JP5535681B2 - Skin disinfecting composition - Google Patents
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- JP5535681B2 JP5535681B2 JP2010037702A JP2010037702A JP5535681B2 JP 5535681 B2 JP5535681 B2 JP 5535681B2 JP 2010037702 A JP2010037702 A JP 2010037702A JP 2010037702 A JP2010037702 A JP 2010037702A JP 5535681 B2 JP5535681 B2 JP 5535681B2
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- 239000000203 mixture Substances 0.000 title claims description 143
- 230000000249 desinfective effect Effects 0.000 title description 51
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 113
- 239000007788 liquid Substances 0.000 claims description 76
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 48
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 37
- 238000004659 sterilization and disinfection Methods 0.000 claims description 22
- 125000003158 alcohol group Chemical group 0.000 claims description 3
- 239000003921 oil Substances 0.000 description 112
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- 210000003491 skin Anatomy 0.000 description 34
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- 238000011156 evaluation Methods 0.000 description 8
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- 239000000645 desinfectant Substances 0.000 description 6
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- GVJHHUAWPYXKBD-IEOSBIPESA-N α-tocopherol Chemical compound OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-IEOSBIPESA-N 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
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- 239000003760 tallow Substances 0.000 description 2
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 description 1
- PZNPLUBHRSSFHT-RRHRGVEJSA-N 1-hexadecanoyl-2-octadecanoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCCCC(=O)O[C@@H](COP([O-])(=O)OCC[N+](C)(C)C)COC(=O)CCCCCCCCCCCCCCC PZNPLUBHRSSFHT-RRHRGVEJSA-N 0.000 description 1
- SPSPIUSUWPLVKD-UHFFFAOYSA-N 2,3-dibutyl-6-methylphenol Chemical compound CCCCC1=CC=C(C)C(O)=C1CCCC SPSPIUSUWPLVKD-UHFFFAOYSA-N 0.000 description 1
- LOYINFBFPXYKQD-UHFFFAOYSA-N 2-butyl-3-methoxyphenol Chemical compound CCCCC1=C(O)C=CC=C1OC LOYINFBFPXYKQD-UHFFFAOYSA-N 0.000 description 1
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- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 239000003349 gelling agent Substances 0.000 description 1
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- 150000002632 lipids Chemical class 0.000 description 1
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- 239000000244 polyoxyethylene sorbitan monooleate Substances 0.000 description 1
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- 235000003441 saturated fatty acids Nutrition 0.000 description 1
- 150000004671 saturated fatty acids Chemical class 0.000 description 1
- 210000002374 sebum Anatomy 0.000 description 1
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- 235000002316 solid fats Nutrition 0.000 description 1
- 239000008347 soybean phospholipid Substances 0.000 description 1
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- Medicinal Preparation (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Description
本発明は、消毒作用及び保湿作用を有する皮膚消毒用組成物に関する。 The present invention relates to a skin disinfecting composition having a disinfecting action and a moisturizing action.
従来から、手指の消毒や感染症等の防止のため、病院や一般家庭などで使用される皮膚用消毒液が種々提案されている。これらは殺菌成分を含む消毒液と、殺菌成分を含まない高濃度アルコールを含むアルコール系消毒液とがある。
このうち、アルコール系消毒液は、高濃度アルコールによる殺菌力を有し、人体に対する刺激がそれほど大きくないため、抵抗力の弱い幼児や老人へも安心して使用することができるため広く受け入れられている。また、アルコール系消毒液は速乾性があるため、頻繁に手指の洗浄・消毒を行う必要がある医療関係者や介護関係者等の簡易的な消毒の場合には、アルコール系消毒液が適している。
Conventionally, various skin disinfectants have been proposed for use in hospitals and general homes in order to disinfect fingers and prevent infections. These include a disinfecting solution containing a sterilizing component and an alcohol-based disinfecting solution containing a high concentration alcohol not containing a sterilizing component.
Among them, alcohol-based disinfectants are widely accepted because they have a bactericidal power due to high-concentration alcohol and are not so irritating to the human body, so they can be used with peace of mind even for infants and the elderly with weak resistance. . Also, since alcohol-based disinfectants are quick-drying, alcohol-based disinfectants are suitable for simple disinfection of medical personnel and caregivers who frequently need to wash and disinfect hands. Yes.
このようなアルコール系消毒液は、高濃度（５０質量％以上）のエタノールやイソプロパノールなどを消毒作用の主成分とし、必要に応じて他成分を添加したものである（例えば、特許文献１参照）。しかしながら、高濃度アルコールは、消毒効果は高いものの、脱脂作用が強く、皮膚から皮脂流失の原因となり、頻繁に使用すると肌荒れを生じさせる。
そのため、このような消毒用組成物には皮膚刺激性の低減や、すすいだ後の肌感触の改善を目的として、保湿剤を添加することが多い。例えば、特許文献２には、保湿成分としてポリエチレングリコール、プロピレングリコール及びブチレングルコールを使用した消毒用組成物が開示されている。
Such an alcohol-based disinfectant has a high concentration (50% by mass or more) of ethanol or isopropanol as a main component of the disinfecting action, and other components are added as necessary (see, for example, Patent Document 1). . However, although high-concentration alcohol has a high disinfecting effect, it has a strong degreasing action and causes sebum loss from the skin. If it is frequently used, it causes rough skin.
Therefore, a moisturizing agent is often added to such a disinfecting composition for the purpose of reducing skin irritation and improving skin feel after rinsing. For example, Patent Document 2 discloses a disinfecting composition using polyethylene glycol, propylene glycol and butylene glycol as moisturizing components.
しかしながら、上述の消毒用組成物に含まれる従来の石油などを原料とする保湿成分は、アレルギーの原因となることがある。そのため、日常に高頻度に使用される、消毒用アルコールの添加物としては、必ずしも適当とはいえなかった。一方で、動物性油脂、植物性油脂由来の保湿成分は、消毒用アルコールとの混合が困難であり、保存時における経時安定性を保つことができないと考えられてきた。 However, a conventional moisturizing component made from petroleum or the like contained in the disinfecting composition described above may cause allergies. Therefore, it is not necessarily suitable as an additive for alcohol for disinfection that is frequently used in daily life. On the other hand, it has been considered that moisturizing ingredients derived from animal fats and vegetable fats and oils are difficult to mix with disinfecting alcohol and cannot maintain stability over time during storage.
かかる状況下、本発明の目的は、十分な消毒作用と保湿作用を有し、皮膚に対する刺激感が小さい皮膚消毒用組成物を提供することである。 Under such circumstances, an object of the present invention is to provide a skin disinfecting composition having a sufficient disinfecting action and a moisturizing action and having a small irritation to the skin.
本発明者は、人の角質層の細胞間脂質に近く優れたスキンケア成分である馬油に注目して、消毒用アルコールへの配合を試みた。従来、疎水性である馬油は、アルコールとの混和性が低く、エタノールなどの消毒用アルコールへの混合は、乳化剤を多量に使用する以外不可能であり、消毒用アルコールの保湿成分としては不適当と考えられてきた。本発明者は、鋭意研究を重ねた結果、原料馬脂を精製処理した液状馬油と、イソプロパノールとが乳化剤を使用せずとも十分な相溶性を有することを見出した。そして、液状馬油とイソプロパノール及び水分の割合を適当な範囲とすることによって、上記目的に達成できることを見出し、本発明に至った。 The present inventor has focused on horse oil, which is an excellent skin care ingredient close to the intercellular lipids of the human stratum corneum, and tried to blend it into alcohol for disinfection. Conventionally, horse oil, which is hydrophobic, has low miscibility with alcohol, and mixing with disinfecting alcohol such as ethanol is impossible except by using a large amount of emulsifier, and is not a moisturizing component of disinfecting alcohol. It has been considered appropriate. As a result of intensive studies, the present inventor has found that liquid horse oil obtained by refining raw material tallow and isopropanol have sufficient compatibility without using an emulsifier. And it discovered that it could achieve the said objective by making the ratio of liquid horse oil, isopropanol, and a water | moisture content into a suitable range, and came to this invention.
