JP5571397B2 - Reagent preparation device - Google Patents

Description

  The present invention relates to a reagent preparation device capable of preparing a reagent by mixing liquids.

  Conventionally, a reagent preparation device capable of preparing a reagent by mixing a liquid is known (see, for example, Patent Document 1).

  Patent Document 1 discloses a reagent preparation apparatus that prepares a reagent by mixing a high concentration reagent and RO water. This reagent preparation device includes a reagent preparation tank for mixing RO water and a high concentration reagent. In the reagent preparation tank, there are provided a propeller for stirring the mixed liquid in which the RO water and the high concentration reagent are mixed, and a conductivity sensor for measuring the electric conductivity of the mixed liquid. The electrical conductivity measured by the conductivity sensor is used to determine whether or not the liquid mixture is diluted to a desired concentration.

WO2009 / 031461

  In the configuration where the conductivity sensor is provided in the reagent preparation tank as described above, if bubbles are generated on the liquid level in the reagent preparation tank, the bubbles adhere to the conductivity sensor as the water level rises or falls. Resulting in. In the reagent preparation apparatus of Patent Document 1, a high concentration reagent and RO water are supplied to the reagent preparation tank at a low flow rate in order to suppress the generation of bubbles, and the mixed solution is supplied by a propeller provided in the reagent preparation tank. It was stirring.

The present invention has been made to solve the above-described problems, and one object of the present invention is to provide a reagent preparation apparatus that can obtain accurate electrical conductivity with a simpler configuration than the conventional one. is there.

Means for Solving the Problems and Effects of the Invention

In order to achieve the above object, a reagent preparation device according to one aspect of the present invention supplies a mixed liquid containing a first liquid and a second liquid as a reagent to a measurement unit that measures a specimen using a reagent. A reagent preparation device configured to be capable of mixing a first liquid and a second liquid, provided downstream from the mixing container, and supplying the mixed liquid supplied from the mixing container to the inside An electric conductivity acquisition unit that acquires the electric conductivity of the mixed liquid that passes through the reagent flow path and passes through the reagent flow path, and a liquid temperature acquisition that is provided downstream of the mixing container and acquires the liquid temperature of the mixed liquid And the temperature of at least one of the first liquid, the second liquid, and the liquid mixture is different from the ambient temperature. to, adjusting the temperature of the liquid as close to ambient temperature By that, that suppresses the temperature adjusting unit the temperature gradient of the mixed solution through the electrical conductivity acquiring unit and the liquid temperature acquiring unit the liquid temperature of the mixture close to the ambient temperature, obtained by electrical conductivity acquiring unit electric A controller that controls the flow of the mixed liquid based on the conductivity and the liquid temperature acquired by the liquid temperature acquisition unit;

  As described above, the reagent preparation device according to one aspect of the present invention includes the electrical conductivity acquisition unit that allows the liquid mixture supplied from the mixing container to pass therethrough and acquires the electrical conductivity of the liquid mixture that passes therethrough. This prevents the liquid level in the mixing container from coming into contact with the electrical conductivity acquisition unit, thereby suppressing the influence on the electrical conductivity acquisition unit when bubbles are generated on the liquid level in the mixing container. be able to. Therefore, for example, without providing a propeller in the tank, the flow rate when supplying the first liquid and the second liquid to the mixing container may be increased, whereby the mixed liquid may be stirred. The configuration can be simplified. Furthermore, the reagent preparation device according to this aspect includes a liquid temperature acquisition unit that acquires the liquid temperature of the mixed liquid, and temperature adjustment that adjusts the temperature of the liquid upstream of the liquid temperature acquisition unit and the electrical conductivity acquisition unit. A part. Thereby, the temperature-adjusted liquid mixture can be supplied to the electrical conductivity acquisition unit and the liquid temperature acquisition unit. Thereby, the temperature difference between the liquid temperature of the mixed liquid when the electric conductivity is measured by the electric conductivity acquisition unit and the liquid temperature acquired by the liquid temperature acquisition unit can be reduced, and accurate electric conduction Based on the degree, the flow of the mixture can be controlled.

In the reagent preparing device according to the above aspect, the temperature adjustment unit preferably includes a heat-conductive tube member, and allows the liquid to pass through the tube member to exchange heat between the liquid and the outside of the tube member. Accordingly , the temperature of the liquid passing through the tube member is configured to be close to the ambient temperature around the temperature adjustment unit . If comprised in this way, since the liquid supplied can be passed through the inside of the pipe member which has heat conductivity, heat exchange with a liquid and the exterior (atmosphere) can be performed, Therefore Configurations, such as a temperature sensor and a heater Without using the liquid temperature, the liquid temperature can be easily adjusted with a simple configuration.

  In this case, the tube member is preferably configured so that the liquid passes while changing its direction. According to such a structure, the area | region which a pipe member occupies can be made small, lengthening the distance which a liquid passes a pipe member. Thereby, the temperature of a liquid can be adjusted efficiently, without enlarging an apparatus.

  In the configuration in which the temperature adjusting unit includes a pipe member having thermal conductivity, the pipe member having thermal conductivity is preferably made of metal. By configuring the tube member with such a metal having high thermal conductivity and easy processing, a highly thermally conductive tube member capable of effectively exchanging heat between the liquid and the outside (atmosphere) Can be easily obtained.

  In the reagent preparing device according to the above aspect, the temperature adjusting unit may be provided upstream of the mixing container and configured to adjust the temperature of the first liquid.

  In this case, preferably, the first liquid is pure water. According to such a configuration, it is not necessary to clean the temperature control unit after supplying the liquid to the temperature control unit.

  In the configuration in which the temperature adjustment unit adjusts the temperature of the first liquid, preferably, the temperature adjustment unit further includes a quantitative supply unit that quantifies the first liquid and the second liquid and supplies them to the mixing container. A larger amount of the first liquid than the second liquid is supplied to the mixing container. If comprised in this way, since the temperature of a liquid mixture can be adjusted only by adjusting the temperature of a 1st liquid, it is not necessary to provide the temperature adjustment part for adjusting the temperature of a 2nd liquid, The configuration can be simplified.

  The reagent preparation device according to the above aspect preferably further includes a disposal port for discarding the mixed solution, and the control unit acquires the electrical conductivity acquired by the electrical conductivity acquisition unit and the liquid temperature acquisition unit. Based on the measured liquid temperature, it is determined whether or not the liquid mixture satisfies a predetermined condition, and when the predetermined condition is not satisfied, the flow of the liquid mixture is controlled so that the liquid mixture flows to the disposal port. It is configured. If comprised in this way, the quality (concentration) of the liquid mixture used as a reagent will be judged based on the electrical conductivity of a liquid mixture, and the low quality liquid mixture which does not satisfy | fill predetermined conditions can be discarded from a disposal port. Therefore, it is possible to supply only a reagent (mixed solution) having an appropriate concentration with no quality problem to the measurement unit.

  In the reagent preparing device according to the above aspect, the liquid temperature acquisition unit is preferably provided inside the electrical conductivity acquisition unit. If comprised in this way, the deviation of the liquid temperature when electrical conductivity is acquired by the electrical conductivity acquisition part and the liquid temperature obtained by the liquid temperature acquisition part will become smaller.

In this case, the reagent flow path, have a Tokoro constant length of, the liquid temperature acquisition unit may be provided at a position deviated from the center position of the reagent flow channel of the electrical conductivity acquiring unit.

