JP5836571B2 - Beer-taste beverage with a fruit-like aroma with herb-like and sweet apple-tea-like scents and method for producing the same - Google Patents

Description

  The present invention relates to a beer-taste beverage to which a fruit-like aroma with a herb-like and sweet apple-tea-like scent is imparted and a method for producing the same.

  It is known that the scent imparted to beverages has a great influence on the character formation of beverage products. For example, hops are known to impart a refreshing bitterness and aroma to beer, and the aroma derived from hops has a great influence on the character formation of beer-taste beverages such as beer and sparkling liquor. In recent years, attention has been paid to the unique “Fukumika” felt when drinking beer and the aroma after drinking, and research has been conducted on the use of hops and their effects on flavor.

  The hop aroma is composed of hop essential oil contained in hop lupulin grains. When a specific material example is given about hop essential oil, linalool is a typical terpene compound contained in hop essential oil, and is a substance which gives a floral feeling. In addition, about 300 or more kinds of compounds constituting essential oils such as myrcene and α-, β-pinene as monoterpenes, α-humulene and β-caryophyllene as sesquiterpenes have been identified to date.

  Thus, although existence of various aroma components derived from hops is known, studies on aroma and chemical components in beverages have been limited (see Non-Patent Documents 1 to 5). That is, the correspondence between specific fragrances and chemical components is not clear, and particularly chemical components related to fragmented fragrance characteristics are hardly known.

T. Kishimoto et al., J. Agric. Food Chem., 54, 8855-8861, 2006 G. T. Eyres et al., J. Agric. Food Chem., 55, 6252-6261, 2007 V. E. Peacock, et al., J. Agric. Food Chem., 28, 774-777, 1980 K. C. Lam et al., J. Agric. Food Chem, 34, 763-770, 1986 V. E. Peacock et al., J. Agric. Food Chem., 29, 1265-1269, 1981

  An object of the present invention is to provide a beer-taste beverage in which a fruit-like aroma with a herb-like and sweet apple-tea-like scent is felt, and a method for producing the same.

  The present inventors have controlled the content of a specific component in a beverage within a predetermined range, whereby a fruit-like fragrance, specifically, a fruit-like fragrance accompanied by a herb-like or sweet apple-tea-like fragrance. It was found that a beer-taste beverage can be produced.

  That is, according to the present invention, the following inventions are provided.

(1) (Z) -3-hexen-1-ol ((Z) -3-hexen-1-ol), linalool, geranyl acetate, β-citronellol, nerol Beer-taste beverage comprising at least nerol, heptanoic acid, β-ionone, isobutyl isobutyrate, and cis-linalool oxide Because
Concentration range of each component is (Z) -3-hexen-1-ol: 1.9 to 3.6 ppb
・ Linalool: 38.0-73.2ppb
Geranyl acetate: 3.0 to 5.8 ppb
Β-citronellol: 24.3-38.7 ppb
・ Nellore: 4.6-6.8ppb
-Heptanoic acid: 42.1-63.2 ppb
.Beta.-ionone: 0.13-0.14 ppb
Isobutyl isobutyrate: 37.6-62.1 ppb
Cis-linalool oxide: 4.5 to 9.4 ppb
And the concentration ratio of (Z) -3-hexen-1-ol, geranyl acetate, β-citronellol, nerol, heptanoic acid, β-ionone, isobutylisobutyrate, and cis-linalool oxide to linalool is (Z) -3-hexen-1-ol: 0.0493-0.051
Geranyl acetate: 0.0786 to 0.0791
Β-citronellol: 0.5284 to 0.6395
・ Nellore: 0.0925-0.121
Heptanoic acid: 0.8642 to 1.1062
Β-ionone: 0.0018 to 0.0037
Isobutyl isobutyrate: 0.849 to 0.9874
Cis-linalool oxide: 0.1189 to 0.1283
Beer-taste beverages (hereinafter referred to as “beverages according to the present invention”).

(2) The beer-taste beverage according to (1), which is a malt beverage.

(3) The beer-taste beverage according to (1) or (2), which is a fermented beverage.

(4) The method for producing a beer-taste beverage according to any one of (1) to (3), comprising fermenting a pre-fermentation solution to which at least hops have been added.

(5) Hops are: ・ Hexanoic acid, ethyl ester
・ Heptanoic acid, ethyl ester, and propanoic acid, 2-methyl-, propyl ester
And α-humulene,
・ 2-Tridecanone,
・ Butanoic acid, 2-methyl, 2-methylbutyl ester, and linalool
The production method according to (4), comprising at least one of the above.

