JP6230349B2 - Culture solution for bathing treatment and circulation device for culture solution - Google Patents
Culture solution for bathing treatment and circulation device for culture solution Download PDFInfo
- Publication number
- JP6230349B2 JP6230349B2 JP2013190646A JP2013190646A JP6230349B2 JP 6230349 B2 JP6230349 B2 JP 6230349B2 JP 2013190646 A JP2013190646 A JP 2013190646A JP 2013190646 A JP2013190646 A JP 2013190646A JP 6230349 B2 JP6230349 B2 JP 6230349B2
- Authority
- JP
- Japan
- Prior art keywords
- bacillus
- bacteria
- culture solution
- oxygen
- temperature
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000003287 bathing Methods 0.000 title claims description 24
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 68
- 229910052760 oxygen Inorganic materials 0.000 claims description 68
- 239000001301 oxygen Substances 0.000 claims description 68
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 61
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 51
- 239000000843 powder Substances 0.000 claims description 32
- 238000010438 heat treatment Methods 0.000 claims description 25
- 239000001963 growth medium Substances 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 7
- 241000894006 Bacteria Species 0.000 description 26
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 18
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 15
- 230000004060 metabolic process Effects 0.000 description 15
- 238000003756 stirring Methods 0.000 description 12
- 201000004624 Dermatitis Diseases 0.000 description 11
- 244000052616 bacterial pathogen Species 0.000 description 10
- 239000000126 substance Substances 0.000 description 9
- 230000000694 effects Effects 0.000 description 8
- 244000063299 Bacillus subtilis Species 0.000 description 7
- 235000014469 Bacillus subtilis Nutrition 0.000 description 7
- 238000005273 aeration Methods 0.000 description 7
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 7
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 6
- 241001148470 aerobic bacillus Species 0.000 description 6
- 230000007423 decrease Effects 0.000 description 6
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 6
- 239000002245 particle Substances 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 235000018102 proteins Nutrition 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 5
- LSDPWZHWYPCBBB-UHFFFAOYSA-N Methanethiol Chemical compound SC LSDPWZHWYPCBBB-UHFFFAOYSA-N 0.000 description 5
- 241000191967 Staphylococcus aureus Species 0.000 description 5
- 238000005187 foaming Methods 0.000 description 5
- 239000007789 gas Substances 0.000 description 5
- 239000008103 glucose Substances 0.000 description 5
- 238000002560 therapeutic procedure Methods 0.000 description 5
- 241000194107 Bacillus megaterium Species 0.000 description 4
- 238000013019 agitation Methods 0.000 description 4
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 4
- 238000000855 fermentation Methods 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000005416 organic matter Substances 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- 241000193752 Bacillus circulans Species 0.000 description 3
- 241000193422 Bacillus lentus Species 0.000 description 3
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 3
- 235000011511 Diospyros Nutrition 0.000 description 3
- 244000236655 Diospyros kaki Species 0.000 description 3
- 241000233866 Fungi Species 0.000 description 3
- 241001417534 Lutjanidae Species 0.000 description 3
- 241000555676 Malassezia Species 0.000 description 3
- 239000012531 culture fluid Substances 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 241000193755 Bacillus cereus Species 0.000 description 2
- 241000194108 Bacillus licheniformis Species 0.000 description 2
- 241000589248 Legionella Species 0.000 description 2
- 208000007764 Legionnaires' Disease Diseases 0.000 description 2
- 244000000231 Sesamum indicum Species 0.000 description 2
- 235000003434 Sesamum indicum Nutrition 0.000 description 2
- 230000009604 anaerobic growth Effects 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000036284 oxygen consumption Effects 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 235000019260 propionic acid Nutrition 0.000 description 2
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 2
- 208000037851 severe atopic dermatitis Diseases 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 244000000000 soil microbiome Species 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 244000223760 Cinnamomum zeylanicum Species 0.000 description 1
- 206010012438 Dermatitis atopic Diseases 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 241000123826 Lutjanus campechanus Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000009603 aerobic growth Effects 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- 201000008937 atopic dermatitis Diseases 0.000 description 1
- OVLHQUWQINALPR-UHFFFAOYSA-N azane;dichloromethane Chemical compound N.ClCCl OVLHQUWQINALPR-UHFFFAOYSA-N 0.000 description 1
- 239000013040 bath agent Substances 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 235000017803 cinnamon Nutrition 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000013021 overheating Methods 0.000 description 1
- 230000033116 oxidation-reduction process Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000007918 pathogenicity Effects 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Description
本発明は、皮膚炎を治療するための入浴治療に使用する入浴治療用の培養液及び浴槽に入浴治療用の培養液を循環させるための培養液循環装置に関する。 The present invention relates to a culture solution for bathing treatment used for bathing treatment for treating dermatitis and a culture solution circulating apparatus for circulating the culture solution for bathing treatment in a bathtub.
バチルス入浴療法（Ｂａｃｉｌｌｕｓ Ｓｐａ Ｔｈｅｒａｐｙ、以下ＢＳＴと略す）は人の皮膚の病原菌を有効Ｂａｃｉｌｌｕｓの作用により抑制すると同時に皮膚免疫に変化をもたらし皮膚疾患を改善させる療法である。水辺の植物である真菰等を発酵させることにより得られたバチルス芽胞を多量に含んだ粉末（バチルス粉末）を入浴剤として利用する。 Bacillus spa therapy (hereinafter abbreviated as BST) is a therapy that suppresses pathogenic bacteria in human skin by the action of effective Bacillus and at the same time changes skin immunity and improves skin diseases. A powder (Bacillus powder) containing a large amount of Bacillus spore obtained by fermenting red snapper, which is a waterside plant, is used as a bath agent.
以前より真菰の発酵粉末（例えば、特許文献１参照）を風呂のお湯に懸濁させ長期にわたり浴水を替えずに入浴する民間療法が一部の愛好者で行われていた。しかし効果はまちまちで科学的な機序の解明や水質管理がなされる事もなく悪臭や皮膚炎の悪化が頻回に生じ広く普及することはなかった。 Some enthusiasts have traditionally performed folk remedies in which a fermented powder of true potato (see, for example, Patent Document 1) is suspended in hot water in a bath and bathed without changing the bath water for a long period of time. However, the effects varied, and the scientific mechanism was not clarified and water quality control was not performed. Odor and dermatitis worsened frequently and did not spread widely.
ＢＳＴは本願発明者の８年間の臨牀研究から考え出された非常に有効なアトピー性皮膚炎の入浴療法であり多くの重症のアトピー性皮膚炎患者を救済する可能性が高い。
有効バチルス菌による病原性微生物の抑制と皮膚免疫の改善が最大の機序であり浴水中の微生物の管理及び老廃物の処理による水質の管理が最大の要素を持つ。
BST is a very effective bathing therapy for atopic dermatitis, which was conceived from the eight-year clinical study conducted by the inventor of the present application, and is highly likely to rescue many patients with severe atopic dermatitis.
Suppression of pathogenic microorganisms by effective Bacillus bacteria and improvement of skin immunity are the greatest mechanisms, and management of microorganisms in bath water and management of water quality by treatment of waste products have the greatest factors.
