JP5727081B1 - Cosmetics and skin cosmetics - Google Patents

Abstract

[PROBLEMS] To provide a cosmetic and a skin cosmetic that can easily improve the feel of skin and hair. Cosmetics and skin cosmetics contain a heat-denatured product of fish-derived collagen. Cosmetics and skin cosmetics contain a tuna-derived collagen heat-denatured product and a tie-derived collagen heat-denatured product. [Selection figure] None

Description

  The present invention relates to a cosmetic and a skin cosmetic.

  Conventionally, cosmetics containing fish-derived collagen are known (see, for example, Patent Documents 1 and 2). Patent Document 1 discloses collagen derived from wrinkles, wrinkles, or wrinkles. Patent Document 2 discloses yellowfin-derived collagen. Patent Documents 3 to 5 disclose Thai-derived collagen.

Japanese Patent Application Laid-Open No. 09-278639 JP 2000-256398 A JP 2003-327599 A JP 2004-59437 A Japanese Patent Laid-Open No. 2003-212897

By the way, cosmetics containing collagen are widely known, but there is still room for improvement in terms of enhancing the effects of cosmetics based on the inclusion of collagen.
This invention is made | formed in view of such a situation, The objective is to provide the cosmetics and skin cosmetics which are easy to improve the touch of skin or hair.

A cosmetic that solves the above problems is a cosmetic that contains a heat-denatured product of fish-derived collagen, and the tuna-derived collagen is heat-denatured under a temperature range of 45 to 55 ° C. for 0.5 to 2 hours. A heat-denatured product of tuna-derived collagen and a heat-denatured product of Thai-derived collagen obtained by heat-denaturing Thai-derived collagen under conditions of a temperature range of 45 to 55 ° C. and 0.5 to 2 hours .
A skin cosmetic that solves the above problems is a skin cosmetic containing a heat-denatured fish-derived collagen, and the tuna-derived collagen is subjected to a temperature range of 45 to 55 ° C. under conditions of 0.5 to 2 hours. It contains a heat-denatured tuna-derived collagen that has been heat-denatured, and a heat-denatured product of tie-derived collagen that has been heat-denatured from 45 to 55 ° C. in the temperature range of 0.5 to 2 hours .

  According to the present invention, it is easy to improve the feel of skin and hair.

Hereinafter, embodiments of cosmetics and skin cosmetics will be described.
The cosmetic contains a heat-denatured product of fish-derived collagen. Fish-derived collagen includes a heat-denatured tuna-derived collagen and a heat-denatured Thai-derived collagen.

  The heat-denatured tuna-derived collagen is obtained by heat denaturation treatment in which tuna-derived collagen is heated. The tuna-derived collagen is a water-soluble collagen obtained from fish skin (dermis) or scales of tuna (Perciformidae). Examples of tuna include yellowfin, albacore, bigeye, bluefin tuna, Atlantic bluefin tuna, cocinaga, and southern bluefin tuna.

  A heat-denatured product of Thai-derived collagen is obtained by a heat-denaturing treatment in which Thai-derived collagen is heated. Thailand-derived collagen is a water-soluble collagen obtained from fish skin (dermis) or scales of Thailand (Perciformes, Periformidae). Examples of the tie include red sea bream, red sea bream, Japanese red sea bream, red sea bream, red sea bream, yellow millet, black sea bream, red sea bream, and red sea bream.

  The heat-denatured tuna-derived collagen and the heat-denatured Thai-derived collagen have a structure in which most or all of the triple helical structure of collagen has been dissolved. Thermally denatured tuna-derived collagen and Thai-derived collagen contain a polypeptide chain (α chain), an α chain dimer (β chain) and an α chain trimer (γ chain). However, it contains little or no hydrolyzate having a lower molecular weight than the polypeptide chain.

The denaturation rate in the heat-denatured tuna-derived collagen and the heat-denatured Thai-derived collagen is, for example, about 85 to 100%. The modification rate is measured by a circular dichroism dispersometer.
The heat-denatured tuna-derived collagen is preferably a product obtained by heat-denaturing tuna-derived collagen under conditions of a temperature range of 45 to 55 ° C. and 0.5 to 2 hours.

