JP5992707B2 - ポリマーバイオプロセス容器とともに用いる複合センサアセンブリ、ポリマーバイオプロセス容器とともに用いる複合アセンブリ、およびセンサまたは複合アセンブリの製造プロセス - Google Patents
ポリマーバイオプロセス容器とともに用いる複合センサアセンブリ、ポリマーバイオプロセス容器とともに用いる複合アセンブリ、およびセンサまたは複合アセンブリの製造プロセス Download PDFInfo
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Classifications
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
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- G01N2021/7786—Fluorescence
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
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- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
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Description
バイオプロセス用の使い捨てシステムの人気の高まりは市場において明らかであり、典型的なバイオテクノロジー製造設備を考えると容易に理解できる。従来のガラス/鋼鉄バイオリアクタ、ミキサ、および浄化システムを用いて設備を実現するのに必要なインフラストラクチャは大型であり、そのようなバイオプロセスシステムを構築するのに必要な時間および費用は多大になり得る。装置自体、ならびに侵入および退出配管の両方が316L電解研磨ステンレス鋼などの不活性材料を利用するという要件のために、多額の初期投資およびバイオリアクタ(すなわちバイオプロセス容器)ミキサおよび下流処理装置(たとえばクロマトグラフィースキッド、濾過システム)が必要であり、これらはすべて大きな占有面積を有する。これとは対照的に、使い捨てプラットフォームのサイズおよび形状因子は一般的に、従来の剛性のガラス/鋼鉄解決策と比べると、保管および再構成が容易である。使い捨てシステムの他の利点として、支持インフラストラクチャの小型化、具体的には準備および滅菌時間の短縮を含む従来の設計に対する時間の節約、実行後の容器洗浄のための浄化水の必要性の減少、ならびに実行後の維持時間の大幅な短縮が挙げられる。また、使い捨てシステムおよびそれらに関連したプラスチック配管は、製造および処理要件が変わるとともに、迅速かつ効率的に再構成および検査されることに適している。本発明の文脈では、主に使い捨てバイオリアクタに焦点を当てるが、本発明の原理は包括的に、上流処理(USP)および下流処理(DSP)領域の両方における、バイオプロセスに用いられる上記の使い捨て装置のいずれにも当てはまる。
図1〜7は先行技術であり、図8〜17は本発明を示す。
1.光劣化が加速して寿命および/または測定精度/容量が制限されてしまうこと;
2、周囲光に対する感度;
3、運動または物理的摂動に対する感度
が含まれる。
i) 中空管状部およびベースプレート部を有するLDPAまたはEVAなどの高表面張力熱可塑性物質を含むポートを有し、ベースプレートは、自身の壁の孔においてバイオリアクタ容器と融合封止可能であり、センサアセンブリはさらに、
ii)少なくとも1つの電気および/または光学測定構成要素(光学部品のみが用いられる場合は全体的に不透明)を含むポリマーセンサ(監視)ユニットを有する。センサユニットは、ポートの中空管状部のボアの内部に収まり、以下に説明するような本発明に係る特別なプロセスを用いて、その内部に接着剤で保持される。好ましくは、監視ユニットには複数の測定構成要素が存在する。
・滅菌ガンマまたはベータ放射線にさらされた後の(光学特性および機械的特性における)安定性
・構造上の完全性
・低コスト
・USPクラスVI基準を満たす能力
・動物由来成分なし
・成形能力
・センサユニット本体の光学的な不透明度、および光伝送に必要な部分における光学的な透明度
を有する。
Claims (17)
- ポリマーバイオプロセス容器とともに用いる複合センサアセンブリであって、
i) 中空部およびベースプレート部を有する高表面張力熱可塑性物質を含むポートを備え、少なくとも前記ベースプレート部は、自身の壁の孔においてバイオプロセス容器と融合封止可能なポリマーを含み、前記センサアセンブリはさらに、
ii) 少なくとも1つの電気および/または化学および/または光学測定構成要素を含むポリマー監視センサユニットを備え、前記センサユニットは、ベース部を含み、全体的に皿型であり、前記ポートの中空部のボアの内部に収まるようにサイズ決めされ、その内部に接着剤で保持され、前記ポートは、監視センサユニット測定構成要素に、前記バイオリアクタ容器の中身へのアクセスを与え、当該構成要素は、入射する光信号および/または電気信号を提供する手段と、構成要素によって出射された測定信号を収集および送信する手段とを含み、
前記ポリマー監視センサユニットは、前記高表面張力熱可塑性物質とは異なる材料からなる、センサアセンブリ。 - 前記ポリマー監視センサユニットは、複数の電気および/または化学および/または光学測定構成要素を含む、請求項1に記載のセンサアセンブリ。
- 前記監視センサユニットは、自身のベースの上方に延在する周辺リムを有する、請求項1または2に記載のセンサアセンブリ。
- 前記センサユニットは、自身のベースの内面にカップ状の窪みを有し、および/または全体的に円形である、請求項1から3のいずれか1項に記載のセンサアセンブリ。
- 前記測定構成要素の各々は、前記センサユニットのベースの別個のカップ状の窪みに位置しており、前記カップ状の窪みの各々は、センサユニットベースの内面の上方に延在する周囲リムを含む、請求項2に記載のセンサアセンブリ。
- 前記カップ状の窪みの少なくとも1つは、自身の頂部の上に位置決めされた光シールドを有する、請求項5に記載のセンサアセンブリ。
- 前記センサユニットは、ポリカーボネート、コポリエステル、シクロオレフィンコポリマー、ポリオレフィン、およびポリスチレンからなるグループから選択されるポリマーを含む、請求項1から6のいずれか1項に記載のセンサアセンブリ。
- 前記ポートおよび前記センサユニットの界面の少なくとも一方は、互いに付着する前に、プラズマまたはUVオゾンまたは化学表面処理を受けている、請求項1から6のいずれか1項に記載のセンサアセンブリ。
- 前記界面同士の間にUSPクラスVI接着剤が存在する、請求項8に記載のセンサアセンブリ。