  The reagent preparation device according to the above aspect preferably further includes a stirring unit that stirs the mixed solution by flowing into the container. If comprised in this way, since a liquid mixture can be easily stirred by flowing a liquid mixture into a container, a liquid mixture can be stirred, for example, without providing a propeller, and a structure can be simplified. it can.

It is the perspective view which showed the blood analyzer provided with the reagent preparation apparatus by one Embodiment of this invention. It is the schematic diagram which showed the structure of the reagent preparation apparatus by one Embodiment shown in FIG. It is sectional drawing which showed the heat exchanger of the reagent preparation apparatus by one Embodiment shown in FIG. It is sectional drawing which showed the electrical conductivity measurement unit for measuring the electrical conductivity of the reagent of the reagent preparation apparatus by one Embodiment shown in FIG. It is a schematic diagram for demonstrating the measurement of the electrical conductivity of the reagent by the electrical conductivity measurement unit shown in FIG. It is a block diagram for demonstrating the control part of the reagent preparation apparatus by one Embodiment of this invention. It is a flowchart for demonstrating the reagent preparation process operation | movement of the reagent preparation apparatus by one Embodiment of this invention.

  Hereinafter, embodiments of the present invention will be described with reference to the drawings.

  First, with reference to FIGS. 1-6, the structure of the blood analyzer 1 by one Embodiment of this invention is demonstrated. Further, in the present embodiment, a case will be described in which the reagent preparing device 4 according to an embodiment of the present invention is used as a part of the blood analyzer 1 for performing a blood test.

  As shown in FIG. 1, the blood analyzer 1 includes a measurement unit 2 having a function of measuring blood, a data processing unit 3 that analyzes measurement data output from the measurement unit 2 and obtains an analysis result, and a sample. And a reagent preparation device 4 that prepares a reagent used for the above process. The measurement unit 2 is configured to measure white blood cells, reticulocytes, and platelets in blood by flow cytometry. The measuring unit 2 is configured to measure white blood cells, reticulocytes, and platelets by diluting blood using the reagent prepared and supplied by the reagent preparing device 4. The flow cytometry method forms a sample flow including a measurement sample and irradiates the sample flow with a laser beam, whereby forward scattered light and side scattered light emitted from particles (blood cells) in the measurement sample. And a method for measuring particles (blood cells) for detecting side fluorescence.

  In addition, a pneumatic unit 8 installed outside the housing is connected to the measuring unit 2, and various liquids are transferred in the apparatus using the negative pressure and the positive pressure supplied from the pneumatic unit 8. It is configured as follows. The pneumatic unit 8 has a negative pressure source 81 for supplying a negative pressure to the measuring unit 2 and a positive pressure source 82 for supplying a positive pressure. By using the negative pressure of the negative pressure source 81, the reagent used for the measurement is sucked from the reagent preparing device 4 to the measuring unit 2 (the reagent is supplied from the reagent preparing device 4).

  The data processing unit 3 includes a personal computer (PC) and the like, and has a function of analyzing the measurement data of the measurement unit 2 and displaying the analysis result. The data processing unit 3 includes a control unit (PC main body) 31, a display unit 32, and an input device 33.

  The control unit 31 is communicably connected to the measurement unit 2 and the reagent preparation device 4 via a communication interface (not shown). In addition to receiving measurement data of the measurement unit 2, the control unit 31 receives a measurement start signal and a shutdown signal. And a function of transmitting to the reagent preparing device 4. The user can use the input device 33 to select a measurement mode, start up and shut down the measurement unit 2 and the reagent preparation device 4.

  The display unit 32 has a function of displaying an image (screen) in accordance with the video signal input from the control unit 31.

  Here, in this embodiment, the reagent preparation device 4 is provided for preparing a reagent used in the measurement unit 2. Specifically, the reagent preparation device 4 dilutes the high concentration reagent (reagent stock solution) to a desired concentration using RO water prepared from tap water by an RO water preparation unit (RO water supply unit) 7 described later. Thus, a reagent used for blood analysis is prepared. The RO water is a kind of pure water, and is water from which impurities are removed by permeating through an RO (Reverse Osmosis) membrane (reverse osmosis membrane). Pure water is purified water, deionized water, distilled water, and the like in addition to RO water, and has been subjected to treatment for removing impurities, but its purity (water quality) is not particularly limited.

  As shown in FIG. 2, the reagent preparation device 4 includes a high concentration reagent chamber 41, an RO water chamber 42, a first dilution chamber 43, a second dilution chamber 44, and two diaphragm pumps 45a provided in the housing. 45b, a stirring chamber 46, a supply chamber 47, and a control unit 48 for controlling the operation of each part of the reagent preparing device 4. Furthermore, as shown in FIG. 1, the reagent preparation device 4 is connected to a high concentration reagent tank 5, an air pressure unit 6, and an RO water preparation unit (RO water supply unit) 7 installed outside the casing. ing. The reagent preparation device 4 obtains the high concentration reagent and the RO water from the high concentration reagent tank 5 and the RO water preparation unit 7, respectively, and uses the negative pressure and the positive pressure supplied from the pneumatic pressure unit 6 in the device. Each liquid is transferred. The pneumatic unit 6 has a negative pressure source 61 for supplying a negative pressure to the reagent preparing device 4 and a positive pressure source 62 for supplying a positive pressure. In the following description, the direction in which the liquid is directed toward the measurement unit 2 is the downstream side, and the RO water preparation unit 7 (or the high concentration reagent tank 5) side into which the liquid (RO water or the like) flows into the reagent preparation device 4 is the upstream side. To do.

  As shown in FIG. 2, the high concentration reagent chamber 41 is configured to be supplied with a high concentration reagent from the high concentration reagent tank 5. The high concentration reagent chamber 41 is provided with a float switch 100 for detecting that a predetermined amount of high concentration reagent is accommodated in the chamber. The float switch 100 is configured such that the float part moves up and down in accordance with the amount of liquid (liquid level) in the high concentration reagent chamber 41. When the float part of the float switch 100 reaches the lower limit, the float part reaches the upper limit. Each part is controlled by the control unit 48 so that the high concentration reagent is supplied from the high concentration reagent tank 5 to the high concentration reagent chamber 41. Thus, the high concentration reagent chamber 41 is always supplied with the high concentration reagent so that about 300 mL is stored.

  The high-concentration reagent chamber 41 is connected to the high-concentration reagent tank 5 through an electromagnetic valve 200 and is connected to a negative pressure source 61 of the pneumatic unit 6 through an electromagnetic valve 201. The high concentration reagent chamber 41 is configured to be opened to the atmosphere or closed by opening / closing the electromagnetic valve 202. The high concentration reagent chamber 41 is connected to a flow path 301 for transferring a liquid from the diaphragm pump 45a (45b) to the first dilution chamber 43 (second dilution chamber 44) by a flow path 300. In addition, an electromagnetic valve 203 is provided on the flow path 300, and the flow of the high concentration reagent into the flow path 301 is controlled by opening and closing the electromagnetic valve 203.

