circulation path. Or, it can be discharged directly to the target water area. Moreover, in the said (c) process, after discharging process target water to a water storage part, process target water can also be discharged | emitted from this water storage part to a process target water area.

  EXAMPLES Hereinafter, although an Example is given and this invention is demonstrated further in detail, this invention is not limited to the following example.

Test Example 1: Treatment of simulated wastewater with high nitrogen content Assuming wastewater with high nitrogen content, simulated wastewater (15 L) obtained by diluting a synthetic sewage medium having the composition shown in Table 1 below with tap water 300 times The apparatus shown in FIG. 1 was operated for 7 days, and the chemical oxygen demand (COD) of simulated wastewater before and after 7 days (after treatment) was measured by the following method. Hereinafter, an example using the water purification treatment apparatus shown in FIG.

  In this test example, the flow rate per unit time of the processing target water pumped by the first pumping unit 4 shown in FIG. 1 is 300 mL / min, and the processing target water pumped by the second pumping unit 7. The flow rate per unit time was set to be 85 mL / min. Further, in the aerobic microorganism processing unit 2 and the anaerobic microorganism processing unit 5 shown in FIG. 1, columns filled with polyvinyl alcohol resin as a microorganism-adsorbing porous carrier were used. Furthermore, the aerobic microorganism treatment section 2 shown in FIG. 1 has one column (column inner diameter: 50 mm, column length: 200 mm, column capacity: 380 ml) packed with a polyvinyl alcohol-based resin as a microorganism-adsorbing porous carrier, One column was used for each anaerobic microorganism treatment section.

  Furthermore, as an object to be compared, an example in which an apparatus was not used (Comparative Example 1-1), and an apparatus using an apparatus that does not have an aerobic microorganism treatment unit 2 and an anaerobic microorganism treatment unit 5 among the devices shown in FIG. (Comparative Example 1-2), an example using an apparatus that does not have the anaerobic microorganism treatment unit 5 (Comparative Example 1-3), and an example that uses an apparatus that does not have the aerobic microorganism treatment unit 2 (Comparative Example 1- A similar test was conducted for 4).

<Measuring method of chemical oxygen demand (COD)>
100 mL of each sample water was placed in a 300 mL Erlenmeyer flask, 0.2 g of silver nitrate, 10 mL of concentrated sulfuric acid, and 10 mL of aqueous potassium permanganate solution were added, and heated in a water bath at 100 ° C. for 30 minutes. After heating, 10 mL of an aqueous sodium oxalate solution (factor (f) = 1.005) was further added, titrated with an aqueous potassium permanganate solution, and the chemical oxygen demand (COD) was calculated using the following formula.

  Table 2 below shows changes in COD before and after the treatment in Example 1 and Comparative Examples 1-1 to 1-4.

  As is apparent from Table 2, when the water purification apparatus shown in FIG. 1 is used (Example 1), COD is greatly reduced as compared with Comparative Examples 1-1 to 1-4. Was confirmed. From this result, in order to purify water with a high nitrogen content, a gentle convection is generated and treatment with microorganisms is performed using an apparatus equipped with both an aerobic microorganism treatment unit and an anaerobic microorganism treatment unit. I found it important. In particular, it has been found that by providing both the aerobic microorganism treatment unit and the anaerobic microorganism treatment unit, the purification treatment is promoted by the synergistic effect of the treatment with the aerobic microorganism and the treatment with the anaerobic microorganism.

Test example 2: Purification treatment test of simulated water stoppage with a high carbon content Assuming a water stoppage with a high carbon content, the test stop 1 and the simulated still water environment water having the composition shown in Table 3 below (15 L) Similarly, the water purification treatment apparatus shown in FIG. 1 was operated for 7 days, and the chemical oxygen demand (COD) of simulated still water environment water before and after treatment (after treatment) was measured. In the following, an example using the apparatus shown in FIG.