- 前記接着剤は、一液性または二液性のエポキシ樹脂、UV硬化エポキシ、シアノアクリレート、白銀硬化シリコーン、およびポリウレタンからなるグループから選択される、請求項9に記載のセンサアセンブリ。
- 前記高表面張力熱可塑性物質は、低密度もしくは超低密度ポリエチレン、またはエチレン酢酸ビニルコポリマーを含む、請求項1から10のいずれか1項に記載のセンサアセンブリ。
- 前記監視センサユニットは実質的に管状の構成であり、任意に、実質的に不透明のシースを含む、請求項1から11のいずれか1項に記載のセンサアセンブリ。
- 前記センサユニットは光シールドを有する、請求項1から12のいずれか1項に記載のセンサアセンブリ。
- 前記測定構成要素は、蛍光センサスポット、自由空間光学密度測定構成要素、光学分光測定構成要素、またはイオン感応性電界効果トランジスタ(ISFET)ユニットを含む、請求項1から13のいずれか1項に記載のセンサアセンブリ。
- ポリマーバイオプロセス容器とともに用いる複合アセンブリであって、
i) 中空部およびベースプレート部を有する高表面張力熱可塑性物質を含むポートを備え、少なくとも前記ベースプレート部は、自身の壁の孔においてバイオプロセス容器と融合封止可能なポリマーを含み、前記センサアセンブリはさらに、
ii) 回転アセンブリ構成要素および/またはスパージャー構成要素を含むポリマーユニットを備え、前記ユニットは、前記ポートの中空部のボアの内部に収まり、その内部に接着剤で保持されており、前記ポートは、ポリマーユニット構成要素に、前記バイオリアクタ容器の中身へのアクセスを与え、前記センサアセンブリはさらに任意に、
iii) バイオプロセス容器からサンプルを除去する手段を備える、複合アセンブリ。 - 前記ポリマーユニットは回転アセンブリ構成要素を含み、前記アセンブリは、バイオプロセス容器の内部の液体の攪拌のために用いられる、請求項15に記載の複合アセンブリ。
- 請求項1から16のいずれか1項に記載のセンサまたは複合アセンブリを製造するためのプロセスであって、ポートの管状部の内面およびアセンブリの外周面の少なくとも一方に、互いに付着させる前に、プラズマまたはUVオゾン洗浄を受けさせるステップと、任意に、一液性または二液性のエポキシ樹脂、UV硬化性エポキシ、シアノアクリレート、シリコーン、およびポリウレタンからなるグループから選択される接着剤を、前記内面および外面の一方または両方の間に介在させることによって付着させるステップとを備える、プロセス。
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2018523116A (ja) * | 2015-06-30 | 2018-08-16 | ローズマウント インコーポレイテッド | 使い捨て容器のための高分子遠隔シールシステム |
US10227555B2 (en) | 2006-08-02 | 2019-03-12 | Finesse Solutions, Inc. | Composite sensor assemblies for single use bioreactors |
US11827875B2 (en) | 2006-08-02 | 2023-11-28 | Finesse Solutions, Inc. | Method for manufacturing a composite sensor |
Families Citing this family (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9267100B2 (en) | 2006-08-02 | 2016-02-23 | Finesse Solutions, Inc. | Composite sensor assemblies for single use bioreactors |
US9261313B2 (en) * | 2013-03-20 | 2016-02-16 | Carleton Saunders | Method and system for controlling a heat transfer apparatus |
WO2015085214A1 (en) * | 2013-12-06 | 2015-06-11 | Pendo TECH | Sensor fitting for biotech process bag |
US10557811B2 (en) | 2013-12-06 | 2020-02-11 | Pendotech | Sensor fitting for biotech process bag |
US11181496B2 (en) | 2013-12-06 | 2021-11-23 | Pendotech | Sensor fitting for biotech process bag |
US9360422B2 (en) * | 2014-05-13 | 2016-06-07 | Asl Analytical, Inc. | Near-infrared optical probe and associated disposable sheath |
US9335000B2 (en) | 2014-05-30 | 2016-05-10 | Finesse Solutions, Inc. | Aseptic connectors for bio-processing containers |
US9346578B2 (en) | 2014-05-30 | 2016-05-24 | Finesse Solutions, Inc. | Aseptic connectors for bio-processing containers |
RU2670125C1 (ru) | 2015-04-13 | 2018-10-18 | Роузмаунт Аналитикал Инк. | Одноразовый биореакторный сосуд и способ изготовления одноразового биореакторного сосуда |
DE102015110893B3 (de) * | 2015-07-06 | 2016-03-24 | Sartorius Stedim Biotech Gmbh | Verfahren zum Messen einer Mehrzahl von Zustandsparametern eines in einem Behälter enthaltenen Fluids |
DE102015116617B4 (de) | 2015-09-30 | 2020-09-10 | Sartorius Stedim Biotech Gmbh | Sterile Verpackungseinheit und Verfahren zu deren Herstellung |