すなわち、本発明は、以下の発明に係るものである。
＜１＞ 液状馬油（Ａ）０．１〜１．６質量％、イソプロパノールを必須成分として含むアルコール組成物（Ｂ）７９〜９９質量％（但し、アルコール組成物（Ｂ）の組成中、イソプロパノールの割合が、６０質量％以上である。）、及び、水（Ｃ）１〜２０質量％、を含有する皮膚消毒用組成物。
（但し、液状馬油（Ａ）、アルコール組成物（Ｂ）及び水（Ｃ）の合計を１００質量％とする。）
＜２＞ 液状馬油（Ａ）の曇点が、７℃以下である前記＜１＞記載の皮膚消毒用組成物。
＜３＞ 液状馬油（Ａ）の曇点が、−２℃以下である前記＜１＞記載の皮膚消毒用組成物。
＜４＞ アルコール組成物（Ｂ）が、エタノール２５〜４０質量％を含有する前記＜１＞から＜３＞のいずれかに記載の皮膚消毒用組成物。
＜５＞ アルコール組成物（Ｂ）が、イソプロパノール６０〜７５質量％と、エタノール２５〜４０質量％からなる前記＜１＞から＜３＞のいずれかに記載の皮膚消毒用組成物。
That is, the present invention relates to the following inventions.
<1> 0.1 to 1.6% by mass of liquid horse oil (A), 79 to 99% by mass of an alcohol composition (B) containing isopropanol as an essential component (however, isopropanol is included in the composition of the alcohol composition (B)) The composition for skin disinfection containing the water (C) 1-20 mass%.
(However, the total of liquid horse oil (A), alcohol composition (B) and water (C) is 100% by mass.)
<2> The skin disinfecting composition according to <1>, wherein the cloud point of the liquid horse oil (A) is 7 ° C. or lower.
<3> The skin disinfecting composition according to <1>, wherein the cloud point of the liquid horse oil (A) is −2 ° C. or lower.
<4> The composition for skin disinfection according to any one of <1> to <3>, wherein the alcohol composition (B) contains 25 to 40% by mass of ethanol.
<5> The composition for skin disinfection according to any one of <1> to <3>, wherein the alcohol composition (B) comprises 60 to 75% by mass of isopropanol and 25 to 40% by mass of ethanol.
本発明によると、精製した液状馬油により、保湿力に優れ、肌への刺激感や使用後のかさつき感が少ない皮膚消毒用組成物が提供される。 According to the present invention, a purified liquid horse oil provides a skin disinfecting composition having excellent moisturizing power and less irritation to the skin and a feeling of bulkiness after use.
本発明は、液状馬油（Ａ）０．１〜１．６質量％、イソプロパノールを必須成分として含むアルコール組成物（Ｂ）７９〜９９質量％（但し、アルコール組成物（Ｂ）の組成中、イソプロパノールの割合が、６０質量％以上である。）、及び、水（Ｃ）１〜２０質量％、を含有する皮膚消毒用組成物（但し、液状馬油（Ａ）、アルコール組成物（Ｂ）及び水（Ｃ）の合計を１００質量％とする。）に係るものである。
以下、詳細に説明する。
The present invention is a liquid horse oil (A) 0.1-1.6% by mass, an alcohol composition (B) 79-99% by mass containing isopropanol as an essential component (however, during the composition of the alcohol composition (B), And a composition for skin disinfection containing 1 to 20% by mass of water (C) (however, liquid horse oil (A), alcohol composition (B)) And the total of water (C) is 100 mass%).
Details will be described below.
液状馬油（Ａ）は、馬脂をレンダリングして溶出した油分を脱ガム、脱酸、脱臭、分別を行うことによって得られる液体の油であり、本発明の皮膚消毒用組成物において保湿性を付与する作用がある。液状馬油（Ａ）は、公知の方法、例えば、特開平３−１２８３０８号公報に記載の製造方法に準じる方法で得た精製馬油を分別して得ることができる。 The liquid horse oil (A) is a liquid oil obtained by degumming, deoxidizing, deodorizing and separating the oil eluted by rendering horse fat, and is moisturizing in the skin disinfecting composition of the present invention. Has the effect of imparting Liquid horse oil (A) can be obtained by fractionating purified horse oil obtained by a known method, for example, a method according to the production method described in JP-A-3-128308.
簡単に説明すると、所定量の馬の脂身（原料馬脂）をレンダリング用クッカーに入れ、減圧下、８０℃〜１３０℃程度の温度条件にて撹拌し、溶出した油分を分取する。レンダリングは乾式でも湿式でもよい。得られた油分をリン酸処理装置で減圧下、９０℃程度で撹拌しながら所定量のリン酸を添加し、所定の時間処理することにより生じた凝固ガム質を遠心分離して脱ガムを行う。次いで、脱ガム後の原油をアルカリ処理槽にて希薄苛性ソーダにて遊離脂肪酸を除去し、更に活性白土を添加して色素を吸着させた後にろ過する。さらにろ過して得られた液体を脱臭塔におくり、水蒸気蒸留を行うことによって有臭揮発成分を除去し、精製馬油（原液）を得る。 Briefly, a predetermined amount of horse fat (raw material horse fat) is put into a rendering cooker and stirred under a reduced pressure at a temperature of about 80 ° C. to 130 ° C. to separate the eluted oil. The rendering may be dry or wet. A predetermined amount of phosphoric acid is added to the obtained oil component while stirring at about 90 ° C. under reduced pressure in a phosphoric acid treatment device, and the solidified gum resulting from the treatment for a predetermined time is centrifuged to perform degumming. . Next, the crude oil after degumming is filtered after removing free fatty acids with dilute caustic soda in an alkali treatment tank, and further adding activated clay to adsorb the pigment. Further, the liquid obtained by filtration is placed in a deodorizing tower and subjected to steam distillation to remove odorous volatile components, thereby obtaining purified horse oil (stock solution).
次いで、精製馬油のウィンタリングを行う。ウィンタリングは、液状油を冷却することにより液状油から飽和脂肪酸等の固脂を分離することである。具体的には、精製馬油を所定の温度で所定の時間（３〜５日程度）静置することで液状油と、固脂を分離することができる。 Next, the purified horse oil is wintered. Wintering is the separation of solid fats such as saturated fatty acids from liquid oil by cooling the liquid oil. Specifically, liquid oil and solid fat can be separated by allowing purified horse oil to stand at a predetermined temperature for a predetermined time (about 3 to 5 days).
