JP5997856B2 - Method for producing oral composition - Google Patents

Description

  The present invention relates to a composition containing a black ginger component.

  Black ginger is called Kaempferia parviflora and has the alias of black turmeric or Krachaidam. It is a kind of plant belonging to the genus Zingiberaceae Kaempferia distributed in Southeast Asia. It is known as a health food in traditional medicine such as Thailand and Laos, and is said to have effects such as energy enhancement and nutrition and tonic.

  In addition to selenium and amino acids, active ingredients contained in black ginger include, for example, curcumin and polyphenols, which are said to have an effect of suppressing arteriosclerosis and the like because they have an anticancer action and an active oxygen removal action. ing. Furthermore, because it contains abundant anthocyanins and anthocyanidins, which are a kind of polyphenols, it has been reported that it is effective in reducing fatigue, visual acuity, and eye strain, and methoxyflavonoids that are also a kind of polyphenols. There is a report that it has a function of suppressing the production of uric acid which causes gout and hyperuricemia (Patent Document 1).

  By the way, in general, some foods and drinks containing functional components useful for the human body are poor in intestinal permeation and do not exhibit their original functions sufficiently. Therefore, there is a demand for substances that safely promote intestinal permeation and absorption of such drugs and functional food ingredients. It is known that even a polyphenol-containing material generally has a very small amount of ingested polyphenol taken into the living body. In particular, the mechanism of absorption of polyphenols into the living body varies depending on the type of polyphenol, and water-soluble polyphenols such as catechin, proanthocyanidins, and quercetin are particularly difficult to absorb from the intestinal tract of humans and animals.

  In order to promote these absorptions, conventionally, for example, proanthocyanidins, anthocyanins, catechins, flavonoids and the like have been proposed in combination with absorption promoters that assist permeation absorption (Patent Documents 2 and 3). In addition, a method for reducing the molecular weight of proanthocyanidins having a large molecular weight to such an extent that they can be easily absorbed from the intestinal tract of a living body is disclosed (Patent Document 4).

JP 2011-236133 A JP 2008-174553 A JP 2006-151922 A JP International Publication No. 2006/090830

  However, even with the above method, the absorbability of polyphenols into the living body has not been sufficient. In addition, since plant-derived polyphenols vary greatly in structure and properties depending on the type of plant, the method for improving absorbency known for other plant-derived polyphenols cannot be directly applied to black ginger. As for the polyphenol contained in the black ginger component, it has not been known what effectively helps the intestinal permeation absorbability.

  An object of this invention is to provide the composition which can absorb the polyphenol contained in a body effectively, even when a black ginger component is ingested orally.

  The inventors of the present invention, by coating a part or all of the surface of the above-mentioned black ginger component-containing core with a coating layer containing fats and oils, is surprisingly included in the black ginger component even when ingested orally. It has been found that the absorbability of polyphenols in the body is increased, and the present invention has been completed.

(1) Production of an oral composition characterized in that the dry powder of black ginger is coated with the coating agent by spraying a coating agent containing rapeseed oil or palm oil onto the dry powder of black ginger. Method.
(2) The dry powder of black ginger extract is coated with the coating agent by spraying the dry powder of black ginger extract with a coating agent containing rapeseed oil or palm oil. A method for producing the composition.

  According to the present invention, even when the black ginger component is orally ingested, in particular, the absorption of the polyphenols contained in the black ginger component into the body is enhanced, and the black ginger component before ingestion is prevented from being oxidized and stored. The stability can also be improved and degeneration due to gastric juice after ingestion can be prevented.

It is a figure which shows the time-dependent change of the amount of polyphenols in the blood after ingesting the composition which concerns on the Example of this invention. It is a figure which shows the time-dependent change of the body weight after ingesting the composition which concerns on the Example of this invention, and a high fat diet. It is a figure which shows the visceral white adipose tissue weight after ingestion of the composition which concerns on the Example of this invention. It is a figure which shows the subcutaneous white adipose tissue weight after ingestion of the composition based on the Example of this invention.

  Hereinafter, embodiments of the present invention will be described.

