JP5717977B2 - シアン化合物酸化分解能を有する微生物の検出方法 - Google Patents
シアン化合物酸化分解能を有する微生物の検出方法 Download PDFInfo
- Publication number
- JP5717977B2 JP5717977B2 JP2010092456A JP2010092456A JP5717977B2 JP 5717977 B2 JP5717977 B2 JP 5717977B2 JP 2010092456 A JP2010092456 A JP 2010092456A JP 2010092456 A JP2010092456 A JP 2010092456A JP 5717977 B2 JP5717977 B2 JP 5717977B2
- Authority
- JP
- Japan
- Prior art keywords
- cyanide
- primer
- pcr
- seq
- microorganisms
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000034 method Methods 0.000 title claims description 54
- 244000005700 microbiome Species 0.000 title claims description 47
- XFXPMWWXUTWYJX-UHFFFAOYSA-N Cyanide Chemical compound N#[C-] XFXPMWWXUTWYJX-UHFFFAOYSA-N 0.000 title claims description 26
- 230000003647 oxidation Effects 0.000 title claims description 12
- 238000007254 oxidation reaction Methods 0.000 title claims description 12
- 238000003752 polymerase chain reaction Methods 0.000 claims description 30
- 108010063804 cyanide dihydratase Proteins 0.000 claims description 28
- 230000000692 anti-sense effect Effects 0.000 claims description 20
- 229930010555 Inosine Natural products 0.000 claims description 9
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 claims description 9
- 229960003786 inosine Drugs 0.000 claims description 9
- 230000003321 amplification Effects 0.000 claims description 8
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 8
- 238000003753 real-time PCR Methods 0.000 claims description 8
- 230000007613 environmental effect Effects 0.000 claims description 5
- 108020004414 DNA Proteins 0.000 description 25
- 238000006243 chemical reaction Methods 0.000 description 20
- 238000001514 detection method Methods 0.000 description 18
- 239000012634 fragment Substances 0.000 description 15
- 238000000746 purification Methods 0.000 description 13
- 108090000623 proteins and genes Proteins 0.000 description 12
- 239000000126 substance Substances 0.000 description 12
- 125000003275 alpha amino acid group Chemical group 0.000 description 11
- 239000002689 soil Substances 0.000 description 10
- 239000000523 sample Substances 0.000 description 9
- 238000012544 monitoring process Methods 0.000 description 8
- 238000000137 annealing Methods 0.000 description 6
- 238000001962 electrophoresis Methods 0.000 description 6
- 230000007246 mechanism Effects 0.000 description 6
- 108020004465 16S ribosomal RNA Proteins 0.000 description 5
- 108091034117 Oligonucleotide Proteins 0.000 description 5
- 238000004925 denaturation Methods 0.000 description 5
- 230000036425 denaturation Effects 0.000 description 5
- 238000000605 extraction Methods 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 4
- 238000012408 PCR amplification Methods 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 230000000295 complement effect Effects 0.000 description 4
- 239000003673 groundwater Substances 0.000 description 4
- 150000002825 nitriles Chemical class 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 3
- 210000004899 c-terminal region Anatomy 0.000 description 3
- 238000011109 contamination Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000003344 environmental pollutant Substances 0.000 description 3
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 3
- 229960005542 ethidium bromide Drugs 0.000 description 3
- 238000009396 hybridization Methods 0.000 description 3
- LELOWRISYMNNSU-UHFFFAOYSA-N hydrogen cyanide Chemical compound N#C LELOWRISYMNNSU-UHFFFAOYSA-N 0.000 description 3