上述のウィンタリングにおける温度設定によって、得られる液状馬油（Ａ）の曇点、すなわち、液状馬油から固形成分の析出する温度が決定される。なお、曇点は、日本油化学会制定の基準油脂分析試験法に準拠した方法によって測定することができる。
この曇点が、気温を超えると、最終品である皮膚消毒用組成物においても馬油由来の固形成分が析出するおそれがある。そのため、液状馬油（Ａ）は、曇点が７℃以下であることが好ましく、特に１℃以下であることが好ましく、中でも−２℃以下であることが好ましい。曇点が７℃を超えると、低温（７℃以下）に実質的に馬油成分が析出することがあるため、馬油の添加効果が得られない場合がある。
特に曇点が１℃以下であると、本発明の皮膚消毒用組成物から、より低温（３℃程度）まで馬油成分が析出せず、析出した場合においても温度を上げると容易に溶解する。さらに、曇点が−２℃以下であると、実質的に０℃まで使用できるため特に好適である。
なお、液状馬油（Ａ）の曇点の下限値には特に限定はなく、曇点が低温であるほど、より低温の環境下でも固化した馬油の析出がおこらないため好適である。一方で、曇点が低温であるほど原料馬脂から分取できる量が少なくなるため、本発明の消毒用組成物に使用される液状馬油（Ａ）の曇点の下限値は、−４℃程度で十分である。
The cloud point of the obtained liquid horse oil (A), that is, the temperature at which the solid component is precipitated from the liquid horse oil is determined by the temperature setting in the above-described wintering. In addition, a cloud point can be measured by the method based on the standard oil-fat analysis test method established by the Japan Oil Chemical Society.
If the cloud point exceeds the air temperature, the horse oil-derived solid component may be deposited in the final skin disinfecting composition. Therefore, the liquid horse oil (A) preferably has a cloud point of 7 ° C. or less, particularly preferably 1 ° C. or less, and particularly preferably −2 ° C. or less. When the cloud point exceeds 7 ° C., the horse oil component may be substantially precipitated at a low temperature (7 ° C. or lower), so that the effect of adding horse oil may not be obtained.
In particular, when the cloud point is 1 ° C. or lower, the horse oil component does not precipitate from the skin disinfecting composition of the present invention to a lower temperature (about 3 ° C.), and even when it is precipitated, it dissolves easily when the temperature is raised. . Furthermore, it is particularly preferable that the cloud point is −2 ° C. or lower because it can be used up to 0 ° C.
The lower limit of the cloud point of the liquid horse oil (A) is not particularly limited, and the lower the cloud point, the more suitable the solidified horse oil does not precipitate even in a lower temperature environment. On the other hand, the lower the cloud point, the smaller the amount that can be separated from the raw tallow, so the lower limit of the cloud point of the liquid horse oil (A) used in the disinfecting composition of the present invention is -4. A temperature of about ° C is sufficient.
液状馬油（Ａ）の割合は、皮膚消毒用組成物全量に対して、０．１〜１．６質量％であり、０．５〜１質量％がより好ましい。０．１質量％未満であると、保湿作用が不十分となり、１．６質量％を超えると、液状馬油（Ａ）が分離するため好ましくない。 The ratio of liquid horse oil (A) is 0.1-1.6 mass% with respect to the skin disinfecting composition whole quantity, and 0.5-1 mass% is more preferable. If it is less than 0.1% by mass, the moisturizing action is insufficient, and if it exceeds 1.6% by mass, the liquid horse oil (A) is separated, which is not preferable.
液状馬油（Ａ）には、品質劣化の抑制のため、酸化防止剤が添加されていることが好ましい。酸化防止剤としては、トコフェロール（ビタミンＥ）、２−ブチル−ヒドロキシアニソール（ＢＨＡ）、ジブチルヒドロキシトルエン（ＢＨＴ）などが挙げられ、トコフェロールが特に好適である。酸化防止剤の添加量は、液状馬油（Ａ）に対して、通常、０．０１〜０．１質量％、好ましくは、０．０２〜０．０６質量％である。 It is preferable that an antioxidant is added to the liquid horse oil (A) for the purpose of suppressing quality deterioration. Examples of the antioxidant include tocopherol (vitamin E), 2-butyl-hydroxyanisole (BHA), dibutylhydroxytoluene (BHT) and the like, and tocopherol is particularly suitable. The addition amount of antioxidant is 0.01-0.1 mass% normally with respect to liquid horse oil (A), Preferably, it is 0.02-0.06 mass%.
アルコール組成物（Ｂ）は、イソプロパノール（別名：２−プロパノール）を６０質量％以上含有するアルコール組成物である。イソプロパノールは、消毒作用を有し、かつ、上記液状馬油（Ａ）を溶解することができるアルコールである。なお、液状馬油（Ａ）の溶解安定性を向上させるという観点からは、アルコール組成物（Ｂ）中のイソプロパノールの割合は、７０質量％以上であることが好ましい。 The alcohol composition (B) is an alcohol composition containing 60% by mass or more of isopropanol (also called 2-propanol). Isopropanol is an alcohol that has a disinfecting action and can dissolve the liquid horse oil (A). In addition, from the viewpoint of improving the dissolution stability of the liquid horse oil (A), the proportion of isopropanol in the alcohol composition (B) is preferably 70% by mass or more.
また、アルコール組成物（Ｂ）は、エタノールを含んでいることが好ましい。エタノールは、イソプロパノールより強い消毒作用を有すアルコールであり、親水性が高いため、単独では馬油を十分量溶解できないものの、アルコール組成物（Ｂ）の一成分として、所定の割合をイソプロパノールと混合することができる。
アルコール組成物（Ｂ）に対するエタノールの割合は、２５〜４０質量％であることが好ましい。エタノールのアルコール組成物（Ｂ）に対する割合が、２５質量％未満であると、エタノールを添加したことによる消毒作用の向上効果が顕著に認められない。また、エタノールは、親水性が強く、馬油の溶解性が小さいため、４０質量％を超えると、馬油成分が分離しやすくなるため好ましくない。
Moreover, it is preferable that the alcohol composition (B) contains ethanol. Ethanol is an alcohol that has a stronger disinfecting effect than isopropanol, and because it is highly hydrophilic, horse oil cannot be dissolved in a sufficient amount by itself, but a predetermined proportion is mixed with isopropanol as a component of the alcohol composition (B). can do.
The ratio of ethanol to the alcohol composition (B) is preferably 25 to 40% by mass. When the ratio of ethanol to the alcohol composition (B) is less than 25% by mass, the effect of improving the disinfection action due to the addition of ethanol is not recognized. Moreover, since ethanol has strong hydrophilicity and low solubility of horse oil, it is not preferable to exceed 40% by mass because the horse oil component is easily separated.
なお、アルコール組成物（Ｂ）は、イソプロパノールやエタノール以外の他のアルコールを含んでいてもよい。他のアルコールとして、具体的には、メタノール、１−プロパノール、１−ブタノール、２−ブタノール、ポリエチレングリコール、ポリビニルアルコール、グリセリンなどが挙げられる。
一方で、他のアルコールは、イソプロパノール、エタノールと比較して、消毒作用が弱く、また、安全性が懸念されるものも含まれる。そのため、アルコール組成物（Ｂ）は、 アルコール組成物（Ｂ）が、イソプロパノール６０〜７５質量％と、エタノール２５〜４０質量％からなり、他のアルコールを含有しないことが好ましい。
そして、他のアルコールを含有する場合にもアルコール組成物（Ｂ）に対する割合として、１５質量％以下であることが好ましい。
In addition, the alcohol composition (B) may contain alcohol other than isopropanol and ethanol. Specific examples of other alcohols include methanol, 1-propanol, 1-butanol, 2-butanol, polyethylene glycol, polyvinyl alcohol, and glycerin.