ところが、前述のように、ＢＳＴを行うために真菰の発酵粉末を浴槽のお湯に懸濁させ、長期にわたってお湯(培養液）を替えず、その水質管理を行わないままで入浴するという従来の入浴療法では、培養液の温度管理やエアレーションが行われないため、不要な雑菌や嫌気性菌が繁殖して悪臭が発生したり、有効バクテリアが減少し効果が低減したり、時には有害な病原性菌が繁殖したりするなど、培養液の状態を入浴治療に適した状態に保つことができないという問題があった。 However, as described above, in order to perform BST, traditional fermented powder is suspended in hot water in the bathtub, and bathing is performed without changing the hot water (culture solution) over a long period of time without controlling the water quality. In therapy, temperature control and aeration of the culture solution are not performed, so unwanted germs and anaerobic bacteria propagate to cause bad odor, reduce effective bacteria, reduce effectiveness, and sometimes harmful pathogenic bacteria There is a problem that the state of the culture solution cannot be maintained in a state suitable for bathing treatment.
本発明は、こうした問題に鑑みなされたもので、皮膚炎の治療に適した入浴治療用の培養液とその培養液を治療に適した状態に保つことができる入浴治療用の培養液循環装置とを提供することを目的とする。 The present invention has been made in view of these problems, and a culture solution for bathing treatment suitable for the treatment of dermatitis, and a culture solution circulation device for bathing treatment capable of maintaining the culture solution in a state suitable for treatment, The purpose is to provide.
この欄においては、発明に対する理解を容易にするため、必要に応じて「発明を実施するための形態」欄において用いた符号を付すが、この符号によって請求の範囲を限定することを意味するものではない。 In this column, in order to facilitate understanding of the invention, the reference numerals used in the “Mode for Carrying Out the Invention” column are attached as necessary, which means that the scope of claims is limited by this reference numeral. is not.
上記「発明が解決しようとする課題」において述べた問題を解決するためになされた発明は、１５０〜１８０リットルの浴水に約６００ｇのバチルス粉末を懸濁し、そのバチルス粉末を懸濁した浴水を３５〜４５℃の温度に保つとともに、酸素飽和度７０％以上（約１．０ｍｇ／ｍｉｎ／Ｌ以上）の酸素供給を行って数日間発酵させることにより得られることを特徴とする入浴治療用の培養液（バチルス浴水）である。 The invention made in order to solve the above-mentioned problems to be solved by the present invention is to suspend about 600 g of Bacillus powder in 150 to 180 liters of bath water, and bath water in which the Bacillus powder is suspended. For bathing treatment, characterized in that it is obtained by maintaining the temperature at 35 to 45 ° C. and fermenting for several days by supplying oxygen with an oxygen saturation of 70% or more (about 1.0 mg / min / L or more). Culture medium (Bacillus bath water).
このような入浴治療用の培養液を用いると、嫌気性の腐敗が生じることなく本来のバチルス属細菌の好気的繁殖が生じバチルス粉末内の有機物や入浴者の皮膚からの老廃物の代謝分解が行われる。そして、培養液内に１０6〜１０7ＣＦＵ／ｍｌ以上のバチルス属の有効細菌が効率よく培養され皮膚炎の原因菌である黄色ブドウ球菌や酵母様真菌であるマラセチアやカンジダを抑制することができる。 The use of such a bathing treatment medium results in aerobic growth of the original Bacillus bacteria without anaerobic spoilage, resulting in metabolic degradation of organic matter in the Bacillus powder and waste products from the bather's skin. Is done. Then, effective bacteria of the genus Bacillus of 10 6 to 10 7 CFU / ml or more are efficiently cultured in the culture solution to suppress staphylococcus aureus, yeast-like fungi, malassezia and candida. it can.
ここで、バチルス属細菌について説明する。バチルス属細菌は土壌中に広く生息し枯れた植物を分解する従属栄養細菌であり病原性は低い納豆菌もその１つである。
バチルス属細菌は、バチルス・サブティリス（Ｂａｃｉｌｌｕｓ ｓｕｂｔｉｌｌｉｓ）、バチルス・メガテリウム（Ｂａｃｉｌｌｕｓ ｍｅｇａｔｅｒｉｕｍ）、バチルス・レンタス（Ｂａｃｉｌｌｕｓ ｌｅｎｔｕｓ）のような 常に酸素を必要とする偏性好気性細菌とバチルス・サーキュランス（Ｂａｃｉｌｌｕｓ ｃｉｒｃｕｌａｎｓ）、バチルス・リケニフォルミス（Ｂａｃｉｌｌｕｓ ｌｉｃｈｅｎｉｆｏｒｍｉｓ）、バチルス・セレウス（Ｂａｃｉｌｌｕｓ ｃｅｒｅｕｓ）のように、酸素が利用できない環境では嫌気性代謝を行う通性好気性細菌が存在する。
Here, Bacillus bacteria will be described. Bacillus bacteria are heterotrophic bacteria that inhabit the soil and decompose dead plants, and one of them is Bacillus natto.
Bacteria belonging to the genus Bacillus subtilis (Bacillus subtilis), Bacillus megaterium (Bacillus megaterium), Bacillus lentus (Bacillis lentus) and obligately aerobic bacteria and Bacillus circulans (Bacillus circulus) that always require oxygen. There are facultative aerobic bacteria that perform anaerobic metabolism in an environment where oxygen is not available, such as circulans, Bacillus licheniformis, and Bacillus cereus.
酸素が少ない静水の底付近では、通性好気性細菌や嫌気性菌による嫌気性代謝が行われ人体に有害で悪臭原因ともなる硫化水素（Ｈ２Ｓ、メタン（ＣＨ４）、メルカプタン 低級脂肪酸のプロピオン酸やノルマル酪酸などが形成される。これらの悪臭物質は不快感のみならず人体に有害性があり皮膚炎を悪化させる原因となる。 Near the bottom of still water with little oxygen, anaerobic metabolism by facultative aerobic bacteria and anaerobic bacteria is performed, and hydrogen sulfide (H2S, methane (CH4), mercaptan, lower fatty acid propionic acid, which is harmful to human body and causes odor) It forms normal butyric acid, etc. These malodorous substances are harmful to the human body as well as unpleasant and cause dermatitis to worsen.
そこで、浴水１５０〜１８０Ｌに約６００ｇの真菰発酵粉末などのバチルス粉末を懸濁し ３５〜４５度の温度管理 酸素飽和度７０％以上の酸素供給と攪拌を行い、新たな発酵を生じさせる。条件が満たされると嫌気性の腐敗が生じることなく本来のバチルスの好気的繁殖が生じバチルス粉末内の有機物の代謝分解が行われる。 Therefore, about 600 g of Bacillus powder such as powdered snapper fermented in 150 to 180 L of bath water is suspended, temperature control of 35 to 45 degrees, oxygen supply with an oxygen saturation of 70% or more and stirring are performed to cause new fermentation. When the conditions are met, the anaerobic growth of the original Bacillus occurs without causing anaerobic decay and the organic matter in the Bacillus powder is metabolized.
また、条件が整うと浴水内に１０6〜１０7ＣＦＵ／ｍｌ以上のバチルス属の有効細菌が効率よく培養され皮膚炎の原因菌である黄色ブドウ球菌や酵母様真菌であるマラセチアやカンジダを抑制する。つまり、皮膚炎を治療するために適した培養液とすることができる。 In addition, when the conditions are met, effective bacteria of the genus Bacillus of 10 6 to 10 7 CFU / ml or more are efficiently cultured in the bath water, and the staphylococcus aureus and yeast-like fungi such as malassezia and candida. Suppress. That is, it can be set as the culture solution suitable for treating dermatitis.