  ADVANTAGE OF THE INVENTION According to this invention, the beer taste drink in which a fruit-like fragrance is felt, especially the beer taste drink in which the fruit-like fragrance accompanied by the herb-like and sweet apple tea-like fragrance is provided. As shown in the examples below, beer-taste beverages that have a fruit-like aroma with a herb-like, sweet apple-tea-like fragrance have not been known so far and are not found in commercial products. The present invention is advantageous in that it can provide a new type of beverage required by consumers. The beverage according to the present invention is also highlighted by a fruit-like aroma with a herb-like, sweet apple-tea-like aroma derived from hops, and has a new aroma in harmony with the sweet ester aroma of a beer-taste beverage Since it is also a beer-taste beverage, the present invention is advantageous in that it can provide a beer-taste beverage with a harmonious aroma.

It is the figure which showed the result of having analyzed the relationship between the test brewery product test sections E and F of Table 2, and the commercial products 1-18 of Table 5 by the principal component analysis.

Detailed description of the invention

Beer-taste beverages Beverages according to the present invention have nine flavor components, namely (Z) -3-hexen-1-ol, linalool, geranyl acetate, β-citronellol, nerol, heptanoic acid, β-ionone, isobutyliso It contains butyrate and cis-linalool oxide in a predetermined content. The content of these nine aroma components can be in the following ranges so that a fruit-like aroma with a herb-like, sweet apple tea-like aroma is clearly felt in the beverage according to the present invention. .

(Z) -3-hexen-1-ol: 1.9 to 3.6 ppb
・ Linalool: 38.0-73.2ppb
Geranyl acetate: 3.0 to 5.8 ppb
Β-citronellol: 24.3-38.7 ppb
・ Nellore: 4.6-6.8ppb
-Heptanoic acid: 42.1-63.2 ppb
.Beta.-ionone: 0.13-0.14 ppb
Isobutyl isobutyrate: 37.6-62.1 ppb
Cis-linalool oxide: 4.5 to 9.4 ppb

  The content of the aroma component in the beverage according to the present invention can be measured by gas chromatography with a mass spectrometer (GC / MS).

  In the beverage according to the present invention, the concentration ratio of the fragrance component to linalool can be set to the following range in order to make the fruit-like fragrance with a herb-like and sweet apple-tea-like fragrance more clearly felt. it can.

(Z) -3-hexen-1-ol: 0.0493-0.051
Geranyl acetate: 0.0786 to 0.0791
Β-citronellol: 0.5284 to 0.6395
・ Nellore: 0.0925-0.121
Heptanoic acid: 0.8642 to 1.1062
Β-ionone: 0.0018 to 0.0037
Isobutyl isobutyrate: 0.849 to 0.9874
Cis-linalool oxide: 0.1189 to 0.1283

  Furthermore, in order to make the fruit-like aroma with herb-like and sweet apple-tea-like aroma more clearly felt in the beverage according to the present invention, the content of the above nine kinds of aroma components is set within the above range. The concentration ratio of the aromatic component to linalool can be in the above range.

  In the present invention, “beer-taste beverage” refers to a beverage having a taste and aroma peculiar to beer obtained when beer is usually produced, that is, when beer is produced based on fermentation by yeast or the like. Examples include fermented malt beverages such as beer, happoshu, and liqueurs, and non-fermented malt beverages such as completely alcohol-free malt beverages.

  The beverage according to the present invention can be provided in the form of a fermented beverage. In the present invention, “fermented beverage” means a beverage fermented with yeast.

  The beverage according to the present invention can be provided in the form of an alcohol-containing beverage containing alcohol. In the present invention, the “alcohol-containing beverage” is used to include a fermented beverage obtained by fermentation with yeast and a beverage to which alcohol is added.

  The beverage according to the present invention can be provided in the form of a malt beverage. In the present invention, the “malt beverage” means a beverage mainly composed of wheat and / or wort obtained from malt, and includes a malt soft drink imparted with a refreshing feeling by carbon dioxide gas or the like. Examples of malt beverages include non-fermented malt beverages such as completely alcohol-free malt beverages having an alcohol content of 0% by weight, and alcohol-containing malt beverages containing alcohol. Examples of the alcohol-containing malt beverage include a fermented malt beverage obtained by fermentation and a malt beverage to which alcohol has been added. Examples of malt beverages include non-alcohol fermented malt beverages obtained by removing alcohol, other low-boiling components and low-molecular components from fermented malt beverages obtained by fermentation.

