JP6010434B2 - Body fluid component analyzer and body fluid component analysis method - Google Patents

Description

  The present invention relates to a body fluid component analyzing apparatus and body fluid component analyzing method for analyzing body fluid components of a living body such as glucose, neutral fat, uric acid, cholesterol, and HbA1c.

  As a conventional body fluid component analyzer, an apparatus for analyzing a body fluid component by optically measuring a color reaction occurring in the measurement chamber using a test piece configured to react a body fluid sample with a reagent in the measurement chamber Is known (see Patent Document 1). The body fluid component analyzer according to Patent Document 1 includes a light emitting unit that irradiates light to a measurement chamber of a test piece, a light receiving unit that receives transmitted light that has passed through the measurement chamber, and a calculation unit that calculates absorbance based on the transmitted light. It has.

  The body fluid component analyzer according to Patent Document 1 measures the absorbance at a plurality of points in the measurement chamber, and appropriately selects and averages the absorbance obtained at the plurality of points, for example, when bubbles or the like exist in the measurement chamber However, it is configured to calculate the appropriate absorbance of the measurement chamber and to analyze the body fluid component. Therefore, the light emitting part that irradiates the measurement chamber of the test piece with light at a pinpoint and the light receiving part that receives the transmitted light that has passed through the pinpoint of the measurement chamber are integrated, and moved within the measurement chamber area by driving means. However, it is configured to measure the absorbance at a plurality of points in one measurement chamber by performing measurement. The test piece is provided with a plurality of measurement chambers, and each measurement chamber is configured to measure absorbance at a plurality of points, and a plurality of body fluid components can be analyzed with one test piece.

  However, in the above-described analyzer, in order to calculate the analysis result in one measurement chamber, it is necessary to repeat the slight movement by the driving means and the cycle of irradiation / light reception / calculation a plurality of times. It was difficult to obtain analysis results. For a test piece having a plurality of measurement chambers, each time measurement in one measurement chamber is completed, the light-emitting unit and the light-receiving unit are moved to the next measurement chamber. It was necessary to repeat the calculation cycle, and much time was required until the analysis was completed. In addition, if it is intended to measure the absorbance at a plurality of points, it is necessary to enlarge the measurement chamber to some extent. In the case of a test piece having a plurality of measurement chambers, there is a problem that if the measurement chamber is enlarged, the entire test piece is enlarged and the cost is increased. On the other hand, when the measurement chamber is made small, there is a problem that it is necessary to control a very small moving distance of the driving means.

JP 2010-276444 A

  The problem to be solved by the present invention is to provide a body fluid component analyzer and body fluid component analysis method that can quickly obtain an analysis result, suppress the size of a test piece, and reduce the cost required for the analysis. That is.

The present invention has been made to solve the above-described problems, and the invention according to claim 1 is provided with a plurality of light-transmitting measurement chambers, and does not transmit light in places other than the respective measurement chambers. A test body for a body fluid component analyzer for analyzing a body fluid component by measuring the absorbance of a color reaction between an analyte in a body fluid and a reagent in a plurality of measurement chambers, A light source composed of a light-emitting diode that emits light of a dominant wavelength belonging to the light absorption band of the color reaction that occurs in each measurement chamber, a diffusion plate disposed between the light source and the test piece, and a plurality of the test pieces Including an irradiation unit that irradiates light having a main wavelength to an irradiation range having a two-dimensional spread, and a two-dimensional imaging element in which a plurality of light receiving elements are arranged in a two-dimensional plane, Includes a plurality of measurement chambers and has a two-dimensional extent A light receiving unit that receives light emitted from the light receiving range, a calculation unit that calculates the light intensity obtained for each of the light receiving elements to calculate the absorbance in the measurement chamber, and light that has passed through a normal region of the measurement chamber and a storage unit for storing a normal portion selection rule for selecting only the light receiving element which receives the said calculating means, from the light intensity of said light receiving elements receive the light of previous Symbol receiving range, the measurement chamber A light receiving element outside the boundary and a light receiving element riding on the boundary based on the light passing through and reaching the two-dimensional imaging element of the light receiving unit are determined, and from the light receiving range outside the boundary and on the boundary The light intensity of each of the light receiving elements on the surface is excluded, the light receiving element that completely enters the boundary surrounded by the boundary is selected, and the normality is selected from the light receiving elements that completely enter the boundary surrounded by the boundary. based on the section selection rules The average value of the light intensity in a portion that is-option, a body fluid component analyzer and calculates the absorbance at the measurement chamber.