The heat-denatured product of Thai-derived collagen is preferably a product obtained by heat-denaturing Thai-derived collagen under conditions of a temperature range of 45 to 55 ° C. and 0.5 to 2 hours.
The heat denaturation treatment is performed by heating the aqueous collagen solution. This aqueous solution may contain, for example, a pH adjuster, a thickener and the like.

  The heat denaturation treatment of tuna-derived collagen and the heat denaturation treatment of tie-derived collagen may be performed separately or simultaneously using an aqueous solution containing tuna-derived collagen and tie-derived collagen.

  The mass ratio of tuna-derived collagen heat-denatured to Thai-derived collagen heat-denatured in cosmetics was 100 parts by mass of the total amount of tuna-derived collagen heat-denatured and Thai-derived collagen heat-denatured. It is expressed in mass parts of heat-denatured collagen derived from tie. The mass part of the heat-denatured tuna-derived collagen is preferably in the range of 10 to 90 parts by mass, more preferably 10 to 70 parts by mass, and even more preferably 20 to 50 parts by mass. When the mass part of the heat-denatured tuna-derived collagen is 10 parts by mass or more and 90 parts by mass or less, the effect based on the combined use of the two kinds of heat-denatured substances is more likely to increase.

  The total content of tuna-derived collagen heat-denatured and Thai-derived collagen heat-denatured in the cosmetic is preferably 0.002% by mass or more. The total content of tuna-derived collagen heat-denatured and Thai-derived collagen heat-denatured in the cosmetic is preferably 20% by mass or less, more preferably 18% by mass or less, and still more preferably. It is 15 mass% or less.

  The cosmetic is configured as a skin cosmetic or a hair cosmetic. Skin cosmetics include scalp cosmetics. Examples of skin cosmetics include lotion, milky lotion, beauty essence, cream, face wash, pack, lotion, face wash, foundation, lipstick, lip balm, eye cream, body lotion, hair tonic, scalp treatment, hair restorer. And bath agents. Examples of hair cosmetics include shampoo, rinse, hair treatment, hair foam, hair gel, hair water, hair wax, hair cream, hair color, hair bleach, and permanent wave agent.

The dosage form of the cosmetic is not particularly limited, and examples of the dosage form include liquid (solution, emulsion, etc.), cream, gel, foam, semi-solid, and solid.
The cosmetic is prepared by mixing a cosmetic base with a heat-denatured tuna-derived collagen and a heat-denatured Thai-derived collagen.

  Examples of cosmetic bases used in skin creams that are examples of cosmetics include hydrocarbons, oils and fats, esters, fatty acids, phospholipids, higher alcohols, nonionic surfactants, glycols, and purified water. It is done.

  Examples of the hydrocarbon include squalane, liquid paraffin, and petrolatum. Examples of the fat include macadamia nut oil, shea fat, olive oil, coconut oil, evening primrose oil, jojoba oil, castor oil, and hardened castor oil. Examples of the ester include isopropyl myristate, isopropyl palmitate, cetyl octanoate, glyceryl trioctanoate, octyldodecyl myristate, octyl stearate, stearyl stearate, and glyceryl stearate. Examples of fatty acids include lauric acid, myristic acid, palmitic acid, stearic acid, and behenic acid. Examples of phospholipids include lecithin and hydrogenated lecithin. Examples of the higher alcohol include myristyl alcohol, cetanol, cetostearyl alcohol, stearyl alcohol, and behenyl alcohol.

  Examples of the nonionic surfactant include polyoxyalkylene alkyl ether, polyoxyalkylene alkylphenyl ether, glycerin fatty acid ester, and polyoxyalkylene hydrogenated castor oil. Examples of glycols include glycerin, diglycerin, 1,3-butylene glycol, ethylene glycol, and polyethylene glycol.

  For cosmetics, in addition to the above, for example, cationic surfactants, anionic surfactants, amphoteric surfactants, polymer compounds, lower alcohols, plant extracts, sequestering agents, preservatives, viscosity modifiers , PH adjusters, colorants, ultraviolet absorbers, low molecular weight compounds, fats and oils, amino acids, solvents, fragrances and the like can be contained as necessary.

