CN111973582A - Application of n-caprylic acid or sodium n-caprylate in killing coronavirus - Google Patents
Application of n-caprylic acid or sodium n-caprylate in killing coronavirus Download PDFInfo
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- CN111973582A CN111973582A CN202010610797.2A CN202010610797A CN111973582A CN 111973582 A CN111973582 A CN 111973582A CN 202010610797 A CN202010610797 A CN 202010610797A CN 111973582 A CN111973582 A CN 111973582A
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- coronavirus
- sodium
- caprylate
- caprylic acid
- killing
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/19—Carboxylic acids, e.g. valproic acid
- A61K31/20—Carboxylic acids, e.g. valproic acid having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
Abstract
The invention provides an application of n-caprylic acid or sodium n-caprylate in killing coronavirus, which is specifically to prepare a disinfectant or a medicament for killing coronavirus by using the n-caprylic acid or the sodium n-caprylate. The invention can be used for preparing disinfectant, medicament and other products with virus killing effect by utilizing the virus killing effect of the n-caprylic acid and the sodium salt thereof. The n-octanoic acid and the sodium salt thereof have good effect of killing coronavirus, are easy to obtain, have low cost and have industrial application value.
Description
Technical Field
The invention belongs to the field of virus inactivation, and particularly relates to application of n-caprylic acid or sodium n-caprylate in killing coronavirus.
Background
Viruses of the genus coronaviruses are enveloped (envelope) RNA viruses with a linear single-stranded positive strand genome, and are a large group of viruses that are widely found in nature. Coronaviruses only infect vertebrates, such as humans, mice, pigs, cats, dogs, wolves, chickens, cows, birds. Coronaviruses can be divided into four genera according to phylogenetic trees: α, β, γ.
The alphacoronavirus includes human coronavirus 229E, human coronavirus NL63, Hemiphus rhamnus coronavirus HKU1, Hemiphus rhamnus coronavirus HKU8, Hemiphus rhamnus coronavirus HKU2, 8 kinds of porcine epidemic diarrhea virus PEDV, porcine transmissible gastroenteritis virus TGEV, canine coronavirus CCoV and feline coronavirus FCoV.
The beta genus coronavirus includes human coronavirus HKU1, murine coronavirus, Hepialus coronavirus HKU5, Hepialus coronavirus HKU9, 7 kinds of SARS-associated virus, bovine coronavirus BCoV, human coronavirus OC43, equine coronavirus ECoV porcine hemagglutinating encephalomyelitis virus PHEV, canine respiratory coronavirus CrCoV, etc.; the mouse coronavirus includes mouse hepatitis virus MHV, rat coronavirus and bird mouth sparrow virus. Severe Acute Respiratory Syndrome (SARS) -associated viruses include SARS virus and other similar SARS viruses.
The gamma-type coronavirus mainly comprises avian coronavirus such as avian infectious bronchitis virus IBV and beluga coronavirus SW 1. Avian coronaviruses include those causing infection of various birds such as chickens, turkeys, sparrows, ducks, geese, pigeons, the most predominant of which is avian infectious bronchitis virus.
The genus coronavirus includes oriole coronavirus BuCoV HKU11, thrum coronavirus ThCoV HKU12, bird coronavirus HKU12, Asian leopard cat coronavirus ALCCoV, Chinese ferret badger coronavirus CFBCoV, pig coronavirus PDCoV, meadow coronavirus WECoV, sparrow coronavirus SPCoV, magpie 40498coronavirus MRCoV, nocturn coronavirus NHCoV, wild duck coronavirus WiCoV, black water chicken coronavirus CMCoV, etc..
2019 the novel coronavirus 2019-nCoV, which can cause novel coronavirus pneumonia COVID-19, is the 7 th coronavirus which is known to infect people at present, and the other 6 coronavirus are HCoV-229E, HCoV-OC43, HCoV-NL63, HCoV-HKU1, SARS-CoV (causing severe acute respiratory syndrome) and MERS-CoV (causing middle east respiratory syndrome), respectively. As can be seen from the above, many animals carry coronavirus, and some coronavirus can cause large-area transmission after infecting human, resulting in serious epidemic situation, and preparation for coronavirus infecting human in terms of vaccine, medicine and the like is needed, so that an effective corresponding method can be provided when epidemic disease occurs.