ところで、培養液の活性が高いバチルス属菌数を維持し、真菰の発酵粉末のようなバチルス粉末、人体から排泄される脂肪あるいはタンパク質の代謝から産生される有害物質を減らすには、好気的な水質条件を維持する酸素供給（エアレーション）、沈殿しやすい懸濁粒子を常に攪拌し浴槽低層の嫌気条件を改善し有害物質の揮発を促すための攪拌（サーキュレーション）と２４時間培養温度を安全に維持できる温度管理（ヒーティング）が必要である。 By the way, it is aerobic to maintain the number of Bacillus spp. With high culture activity and reduce harmful substances produced from metabolism of fat or protein excreted from the human body, such as Bacillus powder such as a true fermented powder. Oxygen (aeration) to maintain proper water quality conditions, agitation (circulation) to improve the anaerobic conditions of the lower layer of the bath and promote volatilization of harmful substances, and safe 24 hour culture temperature Temperature management (heating) that can be maintained at a low temperature is required.
そこで、請求項２に記載のように、浴槽に入れた請求項１に記載の培養液を循環させるための循環手段（４０）と、循環手段（４０）により循環される培養液を加熱する加熱手段（５０）と、循環手段（４０）により循環される培養液中に酸素を供給する酸素供給手段（６０）と、循環手段（４０）により循環される培養液の温度を加熱手段（５０）により３５〜４５℃に加熱して保持させるとともに、酸素供給手段（６０）により培養液の酸素飽和度を７０％以上にする制御手段（７０）と、を備えた入浴治療用の培養液循環装置（１）とする。 Therefore, as described in claim 2, circulating means (40) for circulating the culture solution according to claim 1 in a bathtub, and heating for heating the culture solution circulated by the circulation means (40) Means (50), oxygen supply means (60) for supplying oxygen into the culture medium circulated by the circulation means (40), and heating means (50) for controlling the temperature of the culture liquid circulated by the circulation means (40). And a control means (70) for heating and holding at 35 to 45 ° C. by the oxygen supply means (60) and setting the oxygen saturation of the culture liquid to 70% or more by the oxygen supply means (60). (1).
このようにすると、培養液を循環手段（４０）で循環させることにより、バチルス粉末、人体から排泄される脂肪あるいはタンパク質の代謝から産生される有害物質を減少させるとともに、沈殿しやすい懸濁粒子を常に攪拌して浴槽低層の嫌気条件を改善することができる。 In this way, the circulating liquid is circulated by the circulation means (40) to reduce harmful substances produced from the metabolism of bacillus powder, fat or protein excreted from the human body, and suspension particles that easily precipitate. It can be constantly stirred to improve the anaerobic condition of the lower layer of the bathtub.
また、加熱手段（５０）により培養液の温度を３５〜４５℃に保持しつつ、酸素供給手段（６０）により培養液の酸素飽和度が７０％以上になるようにすると、培養液の活性が高いバチルス属細菌を維持することができる。 Moreover, when the oxygen saturation of the culture medium is set to 70% or more by the oxygen supply means (60) while the temperature of the culture liquid is maintained at 35 to 45 ° C. by the heating means (50), the activity of the culture liquid is increased. High Bacillus bacteria can be maintained.
つまり、皮膚炎の治療に適した入浴治療用の培養液を治療に適した状態に保つことができる入浴治療の培養液循環装置（１）とすることができる。
ところで、細菌は至適発育温度によって、１０〜２０℃：低温性菌、３０〜４０℃：中温性菌、５０〜６０℃：高温性菌の３つのグループに分類される。
That is, it is possible to provide a culture solution circulating apparatus (1) for bathing treatment that can maintain a bathing treatment culture solution suitable for dermatitis treatment in a state suitable for treatment.
By the way, bacteria are classified into three groups according to the optimum growth temperature: 10 to 20 ° C .: psychrophilic bacteria, 30 to 40 ° C .: mesophilic bacteria, and 50 to 60 ° C .: thermophilic bacteria.
病原制菌のほとんどが中温性菌に含まれる。一方、土壌菌であるバチルス属細菌は高温でも増殖が可能で芽胞形成能があり一旦芽胞を形成すると１００℃でも不活化できない。
そこで、制御手段（７０）によって、循環手段（４０）で循環される培養液の温度を加熱手段（５０）により５０℃に加熱し、所定時間保持するようにすると、例えば、夜間入浴後に、加熱手段（５０）を作動させておくと、翌日午前までに処理が終了し入浴温度となる。また、５０℃という温度は人が間違って接触しても軽微な低温熱傷で済み安全性も確保できる。
Most pathogenic bacteria are contained in mesophilic bacteria. On the other hand, Bacillus bacteria, which are soil bacteria, can grow at high temperatures and have spore-forming ability, and once formed, they cannot be inactivated even at 100 ° C.
Wherein, the control means (70), the temperature of the culture liquid to be circulated in a circulating means (40) was heated to 50 ° C. by the heating means (50), when to hold a predetermined time, for example, after the night bathing If the heating means (50) is operated, the treatment is completed by the morning of the next day and the bathing temperature is reached. In addition, a temperature of 50 ° C. requires only a slight low-temperature burn even if a person makes a mistake, and safety can be ensured.
以下、本発明が適用された実施形態について図面を用いて説明する。なお、本発明の実施の形態は、下記の実施形態に何ら限定されることはなく、本発明の技術的範囲に属する限り種々の形態を採りうる。
［培養液循環装置１の構成］
図１は、本発明が適用された入力治療用の培養液循環装置１の概略の外観図であり、図２は、培養液循環装置１の概略の構成を示すブロック図である。
Embodiments to which the present invention is applied will be described below with reference to the drawings. The embodiment of the present invention is not limited to the following embodiment, and can take various forms as long as they belong to the technical scope of the present invention.
[Configuration of Culture Medium Circulation Device 1]
FIG. 1 is a schematic external view of a culture fluid circulation apparatus 1 for input therapy to which the present invention is applied, and FIG. 2 is a block diagram showing a schematic configuration of the culture fluid circulation apparatus 1.
図１に示すように、培養液循環装置１は、本体１０及び泡出しユニット２０を備えている。
本体１０は、筐体１１及び蓋１２からなっている。そして、本体１０の下面には図示しない入水口、出水口及びエアホース接続口が設けられ、本体１０の正面には、操作パネル１４が設けられている。
As shown in FIG. 1, the culture medium circulating apparatus 1 includes a main body 10 and a foaming unit 20.
The main body 10 includes a housing 11 and a lid 12. A water inlet, a water outlet, and an air hose connection port (not shown) are provided on the lower surface of the main body 10, and an operation panel 14 is provided on the front surface of the main body 10.
入水口には、入水用ホース３０が接続され、浴槽の培養液を、入水用ホース３０を介して本体１０の内部に取り入れるようになっている。
出水口には、出水用ホース３２が接続され、入水口から取り入れた培養液を、後述する循環部４０で循環させるとともに、加熱部５０や酸素供給部６０により培養液の温度や温度を調整し酸素を供給した後出水用ホースを介して浴槽に戻すようになっている。
The water inlet hose 30 is connected to the water inlet, and the culture solution in the bathtub is taken into the main body 10 through the water inlet hose 30.