  The RO water chamber 42 is configured such that RO water for diluting the high concentration reagent is supplied from the RO water preparation unit 7. The RO water chamber 42 is provided with float switches 101 and 102 for detecting that the upper limit amount and the lower limit amount of RO water accommodated in the chamber has been reached. The float switch 101 (102) is configured such that the float part moves up and down according to the amount of liquid (liquid level) in the RO water chamber. When the float part of the float switch 101 reaches a position corresponding to the upper limit amount (about 600 mL) of the RO water chamber 42, the supply of the RO water from the RO water preparation part 7 to the RO water chamber 42 is stopped, and the float switch 102 When the float unit reaches a position corresponding to the lower limit amount (about 300 mL) of the RO water chamber 42, each unit is controlled by the control unit 48 so that RO water is supplied from the RO water preparation unit 7 to the RO water chamber 42. It is comprised so that. Thereby, while the reagent preparation device 4 is operating, the RO water chamber 42 stores about 300 mL or more and about 600 mL or less of RO water.

  Here, the RO water preparation unit 7 and the reagent preparation device 4 are connected via an intake port 500. The RO water (pure water) supplied from the RO water preparation unit 7 is taken into the reagent preparation device 4 from the intake port 500, passes through the flow path 501, and is supplied to the RO water chamber 42. ing. In addition, an electromagnetic valve 207 is provided in the flow path 501, and the inflow (supply) of RO water to the flow path 501 in the reagent preparing device 4 is controlled by opening and closing the electromagnetic valve 207. .

  The RO water chamber 42 is configured to be able to discard the RO water in the chamber. Specifically, the RO water chamber 42 is connected to the positive pressure source 62 via the electromagnetic valve 204 and is connected to the waste flow path via the electromagnetic valve 205, and both the electromagnetic valves 204 and 205 are connected. By opening the, the internal RO water is pushed out to the waste channel with positive pressure. The RO water chamber 42 is configured to be opened to the atmosphere or closed by opening / closing the electromagnetic valve 206. The RO water chamber 42 is connected to the diaphragm pumps 45a and 45b by the flow path 302 via the electromagnetic valve 208.

  Here, in the present embodiment, the temperature of the liquid supplied to the flow path 302 from the RO water chamber 42 to the diaphragm pumps 45a and 45b on the downstream side of the intake port 500 is set to the ambient temperature (inside the casing). In addition, a heat exchanger 400 for adjusting the temperature so as to be close to the ambient temperature of the heat exchanger 400 is provided. As described above, since RO water is produced using tap water, the temperature of the RO water supplied from the RO water production unit 7 and the blood analyzer 1 that is installed indoors and used in a state close to room temperature ( The temperature of the reagent preparation device 4) and the high concentration reagent may be greatly different. The heat exchanger 400 has a function of adjusting the temperature of the RO water to be close to the ambient temperature by exchanging the supplied RO water with the outside air of the heat exchanger 400.

  As shown in FIG. 3, the heat exchanger 400 includes three pipe members 401, 402, and 403 having thermal conductivity and connection members 404, 405, 406 for connecting the pipe members 401 to 403, respectively. 407, and the liquid (RO water) is allowed to pass through each of the pipe members 401 to 403 so that heat exchange between the RO water and the outside can be performed.

  The tube members 401, 402, and 403 are made of metal (for example, SUS316L) and have the same shape. Specifically, each of the tube members 401, 402, and 403 has a cylindrical shape having a length L (for example, about 300 mm), and connecting portions 401a, 402a, and 403a are provided at both ends, respectively. The tube members 401, 402, and 403 have an outer diameter d1 (for example, about 8 mm) and an inner diameter d2 (for example, about 3 mm), respectively. When the RO water passes through the flow path having the inner diameter d2, heat exchange between the RO water and the outside is performed, so that the temperature of the RO water is adjusted to approach the ambient temperature. More specifically, when the supplied RO water is lower than the ambient temperature, the heat exchanger 400 increases the temperature of the RO water by exchanging the supplied RO water with the outside air of the heat exchanger 400. Let Further, when the supplied RO water is higher than the ambient temperature, the heat exchanger 400 lowers the temperature of the RO water by exchanging the supplied RO water with the outside air of the heat exchanger 400.

  Each of the connection members 404 to 407 is made of a bendable tubular member such as a urethane tube. The connecting member 404 is provided in the connecting portion 401a on one side (upstream side) of the pipe member 401, and is connected to the RO water chamber 42 side. The connecting member 405 connects the other side (downstream side) connecting part 401 a of the pipe member 401 and the one side (upstream side) connecting part 402 a of the pipe member 402. The connecting member 406 connects the other side (downstream side) connecting part 402 a of the tube member 402 and the one side (upstream side) connecting part 403 a of the tube member 403. The connecting member 407 is provided at the connecting portion 403a on the other side (downstream side) of the pipe member 403, and is connected to the diaphragm pumps 45a and 45b side. As a result, the RO water supplied from the RO water chamber 42 and flowing in from the connection member 404 passes through the three pipe members 401, 402, and 403 while changing the direction while exchanging heat with the outside, and the connection member 407. To flow out to the diaphragm pumps 45a and 45b side. As described above, in this embodiment, the RO water passes through the three tube members 401, 402, and 403 while changing the direction, so that the space occupied by the heat exchanger 400 in the reagent preparation device 4 is reduced. However, the distance that the RO water passes through the pipe members 401 to 403 can be increased. Therefore, the RO water can be efficiently heat-exchanged without increasing the size of the reagent preparation device 4.

  As shown in FIG. 2, the first dilution chamber 43 and the second dilution chamber 44 are provided for diluting the high concentration reagent with RO water, respectively. Further, as will be described later, the first dilution chamber 43 (second dilution chamber 44) is configured to be able to accommodate about 300 mL of liquid (mixed liquid of high concentration reagent and RO water) fed by the diaphragm pumps 45a and 45b. ing. The first dilution chamber 43 (second dilution chamber 44) is moved up and down to detect that the remaining amount of liquid (mixed liquid of high concentration reagent and RO water) contained in the chamber has reached a predetermined amount. A possible float switch 103 (104) is provided. The first dilution chamber 43 (second dilution chamber 44) is configured to be always open to the atmosphere. The first dilution chamber 43 (second dilution chamber 44) is connected to the flow path 301 by a flow path 303 (304) through an electromagnetic valve 209 (210). By controlling the opening and closing of the electromagnetic valves 209 and 210, the liquid (RO water and high concentration reagent) transferred through the flow path 301 is supplied from the flow path 303 to the first dilution chamber 43, or the flow path It is possible to select whether to supply the second dilution chamber 44 from 304. The first dilution chamber 43 (second dilution chamber 44) is connected to the agitation chamber 46 through an electromagnetic valve 211 (212).

  The diaphragm pumps 45a and 45b have the same configuration as each other and are configured to perform the same operation at the same time. The diaphragm pump 45a (45b) has a function of quantifying the high concentration reagent and the RO water by about 6.0 mL (fixed amount) in one quantification operation, and has a total of about 12 mL (about 6. It is configured to supply 0 mL × 2) of liquid. The diaphragm pump 45a (45b) is connected to the negative pressure source 61 via the electromagnetic valve 213 (215), and is connected to the positive pressure source 62 via the electromagnetic valve 214 (216).

