JP5604144B2 - Sample component analyzer - Google Patents

Description

  The present invention relates to a sample component analyzer that takes a sample collected in a syringe into the apparatus from the inside of the syringe and analyzes the components of the taken sample.

2. Description of the Related Art Conventionally, there is a sample component analyzer that collects a sample (typically blood) with a syringe, takes the sample collected with the syringe into the apparatus, and analyzes the components of the taken sample.
In such a sample component analyzer, methods for taking a sample into the device include a method for pushing the sample from the syringe into the device and a method for sucking the sample in the syringe from the device side.
A device that employs the former method branches the flow path through which the sample pushed from the syringe flows, provides a sensor unit for component analysis on one branch path, and connects the other branch path directly to the drainage section. However, when the sample is pushed too far from the syringe, the excess sample flows to the branch path connected to the drainage unit, and a certain amount of sample is introduced to the sensor unit without being affected by fluctuations in the push amount from the syringe. It is configured to be.
However, in the case of the method of pushing the specimen from the syringe into the apparatus, the pushing operation is performed by a human, and therefore, for example, if the operator pushes the specimen too rapidly, the flow path may be clogged. There is a problem that there is. If the sample is forcibly pushed in while the flow path is clogged, there is no possibility of the force to be pushed away and the sample may scatter and the operator may be exposed to the sample.
On the other hand, in the case of the method of sucking the sample in the syringe from the apparatus side, the above-mentioned problems do not occur because the suction force and the suction amount can be controlled on the apparatus side.

However, when the sample in the syringe is aspirated from the apparatus side, there is a problem that it takes time because the sample needs to be aspirated by inserting a nozzle into the syringe.
That is, in the case of the method of pushing the sample from the syringe into the apparatus, the operator may simply connect and push the syringe into the apparatus. However, when the sample in the syringe is aspirated from the apparatus side, the syringe is the apparatus. If it is determined that the syringe is set in the device, it is necessary to move the nozzle to enter the syringe and then aspirate the sample with the nozzle. Extra time is required for processing and moving processing. In addition, since the viscosity of specimens, particularly blood, differs from specimen to specimen, if the suction speed is high, even if suction is performed for the same time with the same suction force, the specimen with a higher viscosity has a lower suction volume than the specimen with a lower viscosity. There is a problem of fewer. If the amount of aspiration varies, the amount of the sample guided to the sensor unit also varies, which is a problem because a stable analysis result cannot be obtained. For this reason, in the conventional analyzer, the sample is aspirated at a suction speed that is not affected by the difference in viscosity, that is, at a relatively slow suction speed. However, if the suction speed is limited in this way, the time for taking the sample from the syringe into the apparatus cannot be shortened, resulting in a problem that the analysis speed cannot be increased.
In many cases, it is desirable to shorten the time of component analysis of specimens, especially when blood gas analysis measurement is required, treatment for treatment is required on the order of minutes, in order to obtain treatment guidelines. In some cases, it is required to reduce the time for the inspection performed in this way. Therefore, it is very important to reduce the aspiration time in the method for aspirating the specimen in the syringe from the apparatus side.
An object of the present invention is to solve the above-mentioned conventional problems and to provide a sample component analyzer that can sufficiently shorten the time for taking a sample from a syringe into the device.

In order to achieve the above-described object, a sample component analyzing apparatus according to the present invention includes a syringe mounting portion to which a syringe from which a sample is collected can be mounted, and a syringe inserted into the syringe mounted on the syringe mounting portion. An aspiration nozzle for aspirating the specimen inside, a suction pump connected to the aspiration nozzle, a sensor for analyzing the component of the specimen aspirated by the aspiration nozzle, and a control device for controlling at least the operation of the aspiration pump; Provided with means for measuring the flow rate of the sample in the suction nozzle, and storing the basic suction time according to the sample of standard viscosity in advance in the control device, If the actual flow velocity of the sample in the measured suction nozzle is faster than a predetermined reference value, the basic aspiration time is corrected to be shortened, and the flow velocity is determined in advance. Characterized in that constitute the said control device to correct to extend late as basic suction time than Oita reference value.
Specifically, the basic aspiration time according to the standard viscosity specimen may be 2 seconds when a 100 μL specimen is aspirated through a flow path having an inner diameter of 0.7 mm.