  The cosmetics of the present embodiment are provided as cosmetics stipulated in the Pharmaceutical Affairs Law or quasi drugs (for example, medicated cosmetics). That is, the cosmetic can contain components that are permitted by the Pharmaceutical Affairs Law.

  The cosmetic of this embodiment is applied to skin and hair according to a conventional method. At this time, since the cosmetic contains a heat-denatured product of tuna-derived collagen and a heat-denatured product of collagen derived from Thailand, either a heat-denatured product of tuna-derived collagen or a heat-denatured product of Thai-derived collagen It becomes easier to improve the feel of skin and hair than a cosmetic containing one. Moreover, the combined use of the heat-denatured tuna-derived collagen and the heat-denatured Thai-derived collagen is a moisturizing component compared to the case of using either the tuna-derived collagen heat-denatured or the Thai-derived collagen heat-denatured. As advantageous.

The operational effects exhibited by the above-described embodiment will be described below.
(1) Since the cosmetic of the present embodiment contains a tuna-derived collagen heat-denatured product and a tie-derived collagen heat-denatured product, it is easy to improve the feel of skin and hair. According to the cosmetic of the present embodiment, for example, the sticky feeling of skin and hair is easily suppressed and a moist feeling is easily obtained.

  (2) Since the cosmetic of the present embodiment contains a tuna-derived collagen heat-denatured product and a tie-derived collagen heat-denatured product, the skin moisturizing effect is easily obtained. Skin cosmetics are also expected to have an effect of making wrinkles less noticeable and an increase in skin elasticity (harness) based on a moisturizing effect.

  (3) A composition containing a heat-denatured product of tuna-derived collagen and a heat-denatured product of Thai-derived collagen can be used by mixing with a cosmetic base, thereby providing a moisturizing cosmetic. For this reason, the cosmetic raw material containing the heat-denatured tuna-derived collagen and the heat-denatured Thai-derived collagen is advantageous as a moisturizer used by mixing with a cosmetic base.

  (4) When a heat-denatured product of tuna-derived collagen and a heat-denatured product of collagen derived from Thailand are used in combination, compared to the case of using either a heat-denatured product of tuna-derived collagen or a heat-denatured product of Thai-derived collagen, The result that the expression level of COL1A1 which is an expression gene of type I collagen is increased is obtained. For this reason, the combined use of the heat-denatured tuna-derived collagen and the heat-denatured Thai-derived collagen is expected to be advantageous from the viewpoint of, for example, enhancing the self-healing ability of the skin. It is expected that the skin quality will be improved based on the self-healing ability of the skin. Therefore, for example, the skin cosmetic of the present embodiment is expected to have an effect of improving wrinkles, tarmi, firmness, or rough skin.

(Example of change)
The above embodiment may be modified as follows.
The cosmetic is prepared by mixing a cosmetic base with a heat-denatured tuna-derived collagen and a heat-denatured Thai-derived collagen. The cosmetic may be prepared by, for example, heat-denaturing tuna-derived collagen and tie-derived collagen by heating a mixture of the cosmetic base, tuna-derived collagen and tie-derived collagen.

  -At least one of the tuna-derived collagen and the Thai-derived collagen may be subjected to atelo-treatment. The atelo-treatment is a process for removing telopeptides possessed by collagen by enzyme treatment.

-At least one of tuna-derived collagen and Thai-derived collagen may be subjected to chemical decoration such as succinylation.
-The cosmetic may further contain a heat-denatured product of fish-derived collagen other than a heat-denatured product of tuna-derived collagen and a heat-denatured product derived from Thailand.

  -The cosmetic may be composed of a plurality of compositions. For example, the cosmetic comprises a first composition containing a tuna-derived collagen heat-denatured product and a Thai-derived collagen heat-denatured product, and a second composition containing a cosmetic base. May be changed. In the case of this cosmetic, at the time of use, the first composition and the second composition are mixed and then applied to the body.

