KR101817513B1 - Composition for preventing or controlling plant fungal diseases - Google Patents

Abstract

The present invention relates to a composition for controlling a fungal disease of a plant, and more specifically, the present invention relates to a composition for controlling a fungal disease of plant fungus comprising clove oil and pelargonic acid which are components derived from a natural product, A fertilizer containing the composition, and a method for controlling a plant fungal disease using the composition.
The present invention has an excellent control effect even in zucchini powdery mildew, red mildew powder, sesame powdery mildew, strawberry gray mold mildew and tomato leaf mold mildew, and since there are no side effects including components isolated from natural materials, Which can be widely used.

Description

Technical Field [0001] The present invention relates to a composition for controlling plant fungal diseases,

More particularly, the present invention relates to a natural germicidal composition for controlling a fungal disease of plant fungi, which comprises clove oil and pelargonic acid, an agricultural chemical preparation containing the composition, a fertilizer containing the composition And a method of controlling plant fungal diseases using the composition.

Plant fungal diseases occur not only in crops but also in trees, and are also common in weeds. Most molds form mycelium, grow back to form spores, and cause gangrene, necrosis or abnormal division of a part of the host plant or whole plant tissue. Diseased plants are symptomatic of leaf spot, blight, ulcer, bruise, root rot, leaf and stalks are wilted, and fruit quality is lowered.

On the other hand, excessive use of chemical pesticides is a major cause of environmental problems such as deterioration of soil pollution and water pollution, disturbance of ecosystem due to reduction of soil microorganisms and natural enemies. The use of more pesticides to remove resistant pests or the use of more toxic pesticides makes environmental problems worse, and pesticide residues in crops are a fatal hazard to humans. For example, the chemical disinfectants pentiofilad, pentrazamide, and the like are known to exhibit side effects such as reproductive toxicity, teratogenicity, and carcinogenicity.

As a result, studies on pesticides using a low toxic or nontoxic natural substance as a substitute for chemical pesticides have been actively conducted. Especially, there have been many studies on powdery mildew occurring in cultivation of red beans and sesame seeds. Korean Patent Publication No. 10-1464335 discloses that a composition containing an extract of Ganoderma lucidum and Rose geranium is able to control powdery mildew in plants. Korean Patent Publication No. 10-0691741 discloses a composition comprising extracts of clifaspol, emodine, berberine, Has been disclosed to be capable of controlling powdery mildew in plants. However, research on controlling plant fungal diseases with components derived from natural products is still in its infancy, and development of a wider variety of components is required.

Under these circumstances, the inventors of the present invention have made intensive researches to develop a composition that exhibits a safe and excellent plant fungal disease control effect, including a component derived from a natural product. As a result, it has been found that a composition comprising clove oil and pelargonic acid And the present invention was completed.

It is an object of the present invention to provide a composition for controlling plant fungal diseases comprising clove oil and pelargonic acid.

Another object of the present invention is to provide an agricultural chemical preparation containing the composition.

It is a further object of the present invention to provide a fertilizer comprising the composition.

It is another object of the present invention to provide a method for controlling plant fungal diseases using the composition.

The present inventors focused their attention on the fact that clods oil is used in the medical field such as preservatives in order to solve the environmental problem and various side effects of the chemical sterilizing agent and to provide safe and efficacious products including clenical oil and pelargonic acid A composition for controlling plant fungal diseases has been developed. As a result of spraying the composition with water on tomatoes of zucchini, red beans or sesame seeds, gray-scale fungus-infested strawberry, or leaf fungus disease, it was found that all three plants exhibited more than 80% control effect against fungal diseases , And it was confirmed that the same effect as the conventional chemical pesticide was obtained.

From these results, it can be seen that the natural disinfecting composition containing clods oil and pelargonic acid greatly suppresses the occurrence of fungal diseases of zucchini, red bean, sesame, strawberry or tomato.

The natural germicidal compositions comprising clove oil and pelargonic acid provided by the present invention have not been known at all and have been developed for the first time by the present inventors.

In order to accomplish the above object, the present invention provides, as one embodiment, a composition for controlling a fungal disease of plants, comprising clove oil and pelargonic acid.