  The supply operation of the liquid (RO water and high concentration reagent) by the diaphragm pumps 45a and 45b includes the inflow of the liquid by the negative pressure source 61 through the electromagnetic valve 213 (215) and the positive pressure through the electromagnetic valve 214 (216). It includes two processes: liquid outflow from the source 62. In each process, the flow path is selected from the flow paths 300 to 304 in accordance with the opening / closing control of the electromagnetic valves 203, 208, 209, 210, etc., so that a high concentration is obtained from the high concentration reagent chamber 41 or the RO water chamber 42. A reagent or RO water is introduced and supplied to the first dilution chamber 43 or the second dilution chamber 44. In this embodiment, the diaphragm pumps 45a and 45b are configured to supply a larger amount of RO water than the high concentration reagent. Specifically, about 12.0 mL × 24 times = about 288 mL of RO water is sucked from the RO water chamber 42 by the diaphragm pumps 45 a and 45 b and supplied to the first dilution chamber 43 or the second dilution chamber 44. On the other hand, about 12.0 mL × one time = about 12 mL of high concentration reagent is sucked from the high concentration reagent chamber 41 and supplied to the first dilution chamber 43 or the second dilution chamber 44. Therefore, about 288 mL (RO water) + about 12 mL (high concentration reagent) = about 300 mL of a mixed solution is supplied.

  The agitation chamber 46 is configured to be capable of accommodating about 300 mL of liquid, and agitates the liquid (mixed liquid of high concentration reagent and RO water) supplied from the first dilution chamber 43 (second dilution chamber 44). Is provided. Specifically, the stirring chamber 46 has a bent pipe 461. The pipe 461 has a convection by flowing a liquid (mixed liquid of high concentration reagent and RO water) supplied from the first dilution chamber 43 (second dilution chamber 44) along the inner wall surface of the stirring chamber 46. The high concentration reagent and the RO water are generated and agitated. Therefore, according to the present embodiment, it is possible to stir the high concentration reagent and the RO water with a simple configuration without providing a mechanism for stirring the liquid mixture like a propeller.

  The stirring chamber 46 is provided with a float switch 105 that can move up and down to detect that the remaining amount of the liquid (mixed liquid of high concentration reagent and RO water) stored in the chamber has reached a predetermined amount. Yes. When the float part of the float switch 105 reaches the lower limit and the chamber becomes empty, the electromagnetic valve 211 (or 212) and the electromagnetic valve 217 are opened, and the electromagnetic valve 212 (or 211) and the electromagnetic valve 218 are closed. Accordingly, the control unit is configured so that approximately 300 mL of the mixed liquid (the total amount of the mixed liquid contained in the chamber) is supplied from one of the first dilution chamber 43 and the second dilution chamber 44 to the stirring chamber 46. Each part is controlled by 48.

  The supply chamber 47 is provided for storing and storing a reagent that waits for supply to the measurement unit 2. The supply chamber 47 can store a reagent (stirred mixed liquid) having a maximum liquid volume of about 600 mL. The supply chamber 47 includes a float switch 106 for detecting that the remaining amount of the reagent accommodated in the chamber has reached about 300 mL, and a float for detecting that the remaining amount of the reagent is substantially zero. A switch 107 is provided. The float switch 106 (107) is configured such that the float part moves up and down in accordance with the amount of liquid (liquid level) in the supply chamber 47. When the float part of the float switch 106 reaches the lower limit position, each part is controlled by the control part 48 so that about 300 mL of reagent with a desired concentration is supplied from the stirring chamber 46 to the supply chamber 47. .

  Further, when the float switch 107 detects that the remaining amount of the reagent stored in the chamber has become substantially zero, the supply of the reagent to the measurement unit 2 is stopped. Thereby, even if the reagent is not supplied to the supply chamber 47 for some reason, it is possible to prevent bubbles from being mixed into the reagent supplied to the measurement unit 2 while continuing the supply of the reagent to the measurement unit 2 as much as possible. Is possible.

  The supply chamber 47 is connected to the stirring chamber 46 through an electromagnetic valve 219. Further, the supply chamber 47 is configured such that the reagent in the chamber can be discarded during maintenance or the like by opening the electromagnetic valve 220. Further, the supply chamber 47 is configured so as to be always open to the atmosphere. The supply chamber 47 is connected to the measurement unit 2 via a filter 471. The filter 471 is provided to prevent impurities from entering the reagent supplied to the measurement unit 2.

  In the present embodiment, an electrical conductivity measurement unit 410 is provided in the flow path between the stirring chamber 46 and the supply chamber 47. The electrical conductivity measurement unit 410 includes an electrical conductivity meter and a temperature sensor (thermistor), and has a function of measuring the electrical conductivity of the reagent and the temperature of the reagent. Since the reagent concentration and electrical conductivity have a predetermined relationship, the concentration of the prepared reagent is appropriate by measuring the electrical conductivity of the reagent (mixed liquid) in which RO water and high-concentration reagent are mixed. It is possible to determine whether the density is high. In addition, a waste flow path that reaches the waste port 502 via the electromagnetic valve 221 is connected between the electrical conductivity measurement unit 410 and the electromagnetic valve 219. When the measured concentration of the reagent is not a desired concentration, the reagent is supplied to the disposal channel and discarded from the disposal port 502.

  As shown in FIG. 4, the electrical conductivity measurement unit 410 includes a first main body 411 and a second main body 412, and a third main body having an outlet 413 a through which the reagent that has passed therethrough flows out to the supply chamber 47 side. 413, an electrode 414 having a reagent inflow port 414a from the stirring chamber 46 side, an electrode 415 provided between the first body portion 411 and the second body portion 412, and the second body portion 412 and the third body. The electrode 416 provided between the parts 413 and the thermistor 417 for measuring the temperature of a reagent are included. Each of the first main body portion 411, the second main body portion 412, the third main body portion 413, the electrode 414, the electrode 415, and the electrode 416 has a reagent channel 418 formed therein, and an inlet 414a of the electrode 414 is formed. The reagent that has flowed in from the inside passes through the inside and flows out from the outflow port 413a of the third main body 413. The reagent flow path 418 formed inside the electric conductivity measuring unit 410 is provided so that the inner diameters d3 are substantially equal, and it is possible to suppress the generation of bubbles that are likely to occur at the joint portion when the reagent passes. It is configured as follows. With such a configuration, the electrical conductivity measurement unit 410 is configured to measure the electrical conductivity of the reagent passing between the electrode 414, the electrode 415, and the electrode 416.

Specifically, as shown in FIG. 5, the electrodes 414 and 416 at both ends are grounded, and the center electrode 415 is connected to an AC power source, and between the electrodes 414 and 415 and between the electrodes 415 and 416. Apply voltage. At this time, the resistance value R1 of the reagent existing in the first measurement region 410a between the electrodes 414 and 415 and the resistance value R2 of the reagent existing in the second measurement region 410b between the electrodes 415 and 416 are detected, respectively. , electrical conductance Z _T of the reagent (mixed solution) is measured based on the combined resistance R of the resistance values R1 and R2. By connecting the central electrode 415 to the AC power source and grounding the electrodes 414 and 416 at both ends in this way, electricity is leaked to the outside of the electrical conductivity measurement unit 410 (electrodes 414, 415 and 416). And it is comprised so that it can suppress that measurement accuracy falls.

