JP5950277B2 - Cell and tissue transport equipment - Google Patents

Description

  The present invention relates to an apparatus for transporting cells and tissues collected from a living body and produced at a cell processing center.

  Currently, most regenerative medicine in Japan is based on autologous transplantation using the patient's own cells. In many cases, cells are collected from patients at medical institutions and used in universities. The cells are transported to a cell processing center (CPC) such as a company or a company, and the target cells and tissues are produced from the cells using the CPC. The produced cells and tissues are then transported back to the medical institution for transplantation into the patient.

  In such transport of cells and tissues, normal culture conditions such as temperature and pH are reproduced, physical impact on the cells during transport is reduced, and environmental deterioration in the cell holding container due to cell growth is prevented. Thus, in addition to reducing damage to cell activity, it is necessary to facilitate recovery of cells after transport.

  However, conventionally, none of the above-mentioned requirements are sufficiently satisfied.For example, anchorage-dependent cells are inoculated into a flask and transported while adhering to the culture surface. Vibration effects on cells during transport, such as direct transmission and damage to cell activity, have not been considered. In addition, cell growth during transportation is suppressed by lowering the control temperature, but cell activities other than growth cannot be maintained, and problems such as inability to perform gas control by controlling temperature only at low temperatures was there.

  There is a need for a device for transporting cells and tissues that solves such problems and accurately satisfies the above-described requirements.

Special table 2001-509002 gazette

  The present invention has been made in view of the above, and the object of the present invention is to reduce physical impact on cells and tissues during transportation and damage to cell activity, and facilitate recovery of cells after transportation. It is to provide a cell and tissue transport device that can be used.

To achieve the above object, the cell and tissue transport apparatus according to claim 1 is a cell and tissue transport apparatus used for transporting cells, wherein a high-density solution and a low-density solution in which an interface is formed. a cell retention container for accommodating the door, the cells held in said interface being arranged in the cell holding container spaced apart, possess a cell recovery colander for collecting the cells retained on the interface The high specific gravity solution is perfluorocarbon or modified silicone oil, and the low specific gravity solution is a medium for cell culture or a cell preservation solution .

The cell and tissue transport apparatus according to claim 1, wherein cells are formed at an interface formed between a high-density solution that is perfluorocarbon or modified silicone oil and a low-density solution that is a medium for cell culture or a cell preservation solution. In addition, the cell recovery tub is disposed away from the cells held at the interface, and the cells held at the interface are recovered by this cell recovery tub. Thus, the cells can be transported safely and without damage to the cell activity, and the cells held at the interface can be recovered easily and efficiently.
In addition, a high-density solution of perfluorocarbon or modified silicone oil and a low-density solution of physiological saline, which is a cell culture medium or cell storage solution, are placed in a cell holding container and placed between the high-density solution and the low-density solution. Since cells are suspended and held at the interface of the cells, physical impacts such as vibration to the cells during transportation and damage to cell activity can be reduced, and since no scaffold is provided, cell proliferation activity By stopping or lowering, it is possible to prevent environmental deterioration in the cell holding container due to cell proliferation.

  The cell and tissue transport device according to claim 2 is a container lid that closes the upper opening of the container so as to seal the cell holding container, and an air discharge unit disposed below the container lid. The air discharge hole formed in the center, conically spreads downward from the lower end of the air discharge hole, and the expanded lower end of the container collects the air in the cell holding container. An air discharge portion having a solution injection hole for introducing a solution into the cell holding container, and a conical expansion portion adjacent to the inner wall, and a peripheral portion of the conical expansion portion; An air discharge pipe that has a lower end inserted therethrough, an upper end projecting upward, and closely fitting into a cylindrical central recess formed in the center of the lower surface of the container lid, and an upper end penetrating upward through the solution injection hole. Protruding and near the periphery of the lower surface of the container lid Engaged closely fitted to made a cylindrical peripheral recess, the lower end extending downward, and summarized in that and a solution injection pipe for fixedly retaining the cells harvested bamboo basket.