The outlet hose 32 is connected to the outlet, and the culture solution taken from the inlet is circulated in the circulation unit 40 described later, and the temperature and temperature of the culture solution are adjusted by the heating unit 50 and the oxygen supply unit 60. After supplying oxygen, it returns to the bathtub through a water discharge hose.
また、本体１０は、図２に示すように、その内部に、循環部４０、加熱部５０、酸素供給部６０、制御部７０を備えている。
循環部４０は、浴槽に入れた培養液を循環させるための装置であり、図示しないポンプにより、浴槽内部の培養液を、入水用ホース３０を介して入水口から吸い込み、吸い込んだ培養液を、循環させている。
As shown in FIG. 2, the main body 10 includes a circulation unit 40, a heating unit 50, an oxygen supply unit 60, and a control unit 70 therein.
The circulation unit 40 is a device for circulating the culture solution put in the bathtub, and sucks the culture solution inside the bathtub from the inlet through the water inlet hose 30 by a pump (not shown). Circulating.
循環部４０のポンプは、ブレードなどの回転部分を、回転軸を回転中心として回転させるタイプのものであるが、軸受を有する回転軸で回転部分を支持する従来の構造では、バチルス浴水中の懸濁粒子による摩擦負荷により支持部分が損傷しやすいため、軸受部分をなくしたマグネットタイプ（磁気軸受）を用いている。 The pump of the circulation unit 40 is of a type in which a rotating part such as a blade is rotated about a rotating shaft. However, in the conventional structure in which the rotating part is supported by a rotating shaft having a bearing, a suspension in the Bacillus bath water is used. Since the support part is easily damaged by the frictional load caused by the turbid particles, the magnet type (magnetic bearing) without the bearing part is used.
加熱部５０は、循環部４０により循環される培養液を加熱して保持する装置であり、図２に示すように、ヒータ５２と温度センサ５４を備えている。そして、循環部４０により本体１０内に吸い込まれた培養液の温度を温度センサ５４で検出し、制御部７０に出力するとともに、制御部７０からの信号により、培養液をヒータ５２で加熱して、本体１０の温度設定スイッチにより設定された温度（３５〜５０℃）に保つようになっている。 The heating unit 50 is a device that heats and holds the culture solution circulated by the circulation unit 40, and includes a heater 52 and a temperature sensor 54 as shown in FIG. Then, the temperature of the culture solution sucked into the main body 10 by the circulation unit 40 is detected by the temperature sensor 54 and output to the control unit 70, and the culture solution is heated by the heater 52 by a signal from the control unit 70. The temperature set by the temperature setting switch of the main body 10 (35 to 50 ° C.) is maintained.
ヒータ５２は培養液中の多量の浮遊粒子の焦げ付きにより熱伝導が阻害されオーバーヒートするため、熱伝導が穏やかなセラミックヒーターを使用しポンプで流速を維持しながら加熱することにより今まで頻回に生じていたオーバーヒートを回避できた。 Heater 52 is overheated because the heat conduction is hindered due to the burning of a large amount of suspended particles in the culture solution, so it has been generated frequently by using a ceramic heater with gentle heat conduction and heating while maintaining the flow rate with a pump. I was able to avoid overheating.
酸素供給部６０は、循環部４０により循環される培養液に酸素を供給する装置であり、制御部７０からの指令信号により培養液中の酸素飽和度が７０％以上になるように、培養液中に酸素を供給する。 The oxygen supply unit 60 is a device that supplies oxygen to the culture solution circulated by the circulation unit 40, and the culture solution is set so that the oxygen saturation in the culture solution becomes 70% or more by a command signal from the control unit 70. Supply oxygen inside.
なお、酸素供給部６０により供給される酸素の量は、培養液中の酸素飽和度が７０％以上となるように、循環部４０により循環される培養液の流量に対する酸素供給量と酸素飽和度の関係を予め準備しておき、その関係により使用者が操作パネル１４で設定した量とする。 Note that the amount of oxygen supplied by the oxygen supply unit 60 is such that the oxygen supply amount and the oxygen saturation with respect to the flow rate of the culture solution circulated by the circulation unit 40 are such that the oxygen saturation in the culture solution is 70% or more. Is set in advance, and the amount set by the user on the operation panel 14 is set based on the relationship.
制御部７０は、図示しないＣＰＵ、ＲＯＭ、ＲＡＭ及びＩ／Ｏを備えており、ＲＯＭに格納されたプログラムにより下記（ア）〜（エ）の処理を実行する。
（ア）操作パネル１４の温度設定値を入力する。
（イ）循環部４０により循環される培養液の温度を加熱部５０の温度センサ５４から入力し、その温度値に基づき、加熱部５０のヒータ５２で培養液を加熱し、操作パネル１４から入力した温度設定値（３５〜４５℃）に加熱して保持する。
（ウ）酸素供給部６０により培養液に供給する酸素量を制御する。
（エ）操作パネル１４における５０℃設定ボタンの状況を入力し、５０℃設定がなされている（５０℃設定ボタンが押下されている）場合、培養液を加熱部５０により、５０℃に加熱し、所定時間（５時間）保持する。
The control unit 70 includes a CPU, a ROM, a RAM, and an I / O (not shown), and executes the following processes (a) to (d) by a program stored in the ROM.
(A) The temperature setting value of the operation panel 14 is input.
(A) The temperature of the culture solution circulated by the circulation unit 40 is input from the temperature sensor 54 of the heating unit 50, and the culture solution is heated by the heater 52 of the heating unit 50 based on the temperature value and input from the operation panel 14. Heat and hold at the set temperature (35-45 ° C.).
(C) The oxygen supply unit 60 controls the amount of oxygen supplied to the culture solution.
(D) When the status of the 50 ° C. setting button on the operation panel 14 is input and 50 ° C. is set (when the 50 ° C. setting button is pressed), the culture medium is heated to 50 ° C. by the heating unit 50. , Hold for a predetermined time (5 hours).
泡出しユニット２０は、図１に示すように、前面先端に入水口２１、前面中央に出水口（ジェットノズル）２２、後端に入水用ホース３０を接続するための入水用ホース接続口２３及び出水用ホースを接続するための出水用ホース接続口２４を備えている。 As shown in FIG. 1, the foaming unit 20 has a water inlet 21 at the front end, a water outlet (jet nozzle) 22 at the front center, and a water hose connection 23 for connecting a water hose 30 at the rear end. A water discharge hose connection port 24 for connecting the water discharge hose is provided.
また、先端後面に１個、背面に２個、泡出しユニット２０を浴槽の壁面に固定するための吸盤２５が取り付けられている。
また、泡出しユニット２０には、入水口２１の内部に図示しないフィルタが設けられており、浴槽内の培養液を吸い込む際の不純物を濾過するようになっている。
［バチルス粉末の製作］
真菰、葦、茅、稲藁などイネ科の植物や糠、糖蜜、穀類等の植物由来の材料等を用いてバチルス属細菌を多量に含む発酵粉末を作る事が可能である。特に自然状態の水辺の植物は、バチルス・サブティリス等の有効バチルス芽胞が付着しており材料として適している。酸素温度湿度管理等の発酵コントロールを行い、有効バチルス芽胞を多量（１×１０8ＣＦＵ／ｇ以上）含んだ生成物（バチルス粉末）を作ることができる。
Moreover, the suction cup 25 for fixing the foaming unit 20 to the wall surface of a bathtub is attached to the back surface at the front-end | tip and two on the back.
