JP5717433B2 - Bile acid adsorption composition - Google Patents

Description

  The present invention relates to a composition that can adsorb bile acids and promote their excretion.

In recent years, the intake of fat has increased due to the westernization of food, and the number of cancer patients has increased. Examples of cancers that are highly correlated with ingested fat include breast cancer and colon cancer. In particular, colorectal cancer has rapidly increased in number of patients, and the number of deaths as colorectal cancer combining the colon and rectum is the third highest after lung cancer and stomach cancer. As a cause of colorectal cancer, the amount of bile acids increases due to fat intake. Along with this, the metabolism of bile acids such as steroid metabolites and secondary bile acids has the effect of intestinal mucosal damage and tumor promoters. This is thought to increase the product.
On the other hand, cholelithiasis is a disease caused by crystallization of bile acid or the pigment bilirubin, but cholelithiasis is increasing with the increase in fat intake due to westernization of food. Bile acid metabolism is also considered to be involved in the development of cholelithiasis.

Therefore, in order to prevent or treat these diseases, bile acid adsorbing materials for adsorbing bile acids and promoting their excretion outside the body have already been reported.
For example, Patent Document 1 describes that a bile acid adsorbent and a medicine containing an anion resin are conventionally known. However, since these are synthetic compounds, strict attention is required for their use from the viewpoint of safety, and in the case of pharmaceuticals, the availability is limited, making them unsuitable for use in normal life. It is.

  In addition, as a food-derived material, there are reports that dietary fibers and indigestible proteins adsorb bile acids, and the bile acid adsorption action of foods that contain a lot of dietary fibers such as bran that have been discarded in the past has attracted attention. Yes. However, many dietary fibers have a very bad flavor and are not suitable for consumption, and there is a problem that the effect cannot be obtained unless a large amount is consumed.

  As another food-derived material, Patent Document 2 discloses that it is produced by a filter residue obtained by filtering a solution containing a fish clause that has been enzymatically decomposed with an endo-exo-type protease derived from microorganisms, and has a bile acid adsorption ability. A bile acid adsorbent characterized by is described. Patent Document 3 describes a bile acid adsorbent characterized by containing chitosan orotate as an active ingredient. However, the effect of these conventional bile acid adsorbents is not always satisfactory.

  Therefore, in reality, there is a demand for a bile acid-adsorbing material that is excellent in bile acid-adsorbing ability and that can be ingested safely and continuously.

Japanese Patent Laid-Open No. 7-277993 Japanese Patent No. 4353746 JP 2004-51615 A

  The present invention has been made in view of such conventional problems and circumstances, and has an object to provide a material that has an action of adsorbing bile acids, is easily ingested as food, and is highly safe for a living body.

  The present inventor has found that the water-soluble component obtained from the degradation product of the proteolytic enzyme of the fibrous protein has an excellent bile acid adsorption ability as a result of intensive studies to solve the above problems, The present invention has been completed.

That is, the present invention provides a bile acid adsorption composition containing a water-soluble component of a degradation product of a fibrous protein by a proteolytic enzyme or a degradation product of a proteolytic enzyme and a glycolytic enzyme. It solves the problem.
The present invention also provides a method for adsorbing bile acids, characterized by degrading a fibrous protein with a proteolytic enzyme, or decomposing with a proteolytic enzyme and a glycolytic enzyme, and obtaining a water-soluble component from the obtained degradation product. The above-described problems are solved by providing a method for producing a composition.
Moreover, this invention solves the said subject by providing the pharmaceutical, food-drinks, and feed containing the said composition for bile acid adsorption | suction.

  The composition for adsorbing bile acids according to the present invention has high bile acid adsorbing ability and has high safety without causing side effects even when taken for a long time. Therefore, the composition for adsorbing bile acids according to the present invention can be used as a medicine, food and drink, and feed for adsorbing bile acids and promoting their excretion, or for the development of colorectal cancer and gallstones. It can be used as a medicine, food and drink, and feed for preventing and / or treating a disease associated with the metabolism of.