  In the cell and tissue transport apparatus according to claim 2, an air discharge portion is disposed below the container lid for closing the cell holding container, and a conical shape is formed downward from a central air discharge hole of the air discharge portion. A conical spreading portion is provided, and while injecting the solution into the cell holding container from the solution injection pipe passing through the solution injection hole near the peripheral edge of the conical spreading portion, the air in the container is The collected air is discharged from the air discharge pipe that is passed through the air discharge hole, and the cell recovery basket is fixedly held at the lower end of the solution injection pipe that is passed through the solution injection hole. Can be discharged completely, and the shaking of the solution in the cell holding container due to vibration during transportation can be reduced, reducing the physical impact on the cells, and the cell recovery rod is fixed to the lower end of the solution injection pipe Kept Are therefore the cell recovery colander by lifting the air outlet with a solution injection pipe easily taken out, the cells can be easily recovered.

  The gist of the cell and tissue transport device according to claim 3 is that it has a heater formed on the peripheral wall portion of the cell holding container and for heating the temperature inside the cell holding container to a predetermined temperature.

  In the apparatus for transporting cells and tissues according to claim 3, a heater is formed on the peripheral wall portion of the cell holding container, and the temperature inside the cell holding container is heated to a predetermined temperature by this heater. The cells that are heated to a predetermined temperature and are held at the interface between the high specific gravity solution and the low specific gravity solution are also heated to a predetermined temperature, and the viability of the cells is kept high.

4. Symbol placement of cells and tissues of the transport device, said cell collection colander is, a gist that it is constituted by colander frame surrounding the outer periphery of the reticulated portion and reticulated portions of nylon mesh .

Transport device cells and tissues according to claim 4 Symbol mounting, since the mesh portion of said cell collection colander is composed of a nylon mesh, surrounded the outer periphery of the cell collection colander in colander frame, high density solution And the low specific gravity solution can be removed, and the cells can be efficiently recovered.

Billing transportation device 5. Symbol placement of cells and tissues is summarized in that applying a protein adsorption inhibitor to the inner wall of the bamboo basket frame.

Transport device cells and tissues according to claim 5 Symbol mounting, in order to apply the protein adsorption inhibitor to the inner wall of the colander frame surrounding the cell recovery colander, it is possible to prevent cell adhesion to cell recovery colander.

According to the present invention, the cells are suspended and held at the interface formed between the high specific gravity solution and the low specific gravity solution, and the cell recovery tub is disposed separately from the interface. Since the cells held in the cell are collected, physical impacts due to vibration during transportation are not transmitted to the cells, and the cells are not damaged, and the cells can be transported safely and floated at the interface. The retained cells can be collected easily and efficiently.
Further, according to the present invention, a high specific gravity solution such as perfluorocarbon or modified silicone oil and a low specific gravity solution such as a cell culture medium or a cell preservation solution (for example, physiological saline) are placed in a cell holding container. Since the cells are suspended and held at the interface between the specific gravity solution and the low specific gravity solution, physical impact such as vibration to the cells during transportation and damage to the cell activity can be reduced and the scaffolding can be reduced. Therefore, it is possible to prevent deterioration of the environment in the cell holding container due to cell growth by stopping or reducing the cell growth activity.

  Further, according to the present invention, the air discharge part is disposed below the container lid that closes the cell holding container, and the conical expanse spreads conically downward from the central air discharge hole of the air discharge part. While injecting the solution into the cell holding container from the solution injection pipe passed through the solution injection hole near the peripheral edge of this conical spreading part, air in the container is collected at the conical spreading part and discharged Air is discharged from the air discharge pipe that has passed through the hole, and the cell recovery tub is fixedly held at the lower end of the solution injection pipe that has passed through the solution injection hole, so that the air in the cell holding container is completely discharged. It can reduce the shaking of the solution in the cell holding container due to vibration during transportation, reduce the physical impact on the cells, and the cell recovery basket is fixedly held at the lower end of the solution injection pipe. Because the air discharge part Cells harvested colander easily removed by lifting with a solution injection pipe, the cells can be easily recovered.

  Furthermore, according to the present invention, the heater is formed on the peripheral wall portion of the cell holding container, and the temperature inside the cell holding container is heated to a predetermined temperature by this heater, so that the inside of the cell holding container is heated to the predetermined temperature. The cells that are warmed and held at the interface between the high specific gravity solution and the low specific gravity solution are also heated to a predetermined temperature, and the viability of the cells can be kept high.

  In addition, according to the present invention, since the mesh-like portion of the cell recovery basket is made of nylon mesh, and the outer peripheral edge of the cell recovery basket is surrounded by the hook frame, the high specific gravity solution and the low specific gravity solution are removed. The cells can be recovered efficiently.

  Furthermore, according to the present invention, since the protein adsorption inhibitor is applied to the inner wall of the cage frame surrounding the cell collection basket, cell adhesion to the cell collection basket can be prevented.