  The composition of this invention contains the particle | grains containing a black ginger component, and the coating layer containing the fats and oils which coat | covered a part or all of the surface. The composition of the present invention has a high absorbability of black ginger components into the body even when taken orally. Therefore, the action which a black ginger component has can be utilized for the purpose of effects, such as an antioxidant action, a cooling symptom improvement action, a weight gain reduction action, a visceral fat, and a subcutaneous fat weight reduction action.

  Particles containing black ginger components refer to particles containing components derived from black ginger, and if they contain components derived from black ginger and are powdered, granulated, granulated, etc., black ginger There is no particular limitation on the processing method. For example, dry powder of black ginger, powdered black ginger extract, and powder obtained by fractionating components in black ginger by any method are applicable. The particles do not need to be solid, and may be liquids such as liposomes and microcapsules.

  As the dry powder of the above-mentioned black ginger, for example, after washing, the sliced black ginger is dried using the sun or a dryer, and then processed as it is or cut into an appropriate shape or size, and then pulverized. It can be obtained by grinding using an apparatus. As the pulverizer, those usually used can be widely used. For example, a pulverizer composed of a raw material hopper, a pulverizer, a classifier, a product holder and the like can be used.

  As the powdered black ginger extract, for example, the black ginger extract is used as it is or in a concentrated form as a liquid, a concentrate, a paste, or a dried product obtained by drying these. be able to. Drying is performed by a method commonly used by those skilled in the art, such as spray drying, freeze drying, reduced pressure drying, and fluidized drying.

  The black ginger extract is obtained by extracting black ginger or a processed product thereof with a suitable solvent. Examples of the solvent used for extraction include lower alcohols such as ethanol, methanol, isopropanol, and butanol, lower esters such as ethyl acetate and methyl acetate, acetone, and a mixture of these with water. Among them, since the composition of the present invention is supposed to be ingested by humans, it is preferable to use ethanol alone or a mixture with water (so-called hydrous ethanol) or hot water.

  When a mixture is used as the solvent, for example, an acetone / water (2/8 to 8/2, volume ratio) mixture, an ethanol / water (2/8 to 8/2, volume ratio) mixture, or the like can be used. . In the case of ethanol / water, it is preferable to add a solvent having a mass of 2 to 20 times the mass of black ginger rhizome and extract it at room temperature or under heating for about 10 minutes to 48 hours.

  Moreover, it is also possible to obtain the extract by a method such as hot water extraction at a temperature of 95 to 100 ° C. and filtering the extract reaching the maximum concentration, followed by spray drying.

  There are no particular limitations on the extraction method to be used, but it is preferable to perform the extraction method under as mild conditions as possible from the viewpoint of safety and convenience. For example, pulverize, crush or shred the raw material plant part or its dried product, add 2 to 20 times the mass of the solvent, and leave it at 0 ° C. to the reflux temperature of the solvent for 10 minutes to 48 hours. The extraction is performed under any conditions such as stirring or refluxing. After the extraction operation, separation operations such as filtration and centrifugation are performed to remove insoluble matters. An extract is obtained by diluting and concentrating as necessary. Further, the same operation may be repeated for insoluble matter, and the extract may be used in combination with the previous extract. These extracts may be used after further purification by a purification method commonly used by those skilled in the art.

  The particles containing black ginger components include the above-mentioned dry powder of black ginger, powdered black ginger extract, and those obtained by fractionating the components in black ginger by any method. You may use as it is, and after adding a suitable binder, an excipient | filler, etc., you may use what was shape | molded by the granulation method of well-known wet and dry granulation.

  Examples of products obtained by fractionating the components in the black ginger by any method include curcumin, methoxyflavones, and anthocyanidins. These may use 1 type powdered and can also mix and use 2 or more types.

  The particle diameter of the particles containing the black ginger component is not particularly limited, and powders, particles, granules and the like can be appropriately selected according to the purpose. Further, the particle size of the particles containing the black ginger component is not particularly limited, and powders, particles, granules and the like can be appropriately selected according to the purpose.