According to the first aspect of the present invention, it is possible to measure the absorbance of the measurement chamber without moving the irradiation unit and the light receiving unit with respect to the test piece, and to analyze the body fluid component so that the analysis result can be obtained quickly. A possible body fluid component analyzer can be provided.
According to the first aspect of the present invention, it is possible to irradiate light having a predetermined wavelength at low cost, and it is possible to provide a highly accurate body fluid component analyzer with a high SN ratio.
According to the first aspect of the present invention, it is possible to provide a body fluid component analyzer that can simultaneously analyze a plurality of components by calculating absorbance for a plurality of measurement chambers at the same time.
According to the first aspect of the present invention, it is possible to provide a body fluid component analyzer capable of performing an appropriate analysis by calculating the absorbance after excluding an abnormal portion in the measurement chamber.

According to a second aspect of the present invention, the irradiating unit includes a light source composed of a light emitting diode that emits light of a sub wavelength that does not belong to the light absorption band of the color reaction, and the diffusion plate is connected to each of the light sources and the test piece. Disposed in between, and controlled to irradiate the light of the main wavelength and the light of the sub wavelength separately to the irradiation range, and the computing means is surrounded by the boundary based on the normal part selection rule For the light receiving element that completely enters the region, a region where the tendency is reversed between the light intensity of the main wavelength and the light intensity of the sub wavelength is determined as a region where bubbles exist, and the light intensity of the region where bubbles exist excluded are, according to claim 1, wherein the bubble is the average value of the light intensity in the light receiving element falls completely within enclosed by the boundary other than the area which exists, to calculate the absorbance at the measurement chamber This is a body fluid component analyzer.

According to a third aspect of the present invention, the irradiation unit includes a light source that includes a light emitting diode that emits light having a subwavelength that does not belong to the light absorption band of the color reaction, and the diffusion plate is connected to each of the light sources and the test piece. Disposed in between, and controlled to irradiate the light of the main wavelength and the light of the sub wavelength separately to the irradiation range, and the computing means is surrounded by the boundary based on the normal part selection rule For the light receiving element that completely enters the area, the area where the light intensity is reduced at the main wavelength and the sub wavelength is determined as the area where the foreign substance exists, and the light intensity of the area where the foreign object exists is excluded, the average value of the light intensity in the light receiving element falls completely within enclosed by the boundary other than the area where the foreign substance is present, according to claim 1 or claim 2, characterized in that to calculate the absorbance at the measurement chamber Is a body fluid component analyzer

According to the first to third aspects of the invention, it is possible to provide a body fluid component analyzer capable of performing an appropriate analysis by calculating the absorbance after excluding an abnormal part in the measurement chamber. it can.

According to a fourth aspect of the present invention, the storage unit stores a dark value selection rule for selecting a dark value, and the computing means receives the light intensity of the light receiving element outside the boundary where light in the light receiving range is received. Based on the dark value selection rule, an intermediate point between the plurality of measurement chambers is selected as a light intensity that is a dark value, and the light received completely enters the boundary surrounded by the boundary that has received the light in the light receiving range. The humor component analyzing apparatus according to claim 1, wherein the light intensity of the dark value is uniformly subtracted from the light intensity of the element for correction .

According to a fifth aspect of the present invention, the storage unit stores a dark value selection rule for selecting a dark value, and the computing means receives the light intensity of the light receiving element outside the boundary where the light in the light receiving range is received. The light intensity corresponding to the dark value is selected based on the dark value selection rule, and the light intensity corresponding to the dark value is determined from the light intensity of the light receiving element that completely enters the boundary surrounded by the boundary where the light in the light receiving range is received. The body fluid component analyzer according to any one of claims 1 to 3, wherein the correction is performed by subtracting uniformly.

According to the inventions according to claims 4 and 5, it is possible to provide a body fluid component analyzer that can perform dark correction without imaging a test piece a plurality of times and can obtain an appropriate analysis result quickly. it can.

  The invention according to claim 6 is characterized in that a bar code is provided on the test piece, and the light receiving section receives light emitted from the bar code and reads the bar code. The body fluid component analyzer according to any one of the above.

  According to the invention which concerns on Claim 6, while managing the individual | organism | solid of a test piece, the body fluid component analyzer with easy management which identifies the test subject who provided the sample can be provided.

According to a seventh aspect of the present invention, there is provided a body fluid in the plurality of measurement chambers for a test piece provided with a plurality of measurement chambers having light permeability so that light is not transmitted in places other than the measurement chambers. A body fluid component analysis method for analyzing a body fluid component by measuring the absorbance of a color reaction between an analyte in a reagent and a reagent, including a plurality of the measurement chambers of the test piece, and extending in a two-dimensional manner Irradiating light having a main wavelength belonging to the absorption band of the color reaction occurring in each measurement chamber to the irradiation range having light, and light emitted from a light-receiving range including a plurality of the measurement chambers and having a two-dimensional extent The light receiving unit having a two-dimensional imaging element in which a plurality of light receiving elements are arranged in a two-dimensional plane, and the light intensity obtained for each light receiving element is calculated to calculate the absorbance in the measurement chamber. With steps to Calculating the absorbance, from the light intensity of said light receiving elements receiving light of the light receiving range, the boundary based on the light that has reached by passing through the measuring chamber to the two-dimensional image sensor of the light receiving portion The light receiving element outside and the light receiving element riding on the boundary are judged, and the light intensity of each light receiving element riding outside and on the boundary from the light receiving range is excluded and surrounded by the boundary. The body fluid component analysis method is characterized in that the absorbance in the measurement chamber is calculated based on the average value of the light intensity in the light receiving element that completely enters within the range.