The graph which shows the bile acid adsorption ability of each test substance.

The composition for adsorbing bile acids of the present invention is effective in using a water-soluble component of a degradation product of a fibrous protein by a proteolytic enzyme, preferably a water-soluble component of a degradation product of a fibrous protein by a proteolytic enzyme and a glycolytic enzyme. Contains as a component. The composition for adsorbing bile acids of the present invention is substantially composed of a water-soluble component of a degradation product of a fibrous protein by a proteolytic enzyme, preferably a water-soluble component of a degradation product of a fibrous protein by a proteolytic enzyme and a glycolytic enzyme. Including those configured.
In the present invention, the fibrous protein refers to a protein having a fibrous or network structure as a tertiary structure or a quaternary structure. Fibrous proteins include animal-derived proteins such as actin and myosin contained in muscle, collagen contained in skin, gluten and gliadin contained in wheat, caffeine contained in oyster millet, zein contained in corn, etc. And proteins derived from microorganisms such as polyglutamine produced by Bacillus natto. Among these, gluten or kafilin is preferable from the viewpoint of availability, safety, desired activity, flavor, and the like.

  Gluten is a protein known to be abundant in wheat seeds. In the present invention, purified gluten may be used as gluten, but wheat seed-derived protein (wheat protein) itself may be used. For example, since gluten is usually contained in wheat protein in an amount of about 80 to 90%, the composition of the present invention can be obtained using wheat protein as a raw material. The gluten that can be used for the present invention may be gluten or protein appropriately prepared from wheat, or commercially available gluten or wheat protein.

  Kafilin is a protein that is known to be contained in large amounts in the seeds of oyster millet. In the present invention, commercially available or appropriately prepared purified kaffirin can be used as the kafilin. Alternatively, the composition of the present invention can also be obtained by crushing or pulverizing the seeds of the millet as necessary, and then decomposing it with a proteolytic enzyme.

The fibrous protein is decomposed with a proteolytic enzyme, preferably with a proteolytic enzyme and a glycolytic enzyme, and a water-soluble component is obtained from the resulting decomposition product to produce the composition for adsorbing bile acids according to the present invention. can do.
The origin of the proteolytic enzyme is not particularly limited, and proteases derived from any organism such as animals, plants, microorganisms and the like can be used, and one or more of these can be used. Examples of the proteolytic enzymes include, for example, plant-derived rhizomes such as papaya and pineapple, protease extracted and purified from fruit juice, microorganisms-derived Aspergillus genus, Rhizopus genus, Streptomyces genus, Penicillium genus, Bacillus genus, etc. Examples include proteases extracted and purified from psins, and those derived from animals such as pepsin extracted from porcine pancreas and the like, and proteases such as neurase and chymotrypsin. Among these, a proteolytic enzyme having endopeptidase activity that cleaves from the center of the amino acid chain of the protein is preferable, and a proteolytic enzyme that acts in the vicinity of acidity is more preferable. Examples of proteolytic enzymes that act in the vicinity of acidity include cysteine proteases and aspartic proteases, and specific examples include papain, proteases derived from the genus Aspergillus, pepsin, cathepsin, and nuclease. Examples of the proteolytic enzymes include commercially available proteolytic enzymes such as: Protease M Amano (acidic protease derived from microorganisms of the genus Aspergillus, Amano Enzyme Inc.); Newase (Neurase F3G, Amano Enzyme Inc.); Orientase 20A (protease derived from microorganisms belonging to the genus Aspergillus, HTV Inc.); orientase 90N (protease derived from microorganisms belonging to the genus Bacillus, HTVii Inc.); orientase ONS (genus Aspergillus) Protease derived from microorganisms, HIBI Co., Ltd.), Neurase F3G (acidic protease derived from microorganisms belonging to the genus Rhizopus, Amano Enzyme Inc.); Protease A Amano G (proteases derived from microorganisms belonging to the genus Aspergillus, Amano En Im Corp.); Protease N Amano G (Protease derived from microorganisms belonging to the genus Bacillus, Amano Enzyme Inc.); Sumiteam AP (acidic protease derived from microorganisms belonging to the genus Aspergillus, Shinnippon Chemical Co., Ltd.); A microorganism-derived protease, Shin Nippon Chemical Industry Co., Ltd.) and the like can also be used.
Among the proteolytic enzymes, pepsin, papain, neurase, and acidic protease derived from microorganisms belonging to the genus Aspergillus are preferable.
These proteolytic enzymes may be used alone or in appropriate combination.

