JP6176710B2 - Microbial degradation promoter for organic waste and method for decomposing organic waste using the same - Google Patents

Description

  The present invention eliminates microbial degradation of organic waste such as woody waste such as fallen leaves and pruned branches, agricultural waste such as dead grass and spoiled crops, livestock manure, household garbage, and sludge from water and sewage systems. The present invention relates to a microbial degradation accelerator to be promoted, a method for producing a degradation product of organic waste using the microbial degradation promoter, and a method for decomposing organic waste using the microbial degradation promoter.

  In recent years, due to the progress of urbanization, agriculture, and larger livestock, woody waste such as fallen leaves and pruned branches, agricultural waste such as dead grass and spoiled crops, livestock manure, domestic garbage, sludge from water and sewers A large amount of organic waste such as is generated locally. Most of these organic wastes are disposed of by landfill or incineration. However, the landfill process has problems such as a shortage of landfill. Incineration treatment cannot be easily incinerated by the Dioxin Countermeasures Law, and household garbage, for example, has a problem that it is difficult to burn because of its high moisture and salt content.

  Under such circumstances, from the viewpoint of environmental protection and effective use of resources, a method of composting organic waste using an ancient decomposition method using microorganisms has attracted attention. However, in order to completely compost organic waste using microorganisms, it usually requires a long period of 6 months to 1 year or more, so a large-scale treatment facility is required.

  In addition, organic waste may contain harmful substances such as harmful plant species (such as foreign plant species), antibiotics, and agricultural chemicals. For example, so-called raw compost imported from abroad may contain foreign plant seeds. Therefore, when such raw compost is used, there is a risk that foreign plants will grow in the field. In order to decompose such harmful substances that can be contained in the compost, it is generally preferable to perform an exothermic treatment that ferments at 55 ° C. or more for 3 days or more in the decomposition process by microorganisms. For example, the US Environmental Protection Agency (EPA) mandates that such heat treatment be performed during the compost production process.

  Conventionally, as a method of decomposing and composting organic waste, specific microorganisms, enzymes, nutrients of microorganisms, etc. are added to organic waste to promote the decomposition (composting) of organic waste. A method is known (see, for example, Patent Documents 1 to 6). However, even in these methods, the organic waste decomposition promoting effect is not sufficient.

  Against the background of such conventional technology, development of a microbial degradation promoter capable of promoting microbial degradation of organic waste has been eagerly desired.

JP 2001-261474 A Japanese Patent Laid-Open No. 2003-9848 JP 2007-209308 A JP 2008-245629 A JP 2001-270793 A JP 2003-183090 A

  The present invention eliminates microbial degradation of organic waste such as woody waste such as fallen leaves and pruned branches, agricultural waste such as dead grass and spoiled crops, livestock manure, household garbage, and sludge from water and sewage systems. The main object is to provide a method for promoting microbial degradation promoting, a method for producing a degradation product of organic waste using the microbial degradation promoter, and a method for decomposing organic waste using the microbial degradation promoter. And

The present inventor has intensively studied to solve the above problems, and as a result, the following general formula (1)
It has been found that the decomposition of organic waste by microorganisms is promoted by using a microbial decomposition accelerator for organic waste containing the compound represented by formula (1) as an active ingredient. The present invention has been completed by further studies based on such knowledge.
[In General Formula (1), R ¹ and R ² are the same or different and each represents a linear or branched alkyl group having 4 to 6 carbon atoms, and R ³ is a linear chain having 1 to 8 carbon atoms. Alternatively, it represents a branched alkyl group, and n represents an integer of 1 or more. ]