The term "clove oil " of the present invention can be extracted from various parts of the clove ( Eugenia caryophyllata ) (for example, leaves, shoots, roots, stems, seeds and the like) It means volatile oil. Specifically, the clove oil may be extracted from leaves and seeds of cloves. Clove oil is used for many purposes, and it is also safe food equivalent to the substance added directly to food. Clove oil has several active ingredients, and three important active ingredients are eugenol, isougenol and methyl ureaol. The clove oil used in the present invention may specifically include, but is not limited to, 50 to 90% by weight of eugenol, 0 to 50% by weight of isougenol and methyl eugenol. Other ingredients included in the clove oil useful in the present invention include b-cariophilene (0 to 15% by weight), a-fumarate (0 to 10% by weight), eugenol acetate (0 to 10% by weight), benzyl benzoate (0 to 2% by weight), and may be prepared and used as an anticancer composition containing the above components.

The term "pelargonic acid" in the present invention means a saturated fatty acid having 9 carbon atoms. Specifically, in the present invention, the pelargonic acid was extracted from rapeseed to obtain a germicidal composition through a natural component. Generally, the pelargonic acid is used as a component of a food contact surface for sanitization in food handling facilities. The US Food and Drug Administration (FDA) permits the use of synthetic flair (21 CFR 172.515) or pelargonium as an adjuvant and disinfectant for the use of food contact materials, alkaline peeling of fruit or vegetables.

The term " plant fungal diseases "of the present invention means a plant disease caused by fungi. Specifically, mildew disease, gray mold disease, or leaf mold disease are included in fungal diseases. But is not limited to. Plant fungal pathogens are in the form of conidia and are transmitted to other plants through the air. More than 10,000 species of mold are known to cause plant diseases. Specifically, there are a variety of pathogens belonging to Erysiphe , Microsphaera , Podosphaera , Phillactinia, Sphaerotheca , Uncinula , But is not limited thereto.

In the present invention, the plant to be treated with the natural fungicidal composition for controlling the fungal disease of the present invention, which is caused by the occurrence of the fungal disease, is not particularly limited, but examples thereof include cereals such as wheat, barley, Or fruits such as paprika, eggplant, cucumber, onion, poultry, melon, watermelon, tomato, melon and pumpkin or fruits such as strawberries, grapes, apples, pears and blueberries or leaves such as roses, chrysanthemums, begonia and lilac , Hornchon Namcheon, and Gugija.

The term "control" of the present invention means preventing plants such as crops from damage from pests and diseases, elimination of pathogens that have occurred, and inhibition of the spread of pathogens. Methods for creating an inappropriate environment for a pathogen by using a chemical control using a fungicide or insecticide which is toxic to a pest, as well as cultivation and rotation of a resistant plant, a method for removing pathogens by heat treating the plant or treating the soil with steam , A method of attracting and capturing pests using an attractant or sex pheromone, and the like.

The term "composition for controlling fungal diseases" of the present invention means a composition having the effect of preventing the onset of fungal diseases or treating the fungal diseases, including the fungal oils and pelargonic acid.

In the present invention, the content ratio of the clove oil and the pelargonic acid contained in the composition is not particularly limited but may be, for example, 3: 1 to 5: 1 (w / w) (w / w).

In addition, cloven oils and pelargonic acid provided by the present invention are insoluble in cold water, clove oil forms an oily phase, and pelargonic acid also forms an oily phase. Therefore, they are suitably mixed to prepare a composition for controlling powdery mildew For the purpose, the composition for controlling powdery mildew disease may further comprise a surfactant.

The surfactant may exhibit an effect of improving the mixing and dispersing properties of the clodream oil and the pelargonic acid. The content of the surfactant is not particularly limited, but is, for example, 30 to 50% by weight based on the final weight of the composition . When the content of the surfactant is less than 30% by weight, the mixing and dispersing degree of the clodream oil and the pelargonic acid may be lowered, and as a result, the controlling effect may be decreased. When the content of the surfactant exceeds 50% by weight, The content of clove and pelargonic acid is relatively decreased, and the effect of controlling can be reduced.