On the other hand, other alcohols have weaker disinfecting effects than isopropanol and ethanol, and include those for which safety is a concern. Therefore, the alcohol composition (B) is preferably composed of 60 to 75% by mass of isopropanol and 25 to 40% by mass of ethanol, and does not contain other alcohols.
And also when containing other alcohol, it is preferable that it is 15 mass% or less as a ratio with respect to an alcohol composition (B).
アルコール組成物（Ｂ）の割合は、皮膚消毒用組成物全量に対して、７９〜９９質量％であり、８３〜９５質量％がより好ましい。７９質量％未満であると、消毒作用が不十分となり、また、液状馬油（Ａ）が分離しやすくなる。一方で、アルコール組成物（Ｂ）におけるイソプロパノール（及びエタノール）の消毒作用は、水（Ｃ）が存在することによって増強されるため、アルコール組成物（Ｂ）の割合が、９９質量％を超えると消毒作用が不十分となる場合がある。 The ratio of the alcohol composition (B) is 79 to 99% by mass, and more preferably 83 to 95% by mass with respect to the total amount of the skin disinfecting composition. If it is less than 79% by mass, the disinfection action becomes insufficient and the liquid horse oil (A) is easily separated. On the other hand, since the disinfection action of isopropanol (and ethanol) in the alcohol composition (B) is enhanced by the presence of water (C), when the proportion of the alcohol composition (B) exceeds 99% by mass. The disinfection action may be insufficient.
水（Ｃ）は、本発明の皮膚消毒用組成物に保湿性を与えると共に、上述のようにイソプロパノール（及びエタノール）の消毒作用を増強する目的で配合される。その割合は、皮膚消毒用組成物全量に対して、１〜２０質量％であり、４〜８質量％がより好ましい。水（Ｃ）の割合が、１質量％未満であると、保湿性および消毒作用が不十分となり、２０質量％を超えると、液状馬油（Ａ）が分離するため好ましくない。 Water (C) is blended for the purpose of imparting moisture retention to the skin disinfecting composition of the present invention and enhancing the disinfecting action of isopropanol (and ethanol) as described above. The ratio is 1-20 mass% with respect to the skin disinfecting composition whole quantity, and 4-8 mass% is more preferable. If the ratio of water (C) is less than 1% by mass, the moisture retention and disinfection action will be insufficient, and if it exceeds 20% by mass, the liquid horse oil (A) will be separated, which is not preferable.
また、本発明の皮膚消毒用組成物には、本発明の効果を損なわない範囲で通常皮膚消毒用組成物や皮膚外用医薬などで使用される任意の成分を含有することができる。これら任意成分の配合割合は、その目的に応じて適宜選択して決定することができる。例えば、従来公知の消毒殺菌剤、増粘・ゲル化剤、酸化防止剤、色素剤、防腐剤、ｐＨ調整剤、香料等が挙げられる。 Moreover, the skin disinfecting composition of the present invention can contain any component that is usually used in a skin disinfecting composition, a skin external medicine, or the like as long as the effects of the present invention are not impaired. The blending ratio of these optional components can be selected and determined as appropriate according to the purpose. For example, conventionally known disinfecting disinfectants, thickening / gelling agents, antioxidants, coloring agents, preservatives, pH adjusting agents, fragrances and the like can be mentioned.
本発明で用いる皮膚消毒用組成物は、所定量の上記原料成分を混合することにより製造することができる。
混合方法は任意であるが、実質的に液状馬油（Ａ）が均一になるまで行われる。混合時間は、原料組成、特に液状馬油（Ａ）の物性に依存するが、数分から数日程度である。
製造時の液状馬油（Ａ）の分離を抑制し、また、混合時間を短縮するために、アルコール組成物（Ｂ）の成分であるイソプロパノールに液状馬油（Ａ）を溶解させたのちに、残りの成分（アルコール組成物（Ｂ）の残りアルコール成分及び水（Ｃ））を添加して混合する方法が好ましい。
The skin disinfecting composition used in the present invention can be produced by mixing a predetermined amount of the above raw material components.
The mixing method is arbitrary, but is performed until the liquid horse oil (A) is substantially uniform. The mixing time depends on the raw material composition, particularly the physical properties of the liquid horse oil (A), but is about several minutes to several days.
In order to suppress separation of the liquid horse oil (A) during production and to shorten the mixing time, after dissolving the liquid horse oil (A) in isopropanol which is a component of the alcohol composition (B), A method of adding and mixing the remaining components (the remaining alcohol component of the alcohol composition (B) and water (C)) is preferable.
混合温度は、馬油成分が析出しない温度、すなわち、液状馬油（Ａ）の曇点以上の温度であればよい。上限温度は特に制限はないが、アルコール組成物（Ｂ）の揮発による減少を抑制するために、６０℃以下が好ましく、好適には４０℃以下であり、通常、室温である。 The mixing temperature may be a temperature at which the horse oil component does not precipitate, that is, a temperature not lower than the cloud point of the liquid horse oil (A). Although there is no restriction | limiting in particular in upper limit temperature, in order to suppress the reduction | decrease by volatilization of alcohol composition (B), 60 degrees C or less is preferable, It is 40 degrees C or less suitably, and it is room temperature normally.
本発明の皮膚消毒用組成物は、皮膚に塗布する際にのびがよく、保湿力に優れ、肌への刺激感や使用後のかさつき感が少ない。液状馬油（Ａ）の曇点未満の温度で保管すると、油脂成分の結晶析出があるが、常温まで戻し攪拌することにより速やかに再溶解する。 The composition for skin disinfection of the present invention has good stretchability when applied to the skin, is excellent in moisturizing power, and has little irritation to the skin and a feeling of bulkiness after use. When stored at a temperature lower than the cloud point of the liquid horse oil (A), there is crystallization of the oil and fat component, but it is quickly re-dissolved by stirring back to room temperature.
以下、実施例により本発明を更に詳細に説明するが、本発明の要旨を越えない限り以下の実施例に限定されるものではない。 EXAMPLES Hereinafter, although an Example demonstrates this invention further in detail, unless it exceeds the summary of this invention, it is not limited to a following example.
使用した試薬は次の通りである。
「試薬」
・エタノール（和光純薬工業株式会社製、324-00037）
・２−プロパノール（和光純薬工業株式会社製、166-04836）
・グリセリン脂肪酸エステル（理研ビタミン株式会社製、ポエム B-10）
・ショ糖脂肪酸エステル（三菱化学フーズ株式会社製、リョートーシュガーエステル P-1670）
・グリセリン（日油株式会社、食添グリセリン）
The reagents used are as follows.