It is a perspective view which shows the external appearance of the transport apparatus of the cell and tissue concerning one Embodiment of this invention. It is a schematic diagram which shows the longitudinal cross-section which shows the internal structure of the transport apparatus of the cell and tissue shown in FIG. It is the perspective view and sectional drawing which show the structure of the inner lid used for the transport apparatus of the cell and tissue shown in FIG. It is a perspective view which shows the structure of the centrifuge tube and the inner cover cap which are used for the transport apparatus of the cell and tissue concerning other embodiment of this invention. It is a perspective view which shows the state which isolate | separated the centrifuge tube and inner cover cap shown in FIG. It is a longitudinal cross-sectional view which shows the screwing state of the centrifuge tube and inner cover cap shown in FIG. It is the perspective view and bottom view which show the structure of the air vent upper stage part provided at the upper part of the centrifuge tube shown in FIG. It is the front view and perspective view which show the cell collection tub used for the centrifuge tube shown in FIG. It is a perspective view which shows the state which isolate | separated only the centrifuge tube and inner cover cap shown in FIG. It is a schematic diagram which shows the longitudinal cross-section of the cell storage container used for the transport apparatus of the cell and tissue concerning another embodiment of this invention.

  DESCRIPTION OF EMBODIMENTS Hereinafter, embodiments for carrying out the present invention (hereinafter referred to as embodiments) will be described with reference to the drawings.

  FIG. 1 is a perspective view showing an appearance of a cell and tissue transport apparatus according to an embodiment of the present invention. The cell and tissue transport apparatus 1 shown in FIG. 1 is configured to be entirely surrounded by a cylindrical and large-diameter outer container 3 made of aluminum, stainless steel, or the like. The outer lid 31 fits closely, and the inside of the outer container 3 can be sealed.

  Further, as will be described later, the high specific gravity solution and the low specific gravity solution are accommodated in the outer container 3 so as to float and hold cells at the interface formed between the high specific gravity solution and the low specific gravity solution. A centrifuge tube 201, which is a cell holding container, is provided. The lower part of the centrifuge tube 201 is formed in a hemispherical shape or a conical shape, and a spring 33s is disposed below the centrifuge tube 201, and the centrifuge tube 201 is supported by the spring 33s with buffering properties.

  Further, a USB 35b is connected to the middle of the outer container 3 via an electric cable. This USB 35b is connected to a control unit (described later) inside the cell transport apparatus 1 via the electric cable. Connected to an external control device that supplies power and control signals to the cell transport device 1 via the USB 35b, and information from the cell transport device 1 is transmitted to the external control device via the USB 35b. It has become so. Further, an LED may be provided in the outer container 3 slightly above the portion to which the USB 35b is connected so that it can be determined whether or not the cell transport device 1 is in an activated state.

  FIG. 2 is a schematic view showing a longitudinal section showing the internal structure of the cell transport apparatus 1 shown in FIG. As shown in FIG. 2, the high specific gravity solution 9 and the low specific gravity solution 11 are placed in the centrifuge tube 201, and the two types of solutions placed in the centrifuge tube 201 sink downward. An interface 13 is formed between the high specific gravity solution 9 and the low specific gravity solution 11 floating above. The cell 15 is held on the interface 13 so as to float, and a scaffold is not provided for the cell 15, and physical impact due to vibration during transportation is not transmitted to the cell 15. The activity is not damaged.

  Further, below the interface 13, a cell recovery basket 17 is disposed in the high specific gravity solution 9 below the interface 13. Further, a lower end portion of a tube 21 extending vertically downward from the upper side is fixedly attached to the heel frame 19 surrounding the peripheral edge portion of the cell recovery tub 17.

  The cell collection basket 17 is made of, for example, a nylon mesh having a mesh size of 5 μm, and the cells 15 suspended and held on the interface 13 above the cell collection basket 17 are scooped up and efficiently collected as will be described later. It has come to be able to do. In addition, a protein absorption inhibitor is applied to the inside of the cocoon frame 19, thereby preventing cell adhesion to the cell collection tub 17.

  An inner lid 23 is fitted inside the upper end of the centrifuge tube 201. As shown in an enlarged view in FIGS. 3A and 3B, the inner lid 23 has an outer peripheral surface that is squeezed downward, and the squeezed lower end is located above the interface 13 in the low specific gravity solution 11. Opposite the interface 13.