Further, since the measured electrical conductivity Z _T varies depending on the temperature, temperature compensation is performed using the reagent temperature T (° C.) measured by the thermistor 417, and the control unit 48 converts the temperature to a reference temperature (25 ° C.). The electric conductivity Z _r (25 ° C. converted value) of the obtained reagent is calculated.
The electrical conductivity Z _r (25 ° C. converted value) is calculated based on, for example, the following equation using the temperature change rate A with respect to the electrical conductivity of the reagent.
Z _r = Z _T × {1 + A × (T−25)}
Although the temperature change rate A varies depending on the type and concentration of the liquid, for example, 0.02 is used.
_Then, the Z r (25 ° C. conversion value), the target value of the reagent concentration in the previously obtained reference temperature by experiment (25 ° C.) by comparing the (proper value) _{Z 0,} the target value _{Z 0} is _{Z r} On the other hand, the controller 48 determines whether or not the reagent concentration is appropriate (whether or not the reagent is to be discarded from the disposal port 502) by determining whether or not it is within a predetermined range. ing.

  As shown in FIG. 4, in the present embodiment, the thermistor 417 is provided in the third main body 413 and is provided so as to extend in the reagent channel 418 to substantially the same position as the electrode 416. Accordingly, the thermistor 417 is disposed on the second measurement region 410b side of the electrical conductivity measurement unit 410 and is configured to measure the temperature of the reagent in the vicinity of the electrode 416.

Here, when the temperature of the reagent and the ambient temperature (the ambient temperature outside the electrical conductivity measurement unit 410) are different from each other, the reagent is heat-exchanged by the electrical conductivity measurement unit 410 so as to approach the ambient temperature. While passing through the electrical conductivity measuring unit 410. Therefore, the temperature difference between the liquid temperature of the reagent and the ambient temperature is large at the inlet of the reagent channel 418 and small at the outlet, and a temperature gradient of the reagent is generated inside the electrical conductivity measurement unit 410 (reagent channel 418). Will do. Therefore, as shown in FIG. 5, the reagent temperature in the vicinity of the electrode 414 immediately after flowing into the electric conductivity measuring unit 410 is T1 (° C.), the temperature in the vicinity of the electrode 415 is T2 (° C.), and the electric conductivity measuring unit 410 It is assumed that the temperature in the vicinity of the electrode 416 that is near the outlet of the gas reaches T3 (° C.) (T1 <T2 <T3, where the ambient temperature is higher than the reagent temperature). In this case, the resistance value R1 of the reagent measured in the first measurement region 410a is a value reflecting the resistance value (electrical conductivity) of the reagent at a temperature between temperatures T1 (° C.) and T2 (° C.). . On the other hand, the resistance value R2 of the reagent measured in the second measurement region 410b is a value reflecting the resistance value (electric conductivity) of the reagent at a temperature between temperatures T2 (° C.) and T3 (° C.). As a result, a value reflecting the temperature T2 (° C.) is generally obtained as the combined resistance R, and therefore the electric conductivity Z _T (corresponding to T2 ° C.) corresponding to the electric conductivity of the reagent at the temperature T2 (° C.) is measured. The However, at this time, the temperature measured by the thermistor 417 on the second measurement region 410b side is T3 (° C.).

In this case, the measured electrical conductivity Z _T (corresponding to T2) is converted into the electrical conductivity Z _r (25 ° C. converted value) using the reagent temperature T3 (° C.) measured by the thermistor 417. End up. As a result, an accurate electric conductivity Z _r (25 ° C. converted value) cannot be obtained.

Furthermore, the reagent to be measured for going through the reagent flow path 418 of the electrical conductance measuring unit 410, a position constantly change of the reagent even while performing the measurement of the electrical conductivity Z _T and a temperature T To do. Measurements of the electrical conductivity Z _T, while capable of acquiring immediately by applying a voltage between the electrodes 414 to 416, measurement of the temperature T by the thermistor 417, than the measurement time of the electrical conductance Z _T Long measurement time is required. Therefore, for example, even if the thermistor 417 attempts to measure the temperature T2 in the vicinity of the electrode 415, the temperature measurement result is obtained after the reagent has passed through the thermistor 417. Therefore, the measured electric conductivity Z _T is equal to the temperature T2. May be the electrical conductivity of the reagent flowing in a different position from the obtained reagent. If the temperature gradient is generated in this way reagent temperature due to the deviation between the temperature and the ambient temperature of the reagent, it is difficult to accurately measure the temperature T of the electrical conductance Z _T measurement time point of the reagent .

Therefore, in this embodiment, the temperature of the supplied RO water is adjusted by the heat exchanger 400 so as to approach the ambient temperature, whereby the reagent (mixed liquid) in which the RO water and the high concentration reagent are mixed is changed to the ambient temperature. Since it can be supplied to the electrical conductivity measuring unit 410 at a temperature close to, it is possible to suppress the temperature gradient of the reagent by suppressing the temperature change of the reagent in the electrical conductivity measuring unit 410. That is, the temperature of the reagent at each position in the electrical conductivity measurement unit 410 (reagent channel 418) can be set to T1 (° C.) ≈T2 (° C.) ≈T3 (° C.) (≈T ° C.). As a result, the temperature (T ° C.) the measured electrical conductance Z _T reflects, since the temperature difference in the temperature (T ° C.) of the measured reagent by the thermistor 417 is prevented from occurring, measured It is possible to obtain an accurate electrical conductivity Z _r (25 ° C. converted value) based on the electrical conductivity Z _T and the temperature (T ° C.) of the reagent.

  In addition, as shown in FIG. 2, the RO water preparation unit (RO water supply unit) 7 connected to the reagent preparation device 4 uses RO water as a dilution liquid for diluting a high concentration reagent, and tap water. It is comprised so that it can produce using. The RO water production unit 7 includes an RO water storage tank 7a, an RO membrane 7b, and a filter 7c for protecting the RO membrane 7b by removing impurities contained in tap water. Furthermore, the RO water preparation unit 7 includes a high-pressure pump 7d that applies high pressure to the water that has passed through the filter 7c so that water molecules pass through the RO membrane 7b, and an electromagnetic valve 7f that controls the supply of tap water. .

  The RO water storage tank 7a is provided to store the RO water that has passed through the RO membrane 7b. The RO water storage tank 7a is provided with a float switch 7e for detecting that a predetermined amount of RO water is stored, and an electrical conductivity measurement unit 7g for detecting the quality of the RO water. . The rate at which RO water is supplied from the RO water preparation unit 7 to the RO water storage tank 7a, that is, the RO water production rate by the RO water production unit 7 is about 20 L / hour or more and about 50 L / hour or less.

  As shown in FIG. 6, the control unit 48 is connected to each part in the reagent preparing device 4 via a CPU 48 a, a ROM 48 b, a RAM 48 c, a communication interface 48 d connected to the data processing unit 3, and each circuit. I / O (Input / Output) section 48e.

  The CPU 48a is provided for executing computer programs stored in the ROM 48b and computer programs loaded in the RAM 48c. The CPU 48a is configured to use the RAM 48c as a work area when executing these computer programs.

  The communication interface 48d is configured to be able to transmit various types of information to the data processing unit 3 so that the user can confirm errors that have occurred in the reagent preparation device 4.

  As shown in FIG. 6, the I / O unit 48 e is configured such that signals are input from the float switches 100 to 107 and the electrical conductivity measurement unit 410 via each sensor circuit. Further, the I / O unit 48e is configured to output a signal to each driving circuit in order to control driving of the electromagnetic valves 200 to 221 and the pneumatic unit 6 and the like via each driving circuit.

  Next, the reagent preparation processing operation of the reagent preparing device 4 according to the embodiment of the present invention will be described with reference to FIGS.