When the fibrous protein is derived from a plant or a bacterium, it is preferable to perform a decomposition treatment with a glycolytic enzyme in addition to the decomposition with a proteolytic enzyme. Impurities such as starch and fiber that are inevitably mixed from the source of fibrous protein are decomposed by the glycolytic enzyme, so that the active ingredient can be obtained in high yield.
The origin of the saccharolytic enzyme is not particularly limited, and it may be derived from any organism such as an animal, plant, or microorganism. Examples of the glycolytic enzyme include amylases such as α-amylase and glucoamylase; glucosidases such as α-glucosidase and transglucosidase; pullulanase; isoamylase; pancreatin; Alternatively, commercially available glycolytic enzymes, for example: Liquefaction enzyme T: (α-amylase derived from microorganisms belonging to the genus Bacillus, HTV Corporation); Gluc SG (glucoamylase derived from microorganisms belonging to the genus Rhizopus, Amano Enzyme Corporation); (Glucoamylase derived from microorganisms belonging to the genus Rhizopus, Amano Enzyme Co., Ltd.); Cochase (alpha-amylase derived from microorganisms belonging to the genus Aspergillus, manufactured by Mitsubishi Chemical Foods); Spitase CP-40FG (alpha-amylase derived from microorganisms belonging to the genus Bacillus, Nagase ChemteX Corporation); Sumiteam AS (alpha-amylase derived from microorganisms of the genus Aspergillus, Shinnihon Chemical Industry Co., Ltd.); Sumiteam AC (cellulase derived from microorganisms of the genus Aspergillus, Shinnihon Chemical Industry Co., Ltd.); Hemicellulase Amano 90 (Hemicellulase from Aspergillus sp. This Chemical Industry Co., Ltd.) can also be used.
Among the above glycolytic enzymes, amylase, particularly α-amylase, cellulase or pancreatin derived from microorganisms belonging to the genus Bacillus is preferable.
These glycolytic enzymes may be used alone or in appropriate combination.
The treatment with the glycolytic enzyme may be performed before or after the treatment with the proteolytic enzyme, or may be performed in parallel with the treatment with the proteolytic enzyme.
The combination of the glycolytic enzyme and the proteolytic enzyme is not particularly limited. For example, a combination of amylase or liquefying enzyme T and pepsin, papain, neurase, or an acidic protease derived from a microorganism belonging to the genus Aspergillus, or pancreatin and pepsin. A combination with papain or an acidic protease derived from a microorganism belonging to the genus Aspergillus is preferred.

The proteolytic enzyme is allowed to act on the fibrous protein, or the proteolytic enzyme and the glycolytic enzyme are allowed to act simultaneously or sequentially to prepare an enzymatic degradation product of the fibrous protein. At that time, the enzyme may be immobilized on a carrier, and the enzyme may act on the fibrous protein by contacting the carrier with a solution in which the fibrous protein is dissolved or dispersed. Both enzymes may be dissolved or dispersed to allow the enzyme to act on the fibrous protein.
Examples of the medium for dissolving or dispersing the fibrous protein include aqueous liquids such as pure water, distilled water, tap water, acidic water, alkaline water, neutral water, electrolytic water, and buffer solutions such as phosphate buffers. The conditions such as the type, temperature and pH are appropriately determined according to the environment such as the production environment and the properties of the fibrous protein used. The enzyme reaction conditions (temperature, pH, time, etc.) are appropriately determined in consideration of the amount of the substrate, the optimum conditions for the enzyme used, the optimum conditions for the enzyme used simultaneously, and the like.
The reaction is preferably allowed to proceed until the maximum amount of water-soluble degradation product is obtained by the enzyme. If the protein degradation is incomplete, many insoluble residues remain, resulting in a decrease in the yield of the composition of the present invention.
In order to complete the enzymatic degradation reaction, the carrier may be removed from the solution when the enzyme is immobilized on the carrier, and the pH-optimized enzyme is optimal when the enzyme is dissolved or dispersed. Known methods such as a method of largely dissociating from pH and a method of inactivating the enzyme by raising the temperature of the solution can be appropriately applied.

Next, the degradation product obtained by the enzyme treatment can be separated from the degradation product by subjecting it to any means such as drying, centrifugation, filtration, and distribution.
In the present invention, the water-soluble component is a temperature of 5 to 80 ° C., preferably 8 to 65 ° C., more preferably around 15 to 50 ° C., and a pH of 2 to 11, preferably 3 to 10, from the decomposition product. More preferably, it refers to a component capable of dissolving or forming a colloid in an aqueous liquid at around 4-8. Examples of the aqueous liquid include pure water, distilled water, tap water, acidic water, alkaline water, neutral water, and buffer solutions such as phosphate buffer solutions.
Usually, if the enzymatic degradation of the fibrous protein is sufficiently performed, the components in the water-soluble component of the present invention will not change even if the temperature or pH changes somewhat. Therefore, when the water-soluble component is separated from the decomposition product, the decomposition product may be subjected to a separation operation as it is, or may be subjected to conditions such as a temperature at which the separation operation is easily performed.
For example, a solution of a degradation product obtained by treating the above fibrous protein solution with a proteolytic enzyme is 2500 to 30000 g, preferably 3500 to 23000 g, more preferably 4500 to 18000 g, and further preferably 5000 to 15000 g. The water-soluble component can be separated from the decomposition product by centrifuging for 10 to 60 minutes, preferably 20 minutes, and collecting the supernatant.

By recovering the water-soluble component separated by the above means, the water-soluble component of the degradation product of the fibrous protein by the proteolytic enzyme, preferably the water-soluble component of the degradation product by the proteolytic enzyme and the glycolytic enzyme Can be obtained.
Alternatively, examples of the water-soluble components include water-soluble components of wheat gluten protease and amylase degradation product obtained by the method for producing a glutamine peptide described as Production Example 1 in International Publication No. WO03 / 074071. .
The water-soluble component may be further subjected to treatments such as dilution, concentration, drying, freeze-drying, solidification, liquefaction, granulation, or powdering as necessary.

  Water-soluble component of the degradation product of the above-mentioned fibrous protein by proteolytic enzyme, or water-soluble component of the degradation product of proteolytic enzyme and glycolytic enzyme (collectively referred to as water-soluble component of the enzymatic degradation product of fibrous protein) The composition for adsorbing bile acids according to the present invention, which contains, as shown in the Examples below, has a significantly superior bile acid adsorption ability compared to dietary fibers that are conventionally known to have a bile acid adsorption effect. . Therefore, the composition of the present invention can exert an excellent effect on the adsorption and excretion of bile acids, or can prevent and / or prevent diseases such as colorectal cancer and gallstones that involve bile acid metabolism. It can exert an excellent effect on treatment.

The above-described fibrous proteins, such as gluten and caffeine, which are used as raw materials for the composition for adsorbing bile acids according to the present invention, are substances that have been used as food materials in the past, and there are no side effects even if they are taken for a long time. Therefore, it can be safely used not only for healthy persons and adults but also for children, the elderly and the sick. Therefore, the composition for adsorbing bile acids of the present invention can be used as a medicine for humans or animals, food or drink, feed, or the like, or for producing them.
Therefore, this invention also provides the pharmaceutical, food-drinks, and feed containing the said composition for bile acid adsorption | suction of this invention.