"reagent"
・ Ethanol (Wako Pure Chemical Industries, 324-00037)
・ 2-propanol (Wako Pure Chemical Industries, 166-04836)
・ Glycerin fatty acid ester (Riken Vitamin Co., Ltd., Poem B-10)
・ Sucrose fatty acid ester (Mitsubishi Chemical Foods, Ryoto Sugar Ester P-1670)
・ Glycerin (NOF Co., Ltd., dietary glycerin)
「精製液状馬油の製造」
液状馬油は特開平３−１２８３０８号公報で開示された方法に準拠した方法にて、製造した。すなわち、所定量の馬の脂身をレンダリング用クッカーに入れ、真空−５００ｍｍHgG、温度１０５℃〜１１０℃の条件にて撹拌し、溶出した油を遠心分離機で分取した。得られた液体をリン酸処理装置で真空−６００ｍｍHｇG、温度９３℃の条件で撹拌しながらリン酸を０．０３％添加し、所定の時間処理することにより生じた凝固ガム質を遠心分離して脱ガムを行った。
次いで、脱ガム後の原油をアルカリ処理槽にて希薄苛性ソーダにて遊離脂肪酸を除去し、更に活性白土を添加して色素を吸着させた後にろ過した。さらにろ過して得られた液体を脱臭塔におくり、温度２５０℃、真空度３Ｔｏｒｒ（ａｂｓ．）にて水蒸気蒸留を行うことによって有臭揮発成分を除去し、精製馬油（原液）を得た。なお、精製馬油（原液）には酸化防止剤としてトコフェロールを適当量添加した。
"Manufacture of refined liquid horse oil"
Liquid horse oil was produced by a method based on the method disclosed in JP-A-3-128308. That is, a predetermined amount of horse fat was placed in a rendering cooker, stirred under the conditions of vacuum-500 mmHgG, temperature 105 ° C. to 110 ° C., and the eluted oil was collected by a centrifuge. While stirring the obtained liquid in a phosphoric acid treatment apparatus under the conditions of vacuum-600 mmHgG and temperature of 93 ° C., 0.03% of phosphoric acid is added, and the solidified gum resulting from the treatment for a predetermined time is centrifuged. Degumming was performed.
Next, the crude oil after degumming was filtered after removing free fatty acids with dilute caustic soda in an alkali treatment tank, and further adding activated clay to adsorb the pigment. Further, the liquid obtained by filtration was placed in a deodorization tower, and odorous volatile components were removed by steam distillation at a temperature of 250 ° C. and a degree of vacuum of 3 Torr (abs.) To obtain a purified horse oil (stock solution). . An appropriate amount of tocopherol was added as an antioxidant to the purified horse oil (stock solution).
次いで、精製馬油（原液）のウィンタリングを行った。
（分別１回目）
精製馬油（原液）を室温（２３℃）にて４日間攪拌（５ｒｐｍ）したのちにろ過し、液状油と固脂に分離した。なお、分別１回後に得られた液状油の曇点は、６．７℃であった。
（分別２回目）
得られた液状油を、１８℃にて４日間攪拌（５ｒｐｍ）したのちにろ過し、液状油と固脂に分離した。なお、分別２回後に得られた液状油の曇点は、０．８℃であった。
（分別３回目）
得られた液状油を、７℃にて５日間攪拌（５ｒｐｍ）したのちにろ過し、液状油と固脂に分離した。なお、分別３回後に得られた液状油の曇点は、０．１℃であった。
（分別４回目）
得られた液状油を、３℃にて３日間攪拌（５ｒｐｍ）したのちにろ過し、液状油と固脂に分離した。なお、分別４回後に得られた液状油の曇点は、−４．０℃であった。
Subsequently, wintering of refined horse oil (stock solution) was performed.
(First separation)
Purified horse oil (stock solution) was stirred (5 rpm) at room temperature (23 ° C.) for 4 days and then filtered to separate into liquid oil and solid fat. The cloud point of the liquid oil obtained after one fractionation was 6.7 ° C.
(Second separation)
The obtained liquid oil was stirred (5 rpm) at 18 ° C. for 4 days and then filtered to separate into liquid oil and solid fat. The cloud point of the liquid oil obtained after the second fractionation was 0.8 ° C.
(3rd separation)
The obtained liquid oil was stirred (5 rpm) at 7 ° C. for 5 days and then filtered to separate into liquid oil and solid fat. In addition, the cloud point of the liquid oil obtained after 3 times of fractionation was 0.1 degreeC.
(4th separation)
The obtained liquid oil was stirred (5 rpm) at 3 ° C. for 3 days and then filtered to separate into liquid oil and solid fat. In addition, the cloud point of the liquid oil obtained after 4 times of fractionation was -4.0 degreeC.
次いで、分別４回後に得られた液状油を、真空度８Ｔｏｒｒ（ａｂｓ．）、温度２２０℃の条件にて水蒸気蒸留を行い、蒸留されずに残った液体に適当量のトコフェロールを添加することによって、目的物である液状馬油を得た。作製した液状馬油及び上記試薬を用いて下記の消毒用組成物を製造した。 Next, the liquid oil obtained after the fourth separation is subjected to steam distillation under the conditions of a vacuum degree of 8 Torr (abs.) And a temperature of 220 ° C., and an appropriate amount of tocopherol is added to the remaining liquid without distillation. As a result, liquid horse oil, which was the target product, was obtained. The following disinfecting composition was produced using the produced liquid horse oil and the above reagents.
（試験例１）
室温（約２３℃）にて、所定の容器に入れたＩＰＡ８４．８重量部に対して、液状馬油０．５重量部を添加し、攪拌機（新東科学株式会社製、型番：スリーワンモータ BL600）を用いて、２００ｒｐｍにて５分間撹拌混合した。次いで、水１４．７重量部を添加し、同攪拌機で５分撹拌することによって、試験例１の組成物を得た。該組成物は、白濁のない均一な溶液であり、２４時間室温放置した後も均一溶液のままであり、油分の分離は確認されなかった。表１に組成、結果を示す。
(Test Example 1)
At room temperature (about 23 ° C), 0.5 parts by weight of liquid horse oil is added to 84.8 parts by weight of IPA in a predetermined container, and a stirrer (manufactured by Shinto Kagaku Co., Ltd., model number: Three-One Motor BL600). ) And stirred and mixed at 200 rpm for 5 minutes. Next, 14.7 parts by weight of water was added, and the composition of Test Example 1 was obtained by stirring for 5 minutes with the same stirrer. The composition was a uniform solution without white turbidity, and remained a uniform solution after being allowed to stand at room temperature for 24 hours, and separation of oil was not confirmed. Table 1 shows the composition and results.
（試験例２）
原料組成を、ＩＰＡ７９．８重量部、精製液状馬油０．５重量部、水１９．７重量部に変更した以外は、試験例１と同様にして、試験例２の組成物を得た。該組成物は、うっすらと白濁がみられたものの均一な溶液であり、２４時間室温放置した後も均一溶液のままであり、油分の分離は確認されなかった。表１に組成、結果を示す。
(Test Example 2)
A composition of Test Example 2 was obtained in the same manner as Test Example 1 except that the raw material composition was changed to 79.8 parts by weight of IPA, 0.5 parts by weight of purified liquid horse oil, and 19.7 parts by weight of water. The composition was a homogeneous solution with slight turbidity, and remained a uniform solution after being allowed to stand at room temperature for 24 hours, and separation of oil was not confirmed. Table 1 shows the composition and results.
（試験例３）
原料組成を、ＩＰＡ６９．８重量部、精製液状馬油０．５重量部、水２９．７重量部に変更した以外は、試験例１と同様にして、試験例３の組成物を得た。該組成物は、白濁し透明にならなかった。表１に組成、結果を示す。
(Test Example 3)
The composition of Test Example 3 was obtained in the same manner as in Test Example 1, except that the raw material composition was changed to 69.8 parts by weight of IPA, 0.5 part by weight of purified liquid horse oil, and 29.7 parts by weight of water. The composition was cloudy and did not become transparent. Table 1 shows the composition and results.