- 238000002372 labelling Methods 0.000 description 3
- 231100000719 pollutant Toxicity 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 241000894007 species Species 0.000 description 3
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- 230000004544 DNA amplification Effects 0.000 description 2
- 238000007400 DNA extraction Methods 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- 241000223218 Fusarium Species 0.000 description 2
- 241000589516 Pseudomonas Species 0.000 description 2
- 241001667667 Pseudomonas fluorescens NCIMB 11764 Species 0.000 description 2
- 241000204652 Thermotoga Species 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 239000011616 biotin Substances 0.000 description 2
- 229960002685 biotin Drugs 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- 238000002493 microarray Methods 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 238000010525 oxidative degradation reaction Methods 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 230000000241 respiratory effect Effects 0.000 description 2
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 2
- 239000010865 sewage Substances 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 239000002351 wastewater Substances 0.000 description 2
- 238000004065 wastewater treatment Methods 0.000 description 2
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- 241000588986 Alcaligenes Species 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 241000194103 Bacillus pumilus Species 0.000 description 1
- 108020004705 Codon Proteins 0.000 description 1
- 101000807980 Dehalococcoides mccartyi (strain VS) Chloroethene reductive dehalogenase Proteins 0.000 description 1
- SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 description 1
- 108010028143 Dioxygenases Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000223247 Gloeocercospora Species 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
- 238000009004 PCR Kit Methods 0.000 description 1
- 239000012807 PCR reagent Substances 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 239000008049 TAE buffer Substances 0.000 description 1
- HGEVZDLYZYVYHD-UHFFFAOYSA-N acetic acid;2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid Chemical compound CC(O)=O.OCC(N)(CO)CO.OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O HGEVZDLYZYVYHD-UHFFFAOYSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000027455 binding Effects 0.000 description 1
- 238000010876 biochemical test Methods 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- -1 cyanide compound Chemical class 0.000 description 1
- 125000001295 dansyl group Chemical group [H]C1=C([H])C(N(C([H])([H])[H])C([H])([H])[H])=C2C([H])=C([H])C([H])=C(C2=C1[H])S(*)(=O)=O 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 description 1
- SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 description 1
- 239000003657 drainage water Substances 0.000 description 1
- MLIKRVBXLNTXKF-UHFFFAOYSA-N ethoxyphosphonamidous acid Chemical compound CCOP(N)O MLIKRVBXLNTXKF-UHFFFAOYSA-N 0.000 description 1
- ZFKJVJIDPQDDFY-UHFFFAOYSA-N fluorescamine Chemical compound C12=CC=CC=C2C(=O)OC1(C1=O)OC=C1C1=CC=CC=C1 ZFKJVJIDPQDDFY-UHFFFAOYSA-N 0.000 description 1
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 125000002485 formyl group Chemical class [H]C(*)=O 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000000383 hazardous chemical Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 239000002751 oligonucleotide probe Substances 0.000 description 1