  In the present invention, the “fermented malt beverage” means a beverage fermented by yeast using a carbon source, a nitrogen source, water and the like as raw materials, and using at least malt as a raw material. Examples of such fermented malt beverages include beer, sparkling liquor, liqueur (for example, beverages classified as “liqueur (foaming) (1)” under the liquor tax law) and the like. The beverage according to the present invention can be preferably provided in the form of a fermented malt beverage using at least water, hops, and malt as raw materials. In addition, the drink obtained by mixing fermented drinks, such as fermented malt drink, and a non-fermented drink is also contained in the drink by this invention.

  The beverage according to the present invention is not particularly limited to a malt beverage as long as it is a beer-taste beverage, and can also be provided in the form of a non-malt beverage that does not use wheat or malt. In the present invention, the “non-wheat beverage” includes a soft drink imparted with a refreshing feeling by carbon dioxide gas or the like. Non-wheat beverages include non-fermented beverages such as completely alcohol-free beverages having an alcohol content of 0% by weight, and alcohol-containing beverages containing alcohol. Examples of the alcohol-containing non-wheat beverage include fermented beverages obtained by fermentation and beverages to which alcohol is added. Examples of non-wheat beverages include non-alcoholic fermented beverages obtained by removing alcohol, other low-boiling components and low-molecular components from fermented beverages obtained by fermentation.

  According to one preferred embodiment of the present invention, the beer-taste beverage is given a fruit-like aroma, and in a more preferred embodiment, a fruit-like aroma with a herb-like, sweet apple-tea-like aroma. Is given.

Manufacture of beverages Beverages according to the present invention comprise nine aroma components: (Z) -3-hexen-1-ol, linalool, geranyl acetate, β-citronellol, nerol, heptanoic acid, β-ionone, isobutyliso It can be produced by adding butyrate and cis-linalool oxide to a predetermined content. For example, a beverage obtained without undergoing a fermentation process with yeast can be made into a beverage according to the present invention by adding the above-mentioned nine aroma components.

  The acquisition source of these aromatic components is not particularly limited, and any commercially available product, one obtained by synthesis, or one isolated and purified from a natural product may be used.

  The beverage according to the present invention can also be produced by fermenting a pre-fermentation solution to which at least hops are added.

  When the beverage according to the present invention is a fermented malt beverage, it can be produced by fermenting a pre-fermentation solution comprising at least water, malt, and hops. In other words, fermented beer yeast is added to wort (pre-fermentation liquid) prepared from brewing raw materials such as malt and hops, followed by fermentation. By removing, the fermented malt beverage according to the present invention can be produced.

  In the above production procedure, wort can be produced according to a conventional method. For example, wort can be prepared by saccharifying a mixture of brewing raw materials and water, filtering to obtain wort, boiling the wort, and cooling the boiled wort. Hops can be added after boiling the wort or while boiling the wort.

  According to one preferable aspect of the present invention, in the production of the beverage of the present invention, hops can be added before boiling the pre-fermentation solution or after boiling the pre-fermentation solution.

As hops used in the production of the beverage according to the present invention,
Hexanoic acid, ethyl ester, heptanoic acid, ethyl ester, and propanoic acid, 2-methyl-, propyl ester, and α-humulene,
・ 2-Tridecanone,
-The thing which comprises at least 1 sort (s) of butanoic acid, 2-methyl, 2-methylbutyl ester, and linalool is mentioned. Such hops include, but are not limited to, phoenix-type hops (country of origin: United Kingdom).

  In order to make the fruit-like aroma with herb-like and sweet apple-tea-like aroma more clearly felt in the beverage according to the present invention, the hop addition conditions can be set as follows, for example.

・ Add about 6 g / L of hops and boil the wort for about 5 minutes.

-After boiling the wort, add 2 g / L or more of hops when standing in a general whirlpool. Preferably, after boiling the wort, assuming a general whirlpool standing, the wort is allowed to stand at 80 to 95 ° C. for 20 to 60 minutes.

  After boiling, by adding hops so as to be 5 g / L or less at the time of standing, the strength of the hop aroma did not become excessive, and a tendency to improve the degree of preference, that is, the preference was seen.

