JP6226888B2 - ポリマーの測定の解析 - Google Patents

ポリマーの測定の解析 Download PDF

Info

Publication number
JP6226888B2
JP6226888B2 JP2014557122A JP2014557122A JP6226888B2 JP 6226888 B2 JP6226888 B2 JP 6226888B2 JP 2014557122 A JP2014557122 A JP 2014557122A JP 2014557122 A JP2014557122 A JP 2014557122A JP 6226888 B2 JP6226888 B2 JP 6226888B2
Authority
JP
Japan
Prior art keywords
feature vector
polymer
measurements
derived
similarity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2014557122A
Other languages
English (en)
Japanese (ja)
Other versions
JP2015509710A (ja
JP2015509710A5 (https=
Inventor
ウィリアム レイド,スチュアート
ウィリアム レイド,スチュアート
アンソニー クラーク,ジェームス
アンソニー クラーク,ジェームス
ホワイト,ジェームス
ハーパー,ギャヴィン
Original Assignee
オックスフォード ナノポール テクノロジーズ リミテッド
オックスフォード ナノポール テクノロジーズ リミテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by オックスフォード ナノポール テクノロジーズ リミテッド, オックスフォード ナノポール テクノロジーズ リミテッド filed Critical オックスフォード ナノポール テクノロジーズ リミテッド
Publication of JP2015509710A publication Critical patent/JP2015509710A/ja
Publication of JP2015509710A5 publication Critical patent/JP2015509710A5/ja
Application granted granted Critical
Publication of JP6226888B2 publication Critical patent/JP6226888B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • G01N33/487Physical analysis of biological material of liquid biological material
    • G01N33/48785Electrical and electronic details of measuring devices for physical analysis of liquid biological material not specific to a particular test method, e.g. user interface or power supply
    • G01N33/48792Data management, e.g. communication with processing unit
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6858Allele-specific amplification
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B82NANOTECHNOLOGY
    • B82YSPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/02Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • G01N33/487Physical analysis of biological material of liquid biological material
    • G01N33/48707Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721Investigating individual macromolecules, e.g. by translocation through nanopores
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6875Nucleoproteins
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10Sequence alignment; Homology search
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00ICT specially adapted for sequence analysis involving nucleotides or amino acids

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Nanotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Medical Informatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Theoretical Computer Science (AREA)
  • Evolutionary Biology (AREA)
  • Cell Biology (AREA)
  • Human Computer Interaction (AREA)
  • Databases & Information Systems (AREA)
  • Optics & Photonics (AREA)
  • Electrochemistry (AREA)
JP2014557122A 2012-02-16 2013-02-18 ポリマーの測定の解析 Active JP6226888B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261599573P 2012-02-16 2012-02-16
US61/599,573 2012-02-16
PCT/GB2013/050381 WO2013121224A1 (en) 2012-02-16 2013-02-18 Analysis of measurements of a polymer

Publications (3)

Publication Number Publication Date
JP2015509710A JP2015509710A (ja) 2015-04-02
JP2015509710A5 JP2015509710A5 (https=) 2017-08-31
JP6226888B2 true JP6226888B2 (ja) 2017-11-08

Family

ID=47749886

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2014557122A Active JP6226888B2 (ja) 2012-02-16 2013-02-18 ポリマーの測定の解析

Country Status (10)

Country Link
US (3) US20150057948A1 (https=)
EP (2) EP3736339B1 (https=)
JP (1) JP6226888B2 (https=)
KR (1) KR102106499B1 (https=)
CN (1) CN104321441B (https=)
AU (1) AU2013220179B2 (https=)
BR (1) BR112014020211A2 (https=)
CA (1) CA2866587C (https=)
IN (1) IN2014DN06795A (https=)
WO (1) WO2013121224A1 (https=)