Examples of the surfactant include, but are not limited to, Tween-20 (Polyoxyethylene sorbitan monopalmitate), Tween-40 (Polyoxyethylene sorbitan monopalmitate), Tween-60 (Polyoxyethylene sorbitan monostearate ), Tween-80 (Polyoxyethylene sorbitan monooleate) or quaternary ammonium salt, etc., may be used singly or in combination.

When the surfactant is used, a co-surfactant may be further used. As the co-surfactant, a polymer such as polyethylene glycol may be used. The mixing ratio of the surfactant and the co-surfactant may be, for example, 3: 1 to 4: 1 (w / w).

Further, the surfactant may be heated and mixed with the clotting oil and / or the pelargonic acid at a temperature of 20 to 60 ° C, and if necessary, 5 to 40% by weight of water, methanol or ethanol as a solvent, , Specifically 10 to 20% by weight, based on the total weight of the composition.

According to one embodiment of the present invention, a natural fungicidal composition was prepared by mixing clove oil and pelargonic acid with a surfactant Tween-20, and the mixture of clods oil alone or pelargonic acid alone was mixed with a surfactant, (Example 1). As a result of spraying the above-prepared natural fungicidal mixture composition on a zucchini with mildew disease, it was confirmed that the fungicide exhibited the same level of control effect as that of spraying with a commonly used chemical pesticide, bitteranol 25WP, (Fig. 1 and Table 1). ≪ tb > < TABLE >

According to another embodiment of the present invention, it was confirmed that the natural fungicidal mixture composition was sprayed on the red bean flour produced with mildew disease, and showed the same level of control effect as that of pentiophilad 20EC, which is a commonly used chemical pesticide 2 and Table 2).

According to another embodiment of the present invention, it was confirmed that the natural fungicidal mixture composition was sprayed on sesame seeds with mildew disease, and showed the same level of control effect as that of pentylopyrid 20EC, a commonly used chemical pesticide 3 and Table 3).

According to another embodiment of the present invention, when the natural fungicidal composition is sprayed on the strawberry in which the gray mold fungus has been formed, it shows the same level of control effect as carbendazim 60WP, which is a commonly used chemical pesticide, Pelargonic acid alone showed significantly higher control effects than the single composition of pelargonic acid (FIG. 4 and Table 4).

According to another embodiment of the present invention, the natural fungicidal mixture composition is sprayed on tomatoes with foliar fungus. As a result, the fungicidal composition exhibits the same level of control effect as that of propinve 70WP, which is a commonly used chemical pesticide, Or pelargonic acid alone (Figure 5 and Table 5). ≪ tb >< TABLE >

The composition for controlling the present invention may further contain a substance contained in a commonly used insecticide or bactericide. In addition to the active ingredient, a pharmaceutically acceptable solid carrier, a liquid carrier, a liquid diluent, a liquefied gaseous diluent, A solid diluent or other adjuvant such as an emulsifier, a dispersing agent or a surfactant such as a foaming agent. The controlling composition of the present invention may be formulated into an agricultural composition by mixing the active ingredient with the above excipient. The composition may be formulated into an agricultural composition, and any methods conventionally used in the art may be applied.

In addition, the composition for prevention of the present invention may be provided in the form of a wettable powder, a granule, a powder, an oil, a spray, a film, a capsule or a gel, and is provided with a contact agent of a formulation such as a donut type .

In another aspect, the present invention provides a pesticide formulation for controlling plant fungal diseases comprising the composition.

The term "pesticide" of the present invention means a medicament used for protection and growth of crops. Specifically, the present invention relates to a growth regulator for use in enhancing or suppressing physiological functions of crops as well as agents for use in controlling diseases, insects, weeds, germs, nematodes and mites occurring during cultivation and storage of crops, May be included.

In another aspect, the present invention provides a fertilizer for controlling a plant mold disease comprising the composition.

The term "fertilizer" of the present invention is also referred to as manure, which means nutrients that fertilize land and promote the growth of vegetation. The fertilizer containing the composition of the present invention can be easily used by mixing with the soil before sowing, and can be applied when spraying pesticides and the like.

In particular, the composition of the present invention is an environmentally friendly composition containing components derived from natural products, and is harmless to human beings, livestock and soil, so that it is possible to prevent the disease from occurring more safely than chemical fertilizers. The crop to which the fertilizer of the present invention can be used is not particularly limited as long as it is a plant in which a plant fungal disease occurs.