  Bark, roots, leaves, branches or the like can be used as the use site of black ginger for obtaining particles containing the above-described black ginger component. Among them, preferred is a rhizome.

  Even when ingested orally by coating part or all of the surface of the particles containing the black ginger component with a coating agent containing fats and oils, absorption of polyphenols contained in the black ginger component into the body Increases nature.

  Furthermore, by performing the oil-and-fat coating, oxidation of black ginger components including polyphenols can be prevented, and the storage stability of the product can be improved. In addition, black ginger components are strong enough to withstand extraction with organic solvents and are not easily denatured by exposure to gastric juice, etc. Can do.

  Although the specific example of fats and oils is shown below, it is not limited to these. For example, common plants obtained from soybeans, rice, rapeseed, cacao, eggplant, sesame seeds, sesame seeds, palm, strawberries, peanuts, avocado, kapok, poppy, burdock, wheat, evening primrose, camellia, corn, sunflower, etc. Oils and fats and cured products thereof, and animal fats and oils obtained from cows, milk, pigs, sardines, mackerel, sharks, sesame seeds, troughs, etc., and these fats and oils are used alone or in combination. Can be used.

  Even if phospholipids, sterols, waxes and the like coexist in the coating agent, there is no problem. It is also desirable to use other plasticizers for improving the coating performance of the coating agent. Examples of the plasticizer include medium chain triglyceride, glycerin, free fatty acid, distilled acetic acid monoglyceride and the like.

  The substance used as a plasticizer constituting the coating agent is not particularly limited, and examples thereof include proteins such as zein and gluten, and gelling agents such as agar, gellan gum, and carrageenan. These may be used individually by 1 type, or may be used in combination of 2 or more types. There is no restriction | limiting in particular in the usage-amount of a plasticizer, According to the particle | grains containing the black and white ginger component used as fats and oils or a core material, it can adjust suitably.

  In addition, the coating agent may be mixed even if an excipient coexists. There is no restriction | limiting in particular in the usage-amount of an excipient | filler, It can adjust suitably according to the particle | grains containing the black ginger component used as fats and oils and a core material to be used.

  Coating with a coating agent is not particularly limited, and a known method can be applied. For example, a coating solution is prepared by mixing a fat or oil alone or after mixing the above-mentioned substance exhibiting poor water solubility with the fat and oil, and stirring and dissolving in a suitable solvent at room temperature or while heating. The component-containing core can be sprayed with a known sprayer such as a nozzle or an atomizer. Examples of the solvent at this time include alcohol solutions and acidic solutions such as acetic acid. The amount used is not particularly limited as long as oils and fats or substances showing poor water solubility are dissolved, but a coating solution prepared so that these substances are 5 to 50% by weight can be usually used.

  The coating amount of the coating agent can be appropriately adjusted according to the content of the oil and fat, and is not particularly limited, but is 1 to 50 parts by weight with respect to 100 parts by weight of the particles containing the black ginger component. It is preferable.

  There is no restriction | limiting in particular as an ingestion method of the composition of this invention, an ingestion amount, the frequency | count of ingestion, an ingestion time, and an ingestion object, It can select suitably according to the objective. The intake amount can be appropriately selected in consideration of various factors such as the age, weight and constitution of the individual to be ingested. In addition, the animal species to be ingested are mainly suitably applied to humans, but animals other than humans, such as mice, rats, hamsters, gerbils, etc., as long as their effects are exerted. , Ferrets, rabbits, birds, dogs, cats, sheep, goats, cows, horses, pigs, monkeys, reptiles, and the like.

  The composition of the present invention is mainly used for oral use, and as its form, foods and drinks, preparations and the like can be appropriately selected. As said food-drinks, the said composition may be used as it is, and you may use it melt | dissolving thru | or disperse | distributing only with water (purified water etc.).

  Further, various nutritional ingredients are added within a range not impairing the effect of the composition, and the form suitable for edible use, for example, powder, granule, granule, liquid, paste, cream, tablet, capsule , Caplet, soft capsule, rod, plate, block, gel, jelly, gummy, wafer, biscuit, bowl, chewable, syrup, stick, etc. It can also be provided.