  The inner lid 23 constitutes an air discharge portion, and as shown in FIGS. 3 (a) and 3 (b), it extends conically downward from the lower end portion of the air discharge hole formed in the center, The widened opening at the lower end has a conical portion 23b close to the inner wall of the centrifuge tube 201 so as to collect the air in the centrifuge tube 201, and is formed near the periphery of the conical portion 23b. A solution injection hole for injecting a solution is formed in 201, and the upper end of the tube 21 is inserted into the solution injection hole.

  A short air discharge pipe 23a is inserted into the air discharge hole in the center of the inner lid 23, and the upper end of the air discharge pipe 23a is sealed using a sealing sealer to form a sealing portion 23c. . In addition, the upper end of the tube 21 extending through the peripheral edge of the inner lid 23 is also sealed using a sealing sealer to form a sealing portion 21c.

  In addition, the inner lid 23 finishes discharging the air in the centrifuge tube 201 from the air discharge pipe 23 a while injecting the solution from the tube 21 into the centrifuge tube 201, and then from the air discharge pipe 23 a and the tube 21 to the inside of the centrifuge tube 201. As described above, the upper ends of the air discharge pipe 23a and the tube 21 are sealed by using a sealing sealer so as not to enter air. You may make it completely seal with a lid | cover. When the sealing sealer is used for sealing, the upper ends of the air discharge pipe 23a and the tube 21 are cut and opened with scissors or the like.

  As shown in FIG. 2, a film heater 33g, carbon 33f, an aluminum tape 33e, an aluminum plate 33d, and an acrylic cylinder 33c are provided on the outside of the centrifuge tube 201 from the inside so as to cover the entire outer periphery of the centrifuge tube 201. The layers are provided in the order of description. In addition, a heat insulating portion 33b made of foamed polystyrene, aluminum beads, or the like is provided between the acrylic cylinder 33c and the inner peripheral surface of the outer container 3 for heat insulation and buffer suppression. In addition, although the outer peripheral part of the centrifuge tube 201 and the film heater 33g are spaced apart from each other in the drawing, the centrifuge tube 201 is actually detachable and closely attached to some extent.

  Further, a temperature logger 31a is provided below the outer lid 31 that closes the upper opening of the outer container 3 and above the air discharge pipe 23a. The temperature logger 31a measures the temperature, It comes to record. A control unit 35 made of, for example, a one-chip microprocessor is provided on the inner peripheral surface of the outer container 3, and the USB 35b is connected to the control unit 35 via an electric cable. A thermostat 35a is attached to the outer peripheral portion of the acrylic cylinder 33c, and the thermostat 35a is connected to the control unit 35 via an electric cable.

  In the cell transport device 1 of the present embodiment configured as described above, the cells collected from the patient in the medical institution to be transported to the CPC are the high specific gravity solution 9 injected into the centrifuge tube 201 and the low specific gravity. It is held so as to float on the interface 13 formed between the solutions 11 as indicated by reference numeral 15.

  In addition, after injecting the high specific gravity solution 9 and the low specific gravity solution 11 into the centrifuge tube 201 as described above and holding the cells 15 suspended at the interface 13 between them, the air in the centrifuge tube 201 is completely discharged. Therefore, while the high specific gravity solution 9 or the low specific gravity solution 11 is additionally injected into the centrifuge tube 201 from the tube 21, the air in the centrifuge tube 201 is collected by the conical portion 23b, and the collected air is collected in the air discharge pipe 23a. The air in the centrifuge tube 201 is completely discharged. Then, the inner lid 23 is fitted into the upper opening of the centrifuge tube 201 in an airtight state, and the upper ends of the tube 21 and the air discharge pipe 23a are sealed with a sealing sealer.

  In this way, the cells 15 are suspended and held at the interface 13 between the high specific gravity solution 9 and the low specific gravity solution 11, and the air is evacuated from the centrifuge tube 201, and then the inside of the centrifuge tube 201 is airtight with the inner lid 23. After that, the centrifuge tube 201 is disposed in the outer container 3, and the lower end portion of the centrifuge tube 201 is placed on the spring 33s.

  Next, the film heater 33g is driven by the control unit 35 to generate heat, and this heat generation causes the high specific gravity solution 9 and the low specific gravity solution 11 in the centrifuge tube 201 to have a predetermined temperature, for example, a temperature of 25 ° C. to 37 ° C. The cells 15 held at the interface 13 between the high specific gravity solution 9 and the low specific gravity solution 11 are warmed to the predetermined temperature, and then the cells 15 are always kept at the predetermined temperature under the control of the control unit 35. The survival rate of the cells 15 is kept high.