  As shown in FIG. 2, the reagent preparation processing operation is started when the user instructs the apparatus activation from the data processing unit 3, that is, when the reagent preparation device 4 receives the activation signal from the data processing unit 3. When the reagent preparation processing operation is started, first, in step S1 of FIG. 7, the CPU 48a initializes the computer program stored in the ROM 48b.

  In step S2, the RO water preparation unit 7 starts the RO water preparation process. That is, the high-pressure pump 7d is driven, and the water that has passed through the filter 7c passes through the RO membrane 7b due to the high pressure. Then, based on the detection result of the float switch 7e, the RO water is supplied to the RO water storage tank 7a until a predetermined amount of RO water is stored in the RO water storage tank 7a.

  Next, in step S <b> 3, the electromagnetic valve 207 is opened by the CPU 48 a, and RO water is supplied to the RO water chamber 42 via the intake port 500. At this time, a predetermined amount of RO water is supplied to the RO water chamber 42 based on the detection result of the float switch 101 (102). Note that the RO water supply process is continued after step S3, and RO water is sequentially supplied to the RO water chamber 42 based on the detection result of the float switch 101 (102).

  In step S4, the CPU 48a determines whether or not a predetermined amount of reagent is stored in the supply chamber 47. That is, based on the detection result of the float switch 106, it is determined whether or not a predetermined amount (about 300 mL or more and about 600 mL or less) of the reagent is stored in the supply chamber 47. If a predetermined amount of reagent is stored, the process proceeds to step S12.

  On the other hand, if a predetermined amount of reagent is not stored, supply of the high concentration reagent and RO water to the first dilution chamber 43 (or the second dilution chamber 44) by the diaphragm pumps 45a and 45b in step S5. Is done. Specifically, the valve 208 is opened, and the valve 213 (215) is opened. Due to the negative pressure applied by the negative pressure source 61, the RO water flows out of the RO water chamber 42 and the diaphragm pump 45a (45b). ). The RO water that has flowed out of the RO water chamber 42 is supplied to the heat exchanger 400 through the flow path 302. The RO water supplied to the heat exchanger 400 is subjected to heat exchange while passing through the tube members 401 to 403 of the heat exchanger 400. Thereby, the temperature is adjusted so that the temperature of the RO water approaches the ambient temperature. The temperature-controlled RO water is drawn into the diaphragm pump 45a (45b). Subsequently, the valves 208 and 213 are closed, the valves 214 and 209 (210) are opened, and the RO water drawn into the diaphragm pump 45a (45b) by the positive pressure applied by the positive pressure source 62 is supplied to the first dilution chamber 43 ( The second dilution chamber 44) is supplied. Such RO water is supplied 24 times by the diaphragm pumps 45 a and 45 b, whereby 288 mL of RO water whose temperature is adjusted is supplied to the first dilution chamber 43. Similarly, 12 mL of high concentration reagent is supplied from the high concentration reagent chamber 41 to the first dilution chamber 43, and approximately 300 mL of RO water and high concentration reagent are supplied by the diaphragm pumps 45 a and 45 b.

  In step S <b> 6, the total amount of about 300 mL of the mixed solution stored in the first dilution chamber 43 (or the second dilution chamber 44) is supplied to the stirring chamber 46. At this time, the supplied mixed liquid is stirred in the stirring chamber 46 by flowing along the inner wall of the stirring chamber 46 by a pipe 461 provided in the stirring chamber 46. Further, for example, while the mixed solution is supplied from the first dilution chamber 43 to the stirring chamber 46, about 300 mL of the mixed solution is supplied to the second dilution chamber 44 in step S5. Therefore, the first dilution chamber 43 and the second dilution chamber 44 include a supply operation of the reagent (mixed liquid) to the stirring chamber 46 and a dilution operation in which a total of about 300 mL of RO water and a high concentration reagent are supplied. Are performed alternately.

In step S7, the electromagnetic valves 218 and 219 are opened, and the reagent is supplied (transferred) from the stirring chamber 46 toward the supply chamber 47. At this time, in step S8, the electrical conductance measuring unit 410, with electrical conductance Z _T of the reagent passing through the reagent flow path 418 (see FIG. 4) is measured, the temperature T of the reagent is measured by the thermistor 417 The

Next, in step S9, the CPU 48a performs temperature compensation to the reference temperature (25 ° C.) based on the measured reagent temperature T and electrical conductivity Z _T (corresponding to T ° C.), whereby the electric charge of the reagent is obtained. Conductivity _Zr (25 degreeC conversion value) is calculated. In step S10, the CPU 48a determines whether or not the electrical conductivity Z _r (25 ° C. converted value) of the reagent is within a predetermined range. That is, the 25 ° C. converted value Z _{r of} the measured electrical conductivity Z _T is a predetermined range with respect to the target value Z ₀ of the electrical conductivity of the reagent at a dilution factor of 25 times measured in advance and at a reference temperature (25 ° C.). It is determined whether it is within.

If the electrical conductivity Z _r (25 ° C. converted value) is not within the predetermined range, the electromagnetic valve 219 (see FIG. 2) is closed and the electromagnetic valve 221 (see FIG. 2) is opened in step S11. The reagent in the stirring chamber 46 is discarded from the disposal port 502. When the electrical conductivity Z _r (25 ° C. converted value) is within a predetermined range, the electromagnetic valve 219 (see FIG. 2) is opened (the electromagnetic valve 221 is closed), so that the reagent in the stirring chamber 46 is removed. It is supplied to the supply chamber 47. As a result, only the reagent diluted with high accuracy can be stored in the supply chamber 47. When the float switch 105 detects that the reagent in the stirring chamber 46 is empty due to the reagent being supplied from the stirring chamber 46 to the supply chamber 47 or being discarded from the disposal port 502, the second The reagent is supplied from the dilution chamber 44 (or the first dilution chamber 43) to the stirring chamber 46.

  Next, in step S12, the CPU 48a determines whether or not there is a shutdown instruction from the user. If there is no instruction, the process proceeds to step S4. Therefore, when there is no shutdown instruction in step S12, the processes from steps S4 to S12 are repeated. During the processing from step S4 to step S12, the measurement unit 2 performs the sample measurement operation (use of the reagent). For this reason, in parallel with the processing from step S4 to S12, the reagent in the supply chamber 47 is measured by the negative pressure supplied from the negative pressure source 81 (see FIG. 1) of the pneumatic unit 8 (see FIG. 1). Suctioned to the unit 2 (supplied to the measuring unit 2). Further, as described in steps S5 to S7 above, the high-concentration reagent, the RO water, the high-concentration reagent chamber 41, the RO water chamber 42, the first dilution chamber 43, the second dilution chamber 44, and the stirring chamber 46, respectively. Supply of a liquid mixture etc. is performed based on the detection result of each float switch 100-105. Therefore, when the liquid in each chamber is empty, the operation is performed in the order of steps S4 to S7. In the subsequent operations, the float switch 100 is used regardless of the determination result of the reagent amount in the supply chamber 47 in step S4. The supply operation to each chamber is performed at the timing when it is determined that the supply of each liquid is necessary based on the detection results of ˜105.

