JP5744177B2 - 画像中の物体をセグメンテーションする方法 - Google Patents
画像中の物体をセグメンテーションする方法 Download PDFInfo
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- JP5744177B2 JP5744177B2 JP2013503713A JP2013503713A JP5744177B2 JP 5744177 B2 JP5744177 B2 JP 5744177B2 JP 2013503713 A JP2013503713 A JP 2013503713A JP 2013503713 A JP2013503713 A JP 2013503713A JP 5744177 B2 JP5744177 B2 JP 5744177B2
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T7/00—Image analysis
- G06T7/10—Segmentation; Edge detection
- G06T7/11—Region-based segmentation
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T7/00—Image analysis
- G06T7/10—Segmentation; Edge detection
- G06T7/143—Segmentation; Edge detection involving probabilistic approaches, e.g. Markov random field [MRF] modelling
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T7/00—Image analysis
- G06T7/10—Segmentation; Edge detection
- G06T7/155—Segmentation; Edge detection involving morphological operators
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
- G06V10/00—Arrangements for image or video recognition or understanding
- G06V10/20—Image preprocessing
- G06V10/26—Segmentation of patterns in the image field; Cutting or merging of image elements to establish the pattern region, e.g. clustering-based techniques; Detection of occlusion
- G06V10/267—Segmentation of patterns in the image field; Cutting or merging of image elements to establish the pattern region, e.g. clustering-based techniques; Detection of occlusion by performing operations on regions, e.g. growing, shrinking or watersheds
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
- G06V20/00—Scenes; Scene-specific elements
- G06V20/60—Type of objects
- G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
- G06V20/695—Preprocessing, e.g. image segmentation
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T2207/00—Indexing scheme for image analysis or image enhancement
- G06T2207/10—Image acquisition modality
- G06T2207/10056—Microscopic image
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T2207/00—Indexing scheme for image analysis or image enhancement
- G06T2207/10—Image acquisition modality
- G06T2207/10064—Fluorescence image
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T2207/00—Indexing scheme for image analysis or image enhancement
- G06T2207/20—Special algorithmic details
- G06T2207/20036—Morphological image processing
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T2207/00—Indexing scheme for image analysis or image enhancement
- G06T2207/20—Special algorithmic details
- G06T2207/20076—Probabilistic image processing
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T2207/00—Indexing scheme for image analysis or image enhancement
- G06T2207/20—Special algorithmic details
- G06T2207/20112—Image segmentation details
- G06T2207/20152—Watershed segmentation
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T2207/00—Indexing scheme for image analysis or image enhancement
- G06T2207/30—Subject of image; Context of image processing
- G06T2207/30004—Biomedical image processing
- G06T2207/30024—Cell structures in vitro; Tissue sections in vitro
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- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Theoretical Computer Science (AREA)
- General Physics & Mathematics (AREA)
- Computer Vision & Pattern Recognition (AREA)
- Multimedia (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Probability & Statistics with Applications (AREA)
- Software Systems (AREA)
- Image Analysis (AREA)
- Image Processing (AREA)
Description
以下は、本方法及びシステムの様々な実施形態を示すために使われる、限定的ではない実施例である。
本方法は、異種移植モデルに適用された場合、図5A〜図5Dに示すように同様の結果をもたらす。図5A〜図5Dは、異種移植モデルに対応するDAPI画像を示す。図5A及び図5Bは、それぞれ、元の画像及びセグメンテーション画像である。図6C及び図6Dは、それぞれ、元の画像及びセグメンテーションである。示されている形状、大きさ及び外観の違いは、蛍光色素の不均一性のためである。本方法からのセグメンテーション結果は、細胞核が集まり、重なり、頻繁に接している画像においてでさえ、極めて正確で一貫性がある。図6に示すように、細胞50、36、37は、明確に区別可能である。
Claims (11)
- デジタル画像を複数の対象物体にセグメンテーションする方法であって、
前記画像の複数の確率分布図であって各々異なるセグメンテーション分類子から得られた複数の確率分布図を生成するステップと、
前記複数の確率分布図に基づいて合成確率分布図を生成するステップと、
1以上の局所物体最大値に基づいて複数の像点をマッピングするステップと、
前記マッピングした点に少なくとも部分的に基づく1以上の物体制約を適用して局所物体情報を同定するステップと、
前記局所物体情報及び背景マスクが得られたら、前記合成確率分布図に1以上の領域閾値を適用して、画像を複数の領域にセグメンテーションするステップと、
少なくとも部分的に、セグメンテーションされた領域を対応する局所物体最大値と結合することによってセグメンテーション画像を生成するステップと、
前記セグメンテーション画像をデジタルデバイスに少なくとも一時的に格納又は表示するステップと
含む方法。 - 前記物体制約の少なくとも1つが形態学的制約である、請求項1記載の方法。
- 前記形態学的制約が細胞核に基づく、請求項2記載の方法。
- 前記対象物体が生物学的細胞である、請求項1乃至請求項3のいずれか1項記載の方法。
- セグメンテーション分類子が、大きさ、形状、強度、テクスチャ、ウェーブレット及びフラクタルからなる群から選択される、請求項1乃至請求項4のいずれか1項記載の方法。
- 前記合成確率分布図が、前記複数の確率分布図の加重平均に基づく、請求項1乃至請求項5のいずれか1項記載の方法。
- 前記加重平均が経験的に定義される、請求項6記載の方法。
- 前記加重平均が予め定義されている、請求項6記載の方法。
- 前記セグメンテーション分類子の少なくとも1つが物体曲率に基づく、請求項1乃至請求項8のいずれか1項記載の方法。
- 前記セグメンテーション分類子の少なくとも1つが、ガウス関数及び調和関数から少なくとも部分的に得られる1組のデジタルフィルタである、請求項9記載の方法。
- 前記セグメンテーション分類子の1つ以上が、勾配、強度、ウェーブレット又はフラクタルに基づく、請求項9記載の方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US12/757,668 | 2010-04-09 | ||
US12/757,668 US8300938B2 (en) | 2010-04-09 | 2010-04-09 | Methods for segmenting objects in images |
PCT/SE2011/050407 WO2011126442A2 (en) | 2010-04-09 | 2011-04-06 | Methods for segmenting objects in images |
Publications (3)
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JP2013524361A JP2013524361A (ja) | 2013-06-17 |
JP2013524361A5 JP2013524361A5 (ja) | 2015-05-07 |
JP5744177B2 true JP5744177B2 (ja) | 2015-07-01 |
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JP2013503713A Expired - Fee Related JP5744177B2 (ja) | 2010-04-09 | 2011-04-06 | 画像中の物体をセグメンテーションする方法 |
Country Status (5)
Country | Link |
---|---|
US (1) | US8300938B2 (ja) |
EP (1) | EP2556491B1 (ja) |
JP (1) | JP5744177B2 (ja) |
CN (1) | CN102834846A (ja) |
WO (1) | WO2011126442A2 (ja) |
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EP2556491A2 (en) | 2013-02-13 |
EP2556491B1 (en) | 2020-07-29 |
US20110249883A1 (en) | 2011-10-13 |
WO2011126442A3 (en) | 2011-11-24 |
US8300938B2 (en) | 2012-10-30 |
WO2011126442A2 (en) | 2011-10-13 |
JP2013524361A (ja) | 2013-06-17 |
EP2556491A4 (en) | 2017-07-05 |
CN102834846A (zh) | 2012-12-19 |
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