  As described above, in the cell transport device 1 of the present embodiment, the cell transport device 1 is suspended while holding the cells 15 at the interface 13 in the centrifuge tube 201 and keeping the cells 15 at a predetermined temperature. For example, although it is transported to CPC by courier, etc., the cell transport device 1 of the present embodiment is extremely compact in which the centrifuge tube 201 containing the high specific gravity solution 9 and the low specific gravity solution 11 is accommodated in the outer container 3. The structure is simple and easy to handle in transportation. In this transportation, between the high specific gravity solution 9 and the low specific gravity solution 11 in the airtight centrifuge tube 201 in which air is completely discharged. Since the cells 15 are suspended and held at the interface 13, impacts such as vibrations on the cells during transportation and damage to the cell activity can be reduced, and a scaffold is not provided. By stopping or reducing the proliferation activity of the cells, at the same time as the prevention of environmental degradation cell retention in the vessel by cell growth is possible, it is possible to hold the cells dormant.

  Further, since the cells 15 are always maintained at a predetermined optimum temperature by the heating control under the control of the control unit 35, the cells 15 can be transported in a state in which the cell activity is maintained without causing a low-temperature injury as in the prior art. it can.

  Furthermore, after transporting the cell transport apparatus 1 to the CPC, it is necessary to recover the cells 15 from the cell transport apparatus 1 in order to produce tissue from the cells 15 in the CPC. When the outer lid 31 of the outer container 3 of the transport apparatus 1 is removed, the centrifuge tube 201 is taken out from the outer container 3, and the inner lid 23 is taken out of the removed centrifuge tube 201 so as to be fixed to the inner lid 23. At the same time, the tube 21 is lifted upward, and the cell recovery rod 17 fixed to the lower end of the tube 21 is simultaneously lifted upward while draining the high specific gravity solution 9 and the low specific gravity solution 11 from the mesh. Therefore, the cells 15 held at the interface 13 between the high specific gravity solution 9 and the low specific gravity solution 11 can be easily picked up by the cell recovery rod 17. It will be recovered.

  As described above, the cells 15 can be collected simply by lifting the inner lid 23 upward from the centrifuge tube 201 and can be performed very easily. Moreover, in the cell transport apparatus 1 of this embodiment, since PFC is used for the high specific gravity solution 9, gas control of the low specific gravity solution, that is, control by gas movement between the PFC and the culture medium or the preservation solution can be performed. It is possible to reduce damage to cell activity during transportation by using normal culture conditions such as temperature and pH.

  4 and 5 are perspective views showing a centrifuge tube used in a cell and tissue transport apparatus according to another embodiment of the present invention. FIG. 5 shows a lower centrifuge tube 201 and its upper part. The separated state is illustrated. The centrifuge tube 201 shown in FIGS. 4 and 5 contains a high specific gravity solution 9 and a low specific gravity solution 11 therein. Among these two types of solutions, the high specific gravity solution 9 that sinks downward and the low floating solution that floats above the high specific gravity solution 9. An interface 13 is formed between the specific gravity solution 11 and the specific gravity solution 11 as shown in FIG. 4, and the cells 15 are suspended and held on the interface 13 so that there is no scaffold for the cells 15. Thus, physical shocks due to vibration during transportation are not transmitted, and cell activity is not damaged.

  As shown in FIGS. 5 and 9, the centrifuge tube 201 has a threaded portion 201 a formed on the outer side of the upper end portion thereof. The threaded portion 201e is formed on the inner lid 213 that is tightly closed with the upper opening of the centrifuge tube 201 and an inner lid cap 123d that is fixedly attached and fixed with an adhesive or the like. Are screwed together. FIG. 6 illustrates this screwed state.

  As shown in FIG. 7, the inner lid 213 is formed such that a conical spreading portion 225 spreads conically from the lower end portion of a cylindrical portion 225 a formed at the center of the inner lid 213. This widened opening at the lower end collects air in the centrifuge tube 201.

  Further, an air vent pipe 123a is attached through the inner lid 213 and a central portion of the inner lid cap 123d fixed to the inner lid 213. An air vent cap 123c is tightly fitted to the upper end of the air vent pipe 123a to close the air vent pipe 123a tightly. The upper part of the air vent pipe 123a to which the inner lid cap 123d is fitted may be closed by a syringe 23c instead of the air vent cap 123c as shown in FIG.