  If there is a shutdown instruction, a predetermined shutdown process is executed in step S13. For example, when there is a reagent being prepared, the operation is continued until it is finally supplied to the supply chamber 47. Further, the supply of the RO water to the RO water chamber 42 is completed, and the RO water is discharged from the RO water chamber 42. Thereby, it is possible to prevent the RO water from staying in the RO water chamber 42 until the reagent preparation device 4 is started next time. When the shutdown process is normally performed, the reagent preparation process operation ends.

In the present embodiment, as described above, it is passed through the reagent supplied from the stirring chamber 46 (mixed solution) in the interior, by providing the electrical conductance measuring unit 410 for obtaining the electrical conductivity of Z _T of the reagent passing Since the liquid level of the liquid (reagent) in the stirring chamber 46 does not come into contact with the electric conductivity measuring unit 410, the electric conductivity measuring unit 410 when bubbles are generated on the liquid level in the stirring chamber 46 is obtained. The influence of can be suppressed. Therefore, for example, the mixed solution may be stirred without providing a propeller in the tank, and the configuration of the reagent preparing device 4 can be simplified. Further, a thermistor 417 for acquiring the liquid temperature of the reagent is provided, and a heat exchanger 400 for adjusting the temperature of the liquid is provided upstream of the thermistor 417 and the electrical conductivity measuring unit 410, so that the temperature-adjusted reagent is thermistor. 417 and electrical conductivity measurement unit 410 can be supplied. Thereby, the temperature difference between the liquid temperature of the mixed liquid when the electric conductivity Z _T is measured by the electric conductivity measuring unit 410 and the liquid temperature T (° C.) acquired by the thermistor 417 can be reduced. The flow of the reagent can be controlled based on the accurate electric conductivity _Zr .

  Further, in the present embodiment, the heat exchanger 400 is provided with pipe members 401, 402, and 403 having thermal conductivity, and RO water is passed through the pipe members 401, 402, and 403 to heat the RO water and the outside. By configuring so that the exchange can be performed, the supplied RO water is passed through the inside of the tube members 401, 402, and 403 having thermal conductivity, thereby exchanging heat between the RO water and the outside (atmosphere). Therefore, the liquid temperature of the RO water can be easily adjusted with a simple configuration without using a configuration such as a temperature sensor or a heater.

  In the present embodiment, the tube members 401, 402, and 403 having thermal conductivity are made of metal (SUS316L). By configuring the tube members 401, 402, and 403 with such a metal (SUS316L) that has high thermal conductivity and is easy to process, heat exchange between the RO water and the outside (atmosphere) can be performed effectively. Tube members 401, 402, and 403 having high thermal conductivity can be easily obtained.

  In this embodiment, the diaphragm pumps 45a and 45b supply a larger amount of RO water (about 288 ml) than the high concentration reagent (about 12 ml) to the first dilution chamber 43 or the second dilution chamber 44 (stirring chamber 46). By configuring as described above, it is possible to adjust the temperature of the reagent (mixed liquid) only by adjusting the temperature of the RO water, so there is no need to provide a temperature adjusting unit for adjusting the temperature of the high concentration reagent, The configuration can be simplified.

  Further, in the present embodiment, the heat exchanger 400 is washed after the RO water is supplied to the heat exchanger 400 by configuring the first liquid of the present invention to be RO water (pure water). There is no need.

Further, in the present embodiment, the control unit 48 (the CPU 48a), electrical conductivity _{Z r} of the reagent at a reference temperature (25 ° C.), it is determined whether within a predetermined range with respect to the target value _{Z 0,} electrical When the conductivity Z _r is not within the predetermined range with respect to the target value Z ₀ , the reagent quality (concentration) is controlled by adjusting the reagent flow so that the reagent is discarded from the disposal port 502. Since the low-quality reagent that is determined based on the electrical conductivity _Zr and is not within the predetermined range can be discarded from the disposal port 502, only the reagent (mixed solution) having an appropriate concentration that does not cause a quality problem is measured. Can be supplied to.

In the present embodiment, the thermistor 417 is disposed inside the electrical conductivity measurement unit 410, so that the liquid temperature when the electrical conductivity Z _T is acquired by the electrical conductivity measurement unit 410 and the thermistor 417. Deviation from the obtained liquid temperature T (° C.) becomes smaller.

  The embodiment disclosed this time should be considered as illustrative in all points and not restrictive. The scope of the present invention is shown not by the above description of the embodiments but by the scope of claims for patent, and further includes all modifications within the meaning and scope equivalent to the scope of claims for patent.

  For example, in the above-described embodiment, the reagent preparation device 4 installed separately from the measurement unit 2 is shown as an example of the reagent preparation device of the present invention, but the present invention is not limited to this. The reagent preparation device of the present invention may be, for example, a reagent preparation device that is provided in a measurement unit and functions as a reagent preparation mechanism. Examples of the measurement unit (apparatus) having a reagent preparation mechanism include a blood cell counter, an immunoassay apparatus, and a smear preparation apparatus. The present invention may be applied to these reagent preparation mechanisms.

  Moreover, in the said one Embodiment, although the example which provided the RO water preparation part 7 in the exterior of the reagent preparation apparatus 4 was shown, this invention is not limited to this. In the present invention, the RO water preparation unit may be provided inside the reagent preparation device as a part of the reagent preparation device. Also in this case, a heat exchanger may be provided downstream of the intake port that takes in RO water from the RO water production unit.

  In the above embodiment, the example in which the RO water chamber 42 for storing the RO water supplied to the first dilution chamber 43 (second dilution chamber 44) is shown. However, the present invention is not limited to this. I can't. In this invention, you may comprise so that RO water may be directly supplied to a dilution chamber by a diaphragm pump from an RO water preparation part, without providing RO water chamber.

  In the above embodiment, an example in which two dilution chambers (the first dilution chamber 43 and the second dilution chamber 44) are provided as an example of the mixing container of the present invention is shown. However, the present invention is not limited to this. Absent. In the present invention, only one mixing container (dilution chamber) may be provided, or three or more mixing containers (dilution chambers) may be provided.

  Moreover, in the said one Embodiment, although the example which provided the stirring chamber 46 was shown as an example of the mixing container of this invention, this invention is not limited to this. In the present invention, the stirring chamber need not be provided. If the dilution chamber (the first dilution chamber 43 and the second dilution chamber 44) is configured to reliably mix (stir) the RO water and the high concentration reagent, it is not necessary to provide a stirring chamber. Therefore, in this case, the electric conductivity measuring unit 410 may be provided on the downstream side of the dilution chamber.

  Moreover, in the said one Embodiment, although the example which provided the heat exchanger 400 was shown as an example of the temperature control part of this invention, this invention is not limited to this. In the present invention, a heater and a cooler other than the heat exchanger may be used as the temperature adjustment unit. In this case, the liquid temperature and the atmospheric temperature may be acquired by a thermistor, and the temperature of the liquid temperature may be adjusted by a heater and a cooler so that the two coincide.

  In the above embodiment, the heat exchanger 400 is provided between the RO water chamber 42 and the dilution chamber (the first dilution chamber 43 and the second dilution chamber 44) (the flow path 302). The present invention is not limited to this. In the present invention, the position between the intake port 500 and the RO water chamber 42 (flow path 501), the position between the dilution chamber (the first dilution chamber 43 and the second dilution chamber 44) and the stirring chamber 46, and The heat exchanger may be provided at any position such as a position between the stirring chamber 46 and the electric conductivity measuring unit 410. The heat exchanger should just be provided in the upstream rather than the electrical conductivity measurement unit as an electrical conductivity acquisition part of this invention, and the thermistor as a liquid temperature acquisition part of this invention. In the case where a heat exchanger is provided at a position between the dilution chamber and the stirring chamber, or between the stirring chamber and the electric conductivity measurement unit, the reagent after dilution (mixing) is used instead of the RO water. The temperature of the mixed solution) is adjusted. The present invention may have such a configuration.