Families Citing this family (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
US8285719B1 (en) 2008-08-08 2012-10-09 The Research Foundation Of State University Of New York System and method for probabilistic relational clustering
EP3663412B1 (en) * 2011-09-23 2022-03-09 Oxford Nanopore Technologies PLC Analysis of a polymer comprising polymer units by means of translocation through a nanopore
GB201202519D0 (en) 2012-02-13 2012-03-28 Oxford Nanopore Tech Ltd Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules
BR112014020211A2 (pt) 2012-02-16 2017-07-04 Oxford Nanopore Tech Ltd métodos para analisar uma série ordenada por tempo de medições de um polímero, para estimar a presença, ausência ou quantidade de um polímero alvo, e para determinar uma alteração em um polímero, programa de computador, e, dispositivos de análise e de diagnóstico
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
GB201222928D0 (en) 2012-12-19 2013-01-30 Oxford Nanopore Tech Ltd Analysis of a polynucleotide
EP3971300B1 (en) 2014-10-16 2024-06-05 Oxford Nanopore Technologies plc Sorting of polymers
GB201418512D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Electrical device with detachable components
US10302972B2 (en) 2015-01-23 2019-05-28 Pacific Biosciences Of California, Inc. Waveguide transmission
US10794895B2 (en) 2015-02-05 2020-10-06 President And Fellows Of Harvard College Nanopore sensor including fluidic passage
GB201508669D0 (en) 2015-05-20 2015-07-01 Oxford Nanopore Tech Ltd Methods and apparatus for forming apertures in a solid state membrane using dielectric breakdown
EP3332033B1 (en) 2015-08-06 2021-04-21 Pacific Biosciences of California, Inc. Single-molecule nanofet sequencing systems and methods
WO2017082875A1 (en) 2015-11-10 2017-05-18 Hewlett Packard Enterprise Development Lp Data allocation based on secure information retrieval
AU2017225374B2 (en) 2016-03-02 2020-03-05 Oxford Nanopore Technologies Limited Mutant pore
US11514289B1 (en) 2016-03-09 2022-11-29 Freenome Holdings, Inc. Generating machine learning models using genetic data
US10823721B2 (en) 2016-07-05 2020-11-03 Quantapore, Inc. Optically based nanopore sequencing
KR102610098B1 (ko) * 2016-07-06 2023-12-04 가던트 헬쓰, 인크. 무세포 핵산의 프래그멘톰 프로파일링을 위한 방법
GB201611770D0 (en) 2016-07-06 2016-08-17 Oxford Nanopore Tech Microfluidic device
WO2018034745A1 (en) * 2016-08-18 2018-02-22 The Regents Of The University Of California Nanopore sequencing base calling
US11080301B2 (en) 2016-09-28 2021-08-03 Hewlett Packard Enterprise Development Lp Storage allocation based on secure data comparisons via multiple intermediaries
GB201619930D0 (en) 2016-11-24 2017-01-11 Oxford Nanopore Tech Apparatus and methods for controlling insertion of a membrane channel into a membrane
CN110178012B (zh) * 2016-12-16 2022-04-05 艾珀尔有限公司 分类分析方法、分类分析装置及分类分析用记录介质
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
GB201707138D0 (en) * 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Machine learning analysis of nanopore measurements
