JP6034564B2 - ナノ秒パルス電界による、ヒト血小板の活性化および凝集方法ならびに血小板ゲルの形成方法 - Google Patents
ナノ秒パルス電界による、ヒト血小板の活性化および凝集方法ならびに血小板ゲルの形成方法 Download PDFInfo
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- JP6034564B2 JP6034564B2 JP2011536528A JP2011536528A JP6034564B2 JP 6034564 B2 JP6034564 B2 JP 6034564B2 JP 2011536528 A JP2011536528 A JP 2011536528A JP 2011536528 A JP2011536528 A JP 2011536528A JP 6034564 B2 JP6034564 B2 JP 6034564B2
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/14—Blood; Artificial blood
- A61K35/19—Platelets; Megacaryocytes
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- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/40—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing ingredients of undetermined constitution or reaction products thereof, e.g. plant or animal extracts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0057—Ingredients of undetermined constitution or reaction products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
- A61N1/00—Electrotherapy; Circuits therefor
- A61N1/18—Applying electric currents by contact electrodes
- A61N1/32—Applying electric currents by contact electrodes alternating or intermittent currents
- A61N1/326—Applying electric currents by contact electrodes alternating or intermittent currents for promoting growth of cells, e.g. bone cells
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
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Description
本出願は、その全体が参照により本明細書に組み入れられる、2008年11月13日に提出された米国特許仮出願第61/114,363号に対する優先権を主張する。
電界は多様な方法において細胞機能を操作するために用いられうる。電界によって影響を受けうる1つの特定の細胞機構は、細胞内のカルシウム動員である。重要な細胞機能であるカルシウムのシグナル伝達は、多様な細胞応答および作用に関わっている。内部に貯蔵されたカルシウムの放出は、アゴニストに対する応答を促進する、成長および呼吸を活性化する、神経伝達物質の分泌を引き起こす、転写機構を活性化させる、多様なホルモンの放出を引き起こす、筋収縮をもたらす、およびアポトーシス経路における重要な因子の放出を開始することができる(Berridge, M. J., Bootman, M.D., Lipp, P. (1998) Nature. 395, 645-648(非特許文献1))。このカルシウム動員はまた、カルシウムシグナルをさらに伝播させる手段として、および枯渇したカルシウムプールを再度補充する手段として、外部培地から細胞へのカルシウムの流入を誘発する。カルシウムのシグナル伝達を調べるため、カルシウムなどのイオンの動きを操作するために電界を用いることができる。
[本発明1001]
少なくとも約100ピコ秒〜約1マイクロ秒未満の持続時間と、少なくとも約10 kV/cm〜約350 kV/cm未満の電界強度である、少なくとも1回の電気パルスによって活性化された血小板
を含む、血小板ゲル。
[本発明1002]
組織修復マトリクスをさらに含む、本発明1001の血小板ゲル。
[本発明1003]
本発明1001の血小板ゲルを含む包帯。
[本発明1004]
電気パルスが、300 nsの持続時間を有し、かつ電界強度が30 kV/cmである、本発明1001の血小板ゲル。
[本発明1005]
血小板が、少なくとも2回の電気パルス〜10回以下の電気パルスによって活性化される、本発明1001の血小板ゲル。
[本発明1006]
血小板を濃縮する段階;および
該濃縮血小板に少なくとも1回の電気パルスを印加することによって、該濃縮血小板を活性化する段階であって、電気パルスが、少なくとも約100ピコ秒〜約1マイクロ秒未満の持続時間と、少なくとも約10 kV/cm〜約350 kV/cm未満の電界強度とを有する、段階
を含む、血小板ゲルを形成する方法。
[本発明1007]
電気パルスが300 nsの持続時間を有し、かつ電界強度が30 kV/cmである、本発明1006の方法。
[本発明1008]
少なくとも2回の電気パルス〜10回以下の電気パルスを印加する段階をさらに含む、本発明1006の方法。
[本発明1009]
活性化血小板ゲルを創傷に適用する段階をさらに含む、本発明1006の方法。
[本発明1010]
活性化血小板ゲルを心臓組織に適用する段階をさらに含む、本発明1006の方法。
[本発明1011]
血小板を濃縮する段階;
該濃縮血小板に少なくとも1回の電気パルスを印加することによって該濃縮血小板を活性化する段階であって、電気パルスが、少なくとも約100ピコ秒〜約1マイクロ秒未満の持続時間と、少なくとも約10 kV/cm〜約350 kV/cm未満の電界強度とを有する、段階;および
該活性化血小板を心臓組織に適用する段階
を含む、損傷した心臓組織を処置する方法。
[本発明1012]
心臓損傷が心筋梗塞による、本発明1012の方法。
[本発明1013]
心臓組織に再灌流する段階をさらに含む、本発明1011の方法。
[本発明1014]
血小板が自己由来である、本発明1011の方法。