  According to the seventh aspect of the present invention, it is possible to analyze the body fluid component so that the absorbance of the measurement chamber can be measured without moving the irradiation unit and the light receiving unit with respect to the test piece, and the analysis result can be obtained quickly. A possible body fluid component analysis method can be provided.

According to an eighth aspect of the present invention, the step of irradiating the light includes a plurality of the measurement chambers of the test piece, and includes an irradiation range having a two-dimensional extension of the light of the main wavelength and the color reaction. The light intensity of the main wavelength and the light intensity of the sub-wavelength for the light-receiving element that irradiates the light of the sub-wavelength not belonging to the light absorption band separately and the step of calculating the absorbance completely falls within the boundary. The area where the tendency is reversed is determined as the area where the bubble exists, and the light intensity of the area where the bubble exists is excluded, and the light transmitted through the normal area of the measurement chamber is received at the boundary. The step of selecting only the light receiving element that completely enters the enclosed area, and the average value of the light intensity of the light receiving element that completely enters the enclosed area other than the area where the bubbles are present, is used to calculate the absorbance in the measurement chamber. The step of calculating A body fluid component analyzing method according to claim 7, characterized in that to obtain.

  According to the eighth aspect of the invention, it is possible to provide a body fluid component analysis method capable of performing an appropriate analysis by calculating the absorbance after excluding an abnormal portion in the measurement chamber.

In the invention according to claim 9, in the step of calculating the absorbance , the intermediate points between the plurality of measurement chambers are darkened from the light intensity of the light receiving element outside the boundary where the light in the light receiving range is received. Selecting as the light intensity value, and correcting by subtracting the light intensity as the dark value uniformly from the light intensity of the light receiving element that completely enters the boundary surrounded by the boundary where the light in the light receiving range is received; and The body fluid component analysis method according to claim 7 or 8 , further comprising:

  According to the ninth aspect of the present invention, it is possible to provide a body fluid component analysis method that can perform dark correction without imaging a test piece a plurality of times and can quickly obtain an appropriate analysis result.

  According to the present invention, it is possible to measure the absorbance of the measurement chamber without moving the irradiation unit and the light receiving unit with respect to the test piece, and to quickly analyze the body fluid component capable of analyzing the body fluid component. Analytical apparatus and methods can be provided.

It is a front view which shows the test piece used when analyzing a bodily fluid component using the bodily fluid component analyzer which concerns on this invention. It is a figure which shows embodiment of the bodily fluid component analyzer which concerns on this invention, Comprising: It is a partial cross section side view in the state which set the test piece. It is a block diagram which shows a function in embodiment of the bodily fluid component analyzer which concerns on this invention. FIG. 4 is a schematic view showing an arrow AA in FIG. 3, showing a two-dimensional image sensor and light that has passed through one measurement chamber of a test piece and arrived. It is a graph showing the light intensity in the light receiving element of 1 row shown by the cross section BB in FIG. FIGS. 5A and 5B are graphs showing light intensity in a row of light receiving elements shown by a cross section BB in FIG. 4, where FIG. 4A shows a case where light having a main wavelength λm is irradiated, and FIG. It is. It is a perspective view which shows the other example of the test piece used when analyzing a bodily fluid component using the bodily fluid component analyzer which concerns on this invention.

  Next, embodiments of the present invention will be described with reference to the drawings. The embodiments described below are preferred embodiments of the present invention, and thus various technically preferable limitations are given. However, the scope of the present invention is particularly limited in the following description. As long as there is no description of the effect, it is not restricted to these aspects.

(Test pieces)
First, the structure of the test piece used with the body fluid component analyzer which concerns on this invention is demonstrated based on FIG. The test piece 10 is configured in the same manner as the test piece described in Patent Document 1, and the supplied body fluid sample is transferred to the measurement chamber to cause a color reaction with the reagent provided in the test piece 10. The color reaction can be measured optically.

  Therefore, the test piece 10 communicates with the supply port 11 through which the bodily fluid sample is supplied, the flow channel 12 serving as a transfer path of the bodily fluid sample, and the flow channel 12, and the bodily fluid sample is transferred to cause a color reaction. It has a measuring chamber 13 that takes place. The body fluid sample is transferred from the supply port 11 to the measurement chamber by an appropriate method such as pressing or suction. Note that a reagent (not shown) that causes a color reaction with a component contained in the body fluid sample is provided between the supply port 11 and the measurement chamber 13.