JP2020530257A (ja) * 2017-09-15 2020-10-22 イルミナ インコーポレイテッド 配列検出システム
GB2568895B (en) 2017-11-29 2021-10-27 Oxford Nanopore Tech Ltd Microfluidic device
GB2569630B (en) 2017-12-21 2022-10-12 Sharp Life Science Eu Ltd Droplet Interfaces in Electro-wetting Devices
AU2018353924A1 (en) 2017-12-29 2019-07-18 Clear Labs, Inc. Automated priming and library loading device
US12475812B2 (en) 2018-02-02 2025-11-18 Bayer Aktiengesellschaft Control of resistent harmful organisms
WO2019204720A1 (en) * 2018-04-20 2019-10-24 The Regents Of The University Of California Nucleic acid sequencing methods and computer-readable media for practicing same
GB2574048B (en) 2018-05-24 2021-06-16 Oxford Nanopore Tech Ltd Nanopore sensor component with electrostatic discharge protection
WO2019227013A1 (en) 2018-05-24 2019-11-28 Oxford Nanopore Technologies Inc. Droplet interfaces in electro-wetting devices
GB201814369D0 (en) 2018-09-04 2018-10-17 Oxford Nanopore Tech Ltd Method for determining a polymersequence
US12256863B2 (en) 2018-11-28 2025-03-25 Oxford Nanopore Technologies Plc Sensing system and method of operation
GB201819378D0 (en) * 2018-11-28 2019-01-09 Oxford Nanopore Tech Ltd Analysis of nanopore signal using a machine-learning technique
US11789006B2 (en) 2019-03-12 2023-10-17 Oxford Nanopore Technologies Plc Nanopore sensing device, components and method of operation
WO2020236995A1 (en) * 2019-05-22 2020-11-26 Icahn School Of Medicine At Mount Sinai Method for de novo detection, identification and fine mapping of multiple forms of nucleic acid modifications
CN110628597B (zh) * 2019-10-19 2023-04-25 上海新微技术研发中心有限公司 拉曼光谱法生物分子测序方法
US11651281B2 (en) * 2020-05-18 2023-05-16 International Business Machines Corporation Feature catalog enhancement through automated feature correlation
WO2022013551A1 (en) 2020-07-17 2022-01-20 Oxford Nanopore Technologies Limited Nanopore sensing device
CN113030548A (zh) * 2021-03-09 2021-06-25 南京邮电大学 基于局域阈值的纳米孔检测电流信号特征信息提取方法及装置
GB202103605D0 (en) * 2021-03-16 2021-04-28 Oxford Nanopore Tech Ltd Alignment of target and reference sequences of polymer units
WO2022243692A1 (en) 2021-05-19 2022-11-24 Oxford Nanopore Technologies Plc Methods for complement strand sequencing
WO2023029044A1 (zh) * 2021-09-06 2023-03-09 百图生科(北京)智能技术有限公司 用于单细胞测序的方法、装置、设备、介质和程序产品
CN113837036B (zh) * 2021-09-09 2024-08-02 成都齐碳科技有限公司 生物聚合物的表征方法、装置、设备及计算机存储介质
CN114908358B8 (zh) * 2022-04-14 2024-06-21 孔确(成都)科技有限公司 一种两亲性分子层的制备方法及装置
GB202215442D0 (en) 2022-10-19 2022-11-30 Oxford Nanopore Tech Plc Analysis of a polymer
CN120457488A (zh) 2022-11-10 2025-08-08 拜耳公司 基于遗传谱的靶向性作物保护产品施用
GB202216905D0 (en) 2022-11-11 2022-12-28 Oxford Nanopore Tech Plc Novel pore monomers and pores
JP7630538B2 (ja) * 2023-03-15 2025-02-17 Dic株式会社 樹脂物性値予測装置及び樹脂物性値の予測方法
GB202305524D0 (en) 2023-04-14 2023-05-31 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202307141D0 (en) 2023-05-12 2023-06-28 Oxford Nanopore Tech Plc Modified helicases