  In the method for producing a fermented malt beverage according to the present invention, in addition to hops and malt, auxiliary materials defined by liquor tax law such as rice, corn, corn, potato, starch, saccharides (for example, liquid sugar), protein degradation products and yeast Nitrogen sources such as extracts, fragrances, pigments, foaming / foaming improvers, water quality modifiers, fermentation aids and other additives can be used as brewing materials. Further, ungerminated barley (for example, ungerminated barley (including an extracted one), ungerminated wheat (including an extracted one)) may be used as a brewing raw material. The obtained fermented malt beverage should be (i) distilled under reduced pressure or atmospheric pressure to remove alcohol and low boiling components, or (ii) remove alcohol and low molecular components with a reverse osmosis (RO) membrane. Can also be used as a non-alcohol fermented malt beverage.

  When the beverage according to the present invention is a beer-taste fermented beverage that does not use wheat or malt, it is produced by fermenting a pre-fermentation solution comprising at least water and hops according to the production procedure of the fermented malt beverage. Can do. In addition to water and hops, carbon sources (for example, sugars such as liquid sugar) and nitrogen sources (for example, amino acid sources such as protein degradation products and yeast extract) can be added to the pre-fermentation solution. Accordingly, other additives such as fragrances, pigments, foaming / foaming improvers, water quality modifiers, fermentation aids, and the like can be added. The obtained beer-taste fermented beverage is (i) distilled under reduced pressure or atmospheric pressure to remove alcohol and low-boiling components, or (ii) alcohol and low-molecular components are removed with a reverse osmosis (RO) membrane. By this, it can also be set as a non-alcohol beer taste fermented drink.

  According to another aspect of the present invention, nine aroma components are included: (Z) -3-hexen-1-ol ((Z) -3-hexen-1-ol), linalool, geranyl acetate (Geranyl acetate), β-citronellol, nerol, heptanoic acid, β-ionone, isobutyl isobutyrate, and cis-linalool oxide There is provided a method for adjusting a beverage to a fruit-like aroma with a herb-like and sweet apple-tea-like scent by adjusting (cis-linalool oxide) to a predetermined content in the beverage. Adjustment of content in the drink of an aromatic component may add these aromatic components, and may adjust it by selecting the kind of hop used as a raw material.

Moreover, the drink of this invention can also be manufactured as follows, for example.
Test brewing of fermented beverages using hops and analyzing aroma components in advance. From the result, a multiple regression equation with the fragrance component when the addition timing and addition amount of hops are used as variables is obtained. It can be produced by fermenting the pre-fermentation solution produced at the hop addition amount and the addition time, which are calculated using the multiple regression equation and each fragrance component falls within the desired range. At this time, the hop is
・ Hexanoic acid ethyl ester,
Comprising heptanoic acid ethyl ester, and propanoic acid, 2-methyl-, propyl ester,
・ Α-humulene,
・ 2-Tridecanone,
It is preferable to use one comprising at least one of 2-methyl butanoate, 2-methylbutyl ester, and linalool. More preferably, the hop is used alone.

  The present invention will be specifically described based on the following examples, but the present invention is not limited to these examples.

Test Example 1: Aroma confirmation test and exploration of aroma components An aroma test after beer brewing was conducted on 60 varieties of hops. The aroma of hops of Phoenix (Phoenix) (country of origin: UK) was herb-like, sweet apple It has been found that it is a fruit-like aroma accompanied by a tea-like aroma (hereinafter, sometimes referred to as “the aroma targeted by the present invention”).

  Subsequently, the fragrance component was further searched for 60 varieties of hops subjected to the fragrance test, and fragrance components having different concentrations between the hop varieties were searched. In the search, gas chromatography with a mass spectrometer (GC / MS) was used. Specifically, the fragrance component was extracted from the test sample with a C18 solid phase column and subjected to GC / MS. The internal standard method was used for quantification. Borneol was used as an internal standard substance and added to 25 ppb in the sample. The analysis conditions of the hop aroma component in GC / MS were as follows.

[GC / MS analysis conditions]
Capillary column: Trade name: HP-INNOWAX (length 60 m, inner diameter 0.25 mm, film thickness 0.25 μm)
Oven temperature: 40 ° C., 0.3 min-3 ° C./min→240° C., 20 min Carrier gas: He, 10 psi low pressure air transfer line temperature: 240 ° C.
MS ion source temperature: 230 ° C
MSQ pole temperature: 150 ° C
Front inlet temperature: 200 ° C
Monitoring ion: ion used for the same quantification as quantitative ion
Borneol m / z = 110
(Z) -3-hexen-1-ol m / z = 82
linalool m / z = 93
Geranyl acetate m / z = 93
β-Citronellol m / z = 69
Nerol m / z = 93
Heptanoic acid m / z = 60
β-Ionone m / z = 177
Isobutyl isobutyrate m / z = 89
Cis-Linalool Oxide m / z = 94

As a result of the search, it was found that several tens of components correspond to components having different concentrations between varieties. Some of them were as described in Table 1 below.