In another aspect, the present invention provides a method for controlling a plant fungal disease, comprising the step of treating the plant for preventing plant fungal disease.

In the present invention, the treatment method is not particularly limited as long as it exhibits an effect of treating, alleviating or ameliorating a plant fungal disease. However, it is preferable that the control composition is sprayed on a plant having a fungal disease, Or a method of admixing the soil to which the crop is grown can be used.

The composition for controlling the present invention not only exhibits an excellent plant fungal disease control effect but also contains components isolated from natural products and has no side effects and thus can be widely used for the safe and effective control of plant fungal diseases.

1 is a photograph showing the control effect of a natural fungicidal blend composition containing clods oil and pelargonic acid against powdery mildew powder of zucchini, wherein a is a non-treated, b is a 500-fold dilution of a natural fungicidal mixture composition containing clove oil and pelargonic acid d is a chemical pesticide propine 70WP, e is a 500-fold dilution of the clove oil alone composition, f is a 1,000-fold dilution of the clove oil single composition, g is the dilution of the natural fungicidal composition, Represents a 500-fold dilution of a single composition of pelargonic acid, and h represents a 1,000-fold dilution of a single composition of pelargonic acid, which is treated with zucchini with mildew disease.
Fig. 2 is a photograph showing the control effect of a natural fungicidal blend composition containing clods oil and pelargonic acid against red powdery mildew of red bean powder, wherein a is the ratio before the treatment of the natural bactericidal composition, b after treatment, .
Fig. 3 is a photograph showing the control effect of a natural fungicidal mixture composition containing sesame oil and pelargonic acid against sesame powdery mildew, in which a represents the ratio before the treatment of the natural fungicidal composition, b after the treatment, .
Fig. 4 is a photograph showing the effect of controlling a natural fungicidal blend composition containing clods oil and pelargonic acid against a strawberry gray mold, wherein a represents a non-treated state, b represents a natural bactericidal mixed composition containing clover oil and pelargonic acid D is a chemical pesticide propine 70WP, e is a 500-fold dilution of the clove oil single composition, f is a 1,000-fold dilution of the clove oil single composition, c is a 1,000-fold dilution of the natural fungicidal mixture composition including clary oil and pelargonic acid, , g is the 500-fold dilution of the pelargonic acid single composition, and h is the 1000-fold dilution of the single composition of pelargonic acid, treated with strawberry with a gray mold disease.
FIG. 5 is a photograph showing the control effect of a natural fungicidal composition containing clods oil and pelargonic acid against a tomato leaf mold fungus, wherein a is a non-treated, b is 500 times of a natural fungicidal composition containing clove oil and pelargonic acid D is a chemical pesticide propine 70WP, e is a 500-fold dilution of the clove-oil alone composition, f is a 1,000-fold dilution of the clove oil single composition, g is a dilution of the natural germicidal composition including germicidal oil and pelargonic acid, Shows a 500-fold dilution of a single composition of pelargonic acid, and h indicates a result of treating a 1,000-fold dilution of a composition of pelargonic acid alone with tomato foliar disease.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following examples are for illustrative purposes only and are not intended to limit the scope of the present invention.

Example  One. Clove oil  And Pelar Kunshan  Preparation of natural germicidal compositions and diluting solutions containing

To prepare a natural germicidal blend composition comprising clove oil and pelargonic acid, 40 wt.% Clot oil, 10 wt.% Pelargonic acid and 50 wt.% Surfactant Tween-20 were charged to the reactor and mixed for 1 hour Respectively.

 In addition, 40% by weight of clove oil and 60% by weight of surfactant Tween-20 were mixed in the reactor for 1 hour in order to prepare the clove oil single composition, and 10% by weight of pelargonic acid and A natural germicidal composition was prepared by mixing 90 wt% of the surfactant Tween-20 in the reactor for 1 hour.

The prepared germicidal compositions were diluted with water to prepare 500-fold and 1,000-fold dilutions, respectively.