  There is no restriction | limiting in particular as kind of these applicable food-drinks, According to the objective, it can select suitably, For example, drinks, such as a soft drink, a carbonated drink, a nutrition drink, a fruit drink, a lactic acid drink; Ice cream, ice Frozen confectionery such as sorbet and shaved ice; noodles such as buckwheat, udon, harusame, gyoza skin, sushi mai, Chinese noodles and instant noodles; rice cake, candy, gum, chocolate, tablet confectionery, snack confectionery, biscuits, jelly, jam, cream , Baked confectionery, bakery and other confectionery; crab, salmon, clam, tuna, sardine, shrimp, bonito, mackerel, whale, oyster, saury, squid, red scallop, scallop, abalone, sea urchin, crabs, tocobushi, etc .; Processed fishery and livestock products such as ham, sausage; dairy products such as processed milk and fermented milk; salad oil, tempura oil, margarine, mayonnaise , Shortening, whipped cream, dressing and other fats and oils processed foods; sauces, sauces and other seasonings; curry, stew, oyakodon, rice bowl, miscellaneous cooking, Chinese rice bowl, bowl, tempura, eel rice, hayashi rice, oden, mabodorf Retort pouch foods such as beef bowl, meat sauce, egg soup, omelet rice, dumplings, shumai, hamburger, meatballs; various forms of health foods, dietary supplements and the like.

  There is no restriction | limiting in particular as content of the said composition contained in the food / beverage products of this invention, According to the objective, it can select suitably.

  Furthermore, the preparation may contain a pharmacologically acceptable carrier, and examples thereof include tablets, granules, powders, capsules and the like. It is preferable to encapsulate and increase the amount that reaches the internal organs as it is. Furthermore, one agent of the present invention can be supplied in the form of drinks, syrups, and jelly, in addition to those having dosage forms such as tablets, powders, and capsules.

  As a method for producing capsules, a conventionally known method for producing soft capsules may be used except that the composition of the present invention is used as the contents. Examples of such a production method include a method of encapsulating contents with a rotary filling machine using a capsule film sheet and molding a capsule preparation, or a method of producing seamless capsules by a dropping method.

  The tablet can be prepared by a known tableting method by adding an appropriate binder, excipient, disintegrant and, if necessary, a lubricant to the composition of the present invention. As for the granule, known various wet and dry granulation methods can be applied, and it is molded together with an appropriate binder and excipient. Furthermore, drinks, syrups, jellies and the like can be prepared by adding known sugars, acids, fragrances and the like to prepare flavors and preparing them by known production methods.

  The composition of the present invention can be applied without particular limitation as long as it is a use utilizing the effect produced by the active ingredient contained in black ginger. For example, the composition of the present invention is a food, drug or the like mainly used orally for the purpose of anti-oxidation effect, cooling effect improvement effect, weight gain reduction effect, visceral fat and subcutaneous fat weight reduction effect, etc. Can be used.

  Next, the present invention will be described in more detail based on examples, but the present invention is not limited thereto.

(Polyphenol absorption enhancement effect)
The test substance was prepared as follows.

<Example 1>
Dry powder (black ginger powder) of black ginger coated with palm oil was mixed with corn oil to prepare 150 mg / mL, and suspended using vortex.

<Example 2>
A test substance was obtained in the same manner as in Example 1, except that black ginger powder was coated with rapeseed oil.

<Comparative Example 1>
Black ginger powder was mixed with corn oil to prepare 150 mg / mL, and suspended using vortex.

  Using the above test substance, blood was collected 1, 4, and 8 hours after administration of black ginger orally in the following manner (only 1 hour after administration as a blank), and the total polyphenols in the blood were collected. The amount was measured. The result is shown in FIG.

(1) Experimental animals and breeding methods Six-week-old SD male rats were prepared and used for experiments after a habituation period of 5 days or more. The grouping was randomly performed immediately before the test. The feed during the acclimation period was a commercially available MF chow. On the day of the test, the animals were fasted until the end of the test.