Family Cites Families (78)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5795782A (en) * 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US6128587A (en) * 1997-01-14 2000-10-03 The Regents Of The University Of California Method and apparatus using Bayesian subfamily identification for sequence analysis
JPH11178575A (ja) * 1997-12-22 1999-07-06 Hitachi Ltd Dna塩基配列解析装置、方法及び記録媒体
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
WO2000039333A1 (en) 1998-12-23 2000-07-06 Jones Elizabeth Louise Sequencing method using magnifying tags
JP2003502166A (ja) 1999-06-22 2003-01-21 プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ 固体状態の次元的特徴の制御
AU2002239284A1 (en) 2000-11-27 2002-06-03 The Regents Of The University Of California Methods and devices for characterizing duplex nucleic acid molecules
US20020197618A1 (en) 2001-01-20 2002-12-26 Sampson Jeffrey R. Synthesis and amplification of unstructured nucleic acids for rapid sequencing
JP2002325581A (ja) * 2001-04-27 2002-11-12 Adgene Co Ltd 核酸溶解曲線及び核酸解離曲線を用いた未知あるいは既知核酸変異検出法及び表示法
JP2003256435A (ja) 2002-03-06 2003-09-12 Fujitsu Ltd 配列データ統合処理方法、配列データ統合処理装置及び配列データ統合処理プログラム
US7220405B2 (en) 2003-09-08 2007-05-22 E. I. Du Pont De Nemours And Company Peptide-based conditioners and colorants for hair, skin, and nails
US20050136408A1 (en) 2003-12-19 2005-06-23 May Tom-Moy Methods and systems for characterizing a polymer
JP2005176730A (ja) 2003-12-19 2005-07-07 Hitachi Ltd cDNA配列をゲノム配列にマッピングする方法
US7279337B2 (en) 2004-03-10 2007-10-09 Agilent Technologies, Inc. Method and apparatus for sequencing polymers through tunneling conductance variation detection
US7238485B2 (en) 2004-03-23 2007-07-03 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
KR100614827B1 (ko) 2004-05-06 2006-08-25 재단법인서울대학교산학협력재단 양방향 은닉 마코프 모델을 이용한 완숙한마이크로알엔에이 위치예측방법 및 이를 구현하기 위한컴퓨터 프로그램을 기록한 저장매체
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
US20060019259A1 (en) 2004-07-22 2006-01-26 Joyce Timothy H Characterization of biopolymers by resonance tunneling and fluorescence quenching
US20130071837A1 (en) 2004-10-06 2013-03-21 Stephen N. Winters-Hilt Method and System for Characterizing or Identifying Molecules and Molecular Mixtures
US20060086626A1 (en) 2004-10-22 2006-04-27 Joyce Timothy H Nanostructure resonant tunneling with a gate voltage source
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
WO2007065025A2 (en) 2005-11-29 2007-06-07 Wisconsin Alumni Research Foundation Method of dna analysis using micro/nanochannel
WO2007117832A2 (en) 2006-03-12 2007-10-18 Applera Corporation Methods of detecting target nucleic acids
US8116988B2 (en) 2006-05-19 2012-02-14 The University Of Chicago Method for indexing nucleic acid sequences for computer based searching
US7731826B2 (en) 2006-08-17 2010-06-08 Electronic Bio Sciences, Llc Controlled translocation of a polymer in an electrolytic sensing system
US8003319B2 (en) 2007-02-02 2011-08-23 International Business Machines Corporation Systems and methods for controlling position of charged polymer inside nanopore
WO2008102120A1 (en) 2007-02-20 2008-08-28 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
AU2008236694B2 (en) 2007-04-04 2014-01-23 The Regents Of The University Of California Compositions, devices, systems, and methods for using a nanopore
US8698481B2 (en) 2007-09-12 2014-04-15 President And Fellows Of Harvard College High-resolution molecular sensor
GB2453377A (en) * 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
US8628940B2 (en) 2008-09-24 2014-01-14 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
WO2010004265A1 (en) 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Enzyme-pore constructs
JP2011527191A (ja) * 2008-07-07 2011-10-27 オックスフォード ナノポア テクノロジーズ リミテッド 塩基検出細孔
CA3092369C (en) 2008-09-22 2025-09-23 University Of Washington MSP NANOPORES AND ASSOCIATED PROCESSES
CN102257162A (zh) 2008-10-29 2011-11-23 波士顿大学理事会 保持序列的dna转化
US8452546B1 (en) 2008-11-07 2013-05-28 Electronic Biosciences, Inc. Method for deducing a polymer sequence from a nominal base-by-base measurement
GB0820927D0 (en) * 2008-11-14 2008-12-24 Isis Innovation Method
US20120100530A1 (en) 2009-01-30 2012-04-26 Oxford Nanopore Technologies Limited Enzyme mutant
JP5843614B2 (ja) 2009-01-30 2016-01-13 オックスフォード ナノポア テクノロジーズ リミテッド 膜貫通配列決定における核酸構築物のためのアダプター
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
US8986928B2 (en) 2009-04-10 2015-03-24 Pacific Biosciences Of California, Inc. Nanopore sequencing devices and methods