  Multivariate statistical analysis, such as cluster analysis, was performed using the multivariate analysis software “STATISTICA” for fragrances characterized by a fruit-like aroma with herb-like and sweet apple-tea-like scents. In addition to confirming the correlation between each component (data omitted), confirm the aroma characteristics of each aroma component, and finally the following 9 types of aroma components may be suitable as the target aroma index of the present invention Was judged to be high.

Fruit-like aroma: (Z) -3-hexen-1-ol ((Z) -3-hexen-1-ol), β-citronellol, β-nerol, isobutyl isobutyrate ), Geranyl acetate, cis-linalool oxide
・ Sweet fruit-like aroma: β-ionone
・ Floral fragrance: linalool
・ Herbal-like aroma: heptanoic acid

Example 1: Brewing Example (a) Brewing Method The storage sample used for evaluation was prepared using a 1.5 L scale apparatus. Charged wort adjusted to 13.5 degrees was used for the brewing test (67% malt use ratio, 33% use of secondary ingredients (rice, corn grits, corn starch)). In the brewing test, the prepared wort is boiled, and a predetermined amount of phoenix seed hop (source: Barth) is added during boiling or at the time of standing after boiling (test sections A, B, C, D, E in Table 2 below). F). Specifically, the prepared wort was heated and boiled with an electric heater while adjusting the boiling strength to be constant and the evaporation rate to be 10% in 60 minutes. After boiling, the same amount of water as the amount of evaporation was added to the sample, and the wort was allowed to stand at 95 ° C. for 60 minutes. After filtering the filter paper, brewer's yeast was added to the wort that had been cooled with ice water, and the sample subjected to main fermentation for 1 week and post-fermentation for 4 days was used as a tasting sake sample.

  In test sections E and F, hops were added after boiling, and then allowed to stand at 95 ° C. for 1 hour.

(B) Component analysis and sensory evaluation The analysis of the aroma component of the obtained sake sample was performed by gas chromatography with a mass spectrometer (GC / MS) according to Test Example 1.

  Sensory evaluation of the obtained sake sample was performed by tasting with three skilled sensory evaluation panels. In sensory evaluation, the characteristic of fragrance is expressed as the intensity of “fruit-like fragrance with herb-like, sweet apple-tea-like fragrance”, 5: feel strong and clear, 3: feel, 1: feel weak, 0 : I did not feel, scored.

The addition time and amount of hops, aroma component analysis values, and sensory evaluation results were as shown in Table 2 below.

  Regarding the sensory evaluation results, as shown in Table 2, the strongest 5 was obtained in the test section E, and 4 was obtained in the test section F. In Test Zones C and D, 3 was obtained, and in Test Zone A, 2 was obtained. It was 1 in Test Zone B.

  In addition, the drinks in the test areas E and F are newly harmonized with the scent of sweet esters of beer-taste beverages, with emphasis on fruit-like scents with herb-like and sweet apple-tea-like scents derived from hops. It was confirmed that it is a beer beverage having an aroma.

(C) Multiple regression analysis Multiple regression analysis (multiple regression analysis) based on the concentration of each aroma component and hop addition conditions, that is, boiling time (minutes) after addition (hereinafter simply referred to as “boiling time”) and addition amount (g / L) Variable analysis software “STATISTICA”: Statsoft). In addition, since the test sections E and F were added after boiling, the “boiling time” was set to “0.1” and statistical analysis was performed. The results were as shown in Table 3.

Therefore, the multiple regression equation shown in the table is represented by the following equation for each component.
Predicted value of component = << coefficient (hour) x (boiling time (minute)) >> + << coefficient (amount) x (added amount (g / L)) >> + intercept Specific examples are as follows.
(Z) -3-Hexen-1-ol = <<-0.0319 × (boiling time (min)) >> + << 0.4200 × (addition amount (g / L)) >> + 1.2291
Linalool = << -1.8419 x (boiling time (min)) >> + << 4.5833 x (added amount (g / L)) >> + 36.4766

  The multiple correlation coefficient showed a high value of 0.91 or more for many components. Based on the coefficient of the multiple regression equation, the relation of each component with the aroma and the effectiveness as an index were examined. Here, if the target component is a volatile aroma component, the longer the boiling time of the hop, the smaller the component value, so the coefficient (time) is negative, the component value increases as the addition amount increases As the number increases, the coefficient (amount) should be positive.