- 238000002515 oligonucleotide synthesis Methods 0.000 description 1
- 238000011022 operating instruction Methods 0.000 description 1
- 238000006864 oxidative decomposition reaction Methods 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 150000004713 phosphodiesters Chemical class 0.000 description 1
- 229920000729 poly(L-lysine) polymer Polymers 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 125000003698 tetramethyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 238000009834 vaporization Methods 0.000 description 1
- 230000008016 vaporization Effects 0.000 description 1
Images
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
(1)シアン化合物酸化分解能を有する微生物の検出方法であって、環境試料より抽出したDNAを鋳型とし、以下のプライマー:
(a)センスプライマー
5’-TT(T/C/A/G/I)CC(T/C/A/G/I)GA(A/G/I)G(T/C/I)(T/C/A/G/I)T(T/G/I)
(T/C/G/I)(T/C/A/I)T(T/C/A/G/I)CC(T/C/A/G/I)GG(T/C/A/G/I) (T/A/I)(C/A/I)(T/C/A/G/I)CC(T/C/A/G/I)-3’(配列番号1)
(b)アンチセンスプライマー
5’-(T/C/A/G/I)GGCCA(T/C/A/G/I)(C/G/I)(T/C/A/I)(T/C/A/G/I)GC
(T/C/A/G/I)AC(A/G/I)TG(T/C/A/G/I)AC(T/C/I)TG(T/C/A/G/I)(T/C/I)C-3’(配列番号2)
(ここで、Iはイノシンを表す。)
を用いてポリメラーゼ連鎖反応(PCR)又はリアルタイムPCRを行い、シアニドジヒドラターゼ遺伝子の増幅産物を検出することを含む、上記方法。
(2) 前記増幅産物を、電気泳動によって検出する、(1)に記載の方法。
(3) 前記センスプライマーが、以下の塩基配列:
5’-TTYCCIGARGCITTYYTICCIGGITAYCCI-3’(配列番号3)
(ここで、Iはイノシンを表す。)
からなる、(1)又は(2)に記載の方法。
(4)前記アンチセンスプライマーが、以下の塩基配列:
5’-GGCCAIGCIGCIACRTGIACYTGYTC-3’(配列番号4)
(ここで、Iはイノシンを表す。)
からなる、(1)又は(2)に記載の方法。
(5) 上記増幅産物の塩基配列を決定することをさらに含む、(1)〜(4)のいずれかに記載の方法。
(6) 決定された塩基配列に基づいて微生物を同定することをさらに含む、(5)に記載の方法。
(8) シアニドジヒドラターゼ遺伝子の検出を、前記塩基配列又はその15塩基長以上の断片が担体上に固定化されたマイクロアレイを用いて実施する、(7)に記載の方法。
(9) 前記検出を、PCR、リアルタイムPCR又はハイブリダイゼーションによって実施する、(7)に記載の方法。
(10)シアン化合物酸化分解能を有する微生物を検出するためのプライマーセットであって、以下のプライマー:
(a)センスプライマー
5’-TT(T/C/A/G/I)CC(T/C/A/G/I)GA(A/G/I)G(T/C/I)(T/C/A/G/I)T(T/G/I)
(T/C/G/I)(T/C/A/I)T(T/C/A/G/I)CC(T/C/A/G/I)GG(T/C/A/G/I) (T/A/I)(C/A/I)(T/C/A/G/I)CC(T/C/A/G/I)-3’(配列番号1)
(b)アンチセンスプライマー
5’-(T/C/A/G/I)GGCCA(T/C/A/G/I)(C/G/I)(T/C/A/I)(T/C/A/G/I)GC
(T/C/A/G/I)AC(A/G/I)TG(T/C/A/G/I)AC(T/C/I)TG(T/C/A/G/I)(T/C/I)C-3’(配列番号2)
(ここで、Iはイノシンを表す。)
を含む、上記プライマーセット。
(11) 前記センスプライマーが、以下の塩基配列:
5’-TTYCCIGARGCITTYYTICCIGGITAYCCI-3’(配列番号3)
(ここで、Iはイノシンを表す。)
からなる、(10)に記載のプライマーセット。
(12) 前記アンチセンスプライマーが、以下の塩基配列:
5’-GGCCAIGCIGCIACRTGIACYTGYTC-3’(配列番号4)
(ここで、Iはイノシンを表す。)
からなる、(10)に記載のプライマーセット。
HCN+2H2O+O2→2NH3+2CO2+2H2(酸化分解)
シアン化合物に対して分解能力を持つPseudomonas fluorescens NCIMB 11764株のゲノムDNAにおいて、図2に示すセンスプライマーの配列を基にして作成したFwプライマー(5’‐TTYCCIGARGCITTYYTICCIGGITAYCCI‐3’:配列番号3)と、図3に示すアンチセンスプライマーの配列を基にして作成したRvプライマー(5’−GGCCAIGCIGCIACRTGIACYTGYTC‐3’:配列番号4)を用いてシアニドジヒドラターゼ遺伝子のPCR増幅試験を行った。
Claims (2)
- シアン化合物酸化分解能を有する微生物の検出方法であって、環境試料より抽出したDNAを鋳型とし、以下のプライマー:
(a)センスプライマー
5’-TTYCCIGARGCITTYYTICCIGGITAYCCI-3’(配列番号3)
(b)アンチセンスプライマー
5’-GGCCAIGCIGCIACRTGIACYTGYTC-3’(配列番号4)
(ここで、Iはイノシンを表す。)
を用いてポリメラーゼ連鎖反応(PCR)又はリアルタイムPCRを行い、シアニドジヒドラターゼ遺伝子の増幅産物を検出することを含む、上記方法。 - シアン化合物酸化分解能を有する微生物を検出するためのプライマーセットであって、以下のプライマー:
(a)センスプライマー
5’-TTYCCIGARGCITTYYTICCIGGITAYCCI-3’(配列番号3)
(b)アンチセンスプライマー
5’-GGCCAIGCIGCIACRTGIACYTGYTC-3’(配列番号4)
(ここで、Iはイノシンを表す。)
を含む、上記プライマーセット。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2010092456A JP5717977B2 (ja) | 2010-04-13 | 2010-04-13 | シアン化合物酸化分解能を有する微生物の検出方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2010092456A JP5717977B2 (ja) | 2010-04-13 | 2010-04-13 | シアン化合物酸化分解能を有する微生物の検出方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2011217695A JP2011217695A (ja) | 2011-11-04 |