（試験例４）
室温（約２３℃）にて、所定の容器に入れたＩＰＡ９３．４重量部に対して、液状馬油０．５重量部を添加し、攪拌機（新東科学株式会社製、型番：スリーワンモータ BL600）を用いて、２００ｒｐｍにて５分間撹拌混合した。次いで、エタノール５．０重量部及び水１．１重量部を添加し、同攪拌機で５分撹拌することによって、試験例４の組成物を得た。該組成物は、白濁のない均一な溶液であり、２４時間室温放置した後も均一溶液のままであり、油分の分離は確認されなかった。表１に組成、結果を示す。
(Test Example 4)
At room temperature (about 23 ° C), 0.5 parts by weight of liquid horse oil is added to 93.4 parts by weight of IPA in a predetermined container, and a stirrer (manufactured by Shinto Kagaku Co., Ltd., model number: Three-One Motor BL600). ) And stirred and mixed at 200 rpm for 5 minutes. Subsequently, 5.0 parts by weight of ethanol and 1.1 parts by weight of water were added, and the composition of Test Example 4 was obtained by stirring for 5 minutes with the same stirrer. The composition was a uniform solution without white turbidity, and remained a uniform solution after being allowed to stand at room temperature for 24 hours, and separation of oil was not confirmed. Table 1 shows the composition and results.
（試験例５）
原料組成を、ＩＰＡ６６．５重量部、精製液状馬油０．５重量部、エタノール２６．４重量部、水６．６重量部に変更した以外は、試験例４と同様にして、試験例５の組成物を得た。該組成物は、白濁のない均一な溶液であり、２４時間室温放置した後も均一溶液のままであり、油分の分離は確認されなかった。表１に組成、結果を示す。
(Test Example 5)
Test Example 5 was carried out in the same manner as Test Example 4 except that the raw material composition was changed to 66.5 parts by weight of IPA, 0.5 parts by weight of purified liquid horse oil, 26.4 parts by weight of ethanol, and 6.6 parts by weight of water. A composition was obtained. The composition was a uniform solution without white turbidity, and remained a uniform solution after being allowed to stand at room temperature for 24 hours, and separation of oil was not confirmed. Table 1 shows the composition and results.
（試験例６）
原料組成を、ＩＰＡ６３．９重量部、精製液状馬油０．５重量部、エタノール２５．６重量部、水１０．０重量部に変更した以外は、試験例４と同様にして、試験例６の組成物を得た。該組成物は、うっすらと白濁がみられたものの均一な溶液であり、２４時間室温放置した後も均一溶液のままであり、油分の分離は確認されなかった。表１に組成、結果を示す。
(Test Example 6)
Test Example 6 was performed in the same manner as Test Example 4 except that the raw material composition was changed to 63.9 parts by weight of IPA, 0.5 parts by weight of purified liquid horse oil, 25.6 parts by weight of ethanol, and 10.0 parts by weight of water. A composition was obtained. The composition was a homogeneous solution with slight turbidity, and remained a uniform solution after being allowed to stand at room temperature for 24 hours, and separation of oil was not confirmed. Table 1 shows the composition and results.
（試験例７）
原料組成を、ＩＰＡ６６．１重量部、精製液状馬油０．８重量部、エタノール２６．５重量部、水６．６重量部に変更した以外は、試験例４と同様にして、試験例７の組成物を得た。該組成物は、白濁のない均一な溶液であり、２４時間室温放置した後も均一溶液のままであり、油分の分離は確認されなかった。表１に組成、結果を示す。
(Test Example 7)
Test Example 7 was carried out in the same manner as in Test Example 4 except that the raw material composition was changed to 66.1 parts by weight of IPA, 0.8 part by weight of purified liquid horse oil, 26.5 parts by weight of ethanol, and 6.6 parts by weight of water. A composition was obtained. The composition was a uniform solution without white turbidity, and remained a uniform solution after being allowed to stand at room temperature for 24 hours, and separation of oil was not confirmed. Table 1 shows the composition and results.
（試験例８）
原料組成を、ＩＰＡ６６．０重量部、精製液状馬油１．０重量部、エタノール２６．４重量部及び水６．６重量部に変更した以外は、試験例４と同様にして、試験例８の組成物を得た。該組成物は、白濁のない均一な溶液であり、２４時間室温放置した後も均一溶液のままであり、油分の分離は確認されなかった。表１に組成、結果を示す。
(Test Example 8)
Test Example 8 was carried out in the same manner as in Test Example 4 except that the raw material composition was changed to 66.0 parts by weight of IPA, 1.0 part by weight of purified liquid horse oil, 26.4 parts by weight of ethanol and 6.6 parts by weight of water. A composition was obtained. The composition was a uniform solution without white turbidity, and remained a uniform solution after being allowed to stand at room temperature for 24 hours, and separation of oil was not confirmed. Table 1 shows the composition and results.
（試験例９）
原料組成を、ＩＰＡ６６．０重量部、精製液状馬油１．５重量部、エタノール２６．０重量部及び水６．５重量部に変更した以外は、試験例４と同様にして、試験例９の組成物を得た。該組成物は、若干白濁したもののほぼ均一な溶液であり、２４時間室温放置した後も均一溶液のままであり、水分と油分との明確な分離は確認されなかった。表１に組成、結果を示す。
(Test Example 9)
Test Example 9 was conducted in the same manner as in Test Example 4 except that the raw material composition was changed to 66.0 parts by weight of IPA, 1.5 parts by weight of purified liquid horse oil, 26.0 parts by weight of ethanol and 6.5 parts by weight of water. A composition was obtained. Although the composition was slightly turbid, it was an almost uniform solution and remained a uniform solution even after being allowed to stand at room temperature for 24 hours, and no clear separation of water and oil was confirmed. Table 1 shows the composition and results.
（試験例１０）
原料組成を、ＩＰＡ６５．５重量部、精製液状馬油２．０重量部、エタノール２６．０重量部及び水６．５重量部に変更した以外は、試験例４と同様にして、試験例１０の組成物を得た。該組成物は、白濁し透明にならなかった。表１に組成、結果を示す。
(Test Example 10)
Test Example 10 was carried out in the same manner as Test Example 4 except that the raw material composition was changed to 65.5 parts by weight of IPA, 2.0 parts by weight of purified liquid horse oil, 26.0 parts by weight of ethanol and 6.5 parts by weight of water. A composition was obtained. The composition was cloudy and did not become transparent. Table 1 shows the composition and results.
（試験例１１）
原料組成を、ＩＰＡ５９．７重量部、精製液状馬油０．５重量部、エタノール３１．８重量部及び水８．０重量部に変更した以外は、試験例４と同様にして、試験例１１の組成物を得た。該組成物は、白濁のない均一な溶液であり、２４時間室温放置した後も均一溶液のままであり、油分の分離は確認されなかった。表１に組成、結果を示す。
(Test Example 11)
Test Example 11 was conducted in the same manner as in Test Example 4 except that the raw material composition was changed to 59.7 parts by weight of IPA, 0.5 parts by weight of purified liquid horse oil, 31.8 parts by weight of ethanol and 8.0 parts by weight of water. A composition was obtained. The composition was a uniform solution without white turbidity, and remained a uniform solution after being allowed to stand at room temperature for 24 hours, and separation of oil was not confirmed. Table 1 shows the composition and results.