A sample component analyzer according to the present invention includes a syringe mounting portion on which a syringe from which a sample is collected can be mounted, and a suction nozzle that enters the inside of the syringe mounted on the syringe mounting portion and sucks the sample inside the syringe A specimen component analyzer comprising: a suction pump connected to the suction nozzle; a sensor for analyzing a component of the specimen sucked by the suction nozzle; and a control device for controlling at least the operation of the suction pump; A means for measuring the flow rate of the sample in the suction nozzle is provided, and the basic suction time according to the standard viscosity sample is stored in the control device in advance, and the actual suction nozzle measured by the measurement means is measured. If the flow rate of the specimen is earlier than a predetermined reference value, the basic aspiration time is corrected to be shortened. If the flow rate is slower than the predetermined reference value, the basic flow rate is corrected. Since constitutes the said control device to correct to extend between the time of drawing, to lengthen the time for sucking the sample in the case of the flow rate is low analyte, in the case of the flow velocity is fast sample aspirating the specimen By shortening the time, the same amount of specimen can always be taken into the device. By configuring the suction time to be adjusted according to the difference in flow rate, that is, the difference in viscosity in this way, the suction speed can be increased, and as a result, the sample is taken into the apparatus. There is an effect that the time can be shortened.

1 is a perspective view of an embodiment of a sample component analyzer 1 according to the present invention. FIG. 3 is an enlarged view showing a state where a syringe S is attached to a syringe attachment portion in the sample component analyzer 1. It is a schematic block diagram which shows the structure of the sample suction mechanism of the sample component analyzer 1 which concerns on this invention. (A)-(c) is a figure which shows operation | movement of the suction nozzle 10 in the suction nozzle accommodating part 20. FIG.

  Embodiments of a sample component analyzer according to the present invention will be described below with reference to the accompanying drawings.

FIG. 1 is a perspective view of an embodiment of a sample component analyzing apparatus 1 according to the present invention, and FIG. 2 is an enlarged view showing a state where a syringe S is mounted on a syringe mounting portion of the sample component analyzing apparatus 1. Yes.
In the figure, reference numeral 2 denotes a casing of the sample component analyzer 1, reference numeral 3 denotes a monitor that also functions as an operation panel, and reference numeral 4 denotes a printer for printing the analysis result.
A calibration liquid tank 5, a cleaning liquid tank 7, and a waste liquid tank 6 are detachably mounted on the front surface of the casing 2.
A syringe mounting portion 8 for mounting the syringe S is provided in front of the upper surface of the casing 2. The syringe mounting part 8 is provided with a syringe detection sensor 8a that detects whether or not the syringe S is mounted (see FIG. 3).
Moreover, as shown in FIG.
A suction nozzle 10 configured to be able to be taken in and out of the syringe S attached to the attachment unit 8;
An operating part 11 for moving the suction nozzle 10;
A suction circuit 12 connected to the suction nozzle 10;
A pump 13 for generating a suction force on the suction nozzle 10 through the suction circuit 12;
A flow sensor 14 disposed upstream of the pump 13 in the suction circuit 12;
An analysis sensor unit 15 disposed between the pump 13 and the flow sensor 14 in the suction circuit 12;
And a control device 16 for controlling the above-described units.
Moreover, in FIG.1 and FIG.3, the code | symbol 20 has shown the suction nozzle accommodating part which accommodates the suction nozzle 10 so that the suction nozzle 10 can be operate | moved so that insertion / extraction is possible.
As shown in FIG. 2, the suction nozzle 10 has at least a tip portion 10a having a diameter capable of entering the inside of the syringe S and a body portion 10b connected to the tip portion 10a and having a diameter smaller than at least the tip portion 10a.
A first valve 21 and a second valve 22 that can be closed by the distal end portion 10 a of the suction nozzle 10 are arranged in the suction nozzle housing portion 20 at intervals along the moving direction of the suction nozzle 10. A cleaning liquid introduction port 23 and a waste liquid port 24 are provided between the two valves 21 and 22 in the suction nozzle accommodating unit 20. The specific operation will be described later, but if necessary, the cleaning liquid is supplied from the cleaning liquid tank 7 to the inside of the suction nozzle accommodating portion 20 from the cleaning liquid tank 7 through the cleaning liquid inlet 23 by a pump (or pump 13) (not shown). Then, the cleaning liquid is sucked into the suction nozzle through the pump 13, passes through the flow sensor 14 and the analysis sensor unit 15, and is discharged into the waste liquid tank 6. Further, the cleaning liquid that has not been sucked into the suction nozzle through the waste liquid port 24 is sucked into the waste liquid tank 6 by a pump (or pump 13) (not shown).
The control device 16 inputs signals from the syringe detection sensor 8a and the flow sensor 14, and controls the operations of the operation unit 11 and the pump 13 based on these signals.
Further, the control device 16 performs component analysis based on the detection signal from the analysis sensor unit 15 and outputs the analysis result to the monitor 3 and the printer 4. The component analysis process based on the detection signal from the analysis sensor unit 15 is the same process as that of a known component analysis apparatus, and thus detailed description thereof is omitted in this specification.