DE102015119756B3 (de) * | 2015-11-16 | 2016-11-17 | Sartorius Stedim Biotech Gmbh | Mischvorrichtung zum Durchmischen eines Bioreaktors aufweisend eine Synchronisierungseinrichtung |
DE102017010629A1 (de) | 2017-11-16 | 2019-05-16 | Sartorius Stedim Biotech Gmbh | Behälter mit Wandungsvorsprung und Sensorbereich |
DE102018117332A1 (de) * | 2018-07-18 | 2020-01-23 | Hamilton Bonaduz Ag | Vorrichtung zum Überwachen eines biologischen Prozesses in einem flüssigen Medium |
US11708555B2 (en) | 2019-05-30 | 2023-07-25 | Global Life Sciences Solutions Usa Llc | System, method and apparatus for draining a bioreactor vessel |
DE102019115147B4 (de) * | 2019-06-05 | 2021-01-14 | Schott Ag | Biokompatibles Verbundelement und Verfahren zur Herstellung eines biokompatiblen Verbundelements |
US20210016287A1 (en) * | 2019-07-16 | 2021-01-21 | Ge Healthcare Bio-Sciences Corp. | Reinforced bioreactor component structure system for cell cultivation |
US11499903B2 (en) | 2019-09-23 | 2022-11-15 | Nirrin Technologies, Inc. | In-situ probe |
JPWO2021131555A1 (ja) * | 2019-12-25 | 2021-07-01 | ||
DE102020107849A1 (de) | 2020-03-23 | 2021-09-23 | Zwilling J. A. Henckels Aktiengesellschaft | Messergriff mit Endfläche |
CN112974843B (zh) * | 2021-02-07 | 2021-10-22 | 江苏创英医疗器械有限公司 | 一种3d打印种植体的表面处理工艺 |
Family Cites Families (36)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4163903A (en) | 1977-10-27 | 1979-08-07 | Leeds & Northrup Company | Flame monitoring apparatus |
US4353488A (en) | 1980-04-21 | 1982-10-12 | Container Technologies, Inc. | Flexible container with displaceable fitting and probe coupler apparatus |
US4482585A (en) * | 1982-06-11 | 1984-11-13 | Toppan Printing Co., Ltd. | Container resistant to extremely low temperatures |
DE3905158A1 (de) | 1989-02-20 | 1990-08-30 | Wilkens Bremer Silberwaren Ag | Baukastensystem fuer bioreaktoren |
JP2907604B2 (ja) | 1991-10-07 | 1999-06-21 | 株式会社クボタ | 伝動ベルト |
US5798181A (en) * | 1994-10-04 | 1998-08-25 | W. L. Gore & Associates, Inc. | Fluoropolymer coated elastomeric rollers and structures |
JP3516400B2 (ja) | 1997-05-20 | 2004-04-05 | ザイムクエスト,インク. | 細胞処理システム |
US6190913B1 (en) | 1997-08-12 | 2001-02-20 | Vijay Singh | Method for culturing cells using wave-induced agitation |
US20020038925A1 (en) | 2000-04-14 | 2002-04-04 | Karl Reimer | Apparatus and method for continuous surface modification of substrates |
US6896778B2 (en) | 2001-06-04 | 2005-05-24 | Epocal Inc. | Electrode module |
US20040058453A1 (en) | 2002-09-20 | 2004-03-25 | 3M Innovative Properties Company | Reaction pouch comprising an analytical sensor |
GB0226863D0 (en) * | 2002-11-19 | 2002-12-24 | Biogene Ltd | Improvements in and relating to reaction vessels and reaction apparatus for use with such vessels |
DE10324956B4 (de) | 2003-06-03 | 2008-03-13 | Robert Bosch Gmbh | Messfühler |
WO2005068059A1 (en) | 2004-01-07 | 2005-07-28 | Levtech, Inc. | Mixing bag with integral sparger and sensor receiver |
EP1763575A4 (en) | 2004-04-27 | 2009-09-23 | Baxter Int | REACTOR SYSTEM WITH AGITATION RESERVOIR |
GB0410118D0 (en) | 2004-05-06 | 2004-06-09 | Glaxo Group Ltd | Novel bioreactor |