（試験例１２）
原料組成を、ＩＰＡ５４．７重量部、精製液状馬油０．５重量部、エタノール３５．８重量部及び水９．０重量部に変更した以外は、試験例４と同様にして、試験例１２の組成物を得た。該組成物は、若干白濁したもののほぼ均一な溶液であり、２４時間室温放置した後も均一溶液のままであり、水分と油分との明確な分離は確認されなかった。表１に組成、結果を示す。
(Test Example 12)
Test Example 12 was conducted in the same manner as in Test Example 4 except that the raw material composition was changed to 54.7 parts by weight of IPA, 0.5 parts by weight of purified liquid horse oil, 35.8 parts by weight of ethanol, and 9.0 parts by weight of water. A composition was obtained. Although the composition was slightly turbid, it was an almost uniform solution and remained a uniform solution even after being allowed to stand at room temperature for 24 hours, and no clear separation of water and oil was confirmed. Table 1 shows the composition and results.
（試験例１３）
原料組成を、ＩＰＡ４９．７重量部、精製液状馬油０．５重量部、エタノール３９．８重量部及び水１０．０重量部に変更した以外は、試験例４と同様にして、試験例１３の組成物を得た。該組成物は、白濁し透明にならなかった。表１に組成、結果を示す。
(Test Example 13)
Test Example 13 was conducted in the same manner as in Test Example 4 except that the raw material composition was changed to 49.7 parts by weight of IPA, 0.5 parts by weight of purified liquid horse oil, 39.8 parts by weight of ethanol, and 10.0 parts by weight of water. A composition was obtained. The composition was cloudy and did not become transparent. Table 1 shows the composition and results.
（試験例１４）
原料組成を、ＩＰＡ３０．０重量部、精製液状馬油０．５重量部、エタノール５５．６重量部及び水１３．９重量部に変更した以外は、試験例４と同様にして、試験例１４の組成物を得た。該組成物は、若干乳化するもの水分と油分の分離が確認された。表１に組成、結果を示す
(Test Example 14)
Test Example 14 was carried out in the same manner as Test Example 4 except that the raw material composition was changed to 30.0 parts by weight of IPA, 0.5 parts by weight of purified liquid horse oil, 55.6 parts by weight of ethanol and 13.9 parts by weight of water. A composition was obtained. Although the composition was slightly emulsified, separation of water and oil was confirmed. Table 1 shows the composition and results.
（試験例１５）
原料組成を、ＩＰＡ２０．０重量部、精製液状馬油０．５重量部、エタノール６３．６重量部及び水１５．９重量部に変更した以外は、試験例４と同様にして、試験例１５の組成物を得た。該組成物は、ほとんど乳化せず、水分と油分とが分離していた。
表１に組成、結果を示す。
(Test Example 15)
Test Example 15 was conducted in the same manner as in Test Example 4 except that the raw material composition was changed to 20.0 parts by weight of IPA, 0.5 parts by weight of purified liquid horse oil, 63.6 parts by weight of ethanol, and 15.9 parts by weight of water. A composition was obtained. The composition was hardly emulsified, and water and oil were separated.
Table 1 shows the composition and results.
上記試験例の消毒用組成物のうち、白濁、分離を起こさなかった消毒用組成物について、以下の消毒性評価および保湿性評価を行った。 Of the disinfecting compositions of the above test examples, disinfecting compositions that did not cause white turbidity and separation were subjected to the following disinfection evaluation and moisturizing evaluation.
「消毒性評価」
黄色ブドウ球菌（Staphylococcus aureus）あるいはＯ−１５７（腸管出血性大腸菌）をＳＣＤ寒天培地にて３０℃、２４時間培養した後に、各培養菌を滅菌生理食塩水に懸濁し、約１０8個/ｍＬに調整した試験菌液を作製した。各試験菌液を、プラスチック基板に接種した後、被接種表面に、上記試験例の消毒用組成物をそれぞれスプレー塗布しサンプルとした。また、本発明の消毒用組成物の代わりに水をスプレー塗布し、対照とした。
２時間後、サンプル及び対照の基板からサンプリングし、それを寒天培地上で培養した際のコロニー形成状況により、それぞれの消毒用組成物の消毒効果を評価した。
結果を表１に併せて示す。なお、表１において、評価基準は以下の通りである。
（評価基準）
○：ほとんどコロニーの形成が確認されない
△：若干コロニーを形成するが対照と比較して明らかに少ない
×：対照と同程度にコロニーが形成する
評価を行ったすべての消毒用組成物が消毒作用を示し、本発明の消毒用組成物は、アルコールの消毒作用を保持していることが確認された。特にエタノールを含む試験例は、強い消毒作用を示した。
"Disinfection evaluation"
30 ° C. Staphylococcus aureus (Staphylococcus aureus) or O-157 (enterohemorrhagic Escherichia coli) in SCD agar medium after culturing for 24 hours, were suspended each culture in sterile physiological saline, about 10 8 cells / mL A test bacterial solution adjusted to 1 was prepared. After each test bacterial solution was inoculated on a plastic substrate, the disinfecting composition of the above-mentioned test example was sprayed on the surface to be inoculated to prepare a sample. Further, instead of the disinfecting composition of the present invention, water was spray applied as a control.
Two hours later, the sample and the control substrate were sampled, and the disinfecting effect of each disinfecting composition was evaluated based on the colony formation state when the sample was cultured on the agar medium.
The results are also shown in Table 1. In Table 1, the evaluation criteria are as follows.
(Evaluation criteria)
○: Almost no formation of colonies Δ: Some colonies are formed but clearly less compared to controls ×: Colonies are formed to the same extent as controls
All of the evaluated disinfecting compositions exhibited a disinfecting action, and it was confirmed that the disinfecting composition of the present invention retained the disinfecting action of alcohol. In particular, test examples containing ethanol showed a strong disinfecting action.
「保湿性評価」
パネラー２０人に各消毒用組成物を使用してもらい、以下の基準で保湿効果を評価した。結果を表１に併せて示す。なお、評価結果はパネラーの平均点により示した。
（評価基準）
ほとんど手が荒れず保湿効果を感じた：２点
若干の保湿効果を感じた：１点
保湿効果を感じない：０点
評価を行ったすべての消毒用組成物が１．０点以上であり、本発明の消毒用組成物は、保湿作用を有していることが確認された。
"Moisturizing evaluation"
20 panelists used each disinfecting composition and evaluated the moisturizing effect according to the following criteria. The results are also shown in Table 1. In addition, the evaluation result was shown by the average score of the panel.
(Evaluation criteria)
I felt moisturizing effect with almost no hands rough: 2 points I felt a little moisturizing effect: 1 point No moisturizing effect: 0 points
All the disinfecting compositions evaluated were 1.0 points or more, and it was confirmed that the disinfecting composition of the present invention has a moisturizing action.
本発明の皮膚消毒用組成物の消毒性をより詳細に評価するために、試験例５の組成物について、殺菌効力試験を行った。試験条件は以下の通りである。
（１）試験菌
黄色ブドウ球菌（Staphylococcus aureus、NBRC13276）
緑膿菌（Pseudomonas aeruginosa、NBRC13275）
ＭＲＳＡ（メチシリン耐性黄色ブドウ球菌、IID1677）
Ｏ−１５７（腸管出血性大腸菌、RIMD0509939）
（２）試験菌液の調整
各試験菌を寒天培地にて３０℃、２４時間培養した後に、各培養菌を滅菌生理食塩水に懸濁し、約１０8個/ｍＬに調整した試験菌液を作製した。
（３）殺菌効力試験
あらかじめ２５℃の恒温に保存した検体（試験例５の組成物）１９．８ｇを滅菌バイアル瓶にとり、これに試験菌液を１％量（０．２ｍＬ）接種した。接種後、１５秒、３０秒、６０秒、１２０秒後に１ｇサンプリングして、ＬＰ希釈液（大豆レシチン：０．７ｇ、ポリソルベート８０：２０ｇ、ペプトン：１ｇ、精製水１０００ｍＬからなる液体）９ｍＬにて希釈する。得られた希釈液を段階希釈し、寒天平板混釈法によって生菌数を測定した。なお、生菌数の測定培地はＳＣＤＬＰ寒天培地を使用し、培養条件は、３０℃、３日間である。
試験結果を表２に示す。表２から評価したすべての菌が１５秒以内に実質的に殺菌されており、本発明の皮膚消毒用組成物が高い殺菌性を有することが確認された。
In order to evaluate the disinfection of the skin disinfecting composition of the present invention in more detail, the composition of Test Example 5 was subjected to a bactericidal efficacy test. The test conditions are as follows.