Further, the foaming unit 20 is provided with a filter (not shown) inside the water inlet 21 so as to filter impurities when the culture solution in the bathtub is sucked.
[Production of Bacillus powder]
Fermented powder containing a large amount of Bacillus bacteria can be made using gramineous plants such as snapper, persimmon, persimmon and rice straw, and plant-derived materials such as persimmon, molasses and cereals. In particular, waterside plants in a natural state are suitable as materials because effective bacillus spores such as Bacillus subtilis are attached. By performing fermentation control such as oxygen temperature and humidity control, a product (Bacillus powder) containing a large amount (1 × 10 8 CFU / g or more) of effective Bacillus spores can be produced.
粉末の粒子サイズは浮遊粒子を増し底への沈殿を少なくするために直径１００μｍ以下が望ましい。
生成物（バチルス粉末）は発酵が二次発酵以上に進みタンパク等の皮膚に抗原となりうる物質や有害物質、有害微生物を低減させておく必要がある。
The particle size of the powder is desirably 100 μm or less in diameter in order to increase suspended particles and reduce sedimentation to the bottom.
The product (Bacillus powder) has to be fermented more than secondary fermentation, and it is necessary to reduce substances such as proteins, which can be antigens to skin, harmful substances, and harmful microorganisms.
真菰は自然に生育しているものも多く栽培が容易で農薬などを使う必要がなく発酵材料として適している。真菰の発酵粉末１００ｇには、たんぱく質：１３〜２０ｇ、脂質：０．８〜１．９ｇ、炭水化物：３４〜５６ｇ、食物繊維：３５〜６５ｇ程が含まれる。
［入浴治療用培養液の製作］
入浴治療用（培養浴水）は、浴水１５０〜１８０Ｌに約６００ｇの真菰発酵粉末などのバチルス粉末を懸濁し、３５〜４５度の温度管理 酸素飽和度７０％以上の酸素供給と攪拌を行い、数日間新たな発酵を生じさせる。条件が満たされると嫌気性の腐敗が生じることなく本来のバチルスの好気的繁殖が生じバチルス粉末内の有機物の代謝分解が行われる。条件が整うと浴水内に１０6〜１０7ＣＦＵ／ｍｌ以上のバチルス属の有効細菌が効率よく培養され皮膚炎の原因菌である黄色ブドウ球菌や酵母様真菌であるマラセチアやカンジダを抑制する。
Mushrooms are naturally grown and are easy to cultivate and do not require the use of pesticides. 100 g of freshly brewed snapper contains protein: 13-20 g, lipid: 0.8-1.9 g, carbohydrate: 34-56 g, dietary fiber: 35-65 g.
[Production of culture solution for bath treatment]
For bathing treatment (culture bath water), about 600 g of Bacillus powder such as sesame seed fermented powder is suspended in 150 to 180 L of bath water, temperature control at 35 to 45 degrees, oxygen supply with oxygen saturation of 70% or more and stirring are performed. Cause a new fermentation for several days. When the conditions are met, the anaerobic growth of the original Bacillus occurs without causing anaerobic decay and the organic matter in the Bacillus powder is metabolized. When the conditions are met, effective bacteria of the genus Bacillus of 10 6 to 10 7 CFU / ml or more are efficiently cultured in the bath water to suppress staphylococcus aureus and yeast-like fungi such as malassezia and candida. .
バチルス属細菌は土壌中に広く生息し枯れた植物を分解する従属栄養細菌であり病原性は低い、納豆菌もその１つである。
バチルス属細菌は、バチルス・サブティリス（Ｂａｃｉｌｌｕｓ ｓｕｂｔｉｌｌｉｓ）、バチルス・メガテリウム（Ｂａｃｉｌｌｕｓ ｍｅｇａｔｅｒｉｕｍ）、バチルス・レンタス（Ｂａｃｉｌｌｕｓ ｌｅｎｔｕｓ）のような、常に酸素を必要とする偏性好気性細菌とバチルス・サーキュランス（Ｂａｃｉｌｌｕｓ ｃｉｒｃｕｌａｎｓ）、バチルス・リケニフォルミス（Ｂａｃｉｌｌｕｓ ｌｉｃｈｅｎｉｆｏｒｍｉｓ）、バチルス・セレウス（Ｂａｃｉｌｌｕｓ ｃｅｒｅｕｓ）のように、酸素が利用できない環境では嫌気性代謝を行う通性好気性細菌が存在する。
Bacillus bacteria are heterotrophic bacteria that inhabit the soil and decompose dead plants, and have low pathogenicity, such as Bacillus natto.
Bacteria belonging to the genus Bacillus subtilis (Bacillus subtilis), Bacillus megaterium (Bacillus megaterium), Bacillus lentus (Bacillis lentus), obligately aerobic bacteria and Bacillus circulans that always require oxygen (Bacillus lentus). There are facultative aerobic bacteria that perform anaerobic metabolism in environments where oxygen is not available, such as Bacillus circulans, Bacillus licheniformis, and Bacillus cereus.
酸素が少ない静水の底付近では、通性好気性細菌や嫌気性菌による嫌気性代謝が行われ人体に有害で悪臭原因ともなる 硫化水素（Ｈ２Ｓ）メタン（ＣＨ４）メルカプタン低級脂肪酸のプロピオン酸やノルマル酪酸などが形成される。これらの悪臭物質は不快感のみならず人体に有害性があり皮膚炎を悪化させる原因となる。 Near the bottom of still water with little oxygen, anaerobic metabolism by facultative aerobic bacteria and anaerobic bacteria is performed, which is harmful to humans and causes odor. Propionic acid and normal of lower hydrogen sulfide (H2S) methane (CH4) mercaptan fatty acid Butyric acid is formed. These malodorous substances are harmful to the human body as well as unpleasant and cause dermatitis to worsen.
浴水の活性が高いバチルス菌数を維持し、入浴剤のバチルス粉末や人体から排泄される脂肪やタンパク質の代謝から産生される有害物質を減らすには、好気的な水質条件を維持する酸素供給（エアレーション）、沈殿しやすい懸濁粒子を常に攪拌し、浴槽低層の嫌気条件を改善し有害物質の揮発を促すための攪拌（サーキュレーション）と２４時間培養温度を安全に維持できる温度管理（ヒーティング）が必要である。 Oxygen that maintains aerobic water quality conditions in order to maintain the number of Bacillus bacteria with high activity in bath water and reduce harmful substances produced from the metabolism of fat and proteins excreted from the bath powder Bacillus and the human body Supply (aeration), constantly stirring suspended particles that are likely to settle, stirring (circulation) to improve anaerobic conditions in the lower layer of the bath and promote the volatilization of harmful substances, and temperature control that can safely maintain the culture temperature for 24 hours ( Heating) is required.