BRPI1012752B1 (pt) 2009-04-20 2019-06-25 Oxford Nanopore Technologies Limited Método e aparelho para detectar uma interação de uma entidade molecular com uma proteína de membrana em uma camada de moléculas anfifílicas
EP3196645B1 (en) 2009-09-18 2019-06-19 President and Fellows of Harvard College Bare single-layer graphene membrane having a nanopore enabling high-sensitivity molecular detection and analysis
CN102741430B (zh) 2009-12-01 2016-07-13 牛津楠路珀尔科技有限公司 生化分析仪器、用于进行生化分析的第一模块以及相关方法
US8748091B2 (en) 2009-12-18 2014-06-10 The Board Of Trustees Of The University Of Illinois Characterizing stretched polynucleotides in a synthetic nanopassage
US8324914B2 (en) 2010-02-08 2012-12-04 Genia Technologies, Inc. Systems and methods for characterizing a molecule
CA3116307C (en) 2010-02-23 2023-10-17 University Of Washington Artificial mycolic acid membranes
US8828211B2 (en) 2010-06-08 2014-09-09 President And Fellows Of Harvard College Nanopore device with graphene supported artificial lipid membrane
EP2614156B1 (en) 2010-09-07 2018-08-01 The Regents of The University of California Control of dna movement in a nanopore at one nucleotide precision by a processive enzyme
US9068221B2 (en) 2011-02-09 2015-06-30 Life Technologies Corporation Method of analysis of genetic markers
AU2012215135B9 (en) 2011-02-11 2017-03-09 Oxford Nanopore Technologies Limited Mutant pores
WO2012135658A2 (en) 2011-03-30 2012-10-04 Noblegen Biosciences, Inc. Sequence preserved dna conversion for optical nanopore sequencing
CN103842519B (zh) 2011-04-04 2018-02-06 哈佛大学校长及研究员协会 通过局部电位测量进行的纳米孔感测
SG10201604316WA (en) 2011-05-27 2016-07-28 Oxford Nanopore Tech Ltd Coupling method
US9145623B2 (en) 2011-07-20 2015-09-29 Thermo Fisher Scientific Oy Transposon nucleic acids comprising a calibration sequence for DNA sequencing
AU2012288629B2 (en) 2011-07-25 2017-02-02 Oxford Nanopore Technologies Limited Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
EP3663412B1 (en) 2011-09-23 2022-03-09 Oxford Nanopore Technologies PLC Analysis of a polymer comprising polymer units by means of translocation through a nanopore
JP6226869B2 (ja) 2011-10-21 2017-11-08 オックスフォード ナノポール テクノロジーズ リミテッド 酵素法
US9617591B2 (en) 2011-12-29 2017-04-11 Oxford Nanopore Technologies Ltd. Method for characterising a polynucleotide by using a XPD helicase
BR112014016112B8 (pt) 2011-12-29 2022-12-27 Oxford Nanopore Tech Ltd Método e aparelho para caracterizar um polinucleotídeo alvo, kit, e, uso de uma helicase
JP6333179B2 (ja) 2012-01-20 2018-05-30 ジニア テクノロジーズ, インコーポレイテッド ナノポアベースの分子検出および配列決定
BR112014020211A2 (pt) 2012-02-16 2017-07-04 Oxford Nanopore Tech Ltd métodos para analisar uma série ordenada por tempo de medições de um polímero, para estimar a presença, ausência ou quantidade de um polímero alvo, e para determinar uma alteração em um polímero, programa de computador, e, dispositivos de análise e de diagnóstico
CA2864824C (en) 2012-02-16 2020-06-16 The Regents Of The University Of California Nanopore sensor for enzyme-mediated protein translocation
JP6271505B2 (ja) 2012-04-10 2018-01-31 オックスフォード ナノポール テクノロジーズ リミテッド 変異体ライセニンポア
WO2013159042A1 (en) 2012-04-19 2013-10-24 University Of Washington Through Its Center For Commercialization Methods and compositions for generating reference maps for nanopore-based polymer analysis
US9116118B2 (en) 2012-06-08 2015-08-25 Pacific Biosciences Of California, Inc. Modified base detection with nanopore sequencing
EP2875128B8 (en) 2012-07-19 2020-06-24 Oxford Nanopore Technologies Limited Modified helicases
US11155860B2 (en) 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
US9797009B2 (en) 2012-07-19 2017-10-24 Oxford Nanopore Technologies Limited Enzyme construct
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
EP2911771B1 (en) 2012-10-26 2017-01-18 Oxford Nanopore Technologies Limited Droplet interfaces
GB201222928D0 (en) 2012-12-19 2013-01-30 Oxford Nanopore Tech Ltd Analysis of a polynucleotide
EP3556869B1 (en) 2013-11-26 2023-08-02 Illumina Inc. Compositions and methods for polynucleotide sequencing
US20170096703A1 (en) 2014-03-21 2017-04-06 Oxford Nanopore Technologies Ltd. Analysis of a polymer from multi-dimensional measurements
GB201408652D0 (en) 2014-05-15 2014-07-02 Oxford Nanopore Tech Ltd Model adjustment during analysis of a polymer from nanopore measurements
US9925679B2 (en) 2014-05-19 2018-03-27 I+D+M Creative, Llc Devices and methods for assisting with slicing items
EP3971300B1 (en) 2014-10-16 2024-06-05 Oxford Nanopore Technologies plc Sorting of polymers