  The test piece 10 is configured by coupling a plate 14 and a plate 15, the supply port 11 is provided in the plate 14, and the flow path 12 and the measurement chamber 13 are provided in the plate 15. The plate 15 is transparent so as to have light transmission in the place where the measurement chamber 13 is provided so that the measurement chamber 13 can be optically measured, and light is not transmitted in places other than the measurement chamber 13. It is colored black. The test piece 10 shown in FIG. 1 is provided with twelve substantially circular transparent measurement chambers 13 so that twelve different components can be analyzed.

  A bar code 16 is attached to the plate 14 of the test piece 10. The bar code 16 is used to identify 10 test pieces, and the bar code 16 is used to identify the subject who provided the sample. As the barcode 16, either a one-dimensional barcode or a two-dimensional barcode can be used.

(Body fluid component analyzer)
Next, an embodiment of a body fluid component analyzer according to the present invention will be described with reference to FIGS. The body fluid component analyzer 100 is configured to irradiate light toward the measurement chamber 13 of the test piece 10, receive light transmitted through the measurement chamber 13, and obtain the absorbance in the measurement chamber 13 based on the light intensity. The irradiation part 20 which irradiates light to the test piece 10, and the light-receiving part 30 which receives the light emitted from the test piece 10 are provided. Further, as shown in FIG. 3, while functioning as a calculation means for calculating the absorbance based on the light intensity, the CPU 50 for controlling the entire apparatus, the rules for calculating the absorbance, etc. are stored, and the light received by the light receiving unit 30 A storage unit 60 for storing the intensity information and a display unit 70 for displaying the analysis result are provided. In addition, the body fluid component analyzer 100 includes a heater 90 that heats the test piece 10, thereby maintaining an appropriate temperature condition for the reaction.

  The irradiating unit 20 includes a housing 21, and inside thereof, light sources 22, 23, and 24 made of bullet-type LEDs (light emitting diodes) that are closely arranged, and a diffusion plate 25 that is provided apart from each other. , 26. The light emitted from the light sources 22, 23, 24 is configured to reach the test piece 10 via the diffusion plates 25, 26, thereby enabling the test piece 10 to be irradiated with substantially parallel light. The irradiated range has a two-dimensional spread. In addition, by using a bullet-type LED, it is possible to configure the irradiation unit 20 that can irradiate light of a predetermined wavelength at a low cost. The irradiating unit 20 is configured to be capable of irradiating at least two wavelengths of light, and is selected such that the light sources 22 and 23 emit light of the main wavelength λm and the light source 24 emits light of the sub wavelength λs. Then, the light sources 22 and 23 and the light source 24 are controlled to be lit separately. The description of the main wavelength λm and the sub wavelength λs will be described later.

  The light receiving unit 30 is disposed to face the irradiation unit 20 with the test piece 10 interposed therebetween. The light receiving unit 30 includes a two-dimensional imaging element 31 and a lens 32, and is configured to receive (image) light emitted from the two-dimensional light receiving range J. The two-dimensional imaging element 31 receives light emitted from the portion of the test piece 10 that falls within the light receiving range J by imaging one side of the test piece 10 through the lens 32. The light receiving range J captured by the two-dimensional imaging device 31 is a surface opposite to the surface irradiated by the irradiation unit 20 and is configured to supplement light emitted from the irradiation unit 20 and transmitted through the measurement chamber 13. Yes. The two-dimensional image pickup device 31 includes a plurality of light receiving elements arranged in a two-dimensional plane, and detects the light intensity for each light receiving element. As the two-dimensional image sensor 31, a CCD image sensor, a CMOS image sensor, or the like is preferably used.

  Note that the light receiving range J shown in FIG. 2 is set to be narrower than the area of the region in which the measurement chamber 13 is arranged, and the entire measurement chamber 13 of the test piece 10 cannot be covered by one imaging. Therefore, by using the driving means (not shown in FIG. 2), by moving the test piece 10 relative to the light receiving range J (left and right direction in FIG. 2), the region where the measurement chamber 13 is arranged is changed. It is possible to divide and take an image, so that the entire measurement chamber 13 can be covered. The driving means can be constituted by an appropriate device such as a rack and pinion.

  Further, although the barcode 16 is attached to the test piece 10, it can be configured to be read by the two-dimensional imaging device 31. In that case, it is possible to use the above-described driving means that moves the test piece 10 relative to the light receiving range J so that the barcode 16 enters the light receiving range J. In addition, when all the measurement chambers 13 of the test piece 10 and the barcode 16 are in the light receiving range J, no driving means is required.