なお、この培養液は皮膚炎の入浴者１名に対して専用に使用する。また、この培養液は1ヵ月後から少量のバチルス粉末を添加し３ヶ月以降に浴水の数分の１だけの水換えを行うだけで維持され有効な皮膚炎の治療が可能である。
［培養液循環装置１の特徴］
（酸素供給及び攪拌を行わない条件下での硫化水素とメルカプタンの発生）
前述のように、バチルス浴水は約１５０〜１８０Ｌの浴水に約６００ｇのバチルス粉末を懸濁させる。そこで、浴水と等濃度、１．５Ｌに６ｇの製造方法が異なった４種類（Ａ，Ｂ，Ｃ，Ｄ）の真菰発酵粉末を懸濁し、３５℃のインキュベーター内で密閉容器内に発生する硫化水素とメルカプタン濃度を、ガス検知管（ＧＡＳＴＥＣ）を使用して測定した結果を表１及び表２に示す。なお、アンモニア ジクロロメタンは検知されなかった。
This culture solution is exclusively used for one dermatitis bather. In addition, this culture solution is maintained only by adding a small amount of Bacillus powder after one month and changing water by a fraction of the bath water after three months, and can effectively treat dermatitis.
[Characteristics of culture medium circulating apparatus 1]
(Hydrogen sulfide and mercaptan generation under conditions without oxygen supply and stirring)
As described above, about 600 g of Bacillus powder is suspended in about 150 to 180 L of bath water. Therefore, 4 kinds (A, B, C, D) of freshly baked cinnamon powder, which have the same concentration as bath water and 6g in 1.5L, are suspended and generated in a sealed container in a 35 ° C incubator. Tables 1 and 2 show the results of measuring hydrogen sulfide and mercaptan concentrations using a gas detector tube (GASTEC). In addition, ammonia dichloromethane was not detected.
（各条件下での硫化水素の発生状況）
前述と同様の濃度の懸濁液を１．５Ｌの密閉が可能な３つの容器に分け、４０℃のインキュベーター内で、Ａ：エアレーション攪拌なし、Ｂ：スターラーによる攪拌と、Ｏ２産生タブレット（過酸化カルシウム）、Ｃ：ポンプによるエアレーションと攪拌をおこなった結果を表３に示す（＊Ｃはエアレーション停止後３時間の密閉状態で測定）。硫化水素の容器内濃度を、ガス検知管（ＧＡＳＴＥＣ）を使用して測定した。
(Generation status of hydrogen sulfide under each condition)
The suspension having the same concentration as described above is divided into three 1.5 L sealable containers. In an incubator at 40 ° C., A: no aeration stirring, B: stirring with a stirrer, O2 production tablet (peroxide Calcium), C: The results of aeration and agitation with a pump are shown in Table 3 (* C is measured in a sealed state for 3 hours after aeration is stopped). The concentration of hydrogen sulfide in the container was measured using a gas detector tube (GASTEC).
Ｂ：７〜１８％、−５０〜０ｍＶ
Ｃ：７０〜９３％、＋１２０〜２２０ｍＶ
表３に示すように、Ａでは酸素供給もなく攪拌もないため容器の底に沈殿層ができ嫌気代謝が行われ、多量の硫化水素が発生している。
B: 7 to 18%, −50 to 0 mV
C: 70 to 93%, +120 to 220 mV
As shown in Table 3, in A, there is no oxygen supply and no agitation, so a precipitate layer is formed at the bottom of the container, anaerobic metabolism is performed, and a large amount of hydrogen sulfide is generated.
Ｂでは攪拌と共に酸素供給（０．７５ｍｇ／Ｌ／ｍｉｎ）が弱いながらもなされバクテリアの好気代謝に使われたため酸素飽和度は上昇しなかったが嫌気代謝による硫化水素の発生はかろうじて防げた。 In B, although oxygen supply (0.75 mg / L / min) was weak with stirring and was used for aerobic metabolism of bacteria, oxygen saturation did not increase, but generation of hydrogen sulfide due to anaerobic metabolism was barely prevented.
Ｃでは酸素供給（１３．６ ｍｇ／Ｌ／ｍｉｎ）と攪拌がなされ好気状態が維持されているため硫化水素は発生しなかった。
また、硫化水素はシステイン等のＳ（イオウ）を含むアミノ酸の嫌気代謝により産生され強い悪臭と腐食性を持つ 実験では酸素供給と攪拌により産生が抑制された。さらに、酸素供給と共に攪拌がされないと底の沈殿層での嫌気代謝が増加する。また、培養液循環装置１を使用する以前は浴水に真菰発酵粉末を懸濁させた後に悪臭が生じ問題になっていた。システムの導入により悪臭の問題は解決した。
（酸素飽和度と嫌気性菌の割合 硫化水素・メルカプタン濃度の関係）
前述の真菰浴水懸濁液Ａ，Ｂ，Ｃを２週間４０℃で培養後、重症アトピー性皮膚炎患者皮膚から浴水に溶出するタンパク量に換算した０．３ｃｃ／Ｌ／ｄａｙの人血漿を培養懸濁液に４日間投与後の嫌気性菌が産生する硫化水素とメルカプタン類の容器内濃度を、ガス検知管（ＧＡＳＴＥＣ）を使用して測定した結果を表４に示す。
In C, oxygen supply (13.6 mg / L / min) and stirring were carried out and the aerobic state was maintained, so hydrogen sulfide was not generated.
Hydrogen sulfide was produced by anaerobic metabolism of amino acids containing S (sulfur) such as cysteine, and in an experiment with strong malodor and corrosivity, production was suppressed by supplying oxygen and stirring. Furthermore, anaerobic metabolism in the bottom sediment layer increases if stirring is not performed with oxygen supply. Moreover, before using the culture-medium circulation apparatus 1, a malodor produced and it became a problem after suspending a true fermented powder in bath water. The introduction of the system solved the problem of bad odor.
(Relationship between oxygen saturation and anaerobic bacteria hydrogen sulfide / mercaptan concentration)
After culturing the amber bath water suspensions A, B and C described above at 40 ° C. for 2 weeks, 0.3 cc / L / day human plasma converted to the amount of protein eluted from the skin of patients with severe atopic dermatitis into the bath water Table 4 shows the results obtained by measuring the concentration of hydrogen sulfide and mercaptans produced by anaerobic bacteria after 4 days in the culture suspension using a gas detector tube (GASTEC).
また、酸素濃度が低い環境下では酸化還元電位は低下し嫌気性菌の発生が多くなりそれとともに硫化水素、メルカプタンの発生が増え、悪臭が発生し水質は悪化し腐敗が生じた。一方 酸素が充分供給された好気状態では嫌気性菌は少数であり嫌気性腐敗産物は産生されなかった。 In the environment where the oxygen concentration is low, the oxidation-reduction potential decreases and the generation of anaerobic bacteria increases. At the same time, the generation of hydrogen sulfide and mercaptans increases, malodors occur, the water quality deteriorates, and rot occurs. On the other hand, in an aerobic state where oxygen was sufficiently supplied, anaerobic bacteria were few and no anaerobic spoilage products were produced.
よってバチルス浴水の安全性を維持するには好気状態を維持できる十分なエアレーション機能が必要であることが分かる。
Ａ：では攪拌・酸素供給がなく代謝が悪く投与されたタンパク成分は分解されないまま残存したためガス発生量はＢより少なくなっていると考えられる気化しない分余計に水質は悪化している。Ｂ：攪拌と共に酸素供給（０．７５ｍｇ／Ｌ／ｍｉｎ）が弱いながらもなされたがバクテリアの酸素消費量をまかない切れず嫌気代謝が増大し多量の硫化水素が発生している。
（培養液循環装置１の酸素供給能力）
１８０Ｌの浴水に６００ｇの真菰発酵粉末を懸濁し１４日経過した４０℃浴水での酸素供給能力について、５Ｌ／ｍｉｎアクアリウム用のポンプと５Ｌ／ｍｉｎの酸素供給部６０で行った比較試験の結果を表５及び図３〜図５に示す。
Therefore, it can be understood that a sufficient aeration function capable of maintaining an aerobic state is necessary to maintain the safety of the Bacillus bath water.