本発明の原理の理解を促進する目的のために、ここで好ましい態様について参照して、その説明のために特定の専門用語を用いる。しかしながら、本発明の範囲のいかなる制限もこれらによって意図されないことが理解されるであろう。むしろ、本発明が関連する技術分野の当業者によって企図されるであろう、本発明のそのような改変およびさらなる修正、ならびに本明細書において例示される本発明の原理のさらなる応用は、本発明の一部であると意図される。
Claims (5)
- 血小板を濃縮する段階;および
該濃縮血小板に少なくとも1回の電気パルスを印加することによって、該濃縮血小板を活性化する段階であって、電気パルスが、少なくとも100ピコ秒であり、かつ、1マイクロ秒未満の持続時間と、少なくとも10 kV/cmであり、かつ、350 kV/cm未満の電界強度とを有する、段階
を含む、活性化された血小板を含む血小板ゲルを含む、損傷した心臓組織を処置するための薬剤を形成する方法。 - 電気パルスが300 nsの持続時間を有し、かつ電界強度が30 kV/cmである、請求項1記載の方法。
- 少なくとも2回の電気パルスであり、かつ、10回以下の電気パルスを印加する段階をさらに含む、請求項1記載の方法。
- 前記血小板が自己由来である、請求項1記載の方法。
- 心臓損傷が心筋梗塞によるものである、請求項1記載の方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US11436308P | 2008-11-13 | 2008-11-13 | |
US61/114,363 | 2008-11-13 | ||
PCT/US2009/064431 WO2010057021A1 (en) | 2008-11-13 | 2009-11-13 | Activation and aggregation of human platelets and formation of platelet gels by nanosecond pulsed electric fields |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2012508771A JP2012508771A (ja) | 2012-04-12 |
JP6034564B2 true JP6034564B2 (ja) | 2016-11-30 |
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Application Number | Title | Priority Date | Filing Date |
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JP2011536528A Expired - Fee Related JP6034564B2 (ja) | 2008-11-13 | 2009-11-13 | ナノ秒パルス電界による、ヒト血小板の活性化および凝集方法ならびに血小板ゲルの形成方法 |
Country Status (6)
Country | Link |
---|---|
US (2) | US20110318319A1 (ja) |
EP (2) | EP2364155B1 (ja) |
JP (1) | JP6034564B2 (ja) |
AU (1) | AU2009313852B2 (ja) |
CA (1) | CA2743841C (ja) |
WO (1) | WO2010057021A1 (ja) |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10912712B2 (en) | 2004-03-25 | 2021-02-09 | The Feinstein Institutes For Medical Research | Treatment of bleeding by non-invasive stimulation |
AR072001A1 (es) | 2008-06-03 | 2010-07-28 | Abbott Lab | Inmunoglobulina con dominio variable dual y usos de la misma |
AU2014275297B2 (en) | 2013-06-03 | 2017-02-16 | Pulse Biosciences, Inc. | Methods and devices for stimulating an immune response using nanosecond pulsed electric fields |
US9238808B2 (en) | 2013-06-06 | 2016-01-19 | General Electric Company | Modular adjustable pulse generator |
US9078862B2 (en) | 2013-06-06 | 2015-07-14 | General Electric Company | Platelet activation using long electric field pulses |
US10421956B2 (en) | 2014-01-17 | 2019-09-24 | General Electric Company | Electric pulse generation systems using capacitive coupling |
US9708597B2 (en) * | 2014-01-17 | 2017-07-18 | General Electric Company | Electric pulse generation systems using capacitive coupling |
US9452199B2 (en) * | 2014-01-17 | 2016-09-27 | General Electric Company | Platelet activation and growth factor release using electric pulses |
US9724155B2 (en) | 2014-12-01 | 2017-08-08 | Pulse Biosciences, Inc. | Nanoelectroablation control and vaccination |
US9752120B2 (en) * | 2015-03-31 | 2017-09-05 | General Electric Company | Activated platelet composition with tunable growth factor level |