JP5717977B2 true JP5717977B2 (ja) | 2015-05-13 |
Family
ID=45035466
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2010092456A Active JP5717977B2 (ja) | 2010-04-13 | 2010-04-13 | シアン化合物酸化分解能を有する微生物の検出方法 |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP5717977B2 (ja) |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP5237576B2 (ja) * | 2007-05-16 | 2013-07-17 | 大成建設株式会社 | シアン化合物分解能を有する微生物の検出及び同定方法 |
-
2010
- 2010-04-13 JP JP2010092456A patent/JP5717977B2/ja active Active
Also Published As
Publication number | Publication date |
---|---|
JP2011217695A (ja) | 2011-11-04 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Horz et al. | Identification of major subgroups of ammonia-oxidizing bacteria in environmental samples by T-RFLP analysis of amoA PCR products | |
Ward et al. | Analysis of ammonia-oxidizing bacteria from hypersaline Mono Lake, California, on the basis of 16S rRNA sequences | |
Táncsics et al. | Investigation of catechol 2, 3-dioxygenase and 16S rRNA gene diversity in hypoxic, petroleum hydrocarbon contaminated groundwater | |
JP4836552B2 (ja) | 実汚染土壌を効率よく浄化する微生物および浄化方法 | |
Chikere | Culture-independent analysis of bacterial community composition during bioremediation of crude oil-polluted soil. | |
Silva et al. | Investigation of bacterial diversity in membrane bioreactor and conventional activated sludge processes from petroleum refineries using phylogenetic and statistical approaches | |
Oshiki et al. | N2O reduction by Gemmatimonas aurantiaca and potential involvement of Gemmatimonadetes bacteria in N2O reduction in agricultural soils | |
US20090258349A1 (en) | Method for detecting and quantifying perchlorate-reducing bacteria | |
JP5237576B2 (ja) | シアン化合物分解能を有する微生物の検出及び同定方法 | |
JP5717977B2 (ja) | シアン化合物酸化分解能を有する微生物の検出方法 | |
JP2019054735A (ja) | シアン分解能を有する微生物の選択方法、シアン分解能を有する微生物、及びその用途 | |
Şentürk et al. | Ammonia-Oxidizing Bacteria: Biochemical and Molecular Characteristics | |
US20090068650A1 (en) | Metabolic Primers for the Detection of (Per) Chlorate-Reducing Bacteria and Methods of Use Thereof | |
JP4511980B2 (ja) | T−rflp法による硝化活性能力の測定方法 | |
McMahon et al. | Molecular methods in biological systems | |
JP5779440B2 (ja) | ベンゼン分解菌の検出方法 | |
JP2005218322A (ja) | 塩素化エタン分解菌検出用核酸断片、検出方法および塩素化エタン分解方法 | |
JP4857654B2 (ja) | 塩化ビニル分解能を有する微生物の検出方法 | |
Mattes et al. | Quantifying the presence and activity of aerobic, vinyl chloride‐degrading microorganisms in dilute groundwater plumes by using real‐time PCR | |
JP3861140B2 (ja) | 複合生物系試料における硫酸還元菌を特異的に検出するためのオリゴクレオチドプライマー | |
Kirisits et al. | Prokaryotic cDNA Subtraction: A Method to Rapidly Identify Functional Gene Biomarkers | |
CA2383745A1 (en) | Nucleic acids for detecting dechlorination bacteria and uses thereof | |
KR20060064271A (ko) | 혐기소화공정의 메탄생성균에 대한 정량방법 | |
JP2004283032A (ja) | 核酸断片、アナモックス細菌検出用核酸断片、プライマー、プローブ、アナモックス細菌の検出方法、およびアンモニア性窒素含有排液の処理方法 | |
JP2004261126A (ja) | 環境試料のアルカン分解能の評価法及びそのためのオリゴヌクレオチド |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20120620 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20140114 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20140314 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20140924 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20141120 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20150224 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20150318 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 5717977 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |