JP5507718B2 - Disinfection method against avian influenza virus - Google Patents

Description

  The present invention relates to a method for disinfecting an avian influenza virus by inactivating a bird flu virus by bringing a drug containing the liquid as an active ingredient into contact with an object to be disinfected, and also used as a safe disinfectant containing the liquid as an active ingredient. An avian influenza virus inactivating agent is provided.

In recent years, the increase in viral diseases has been remarkable, and SARS (Severe Acute Respiratory Syndrome) and avian influenza have become a worldwide threat, and the speed and scale of infection is a threat to mankind.
Avian influenza is a disease of birds, including poultry, caused by avian influenza virus infection. Chickens are broadly classified into low pathogenic viruses and highly pathogenic viruses based on their disease state, and particularly high pathogenic viruses have a high mortality rate against chickens. Recent major outbreaks are the United States, Australia, Italy, Hong Kong, North Korea, etc., and the occurrence of H5N1 subtype was confirmed in Asian countries (Japan, Korea, Vietnam, etc.) from 2003 to 2004. Outbreaks continued in 2005 and in several Southeast Asian countries, and migratory bird infections due to the highly toxic H5N1 virus have been confirmed in Mongolia, Russia and other countries since 2005.

Originally, the host range of viruses is limited, but avian influenza virus is a virus with a wide host range that can infect not only birds but also mammals. Recently, some viruses have been reported to infect humans directly in exceptional cases. In 1997, 18 people in Hong Kong were infected-6 people were killed. In 2003, two people were infected in Fujian Province-1 people were killed. 2003 There are civic infections in the Netherlands in 1974, Vietnam in 2004-2006, Thailand, Cambodia, Indonesia, etc.
Since avian influenza virus is transported to remote areas by migratory birds, quarantine cannot stop the import of products from the disease-producing country and prevent entry into the country.

  Unlike bacteria, pathogenic viruses do not have the ability to self-grow and infest animals such as humans and cells such as bacteria and propagate by utilizing the functions of the cells. Therefore, there are few effective antiviral drugs such as antibiotics against bacteria and the like, and it is effective to prevent infection with a disinfectant or the like.

  As such a disinfectant, a drug having a denaturing action on proteins constituting the virus is mainly used. The main mechanism of action of denaturation is by oxidation, by hydrolysis, by forming a salt with the viral coat protein, or by coagulating the viral coat protein. In general, disinfectants such as sodium hypochlorite solution, alkaline solution, formaldehyde solution and the like are used. A disinfection method using iodine has also been proposed (Japanese Patent Laid-Open No. 9-11615, Special Table 2000-516142).

  To prevent avian influenza infection, there are measures such as prevention of invasion of wild birds at poultry farms, early detection of virus entry by conducting screening, etc., use of inactivated vaccines, etc. Disinfection is highly effective.

JP-A-9-110615 Special table 2000-516142 JP 2007-314495 A JP-A-10-025205 JP-A-2005-179273

  Some of the above-mentioned disinfectants are known as disinfectants used against avian influenza, but disinfectants with excellent antibacterial effects that can be used safely for poultry such as poultry are desirable. It is.

In the disinfecting method of the present invention, the avian influenza virus is inactivated by bringing the disinfecting target into contact with a drug containing a liquid as an active ingredient to inactivate the avian influenza virus. As the soot, it is preferable to use a soot collection solution obtained from at least one woody material selected from hickory, oak, cherry, kunugi and oak.
The object to be disinfected is a poultry house, poultry hen or egg that may be contaminated with avian influenza virus. It is preferable to spray the poultry house and to spray and immerse the poultry chicken and egg.
The present invention relates to an avian influenza virus inactivating agent comprising phlegm as an active ingredient, and the phlegm solvent is preferably water, a water-soluble solvent or an oil-soluble solvent, and further preferably contains an antioxidant. .

  According to the present invention, the infectivity and toxicity of avian influenza virus can be effectively eliminated, and it is safe and easy to handle. Conventionally, it has not been known that an active ingredient derived from a natural product has an antibacterial effect against avian influenza, and it is not known that a liquid smoke for food has an inactivating action against avian influenza. In this way, since the liquid smoke is derived from natural products, there is very little burden on the global environment, and there is no fear of contaminating the environment when used for sterilization of chicken houses or food, and there are no concerns about safety and health for humans and animals.

  The active ingredient of the avian influenza virus inactivating agent of the present invention is a smoke (smoked flavor). The liquid smoke is obtained by collecting volatile components obtained by burning or dry-distilling (burning off air) wood, bamboo, sugarcane, corn and the like. In Japan, a law amending a part of the Food Sanitation Law and the Nutrition Improvement Law; In addition, the wood vinegar produced in the wood industry may be refined and processed into a sap. There are various methods for producing the liquid smoke that is the active ingredient of the present invention. Preferably, the dried wood material such as hickory having a predetermined size is burned in a furnace to obtain a solid product (charcoal, etc.). The liquid product is agglomerated (collected) by the condenser, excluding the gaseous product. Furthermore, a tar component etc. are removed in a refinement | purification process and a target object is obtained. There is also a method in which smoke obtained by combustion is absorbed in water and purified to make a soot.

  The wood material is preferably a soot collection liquid obtained from a wood material such as hickory, oak, cherry, kunugi, oak. Frozen liquid obtained by aggregation or collection with a solvent such as water is further diluted with various solvents such as water, water-soluble solvents such as ethanol or oily solvents such as vegetable oil, and is suitable for storage and transportation. Can be prepared in form. An antioxidant (such as ascorbic acid), an antiseptic, an emulsifier, a fragrance, and the like may be appropriately added to the agent of the present invention.