  Further, in the same manner as in Test Example 1, as an object to be compared, an aerobic microorganism processing unit 2 and an anaerobic microorganism processing unit 5 in the example in which the apparatus was not used (Comparative Example 2-1) and the apparatus shown in FIG. An example using a device that does not have an anaerobic (Comparative Example 2-2), an example using a device that does not have an anaerobic microbial treatment unit 5 (Comparative Example 2-3), and a device that does not have an aerobic microbial treatment unit 2 A similar test was performed on the used example (Comparative Example 2-4).

  Table 4 below shows changes in COD before and after the treatment in Example 2 and Comparative Examples 2-1 to 2-4.

  As is apparent from Table 4, when the apparatus shown in FIG. 1 is used (Example 2), it can be confirmed that COD is greatly reduced as compared with Comparative Examples 2-1 to 2-4. It was. Based on the results, in order to purify water with a high carbon content, a gentle convection is generated and treatment with microorganisms is performed using an apparatus equipped with both an aerobic microorganism treatment unit and an anaerobic microorganism treatment unit. I found it important. In particular, it has been found that by providing both the aerobic microorganism treatment unit and the anaerobic microorganism treatment unit, the purification treatment is promoted by the synergistic effect of the treatment with the aerobic microorganism and the treatment with the anaerobic microorganism.

Test example 3-1: Water quality survey in a natural still area From April 16, 2013 to April 14, 2014, the water at Yazaemon Pond in Ritsumeikan University Biwako-Kusatsu campus is about every two weeks. A water quality survey was conducted by measuring chemical oxygen demand (COD), total carbon (TC), and total nitrogen (TN). An overview of Yazaemon Pond is shown in Figure 2.

  The chemical oxygen demand (COD) is measured in the same manner as in Test Examples 1 and 2, and the total carbon (TC) and total nitrogen (TN) are measured using a total organic carbon meter (TOC-VCPH, Shimadzu Corporation) was used under the conditions shown in Table 5 below.

  The measurement results of chemical oxygen demand (COD), total carbon (TC), and total nitrogen (TN) are shown in Table 6 and FIGS.

Test Example 3-2: Water purification treatment test for actual stationary water area During the period from April 16, 2013 to April 14, 2014, 200 L of water was drawn from Yazaemon pond approximately every two weeks, and the water purification shown in FIG. After operating the treatment apparatus for 2 weeks, the chemical oxygen demand (COD), the total carbon content (TC), and the total nitrogen content (TN) were measured in the same manner as in Test Example 3-1.

  In this test example, the flow rate per unit time of the processing target water pumped by the first pumping unit 4 shown in FIG. 1 is 3 L / min, and the processing target water pumped by the second pumping unit 7 is used. The flow rate per unit time was set to 2.4 L / min. Moreover, in order to make the inside of the aerobic microorganism processing unit 2 shown in FIG. 1 a more aerobic environment, an air introduction means was connected to the first circulation path (not shown). Furthermore, in order to make the inside of the anaerobic microorganism processing unit 5 shown in FIG. 1 more anaerobic, the second pumping means pumps the water to be treated for 15 minutes and stops the pumping of the water to be treated for 45 minutes. The pressure feed control means to be connected was connected (not shown). Furthermore, the aerobic microorganism treatment section 2 shown in FIG. 1 has six columns (column inner diameter: 52 mm, column length: 347 mm) packed with polyvinyl alcohol resin as a microorganism-adsorbing porous carrier, and anaerobic microorganism treatment. Three parts of the same column were used for each part.

  The measurement results of chemical oxygen demand (COD) are shown in Table 7 below, the measurement results of total carbon content (TC) are shown in Table 8 below, and the measurement results of total nitrogen content (TN) are shown in Table 9 below. In Tables 7 to 9 below, for example, the COD, TC and TN measurement results after 2 weeks of water drawn on May 16, 2013 are described in the row “5/16”. Yes. Further, “COD before treatment”, “TC before treatment”, and “TN before treatment” in Tables 7 to 9 below describe data of the water quality investigation results performed in Test Example 3-1.