(1) Test bacteria Staphylococcus aureus (NBRC13276)
Pseudomonas aeruginosa (Pseudomonas aeruginosa, NBRC13275)
MRSA (methicillin-resistant Staphylococcus aureus, IID1677)
O-157 (Enterohemorrhagic Escherichia coli, RIMD0509939)
(2) Preparation of test bacterial solution After culturing each test bacterium in an agar medium at 30 ° C for 24 hours, each culture was suspended in sterile physiological saline, and the test microbial solution adjusted to about 10 8 cells / mL was prepared. Produced.
(3) Bactericidal efficacy test 19.8 g of the specimen (the composition of Test Example 5) previously stored at a constant temperature of 25 ° C. was placed in a sterile vial, and 1% amount (0.2 mL) of the test bacterial solution was inoculated therein. After inoculation, sample 1 g after 15 seconds, 30 seconds, 60 seconds and 120 seconds, and with 9 mL of LP diluent (soy lecithin: 0.7 g, polysorbate 80:20 g, peptone: 1 g, liquid consisting of 1000 mL of purified water) Dilute. The obtained diluted solution was serially diluted, and the viable cell count was measured by an agar plate pour method. The measuring medium for viable cell count is SCDLP agar medium, and the culture conditions are 30 ° C. and 3 days.
The test results are shown in Table 2. All the bacteria evaluated from Table 2 were substantially sterilized within 15 seconds, and it was confirmed that the skin disinfecting composition of the present invention has high bactericidal properties.
参考例として、乳化剤を使用して馬油とアルコールを混合した例を示す。
（参考例１）
室温（約２３℃）にて、所定の容器にエタノール７９．２重量部、液状馬油０．９７５重量部、水１９．８重量部及び乳化剤としてグリセリン脂肪酸エステル０．００２５重量部を入れ、ホモジナイザー（JANKE＆KUNKEL社製、型番：ULTRA-TORRAX T25）を用いて、１００００ｒｐｍにて１０分間撹拌混合することによって、参考例１の組成物を得た。
該組成物は、乳化はするが安定ではなく、混合直後から油滴が浮かんでいた。さらに時間の経過と共に水分と油分とが分離した。
As a reference example, an example in which horse oil and alcohol are mixed using an emulsifier is shown.
(Reference Example 1)
At room temperature (about 23 ° C.), put 79.2 parts by weight of ethanol, 0.975 parts by weight of liquid horse oil, 19.8 parts by weight of water and 0.0025 parts by weight of glycerin fatty acid ester as an emulsifier in a predetermined container. The composition of Reference Example 1 was obtained by stirring and mixing at 10000 rpm for 10 minutes using (manufactured by JANKE & KUNKEL, model number: ULTRA-TORRAX T25).
The composition was emulsified but not stable, and oil droplets floated immediately after mixing. Furthermore, water and oil were separated with time.
（参考例２）
参考例１原料において、さらに乳化剤であるショ糖脂肪酸エステル０．２５重量部を添加した以外は参考例１と同様にして、参考例２の組成物を得た。
該組成物は、乳化はするが参考例１と比較すると若干安定ではあるが十分でなく、時間の経過と共に水分と油分とが分離した。
(Reference Example 2)
Reference Example 1 A composition of Reference Example 2 was obtained in the same manner as Reference Example 1, except that 0.25 parts by weight of sucrose fatty acid ester as an emulsifier was further added.
Although the composition was emulsified, it was slightly stable compared to Reference Example 1 but was not sufficient, and water and oil separated with time.
（参考例３）
室温（約２３℃）にて、所定の容器にエタノール７５．６重量部、液状馬油０．５重量部、水１８．９重量部及びグリセリン５．０重量部を入れ、ホモジナイザー（JANKE＆KUNKEL社製、型番：ULTRA-TORRAX T25）を用いて、１００００ｒｐｍにて１０分間撹拌混合することによって、参考例３の組成物を得た。
該組成物は、乳化はするが参考例１と比較すると若干安定ではあるが十分でなく、混合直後には油滴がほとんどなかったものの、５時間後には油分と水分が分離し油滴の生成が認された。
(Reference Example 3)
At room temperature (about 23 ° C), put 75.6 parts by weight of ethanol, 0.5 parts by weight of liquid horse oil, 18.9 parts by weight of water and 5.0 parts by weight of glycerin in a predetermined container, and homogenizer (manufactured by JANKE & KUNKEL) The composition of Reference Example 3 was obtained by stirring and mixing at 10,000 rpm for 10 minutes using a model number: ULTRA-TORRAX T25).
The composition is emulsified but slightly stable as compared to Reference Example 1, but is not sufficient, and there were almost no oil droplets immediately after mixing, but after 5 hours the oil and water separated, producing oil droplets. Was recognized.
本発明は、保湿力に優れ、十分な消毒作用を有するため、皮膚に対する刺激感が小さい皮膚洗浄用組成物として有用である。 INDUSTRIAL APPLICATION Since this invention is excellent in moisture retention and has sufficient disinfection action, it is useful as a skin washing composition with little irritation | stimulation feeling with respect to skin.
Claims (5)
イソプロパノールを必須成分として含むアルコール組成物（Ｂ）７９〜９９質量％（但し、アルコール組成物（Ｂ）の組成中、イソプロパノールの割合が、６０質量％以上である。）、
及び、水（Ｃ）１〜２０質量％、を含有することを特徴とする皮膚消毒用組成物。
（但し、液状馬油（Ａ）、アルコール組成物（Ｂ）及び水（Ｃ）の合計を１００質量％とする。） Liquid horse oil (A) 0.1-1.6% by mass,
79 to 99% by mass of an alcohol composition (B) containing isopropanol as an essential component (however, in the composition of the alcohol composition (B), the proportion of isopropanol is 60% by mass or more),
And the composition for skin disinfection characterized by containing 1-20 mass% of water (C).
(However, the total of liquid horse oil (A), alcohol composition (B) and water (C) is 100% by mass.)
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| JPH03264513A (en) * | 1990-03-14 | 1991-11-25 | Tokyo Erugu:Kk | Emulsifable composition |
| JPH1053790A (en) * | 1996-08-09 | 1998-02-24 | Haba Shokai:Kk | Liquid horse oil and its production |
| JP4754206B2 (en) * | 2004-11-25 | 2011-08-24 | エコラボ株式会社 | Disinfectant composition |
| WO2008121355A1 (en) * | 2007-03-29 | 2008-10-09 | International Flora Technologies, Ltd. | Management of dermatitic symptoms of mammalian integument with emollient disinfectant formulations |
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