(2) Administration method of test substance After fasting for 16 hours or more, the test substance solution was forcibly orally administered with a sonde so as to be 10 mL / kg. Table 1 shows the blood collection time, the test substance, and the number of individuals in each group to which this was administered.

(Serum pretreatment method)
Methanol (5 mL), water (5 mL), and 0.1 mol / L hydrochloric acid (1 mL) were sequentially passed through a Waters solid phase extraction cartridge HLB (60 mg) to prepare a preconditioning. Subsequently, water (1 mL) and 0.1 mol / L hydrochloric acid (1 mL) were added to 1 mL of mouse serum, mixed, passed through the cartridge described above, and the non-adsorbed fraction was discarded. Further, 1.5 mol / L formic acid aqueous solution (2 mL) and methanol aqueous solution (5% by volume) (2 mL) were passed through and washed. Thereafter, 0.1% methanol formate (3 mL) was passed through, and the eluted fraction was collected in a 15 mL centrifuge tube. The obtained fraction was concentrated under reduced pressure overnight with a centrifugal evaporator (without heating) and completely dried, and water (200 μL) was added thereto and dissolved by ultrasonic waves. After centrifugation (15,000 rpm, 5 minutes), the supernatant was collected in a 1.5 mL eppen and used as a sample for measuring the total amount of polyphenols.

(Total polyphenol measurement method)
100 μL of each specimen was measured in a 1.5 mL Eppendorf tube, 10% (w / w) sodium carbonate (100 μL) was added, and the mixture was allowed to stand for 10 minutes. Further, Folin-Ciocalteu reagent (100 μL) was added, and color was allowed to develop for 1 hour at room temperature. After the colored sample was centrifuged (15,000 rpm, 5 minutes), the supernatant (200 μL) was transferred to a 96-weLL microplate, and the absorbance at 730 nm was measured. Catechin monohydrate was used as the standard for quantification. A 250 μg / mL aqueous solution was prepared and diluted appropriately to prepare 125, 100, 75, 50, 25, and 12.5 μg / mL standard solutions. These were processed in the same manner as each sample, and a calibration curve was created from the measurement results. The results were applied to serum sample data and used as quantitative results.

  As is clear from FIG. 1, the amount of polyphenol in the blood of the group ingesting the black ginger bulk powder subjected to the oil-and-fat coating of Examples 1 and 2 is higher than that ingesting the black ginger bulk powder. Is shown. In particular, Example 2, which was coated with rapeseed oil, was found to have a large amount of polyphenol taken into the blood, and it persisted for a long time.

(Reducing body weight gain and reducing body fat weight)
Subsequently, as a specific effect of the absorption enhancement of the polyphenol of the present invention on the living body, attention was paid to the weight gain reduction and body fat weight reduction actions, and the evaluation was performed.

(1) Test substance administration method and evaluation method Four-week-old C57BL / 6J male mice were habituated to MF diet for 5 days. Subsequently, the groups were divided into 3 groups of 6 animals per group so that the average body weight per group was uniform. As shown in Table 2, one of these groups was allowed to freely take only a high fat diet without any addition as a control group, and the other two groups were each represented as a high fat diet. A feed supplemented with 1% of the test substance shown in (1) was ingested freely for 69 days. From the start of free intake of each group, body weight and food intake were measured periodically. In addition, blood collection and autopsy were performed under anesthesia on the 69th day from the start of the test (day 0), and the visceral and subcutaneous white adipose tissue weights were measured.

(2) Test substance Table 3 shows the composition of the high fat diet used in this test. Here, the control group was ingested with the test substance set to 0 and the amount replaced with α-potato starch. The black ginger bulk powder and the black ginger bulk powder coated with rapeseed oil are the same as those used in Example 2 above.

(3) Evaluation result of weight gain reduction action FIG. 2 shows the change in weight of each group. There was no significant difference in intake between each group. Compared to the control, there was a significant difference between the black ginger powder coated with rapeseed oil on the 42nd day (6th week) and the last on the 57th day (8th week). Significant differences were observed. From the above examination, the weight gain reduction action is higher in the order of rapeseed oil coated black ginger bulk powder> black ginger bulk powder.