That is, this invention provides the invention of the aspect hung up below.
Item 1. The following general formula (1):
[In General Formula (1), R ¹ and R ² are the same or different and each represents a linear or branched alkyl group having 4 to 6 carbon atoms, and R ³ is a linear chain having 1 to 8 carbon atoms. Alternatively, it represents a branched alkyl group, and n represents an integer of 1 or more. ]
An organic waste microbial degradation promoter comprising a compound represented by the formula:
Item 2. Item 2. The microbial degradation promoter according to Item 1, wherein, in the general formula (1), R ¹ and R ² are the same or different and each is a linear alkyl group having 4 to 6 carbon atoms.
Item 3. Item 3. The microbial degradation promoter according to Item 1 or 2, wherein in the general formula (1), R ³ is a linear alkyl group having 1 to 8 carbon atoms.
Item 4. Item 4. The microbial degradation promoter according to any one of Items 1 to 3, wherein in the general formula (1), R ^{1 to} R ³ are the same linear alkyl group having 4 to 6 carbon atoms.
Item 5. The following general formula (1):
[In General Formula (1), R ¹ and R ² are the same or different and each represents a linear or branched alkyl group having 4 to 6 carbon atoms, and R ³ is a linear chain having 1 to 8 carbon atoms. Alternatively, it represents a branched alkyl group, and n represents an integer of 1 or more. ]
Production of a decomposition product of organic waste, comprising a step of decomposing the organic waste by coexisting a microorganism and an organic waste, wherein the microbial decomposition accelerator contains a compound represented by the formula: Method.
Item 6. Item 6. The method for producing a decomposition product of organic waste according to Item 5, wherein, in the general formula (1), R ¹ and R ² are the same or different and each is a linear alkyl group having 4 to 6 carbon atoms. .
Item 7. Item 7. The method for producing an organic waste decomposition product according to Item 5 or 6, wherein, in the general formula (1), R ³ is a linear alkyl group having 1 to 8 carbon atoms.
Item 8. In the general formula (1), R ^{1 to} R ³ are the same linear alkyl group having 4 to 6 carbon atoms, and production of a decomposition product of organic waste according to any one of Items 5 to 7 Method.
Item 9. The organic substance in any one of claim | item 5-8 whose usage-amount of the compound represented by the said General formula (1) is 0.001-5 mass parts with respect to 100 mass parts of said organic waste. A manufacturing method of waste decomposition products.
Item 10. The following general formula (1):
[In General Formula (1), R ¹ and R ² are the same or different and each represents a linear or branched alkyl group having 4 to 6 carbon atoms, and R ³ is a linear chain having 1 to 8 carbon atoms. Alternatively, it represents a branched alkyl group, and n represents an integer of 1 or more. ]
A method for decomposing organic waste, comprising using a microbial degradation accelerator comprising a compound represented by the formula:

  According to the present invention, a microbial degradation accelerator that promotes degradation of organic waste by microorganisms, a method for producing a degradation product of organic waste using the microbial degradation accelerator, and the microbial degradation accelerator are used. A method for decomposing organic waste can be provided. Moreover, the degradation product obtained by using the microbial degradation accelerator of the present invention can be effectively used as, for example, compost. Even when not used as compost, the decomposed product is effectively reduced in volume from organic waste, and therefore can be suitably used for landfilling and incineration.

2 is a photograph showing the culture results of Bacillus subtilis in each medium of Test Example 1. FIG. 4 is a photograph of Bacillus subtilis culture medium stained with Congo red in each medium of Test Example 1. FIG. 4 is a photograph of Bacillus licheniformis culture medium in each medium of Test Example 2 stained with Congo red. 10 is a graph showing changes in turbidity over time when Bacillus subtilis is cultured in each medium in Test Example 3. FIG. In Example 1 and Comparative Example 1, it is the photograph of the fallen leaf used as the organic waste used as a raw material. 6 is a graph showing a temperature change in a decomposition process in Example 1 and Comparative Example 1. 2 is a photograph of degradation product A obtained in Example 1. 2 is a photograph of decomposition product B obtained in Comparative Example 1. In Example 2 and Example 3, it is the photograph of the lawn used as the organic waste used as a raw material. It is a graph which shows the temperature change of the decomposition | disassembly process in Example 2 and Example 3. FIG. 2 is a photograph of the decomposition product C obtained in Example 2. 4 is a photograph of a decomposition product D obtained in Example 3. In Example 4 and Comparative Example 2, it is the photograph of the lawn used as the organic waste used as a raw material. It is a graph which shows the temperature change of the decomposition process in Example 4 and Comparative Example 2. 4 is a photograph of decomposition product E obtained in Example 4. 4 is a photograph of the decomposition product F obtained in Comparative Example 2. 6 is a photograph in the toilet bowl after the decomposition process in Example 5. FIG.

  The microbial degradation promoter of the present invention is characterized by containing as an active ingredient a compound represented by the above general formula (1) (hereinafter sometimes referred to as compound (1)). In the present invention, the “microorganism degradation promoter” means an additive added to organic waste in order to promote the degradation of organic waste described later by microorganisms. Hereinafter, the microbial degradation promoter of the present invention will be described in detail.

Microbial degradation promoter The organic waste microbial degradation promoter of the present invention contains a compound represented by the following general formula (1) as an active ingredient.

In the general formula (1), R ¹ and R ² are the same or different and each represents a linear or branched alkyl group having 4 to 6 carbon atoms. R ³ represents a linear or branched alkyl group having 1 to 8 carbon atoms. n represents an integer of 1 or more. The microbial degradation accelerator of the present invention may contain only one type of compound represented by the general formula (1), or may contain two or more types in combination.

