JP5901644B2 - 差次的な光吸収による生死判別細胞カウント方法 - Google Patents

差次的な光吸収による生死判別細胞カウント方法 Download PDF

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JP5901644B2
JP5901644B2 JP2013540047A JP2013540047A JP5901644B2 JP 5901644 B2 JP5901644 B2 JP 5901644B2 JP 2013540047 A JP2013540047 A JP 2013540047A JP 2013540047 A JP2013540047 A JP 2013540047A JP 5901644 B2 JP5901644 B2 JP 5901644B2
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light
image
cells
wavelength band
aliquot
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JP2014505465A (ja
JP2014505465A5 (https=
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シン ヘン
シン ヘン
ユージン ブレーニマン
ユージン ブレーニマン
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バイオ−ラッド ラボラトリーズ インコーポレーティッド
バイオ−ラッド ラボラトリーズ インコーポレーティッド
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1486Counting the particles

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
JP2013540047A 2010-11-18 2011-11-18 差次的な光吸収による生死判別細胞カウント方法 Expired - Fee Related JP5901644B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US41494710P 2010-11-18 2010-11-18
US61/414,947 2010-11-18
PCT/US2011/061363 WO2012068446A1 (en) 2010-11-18 2011-11-18 Viability cell counting by differential light absorption

Publications (3)

Publication Number Publication Date
JP2014505465A JP2014505465A (ja) 2014-03-06
JP2014505465A5 JP2014505465A5 (https=) 2014-09-18
JP5901644B2 true JP5901644B2 (ja) 2016-04-13

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JP2013540047A Expired - Fee Related JP5901644B2 (ja) 2010-11-18 2011-11-18 差次的な光吸収による生死判別細胞カウント方法

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Country Link
US (1) US8609363B2 (https=)
EP (1) EP2640843B1 (https=)
JP (1) JP5901644B2 (https=)
CA (1) CA2817887C (https=)
WO (1) WO2012068446A1 (https=)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8570370B2 (en) 2009-08-31 2013-10-29 Bio-Rad Laboratories, Inc. Compact automated cell counter
US8852524B2 (en) 2010-01-12 2014-10-07 Bio-Rad Laboratories, Inc. Cell counting slide with lateral reservoir for promoting uniform cell distribution
US9001200B2 (en) 2010-01-12 2015-04-07 Bio-Rad Laboratories, Inc. Cell characterization using multiple focus planes
US9354155B2 (en) 2011-05-31 2016-05-31 Bio-Rad Laboratories, Inc. Cell counting systems and methods
EP3256850B1 (en) 2015-02-09 2025-07-09 Slingshot Biosciences, Inc. Hydrogel particles with tunable optical properties and methods for using the same
WO2016196567A1 (en) * 2015-06-01 2016-12-08 Nexcelom Bioscience Llc Methods for cell count and viability measurements
EP3249406A1 (de) * 2016-05-27 2017-11-29 PerkinElmer Cellular Technologies Germany GmbH Verfahren zur bestimmung der anzahl von infektionsherden einer zellkultur
JP2020520647A (ja) * 2017-05-19 2020-07-16 スライブ バイオサイエンス, インコーポレイテッド 細胞を数えるためのシステムおよび方法
JP7759328B2 (ja) 2020-01-24 2025-10-23 スリングショット バイオサイエンシーズ, インコーポレイテッド 細胞様較正粒子のための組成物および方法
CA3177834A1 (en) 2020-05-04 2021-11-11 Jeffrey Kim Compositions and methods for passive optical barcoding for multiplexed assays
WO2023076036A1 (en) 2021-10-28 2023-05-04 Analiza, Inc. Partitioning systems and methods for determining multiple types of cancers
JP2024539956A (ja) 2021-10-29 2024-10-31 スリングショット バイオサイエンシーズ, インコーポレイテッド フィーダー細胞としておよび合成抗原提示細胞としてのヒドロゲル粒子
CN119907921A (zh) 2022-05-05 2025-04-29 弹弓生物科学公司 用于血液学的作为红细胞模拟物的工程化颗粒和含有工程化颗粒的组合物
AU2023280490A1 (en) 2022-06-02 2024-12-05 Slingshot Biosciences, Inc. Apoptotic cell mimic
EP4608872A2 (en) 2022-10-26 2025-09-03 Slingshot Biosciences, Inc. Size-tunable synthetic particles with tunable optical properties and methods for using the same for immune cell activation
WO2025049609A1 (en) 2023-08-29 2025-03-06 Slingshot Biosciences, Inc. Cd34 stem cell mimics

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7215451B1 (en) 1996-04-15 2007-05-08 Dai Nippon Printing Co., Ltd. Reflection type diffuse hologram, hologram for reflection hologram color filters, etc., and reflection type display device using such holograms
US6608682B2 (en) 1999-01-25 2003-08-19 Amnis Corporation Imaging and analyzing parameters of small moving objects such as cells
US6403378B1 (en) * 2001-04-26 2002-06-11 Guava Technologies, Inc. Cell viability assay reagent
KR100573621B1 (ko) 2003-07-18 2006-04-25 주식회사 디지탈바이오테크놀러지 세포 개체수 계수용 장치 및 그 제조방법
WO2005008226A1 (en) 2003-07-19 2005-01-27 Digital Bio Technology Device for counting micro particles
JP4728025B2 (ja) * 2005-03-29 2011-07-20 オリンパス株式会社 細胞画像解析装置
KR100766248B1 (ko) 2006-07-12 2007-10-12 동부일렉트로닉스 주식회사 이미지 소자 및 이의 제조 방법
US20080182290A1 (en) 2006-09-15 2008-07-31 Moshe Flugelman Apparatus and methods for determining viability of cell-based products
JP2008164551A (ja) * 2006-12-29 2008-07-17 Osaka Univ 細胞の評価方法、細胞測定用システム、及び細胞測定用プログラム

Also Published As

Publication number Publication date
WO2012068446A1 (en) 2012-05-24
JP2014505465A (ja) 2014-03-06
EP2640843A1 (en) 2013-09-25
EP2640843B1 (en) 2016-02-24
CA2817887A1 (en) 2012-05-24
EP2640843A4 (en) 2014-04-09
US20120295300A1 (en) 2012-11-22
CA2817887C (en) 2019-01-08
US8609363B2 (en) 2013-12-17

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