The medicament of the present invention can be a bile acid adsorbent containing the bile acid adsorption composition of the present invention as an active ingredient.
The medicament can be administered in any dosage form including oral and parenteral administration. Dosage forms for oral administration include solid preparations such as tablets, coated tablets, granules, powders, capsules, or liquid preparations such as elixirol, syrups and suspensions. Examples of the dosage form for parenteral administration include injection, infusion, transdermal, transmucosal, nasal, enteral, inhalation, suppository, bolus and the like.

The food / beverage product or feed of the present invention contains the composition for adsorbing bile acids of the present invention as an active ingredient, and is intended for the effect of adsorbing bile acids, and is a functional food / beverage product or food / beverage for the sick. Product, food and drink for specified health use, livestock feed, pet food and the like.
Although the form of the said food / beverage products or feed is not specifically limited, For example, as a drink form, tea drink, coffee drink, milk drink, fruit juice drink, carbonated drink, alcoholic drink, soft drink etc. are mentioned, food or feed The form may be solid, semi-solid or liquid and includes tablet form, pill form, capsule form, liquid form, syrup form, powder form, granule form and the like. Specific food forms include breads, noodles, jelly-like foods and various snacks, baked goods, cakes, chocolate, gum, candy, tablets, capsules, soups, dairy products, frozen foods, instant foods, Examples include supplements, other processed foods, seasonings, and materials thereof.

The bile acid-adsorbing composition, medicine, food / drink or feed of the present invention, if necessary, other components other than the water-soluble component of the enzymatic degradation product of the fibrous protein, such as other active ingredients and / or It may contain commonly used additives or carriers.
In the case of medicine, the other ingredients include, for example, other medicinal ingredients, cosmetic ingredients, nutrition ingredients, and excipients, disintegrants, binders, lubricants, surfactants, water-soluble polymers, diluents. , Osmotic pressure adjusting agent, pH adjusting agent, emulsifier, preservative, buffer, stabilizer, antioxidant, thickener, ultraviolet absorber, activity enhancer, colorant, sweetener, flavoring agent, flavoring agent, A sour agent etc. are mentioned, These are used in combination suitably.
In the case of food and drink or feed, examples of the other ingredients include other food and drink materials, nutritional ingredients, cosmetic ingredients, and solvents, oils, softeners, emulsifiers, preservatives, stabilizers, antioxidants, and coloring agents. , Ultraviolet absorbers, moisturizers, thickeners, brighteners, sweeteners, fragrances and the like, and these are used in appropriate combinations.

In the composition of the present invention, medicine, food or drink or feed, the content of the water-soluble component of the enzymatic degradation product of the fibrous protein varies depending on the dosage form and form, but in terms of dry solids, In the case of a pharmaceutical, it is 1-90 mass% with respect to the total mass, Preferably it is 5-70 mass%, and in the case of food-drinks or feed, it is 5-100 mass%, Preferably it is 10-90 mass%.
The administration or intake of the composition for adsorbing bile acids according to the present invention is usually 2 g to 30 g, preferably 6 to 30 g per day for an adult in terms of dry solid content of the water-soluble component of the enzymatic degradation product of the fibrous protein. It is. The administration or intake amount can be appropriately adjusted according to the usage and application target.

  EXAMPLES Next, although an Example is shown and this invention is demonstrated further in detail, this invention is not limited to a following example.