US10633645B2 (en) | 2015-12-30 | 2020-04-28 | General Electric Company | Calcium controlled activation of platelets via electrical stimulation |
US10968423B2 (en) | 2018-02-26 | 2021-04-06 | General Electric Company | System and method for electric pulse based activation of biological samples |
US11260229B2 (en) | 2018-09-25 | 2022-03-01 | The Feinstein Institutes For Medical Research | Methods and apparatuses for reducing bleeding via coordinated trigeminal and vagal nerve stimulation |
Family Cites Families (9)
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JPS6353428A (ja) * | 1986-08-25 | 1988-03-07 | Canon Inc | 波面検出素子 |
US6326177B1 (en) | 1999-08-04 | 2001-12-04 | Eastern Virginia Medical School Of The Medical College Of Hampton Roads | Method and apparatus for intracellular electro-manipulation |
JP2004502641A (ja) * | 2000-02-10 | 2004-01-29 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | 治療用血小板及び方法 |
WO2004108208A2 (en) * | 2003-06-06 | 2004-12-16 | Wayne State University, Technology Transfer Office | Methods and apparatus for conversion of eukaryotic cells by application of electric and magnetic fields |
US7565201B2 (en) * | 2004-12-17 | 2009-07-21 | Eastern Virginia Medical School | Activation of calcium-mediated cell functions in cells and tissues, including aggregation of human platelets. by nanosecond pulsed electric fields |
ITRM20040638A1 (it) * | 2004-12-24 | 2005-03-24 | Advance Holdings Ltd | Gel piastrinico semisintetico e metodo per la sua preparazione. |
US20070042016A1 (en) * | 2005-06-23 | 2007-02-22 | Medtronic Vascular, Inc. | Methods and Systems for Treating Injured Cardiac Tissue |
CA2652007C (en) * | 2006-05-12 | 2015-10-13 | Smith & Nephew Plc | Scaffold |
KR20100027091A (ko) * | 2006-11-15 | 2010-03-10 | 코다 테라퓨틱스, 인크. | 상처 치유를 위한 개선 방법 및 조성물 |
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2009
- 2009-11-13 JP JP2011536528A patent/JP6034564B2/ja not_active Expired - Fee Related
- 2009-11-13 EP EP09826853.5A patent/EP2364155B1/en not_active Not-in-force
- 2009-11-13 CA CA2743841A patent/CA2743841C/en active Active
- 2009-11-13 US US13/129,076 patent/US20110318319A1/en not_active Abandoned
- 2009-11-13 AU AU2009313852A patent/AU2009313852B2/en not_active Ceased
- 2009-11-13 EP EP17177585.1A patent/EP3241556B1/en active Active
- 2009-11-13 WO PCT/US2009/064431 patent/WO2010057021A1/en active Application Filing
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2013
- 2013-12-20 US US14/137,444 patent/US20140106430A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
US20140106430A1 (en) | 2014-04-17 |
EP3241556A1 (en) | 2017-11-08 |
AU2009313852A1 (en) | 2010-05-20 |
JP2012508771A (ja) | 2012-04-12 |
EP3241556B1 (en) | 2021-03-10 |
US20110318319A1 (en) | 2011-12-29 |
EP2364155A4 (en) | 2012-12-12 |
CA2743841C (en) | 2020-09-01 |
AU2009313852B2 (en) | 2015-11-26 |
WO2010057021A1 (en) | 2010-05-20 |
CA2743841A1 (en) | 2010-05-20 |
EP2364155B1 (en) | 2017-06-28 |
EP2364155A1 (en) | 2011-09-14 |
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