Caprylic acid, also known as caprylic acid, is naturally found in nutmeg, lemon grass, apple, coconut oil, wine, hops, and the like, is an 8-carbon fatty acid, and is extracted from coconut oil by a volatile fatty acid fractionation method to obtain caprylic acid. N-octanoic acid (CAS)No.: 124-07-2), formula: c8H16O2Molecular weight: 144.21, it is colorless transparent oily liquid with sweat odor. The n-octanoic acid is mainly used for synthesizing essence and perfume and lubricating oil additive, and can also be used for preparing dye, medicine, plasticizer, lubricant, etc., and can also be used as antiseptic, bactericide and defoaming agent, etc. The solubility of the n-caprylic acid in water is low, but the sodium n-caprylate (sodium caprylate) obtained by saponification of the n-caprylic acid is easy to dissolve in water and convenient to use. Sodium n-octanoate (CAS No. 1984-06-1) with molecular formula C8H15NaO2And has a molecular weight of 166. The sodium caprylate can be used for protein protection and separation and precipitation in the pharmaceutical industry. In addition, sodium caprylate has an important role in eliminating residual viral contamination in blood products.
In recent years, enveloped viruses have caused great harm to humans, including this Covid-19 coronavirus, which causes new coronopneumonia, but no report has been made on the killing effect of n-octanoic acid or sodium octanoate on coronaviruses.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provides application of n-caprylic acid or sodium n-caprylate in killing coronavirus.
The technical scheme adopted by the invention is as follows:
an application of n-octanoic acid or sodium n-octanoate in killing coronavirus is disclosed.
Further, the application is specifically to the preparation of a disinfectant or a medicament for killing coronavirus by using n-caprylic acid or sodium n-caprylate.
Further, the concentration of the n-octanoic acid or the sodium n-octanoate in the disinfectant is 5-30 mM.
Furthermore, the pH value of the disinfectant solution of sodium n-caprylate is 3-7.
The invention has the beneficial effects that: the sodium n-caprylate can be used for preparing disinfectant, medicament and other products with virus killing effect by utilizing the virus killing effect of the sodium n-caprylate and the sodium salt thereof. The n-octanoic acid and the sodium salt thereof have good effect of killing coronavirus, are easy to obtain, have low cost and have industrial application value.
Detailed Description
The invention provides an application of n-caprylic acid or sodium n-caprylate in killing coronavirus, which is further explained by combining a specific embodiment.
EXAMPLE 1 examination of the Virus-killing Effect of sodium n-octanoate
The adopted cell is a human hepatoma cell line Huh7 which is purchased from Shanghai cell bank, and the virus strain is coronavirus 229E which is purchased from Hangzhou south immortal Biotechnology limited company.
The detection method comprises the following steps: sodium n-octanoate was added to make a 10mM solution, and the pH was adjusted to 5.0, at which time the solution turned into a uniform suspension. Mu.l of the above prepared sodium n-octanoate solution was added to each well of a 96-well plate, and 10. mu.l of coronavirus 229E (10 concentration) was added8CFU/ml). After leaving at room temperature for 1, 5, and 10 minutes, the cultures were taken out and placed on the cells, respectively, which were the test groups. A virus-free control group (containing sodium n-octanoate alone), a positive control group (containing coronavirus 229E without sodium n-octanoate), and a negative control group (PBS) were also prepared. Determining residual virus titer by cell pathology method, culturing at 37 deg.C in incubator for 5 days, determining result, and calculating LogTCID by Reed-Muench method50: wherein the distance ratio (percentage higher than 50% -50%)/(percentage higher than 50% -percentage lower than 50%), is added to the virus dilution higher than 50% of the lesions, i.e. the TCID of the virus50。
The results are shown in Table 1, which indicates that sodium n-octanoate has a good inactivation effect on coronavirus 229E.