  The avian influenza virus inactivating agent of the present invention can be used as it is as a disinfectant for avian influenza viruses. The avian influenza virus in which the avian influenza virus inactivating agent of the present invention is effective is not particularly limited, but any one of HA (hemagglutinin) antigen subtypes 1 to 15 and NA (neuraminidase) antigen subtype 1 What has any 1 type of ~ 9 can be illustrated. In this case, there are 135 combinations in total, but when any one or more of them are included, the inactivation is excellent. At this time, the avian influenza virus is effective for at least one selected from the above combinations, and may include two or more other avian influenza viruses having different combinations. Thus, for example, when there is both an avian influenza virus of a combination of HA antigen subtype 1 and NA antigen subtype 2 and an avian influenza virus of a combination of HA antigen subtype 3 and NA antigen subtype 5 It may be. Examples of such avian influenza viruses include A / whistling swan / Shimane / 499/83 (H5N3), and the avian influenza viruses are Otsuki et al., Research in Veterinary Science, 1987, 43, pp. 177-179, Isolation of Influenza A viruses from migratory waterfowl in San-in District, Western Japan in the winter of 1983-1984; and Otsuki et al., Acta. Virol., 28: 524, 1987 "Isolation of H5 It is disclosed in Influenza viruses from Whistling Swans in Western Japan in November 1983.

  The avian influenza virus inactivating agent of the present invention can be used as it is, or is diluted 5 to 1,000 times, preferably 5 to 100 times with a solvent such as water at the time of use. Thus, the content of the carbonyl component which is a main component in the disinfectant diluted for use is finally adjusted to 0.05 to 40 g, preferably 0.5 to 40 g in 1 L.

Examples of the preferred liquid for the avian influenza virus inactivating agent of the present invention include AM-1, AM-2, and AM-3 manufactured by Mastertaste Inc. (Teterboro, NJ USA) manufactured by Hickory and Oak. These compositions are shown below.
AM-1
Phenol component (mg / ml) <0.25
Carbonyl component (mg / ml) 50-100
Acid component (acidity: acidity%) 0.3-2.3
AM-2
Phenol component (mg / ml) <1.7
Carbonyl component (mg / ml) 150-200
Acid component (acidity: acidity%) 3.5-5.6
AM-3
Phenolic component (mg / ml) 0.3-0.8
Carbonyl component (mg / ml) 150-200
Acid component (acidity: acidity%) 1.8-2.1

The preferred liquid smoke is the following general liquid smoke composition:
Phenol component (mg / ml) 7-43
Carbonyl component (mg / ml) 100-500
Acid component (acidity: acidity%) 10-17
There are few phenol components and acid components compared with.

As described above, the prepared avian influenza virus inactivating agent of the present invention is sprayed or applied to poultry houses, poultry chickens, and the like that may be contaminated with avian influenza virus with ordinary equipment and means, or disinfected in this prepared solution. Dip the target chickens, eggs and other foods into contact with drugs to disinfect them, and if necessary, wash them with clean water to inactivate the avian influenza virus.
By preparing the drug stock solution to be diluted at the time of use, transportation costs can be reduced. Any of the above solvents may be used as the solvent used for dilution during use.

  EXAMPLES Next, although an Example demonstrates this invention further more concretely, these do not restrict | limit this invention at all.

(Example)
The avian influenza virus inactivating agent of the present invention was prepared using three kinds of liquid smoke.

(Test material)
Liquid solution: AM-1, AM-2, AM-3 (manufactured by Mastertaste, Inc., USA)
Virus: Avian influenza virus A / whistling swan / Shimane / 499/83 (H5N3) strain
In 1983, Otsuka et al.
H5 subtype virus. This virus strain is strong by passing chicks.
Successfully poisoned.
Egg used: SPF 10-day-old chicken egg
Purchasing eggs from Aoki breeding ground in Tochigi prefecture, Kyoto Sangyo University bird flu
Incubated at the research center for experiments.

(Test method)
(1) PBS solution smoke was diluted to a predetermined ratio in (phosphate buffer solution) were prepared each 0.5 ml, 10-fold contrast, 10 was diluted with PBS to 100-fold ^9.5 EID / 0.2 ml of virus 0.5 ml of each liquid was mixed.
(2) It left still at room temperature for 10 minutes and 60 minutes, respectively.
(3) Immediately inoculate 0.2 ml of the test solution-virus mixture solution into the chorioallantoic cavity of three 10-day-old chicken eggs.
(4) The inoculated growing eggs were cultured at 37 ° C. for 48 hours, and then the presence or absence of virus growth in chorioallantoic fluid was confirmed by 0.5% chicken hemagglutination (HA) test. The maximum dilution ratio of the liquid in which no virus was detected in all three eggs was defined as the maximum effective dilution ratio of the liquid in each virus.

(Test results)

  As described above, the inactivating agent of the present invention was inactivated by contact with avian influenza virus at room temperature for 10 minutes, and no virus survival was observed at dilutions below the maximum effective dilution factor. The drug of the present invention has an excellent antiviral effect against avian influenza virus.

The present invention is an avian influenza virus inactivating agent that can be safely used for foods and is easy to handle, and is extremely effective for disinfecting poultry houses, poultry, chicken eggs, and other foods.

Claims (4)

A method for disinfecting an avian influenza virus, which inactivates the avian influenza virus by bringing a drug containing a liquid smoke into contact with an object to be disinfected.
The disinfecting method according to claim 1, wherein the smoke liquid is a smoke liquid collection liquid obtained from at least one woody material selected from hickory, oak, cherry, kunugi and oak.
The disinfection method according to claim 1, wherein the object to be disinfected is a poultry house, a poultry hen, or a chicken egg that may be contaminated with avian influenza virus.
The disinfection method according to claim 1, wherein the contact of the drug is spraying of the drug on the object to be disinfected or immersion in the drug.