Next, the technical idea that can be grasped from the above embodiment will be described below.
(I) A moisturizing agent used in combination with a cosmetic base, which contains a heat-denatured product of fish-derived collagen, which is derived from Thailand and tuna-derived collagen. A moisturizing agent containing a heat-denatured product of collagen.

(B) A moisturizing cosmetic containing a heat-denatured product of fish-derived collagen, which contains a heat-denatured tuna-derived collagen and a heat-denatured product of Thai-derived collagen.
(C) A skin quality improving cosmetic containing a heat-denatured product of collagen derived from fish, comprising a heat-denatured product of tuna-derived collagen and a heat-denatured product of Thai-derived collagen, wrinkles, tarmi, Or a skin quality improving cosmetic that improves rough skin.

Next, examples and comparative examples will be described.
<Effect of heat-denatured products of fish-derived collagen on collagen expression genes>
Each fish-derived collagen shown in Table 1 was heat-denatured at 50 ° C. for 1 hour. About the obtained heat-denatured product, the influence with respect to the expression level of COL1A1 which is an expression gene of a type I collagen was confirmed using the normal human skin fibroblast (NHDF) monolayer culture system. The expression level of COL1A1 was expressed by correcting the expression level of ACTB (β-actin) as an internal standard. The results are shown in the column “COL1A1 expression level” in Table 1.

From the results shown in Table 1, it is understood that the heat denatured product of yellowfin-derived collagen and the heat denatured product of Thai-derived collagen have a higher expression level of COL1A1 than the heat-denatured products of other fish-derived collagen.

  Next, for the above gene expression level, a comparison test of a heat-denatured collagen-derived collagen and a heat-denatured Thai-derived collagen, a heat-denatured collagen-derived collagen, and a heat-denatured collagen-derived collagen alone Went. The heat-denatured product used in this comparison test was heat-denatured at 50 ° C. for 1 hour. About the obtained heat-denatured product, the influence with respect to the expression level of COL1A1 which is an expression gene of a type I collagen was confirmed using the co-culture system of three-dimensional cultured skin and normal human skin fibroblasts (NHDF). The expression level of COL1A1 after 48 hours was expressed by correcting with the expression level of ACTB (β-actin) as an internal standard. The results are shown in the column “COL1A1 expression level” in Table 2.

From the results shown in Table 2, it is expected that the effect of improving the skin quality is enhanced by the combined use of the heat-denatured collagen-derived collagen and the heat-denatured collagen derived from Thailand.

  Here, regarding the heat-denatured collagen, the relationship between the heat denaturation treatment conditions and the molecular weight was confirmed using SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Using heather-derived collagen as an example, each heat-denatured product was prepared by heat-denaturing under conditions of 50 ° C. for 0.5 hour, 50 ° C. for 1 hour, and 50 ° C. for 2 hours. From the result of SDS-PAGE of each heat-denatured product, it corresponds to the polypeptide chain (α chain), α chain dimer (β chain), and α chain trimer (γ chain) found in collagen molecules. Three bands were confirmed, but almost no increase was observed over time in the hydrolyzate having a lower molecular weight than that of the polypeptide chain.

(Example 1 and Comparative Examples 1 and 2)
In Example 1 and Comparative Examples 1 and 2, skin creams as cosmetics were prepared.
The cosmetic base was prepared using the following components 1-14. First, after heating the oil phase component of the following components 1-10 and the water phase component of the following components 11-14 to 75-80 degreeC, respectively, the oil phase component was mixed with the water phase component of the stirring state. Next, the obtained mixture was cooled while stirring with a homogenizer to prepare a cosmetic base as an emulsion.

Component 1: Squalane Component 2: Macadamia nut oil Component 3: Shea fat Component 4: Glyceryl stearate Component 5: Stearic acid Component 6: Hydrogenated lecithin Component 7: Behenyl alcohol Component 8: POE (20) octyl ether Component 9: Stearic acid ( 10) Glycerin Component 10: PEG (60) hydrogenated castor oil Component 11: Butylene glycol Component 12: Glycerin Component 13: Preservative Component 14: Purified water Tuna-derived collagen (collagen-derived collagen, trade name: Sea Gem Collagen, Katakura Chikkarin The tuna-derived collagen was heat-denatured by heating a mixture of a viscosity modifier and a mixture of 50% at 50 ° C. for 1 hour. Thereby, a collagen heat-denatured product solution A was prepared.