  As is clear from Tables 7 to 9, it was confirmed that COD, TC and TN were reduced by the treatment for 2 weeks. From this result, it is possible to effectively perform water treatment by generating a gentle convection and simultaneously performing treatment with microorganisms using an apparatus including both an aerobic microorganism treatment unit and an anaerobic microorganism treatment unit. It became clear. In particular, it has been found that by providing both the aerobic microorganism treatment unit and the anaerobic microorganism treatment unit, the purification treatment is promoted by the synergistic effect of the treatment with the aerobic microorganism and the treatment with the anaerobic microorganism.

Test Example 3-3: Analysis of microorganism group present in column <Analysis of the number of microorganisms by environmental DNA method (eDNA method)>
The number of microorganisms in the aerobic microorganism treatment unit 2 and the anaerobic microorganism treatment unit 5 in the purification treatment apparatus used in Test Example 3-2 (that is, the number of microorganisms supported on the polyvinyl alcohol-based resin) was determined using the following environmental DNA method. Analysis was performed by (eDNA method).

  In a 50 mL centrifuge tube, 1.0 g of polyvinyl alcohol resin in each processing section was weighed, and 8.0 mL of DNA extraction buffer (pH 8.0) having the composition shown in Table 10 below and 20% (w / v) dodecyl sulfate. Sodium solution (1.0 mL) was added, and the mixture was stirred at 1500 rpm for 20 minutes at room temperature. After stirring, a 1.5 mL sample was taken from a 50 mL centrifuge tube into a sterilized 1.5 mL microtube and centrifuged at 20 ° C. and 8000 rpm for 10 minutes. After centrifugation, 700 μL of the aqueous layer was taken into a new microtube, 700 μL of chloroform / isoamyl alcohol (24: 1, v / v) was added and mixed, and then centrifuged at 16 ° C. and 13000 rpm for 10 minutes. . After centrifugation, 500 μL of the aqueous layer was taken into a new microtube, 300 μL of 2-propanol was added, gently mixed, and centrifuged at 16 ° C. and 13000 rpm for 10 minutes. After centrifugation, the supernatant was removed, 500 μL of 70% (v / v) ethanol was added, and the mixture was centrifuged at 16 ° C. and 13000 rpm for 5 minutes. After centrifugation, the supernatant was removed and dried under reduced pressure with an aspirator for 30 minutes. Thereafter, 50 μL of a 10: 1 TE buffer solution (pH 8.0) having the composition shown in Table 11 below was added thereto, and the well-dissolved solution was used as an eDNA solution.

  Next, 1.0 μL of Loading Dye (manufactured by Toyobo Co., Ltd.) was mixed with 5.0 μL of the eDNA solution, and a total amount of 6.0 μL and 1.5 μL of Smart Ladder (manufactured by Nippon Gene) containing a known amount of DNA were added to the following table 12 was applied to each well of a 1.0% agarose gel having the composition shown in FIG. 12, and electrophoresis was performed at 100 V for 40 minutes. Thereafter, UV was directed to the agarose gel, and fluorescence intensity was measured with ethidium bromide as a label by KODAK1D Image Analysis software (manufactured by KODAK). A calibration curve of the DNA amount with respect to the fluorescence intensity of the band is created from the measured value of the fluorescence intensity of the Smart Ladder, and based on this, the DNA amount is obtained from the fluorescence intensity of the band of each sample DNA solution. The amount of eDNA per 0 g was calculated.

From the amount of eDNA calculated above, the relational expression Y = 1.7 × 10 ⁸ X (R ² = 0.96) converted to the total viable count by DAPI staining [Y: the number of microorganisms (cells / g-PVA), X: eDNA amount (g / g-PVA)] was used to calculate the number of microorganisms per 1.0 g of each polyvinyl alcohol resin. The calculation results are shown in Table 14 below.