In A: there is no agitation / oxygen supply and the metabolism is poor and the administered protein component remains undegraded, so the gas generation amount is considered to be less than B, and the water quality is deteriorated. B: Although the oxygen supply (0.75 mg / L / min) was weak with stirring, the oxygen consumption of bacteria was not covered, but anaerobic metabolism increased and a large amount of hydrogen sulfide was generated.
(Oxygen supply capacity of the culture medium circulation device 1)
About the oxygen supply capacity in 40 liters bath water after suspending 600g of sesame fermented powder in 180 liters of bath water after 14 days, the comparison test conducted in the pump for 5L / min aquarium and the oxygen supply part 60 of 5L / min. The results are shown in Table 5 and FIGS.
アクアリウム用ポンプでは１８０分後でも酸素飽和度７８％であったのに対して培養液循環装置１では４０分後には酸素は飽和し９９％に達した。
また、図３に示すアクアリウム用ポンプの酸素供給性能と図４に示す培養液循環装置１の循環部４０の酸素供給性能において、１８０Ｌ浴水での両者の酸素供給性能を供給グラフの近似式を比較すると０．３８ｍｇ／Ｌ／ｍｉｎ １．０２ｍｇ／ Ｌ／ｍｉｎであり約２．７倍の違いが見られた。
In the aquarium pump, the oxygen saturation was 78% even after 180 minutes, whereas in the culture medium circulating apparatus 1, the oxygen was saturated and reached 99% after 40 minutes.
Further, in the oxygen supply performance of the aquarium pump shown in FIG. 3 and the oxygen supply performance of the circulation part 40 of the culture medium circulation apparatus 1 shown in FIG. In comparison, it was 0.38 mg / L / min 1.02 mg / L / min, indicating a difference of about 2.7 times.
５％ブドウ糖液０．５ｃｃをバチルス培養液１４．５ｃｃに入れ入れ簡易血糖測定器にて３回血糖測定しバラツキがない事を確認後３ｃｃずつ５つの容器に分注し２５℃、４０℃のインキュベーター内で培養し２４時間後のブドウ糖の消費を指標に活性度を測定した結果を表６に示す。
Place 0.5cc of 5% glucose solution in 14.5cc of Bacillus culture solution, measure blood glucose 3 times with a simple blood glucose meter, confirm that there is no variation, and dispense 3cc into 5 containers at 25 ℃ and 40 ℃. Table 6 shows the results of measuring the activity after culturing in an incubator and using glucose consumption 24 hours later as an index.
今までの民間療法では持続的な温度管理は成されてなかった。
バクテリアは低温になると活性が著しく低下し 表６に見られるように４０℃では２ ４時間のブドウ糖消費は71％であるのに対して２５℃では5％まで低下している。こう した有効細菌であるバチルスの代謝の低下は病原菌の抑制能や老廃物の分解能力の低下 を意味する。ＢＳＴ・ＭＳＴでは適正な温度を維持する管理システムが必要である。
（バチルス属細菌の連続培養）
浴水と同様の濃度１５０ｃｃに０．６ｇ粉末を懸濁し、真菰粉末を連日添加した結果を表７に示す。
Until now, folk remedies have not been able to maintain temperature control.
Bacteria are markedly reduced in activity at low temperatures, as shown in Table 6, glucose consumption for 24 hours is 71% at 40 ° C, while it is reduced to 5% at 25 ° C. A decrease in the metabolism of these effective bacteria, Bacillus, means a decrease in the ability to control pathogens and the ability to decompose waste products. In BST / MST, a management system for maintaining an appropriate temperature is required.
(Continuous culture of Bacillus bacteria)
Table 7 shows the results obtained by suspending 0.6 g powder in 150 cc of the same concentration as the bath water and adding the true powder every day.
菌数は指数表示（ＣＦＵ／ｍｌ）菌数は栄養の消費に伴い低下する。８乗レベルのバチルス菌数を保持するためには１５０ｍｌに対して１０ｍｇ、１５０Ｌの浴水に対して１日１０ｇ程度の発酵粉末の添加が必要である。 The number of bacteria is displayed as an index (CFU / ml). The number of bacteria decreases with consumption of nutrients. In order to maintain the number of Bacillus bacteria at the eighth power level, it is necessary to add 10 mg of fermented powder to 150 ml of bath water and about 10 g per day to 150 L of bath water.
添加をせずに入浴のみを行うとバチルス属細菌は次第に減数しシュードモナス属細菌が優位菌に変わり、皮膚炎の改善能力は失われる。
（５０℃加温処理システム）
培養液循環装置には５０℃加温処理ボタンがあり病原性菌の発育を抑制できる温度処理機能が設けられている。
If only bathing is performed without addition, Bacillus bacteria will gradually decrease, Pseudomonas bacteria will become dominant bacteria, and the ability to improve dermatitis will be lost.
(50 ° C heating system)
The culture fluid circulation device has a 50 ° C. heating processing button and is provided with a temperature processing function capable of suppressing the growth of pathogenic bacteria.
細菌は至適発育温度によって、１０〜２０℃：低温性菌、３０〜４０℃：中温性菌、５０〜６０℃：高温性菌の３つのグループに分類される。病原制菌のほとんどが中温性菌に含まれる。一方、土壌菌であるバチルス属細菌は高温でも増殖が可能で芽胞形成能があり一旦芽胞を形成すると１００℃でも不活化できない。 Bacteria are classified into three groups according to the optimal growth temperature: 10-20 ° C .: psychrophilic bacteria, 30-40 ° C .: mesophilic bacteria, 50-60 ° C .: thermophilic bacteria. Most pathogenic bacteria are contained in mesophilic bacteria. On the other hand, Bacillus bacteria, which are soil bacteria, can grow at high temperatures and have spore-forming ability, and once formed, they cannot be inactivated even at 100 ° C.
５０℃加温処理は自動的に５０℃を５時間維持した後処理を終了する夜間入浴後にボタンを押すと翌日午前までに処理が終了し入浴温度にする。５０℃という温度は人が間違って接触しても軽微な低温熱傷で済み安全性も確保できる。
（５０℃処理の効果検証結果）
黄色ブドウ球菌、カンジダ、浴水中バチルスを一般ブイヨン３６℃で攪拌培養し菌数を測定後５０℃で５時間の高温処理し直後に菌数を測定し、３６℃で培養したものを対照とした結果を表８に示す。なお、レジオネラニューモフィリアはＢＣＹＥαブイヨンにて培養した 。（ＣＦＵ／ｍｌ）
The 50 ° C. heating treatment automatically maintains the temperature at 50 ° C. for 5 hours and then terminates the treatment. When the button is pressed after bathing at night, the treatment is finished by the morning of the following day and the bath temperature is reached. A temperature of 50 ° C. can ensure a safe operation even if a person accidentally touches it, and only requires a low temperature burn.