Example  2. Clove oil  And Pelar Kunshan  Analysis of Control Effect on Natural Powdery Mildew Composition Containing Powdery Mildew

Example  2-1. Facility at Zoo  Effect of powdery mildew control

Facility of Natural Fungicidal Mixture Composition Comprising Clodile Oil and Pelargonic Acid To confirm the effect of controlling the powdery mildew on the greenhouse of Zucchini (Sarron), the natural fungicidal composition was sprayed on the facility zucchini (Saron) Respectively.

First, the natural disinfecting composition was sprayed three times at intervals of 7 days in the early stage of powdery mildew from February 20, 2013 to March 3, 2015. On the 15th day after spraying, the leaves of the zucchini plants in which the respective compositions were sprayed on the 15th day were observed (Fig. 1), and the lesion area ratio of 15 leaves totaling 15 weeks by treatment was 0 for no onset, 1 for few onset, In case 2, in many cases 3, in case of high severity, the incidence index of case 4 was examined, and the control value obtained by the following formula is shown in Table 1 below. At this time, as a control group, a facility zucchini (Saran) sprayed with a chemical pesticide (25 WP of bitteranol) 1,000 times was used.

Incidence (%) = Σ (onset number × coefficient) / 4N × 100

Effect of treatment materials on the powdery mildew powdery mildew Treated material Dilution factor Outbreak rate (%) Average Significant difference
DMRT 5% The control
(%) 1r 2r 3r Clover (40%) +
Pelar Kunshan (10%) 500X 2.2 2.5 3.2 2.6 g 88.4 1,000X 4.1 3.8 3.6 3.8 f 83.0 Clover (40%) 500X 5.8 6.4 6.8 6.3 e 71.9 1,000X 9.8 11.2 11.1 10.7 c 52.2 Pelar Kunshan
(10%) 500X 7.4 8.6 7.8 7.9 d 64.7 1,000X 14.2 13.6 14.0 13.9 b 37.9 Bitteranol 25WP 1,000X 2.4 2.5 2.4 2.4 g 89.2 No treatment - 19.1 25.3 22.9 22.4 a -

As shown in the above Table 1, when the respective treatment materials were diluted by 1,000 times and sprayed, the mixed natural disinfecting composition containing clods oil and pelargonic acid showed a controlling effect of 83.0%, and clenching oil alone (52.2%) or Pelar (37.9%) of Kunshan alone. In addition, it was confirmed that even when the treated materials were diluted by 500 times and sprayed, the mixed natural disinfecting composition including clary oil and pelargonic acid exhibited a higher control value than the single composition.

In addition, it was confirmed that the mixed natural germicidal composition exhibited a control effect similar to that of the chemical agricultural chemical bitteranol 25WP used as the comparative agent.

1 is a photograph showing the control effect of a natural fungicidal mixture composition containing clods oil and pelargonic acid against powdery mildew powder of zucchini, wherein 500 g of a natural fungicidal mixed composition comprising a non-treated, a bermudate and pelargonic acid, D is a chemical pesticide propine 70WP, e is a 500-fold dilution of the clove oil single composition, f is a 1,000-fold dilution of the clove oil single composition, c is a 1,000-fold dilution of the natural fungicidal mixture composition including clary oil and pelargonic acid, , g is a 500-fold dilution of the pelargonic acid single composition, and h is the 1,000-fold dilution of the single composition of pelargonic acid treated with zucchini with mildew disease. As shown in Fig. 1, when a natural fungicidal mixed composition containing clove oil and pelargonic acid was sprayed (in comparison with the composition of g) and the composition of pelargonic acid alone (g and h of Fig. 1) 1 (b) and (c)), it was found that the onset of powdery mildew was greatly suppressed and controlled. In addition, when left without spraying the natural germicidal composition, damage of powdery mildew was observed throughout the zucchini leaves (Fig. 1 (a)).

Example  2-2. Effect of controlling powdery mildew on red bean cultivar

In order to confirm the effect of controlling the powdery mildew on the non-cultivated red bean (Hong Kong) of natural fungicidal mixture composition containing clods oil and pelargonic acid, the natural fungicidal composition was sprayed on the non-cultivated red bean The control value was calculated.