MXPA06014099A (es) | 2004-06-04 | 2007-05-09 | Xcellerex Inc | Sistemas biorreactores desechables y metodos. |
JP4437447B2 (ja) | 2005-02-22 | 2010-03-24 | 株式会社クボタ | トラクタのミッション構造 |
US20060240456A1 (en) | 2005-03-22 | 2006-10-26 | Liaohai Chen | Nanosized biological container and manufacture thereof |
US7879599B2 (en) | 2005-04-22 | 2011-02-01 | Hyclone Laboratories, Inc. | Tube ports and related container systems |
BRPI0520574A2 (pt) | 2005-09-30 | 2009-05-19 | Airbus Espana Sl | método de tratamento superficial de estruturas de material composto com feixes de plasma atmosférico |
DE102006001610B4 (de) | 2006-01-11 | 2009-06-10 | Sartorius Stedim Biotech Gmbh | Vorrichtung zur Befestigung eines Sensors an Behältern |
US7487688B2 (en) | 2006-03-20 | 2009-02-10 | Hyclone Laboratories, Inc. | Sampling ports and related container systems |
JP2010507068A (ja) * | 2006-04-21 | 2010-03-04 | バイエル・コーポレーシヨン | 原位置測定のための装置、システム、方法 |
US9267100B2 (en) | 2006-08-02 | 2016-02-23 | Finesse Solutions, Inc. | Composite sensor assemblies for single use bioreactors |
US8008065B2 (en) | 2006-08-02 | 2011-08-30 | Finesse Solutions, Llc. | Disposable bioreactor vessel port |
US10227555B2 (en) | 2006-08-02 | 2019-03-12 | Finesse Solutions, Inc. | Composite sensor assemblies for single use bioreactors |
US7824902B2 (en) * | 2006-11-03 | 2010-11-02 | Mark Selker | Optical interface for disposable bioreactors |
WO2008127429A2 (en) | 2006-11-21 | 2008-10-23 | Ge Healthcare Bio-Sciences Corp. | Assembling and utilizing rfid sensors in containers |
US7489402B2 (en) | 2006-12-04 | 2009-02-10 | Finesse Solutions, Llc | Optical collection geometries for phase fluorimetry |
GB0703175D0 (en) * | 2007-02-20 | 2007-03-28 | Ge Healthcare Bio Sciences Ab | Polymeric device suitable for ultraviolet detection |
US9109193B2 (en) | 2007-07-30 | 2015-08-18 | Ge Healthcare Bio-Sciences Corp. | Continuous perfusion bioreactor system |
FR2931838B1 (fr) | 2008-06-02 | 2010-06-11 | Millipore Corp | Installation pour traiter un liquide biologique. |
FR2934371B1 (fr) | 2008-07-25 | 2012-06-08 | Nanotec Solution | Dispositif capteur de biomasse a usage unique, procede de realisation de ce dispositif et bioreacteur a usage unique integrant ce capteur |
EP2513287B1 (en) | 2009-12-17 | 2016-10-12 | GE Healthcare Bio-Sciences AB | Sensor attachment arrangement for flexible bags |
CN101812405B (zh) | 2010-04-30 | 2013-05-22 | 惠倪 | 多边形塑料袋摇摆细胞反应器及培养动物细胞的方法 |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10227555B2 (en) | 2006-08-02 | 2019-03-12 | Finesse Solutions, Inc. | Composite sensor assemblies for single use bioreactors |
US11060055B2 (en) | 2006-08-02 | 2021-07-13 | Finesse Solutions, Inc. | Composite sensor assemblies for single use bioreactors |
US11827875B2 (en) | 2006-08-02 | 2023-11-28 | Finesse Solutions, Inc. | Method for manufacturing a composite sensor |
JP2018523116A (ja) * | 2015-06-30 | 2018-08-16 | ローズマウント インコーポレイテッド | 使い捨て容器のための高分子遠隔シールシステム |
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