  Next, FIG. 3 and FIG. 4 show the visceral and subcutaneous white adipose tissue weight of each group. Mice fed with black ginger bulk powder showed a significant reduction in adipose tissue weight compared to controls in visceral and subcutaneous white fat (* and ** are significant at p <0.05 and 0.01, respectively, relative to controls) Indicates that there is a difference.) And the reduction | restoration effect | action of the structure | tissue weight was high in order of the rapeseed oil coat black ginger bulk powder> black ginger bulk powder.

  From the above results, it was found that the black ginger powder coated with the rapeseed oil of the present invention showed higher effects of reducing body weight gain, visceral fat and subcutaneous fat weight than black ginger powder. .

  The above results indicate that the increased absorption of polyphenol by the rapeseed oil coat shown in the previous test appears as a weight gain reducing action.

Then, the example which applied the particle | grains containing the black ginger component of this invention to the oral preparation is shown.
(Manufacture of black ginger juice powder coated product)
After washing 500 g of black ginger, it was crushed and pressed to obtain a juice. After centrifuging and filtering this, the obtained juice was concentrated under reduced pressure to obtain a concentrate. The obtained concentrate was freeze-dried to obtain black ginger juice powder. The obtained black ginger juice powder was coated with rapeseed oil to obtain a black ginger juice powder coated product.

(Manufacture of black ginger extract powder coated product)
After washing 500 g of black ginger, it was dried and pulverized, and extracted with stirring at 1000 mL in ethanol at 25 ° C. for 24 hours. The extract was filtered to obtain a filtrate, and then ethanol was distilled off under reduced pressure to obtain a concentrate. The obtained concentrate was dried by lyophilization to obtain black ginger extract powder. The obtained black ginger extract powder was coated with rapeseed oil to obtain a black ginger extract powder coated product.

<Example 3 (Manufacture of liquid agent)>
Each component was blended at the blending ratio in Table 4 to obtain a liquid.

<Example 4 (Production of granule 1)>
According to Table 5, sucralose 0.5 kg, citric acid 5 kg, reduced maltose starch syrup 64.5 kg, indigestible dextrin 67.2 kg, licorice extract 0.5 kg, black ginger crushed 16.8 kg, cyclodextrin 8.4 kg, ginger yak 5 kg is put into a fluidized bed granulator and mixed by airflow for several minutes. This was granulated by spraying 2000 mL of water for 1 minute. Subsequently, the obtained granulated product was sieved with a 30-mesh sieve to obtain granules.

<Example 5 (Production of granule 2)>
In the same manner as in Example 4, the ingredients shown in Table 6 were blended to prepare granules.

<Example 6 (Production of soft capsule 1)>
The content liquid mix | blended with the mixture ratio of Table 7 was prepared, and it was set as the soft capsule by filling the capsule membrane | film | coat mix | blended with the mixture ratio of Table 8. Encapsulation was performed by casting a capsule coating solution to form a film, filling the content solution inside, heat-sealing, and drying the formed soft capsule.

<Example 7 (Production of soft capsule 2)>
The content liquid blended at the blending ratio shown in Table 9 was prepared, filled into the capsule film blended at the blending ratio shown in Table 10, and soft capsules were obtained in the same manner as in Example 6.

<Examples 8 to 12>
Examples 8 to 12 were prepared in the same manner as in Examples 3 to 7 except that a black ginger extract powder coated product was used instead of the black ginger juice powder coated product.

<Examples 13 to 17>
Examples 13 to 17 were prepared in the same manner as in Examples 3 to 7 except that a dry ginger rhizome dry powder coat product was used instead of the black ginger juice powder coat product.

Claims (2)

  A method for producing an oral composition, wherein the dry powder of black ginger is coated with the coating agent by spraying a coating agent containing rapeseed oil or palm oil onto the dry powder of black ginger.   An oral composition characterized in that the dry powder of black ginger extract is coated with the coating agent by spraying a coating agent containing rapeseed oil or palm oil onto the dry powder of black ginger extract. Production method.