First, in the early days of powdery mildew, the plants were sprayed twice at intervals of 7 days (August 29 and September 5), and at 30 weeks per treatment, except for the top two branches per week, (FIG. 3), and the control value was calculated in terms of the onset index in the same manner as in Example 2-1. The results are shown in Table 2 below. At this time, as a control group, pentiophilad 20EC, a commonly used insecticide, was diluted to 2,000 and 4,000 times, respectively, and sprayed on a non-cultivated red bean (Hongyan).

Effect of treatment materials on powdery mildew powder Treated material Dilution factor Outbreak rate (%) Average Significant difference
(DMRT 5%) The control
(%) 1r 2r 3r Clover (40%) +
Pelar Kunshan (10%) 500 6.5 5.9 6.3 6.2 d 83.1 1,000 7.3 6.9 6.9 7.0 c 80.9 Pentyopyr rad
20EC 2,000 5.5 5.6 5.9 5.7 d 84.5 4,000 8.8 9.1 9.1 9.0 b 75.5 No treatment - 37.6 36.7 35.9 36.7 a -

As shown in the above Table 2, in the control test for red powdery mildew disease, the natural fungicidal composition including clods oil and pelargonic acid showed a control effect of 80% or more in both the 500-fold diluted solution and the 1,000-fold diluted solution, 20EC diluted solution was diluted to 2,000 times and 4,000 times, respectively.

Fig. 2 is a photograph showing the control effect of a natural fungicidal blend composition containing clods oil and pelargonic acid against red powdery mildew of red bean powder, wherein a is the ratio before the treatment, b after treatment and c after treatment Lt; / RTI > As shown in Fig. 2, when the natural fungicidal composition of the present invention was sprayed on the powdery mildew powder of red bean powder, the occurrence of powdery mildew was significantly suppressed and controlled after spraying (Fig. 2 (b) Could know. In addition, when the natural bactericidal composition was allowed to stand without spraying, spreading damage of powdery mildew was observed throughout the whole of the red bean plant (Fig. 2 (c)).

Example  2-3. Control of powdery mildew on cultivated sesame (peace) plant

In order to confirm the effect of controlling the powdery mildew on the cultivation of the house-grown sesame seeds, the natural pasteurization composition was sprayed on the sesame seedlings grown in the facility house of Miryang National Fisheries Research Institute in 2014 The control value was calculated.

First, spraying was carried out twice at intervals of 7 days (August 29 and September 5) at the beginning of powdery mildew. After spraying of the natural disinfecting composition, the area ratio of powdery mildew disease to 10 leaves from the top five branches per week was checked at 30 weeks per treatment (Fig. 3), and converted into the onset index in the same manner as in Example 2-1 The results are shown in Table 3 below. At this time, as a control, sesame (cultivated in house) cultivated by diluting and spraying pentiofurad 20EC was used.

Effect of Treated Material on Powdery Mildew of Sesame Treated material Dilution factor Outbreak rate (%) Average Significant difference
(DMRT 5%) The control
(%) 1r 2r 3r Clover (40%) +
Pelar Kunshan (10%) 500 12.2 13.1 13.3 12.9 d 85.4 1,000 15.4 15.5 16.0 15.6 c 82.4 Pentyopyr rad
20EC 2,000 10.8 11.4 11.5 11.2 e 87.3 4,000 20.3 20.3 20.1 20.3 b 77.0 No treatment - 88.1 88.8 88.3 88.4 a -

As shown in the above Table 3, in the control test for the sesame powdery mildew of the mixed composition including clodream oil and pelargonic acid, the natural fungicidal mixture composition showed a control effect of 80% or more in both the 500-fold diluted solution and the 1,000-fold diluted solution And it was confirmed that the control effect was the same as that obtained when the dilution solution of pentiofuril 20EC was diluted to 2,000 times and 4,000 times, respectively.

Fig. 3 is a photograph showing the effect of controlling the natural fungicidal mixture composition containing sesame oil and pelargonic acid against sesame powdery mildew, wherein a is the ratio before the treatment of the natural fungicidal composition, b after the treatment, Results are shown. As shown in FIG. 3, when the natural fungicidal composition of the present invention was sprayed on sesame powdery mildew, the incidence of powdery mildew was significantly suppressed after spraying (FIG. 3 (b) . In addition, when the natural bactericidal composition was left without being sprayed, spreading damage of powdery mildew was observed throughout sesame plants (Fig. 3 (c)).