Example 1 Preparation of Water-Soluble Component of Enzymatic Degradation Product of Gluten (1) 9,700 L of ion exchange water, 38 kg of anhydrous citric acid and 1,500 kg of wheat gluten (active gluten: George Weston Foods Limited) were charged in a reaction kettle, After heating to 45 ° C., 2.2 kg of protease (Protease M Amano; Amano Enzyme Co., Ltd.) and 1.1 kg of saccharolytic enzyme (Liquefied enzyme T; HBI Co., Ltd.) are added, and water is added at 45 ° C. for 5 hours. The decomposition reaction was carried out, and then the enzyme treatment was carried out by adjusting the pH of the reaction solution to 4.4 to 4.5 using a 25% aqueous sodium hydroxide solution and keeping it for 5 hours.
(2) Next, the reaction solution was kept at 80 ° C. for 20 minutes to inactivate the protease, then cooled to 65 ° C., and 0.5 kg of a saccharide-degrading enzyme (liquefied enzyme T; HTV Corporation) was added to the wheat. After the carbohydrate contained in gluten was hydrolyzed, the liquid was kept at 90 ° C. for 20 minutes to inactivate amylase.
(3) Next, the reaction solution was cooled to 10 ° C. or lower and then heated again to 55 ° C., and 100 kg of activated carbon (“Takecol” manufactured by Takeda Pharmaceutical Co., Ltd.) was added thereto and stirred at 55 ° C. for 30 minutes.
(4) The liquid temperature was set to 45 ° C., a concentrated auxiliary agent (“Radiolite” manufactured by Showa Chemical Industry Co., Ltd.) was added, and filtration was performed using a pressure filtration device, and 7,000 L of the filtrate was recovered.
(5) The filtrate collected in (4) above is concentrated under reduced pressure until the Brix value is 20-40, then sterilized by heating at 110 ° C. for 20 seconds using a plate heater, and then cooled to 55 ° C. did.
(6) The liquid obtained in (5) above is spray-dried using a spray drying device under conditions of an air blowing temperature of 160 ° C. and an exhaust air temperature of 80 ° C. About 1,000 kg was obtained in the form.
(7) The wheat protein hydrolyzate powder obtained in (6) above is classified using a 60 mesh sieve (aperture 0.246 mm), and the fine powder that has passed through the 60 mesh sieve is recovered, and the present invention It was set as the composition.

Example 2 Preparation of Water-Soluble Component of Enzyme Degradation Product of Pepper Millet (1) Peanut seed (GNS Co., Ltd.) is crushed, 10 times (w / v) pure water of crushed product is added, and pH is adjusted with concentrated hydrochloric acid Adjusted to 2.0. To this, pepsin (derived from porcine stomach mucosa: Wako Pure Chemical Industries, Ltd.) having a mass ratio of ground millet seeds of 1% was added and reacted at 37 ° C. for 4 hours. Tris-HCl was added to the reaction solution to a final concentration of 25 mM, and then the pH was adjusted to 8.0 with concentrated sodium hydroxide. Pancreatine (pig pancreas origin: sum) (Optical Pure Chemical Industries, Ltd.) was added and the reaction was allowed to proceed at 37 ° C. for 24 hours.
(2) The reaction solution of (1) was centrifuged at 15,000 G for 20 minutes, and the supernatant was dissolved by adding ammonium sulfate at a saturation degree of 80%, shaken and stirred, and allowed to stand at 4 ° C. for 12 hours.
(3) Next, this was centrifuged at 1,700 G for 15 minutes, and the precipitate was dissolved in 10 mM phosphate buffer. The lysate was placed in a dialysis membrane having a molecular weight of 3,500 cuts, and dialyzed in a 100-fold amount of 10 mM phosphate buffer. The dialysate was lyophilized and recovered as a composition of the present invention.

Test Example 1 Bile acid adsorption test (1) Preparation of bile acid mixed solution 242 mg of taurocholic acid (Wako Pure Chemical Industries) and 658.3 mg of glucocholic acid (CALBIOCHEM) were mixed, and 10 mM phosphate buffer solution (pH = 6.8). ) Dissolved in 20 mL. The volume was made up to 50 mL with 10 mM phosphate buffer (pH = 6.8) to prepare a 36 mM bile acid mixture (stock solution).
(2) Bile acid adsorption 200 mg of the test substance was weighed into a centrifuge tube, and 4 mL of a 50-fold diluted solution of the stock solution prepared in (1) was added thereto. A pancreatin solution (pancreatin (pig pancreas origin: Wako Pure Chemical Industries) 10 mg / mL 100 mM phosphate buffer) was further added thereto, and the mixture was reacted at 37 ° C. for 1 hour. The reaction solution was centrifuged at 100,000 G for 18 minutes, and the supernatant was collected. The precipitate was suspended by adding 5 mL of 100 mM phosphate buffer, centrifuged at 100,000 G for 18 minutes, and the supernatant was collected and combined with the previous supernatant.
(3) Measurement of bile acid adsorption rate The bile acid concentration in the above supernatant was measured by a conventional method using a bile acid measurement kit (Wako Pure Chemical Industries, Ltd., “Total Bile Acid Test Wako”). The rate was determined.