  Thermal denaturation of Thai-derived collagen by heating a mixture of Thai-derived collagen (red sea bream-derived collagen, trade name: Marinegen S-06, manufactured by Nitta Gelatin Co., Ltd.) and a viscosity modifier at 50 ° C. for 1 hour. Processed. Thereby, a collagen heat-denatured product solution B was prepared.

  The cosmetics of each example were prepared by mixing the cosmetic base with at least one of the collagen heat-denatured product solution A and the collagen heat-denatured solution B. The total collagen heat-denatured content in the cosmetic of each example is 0.0225% by mass. Table 3 shows the types and mass ratios of the heat-denatured collagen in the cosmetics of each example.

  The mass part of the heat-denatured tuna-derived collagen (A) shown in the table below indicates the mass part of a solution containing 0.3% by mass of the heat-denatured tuna-derived collagen. Moreover, (B) the mass part of the heat | fever denaturation material of a tie origin collagen shows the mass part of the solution containing 0.6 mass% of the heat | fever denaturation material of a tie origin collagen.

<Measurement of water content>
The cosmetic of Example 1 was applied to the inner part of the forearm of five panelists (men and women aged 23 to 42) under the conditions of an application area of 4 × 4 cm and an application amount of 30 μL. Each cosmetic of Comparative Examples 1 and 2 was also applied to five panelists in the same manner as in Example 1. About the paneler's skin, the moisture content before and behind apply | coating cosmetics was measured using the skin surface angle layer moisture content measuring apparatus (Brand name: SKICON 200EX, IBS Co., Ltd. make).

From the average value of the measured values, the amount of increase in water content was calculated by the following formula.
Increase amount [μS] = (Average value of water content after 5 minutes of application) − (Average value of water content immediately before application)
The calculated increase amount is shown in the “Moisture retention” column of Table 3 as an evaluation value of the moisture retention.

From the results of the moisture retention shown in Table 3, it can be seen that the cosmetic of Example 1 is more advantageous than the cosmetics of Comparative Examples 1 and 2.

(Examples 2 and 3)
In Examples 2 and 3, cosmetics were prepared in the same manner as in Example 1 except that the mass ratio of the heat-denatured collagen was changed as shown in Table 4.

<Sensory evaluation by panelists>
About the cosmetics of Examples 1 to 3 and Comparative Examples 1 and 2, 30 panelists (women of 40 to 59 years old) applied the skin twice a day in the morning and at night. 30 panelists applied cosmetics for 2 weeks, and after 2 weeks, sensory evaluation was performed on the moist and sticky feeling of the skin. The number of panelists who answered that the moist feeling was good was P1, the number of panelists who answered that they did not feel sticky was P2, and the value calculated by “P1 × 2 + P2” was taken as the overall evaluation score.

  The comprehensive evaluation score of each example is shown in the “Comprehensive evaluation” column of Table 4.

From the comprehensive evaluation results shown in Table 4, it can be seen that the cosmetics of Examples 1 to 3 are more advantageous than the cosmetics of Comparative Examples 1 and 2.

Claims (2)

A cosmetic containing a heat-denatured collagen derived from fish,
Thermally denatured tuna-derived collagen obtained by heat-denaturing tuna-derived collagen under a temperature range of 45-55 ° C. and 0.5-2 hours, and Thai-derived collagen at a temperature range of 45-55 ° C., 0.5- A cosmetic comprising a heat-denatured product of Thai-derived collagen heat-denatured under conditions of 2 hours . A skin cosmetic containing a heat-denatured collagen derived from fish,
Thermally denatured tuna-derived collagen obtained by heat-denaturing tuna-derived collagen under a temperature range of 45-55 ° C. and 0.5-2 hours, and Thai-derived collagen at a temperature range of 45-55 ° C., 0.5- A skin cosmetic characterized by containing a heat-denatured collagen derived from Thailand that has been heat-denatured under conditions of 2 hours .