Specific examples of the linear or branched alkyl group having 4 to 6 carbon atoms of R ¹ and R ² include n-butyl group, isobutyl group, sec-butyl group, tert-butyl group, n-pentyl group, isopentyl group. Group, sec-pentyl group, tert-pentyl group, neopentyl group, n-hexyl group, isohexyl group, sec-hexyl group, tert-hexyl group, neohexyl group and the like. Specific examples of the linear or branched alkyl group having 1 to 8 carbon atoms of R ³ include methyl group, ethyl group, n-propyl group, isopropyl group, n-butyl group, isobutyl group, sec-butyl. Group, tert-butyl group, n-pentyl group, isopentyl group, sec-pentyl group, tert-pentyl group, neopentyl group, n-hexyl group, isohexyl group, sec-hexyl group, tert-hexyl group, neohexyl group, n -Heptyl group, isoheptyl group, tert-heptyl group, neoheptyl group, n-octyl group, isooctyl group, tert-octyl group, neooctyl group and the like.

In the present invention, from the viewpoint of further promoting the decomposition of organic waste by microorganisms, in the general formula (1), R ¹ and R ² are the same or different and are each linear alkyl having 4 to 6 carbon atoms. It is preferably a group, and preferred specific examples include an n-butyl group, an n-pentyl group, and an n-hexyl group. From the same viewpoint, R ³ is preferably a linear alkyl group having 1 to 8 carbon atoms, more preferably a linear alkyl group having 2 to 8 carbon atoms, and 4 to 4 carbon atoms. More preferably, it is a 6 linear alkyl group. Preferable specific examples of R ³ include methyl group, ethyl group, n-propyl group, n-butyl group, n-pentyl group, n-hexyl group, n-heptyl group, n-octyl group and the like.

In the present invention, from the viewpoint of further promoting the decomposition of organic waste by microorganisms, in general formula (1), R ^{1 to} R ³ are the same linear alkyl group having 4 to 6 carbon atoms. Are particularly preferred, and preferred specific examples include an n-butyl group, an n-pentyl group, and an n-hexyl group.

  In general formula (1), n is not particularly limited as long as it is an integer of 1 or more, preferably an integer of 1 to 5, more preferably an integer of 1 to 3, and still more preferably 1.

  Among the compounds (1) contained as an active ingredient in the microbial degradation accelerator of the present invention, particularly preferred compounds are the compounds represented by the following general formulas (A) to (C) (hereinafter each referred to as the compound (A)). ~ (C)).

  Compound (1) can be produced by a known production method, for example, according to the method described in JP 2010-220607 A.

  The microbial degradation promoter of the present invention comprises compound (1) which is an active ingredient. From the viewpoint of enhancing dispersibility in organic waste and microorganisms, the microbial degradation promoter of the present invention may be diluted with water or the like. Furthermore, the microbial degradation promoter of the present invention may be diluted with a solution containing alcohols such as ethanol in addition to water, as long as the effects of the present invention are not inhibited. When diluting the microbial degradation promoter with water or the like, the concentration of the compound (1) in the diluted solution is not particularly limited and is, for example, about 1 to 50% by mass, preferably about 1 to 30% by mass, more preferably. About 1-20 mass% is mentioned. The microbial degradation promoter of the present invention may contain, for example, amino acids, polypeptides, saccharides, trace metals and the like.

  The organic waste is not particularly limited as long as it mainly contains organic matter decomposed by microorganisms. For example, woody systems such as fallen leaves, lawn, mushroom waste bed, thinned wood, wood chips, sawdust, etc. Waste; Agricultural waste such as hay, rice straw, and rot crops; Food processing industry waste such as vegetable waste and spoiled vegetables; Animal manure such as humans, birds, pigs, cattle, and horses; Examples include sludge from the sewer. In addition, the organic waste may contain organic substances and inorganic substances that are not decomposed by microorganisms.

  The microorganisms whose decomposition of organic waste is promoted by the microbial decomposition accelerator of the present invention is not particularly limited, and bacteria and fungi widely present in the natural world such as organic waste, soil and air Etc. Examples of such microorganisms include aerobic microorganisms and anaerobic microorganisms, and preferably aerobic microorganisms from the viewpoint of decomposing organic waste and composting. Specific examples of the microorganism include Bacillus spp., Actinomycetes, and wood decay fungi. One type of microorganism that decomposes organic waste may be used alone, or two or more types may be used.

  Specific examples of the genus Bacillus include Bacillus subtilis, Bacillus licheniformis, Bacillus coagulans, and Bacillus stearothers. Can be mentioned.