Test substance bile acid adsorption rate (%) =
((Negative control bile acid concentration-bile acid concentration of test substance) / negative control bile acid concentration) × 100

In addition, the following was used as a test substance.
Example 1 Composition of the present invention (water-soluble component of gluten enzyme degradation product)
Example 2 Composition of the Present Invention (Water-Soluble Component of Enzyme Degradation Product of Prickly Millet)
Comparative Example 1 Wheat gluten (active gluten: George Weston Foods Limited)
Comparative example 2 Ground millet seed (GNS Co., Ltd.) Comparative example 3 Insoluble component of proteolytic enzyme degradation product of red millet Positive control Cholestyramine (SIGMA)
Negative control Cellulose (SIGMA)

For reference, a method for producing an insoluble component of the pepsin degradation product of oyster mill of Comparative Example 3 is described below.
Comparative Example 2 Insoluble component of pepsin degradation product of oyster millet Seed millet seed was pulverized, 10 times the amount (w / v) of pure water was added, and the pH was adjusted to 2.0 with concentrated hydrochloric acid. To this, pepsin (derived from porcine stomach mucosa: Wako Pure Chemical Industries, Ltd.) having a mass ratio of ground millet seeds of 1% was added and reacted at 37 ° C. for 4 hours. Tris-HCl was added to the reaction solution to a final concentration of 25 mM, and then the pH was adjusted to 8.0 with concentrated sodium hydroxide. Pancreatine (pig pancreas origin: sum) (Optical Pure Chemical Industries, Ltd.) was added and the reaction was allowed to proceed at 37 ° C. for 24 hours. Next, the reaction solution was centrifuged at 15,000 G for 20 minutes to collect a precipitate.

(4) Results The results are shown in Table 1 below and FIG. It was shown that the composition of the present invention has a high bile acid binding ability as compared with cellulose which is dietary fiber, and gluten and oyster mill which have not been subjected to enzyme treatment, and an insoluble component of oyster mill degradation product. .

Claims (8)

The decomposition product by the 蛋 white enzymes and glycolytic enzymes fibrous protein, a bile acid composition containing a water-soluble component,
The proteolytic enzyme is at least one selected from pepsin, papain, neurase, acidic protease derived from microorganisms of the genus Aspergillus,
The glycolytic enzyme is amylase and / or pancreatin,
A composition for adsorbing bile acids .   The composition according to claim 1, wherein the fibrous protein is derived from cereal grains.   The composition according to claim 1, wherein the fibrous protein is gluten or kafilin. The composition according to claim 1, wherein the fibrous protein is a protein prepared from wheat or a millet seed. The fibrous protein was degraded in the 蛋 white enzymes and glycolytic enzymes, a process for the preparation of the resulting decomposed product bile acid composition characterized in that to obtain the water-soluble components from,
The proteolytic enzyme is at least one selected from pepsin, papain, neurase, acidic protease derived from microorganisms of the genus Aspergillus,
The glycolytic enzyme is amylase and / or pancreatin,
Way . The method according to claim 5 , wherein the fibrous protein is derived from cereal grains. 6. The method according to claim 5 , wherein the fibrous protein is gluten or kafilin. The composition according to claim 5, wherein the fibrous protein is a protein prepared from wheat or oyster millet seeds.