Example  3. Clove oil  And Pelar Kunshan  Analysis of the Control Effect on the Gray Mold Fungi of Natural Fungicidal Composition Containing

Example  3-1. Facilities Strawberry  Effect of Gray Mold Fungus Control

Facility of Natural Fungicidal Compound Composition Comprising Clodile Oil and Pelargonic Acid In order to examine the effect of controlling the gray mold fungus on the strawberry, the natural fungicidal composition was sprayed on the strawberry of the Kyeongsangbuk-do, Respectively.

First, the natural germicidal composition was sprayed three times at intervals of 7 days in the early days when gray mold disease occurred from January 13, 2015 to February 26, 2015 (FIG. 4). On the 15th day after spraying, the control of the natural germicidal composition was determined by counting the numbers of the onset flower buds and the fruit per 30 weeks of treatment, and the results are shown in Table 4 below.

Effect of controlling treatment materials on strawberry mold gray mold Treated material Dilution factor Onset and number (No.) Average Significant difference
DMRT 5% The control
(%) 1r 2r 3r Clover (40%) +
Pelar Kunshan (10%) 500X 6 6 7 6.3 f 88.3 1,000X 11 9 10 10.0 e 81.5 Clover (40%) 500X 14 12 12 12.7 e 76.5 1,000X 20 24 21 21.7 c 59.8 Pelar Kunshan
(10%) 500X 16 18 14 16.0 d 70.4 1,000X 25 28 26 26.3 b 51.3 Carbendazim 60WP 1,000X 7 5 9 7.0 f 87.0 No treatment - 58 49 55 54.0 a -

As shown in the above Table 4, when the treated material was diluted 1,000 times with respect to the control of the strawberry gray mold, the natural fungicidal composition including clods oil and pelargonic acid showed a control effect of 88.0% (59.8%) or pelargonic acid alone (51.3%), respectively. It was confirmed that the natural fungicidal mixture composition containing the clods oil and the pelargonic acid had a higher control value than that of the single composition even when the respective treatment materials were diluted 500 times and sprayed. In addition, it was confirmed that the above-mentioned natural fungicidal mixture composition showed a similar level of control effect to that of the chemical agrochemical Carbendazim 60WP used as the comparative agent.

FIG. 4 is a photograph showing the control effect of a natural fungicidal blend composition containing clove oil and pelargonic acid against a strawberry gray mold, wherein a is a non-treated, b is a natural fungicidal mixture comprising clove oil and pelargonic acid D is a chemical pesticide propine 70WP, e is a 500-fold dilution of the clove oil single composition, f is 1,000 of the clove oil single composition, c is a 1,000-fold dilution of the natural fungicidal mixture composition including clove oil and pelargonic acid, G is the 500-fold dilution of the pelargonic acid single composition, and h is the 1,000-fold dilution of the single composition of pelargonic acid treated with the strawberry with the gray mold disease. As shown in Fig. 4, when the natural fungicidal blend composition containing the clary oil and the pelargonic acid was sprayed (Fig. 4 (e) and (f)) or pelargonic acid alone 4, b and c), the occurrence of gray mold was greatly suppressed and controlled. In addition, when left without spraying the natural germicidal composition, damage of powdery mildew was observed throughout the whole of the strawberry flower bud and the fruit (Fig. 4 (a)).

Example  4. Clove oil  And Pelar Kunshan  Of the natural germicidal composition In leaf mold mold  Analysis of Control Effect

Example  4-1. Facilities on tomatoes (Super Sun Rod)  About Leaf mold  Control effect

In order to confirm the effect of controlling the foliar fungus on the facility tomato (Super Sunrod), the above-described natural germicidal composition was applied to a tomato (super-sunrode) of 2 bases in Gangseo-gu, Busan, And the control value was calculated.

First, the natural germicidal composition was sprayed three times at an interval of 7 days in the early stage when a leaf mold disease occurred from February 21, 2015 to April 1, 2015 (FIG. 5). The percentage of lesion area of 15 leaves of 5 weeks by treatment was calculated in terms of onset index by the same method as in Example 2-1, and the results are shown in Table 5 below.

Effect of treatment materials on mature tomato leaf mold Treated material Dilution factor Outbreak rate (%) Average Significant difference
DMRT 5% The control
(%) 1r 2r 3r Clover (40%) +
Pelar Kunshan (10%) 500X 7.1 7.8 7.6 7.5 d 85.1 1,000X 9.5 9.8 9.3 9.5 d 81.1 Clover (40%) 500X 10.6 10.9 9.8 10.4 c 79.3 1,000X 14.2 13.6 15.4 14.4 b 71.3 Pelar Kunshan
(10%) 500X 10.4 12.1 12.4 11.6 c 76.9 1,000X 16.8 15.2 16.1 16.0 b 68.1 Propyne 70WP 500X 8.9 9.3 9.1 9.1 d 81.8 No treatment - 43.6 48.3 58.7 50.2 a -

As shown in Table 5, when the treated material was diluted 1,000 times with respect to the tomato leaf mold fungus, the natural fungicidal composition containing clods oil and pelargonic acid showed a control effect of 81.1% , Croton oil alone (71.3%) or pelargonic acid alone (68.1%). It was confirmed that the natural fungicidal mixed composition containing clodjy oil and pelargonic acid had a higher control effect than that of the single composition even when the treated material was diluted 500 times and sprayed. In addition, it was confirmed that the natural fungicidal mixture composition exhibits a control effect similar to that of the chemical pesticide propinev 70WP used as the comparative agent (Table 5).

In addition, FIG. 5 is a photograph showing the control effect of a natural fungicidal blend composition containing clove oil and pelargonic acid against a tomato leaf mold disease, wherein a is a non-treated, b is a natural fungicidal composition containing clove oil and pelargonic acid D is a chemical pesticide propine 70WP, e is a 500-fold dilution of a clove oil single composition, f is a 1,000-fold dilution of a clove oil single composition, and c is a 1,000-fold dilution of a natural disinfecting composition including clove oil and pelargonic acid. , g is the 500-fold dilution of the pelargonic acid single composition, and h is the 1,000-fold dilution of the pelargonic acid single composition, treated with tomato foliar-infested tomato. As shown in Fig. 5, when a natural fungicidal mixture composition containing clove oil and pelargonic acid was sprayed (Fig. 5E and 5F) or pelargonic acid alone (g and h of Fig. 5) 5, b and c), the occurrence of gray mold was greatly suppressed and controlled. In addition, when left without spraying the natural germicidal composition, the spreading damage of the gray mold was observed throughout tomato plants (Fig. 5a).

From the above description, it will be understood by those skilled in the art that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. In this regard, it should be understood that the above-described embodiments are to be considered in all respects as illustrative and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention without departing from the scope of the present invention as defined by the appended claims.

Claims (14)

A natural germicidal composition for controlling a plant fungal disease selected from the group consisting of powdery mildew, gray mold fungus and foliar fungal disease, including clove oil and pelargonic acid,
Wherein the content ratio of the clove oil to the pelargonic acid is 3: 1 to 5: 1 (w / w).
The method according to claim 1,
Wherein the clove oil is volatile oil derived from Eugenia Caryophyllata.
The method according to claim 1,
Wherein the pelargonic acid is a compound derived from rape seed extract.
delete The method according to claim 1,
Wherein the composition further comprises a surfactant.
delete The method according to claim 1,
Wherein the natural germicidal composition exhibits an effect of controlling any one of the plant fungal diseases selected from the group consisting of cereals, vegetables, fruits, flowers and trees.
A pesticide preparation for controlling a plant fungal disease selected from the group consisting of powdery mildew, gray mold and leaf fungal diseases, comprising the composition of any one of claims 1 to 3, 5 and 7.
delete A fertilizer for the control of any one of the fungal diseases selected from the group consisting of powdery mildew, gray mold and leaf fungal diseases, comprising the composition of any one of claims 1 to 3, 5 and 7.
delete A method for treating a plant on which a plant fungal disease selected from the group consisting of powdery mildew, gray mold, and leaf mold disease is treated with the composition of any one of claims 1 to 3, 5, and 7 Wherein the plant fungal disease is a plant disease.
delete 13. The method of claim 12,
Wherein the subject is any one selected from the group consisting of cereals, vegetables, fruits, flowers and trees.

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