  Specific examples of actinomycetes include actinomycetes belonging to the genus Streptomyces, Thermoactinomyces, Actinomyces, and Thermomonospora. Examples of the actinomycetes belonging to the genus Streptomyces include Streptomyces sacchari and Streptomyces aureus. Examples of actinomycetes belonging to the genus Thermoactinomyces include Thermoactinomyces vulgaris and Thermoactinomyces monospora. Actinomyces belonging to the genus Actinomyces includes Actinomyces thermoviolaceus, Actinomyces longisporus, and the like. Examples of actinomycetes belonging to the genus Thermomonospora include Thermomonospora cruvata.

  Specific examples of the wood rot fungi include white rot fungi and brown rot fungi. Examples of white rot fungi include shiitake mushroom, oyster mushroom, maitake mushroom, kawaratake, tamogitake, enokitake and the like. In addition, examples of brown rot fungi include Prunus edulis, Namidatake, Idotake, Sarnoshikoku and the like.

  Although the details of the mechanism by which the decomposition of organic waste is promoted by the microbial degradation accelerator of the present invention are not clear, for example, it can be considered as follows. That is, by adding the microbial degradation accelerator of the present invention to organic waste, the activity of microorganisms is increased in the presence of the microbial degradation accelerator, or the growth rate of microorganisms is increased. It is thought that decomposition is promoted.

Method for producing organic waste decomposition product The method for producing an organic waste decomposition product of the present invention comprises the above microbial degradation accelerator, the above microorganisms, and the above organic waste material, It comprises a step of decomposing organic waste. In the production method of the present invention, by using the above microbial degradation promoter, the degradation of organic waste by microorganisms is promoted, and the degradation product of organic waste can be efficiently produced.

  Usually, microorganisms that decompose organic waste exist in the natural world such as organic waste, soil, and the atmosphere, and microorganisms may already adhere to the organic waste subjected to decomposition. In such a case, the production method of the present invention can be performed by adding a microbial degradation promoter to the organic waste without actively adding the microorganism to the organic waste. Moreover, in this invention, you may add microorganisms (microbe formulation) to organic waste. As such microorganisms, known microorganisms used for decomposing organic waste can be used, and commercially available products may be used.

  When the microbial degradation accelerator, the microorganism, and the organic waste are allowed to coexist, it is preferable to mix the organic waste, the microorganism, and the microbial degradation accelerator so that they are uniformly dispersed. . Examples of the mixing method include a method in which a microbial decomposition accelerator is poured from a container such as a beaker or a bucket into organic waste and stirred, and a method in which the microbial decomposition accelerator is sprayed onto the organic waste by spraying. It is done.

  In the production method of the present invention, water content may be adjusted by adding water to the organic waste to be decomposed. The amount of water at the time of mixing the microbial decomposition accelerator (at the start of the decomposition step) is not particularly limited.

  In the production method of the present invention, microbial nutrients may be further added to the organic waste to be decomposed. Microbial nutrients include those containing nitrogen, phosphorus, etc., and specifically, wheat flour, rice bran, okara, bran and the like.

  In the present invention, the C / N ratio, which is the ratio of organic carbon to nitrogen in the organic waste, is not particularly limited, and is, for example, about 5 to 300, preferably about 10 to 200. Moreover, when using the decomposition product obtained by the manufacturing method of this invention as compost, Preferably C / N ratio of decomposition product is about 5-40, More preferably, about 10-30 is mentioned.

  Although the usage-amount of the compound (1) with respect to organic waste is not restrict | limited in particular, About 0.001-5 mass parts normally with respect to 100 mass parts of organic waste, Preferably it is about 0.003-3 mass parts. More preferably, the amount is about 0.005 to 2 parts by mass.

  In the production method of the present invention, the temperature of the organic waste during the decomposition step is not particularly limited as long as the microorganism can be decomposed. In the production method of the present invention, when organic waste is being decomposed by microorganisms, heat is generated due to decomposition of the microorganisms. Therefore, the temperature of the organic waste in the decomposition step is, for example, about 40 to 90 ° C. It becomes. Moreover, although the decomposition period of the organic waste in the manufacturing method of this invention changes with kinds, quantity, etc. of organic waste, it can be normally made into about 1 week-6 months. In addition, since water | moisture content evaporates by the heat_generation | fever etc. during a decomposition | disassembly process, you may add a water | moisture content suitably during a decomposition | disassembly process. Moreover, in the manufacturing method of this invention, you may heat as needed.

  The production method of the present invention can be performed, for example, using a facility having a large area, a composting apparatus, or the like while appropriately stirring. When the microorganism that decomposes organic waste is an aerobic microorganism, the decomposition step is preferably performed while contacting the microorganism and oxygen (air) by stirring or the like. When the organic waste is stirred during the decomposition step, the stirring speed, the stirring time, the standing time, and the like can be appropriately set according to the amount of the organic waste.

  According to the production method of the present invention, at least a part of the organic waste is decomposed to lower molecular weight compounds such as carbon dioxide and ammonia to reduce the volume. Furthermore, in the decomposition step of the production method of the present invention, heat is generated by the decomposition of organic waste by microorganisms, and can be maintained at a high temperature of 55 ° C. or more for 3 days or more. It is also possible to promote the decomposition of the above harmful substances. Therefore, the decomposition product obtained by the production method of the present invention can be suitably used as compost. Moreover, since the decomposition product is volume-reduced, it may be subjected to landfill processing or incineration processing.

EXAMPLES Hereinafter, although an Example is given and this invention is demonstrated, this invention is not limited to these Examples.
Synthesis Example 1: Synthesis of compounds used in Test Examples and Examples The following compounds (A) and (B) were synthesized according to the method described in JP 2010-220607 A.

Test Example 1 (Comparative test of growth rate of Bacillus subtilis using CMC agar medium)
In the following method and conditions, the cellulose resolution by Bacillus subtilis was determined based on the medium (added medium) added with the compound (A) synthesized above and the medium not added (added medium). And compared.
<Conditions>
Medium used: CMC agar medium (polypeptone 2%, CMC (carboxymethylcellulose) 1%, yeast extract 0.1%, KH ₂ PO ₄ 0.1%, NaCl 0.5%, Na ₂ CO ₃ 0.5%, MgSO ₄ · 7H ₂ O 0.02%, agar 1.5%) CMC is Serogen 3H (average molecular weight 210,000-250,000, degree of etherification 0.55 made by Daiichi Kogyo Seiyaku Co., Ltd.) 0.65). 1% by mass of compound (A) (tributylaminoacetic acid) was added to one medium (added medium), and no compound (A) was added to the other medium (added medium).
Strains used: Bacillus subtilis
Medium temperature: 37 ° C
Staining solution: 0.1% Congo red solution 1M NaCl aqueous solution <Method>
Each medium was inoculated with bacteria and cultured at 37 ° C. for 6 days. After completion of the culture, colonies of each medium were observed. A photograph of each medium is shown in FIG. The left side of FIG. 1 is an added medium, and the right side is an additive-free medium. Next, 0.1% Congo red aqueous solution was poured into each medium and left for 15 minutes. Next, after the surface of each medium was washed with water, a 1M NaCl aqueous solution was poured into each medium and left for 15 minutes to stain and observe the decomposed portion. The results are shown in FIG. The left side of FIG. 2 is the added medium, and the right side is the non-added medium.
<Result>
In the medium supplemented with compound (A) (left photo in FIG. 1), the colonies of Bacillus subtilis were larger than in the non-added medium (photo on the right in FIG. 1). It was. In addition, as shown in FIG. 2, the CMC decomposition range was wider in the supplemented medium (left photo in FIG. 2) than in the non-added medium (right photo in FIG. 2).

Test Example 2 (Comparative test of growth rate of Bacillus licheniformis using CMC agar medium)
In the following method and conditions, the cellulose resolution by Bacillus licheniformis was not added to the medium (added medium) added with compound (A) and / or compound (B) synthesized above. Comparison was made with a medium (medium without addition).
<Conditions>
Medium used: CMC agar medium (polypeptone 2%, CMC (carboxymethylcellulose) 1%, yeast extract 0.1%, KH ₂ PO ₄ 0.1%, NaCl 0.5%, Na ₂ CO ₃ 0.5%, MgSO ₄ · 7H ₂ O 0.02%, agar 1.5%) CMC is Serogen 3H (average molecular weight 210,000-250,000, degree of etherification 0.55 made by Daiichi Kogyo Seiyaku Co., Ltd.) 0.65). In one medium, the following amount of compound (A) (tributylaminoacetic acid) and / or compound (B) (tripentylaminoacetic acid) is added (added medium), and in the other medium, compound (A) and / or Compound (B) was not added (additive-free medium).
Strains used: Bacillus licheniformis (isolated from liquid fertilizer biostart lysoboot)
The amount of compound (A) and compound (B) added to each medium is as follows:
Compound (A) and compound (B) were not added to the additive-free medium. Addition medium A1 was added so that compound (A) was 1% by mass. Addition medium B2 added so that it might become 06 mass% Addition medium B3 added so that compound (B) might become 0.03 mass% Addition medium B3 added so that compound (B) might become 0.015 mass% The added medium AB1 was added so that the compound (A) was 1% by mass and the compound (B) was 0.25% by mass. Medium temperature: 37 ° C.
Staining solution: 0.1% Congo red solution 1M NaCl aqueous solution <Method>
Each medium was inoculated with bacteria and cultured at 37 ° C. for 6 days. Next, 0.1% Congo red aqueous solution was poured into each medium and left for 15 minutes. Next, after the surface of each medium was washed with water, a 1M NaCl aqueous solution was poured into each medium and left for 15 minutes to stain and observe the decomposed portion. The results are shown in FIG.
<Result>
In any of the supplemented media (photo on the right side of FIG. 3), the colonies of Bacillus licheniformis are larger and the degradation range of CMC is larger than the non-added media (photo on the left side of FIG. 3). It was wide.

Test Example 3 (Comparative test of growth rate of Bacillus subtilis using CMC liquid medium)
Under the following method and conditions, the growth rate of Bacillus subtilis in the CMC liquid medium was compared between the case where the compound (A) synthesized above was added and the case where it was not added.
<Conditions>
Medium used: CMC liquid medium (polypeptone 2%, CMC 1%, yeast extract 0.1%, KH ₂ PO ₄ 0.1%, NaCl 0.5%, Na ₂ CO ₃ 0.5%, MgSO ₄ · 7H ₂ O 0.02%) CMC is Serogen 3H (average molecular weight 210,000-250,000, degree of etherification 0.55-0.65) manufactured by Daiichi Kogyo Seiyaku Co., Ltd. 1% by mass of compound (A) (tributylaminoacetic acid) was added to one medium (added medium), and no compound (A) was added to the other medium (added medium).
Strains used: Bacillus subtilis
Medium temperature: 37 ° C
<Method>
Each liquid medium was inoculated with bacteria and cultured at 37 ° C. for 74 hours using a biophoto recorder. A graph showing the change over time of the turbidity of the medium is shown in FIG. Furthermore, the number of bacteria was measured after the culture. The results are shown in Table 1.

  From the graph shown in FIG. 4 and the number of bacteria shown in Table 1, the growth rate of Bacillus subtilis in the medium added with the compound (A) is higher than that in the medium without the compound (A). And it became clear that it became bigger.

Example 1 (Microbial degradation test of fallen leaves, and germination test of Komatsuna seeds using degradation products)
<Degraded leaf microbial degradation test>
As the organic waste used as a raw material, 3000 g of fallen leaves (primarily cherry leaves, water content 20% by mass) shown in the photograph of FIG. 5 are used, and 3825 g of water and 200 g of flour (nitrogen content 1.3% by mass) as a nitrogen source are used. , And 175 g of a 20% by mass aqueous solution of the compound (A) as a microbial degradation promoter (1.17 parts by mass of the compound (A) with respect to 100 parts by mass of fallen leaves) The product was put into a small-sized composting experiment apparatus manufactured by the company, trade name “Kaguya Hime” (maximum effective content 12 L) and subjected to decomposition for 30 days. In addition, water was added appropriately during the 30-day microbial degradation test. The temperature change during decomposition is as shown in the graph of FIG. A photograph of degradation product A obtained after 30 days is shown in FIG. Table 2 shows values obtained by measuring the total nitrogen amount (%) and the C / N ratio of the raw material fallen leaves and the decomposition product A by elemental analysis.

<Germination test of Komatsuna seeds>
50 pieces of Komatsuna seeds were sprinkled on the decomposition product A obtained in Example 1, and distilled water was added to confirm the germination number after 48 hours. In addition, 50 komatsuna seeds were sprayed with distilled water alone, and the germination number after 48 hours was confirmed. These results are shown in Table 3.

Comparative Example 1 (Microbial degradation test of fallen leaves and germination test of Komatsuna seeds using degradation products)
<Degraded leaf microbial degradation test>
A microbial degradation test was conducted in the same manner as in Example 1 except that 3000 g of the above-described fallen leaves, 4000 g of water, and 200 g of wheat flour were used without using the compound (A), and a degradation product B was obtained. The temperature change during decomposition is as shown in the graph of FIG. Moreover, the photograph of the decomposition product B obtained after 30 days is shown in FIG. Table 2 shows the total nitrogen amount (%) and C / N ratio measured for the decomposed product B by the same method as in Example 1.

<Germination test of Komatsuna seeds>
In the same manner as in Example 1, a germination test of Komatsuna seeds was performed on the degradation product B. The results are shown in Table 3.

  From the graph of FIG. 6, in Example 1 to which the compound (A) was added, the temperature during decomposition became higher than in Comparative Example 1 to which the compound (A) was not added, and decomposition (composting) progressed faster. It became clear that Further, as is apparent from the photographs of FIGS. 7 and 8, the degradation product A of Example 1 is darker than the degradation product B of Comparative Example 1 also in the appearance of the degradation product after the microbial degradation test. The shape was also fine. Furthermore, as shown in Table 2, Example 1 was also good with respect to the total nitrogen amount and the C / N ratio. As shown in Table 3, in Example 1, in the germination test of Komatsuna seeds, the germination rate was the same as in Comparative Example 1 and germination with only distilled water, and germination of Komatsuna seeds with Compound (A). Growth inhibition was not observed.

Example 2 (Microbial Degradation Test of Lawn and Germination Test of Komatsuna Seed Using Degradation Product)
<Microbial degradation test of lawn>
As the organic waste used as a raw material, 4000 g of the lawn (golf lawn, water 25% by mass) shown in the photograph of FIG. 9 is used instead of fallen leaves, 3000 g of water is used for this, and rice bran 200 g is used instead of flour as the nitrogen source. And 175 g of a 20% by mass aqueous solution of the compound (A) as a microbial degradation accelerator (0.875 parts by mass of the compound (A) with respect to 100 parts by mass of lawn) was the same as in Example 1. Then, a microbial degradation test was performed to obtain a degradation product C. The temperature change during decomposition is as shown in the graph of FIG. A photograph of degradation product C obtained after 30 days is shown in FIG. In addition, Table 4 shows values obtained by measuring the total nitrogen amount (%) and the C / N ratio of the lawn and decomposition product C used as raw materials in the same manner as in Example 1.

<Germination test of Komatsuna seeds>
In the same manner as in Example 1, the decomposition product C obtained in Example 2 was subjected to a germination test of Komatsuna seeds. The results are shown in Table 5.

Example 3 (Microbial Degradation Test of Lawn and Germination Test of Komatsuna Seed Using Degradation Product)
<Microbial degradation test of lawn>
4000 g of the above lawn, 2825 g of water, 200 g of rice bran, and a mixture of 166.25 g of a 20% by mass aqueous solution of the compound (A) as a microbial degradation accelerator and 8.75 g of a 20% by mass aqueous solution of the compound (B) (100 parts by mass of the lawn On the other hand, a microbial degradation test was conducted in the same manner as in Example 2 except that the total of the compound (A) and the compound (B) was 0.875 parts by mass, and a degradation product D was obtained. . The temperature change during decomposition is as shown in the graph of FIG. A photograph of the degradation product D obtained after 30 days is shown in FIG. Table 4 shows values obtained by measuring the total nitrogen amount (%) and C / N ratio of the decomposition product D in the same manner as in Example 1.

<Germination test of Komatsuna seeds>
In the same manner as in Example 1, the decomposition product D obtained in Example 3 was subjected to a germination test of Komatsuna seeds. The results are shown in Table 5.

  From the graph of FIG. 10, in Example 2 to which the compound (A) was added and in Example 3 to which both the compound (A) and the compound (B) were added, the temperature during decomposition rose to 70 ° C. or higher. And it became clear that decomposition (composting) is progressing quickly. Further, as is apparent from the photographs of FIGS. 11 and 12, both the degradation product C of Example 2 and the degradation product D of Example 3 are dark in color and in the appearance of the degradation product after the microbial degradation test. It was fine. Furthermore, as shown in Table 4, Examples 2 and 3 were also good in terms of the total nitrogen amount and C / N ratio. As shown in Table 5, in Examples 2 and 3, also in the germination test of Komatsuna seeds, the germination rate was the same as in Example 1, Comparative Example 1, germination with distilled water alone, and the compound ( No germination / growth inhibition of Komatsuna seeds by A) or compound (B) was observed.

Example 4 (Microbial Degradation Test of Lawn and Germination Test of Komatsuna Seed Using Degradation Product)
<Microbial degradation test of lawn>
As an organic waste as a raw material, 4000 g of the lawn (golf lawn, water 25% by mass) shown in the photograph of FIG. 13 is used, and 2996.5 g of water, 200 g of rice bran, and a compound (A) as a microbial degradation accelerator. A microbial decomposition test was performed in the same manner as in Example 2 except that 3.5 g of the 20 wt% aqueous solution of the compound (0.0175 parts by mass of the compound (A) relative to 100 parts by mass of the lawn) was used. A decomposition product E was obtained. The temperature change during decomposition is as shown in the graph of FIG. A photograph of degradation product E obtained after 30 days is shown in FIG. Table 6 shows values obtained by measuring the total nitrogen amount (%) and the C / N ratio of the lawn and decomposition product E used as raw materials in the same manner as in Example 1.

<Germination test of Komatsuna seeds>
In the same manner as in Example 1, the decomposition product E obtained in Example 4 was subjected to a germination test of Komatsuna seeds. The results are shown in Table 7.

Comparative Example 2 (Microbial Degradation Test of Lawn and Germination Test of Komatsuna Seed Using Degradation Product)
<Microbial degradation test of lawn>
A microbial degradation test was performed in the same manner as in Example 4 except that 4000 g of raw material lawn in Example 4, 3000 g of water, and 200 g of rice bran were used without using the compound (A), and a degradation product F was obtained. The temperature change during decomposition is as shown in the graph of FIG. A photograph of degradation product F obtained after 30 days is shown in FIG. Table 6 shows values obtained by measuring the total nitrogen amount (%) of the decomposition product F and the C / N ratio in the same manner as in Example 1.

<Germination test of Komatsuna seeds>
In the same manner as in Example 1, the decomposition product F obtained in Comparative Example 2 was subjected to a germination test for Komatsuna seeds. The results are shown in Table 7.

  From the graph of FIG. 14, even in Example 4 where the compound (A) was used at a very low concentration (the concentration of the compound (A) was 100 ppm in the total amount of lawn, water and the aqueous solution), The temperature rose to 70 ° C. or higher, and it became clear that decomposition (composting) was progressing quickly. Further, as is apparent from the photographs of FIGS. 15 and 16, the degradation product E of Example 4 is darker than the degradation product F of Comparative Example 2 also in the appearance of the degradation product after the microbial degradation test. The shape was also fine. Furthermore, as shown in Table 6, Example 4 was also good with respect to the total nitrogen amount and the C / N ratio. As shown in Table 7, in Example 4, in the germination test of Komatsuna seeds, the germination rate was the same as in the case of germination in Comparative Example 2, and germination / growth inhibition of Komatsuna seeds by the compound (A) was I was not able to admit.

Example 5 (Microbial degradation test in biotoilet)
Only fine sawdust is put into the toilet bowl, and 30 kg of sawdust is put into the toilet bowl of a biotoilet (toilet tank capacity 150 L) that is heated to 40-80 ° C. with mechanical stirring, and further a compound as a microbial degradation accelerator 175 g of a 20 mass% aqueous solution of (A) (0.117 parts by mass of the compound (A) with respect to 100 parts by mass of sawdust) was sprayed on the sawdust. This biotoilet was driven in the parking lot for 3 months. The total number of users of biotoilet during the test period was about 1100, about 600 people used in the first month, and about 500 people used in the next two months. As a result, a smooth and good degradation product was obtained in the toilet bowl both after one month and after three months. The photograph in the toilet bowl of the biotoilet after 3 months is shown in FIG. In this type of biotoilet, the decomposition product in the stool is usually mushy with a lot of moisture when it is used by about 1000 people (about 3 months), and the stool needs to be replaced. On the other hand, as is clear from FIG. 17, in the biotoilet of Example 5, there was no stool or urine residue, the entire sawdust became blackish brown, and a smooth and good degradation product with little moisture was obtained. It was still ready for use.

Claims (7)

The following general formula (1):
[In General Formula (1), R ^{1 to} R ³ represent the same linear alkyl group having 4 or 5 carbon atoms, and n is 1. ]
An organic waste microbial degradation promoter comprising a compound represented by the formula: The microbial degradation promoter according to claim 1, wherein the organic waste is woody waste, agricultural waste, animal manure, or sludge from water and sewage. The following general formula (1):
[In General Formula (1), R ^{1 to} R ³ represent the same linear alkyl group having 4 or 5 carbon atoms, and n is 1. ]
Production of a decomposition product of organic waste, comprising a step of decomposing the organic waste by coexisting a microorganism and an organic waste, wherein the microbial decomposition accelerator contains a compound represented by the formula: Method. Decomposition | disassembly of the organic waste of Claim 3 whose usage-amount of the compound represented by the said General formula (1) is 0.001-5 mass parts with respect to 100 mass parts of said organic waste. Manufacturing method. The manufacturing method of the decomposition product of the organic waste of Claim 3 or 4 whose said organic waste is a sludge from a woody waste, agricultural waste, animal excrement, or water and sewage. The following general formula (1):
[In General Formula (1), R ^{1 to} R ³ represent the same linear alkyl group having 4 or 5 carbon atoms, and n is 1. ]
A method for decomposing organic waste, comprising using a microbial degradation accelerator comprising a compound represented by the formula: The method for decomposing organic waste according to claim 6, wherein the organic waste is woody waste, agricultural waste, animal manure, or sludge from water and sewage.