  The lower end portion of the air vent pipe 123a is connected to the upper end portion of the cylindrical portion 225a above the conical spreading portion 225 in the inner lid 213. It can be passed.

  Near the peripheral edge of the inner lid cap 123d, an upper end of the tube 121 is provided so as to penetrate therethrough, and a tube cap 121c is attached to the upper end of the tube 121 so as to be tightly fitted. The lower part of the tube 121 penetrates the inner lid cap 123d, and further, the outside of a cell recovery rod 117 described later in the inner lid 213, that is, near the peripheral edge of the conical spreading portion 225 in the inner lid 213 is shown in FIG. The lower end of the tube hole 123f extends downward in the centrifuge tube 201. When the tube cap 121c is removed, external air taken in from the upper end of the tube 121 can be taken into the centrifuge tube 201 through the tube 121.

  As shown in FIG. 7, a cell collection basket 117 is disposed in the middle of the conical spreading part 225 of the inner lid 213, and the cell collection basket 117 is an inclined part of the conical spreading part 225. The upper corners are arranged so as to bite into. Further, as shown in FIG. 8, a cocoon frame 119 is provided at the peripheral portion of the cell collection tub 117. Note that the mesh portion of the cell recovery basket 117 is made of, for example, a nylon mesh having a mesh size of 5 μm, and the cells collected and held at the interface 13 between the high specific gravity solution 9 and the low specific gravity solution 11 by the cell recovery basket 117. 15 can be recovered. In addition, a protein absorption inhibitor is applied to the inside of the gutter frame 119 and the centrifuge tube 201, thereby preventing cell adhesion to the cell collection gutter 117.

  In the centrifuge tube 201 of the present embodiment configured as described above, the cells 15 are held while floating at the interface 13 between the high specific gravity solution 9 and the low specific gravity solution 11 in the centrifuge tube 201, and the inner lid cap After 123d is closely fitted so that the solution inside does not leak, the centrifuge tube 201 is accommodated in the cell transport device and transported to the CPC.

  In the CPC that has received the cell transport device containing the centrifuge tube 201, the cell 15 is collected from the centrifuge tube 201 in order to produce tissue from the cell 15. In this recovery, first, the air vent cap 123c and the tube cap 121c are removed, and a syringe (not shown) is attached to the upper end of the air vent pipe 123a instead of the air vent cap 123c.

  Then, the centrifuge tube 201 is turned upside down, and the high specific gravity solution 9 and the low specific gravity solution 11 are extracted with a syringe attached to the air vent pipe 123a. When the high specific gravity solution 9 and the low specific gravity solution 11 in the centrifuge tube 201 are extracted, the cells 15 suspended and held at the interface 13 of these solutions are recovered so as to ride on the cell recovery rod 117.

  After the cells 15 are collected in the cell collection basket 117 in this manner, a new centrifuge tube 201 is attached, the inverted state is restored to the original normal state, and the phosphate buffered saline (from the syringe attached to the air vent pipe 123a) ( PBS) is poured into the centrifuge tube 201, whereby the cells 15 collected in the cell collection basket 117 are poured into the centrifuge tube 201. The cells 15 are poured into a new centrifuge tube 201, and the centrifuge tube 201 is covered with a new lid, and then centrifuged to collect the cells 15.

  FIG. 10 is a schematic view showing a longitudinal section of a cell storage container used in a cell and tissue transport apparatus according to another embodiment of the present invention. The cell storage container 200 shown in the figure is configured such that the container body 222 can be closed in a sealed state by screwing the upper opening of the container body 222 and the screw formed inside the upper lid 223d. .

  The container body 222 is provided with a film heater 233f on the outside, and further provided with a heat insulating layer 233b on the outside thereof. With this configuration, the inside of the container body 222 can be heated to a predetermined temperature. Note that the film heater 233f is provided in close contact with the container.

  A cylindrical portion 311 is provided on the lower surface of the upper lid 223 d so that the upper end is close to the lower portion 223 d, and the lower portion of the cylindrical portion 311 is formed as an inner lid 223 of the container body 222. In addition, the inner lid 223 forms a conical spreading portion 223b that spreads conically downward from the lower end portion of the cylindrical portion 311. Is in close contact with the inner peripheral wall of the container body 222 so that the inner solution does not leak and is detachable.