As is clear from Table 14, the polyvinyl alcohol-based resin in the aerobic microorganism treatment part and the anaerobic microorganism treatment part carries 1 × 10 ⁹ (cells / g-PVA) or more microorganisms, It was confirmed that more microorganisms than the number of microorganisms in the soil environment (6 × 10 ⁸ (cells / g-soil)) were inhabited in the aerobic microorganism treatment part and the anaerobic microorganism treatment part. The results suggest that an aerobic microbial treatment unit and an anaerobic microbial treatment unit form a microbial environment that is close to or better than the natural environment, and that this allows efficient treatment. It was done.

<Analysis of microorganism group by PCR-DGGE method>
The microorganism group in the aerobic microorganism treatment unit 2 and the anaerobic microorganism treatment unit 5 in the water purification treatment apparatus used in Test Example 3-2 (that is, the microorganism group adsorbed on the polyvinyl alcohol-based resin) is expressed by the following PCR-DGGE. The analysis was performed by the (Degrading Gradient Gel Electrophoresis; denaturing agent concentration gradient gel electrophoresis) method.

  Using the eDNA solution obtained by the method of Test Example 3-3 as a template, the region containing the V3 region in the 16S rRNA gene of the microorganism was amplified by PCR reaction. The PCR solution shown in Table 15 below was added to a 200 μL tube, set in a thermal cycler (manufactured by BIOLAD), and PCR was performed under the conditions shown in Table 16 below. The base sequences of the primers used for PCR are shown in Table 17 below.

  16 mL of 20% and 40% denaturing agent acrylamide solutions having the composition shown in Table 18 below were prepared, and 150% of 10% (w / v) ammonium persulfate and N, N, N ′, N′-tetra were added to each solution. 15.0 μL of methylethylenediamine was added and mixed, and a gradient gel was prepared by a gradient maker and allowed to stand for 5 hours. Thereafter, the gradient gel was set in an electrophoresis tank filled with 7 L of 1 × TAE buffer and heated to 60 ° C.

  Next, a mixed solution of 10 μL of each PCR product obtained above and 5 μL of Loading Buffer was applied to each well of the gradient gel, and electrophoresis was performed for 18 hours under conditions of 44.5 V and 60 ° C. After electrophoresis, the gradient gel was immersed in a cyber gold solution for 40 minutes, stained, and then irradiated with UV on a transilluminator, and a band pattern was photographed with KODAK 1D Image Analysis software (manufactured by KODAK). The band pattern is shown in FIG.

  Furthermore, the band similarity index (BSI) was calculated by the following formula using the analysis software (Total Lab TL120 v2006, Nonlinea dynamics) for the band pattern photographed above. Further, cluster analysis was performed from the BSI by the Unweighted Pair Group Method with Arithmetic Mean (UPGMA) method. The results of cluster analysis of the band pattern are shown in FIG.

  As is clear from FIG. 6, it was confirmed that the aerobic microorganism processing unit and the anaerobic microorganism processing unit have different band patterns. Further, as apparent from FIG. 7, as a result of the band pattern cluster analysis, the similarity coefficient between the aerobic microorganism treatment part (lanes 2 and 3) and the anaerobic microorganism treatment part (lanes 4 and 5) is about 40%. Therefore, it was confirmed that the bacterial flora is different between the anaerobic microorganism treatment part and the anaerobic microorganism treatment part.

  As described above, the reason why the anaerobic microorganism treatment unit and the anaerobic microorganism treatment unit are different is considered as follows. In the anaerobic microorganism treatment section, dissolved oxygen concentration is relatively high near the water inlet, but oxygen is immediately consumed by microorganisms living near the inlet because the flow rate of water passing through the treatment section is slow. It is considered that the dissolved oxygen concentration in the entire anaerobic microorganism treatment unit is extremely low due to the stop of the second pumping means. On the other hand, since water always flows in the aerobic microorganism treatment part and the flow rate is fast, it is considered that the dissolved oxygen concentration in the aerobic microorganism treatment part is relatively high. Therefore, there are mainly aerobic microorganisms and facultative anaerobic microorganisms in the aerobic microorganism treatment part, and mainly facultative anaerobic microorganisms and obligate anaerobic microorganisms in the anaerobic microorganism treatment part. Seems to be inhabited.