  Moreover, in the above-mentioned embodiment, although the light source of the irradiation part 20 is comprised from several bullet-type LED, it can replace with this and can also employ | adopt chip | tip LED which irradiates the light of multiple wavelengths. With this configuration, the size of the apparatus can be reduced, and there is no need to finely adjust the direction of light.

  Furthermore, in the above-described embodiment, the irradiation unit 20 is configured to irradiate the test piece 10 with substantially parallel light by using the diffusion plates 25 and 26. The reason is that by irradiating the irradiation region of the irradiation unit 20 with light of uniform light intensity, the SN ratio is uniformly increased for each measurement chamber 13 existing in the irradiation region, and a highly accurate measurement result is obtained. It is in that it can be obtained. Therefore, although it is preferable to use the diffusion plates 25 and 26, it is not essential. When the diffusion plates 25 and 26 are not used, accuracy can be compensated by performing blank correction. In the blank correction, correction information is acquired by receiving light from a dummy test piece simulating the shape of the measurement chamber 13 or by receiving light from the irradiation unit 20 without using any test piece. The analysis result is corrected based on the correction information.

  Next, the exchange of information in the body fluid component analyzer 100 according to the present embodiment will be described based on the block diagram shown in FIG. The body fluid component analyzer 100 calculates the absorbance based on the irradiation unit 20, the light receiving unit 30, the driving unit 40 that moves the light receiving unit 30 relative to the test piece 10, and the light intensity received by the light receiving unit 30. In addition, the main configuration includes a CPU 50 for controlling the entire apparatus, a rule for calculating absorbance, a storage unit 60 for storing light intensity information received by the light receiving unit, and a display unit 70 for displaying analysis results. Element. These elements transmit information via the bus 80.

(Calculation of absorbance)
Here, the procedure for calculating the absorbance of the measurement chamber 13 based on the light intensity received by the light receiving unit 30 will be described with reference to FIG. 4 is an arrow view of the image pickup device viewed from the arrow AA in FIG. 3, and reaches the two-dimensional image pickup device 31 of the light receiving unit 30 through one measurement chamber 13 of the test piece 10. It is a schematic diagram showing light. A circle surrounded by the boundary E represents light that has passed through the measurement chamber 13 and reached the two-dimensional imaging element 31 of the light receiving unit 30. The light receiving element Pi (shown by dark hatching) that is completely within the circle surrounded by the boundary E detects the light transmitted through the measurement chamber 13. As shown in FIG. 4, a number of light receiving elements Pi is for receiving the transmitted light to detect the light intensity at a plurality 箇 plants of the measuring chamber 13 at one time of irradiation, it is possible to obtain an absorbance. And it becomes possible to calculate the appropriate light absorbency of a measurement chamber using the light intensity in these multiple places.

Note that (indicated by thin hatching) light-receiving element Pe riding on the boundary E, because the light is irradiated only on a part of the light receiving element, the light intensity is lower than the light intensity of the light receiving element P i. The light receiving element Po outside the boundary E does not receive light (however, a dark value may be detected as described later). Therefore, in order to calculate an appropriate absorbance in the measurement chamber 13, it is necessary to perform an operation after excluding light received by the light receiving elements Pe and Po.

  A method for excluding light received by the light receiving elements Pe and Po and selecting light received by the light receiving element Pi will be described with reference to FIG. FIG. 5 is a graph showing the light intensity in one row of light receiving elements indicated by a cross section BB in FIG. As shown in FIG. 5, since the light receiving element Pi receives transmitted light, high light intensity is detected. Although the light passing through the measurement chamber 13 is not detected by the light receiving element Po, the dark value is detected. In the light receiving element Pe, the light intensity is a value between Pi and Po. Therefore, the light receiving element that detects the light intensity that deviates from the preset numerical range is determined as the light receiving element Pe or the light receiving element Po, and is excluded from the calculation of the absorbance in the measurement chamber 13. Then, by averaging the light intensity received by the light receiving element Pi, the absorbance of the measurement chamber 13 can be calculated.

  At this time, it is possible to calculate the absorbance of the measurement chamber 13 after performing “dark correction” by subtracting the light intensity as a dark value received by the light receiving element Po from the light intensity received by the light receiving element Pi. Accordingly, it is possible to measure an appropriate absorbance with one imaging without requiring two imaging when the test piece is irradiated and when the light is shielded. For the light intensity that is a dark value, the light intensity in a place where light is not transmitted can be used by an appropriate method such as selecting an intermediate point between the plurality of measurement chambers 13.

  The numerical range set for selecting the light receiving elements Pe and Po to be excluded from the calculation of the absorbance can be defined as an absolute value, and is defined based on the highest light intensity (Li). It is also possible to do. This numerical range is stored in the storage unit 60, and is read out and calculated by the CPU 50. A rule for selecting a dark value for performing dark correction is also stored in the storage unit 60 and is read out by the CPU 50 to perform dark correction.