Next, the specimen aspirating function in the component analyzer 1 configured as described above will be described with reference to FIGS.
First, the user mounts the syringe S on the syringe mounting portion 8 (FIG. 4A).
The user presses the measurement button on the monitor 3.
When the syringe detection sensor 8a confirms that the syringe S is mounted, the control device 16 operates the operation unit 11 to advance the suction nozzle 10.
When the distal end portion 10a of the suction nozzle 10 enters the inside of the syringe S (FIG. 4B), the control device 16 operates the pump 13 to start aspiration of the sample in the syringe S with a predetermined suction force.
When a sample is detected by the flow sensor 14 after the start of aspiration, the control device 16 calculates a sample arrival time from the aspiration start time to the detection of the sample by the flow sensor 14, and based on the calculated sample arrival time in advance. The correction value for the basic suction time that has been determined is calculated. Then, the control device 16 controls the pump 13 based on the corrected suction time.
Specifically, if the actual specimen arrival time is shorter than the predetermined specimen arrival time, the basic aspiration time is corrected to be shortened, and conversely, the actual specimen arrival time is determined from the predetermined specimen arrival time. If it is longer, it is corrected to extend the basic suction time.
As a result, regardless of the viscosity of the sample, it is possible to always draw a certain amount of sample to the analysis sensor unit 15 with a predetermined fixed suction force (aspiration speed).
Further, as described above, by configuring the aspiration time to be corrected based on the specimen arrival time, the aspiration force can be determined according to a standard viscosity specimen. Specifically, in the conventional specimen component analyzer that does not control the suction time, the suction speed is set to match the specimen with high viscosity in order to draw a certain amount of specimen to the analysis sensor unit 15 regardless of the viscosity. Is set to a relatively slow speed. Specifically, when the sample is filled in the analysis sensor unit, 100 μL (depending on the number of measurement items), which is a sufficient amount of sample to measure, is about 7 seconds to aspirate with a flow path inner diameter of 0.7 mm. It is necessary to. If the flow path inner diameter is increased, the suction speed can be increased, but the amount of specimen increases and it becomes difficult to keep the suction amount constant. For this reason, even a specimen having a low viscosity is sucked slowly, so that the entire processing time becomes long.
On the other hand, the sample component analyzer of the present invention corrects the suction time based on the sample arrival time, so the suction speed can be adjusted to a standard viscosity sample. Is set to a faster speed. Specifically, when the sample is filled in the analytical sensor unit, 100 μL (which depends on the number of measurement items), which is a sufficient amount of sample to be measured, is aspirated at 0.7 mm in the inner diameter of the flow path. The sample can be aspirated at a rate of about 2 seconds. For this reason, the time required for aspirating the specimen is shortened as a whole, and the entire processing time can be shortened.
After the operation of the pump 13 is completed (that is, after the sample is aspirated), the control device 16 starts component analysis based on the detection result by the analysis sensor unit 15.
At the same time (or after the end of the analysis), the control device 16 moves the operating unit 11 to retract the suction nozzle 10 and pulls it out of the syringe S. The suction nozzle 10 has its tip 10a at the first valve 21 and the second valve. The valve 22 is retracted to a position where it is closed (FIG. 4C). As a result, a sealed cleaning space 20 a is formed between the first valve 21 and the second valve 22 in the suction nozzle housing portion 20.
After the analysis by the analysis sensor unit 15 is completed, the control device 16 operates a pump (or pump 13) (not shown) to supply the cleaning liquid from the cleaning liquid tank 7 into the cleaning space 20a via the cleaning liquid inlet 23.
After the cleaning liquid is supplied into the cleaning space 20 a, the control device 16 operates the pump 13 and sucks the cleaning liquid with the suction nozzle 10. The cleaning liquid sucked from the suction nozzle 10 is guided to the waste liquid tank 6 through the suction circuit 12. Thereby, the specimen in the suction nozzle 10 and the suction circuit 12 is washed with the washing liquid.
The control device 16 discharges all the specimen and cleaning liquid in the suction nozzle 10 and the suction circuit 12 to the waste liquid tank 6 and then operates a pump (or pump 13) (not shown) to leave the cleaning liquid remaining in the cleaning space 20a. Is sucked through the waste liquid port 24 and drained into the waste liquid tank 6.
When all the processes are completed, the process waits until the syringe S is next attached.