  An air vent pipe 311 passes through and is attached to the center of the inner lid 223. An air vent cap 233c is tightly fitted to the upper end of the air vent pipe 311 to close the air vent pipe 311 tightly. The upper part of the air vent pipe 311 may be sealed and closed using a sealing sealer shown in FIG. 2 instead of the air vent cap 233c.

  The high specific gravity solution 9 and the low specific gravity solution 11 are placed inside the container main body 222 below the conical spreading portion 223b. Of the two types of solutions placed in the container body 222, an interface 13 is formed between the high specific gravity solution 9 that sinks below and the low specific gravity solution 11 that floats above the solution. The tissue piece 215 which is a cell sheet such as a skin tissue is suspended and held so that there is no scaffold for the tissue piece 215, and a physical impact due to vibration during transportation is transmitted to the tissue piece 215. In addition, the cellular activity is not damaged.

  In the cell storage container 200 of the present embodiment configured as described above, the container main body 222 is entirely closed with the inner lid 223, and the upper opening 223d is closed in a sealed state with the upper lid 223d. Since the high specific gravity solution 9 and the low specific gravity solution 11 are placed inside and the tissue piece 215 is suspended and held at the interface 13 between the high specific gravity solution 9 and the low specific gravity solution 11, The tissue piece 215 can be safely transported without transferring a physical impact due to vibration or the like to the tissue piece 215 during transportation.

  The embodiments of the present invention have been described with reference to the embodiments. However, the embodiments are exemplifications, and the scope of the invention described in the claims can be variously changed without departing from the gist of the invention. Is.

DESCRIPTION OF SYMBOLS 1 Cell transport apparatus 3 Outer container 9 High specific gravity solution 11 Low specific gravity solution 13 Interface 15 Cell 17 Cell recovery bowl 19 Persimmon frame 21 Tube 21c Sealing part 23 Inner lid 23a Air discharge pipe 23b Conical part 23c Sealing part 31 Outer lid 33b Heat insulation part 33c Acrylic cylinder 33d Aluminum plate 33e Aluminum tape 33f Carbon 33g Film heater 33s Spring 35 Control part 35b USB
117 Cell recovery rod 121 Tube 121c Tube cap 123a Air vent pipe 123c Air vent cap 123d Inner cap 200 Cell storage container 201 Centrifuge tube 213 Inner lid 215 Tissue piece 222 Container body 223 Inner lid 223b Conical expanse 225 Conical expanse Part 225a cylindrical part 233b heat insulation layer 233f film heater 311 cylindrical part

Claims (5)

A cell and tissue transport device used in transporting cells,
A cell holding container containing a high-density solution and a low-density solution in which an interface is formed;
Wherein the interface cells are retained disposed spaced apart the cell retention vessel, possess a cell recovery colander for collecting the cells retained on the interface,
The device for transporting cells and tissues, wherein the high specific gravity solution is perfluorocarbon or modified silicone oil, and the low specific gravity solution is a medium for cell culture or a cell preservation solution . A container lid for closing the upper opening of the container to seal the cell holding container;
An air discharge hole disposed in the lower part of the container lid and formed in the center, and extends downwardly from the lower end of the air discharge hole in a conical shape. An air discharge part having a solution injection hole for introducing a solution into the cell holding container, formed near the peripheral part of the conical extension part close to the inner wall and the conical extension part;
An air discharge pipe having a lower end inserted through the air discharge hole and an upper end protruding upward and closely fitting into a cylindrical central recess formed at the center of the lower surface of the container lid;
The upper end penetrates the solution injection hole and protrudes upward, closely fits into a cylindrical peripheral recess formed near the periphery of the lower surface of the container lid, and the lower end extends downward to hold the cell collection basket The cell and tissue transport device according to claim 1, further comprising a solution injection pipe.   3. The cell and tissue transport apparatus according to claim 1, further comprising a heater that is formed on a peripheral wall portion of the cell holding container and heats the temperature in the cell holding container to a predetermined temperature. . The cell collection colander is according to any one of claims 1乃optimum 3, characterized in that it is constituted by colander frame surrounding the outer periphery of the reticulated portion and reticulated portions of nylon mesh Cell and tissue transport equipment. The apparatus for transporting cells and tissues according to claim 4, wherein a protein adsorption inhibitor is applied to at least the inner wall of either the frame or the cell holding container.

Images