  In a two-dimensional imaging device such as a CCD image sensor or a CMOS image sensor, noise due to dark current may occur. In order to cancel the noise, after taking an image of the test piece when the test piece is irradiated, take an image of the test piece when the light is shielded, and subtract the image when the light is shielded from the image when the light is irradiated. Thus, dark correction can be performed.

  In addition, when the body fluid component analyzer according to the present embodiment is used, even when bubbles are present in the measurement chamber 13, only the light that has passed through the normal region of the measurement chamber 13 is excluded, excluding the bubbles. Absorbance can be calculated. The method will be described with reference to FIG. FIG. 6 is a graph showing the light intensity in a row of light receiving elements shown in section BB in FIG. Is the case of irradiation. The main wavelength λm is a wavelength belonging to the light absorption band of the color reaction occurring in the measurement chamber 13, and the sub wavelength λs is a wavelength not belonging to the light absorption band of the color reaction. In the region V of FIG. 6, the light intensity at the main wavelength λm is higher than the light intensity Lim around it, while the light intensity at the sub-wavelength λs is lower than the light intensity Lis around it. . This tendency suggests that there are large bubbles in region V. That is, for the main wavelength λm, the amount of transmission increases due to a color loss state in a large bubble, and for the sub-wavelength λs, light is reflected by the inner interface of the large bubble, and the amount of transmission decreases. As described above, in the region where large bubbles are present, the light intensity of the transmitted light tends to be opposite between the main wavelength λm and the sub wavelength λs.

  Therefore, the absorbance of the measurement chamber 13 is calculated by excluding the light intensity in the region V where the bubbles are present. For this purpose, bubbles exist in the region of the light receiving element that detects the light intensity that deviates from the preset numerical range, and in which the tendency is reversed between the main wavelength λm and the subwavelength λs. The region V is determined and excluded from the calculation of the absorbance of the measurement chamber 13. Then, by averaging the light intensities received by the light receiving elements Pi other than the region V, it is possible to calculate the absorbance of the measurement chamber 13. The numerical range for determining the region V can be defined as an absolute value, or can be defined based on the highest level of light intensity (Lim, Lis). The numerical range described above is stored in the storage unit 60 as a normal part selection rule, is read by the CPU 50, and is calculated according to the normal part selection rule.

  In contrast to the case where large bubbles exist, when the tendency of the light intensity distribution matches at the main wavelength λm and the subwavelength λs, that is, the light intensity at both the main wavelength λm and the subwavelength λs in a certain region. Is reduced, it is suggested that there is a foreign substance in the region and light transmission is inhibited. Therefore, the region is excluded from the calculation of the absorbance in the measurement chamber 13 by the same method as that for the region V where large bubbles exist.

  The sub-wavelength λs is not absorbed by the color reaction dye in the measurement chamber, and thus the absorbance in the measurement chamber 13 should be essentially zero. However, in actuality, the transmitted light is reduced due to reflection by small bubbles, and the absorbance is reduced. To increase. The increase in absorbance due to small bubbles also occurs at the dominant wavelength λm. Therefore, by subtracting the absorbance at the sub-wavelength λs from the absorbance at the main wavelength λm, it is possible to calculate the absorbance excluding the increase in absorbance due to small bubbles.

  Unlike the method described in Patent Document 1, the method of calculating the absorbance by excluding the bubble portion using the body fluid component analyzer according to the present invention moves the irradiation unit and the light receiving unit very slightly to perform irradiation / reception. It is not necessary to repeat the light receiving cycle, and the measurement result can be obtained quickly.

  In the above description, the method for obtaining an appropriate absorbance in the measurement chamber has been described. Here, since the light receiving unit uses a two-dimensional image sensor, it is possible to image a plurality of measurement chambers at a time. Therefore, it is possible to simultaneously measure the absorbances of a plurality of measurement chambers, that is, to simultaneously analyze a plurality of components contained in a body fluid sample. Of course, an appropriate correction can be performed to calculate an appropriate absorbance for each measurement chamber.

  If the body fluid component to be analyzed is different in each measurement chamber, the color reaction that occurs is also different. The main wavelength λm and the sub wavelength λs are selected according to the color reaction. Therefore, it is preferable to use a plurality of bullet-type LEDs so that the light source can irradiate light having a plurality of wavelengths so as to cover the color reaction in each measurement chamber. For example, four wavelengths of λ = 450 nm, 570 nm, 630 nm, and 810 nm can be appropriately selected and irradiated as the main wavelength and the sub wavelength for each measurement chamber.

  In the description of the present embodiment, the light receiving elements arranged in a line as shown in FIGS. 5 and 6 are taken up in order to explain the rules for selecting the light receiving elements to be excluded when calculating the absorbance. Actually, since the light receiving elements are two-dimensionally arranged, the light receiving elements to be excluded when calculating the absorbance are selected by comparing the light intensities in the two-dimensional plane.