  According to Table 3, the components for obtaining the target fragrance of the present invention are (Z) -3-hexen-1-ol, linalool, geranyl acetate, β-citronellol, nerol, heptanoic acid, β-ionone, It was judged to be composed of isobutyl isobutyrate and cis-linalool oxide.

  Further, from the results of Tables 2 and 3, it is considered that the desirable ranges of the respective aroma component values are as follows.

(Z) -3-hexen-1-ol: 1.9 to 3.6 ppb
・ Linalool: 38.0-73.2ppb
Geranyl acetate: 3.0 to 5.8 ppb
Β-citronellol: 24.3-38.7 ppb
・ Nellore: 4.6-6.8ppb
-Heptanoic acid: 42.1-63.2 ppb
.Beta.-ionone: 0.13-0.14 ppb
Isobutyl isobutyrate: 37.6-62.1 ppb
Cis-linalool oxide: 4.5 to 9.4 ppb

(D) Control of aroma component using multiple regression equation Using the regression equation obtained in the multiple regression analysis performed in (c) above, what conditions are set so that each aroma component value falls within a desirable range A simulation was performed to see if As a result, it was found that in the case of addition at a boiling time of 5 minutes, a desired range was obtained with hop addition of 5.4 to 6.1 g / L. More specifically, in the case of addition at a boiling time of 5 minutes, (Z) -3-hexen-1-ol is 3.6 ppb, linalool is 54.8 ppb, and geranyl acetate is 6 g / L hop addition. 5.6 ppb, β-citronellol 32.7 ppb, nerol 5.7 ppb, β-ionone 0.13 ppb, isobutylisobutyrate 45.7 ppb, cis-linalool oxide 5.6 ppb, and heptanoic acid 61 0.0 ppb, indicating that each fragrance component is in the desired range. Further, in the case of addition at the time of standing after boiling, that is, addition in a whirlpool, (Z) -3-hexen-1-ol is 1.9 ppb, and linalool is 44. even by adding 1.7 g / L of hops. 1 ppb, geranyl acetate 3.0 ppb, β-citronellol 26.4 ppb, nerol 5.0 ppb, β-ionone 0.13 ppb, isobutyl isobutyrate 41.4 ppb, cis-linalool oxide 5.0 ppb, and It was found that heptanoic acid was 43.4 ppb, and each fragrance component was in a desirable range.

(E) Composition ratio of fragrance component The composition ratio of each component was obtained from Table 2 based on linalool that is commonly present in hops and related to floral or hoppy fragrance. The results were as shown in Table 4.

  From Table 4, the range of the composition ratio of each fragrance component based on linalool is considered as follows.

(Z) -3-hexen-1-ol: 0.0493-0.051
Geranyl acetate: 0.0786 to 0.0791
Β-citronellol: 0.5284 to 0.6395
・ Nellore: 0.0925-0.121
Heptanoic acid: 0.8642 to 1.1062
Β-ionone: 0.0018 to 0.0037
Isobutyl isobutyrate: 0.849 to 0.9874
Cis-linalool oxide: 0.1189 to 0.1283

Test Example 2: Comparison with commercial products The aromatic components of 18 types of commercial products (beer, happoshu, miscellaneous sake, liqueur) were analyzed. Specifically, analysis of the aroma component of a commercial product was performed by gas chromatography with a mass spectrometer (GC / MS) according to Test Example 2. The results were as shown in Table 5. In addition, n. d. Indicates that it was not detected.

  Although the concentration of the fragrance component of the commercial product is within the range of the desired fragrance component concentration specified in Example 1 (c), that is, within the range of the beer-taste beverage according to the present invention, the value is shown in bold and underlined, There was no commercial product with all fragrance components within the range at the same time. That is, when all the fragrance components are within the range of the beer-taste beverage according to the present invention, it is a beer-taste beverage having the fragrance targeted by the present invention, but any commercially available product has at least one fragrance component according to the present invention. Was outside the concentration range of beer-taste beverages. In addition, as a result of sensory evaluation, there was no product that clearly felt a fruit-like aroma with herb-like and sweet apple-tea-like scents (data not shown). Therefore, it was confirmed from the component analysis and sensory evaluation test that there is no commercially available product having the fragrance targeted by the present invention.

Next, the composition ratio based on linalool was calculated based on the analysis values in Table 5. The results were as shown in Table 6.