  In the above embodiment, an example in which one heat exchanger 400 is provided between the RO water chamber 42 and the dilution chamber (the first dilution chamber 43 and the second dilution chamber 44) (the flow path 302) is shown. However, the present invention is not limited to this. In the present invention, two or more heat exchangers may be provided.

  In the above embodiment, the heat exchanger 400 is provided with three heat conductive tube members 401, 402, and 403, but the present invention is not limited to this. One or two pipe members having thermal conductivity may be used, or four or more pipe members may be used.

  Moreover, in the said one Embodiment, although the tubular member 401, 402, and 403 which has heat conductivity showed the example comprised with the metal (SUS316L), respectively, this invention is not limited to this. In the present invention, the tube member may be made of a material other than metal, or may be made of a metal material other than SUS316L, such as aluminum.

  In the above-described embodiment, the heat exchanger 400 uses the heat conductive pipe members 401, 402, and 403. However, the present invention is not limited to this. In this invention, you may use members other than a pipe member for a heat exchanger.

  In the present invention, the installation location of the heat exchanger is not particularly limited. Therefore, in the present invention, the heat exchanger may be housed inside the reagent preparing device 4 or may be provided so as to be exposed to the outside of the device.

  In the above embodiment, an example in which the electrical conductivity measurement unit 410 is provided between the stirring chamber 46 and the supply chamber 47 (a flow path from the stirring chamber 46 to the supply chamber 47) has been described. Is not limited to this. The electrical conductivity measuring unit need not be provided between the stirring chamber and the supply chamber. For example, it may be provided in a flow path different from the flow path from the stirring chamber to the supply chamber. In this case, a part of the reagent in the stirring chamber may be supplied to the electrical conductivity measurement unit, and if the electrical conductivity is within a predetermined range, the residual liquid in the stirring chamber may be supplied to the supply chamber. Good.

  In the above-described embodiment, an example in which the electrode 414, the electrode 415, and the electrode 416 are provided in the electrical conductivity measurement unit 410 has been described. However, the present invention is not limited to this. In the present invention, an electrical conductivity measurement unit provided with two (a pair of) electrodes may be used. Further, the electrodes 414 and 415 at both ends of the electrical conductivity measuring unit 410 may be connected to an AC power source, and the center electrode 415 may be grounded. Moreover, you may provide four or more electrodes in an electrical conductivity measurement unit.

  Moreover, in the said one Embodiment, although the example which provided the thermistor 417 in the inside of the electrical conductivity measurement unit 410 was shown as an example of the liquid temperature acquisition part of this invention, this invention is not limited to this. In the present invention, the thermistor may be provided outside the electrical conductivity measurement unit.

  In the above embodiment, the thermistor 417 is disposed on the second measurement region 410b side in the electrical conductivity measurement unit 410. However, the present invention is not limited to this. In the present invention, the thermistor 417 may be provided on the first measurement region 410 a side of the electrical conductivity measurement unit 410 or may be provided at another position inside the electrical conductivity measurement unit 410.

  Moreover, in the said one Embodiment, although the example which used the thermistor 417 was shown as an example of the liquid temperature acquisition part of this invention, this invention is not limited to this. In the present invention, a temperature sensor other than the thermistor may be used for the liquid temperature acquisition unit.

2 Measurement Unit 4 Reagent Preparation Device 43 First Dilution Chamber (Mixing Container)
44 Second dilution chamber (mixing vessel)
45a, 45b Diaphragm pump (quantitative supply unit)
46 Stirring chamber (stirring section)
48 Control unit 400 Heat exchanger (temperature control unit)
401, 402, 403 Pipe member 410 Electrical conductivity measurement unit (electrical conductivity acquisition unit)
417 Thermistor (Liquid temperature acquisition unit)
418 Reagent flow path 502 Waste port Z _T conductivity (electric conductivity of the mixture)

Claims (11)

A reagent preparation device configured to be able to supply a mixed liquid containing a first liquid and a second liquid as a reagent to a measurement unit that measures a specimen using a reagent,
A mixing container for mixing the first liquid and the second liquid;
Electric conductivity acquisition that is provided downstream from the mixing container, allows the liquid mixture supplied from the mixing container to pass through an internal reagent channel, and acquires the electric conductivity of the liquid mixture that passes through the reagent channel. And
A liquid temperature acquisition unit that is provided downstream from the mixing container and acquires the liquid temperature of the mixed liquid;
Provided upstream from the electrical conductivity acquisition unit and the liquid temperature acquisition unit, the temperature of at least one of the first liquid, the second liquid, and the mixed liquid deviates from the ambient temperature. In this case, by adjusting the temperature of the liquid so as to be close to the ambient temperature, the temperature of the mixed liquid passing through the electrical conductivity acquisition unit and the liquid temperature acquisition unit is adjusted so that the liquid temperature of the mixed liquid approaches the ambient temperature. A temperature control unit to suppress ,
A reagent preparation device comprising: a controller that controls the flow of the mixed liquid based on the electrical conductivity acquired by the electrical conductivity acquisition unit and the liquid temperature acquired by the liquid temperature acquisition unit. The temperature adjusting unit includes a pipe member having thermal conductivity, and allows the liquid to pass through the pipe member by allowing the liquid to pass through the pipe member and exchanging heat between the liquid and the outside of the pipe member. The reagent preparation device according to claim 1, wherein the temperature is adjusted to be close to the ambient temperature around the temperature control unit .   The reagent preparing device according to claim 2, wherein the tube member is configured so that the liquid passes through the inside while changing the direction.   The reagent preparing device according to claim 2 or 3, wherein the thermally conductive tube member is made of metal.   The reagent preparation device according to any one of claims 1 to 4, wherein the temperature adjustment unit is provided upstream of the mixing container and configured to adjust the temperature of the first liquid.   The reagent preparing device according to claim 5, wherein the first liquid is pure water. A quantitative supply unit for quantitatively supplying the first liquid and the second liquid to the mixing container;
The reagent preparation device according to claim 5 or 6, wherein the quantitative supply unit is configured to supply a larger amount of the first liquid than the second liquid to the mixing container. A waste port for discarding the mixture;
The control unit determines whether or not the liquid mixture satisfies a predetermined condition based on the electric conductivity acquired by the electric conductivity acquisition unit and the liquid temperature acquired by the liquid temperature acquisition unit. The reagent preparing device according to any one of claims 1 to 7, wherein the reagent preparing device is configured to control a flow of the mixed solution so that the mixed solution flows to the disposal port when a predetermined condition is not satisfied.   The reagent preparation apparatus according to claim 1, wherein the liquid temperature acquisition unit is provided inside the electrical conductivity acquisition unit. The reagent channel has a predetermined length,
The reagent preparation device according to claim 9, wherein the liquid temperature acquisition unit is provided at a position away from a central position of the reagent flow path of the electrical conductivity acquisition unit.   The reagent preparation device according to any one of claims 1 to 10, further comprising a stirring unit that stirs the mixed liquid by flowing into the container.

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