(Other examples of specimens)
In the above description, the test piece 10 shown in FIG. 1 is configured to have a measurement chamber 13 in which a reagent is placed in advance inside a plate 15 that is formed by stacking. However, the test piece that can be used in the body fluid component analyzer 100 according to the present invention is not limited to this, and for example, a test piece 110 configured as shown in FIG. 7 can be used. The test piece 110 is configured such that a color reagent is generated by adding a liquid reagent to the body fluid sample introduced into the measurement chamber, and the color reaction can be optically measured.

  The entire test piece 110 shown in FIG. 7 is made of a light-transmitting resin, and a measurement chamber 113 is provided therein. A supply port 111 through which a body fluid sample is supplied, a reagent introduction port 117 through which a reagent is introduced, and an air hole 118 through which air in the measurement chamber is extracted are provided. The supply port 111 communicates with the measurement chamber 113 via the flow path 112, and the reagent introduction port 117 and the air hole 118 also communicate with the measurement chamber 113, respectively.

  In order to cause a color reaction in the measurement chamber 113, first, a body fluid sample is supplied to the supply port 111, and the body fluid sample is guided to the measurement chamber 113. Next, a liquid reagent is introduced from the reagent inlet. Then, the body fluid sample and the liquid reagent are mixed and a color reaction is caused in the measurement chamber 113. Since the air in the measurement chamber 113 is discharged from the air hole 118, the body fluid sample and the liquid reagent are introduced into the measurement chamber 113 without resistance. The order in which the body fluid sample and the liquid reagent are introduced into the measurement chamber 113 may be reversed.

  The method of optically measuring the color reaction in the measurement chamber 113 with the body fluid component analyzer 100 according to the present invention is the same as when the test piece 10 is used. That is, instead of the test piece 10 shown in FIG. 2, the test piece 110 may be arranged in the body fluid component analyzer 100 and the absorbance in the measurement chamber 113 may be measured using this.

  Note that the test piece 110 shown in FIG. 7 includes two measurement chambers 113 and can measure two different items of body fluid components. The body fluid sample supplied to the supply port 111 is configured to branch into the two measurement chambers 113 through the flow path 112, but the reagent introduction port 117 communicated with each measurement chamber 113 for the reagent. Thus, different liquid reagents are introduced. Note that the number of measurement chambers 113 can be changed as appropriate.

DESCRIPTION OF SYMBOLS 10 Test piece 16 Barcode 20 Irradiation part 22,23,24 Light source 25 Diffusing plate 30 Light-receiving part 31 Two-dimensional image sensor 50 CPU (calculation means)
60 Storage unit 100 Body fluid component analyzer λm Main wavelength λs Subwavelength

Claims (9)