Test Example 3-4: Water Purification Treatment Test in Actual Stop Water Area As shown in FIG. 8, a circulation path and a water purification treatment device were laid in Yazaemon pond. The circulation path is connected to a pumping means for pumping water so that water in the Yazaemon pond is introduced from the “suction” position in FIG. 8 and discharged from the “discharge” position. In addition, in order to generate convection in the Yazaemon pond, water was introduced from the vicinity of the bottom of the water, and water was discharged to the surface of the water. Was placed near the water surface.) Furthermore, the flow rate per unit time of water pumped to the circulation path by the pumping means was set to 800 L / min. Furthermore, as shown in FIG. 8, a water purification apparatus is connected to the circulation path, and a part of the water flowing through the circulation path is introduced into the aerobic microorganism treatment section and the anaerobic microorganism treatment section. A part of the water purification apparatus 1 connected to the circulation path is shown in FIG. FIG. 9 shows an overview of the aerobic microorganism treatment unit 2 side in the water purification treatment apparatus 1 connected to the third circulation path 8. In the water purification treatment apparatus in FIG. 9, the treatment target water is introduced from the treatment target water area 11 to the third circulation path 8 by the third pumping means 15 and discharged. Thereby, a gentle convection can be generated in the water area 11 to be treated. Further, part of the water to be treated that flows through the third circulation path 8 passes through the water passage 13 and is introduced into the water reservoir 12. The processing target water stored in the water storage section 12 is introduced into the first circulation path 3 and the second circulation path 6 respectively, and passes through the aerobic microorganism processing section 2 and the anaerobic microorganism processing section 5, respectively. The part 12 is discharged. The water storage unit 12 is provided with a discharge port 14, and when the water level in the water storage unit 12 exceeds a predetermined water level, the processing target water is discharged to the processing target water area 11 through the discharge port 14. ing.

  In this test example, the flow rate per unit time of the processing target water pumped by the first pumping unit 4 is 8 L / min, and the flow rate per unit time of the processing target water pumped by the second pumping unit. Was set to 3 L / min. Further, in order to make the inside of the aerobic microorganism treatment unit 2 more aerobic, as shown in FIG. 9, an air introduction means 10 and an air introduction tank 9 are connected to the first circulation path. Furthermore, in order to make the inside of the anaerobic microorganism treatment part a more anaerobic environment, the second pumping means is connected to a pumping control means for pumping the processing target water for 15 minutes and stopping the pumping of the processing target water for 45 minutes. (Not shown). Furthermore, the aerobic microorganism treatment unit 2 includes six columns (column inner diameter: 50 mm, column length: 350 mm) packed with a polyvinyl alcohol resin as a microorganism-adsorbing porous carrier, and the anaerobic microorganism treatment unit. Three columns were used respectively.

  The above-described purification treatment apparatus was operated for about one month, and the dissolved oxygen concentration (DO) in the vicinity of the water surface and the bottom of the Yazaemon pond was measured using a dissolved oxygen meter (manufactured by Horiba Seisakusho). Further, the chemical oxygen demand (COD) was measured in the same manner as in Test Example 3-1. The measurement results of dissolved oxygen concentration (DO) and chemical oxygen demand (COD) are shown in Tables 20 and 21 below.

  As is clear from Table 20, the dissolved oxygen concentration increased remarkably both near the water surface and at the bottom of the Yazaemon pond compared to before the purification treatment, and the inside of the Yazaemon pond became an aerobic environment. It was confirmed that From this result, it is considered that the activity of aerobic microorganisms in Yazaemon Pond is activated and the purification process is in progress.

  As is apparent from Table 21, it was confirmed that the COD of Yazaemon Pond showed a stable value in the treatment test for one month. In particular, in summer (around July to September), it was confirmed that a relatively low value was exhibited despite the relatively high COD value, as is clear from the results of Test Example 3-1. In addition, in the summer, there was a foul odor considered to be caused by anaerobic microorganisms in the vicinity of Yazaemon Pond in summer, but no such odor was confirmed after the start of the purification treatment test.