  A value in which the composition ratio of the fragrance component of the commercial product is within the range of the desirable composition ratio specified in Example 1 (e), that is, within the range of the composition ratio of each fragrance component of the beer-taste beverage according to the present invention is bold. As shown by the underline, there was no commercial product in which all fragrance components were within the range at the same time. Therefore, it was confirmed from the composition ratio of the component analysis values that there is no commercially available product having the fragrance targeted by the present invention.

Furthermore, in order to chart the relationship between the test brewery test sections E and F in Table 2 and the commercial products 1 to 18 in Table 5, each component value was analyzed by principal component analysis (multivariate analysis software “STATISTICA”: Stat Software). Analysis). Table 7 shows the eigenvectors, eigenvalues, contribution rates, and cumulative contribution rates of the principal component analysis results. The contribution ratio was 69% for factor 1, 13% for factor 2, and the cumulative contribution ratio for factors 1 and 2 reached 83%. FIG. 1 shows a scatter diagram of factor 1 and factor 2. Test plots E and F, which had good aromas, were clearly distinguishable from commercial products. This indicates that none of the commercially available products have the same component characteristics. As a result of the sensory test, the commercially available products do not have the target fragrance. I was able to verify.

Test Example 3: Analysis of hop component The component analysis of the phoenix seed hop used in Example 1 was performed. Specifically, the essential oil component of Phoenix seed hop was fractionated and extracted with aqueous solutions having different ethanol concentrations, test brewing was performed, and the fraction from which the aroma targeted by the present invention was obtained was identified. The components contained in the fraction were analyzed in the GC / MS scan mode according to the procedure of Test Example 1. As a result, it was found that the fraction that causes the aroma targeted by the present invention among the components contained in the Phoenix seed hop is composed of the components shown in Table 8.
Table 8: Components contained in the essential oil fraction for obtaining the target fragrance of the present invention: 2-pentanone, 3-methyl-
・ 3-buten-2-ol, 2-methyl-
・ Propanoic acid, 2-methyl-, propyl ester
・ Butanoic acid, 3-methyl-, ethyl ester
Propanic acid, 2-methyl-, 2-methylpropyl ester (propanoic acid, 2-methyl-, 2-methylpropyl ester)
・ 1,6-octadiene, 2,7-dimethyl-
・ 1-butanol, 2-methyl-, acetate
・ Β-myrcene
・ 1-butanol, 3-methyl-, propanoate
・ Propanoic acid, 2-methyl-, pentyl ester
・ Propanoic acid, 2-methyl-, 2-methylbutyl ester
・ Hexanoic acid, ethyl ester
・ Butanoic acid, 2-methyl-, 2-methylbutyl ester
・ Pentyl 3-methylbutanoate
・ Cyclohexanone
2-buten-1-ol, 3-methyl- (2-buten-1-ol, 3-methyl-)
・ Pinol
・ Heptanoic acid, ethyl ester
・ Methyl 4-methylhexa-2-enoate
・ 6-Methyl-5-hepten-2-one
・ 1-hexanol
・ 3-pentanone, 2,4-dimethyl-
・ 2-nonanone
・ Tetradecane
・ Benzene, 1,3-bis (1,1-dimethylethyl)-(benzene, 1,3-bis (1,1-dimethylethyl)-)
・ Linalool oxide
・ 1-Heptanol
・ 2-butoxyethyl acetate
・ Trans-linlool oxide
・ Α-copaene
・ Linalool L
・ Propanoic acid, 2-methyl-
・ Hexadecane
・ 2-undecanone
・ Caryophyllene
・ Benzoic acid, methyl ester
・ 1-hexadecene
・ Α-humulene
・ Β-selinene
・ Α-selinene
・ Δ-cadinene
・ Octadecane
・ 2-tridecanone
・ Eicosane

  Next, the component that causes the fragrance targeted by the present invention contained in the Phoenix hops is replaced with other varieties that differ from the fragrance targeted by the present invention, such as Willamette, Super Pride, and Pride of When compared with components such as Ringwood species, Nelson Sobin species, Galaxy species, Hercules species, etc., it was found that the characteristic component groups that are abundantly included in the Phoenix species are as follows.

(1) Ingredients unique to Phoenix species (unusual for other varieties)
・ Hexanoic acid, ethyl ester
・ Heptanoic acid, ethyl ester
(2) Ingredients common to a small number of other varieties: propanoic acid, 2-methyl-, propyl ester
(3) Common components derived from hops, also included in other varieties, α-humulene
・ 2-tridecanone
・ Butanoic acid, 2-methyl-, 2-methylbutyl ester
・ Linaroll

  From the above, in order to obtain the target fragrance of the present invention, all the components of (1) are necessary for the hop component, and one of the common components of (2) and one of the common components of (3) The above was considered necessary.