DESCRIPTION OF SYMBOLS 1 Specimen component analyzer 2 Casing 3 Monitor 4 Printer 5 Calibration liquid tank 6 Waste liquid tank 7 Washing liquid tank 8 Syringe mounting part 8a Syringe detection sensor 10 Suction nozzle 10a Tip part 10b Body part 11 Actuation part 12 Suction circuit 13 Pump 14 Flow sensor 15 Sensor unit for analysis 16 Control device 20 Suction nozzle storage unit 20a Cleaning space 21 First valve 22 Second valve 23 Cleaning liquid inlet 24 Waste liquid port

Claims (3)

A syringe mounting part capable of mounting a syringe from which a sample is collected;
An aspiration nozzle that enters the inside of the syringe attached to the syringe attachment portion and aspirates the specimen inside the syringe; and
A suction pump connected to the suction nozzle;
In a sample component analyzer comprising a sensor for analyzing a component of a sample sucked by the suction nozzle and a control device for controlling at least the operation of the suction pump,
Provide a means to measure the flow rate of the sample in the suction nozzle,
In the control device, the basic suction time according to the standard viscosity sample is stored in advance,
If the actual sample flow rate in the suction nozzle measured by the measuring means is earlier than a predetermined reference value, the basic aspiration time is corrected to be shortened, and the flow rate is delayed from the predetermined reference value. For example, the control device is configured to correct so as to extend the basic aspiration time . Basic aspiration time according to the standard viscosity sample,
2. The specimen component analyzer according to claim 1 , wherein the time is 100 seconds when a specimen of 100 μL is aspirated through a flow path having an inner diameter of 0.7 mm . The means for measuring the flow velocity is a flow sensor;
According to claim 1 or 2, characterized in that to calculate the flow velocity of the analyte in the control device on the basis of the time from the start of the operation of the suction pump until the flow sensor detects the analyte in the suction nozzle Sample component analyzer.

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