Test Example 4: Goldfish breeding water purification treatment test 100L of Yazaemon Pond and 100L of water derived from other tanks were put in a tank filled with 40kg of sand and gravel. The goldfish was raised at room temperature for 39 days while purifying using the purification apparatus, and the chemical oxygen demand (0, 5, 10, 14, 17, 21, 28, 31 and 39 days after the start of breeding) COD) was measured by the same method as in Test Example 1. Further, as a goldfish bait, about 0.5 g of tetrafin (manufactured by Spectrum Blanc Japan Co., Ltd.) was given at intervals of 2 to 3 times a day.

  In this test example, the flow rate per unit time of the processing target water pumped by the first pumping means 4 shown in FIG. 1 is 6 L / min, and the processing target water pumped by the second pumping means 7. The flow rate per unit time was set to 3 L / min. Further, the aerobic microorganism processing unit 2 shown in FIG. 1 includes three columns (column inner diameter: 52 mm, column length: 347 mm) packed with polyvinyl alcohol resin as a microorganism-adsorbing porous carrier, and an anaerobic microorganism processing unit. In column 5, 6 of the same column were used.

  The measurement results of chemical oxygen demand after 0, 5, 10, 14, 17, 21, 28, 31 and 39 days from the start of breeding are shown in Table 22 below.

  As is clear from Table 22, it was confirmed that even in a water area where goldfish live, stable COD was exhibited for more than one month. Moreover, the turbidity of water was hardly confirmed. Furthermore, it was confirmed that COD started to decrease after 28 days from the start of the treatment. This is thought to be because the microbial flora in the microbial treatment unit was stabilized, and the purification ability by convection with the microbial treatment unit was improved against water pollution caused by goldfish food or feces.

DESCRIPTION OF SYMBOLS 1 Water purification processing apparatus 2 Aerobic microorganism processing part 3 1st circulation path 4 1st pressure feeding means 5 Anaerobic microorganism processing part 6 2nd circulation path 7 2nd pressure feeding means 8 3rd circulation path 9 Air introduction Tank 10 Air introduction means 11 Processing target water area 12 Water storage part 13 Water passage 14 Discharge port 15 Third pumping means

Claims (7)

