JP5481118B2 - Skin external composition - Google Patents

Description

  The present invention relates to an external composition for skin.

  The dermis on the inner side of the skin is composed of fibroblasts and an extracellular matrix such as elastin and collagen produced outside the cells by the cells. And these maintain the structure of the dermis, and thus the skin is maintained in a state where elasticity, flexibility, and the like are maintained.

However, the production amount of extracellular matrix such as elastin and collagen is reduced or its degradation or denaturation is caused by ultraviolet irradiation or aging. As a result, the skin exhibits aging symptoms such as reduced elasticity and formation of wrinkles.
That is, skin aging symptoms caused by ultraviolet irradiation or aging are associated with a decrease in the production amount of the extracellular matrix.

  Therefore, in order to prevent skin aging symptoms, an external composition for skin that can promote the production of the extracellular matrix of the dermis, specifically, for example, an external composition for skin containing an extract from the flower part of a star fruit. It has been proposed (Patent Document 1).

  However, there is a problem that such an external composition for skin is not necessarily excellent in terms of the ability to promote the production of elastin among the extracellular matrix of the dermis.

JP 2006-8571 A

  This invention makes it a subject to provide the composition for external use of skin excellent in the production promotion performance of elastin in view of said problem.

As a result of intensive studies to solve the above problems, the present inventors have found that a composition containing a star fruit leaf extract and a maple extract has excellent elastin production promoting performance. The present invention has been completed.

The external composition for skin according to the present invention is characterized by containing an extract of a leaf of star fruit and an extract of maple.

Moreover, the weight ratio of the extract of the leaf of the star fruit and the maple extract is 8: 2 to 7: 3 or 3: 7 to 2: 8 in terms of dry matter . By a such weight ratio, ing to that elastin production-enhancing performance of the external composition for skin was better.
In addition, dry matter conversion means converting into the weight of the dry matter which is a residue remove | excluding the extraction solvent from the extract.

Since the composition for external use of the skin of the present invention contains an extract of a leaf of a star fruit and a maple extract, the composition has an excellent elastin production promoting effect.

The graph which shows the production amount of elastin. The graph which shows the production amount of elastin. The graph which shows the production amount of elastin.

Embodiments of the present invention will be described below.
The external composition for skin of this embodiment contains a star fruit extract and a maple extract. Moreover, the composition for external use of skin of this embodiment can contain components other than a star fruit extract and a maple extract, and usually contains water.

The above-mentioned star fruit extract is obtained by extracting a star fruit belonging to the genus Carambola (also referred to as a quince, scientific name: Averrhoa carambola L.) with an extraction solvent.
In addition, the star fruit extract means a star fruit extract with the extraction solvent, a diluted solution thereof, a concentrated solution thereof, or a dried product from which the extraction solvent has been removed.
When the said star fruit extract is the aspect of an extract, its dilution liquid, and its concentrate, the density | concentration is 0.1 to 10 weight% normally in conversion of a dry matter.

Examples of the portion of the star fruit extracted with the extraction solvent include fruits, flowers, leaves and the like, and leaves are used in that elastin production can be further promoted.

Examples of the extraction solvent include lower aliphatic alcohols having 1 to 5 carbon atoms such as ethanol and propanol, lower aliphatic ketones such as acetone and methyl ethyl ketone, and 2 to 5 carbon atoms such as 1,3-butylene glycol, glycerin, and propylene glycol. And polyhydric alcohols or water.
Examples of the extraction solvent include a mixture of two or more types of the extraction solvents. The mixing ratio is not particularly limited and can be adjusted as appropriate.

  Especially, as said extraction solvent, the extraction solvent containing water and ethanol is preferable, and the extraction solvent with which water and ethanol were mixed by the volume ratio of water: ethanol = 7: 3 to 2: 8 is more preferable.

  As the extraction method, a conventionally known general method can be employed. For example, a reflux method can be employed.

  Specifically, as the star fruit extract, for example, the leaf portion of the star fruit is dried or pulverized without being dried, and then extracted by refluxing with the extraction solvent using an extraction device such as a Soxhlet extractor. What is obtained is mentioned. Further, the amount of the extraction solvent is 5 to 15 times (weight ratio) of the extraction raw material (star fruit leaf), the extraction time is 1 to 3 hours, and the extraction temperature is 20 to 95 ° C. preferable.

  In addition, as said star fruit extract, what is marketed as raw materials, such as cosmetics, can be used, for example.

  The concentration of the star fruit extract in the external composition for skin is not particularly limited, but the concentration of the star fruit extract is preferably 0.00005 to 0.05% by weight in terms of dry matter. There exists an advantage that elastin production can be accelerated | stimulated because the density | concentration of a star fruit extract is 0.00005 weight% or more in dry matter conversion. Moreover, it is preferable that the density | concentration of a star fruit extract is 0.05 weight% or less in conversion of a dry matter from the point which can accelerate | stimulate elastin production with a smaller quantity.

The maple extract is obtained by extracting a plant belonging to the genus Acer with an extraction solvent.
As plants belonging to the maple genus, for example, Acer aidzuense (Karakogikaede), Acer amoenum (Oomomiji), Acer argutum (hemp leaves maple), Acer buergerianum (Acer Buergerianum), Acer campestre (Kobukaede), Acer carpinifolium (Acer Carpinifolium), Acer crataegifolium (Acer Crataegifolium), Acer diabolicum (Onimomiji), Acer distylum (Marubakaede), Acer ginnala (Korean Karakogikaede), Acer japonicum (Acer japonicum), Acer mono (Acer mono), Acer negundo (Acer negundo), Acer nigrum (black Maple ), Acer nikoense (Acer Maximowiczianum), Acer nipponicum (Acer Nipponicum), Acer palmatum (Acer palmatum), Acer platanoides (Europe maple), Acer pseudoplatanus (western Kaji maple), Acer pycnanthum (Hananoki), Acer rufinerve (Acer Rufinerve , Acer saccharum (Acer saccharum), Acer sieboldianum (Kohauchiwakaede), Acer tschonoskii (Minekaede), Acer ukurunduense (Acer caudatum), and the like.
The maple extract can also be obtained by extracting two or more kinds of plants belonging to the genus Maple with an extraction solvent.

The maple extract is preferably obtained by extracting Acer palmatum with an extraction solvent in that elastin production can be further promoted.

The maple extract means an extract of a plant belonging to the genus Maple using an extraction solvent, a diluted solution thereof, a concentrated solution thereof, or a dried product obtained by removing the extraction solvent.
When the maple extract is in the form of an extract, a diluted solution, and a concentrated solution, the concentration is usually 0.1 to 10% by weight in terms of dry matter.

  The part of the plant belonging to the genus Maple that is extracted with the extraction solvent is not particularly limited, and examples thereof include leaves, branches, roots, bark, and seeds. Especially, a leaf part is preferable at the point which can accelerate | stimulate elastin production more.

Examples of the extraction solvent include lower aliphatic alcohols having 1 to 5 carbon atoms such as ethanol and propanol, lower aliphatic ketones such as acetone and methyl ethyl ketone, and 2 to 5 carbon atoms such as 1,3-butylene glycol, glycerin, and propylene glycol. And polyhydric alcohols or water.
Examples of the extraction solvent include a mixture of two or more types of the extraction solvents. The mixing ratio is not particularly limited and can be adjusted as appropriate.

  Especially, as said extraction solvent, the extraction solvent containing water and ethanol is preferable, and the extraction solvent with which water and ethanol were mixed by the volume ratio of water: ethanol = 7: 3 to 2: 8 is more preferable.

  As the extraction method, a conventionally known general method can be employed. For example, a reflux method can be employed.

  Specifically, as the maple extract, for example, the leaf part of the maple leaf is dried or pulverized without drying, and then using an extraction device such as a Soxhlet extractor, water: ethanol = 7: 3 Those obtained by reflux extraction with a 2: 8 volume ratio extraction solvent are preferred. In addition, the amount of extraction solvent is 5 to 15 times (weight ratio) of the extraction raw material (the leaf portion of the maple), the extraction time is 1 to 3 hours, and the extraction temperature is 20 to 95 ° C. Is preferred.

  In addition, as said maple extract, what is marketed as raw materials, such as cosmetics, can be used, for example.

  The concentration of the maple extract in the external composition for skin is not particularly limited, but the concentration of the maple extract is preferably 0.00005 to 0.05% by weight in terms of dry matter. There exists an advantage that elastin production can be accelerated | stimulated because the density | concentration of a maple extract is 0.00005 weight% or more in dry matter conversion. Moreover, it is preferable that the density | concentration of a maple extract is 0.05 weight% or less in conversion of a dry matter from the point which can accelerate | stimulate elastin production with a smaller quantity.

  The combined concentration of the star fruit extract and the maple extract in the external composition for skin is not particularly limited, but the dried product of the star fruit extract and the maple extract is 0.0001 to 0.1. % By weight, more preferably 0.0002 to 0.01% by weight, still more preferably 0.0006 to 0.005% by weight, and 0.0012 to 0.0025% by weight. It is particularly preferred. When the total concentration of the star fruit extract and the maple extract is 0.0001% by weight or more in terms of dry matter, there is an advantage that elastin production can be further promoted. Moreover, it is preferable that this total density | concentration is 0.1 weight% or less in conversion of a dry matter from the point which can accelerate | stimulate elastin production with a smaller quantity.

  The content ratio of the star fruit extract and the maple extract in the external composition for skin is not particularly limited, but the weight ratio of the star fruit extract: maple extract = 8: 2-2 in terms of dry matter. : 8 is preferable, and a range of 7: 3 to 2: 8 is more preferable. When the weight ratio of the star fruit extract and the maple extract is in the range of 8: 2 to 2: 8 in terms of dry matter, there is an advantage that elastin production can be further promoted.

  Examples of the external composition for skin include an external composition for skin for pharmaceutical use and an external composition for skin for cosmetics.

  The external composition for skin can be prepared by a conventionally known general method.

In addition, the form of the external composition for skin of this invention is not limited, The various form employ | adopted in a general external composition for skin can be employ | adopted in the range which does not impair the effect of this invention.
For example, the composition for external use of the skin of the present invention can be in the form of gel, lotion, emulsion, cream, ointment, semisolid and the like. In addition, medicinal components, oils, dyes, preservatives, surfactants, fragrances, pigments and the like can be appropriately blended in the composition for external use of the present invention according to the dosage form.

  EXAMPLES Next, although an Example is given and this invention is demonstrated in more detail, this invention is not limited to these.

  The following test examples evaluate whether elastin production is promoted by starfruit extract or maple extract by measuring the amount of elastin produced during the culture of normal human skin fibroblasts (NHDF). did.

<Star fruit extract>
As the star fruit extract, the trade name “Star Fruit Leaf Extract BG30” (a leaf extract containing 1.0% by weight of a dry product excluding the extraction solvent manufactured by Maruzen Pharmaceutical Co., Ltd.) was used. Hereinafter, such an extract is referred to as “star fruit extract”.

<Maple extract>
As the maple extract, the trade name “Momiji Extract BG” (manufactured by Maruzen Pharmaceutical Co., Ltd., 1.0% by weight of the dried product excluding the extraction solvent, leaf extract) was used. Hereinafter, such an extract is referred to as “momiji extract”.

(Test Example 1)
For culture of normal human skin fibroblasts (NHDF), NHDF culture medium (DMEM) supplemented with 10% fetal bovine serum, 100 units penicillin, and 0.1 mg / mL streptomycin was used.
A 35 mm tissue culture dish (manufactured by IWAKI) was inoculated with 4 mL of NHDF suspended in DMEM so that the number of cells was 2 × 10 ⁶ (cell / ml). NHDF was cultured in an incubator (95 vol% air, 5 vol% carbon dioxide) at 37 ° C.
On the second day of the confluent culture, the medium until then was replaced with a DMEM (Doulbecco's modified Eagle's Medium) medium, and the star fruit extract was 0.25 wt% (0.0025 wt% in terms of dry matter). And further cultured for 24 hours.
Thereafter, 1 mL of a 250 mM oxalic acid solution was added to the medium to dissolve the details.
Subsequently, it was left at 100 ° C. for 60 minutes at room temperature, and the supernatant obtained by centrifugation was used as a cell lysate. The amount of elastin in the cell lysate was quantified using a Biocolor Fastin Elastin Assay kit.

(Test Example 2)
The amount of elastin was changed in the same manner as in Test Example 1 except that the maple extract was added to the medium so as to be 0.25 wt% (0.0025 wt% in terms of dry matter) instead of the star fruit extract. Quantified.

(Test Example 3)
Instead of the star fruit extract, a mixture of star fruit extract: maple extract = 7: 3 (weight ratio) is added to the medium so as to be 0.25 wt% (0.0025 wt% in terms of dry matter). Except for these points, the amount of elastin was quantified in the same manner as in Test Example 1.

(Test Example 4)
The amount of elastin was quantified in the same manner as in Test Example 1 except that nothing was added as the extract.

FIG. 1 shows the amount of elastin produced in Test Examples 1 to 4 as a relative value to the amount of elastin in Test Example 4 in which nothing was added as an extract.
As is clear from FIG. 1, it was found that elastin production was synergistically promoted by combining the star fruit extract and the maple extract.

(Test Example 5)
Test example, except that instead of the star fruit extract, a mixture of the star fruit extract and the maple extract was added to the medium so as to be 0.50% by weight (0.0050% by weight in terms of dry matter). The amount of elastin was quantified in the same manner as in 1.

(Test Example 6)
Test example, except that instead of the star fruit extract, a mixture of the star fruit extract and the maple extract was added to the medium so as to be 0.125% by weight (0.00125% by weight in terms of dry matter) The amount of elastin was quantified in the same manner as in 1.

(Test Example 7)
Test Example, except that instead of the star fruit extract, a mixture of the star fruit extract and the maple extract was added to the medium so as to be 0.0625 wt% (0.000625 wt% in terms of dry matter) The amount of elastin was quantified in the same manner as in 1.

  FIG. 2 shows the amount of elastin produced in Test Examples 3 to 7 as a relative value to the amount of elastin in Test Example 4 in which nothing was added as an extract.

(Test Example 8)
In place of the star fruit extract, a mixture of the star fruit extract and the maple extract was added to the medium so as to be 0.25 wt% (0.0025 wt% 8: 2 wt ratio in terms of dry matter). Except for the above, the amount of elastin was quantified in the same manner as in Test Example 1.

(Test Example 9)
In place of the star fruit extract, a mixture of the star fruit extract and the maple extract was added to the medium so as to be 0.25 wt% (0.0025 wt% 5: 5 wt ratio in terms of dry matter). Except for the above, the amount of elastin was quantified in the same manner as in Test Example 1.

(Test Example 10)
In place of the star fruit extract, a mixture of the star fruit extract and the maple extract was added to the medium so as to be 0.25 wt% (0.0025 wt% 3: 7 wt ratio in terms of dry matter). Except for the above, the amount of elastin was quantified in the same manner as in Test Example 1.

(Test Example 11)
In place of the star fruit extract, a mixture of the star fruit extract and maple extract was added to the medium so as to be 0.25% by weight (0.0025% by weight 2: 8% by weight in terms of dry matter). Except for the above, the amount of elastin was quantified in the same manner as in Test Example 1.

  FIG. 3 shows the amount of elastin produced in Test Examples 1 to 4 and 8 to 11 as a relative value to the amount of elastin in Test Example 4 in which nothing was added as an extract.

  The composition for external use of the skin of the present invention can be used, for example, in the form of emulsion, ointment, cream, gel, aerosol content, etc., and can be suitably used as a pharmaceutical, quasi-drug or cosmetic.

Claims (1)

Contains star fruit leaf extract and maple extract ,
The weight ratio of the star fruit leaf extract to the maple extract is a dry fruit equivalent of star fruit leaf extract: maple extract = 8: 2-7: 3, or 3: 7-2: 8. An external composition for skin which is 8.

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