A plurality of measurement chambers having light transmission properties, and a test piece that is configured to prevent light from being transmitted in a place other than each measurement chamber, the analysis object and the reagent in the body fluid in the plurality of measurement chambers, A body fluid component analyzer for analyzing the body fluid component by measuring the absorbance of the color reaction of
A light source composed of a light emitting diode that emits light having a dominant wavelength belonging to an absorption band of a color reaction that occurs in each measurement chamber, a diffuser plate disposed between the light source and the test piece, An irradiation unit including a plurality of the measurement chambers and irradiating light having a main wavelength to an irradiation range having a two-dimensional spread;
A light receiving unit having a two-dimensional imaging element in which a plurality of light receiving elements are arranged in a two-dimensional plane, and receiving light emitted from a light receiving range including the plurality of measurement chambers and having a two-dimensional extent;
A computing means for computing the light intensity obtained for each of the light receiving elements to calculate the absorbance in the measurement chamber;
A storage unit for storing a normal part selection rule for selecting only a light receiving element that has received light transmitted through a normal region of the measurement chamber;
The computing means is
Out of the light intensity of the light receiving element that has received light in the light receiving range, the light receiving element outside the boundary based on the light that has passed through the measurement chamber and reached the two-dimensional imaging element of the light receiving unit, and the boundary Determine the light receiving element on the
Excluding the light intensity of each of the light receiving elements riding on the boundary outside and on the boundary from the light receiving range, and selecting a light receiving element that completely enters within the boundary ,
Absorbance in the measurement chamber is calculated from an average value of light intensity in a part selected based on the normal part selection rule from among the light receiving elements that are completely enclosed within the boundary. Component analyzer. The irradiation unit is
A light source composed of a light emitting diode that emits light of a subwavelength that does not belong to the light absorption band of the color reaction, the diffuser plate is disposed between the light source and the test piece, and the main wavelength is within the irradiation range. And the sub-wavelength light are controlled separately to be irradiated,
The computing means is
Based on the normal part selection rules,
For a light receiving element that completely enters the boundary surrounded by the boundary, a region where the tendency is reversed between the light intensity of the main wavelength and the light intensity of the sub wavelength is determined as a region where bubbles exist,
Exclude the presence of light intensity of a region of the bubble, the average value of the light intensity in the light receiving element falls completely within enclosed by the boundary other than the area where the bubbles are present, to calculate the absorbance at the measurement chamber The body fluid component analyzer according to claim 1. The irradiation unit is
A light source composed of a light emitting diode that emits light of a subwavelength that does not belong to the light absorption band of the color reaction, the diffuser plate is disposed between the light source and the test piece, and the main wavelength is within the irradiation range. And the sub-wavelength light are controlled separately to be irradiated,
The computing means is
Based on the normal part selection rules,
For the light receiving element completely entering within the boundary, the area where the light intensity is reduced at the main wavelength and the sub wavelength is determined as the area where the foreign matter exists,
Exclude the presence light intensity regions of the foreign substance, the average value of the light intensity in the light receiving element falls completely within enclosed by the boundary other than the area where the foreign substance is present, and calculates the absorbance at the measurement chamber The body fluid component analyzer according to claim 1 or 2, characterized by the above. The storage unit stores a dark value selection rule for selecting a dark value,
Based on the dark value selection rule, light having a dark value at an intermediate point between the plurality of measurement chambers out of the light intensity of the light receiving element outside the boundary that has received the light in the light receiving range. The light intensity selected as the intensity is corrected by uniformly subtracting the light intensity as the dark value from the light intensity of the light receiving element completely entering the boundary surrounded by the boundary where the light in the light receiving range is received. The body fluid component analyzer according to any one of 1 to 3. The storage unit stores a dark value selection rule for selecting a dark value,
The calculation means selects light intensity that is a dark value based on the dark value selection rule from light intensity of the light receiving element outside the boundary that has received light in the light receiving range, and calculates light in the light receiving range. The body fluid component according to claim 1, wherein the light intensity of the dark value is uniformly subtracted from the light intensity of the light receiving element that completely enters within the boundary surrounded by the received light. Analysis equipment. The test piece is provided with a barcode,
The humor component analyzing apparatus according to claim 1, wherein the light receiving unit receives light emitted from the barcode and reads the barcode. A plurality of measurement chambers having light transmission properties, and a test piece that is configured to prevent light from being transmitted in a place other than each measurement chamber, the analysis object and the reagent in the body fluid in the plurality of measurement chambers, A body fluid component analysis method for analyzing the body fluid component by measuring the absorbance of the color reaction of
Irradiating an irradiation range including a plurality of the measurement chambers of the test piece and having a two-dimensional extension with light having a main wavelength belonging to an absorption band of a color reaction that occurs in each measurement chamber;
Receiving light emitted from a light receiving range including a plurality of measurement chambers and having a two-dimensional spread by a light receiving unit having a two-dimensional imaging element in which a plurality of light receiving elements are arranged in a two-dimensional plane;
Calculating the light intensity obtained for each light receiving element and calculating the absorbance in the measurement chamber,
Calculating the absorbance comprises:
Out of the light intensity of the light receiving element that has received light in the light receiving range, the light receiving element outside the boundary based on the light that has passed through the measurement chamber and reached the two-dimensional imaging element of the light receiving unit, and the boundary Determine the light receiving element on the
Excluding the light intensity of each of the light receiving elements riding on and outside the boundary from the light receiving range, the measurement is performed by the average value of the light intensity of the light receiving elements that completely enter the area surrounded by the boundary. Absorbance in the chamber is calculated. A body fluid component analysis method. Irradiating the light comprises:
A plurality of the measurement chambers of the test piece are included, and an irradiation range having a two-dimensional spread is separately irradiated with light of the main wavelength and light of a subwavelength that does not belong to the absorption band of the color reaction,
Calculating the absorbance comprises:
For a light receiving element that completely enters the boundary surrounded by the boundary, a region where the tendency is reversed between the light intensity of the main wavelength and the light intensity of the sub wavelength is determined as a region where bubbles exist,
Excluding the light intensity of the region where the bubbles are present, and selecting only the light receiving element that completely enters the boundary surrounded by the boundary that has received the light transmitted through the normal region of the measurement chamber;
The step of calculating the absorbance in the measurement chamber based on the average value of the light intensity in the light receiving element that completely enters the boundary surrounded by the boundary other than the area where the bubbles exist is provided. Body fluid component analysis method. Calculating the absorbance comprises:
Selecting, as a light intensity that is a dark value, an intermediate point between the plurality of measurement chambers, from among the light intensities of the light receiving elements outside the boundary that has received the light in the light receiving range;
The method further comprises the step of uniformly subtracting and correcting the light intensity, which is the dark value, from the light intensity of the light receiving element that completely enters within the boundary surrounded by the boundary that has received the light in the light receiving range. The body fluid component analysis method according to claim 7 or claim 8.

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