Claims (6)

(Z) -3-hexen-1-ol ((Z) -3-hexen-1-ol), linalool, geranyl acetate, β-citronellol, nerol A beer-taste beverage comprising at least heptanoic acid, β-ionone, isobutyl isobutyrate, and cis-linalool oxide ,
The concentration range of each component is
(Z) -3-hexen-1-ol: 1.9 to 3.6 ppb
・ Linalool: 38.0-73.2ppb
Geranyl acetate: 3.0 to 5.8 ppb
Β-citronellol: 24.3-38.7 ppb
・ Nellore: 4.6-6.8ppb
-Heptanoic acid: 42.1-63.2 ppb
.Beta.-ionone: 0.13-0.14 ppb
Isobutyl isobutyrate: 37.6-62.1 ppb
Cis-linalool oxide: 4.5 to 9.4 ppb
And the concentration ratio of (Z) -3-hexen-1-ol, geranyl acetate, β-citronellol, nerol, heptanoic acid, β-ionone, isobutylisobutyrate, and cis-linalool oxide to linalool is (Z) -3-hexen-1-ol: 0.0493-0.051
Geranyl acetate: 0.0786 to 0.0791
Β-citronellol: 0.5284 to 0.6395
・ Nellore: 0.0925-0.121
Heptanoic acid: 0.8642 to 1.1062
Β-ionone: 0.0018 to 0.0037
Isobutyl isobutyrate: 0.849 to 0.9874
Cis-linalool oxide: 0.1189 to 0.1283
A range of beer-taste beverages,
The beer-taste beverage is produced by a production method comprising fermenting a pre-fermentation solution to which at least hops are added, and the hop is hexanoic acid, ethyl ester,
・ Heptanoic acid, ethyl ester, and propanoic acid, 2-methyl-, propyl ester
Comprising
・ Α-humulene,
・ 2-tridecanone,
・ Butanoic acid 2-methyl, 2-methylbutyl ester, and linalool
A beer-taste beverage comprising a phoenix hop . Is a malt beverage, beer-taste beverage according to claim 1. The beer taste drink of Claim 1 or 2 which is a fermented drink. Fermenting a pre-fermentation solution to which at least hops are added,
The hop is hexanoic acid, ethyl ester,
・ Heptanoic acid, ethyl ester, and propanoic acid, 2-methyl-, propyl ester
Comprising
・ Α-humulene,
・ 2-tridecanone,
・ Butanoic acid 2-methyl, 2-methylbutyl ester, and linalool
A beer-taste beverage, which is a phoenix hop ,
The beer-taste beverage comprises (Z) -3-hexen-1-ol ((Z) -3-hexen-1-ol), linalool, geranyl acetate, β-citronellol ), Nerol, heptanoic acid, β-ionone, isobutyl isobutyrate, and cis-linalool oxide,
The concentration range of each component is
(Z) -3-hexen-1-ol: 1.9 to 3.6 ppb
・ Linalool: 38.0-73.2ppb
Geranyl acetate: 3.0 to 5.8 ppb
Β-citronellol: 24.3-38.7 ppb
・ Nellore: 4.6-6.8ppb
-Heptanoic acid: 42.1-63.2 ppb
.Beta.-ionone: 0.13-0.14 ppb
Isobutyl isobutyrate: 37.6-62.1 ppb
Cis-linalool oxide: 4.5 to 9.4 ppb
And the concentration ratio of (Z) -3-hexen-1-ol, geranyl acetate, β-citronellol, nerol, heptanoic acid, β-ionone, isobutylisobutyrate, and cis-linalool oxide to linalool is (Z) -3-hexen-1-ol: 0.0493-0.051
Geranyl acetate: 0.0786 to 0.0791
Β-citronellol: 0.5284 to 0.6395
・ Nellore: 0.0925-0.121
Heptanoic acid: 0.8642 to 1.1062
Β-ionone: 0.0018 to 0.0037
Isobutyl isobutyrate: 0.849 to 0.9874
Cis-linalool oxide: 0.1189 to 0.1283
The manufacturing method of the beer taste drink which is the range of. The manufacturing method of Claim 4 whose beer taste drink is a malt drink. The manufacturing method of Claim 4 or 5 whose beer taste drink is a fermented drink.