Also Published As

Publication number Publication date
US20190154655A1 (en) 2019-05-23
AU2013220179A1 (en) 2014-09-04
EP2814980B1 (en) 2020-04-15
AU2013220179B2 (en) 2018-11-08
US20150057948A1 (en) 2015-02-26
CN104321441A (zh) 2015-01-28
WO2013121224A1 (en) 2013-08-22
US20240264143A1 (en) 2024-08-08
JP2015509710A (ja) 2015-04-02
CN104321441B (zh) 2016-10-19
CA2866587C (en) 2022-07-26
EP2814980B8 (en) 2020-06-10
KR102106499B1 (ko) 2020-05-04
EP2814980A1 (en) 2014-12-24
BR112014020211A2 (pt) 2017-07-04
KR20140125874A (ko) 2014-10-29
IN2014DN06795A (https=) 2015-05-22
EP3736339A1 (en) 2020-11-11
EP3736339B1 (en) 2022-07-27
US11959906B2 (en) 2024-04-16
CA2866587A1 (en) 2013-08-22

Similar Documents

Publication Publication Date Title
US20240264143A1 (en) Analysis of measurements of a polymer
US12486534B2 (en) Analysis of a polynucleotide via a nanopore system
US12545955B2 (en) Analysis of a polymer
US20230039783A1 (en) Protein and Peptide Fingerprinting and Sequencing by Nanopore Translocation of Peptide-Oligonucleotide Complexes
US20250006308A1 (en) Nanopore measurement signal analysis
Branton et al. The development of nanopore sequencing
WO2025238370A1 (en) Enhanced polynucleotide sequencing

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160212

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20160212

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20170124

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20170421

RD03 Notification of appointment of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7423

Effective date: 20170713

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170724

A524 Written submission of copy of amendment under article 19 pct

Free format text: JAPANESE INTERMEDIATE CODE: A524

Effective date: 20170724

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20170912

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20171010

R150 Certificate of patent or registration of utility model

Ref document number: 6226888

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250