An aerobic microorganism treatment unit having a microorganism-adsorbing porous carrier;
A first circulation path for introducing and discharging water to be treated into the aerobic microorganism treatment section;
A first pumping means connected to the first circulation path and pumping the water to be treated to the aerobic microorganism treatment unit;
An anaerobic microorganism treatment section having a microorganism-adsorbing porous carrier;
A second circulation path for introducing and discharging water to be treated into the anaerobic microorganism treatment unit;
A second pumping means connected to the second circulation path and pumping the water to be treated to the anaerobic microorganism treatment unit;
Air introduction means for introducing air into the aerobic microorganism treatment section;
A third circulation path for introducing and discharging the water to be treated;
A water purification treatment apparatus comprising: a third pumping means connected to the third circulation path and pumping the water to be treated;
The flow rate per unit time of the processing target water pumped by the first pumping means is greater than the flow rate per unit time of the processing target water pumped by the second pumping means;
The flow rate per unit time of the water to be treated that is pumped by the first pumping unit is set to such an amount that the aerobic microorganism treatment unit maintains an aerobic environment, and is pumped by the second pumping unit. The flow rate per unit time of the water to be treated is such an amount that the anaerobic microorganism treatment unit maintains an anaerobic environment,
The introduction port and the discharge port of the third circulation path are provided in a common water area to be treated, the introduction ports of the first circulation path and the second circulation path are connected to a third circulation path, and the first circulation path A water purification treatment apparatus , characterized in that the circulation path and the discharge port of the second circulation path are connected to a third circulation path or are provided in the treatment target water area . An aerobic microorganism treatment unit having a microorganism-adsorbing porous carrier;
A first circulation path for introducing and discharging water to be treated into the aerobic microorganism treatment section;
A first pumping means connected to the first circulation path and pumping the water to be treated to the aerobic microorganism treatment unit;
An anaerobic microorganism treatment section having a microorganism-adsorbing porous carrier;
A second circulation path for introducing and discharging water to be treated into the anaerobic microorganism treatment unit;
A second pumping means connected to the second circulation path and pumping the water to be treated to the anaerobic microorganism treatment unit;
Air introduction means for introducing air into the aerobic microorganism treatment section;
A third circulation path for introducing and discharging the water to be treated;
A third pumping means connected to the third circulation path and pumping the water to be treated;
A water purification treatment apparatus comprising: a water storage part for storing treatment target water introduced from the third circulation path ,
The flow rate per unit time of the processing target water pumped by the first pumping means is greater than the flow rate per unit time of the processing target water pumped by the second pumping means;
The flow rate per unit time of the water to be treated that is pumped by the first pumping unit is set to such an amount that the aerobic microorganism treatment unit maintains an aerobic environment, and is pumped by the second pumping unit. The flow rate per unit time of the water to be treated is such an amount that the anaerobic microorganism treatment unit maintains an anaerobic environment,
The third circulation path and the water reservoir are connected by a water passage;
The introduction port and the discharge port of the third circulation path are provided in a common treatment target water area,
The inlet of the first circulation path and the second circulation path is connected to the water storage section, and the discharge port of the first circulation path and the second circulation path is connected to the water storage section, or Provided in the target water area,
Water in the water storage section is characterized by being discharged to the processed water from the outlet of the reservoir, the water purification apparatus. The water purification processing apparatus according to claim 1 or 2, further comprising: a pumping control unit that stops pumping of the processing target water to the anaerobic microorganism processing unit for a predetermined period. Wherein the microorganism adsorbed porous carrier comprises a polyvinyl alcohol resin, a polyurethane resin, a polyolefin resin, at least one resin selected from the group consisting of polyethylene glycol-based resin and a polyether-based resin, according to claim 1 to 3 The water treatment apparatus as described in any one of. (A) introducing water to be treated into an aerobic microorganism treatment unit having a microorganism-adsorbing porous carrier and an anaerobic microorganism treatment unit having a microorganism-adsorbing porous carrier,
(B) a step of advancing decomposition treatment with aerobic microorganisms in the aerobic microorganism treatment unit and decomposition treatment with anaerobic microorganisms in the anaerobic microorganism treatment unit with respect to the water to be treated introduced in the step;
(C) discharging the water to be treated from the aerobic microorganism treatment unit and the anaerobic microorganism treatment unit, and
(D) the processed water from the processed water was introduced into the circulation path, by the water to be treated is passed through the circulation path is discharged to the processed body of water, the step of causing the convection in the process target in the body of water Including
The flow rate per unit time of water to be treated introduced into the aerobic microorganism treatment unit is greater than the flow rate per unit time of water to be treated introduced into the anaerobic microorganism treatment unit,
Performing introduction of water to be treated into the aerobic microorganism treatment unit and the anaerobic microorganism treatment unit using different pumping means;
The flow rate per unit time of the water to be treated that is pumped by the pumping means is set to such an amount that the aerobic microorganism treatment unit maintains an aerobic environment, and the anaerobic microorganism treatment unit maintains an anaerobic environment. Such an amount,
Introducing air together with water to be treated into the aerobic microorganism treatment section,
In the step (a), introducing the processed water from the circulation passage, and in the step (c), after discharging the processed water to the reservoir, the water being treated to the processing target water area from the water storage section Characterized by discharging ,
Water purification treatment method. 6. The water purification method according to claim 5 , wherein the introduction of water to be treated into the anaerobic microorganism treatment unit is stopped for a predetermined period. Wherein the microorganism adsorbed porous carrier, polyvinyl alcohol resin, including polyurethane resins, polyolefin resins, at least one resin selected from the group consisting of polyethylene glycol-based resin and a polyether-based resin, according to claim 5 or 6 The water purification treatment method according to 1.