(Results of verifying the effect of 50 ℃ treatment)
Staphylococcus aureus, Candida, and Bacillus in bath water were stirred and cultured at 36 ° C in a general bouillon, followed by high-temperature treatment at 50 ° C for 5 hours. The results are shown in Table 8. Legionella pneumophilia was cultured in BCYEα broth. (CFU / ml)
１… 培養液循環装置 １０… 本体 １１… 筐体 １２… 蓋 １４… 操作パネル ２０… 泡出しユニット ２１… 入水口 ２２… 出水口（ジェットノズル） ２３… 入水用ホース接続口 ２４… 出水用ホース接続口 ２５… 吸盤 ３０… 入水用ホース ３２… 出水用ホース ４０… 循環部 ５０… 加熱部 ５２… ヒータ ５４… 温度センサ ６０… 酸素供給部 ７０… 制御部。 DESCRIPTION OF SYMBOLS 1 ... Culture-medium circulation apparatus 10 ... Main body 11 ... Case 12 ... Cover 14 ... Operation panel 20 ... Foaming unit 21 ... Water inlet 22 ... Water outlet (jet nozzle) 23 ... Water inlet hose connection port 24 ... Water outlet hose connection Mouth 25 ... Suction cup 30 ... Hose for water entry 32 ... Hose for water discharge 40 ... Circulation part 50 ... Heating part 52 ... Heater 54 ... Temperature sensor 60 ... Oxygen supply part 70 ... Control part.
Claims (2)
該バチルス粉末を懸濁した浴水を３５〜４５℃の温度に保つとともに、酸素飽和度７０％以上の酸素供給を行って発酵させることにより得られることを特徴とする入浴治療用の培養液。 600 g of Bacillus powder is suspended in 150 to 180 liters of bath water,
A culture solution for bathing treatment, which is obtained by maintaining the bath water in which the Bacillus powder is suspended at a temperature of 35 to 45 ° C. and fermenting by supplying oxygen with an oxygen saturation of 70% or more.
前記循環手段により循環される前記培養液を加熱する加熱手段と、
前記循環手段により循環される前記培養液中に酸素を供給する酸素供給手段と、
前記循環手段により循環される前記培養液の温度を前記加熱手段により３５〜４５℃に加熱して保持させるとともに、前記循環手段で循環される前記培養液の温度を前記加熱手段により５０℃に加熱して所定時間保持し、さらに、前記酸素供給手段により前記培養液の酸素飽和度を７０％以上にする制御手段と、
を備えたことを特徴とする入浴治療用の培養液循環装置。 Circulation means for circulating the culture solution according to claim 1 placed in a bathtub;
Heating means for heating the culture medium circulated by the circulation means;
Oxygen supply means for supplying oxygen into the culture medium circulated by the circulation means;
The temperature of the culture medium circulated by the circulation means is heated and maintained at 35 to 45 ° C. by the heating means, and the temperature of the culture liquid circulated by the circulation means is heated to 50 ° C. by the heating means. And a control means for maintaining the oxygen saturation of the culture broth by 70% or more by the oxygen supply means,
A culture medium circulating apparatus for bathing treatment, comprising:
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2013190646A JP6230349B2 (en) | 2013-09-13 | 2013-09-13 | Culture solution for bathing treatment and circulation device for culture solution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2013190646A JP6230349B2 (en) | 2013-09-13 | 2013-09-13 | Culture solution for bathing treatment and circulation device for culture solution |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2015053923A JP2015053923A (en) | 2015-03-23 |
| JP6230349B2 true JP6230349B2 (en) | 2017-11-15 |
Family
ID=52818765
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013190646A Active JP6230349B2 (en) | 2013-09-13 | 2013-09-13 | Culture solution for bathing treatment and circulation device for culture solution |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP6230349B2 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2023167519A (en) * | 2022-05-12 | 2023-11-24 | 株式会社ネイチャーサポート | Liquid culture medium for improving immunity and promoting normal immune formation, and liquid culture medium generator |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2833247B2 (en) * | 1991-03-26 | 1998-12-09 | 松下電器産業株式会社 | Bath water purification and warming device |
| JPH10249388A (en) * | 1997-03-13 | 1998-09-22 | Washi Kosan Kk | Bath using antimicrobial microorganism |
| AU749001B2 (en) * | 1997-04-18 | 2002-06-13 | Ganeden Biotech, Inc. | Topical use of probiotic bacillus spores to prevent or control microbial infections |
| JP2005230041A (en) * | 2004-02-17 | 2005-09-02 | Matsushita Electric Ind Co Ltd | Bathtub air bubble jetting apparatus |
| JP2006102304A (en) * | 2004-10-07 | 2006-04-20 | Matsushita Electric Ind Co Ltd | Oxygen enrichment warm-water supplying apparatus |
| JP4875434B2 (en) * | 2006-08-11 | 2012-02-15 | 大阪瓦斯株式会社 | Bath equipment |
| JP2008149307A (en) * | 2006-12-18 | 2008-07-03 | Seiko Enterprise:Kk | 24-hour bathing system |
-
2013
- 2013-09-13 JP JP2013190646A patent/JP6230349B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| JP2015053923A (en) | 2015-03-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2018522878A (en) | Microbiome compatible cosmetics | |
| CN105176925B (en) | A kind of immunocyte serum free medium and application thereof | |
| CN107982570A (en) | One kind eliminates the unusual smell conditioning liquid, its preparation method and the sanitary napkin that eliminates the unusual smell | |
| CN104649816A (en) | Culture medium special for agrocybe cylindracea | |
| JP6230349B2 (en) | Culture solution for bathing treatment and circulation device for culture solution | |
| JP5548931B1 (en) | Earthworm dry powder manufacturing method | |
| TW200822860A (en) | Embalming mixture, preparation method therefor, and method of treating corpses with the embalming mixture | |
| JP5654666B2 (en) | Method for producing fermented natural products | |
| CN206621479U (en) | Supply the skin-friendly absorbing structure of oxygen | |
| CN109762723B (en) | Device for photosynthetic bacteria culture | |
| JP2001129580A (en) | Apparatus for treating waste water containing oil and fat | |
| CN107648674A (en) | With antibacterial and the metal implant for promoting Integrated implant function and preparation method thereof | |
| CN109089466A (en) | A method of promoting celery germination | |
| CN206275888U (en) | A kind of particulate suction-type Chinese medicine health-care medical treatment device | |
| CN209957772U (en) | A device for photosynthetic bacteria culture | |
| CN101380263A (en) | Biological internal environment conditioning device and use method thereof | |
| CN106986517A (en) | A kind of cultivation substrate modifier and preparation method thereof | |
| CN107950303A (en) | A kind of cultural method of Termitomyces albuminosus with black skin parent species | |
| Vijayan et al. | Biocontrol of fungal diseases in large cardamom using Pseudomonas fluorescens | |
| CN208684544U (en) | A kind of microorganism continuous culture tank administered for black and odorous water | |
| CN106550763A (en) | A kind of artificial culture method of Periostracum cicadae spore powder | |
| CN109717139A (en) | The nonreactive cultivation method of pig | |
| CN110305812A (en) | A kind of the lichen bacillus ferments culture process | |
| CN105199961B (en) | The sharp top Fusariumsp of one kind and its application | |
| CN108040888A (en) | A kind of livestock and poultry cultivation active compound probiotic nutrition ferment and preparation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20160905 |
|
| A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20170720 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20170725 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20170922 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20171010 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20171017 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 6230349 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |