JP6157289B2 - Plant sterilization method - Google Patents

Description

  The present invention relates to a method for sterilizing plants.

  Plant pathogenic bacteria cause growth inhibition and quality degradation on crops and horticultural plants, and cause great harm to plants. Currently, chemical synthetic pesticides such as organochlorine (TPN, captan), benzimidazole, acid amide, guanidine, carbamate, organophosphorus, and organic copper are mainly used to control pathogens. Chemically synthesized pesticides develop drug resistance against pathogens due to excessive spraying of pesticides, and therefore, pesticides that are safe and difficult to have drug resistance are desired. In addition, excessive application of chemically synthesized pesticides is concerned about the burden on the environment and the burden on workers.

  From this point of view, it is desired to develop a pesticide having an excellent control effect, and a technique capable of controlling phytopathogenic fungi from a viewpoint different from conventional chemically synthesized pesticides is being studied. As one of them, it has been proposed to use a so-called surfactant as an agrochemical. For example, in Patent Document 1, a polyhydric alcohol fatty acid ester nonionic surfactant (A), a polyhydric alcohol fatty acid ester alkylene oxide adduct nonionic surfactant, and a resin acid nonionic surfactant are selected. An agrochemical composition containing one or more (B) is disclosed. Patent Document 2 discloses an agricultural and horticultural insecticidal fungicide composition containing a medium chain triglyceride having 8 to 10 carbon atoms and a surfactant such as polyoxyethylene fatty acid ester.

On the other hand, as an antibacterial agent composition suitable for a product having a hard surface, an antibacterial agent composition containing an alcohol having 8 to 14 carbon atoms, a surfactant, and hydrogen peroxide under predetermined conditions is known (Patent Document 3). ).
In addition, as an antibacterial agent that can be used in foods, an antibacterial agent containing a polyglycerol fatty acid ester and a polyglycerol condensed ricinoleate is known (Patent Document 4).
In addition, for the purpose of enhancing the efficacy of agricultural chemicals, an efficacy enhancing agent composition for agricultural chemicals containing a polyoxyethylene fatty acid ester or a polyglycerol fatty acid ester and an aliphatic alcohol having 8 to 14 carbon atoms is known (patent document). 5).

JP 2006-137728 A JP 11-29411 A JP 2010-138102 A JP 2001-316206 A JP 2012-184187 A

  The subject of this invention is providing the sterilization method of the plant excellent in the sterilization effect.

The present invention substantially does not contain an agrochemical active ingredient that is an active ingredient of a bactericide, and is one or more compounds (A) selected from the following (A1) and (A2), and one or more selected from the following (B1) A plant containing a compound (B) and water, wherein the total amount of the compound (A) and the compound (B) is 2,000 ppm or more and 100,000 ppm or less. The present invention relates to a sterilization method.
(A1): Polyoxyethylene fatty acid ester (however, the number of carbon atoms of the fatty acid is 8 or more and 16 or less, and the average added mole number of ethylene oxide is 5 or more and 40 or less.)
(A2): (Poly) glycerin fatty acid ester (however, the number of carbon atoms of the fatty acid is from 8 to 16, and the average degree of condensation of glycerin is from 1 to 3)
(B1): aliphatic alcohol represented by the following general formula (B1)
R ^1b —OH (B1)
(In the formula, R ^1b represents a linear or branched alkyl group having 8 to 14 carbon atoms.)

  ADVANTAGE OF THE INVENTION According to this invention, the sterilization method of the plant excellent in the sterilization effect is provided.

  The plant sterilization method of the present invention (hereinafter sometimes simply referred to as “the sterilization method of the present invention”) includes a step of applying a treatment liquid containing a compound (A) and a compound (B) at a predetermined concentration to a plant. Have. The combined use of the compound (A) and the compound (B) significantly improves the bactericidal properties against plant disease-causing fungi that grow on the plant surface and inside the plant. The reason for this is that the chemical spread of the medicinal solution on the plant surface is improved and the permeability to the phytopathogenic fungus is improved, and the cell membrane of the phytopathogenic fungus is physically controlled by the surface activity of the compound (A) and the compound (B). It is estimated that the pathogenic bacteria will be killed by destroying them. Since the pathogenic bacteria are killed by the physical action of membrane destruction, it is considered that the pathogenic bacteria are less likely to acquire drug resistance than the pesticides that kill the pathogenic bacteria by biochemical action.

<Compound (A)>
In the present invention, one or more compounds (A) selected from the following (A1) and (A2) are used. The compound (A) is preferably one or more compounds selected from (A2) from the viewpoint of improving the bactericidal effect.
(A1): Polyoxyethylene fatty acid ester (wherein the fatty acid has 8 to 16 carbon atoms, and the average added mole number of ethylene oxide is 5 to 40) (hereinafter referred to as compound (A1))
(A2): (Poly) glycerin fatty acid ester (wherein the fatty acid has 8 to 16 carbon atoms, and the average degree of condensation of glycerin is 1 to 3) (hereinafter referred to as compound (A2))

  Compound (A1) is a polyoxyethylene fatty acid ester. The number of carbon atoms of the fatty acid in the compound (A1) is 8 or more and 16 or less from the viewpoint of improving the bactericidal effect. From the viewpoint of improving the bactericidal effect, the number of carbon atoms of the fatty acid in the compound (A1) is preferably 10 or more, preferably 14 or less, and more preferably 12 or less. The fatty acid in the compound (A1) preferably has a linear or branched alkyl group or alkenyl group, and more preferably a linear alkyl group, from the viewpoint of improving the bactericidal effect. Examples of the fatty acid in the compound (A1) include caprylic acid, capric acid, lauric acid, myristic acid, palmitic acid and the like. From the viewpoint of improving the bactericidal effect, capric acid and lauric acid are preferable, and lauric acid is more preferable. It is. Moreover, the average addition mole number of ethylene oxide in a compound (A1) is 5 or more and 40 or less from a viewpoint of a bactericidal effect improvement. From the viewpoint of improving the bactericidal effect, the average number of moles of ethylene oxide added in the compound (A1) is preferably 6 or more, more preferably 8 or more, further preferably 10 or more, preferably 30 or less, preferably 20 or less, 15 The following is more preferable. The ester is preferably a monoester from the viewpoint of improving the bactericidal effect.

  Compound (A2) is a (poly) glycerin fatty acid ester. Here, “(poly) glycerin fatty acid ester” means “one or more selected from the group consisting of glycerin fatty acid ester and polyglycerin fatty acid ester”. Carbon number of the fatty acid in the compound (A2) is 8 or more and 16 or less from the viewpoint of improving the bactericidal effect. The number of carbon atoms of the fatty acid in the compound (A2) is preferably 10 or more, more preferably 12 or less, and even more preferably 10 from the viewpoint of improving the bactericidal effect. The fatty acid in the compound (A2) preferably has a linear or branched alkyl group or alkenyl group, and more preferably a linear alkyl group, from the viewpoint of improving wettability and improving the bactericidal effect. Examples of the fatty acid include caprylic acid, capric acid, lauric acid, myristic acid, palmitic acid and the like. From the viewpoint of improving the bactericidal effect, capric acid and lauric acid are preferable, and capric acid is more preferable. In the compound (A2), from the viewpoint of improving the bactericidal effect, the average condensation degree of glycerin is 1 or more and 3 or less, preferably 1 or 2, and preferably 1. Further, from the viewpoint of improving the bactericidal effect, the form of the ester bond is preferably a monoester form or a diester form, and more preferably a monoester form.

<Compound (B)>
In the present invention, an aliphatic alcohol represented by the following general formula (B1) is used as the compound (B).
R ^1b —OH (B1)
(In the formula, R ^1b represents a linear or branched alkyl group having 8 to 14 carbon atoms.)
In the general formula (B1), R ^1b is a linear or branched alkyl group having 8 to 14 carbon atoms, preferably a straight chain having 8 to 14 carbon atoms, from the viewpoint of improving the bactericidal effect. It is an alkyl group. R ^1b is preferably a linear alkyl group having 8 or more and 12 or less carbon atoms, more preferably a linear alkyl group having 8 or more and 10 or less carbon atoms, and more preferably carbon from the viewpoint of improving the bactericidal effect. It is a linear alkyl group of several tens. Specific examples of the compound (B2) include octyl alcohol, decyl alcohol, lauryl alcohol (dodecyl alcohol), myristyl alcohol (tetradecyl alcohol) and the like. From the viewpoint of improving the herbicidal effect, octyl alcohol, decyl are preferable. Alcohol and lauryl alcohol are preferable, and decyl alcohol is more preferable.

<Processing liquid>
In the present invention, a treatment liquid containing the compound (A), the compound (B) and water and having a total content of the compound (A) and the compound (B) of 2,000 ppm or more and 100,000 ppm or less is used.

  From the viewpoint of improving the bactericidal effect, the total content of the compound (A) and the compound (B) in the treatment liquid is 2,000 ppm or more, preferably 3,000 ppm or more, preferably 4,000 ppm or more, 500 ppm or more is preferable, and from the viewpoint of improving the bactericidal effect and economy, it is 100,000 ppm or less, preferably 80,000 ppm or less, preferably 60,000 ppm or less, more preferably 50,000 ppm or less, and 30,000 ppm or less. Is preferable, and 15,000 ppm or less is preferable.

  From the viewpoint of improving the bactericidal effect, the mass ratio of the compound (A) to the compound (B) in the treatment liquid is preferably (0.1) or more, more preferably 0.2 or more, from the viewpoint of improving the bactericidal effect. 0.3 or more is more preferable, 0.4 or more is more preferable, 0.5 or more is more preferable, 0.7 or more is more preferable, 10 or less is preferable, 5 or less is more preferable, and 3 or less is more preferable. 2 or less is more preferable, and 1.5 or less is more preferable.

<Plant sterilization method>
The sterilization method of the present invention does not substantially contain any pesticidal active ingredient selected from the active ingredients of the sterilizing agent, contains the compound (A), the compound (B) and water, the compound (A) and A step of applying to the plant a treatment liquid having a total content of the compound (B) of 2,000 ppm to 100,000 ppm.

As a process of applying the treatment liquid according to the present invention to a plant, for example, (1) the treatment liquid is sprayed on a plant, for example, a leaf surface, a stem, or a fruit of a plant by a hand sprayer, a boom sprayer, air spraying, or the like. Process,
(2) A step of directly applying the treatment liquid to a plant, for example, a leaf, stem, or fruit,
(3) A step of irrigating the soil with soil using a liquid fertilizer supply device, etc.
(4) In hydroponics, the process etc. which apply | coat to the root the hydroponics liquid in which the sum total of content of a compound (A) and a compound (B) exists in the said predetermined range is mentioned. As in (1), a step of spraying the treatment liquid on plants is preferable.

  From the viewpoint of improving the bactericidal effect, the treatment liquid is preferably 3L / 10a or more, more preferably 5L / 10a or more, more preferably 7L / 10a or more, still more preferably 10L / 10a or more, still more preferably 20L / 10a or more, More preferably 30L / 10a or more, still more preferably 40L / 10a or more, and from the viewpoint of improving the bactericidal effect and economy, preferably 300L / 10a or less, more preferably 250L / 10a or less, more preferably 200L / 10a or less. More preferably, it can be sprayed at a ratio of 150 L / 10a or less, more preferably 120 L / 10a or less.

  The treatment liquid used in the present invention may contain components other than the compound (A), the compound (B), and water. For example, you may contain the component chosen from a chelating agent, a pH adjuster, inorganic salts, and a thickener as needed.

  In the present invention, from the viewpoint of improving the bactericidal effect among the components other than water in the treatment liquid, the proportion of the compound (A) and the compound (B) is 20% by mass or more, further 30% by mass or more, and further 50% by mass. % Or more, more preferably 70% by mass or more, still more preferably 90% by mass or more, and even more preferably 95% by mass or more.

  The treatment liquid used in the present invention does not substantially contain an agrochemical raw material that is an active ingredient of a bactericide from the viewpoint of improving the bactericidal effect. This pesticide active ingredient refers to an active ingredient of a fungicide, and the compound (A) and the compound (B) are not included in the pesticide active ingredient. In addition, regarding the processing solution, “substantially no pesticide active substance” means that the content of the pesticide active ingredient is a content (for example, standard use amount) for expressing the intended medicinal effect of the pesticide active ingredient. In the present invention, the case where it is 100 ppm or less, more preferably 50 ppm or less, and even more preferably 10 ppm or less in the processing solution is defined as “substantially free of an agrochemical base”. Can do.

  Pesticide active ingredients that are active ingredients of fungicides include sulfur-based dineb (zinc ethylene bisdithiocarbamate), mannebu (manganese ethylene bisdithiocarbamate), and benzimidazole as benomyl (methyl-1- (butylcarbamoyl)- 2-benzimidazole carbamate), dicarboximide-based vinclozolin (3- (3,5-dichlorophenyl) -5-methyl-5-vinyl-1,3-oxazolidine-2,4-dione), iprodione (3- ( 3,5-dichlorophenyl) -N-isopropyl-2,4-dioxoimidazolidine-1-carboxamide), procimidone (N- (3,5-dichlorophenyl) -1,2-dimethylcyclopropane-1,2-di) Carboximide) and other triazines (2,4-dichloro-6- ( -Chloroanilino) -1,3,5-triazine), Trifumizole ((E) -4-Chloro-α, α, α-trifluoro-N- (1-imidazol-1-yl-2-propoxyethylidan)- o-toluidine), metalaxyl (methyl-N- (2-methoxyacetyl) -N- (2,6-xylyl) -D, L-alaninate), organic copper (Oxine-copper), cupric hydroxide (co Side Bordeaux, etc.), antibiotic fungicides (streptomycin, tetracycline, polyoxy, blasticidin S, kasugamycin, validamycin) and the like. Furthermore, as an agrochemical raw material which is an active ingredient of a disinfectant that is preferably not contained in the treatment liquid according to the present invention, organic copper (Oxine-copper), cupric hydroxide, trifumisol ((E) -4-chloro-) α, α, α-trifluoro-N- (1-imidazol-1-yl-2-propoxyethylidan) -o-toluidine), iprodione (3- (3,5-dichlorophenyl) -N-isopropyl-2, 4-dioxoimidazolidine-1-carboxamide).

  Examples of the plant to be subjected to the plant sterilization method of the present invention include plants cultivated as agricultural crops. Specifically, for fruits and vegetables, cucumbers, pumpkins, watermelons, melons, tomatoes, eggplants, peppers, strawberries, okra, sweet beans, broad beans, peas, green beans, corn, etc., leafy vegetables, cabbage, tsukena, chingensai, cabbage, Cauliflower, broccoli, cabbage, onion, leek, garlic, rakkyo, leek, asparagus, lettuce, salad na, celery, spinach, garlic, parsley, beeswax, seri, udo, myouga, burdock, perilla, etc. , Turnip, burdock, carrot, potato, taro, sweet potato, yam, ginger, lotus root and the like. In addition, it can also be used for rice, wheat, and flowering plants.

  The plant sterilization method of the present invention is a plant disease selected from cabbage black soot disease, wheat eye spot disease, apple leaf spot disease, pepper spot disease, cucumber anthracnose disease, chingensai soft rot disease, pear black spot disease and tomato ring disease It is preferable to sterilize the causative bacteria. Furthermore, it is preferable to sterilize causative bacteria of cabbage black soot disease.

  The plant sterilization method of the present invention is preferably directed to a plant selected from cabbage, wheat, apples, peppers, cucumbers, pak choi, pears and tomatoes, more preferably cabbage.

  In the plant sterilization method of the present invention, the treatment liquid is preferably applied to the plant being cultivated. Furthermore, it is preferable that the treatment liquid is sprayed on the plant being cultivated.

[Sterilization test 1]
Cabbage was cultivated in a 12 cm pot until the fifth leaf stage. A spore suspension of cabbage black soot disease (Alternaria brassicicola) (the number of bacteria was 10 ⁷ cells / mL) was sprayed on cabbage at a spray rate of 50 L / 10a. The compound (A) and the compound (B) shown in Tables 1 and 2 were mixed at a mass ratio shown in Table 3 to prepare a treatment solution diluted with water to a predetermined concentration. The treatment liquid was sprayed on the cabbage after 3 days after being spread at 25 ° C. and 90% relative humidity after spraying the fungus at the spraying amount shown in Table 3. Next, the pot was allowed to stand at 25 ° C. and 85% relative humidity, and after one week, the number of lesions was counted, and the control value was calculated using the following formula. The higher the value of the control value, the higher the bactericidal effect.
Control value (%) = {1− (number of lesions in treated area / number of lesions in untreated area)} × 100
From the results in Table 3, it was confirmed that the sterilization effect can be improved according to the sterilization method of the present invention.

[Sterilization test 2]
Wheat was cultivated in 12 cm pots until the fifth leaf stage. A spore suspension of Pseudocercosporella herpotrichoides (the number of bacteria was 10 ⁷ cells / mL) was applied to wheat at an application rate of 50 L / 10a. The compound (A) and the compound (B) shown in Tables 1 and 2 were mixed at a mass ratio shown in Table 3 to prepare a treatment solution diluted with water to a predetermined concentration. The treatment liquid was sprayed on the wheat after 3 days after being spread at 25 ° C. and 90% relative humidity after spraying the fungus at the spraying amount shown in Table 3. Next, the pot was allowed to stand at 25 ° C. and 85% relative humidity, and after one week, the number of lesions was counted, and the control value was calculated using the formula used in sterilization test 1. The higher the value of the control value, the higher the bactericidal effect.
From the results in Table 3, it was confirmed that the sterilization effect can be improved according to the sterilization method of the present invention.

[Sterilization test 3]
An apple deciduous leaf (Alternaria mali) spore suspension (the number of bacteria was 10 ⁷ / mL) was sprayed on the leaves of apple fruit trees in an outdoor planted state at a spraying amount of 50 L / 10a. The compound (A) and the compound (B) shown in Tables 1 and 2 were mixed at a mass ratio shown in Table 3 to prepare a treatment solution diluted with water to a predetermined concentration. The treatment solution was sprayed on the apples at the spraying amount shown in Table 3 three days after the spraying of the fungus. One week later, the number of lesions was counted, and the control value was calculated using the formula used in sterilization test 1. The higher the value of the control value, the higher the bactericidal effect.
From the results in Table 3, it was confirmed that the sterilization effect can be improved according to the sterilization method of the present invention.

(Note 1) Alkyl polyglucoside: mixed linear alkyl group having an alkyl group of 10 to 16 carbon atoms, average glucose condensation degree of 1.4

[Sterilization test 4]
Each plant was cultivated in a 12 cm pot until the fifth leaf stage. Spore suspensions (fungi) of plant pathogens (Perco spotora (Cercospora capsici), cucumber anthracnose (Colletotrichumlagenarium), chingensai soft rot (Erwinia sp), pear black rot (Venturia nashicola), tomato ring rot (Alternaria Solano)) The number was 10 ⁷ / mL) and sprayed on the leaves of each corresponding plant (green pepper, cucumber, chingensai, pear, tomato) at a spraying amount of 50 L / 10a. The compound (A) and the compound (B) shown in Tables 1 and 2 were mixed at a mass ratio shown in Table 4 to prepare a treatment solution diluted with water to a predetermined concentration. The treatment liquid was sprayed onto the plants 3 days after being left at 25 ° C. and 90% relative humidity after spraying the fungus at the spraying amount shown in Table 4. One week later, the number of lesions was counted, and the control value was calculated using the formula used in sterilization test 1. The higher the value of the control value, the higher the bactericidal effect.
From the results of Table 4, it was confirmed that according to the sterilization method of the present invention, the sterilization effect can be improved.

[Sterilization test 5]
The disinfectant and the concomitant components shown in Table 5 were added at the time of preparation of the potato dextrose medium (PDA medium) so as to have a predetermined concentration to prepare a disinfectant-containing PDA medium. An appropriate amount of a spore suspension of cabbage black soot disease (Alternariabrassicicola) (the number of bacteria was 10 ⁷ cells / mL) was applied to the prepared medium and cultured at 23 ° C. and 60% relative humidity for 7 days. Then, the sterilization performance point was evaluated by visual observation of the ratio of the area of the portion not growing on the PDA medium and the area of the entire petri dish. Judgment criteria are: Ungrown: 100 points, three-quarter ungrown area ratio: 75 points, half ungrown area ratio: 50 points, quarter ungrown area ratio: 25 points, whole petri dish Growth: 0 points. The number of repetitions was 7, and evaluation was performed based on the average value. The results are shown in Table 5.

  As shown in Table 5, in Test Example 1-1 in which the compound (A) and the compound (B) were used in combination, a higher bactericidal effect was obtained than in the case of using a chemically synthesized bactericide alone (Reference Example 1-1). It is done. As in Reference Example 1-2, when a chemical synthesis bactericidal agent is used in combination with Compound (A) and Compound (B), the bactericidal effect is improved, but in Test Example 1-1, this is equivalent to Reference Example 1-2. Bactericidal effect is obtained. That is, it was confirmed that excellent fungicide performance was exhibited by using the compound (A) and the compound (B) according to the present invention in combination without using a fungicide which is an agrochemical base.

(Note 2) Total concentration of compound (A) and compound (B)

Claims (7)

The content of the active ingredient of the pesticide which is an active ingredient of the bactericide is 100 ppm or less, one or more compounds (A) selected from the following (A1) and (A2), one or more compounds selected from the following (B1) ( containing B) and water, the compound (a) and the compound (B) of the total content is more than 2,000 ppm, Ri der below 100,000 ppm, the weight ratio of compounds (a) and (B), A method for sterilizing a plant comprising a step of applying a treatment liquid of compound (A) / compound (B) that is 0.3 or more and 3 or less to a plant, comprising cabbage black soot disease, wheat eye spot disease, apple A plant sterilization method for sterilizing a causative agent of a plant disease selected from deciduous leaf spot disease, bell pepper spot disease, cucumber anthracnose disease, chingensai soft rot disease, pear black spot disease and tomato ring rot disease.
(A1): Polyoxyethylene fatty acid ester (however, the number of carbon atoms of the fatty acid is 8 or more and 16 or less, and the average added mole number of ethylene oxide is 5 or more and 40 or less.)
(A2): (Poly) glycerin fatty acid ester (however, the number of carbon atoms of the fatty acid is from 8 to 16, and the average degree of condensation of glycerin is from 1 to 3)
(B1): aliphatic alcohol represented by the following general formula (B1)
R ^1b —OH (B1)
(In the formula, R ^1b represents a linear or branched alkyl group having 8 to 14 carbon atoms.)   The plant sterilization method according to claim 1, wherein the total content of the compound (A) and the compound (B) in the treatment liquid is 4,000 ppm or more and 60,000 ppm or less.   The plant sterilization method according to claim 1 or 2, wherein the treatment liquid is applied to the plant by spraying, and the treatment liquid is sprayed at a rate of 7L / 10a or more and 200L / 10a or less. The plant sterilization method according to any one of claims 1 to 3 , wherein the compound (A) is one or more compounds selected from (A2). The method for sterilizing a plant according to any one of claims 1 to 4 , wherein a causative bacterium of cabbage black soot disease is sterilized. The plant sterilization method according to any one of claims 1 to 5 , wherein the treatment liquid is applied to a plant under cultivation. The method for sterilizing a plant according to any one of claims 1 to 6 , wherein the treatment liquid is sprayed onto a plant under cultivation.