TABLE 1 residual viral titer (LogTCID) of sodium n-octanoate inactivated coronavirus 229E50/0.1ml)
| Time of sterilization | 1 minute | 5 minutes | 10 minutes |
| Test set | ≤0.50 | ≤0.50 | ≤0.50 |
| Control group without virus | ≤0.50 | ≤0.50 | ≤0.50 |
| Positive control | 7.00 | 6.59 | 6.60 |
Example 2: the virus killing effect of n-octanoic acid
The adopted cell is a human hepatoma cell line Huh7, and the virus strain is coronavirus 229E.
The detection method comprises the following steps: taking the n-octanoic acid liquid, preparing the n-octanoic acid liquid into a 10mM solution with water, shaking and mixing uniformly to obtain a suspension. Mu.l of the above-prepared n-octanoic acid solution was added to each well of a 96-well plate, and 10. mu.l of coronavirus 229E was added thereto. After leaving at room temperature for 1, 5, and 10 minutes, the cultures were taken out and placed on the cells, respectively, which were the test groups. A virus-free control group (containing sodium n-octanoate alone), a positive control group (containing coronavirus 229E without sodium n-octanoate), and a negative control group (PBS) were also prepared. Determining residual virus titer by cell pathology method, culturing at 37 deg.C in incubator for 5 days, determining result, and calculating LogTCID by Reed-Muench method50: the results are shown in table 2, which indicates that caprylic acid has a good inactivation effect on coronavirus 229E.
TABLE 2 residual viral titer of caprylic acid inactivated coronavirus 229E (LogTCID50/0.1ml)
| Time of sterilization | 1 minute | 5 minutes | 10 minutes |
| Test set | ≤0.50 | ≤0.50 | ≤0.50 |
| Control group without virus | ≤0.50 | ≤0.50 | ≤0.50 |
| Positive control | 6.90 | 6.70 | 6.50 |
Example 3
The invention provides a coronavirus disinfection solution, which consists of n-caprylic acid or sodium n-caprylate, preferably, wherein the concentration of the n-caprylic acid or the sodium n-caprylate is 10 mM. The pH value of the sodium n-caprylate disinfectant is adjusted to 5.
In addition, other functional substances may also be combined, such as: the aromatic agent, the bactericide and the like are prepared into disinfectant for washing hands and disinfecting the environment, the ground and the surfaces of objects.
Claims (4)
1. An application of n-octanoic acid or sodium n-octanoate in killing coronavirus is disclosed.
2. The use according to claim 1, in particular for the preparation of a disinfectant or medicament for the killing of coronaviruses with n-octanoic acid or sodium n-octanoate.
3. Use according to claim 2, wherein the concentration of n-octanoic acid or sodium n-octanoate in the disinfecting liquid is 5-30 mM.
4. Use according to claim 3, wherein the sodium n-octanoate disinfectant has a pH of 3 to 7.
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| Application Number | Priority Date | Filing Date | Title |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112662731A (en) * | 2020-12-01 | 2021-04-16 | 杭州金域医学检验所有限公司 | Coronavirus sample preservation solution and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011161107A1 (en) * | 2010-06-22 | 2011-12-29 | Research Foundation For Medical Devices | Small-pool fractionation of immunoglobulin g using caprylic acid and disposable equipment |
| US20140163101A1 (en) * | 2012-08-09 | 2014-06-12 | Grifols, S.A. | Caprylate viral deactivation |
-
2020
- 2020-06-29 CN CN202010610797.2A patent/CN111973582A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011161107A1 (en) * | 2010-06-22 | 2011-12-29 | Research Foundation For Medical Devices | Small-pool fractionation of immunoglobulin g using caprylic acid and disposable equipment |
| US20140163101A1 (en) * | 2012-08-09 | 2014-06-12 | Grifols, S.A. | Caprylate viral deactivation |
Non-Patent Citations (1)
| Title |
|---|
| NICOLA F.FLETCHER等: "A novel antiviral formulation inhibits a range of enveloped viruses", 《BIORXIV》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112662731A (en) * | 2020-12-01 | 2021-04-16 | 杭州金域医学检验所有限公司 | Coronavirus sample preservation solution and application thereof |
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