JP5403593B2 - Automatic solution injection device - Google Patents
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Description
本発明は、微小なチップ上で化学分析を行う微小化学分析システムにおいて、自動的に溶液注入を行うデバイスに関する。 The present invention relates to a device that automatically injects a solution in a microchemical analysis system that performs chemical analysis on a microchip.
次世代の微小化学分析システム(μTAS)に求められる重要な機能の一つは自律的動作である。自律的動作を実現するに際し、送液制御は最も重要な課題である。あらかじめ微小なチップ上にプログラムが書きこまれ、外部から何も電気信号を与えなくても自発的に決められた手順にしたがって送液制御を行うことができれば、駆動装置側の負担が軽くなる。 One of the important functions required for the next generation microchemical analysis system (μTAS) is autonomous operation. In realizing autonomous operation, liquid feeding control is the most important issue. If a program is written in advance on a small chip and liquid feeding control can be performed in accordance with a procedure determined voluntarily without applying any electrical signal from the outside, the burden on the drive device side is reduced.
微小なチップにおいて、送液制御を実現する技術としては、μTASにおいて外部からの電気信号により駆動する圧電素子を用いたマイクロポンプにより送液制御を行う機構がある（例えば、特許文献１参照）。また、圧電素子を用いない手段としては、マイクロ流体デバイスにおいて、光応答性ガス発生樹脂に光を照射することにより発生するガスの圧力により駆動するマイクロポンプがある（例えば、特許文献２参照）。 As a technique for realizing liquid feeding control in a minute chip, there is a mechanism for performing liquid feeding control by a micro pump using a piezoelectric element driven by an external electric signal in μTAS (for example, see Patent Document 1). As a means not using a piezoelectric element, there is a micropump that is driven by the pressure of gas generated by irradiating light to a photoresponsive gas generating resin in a microfluidic device (see, for example, Patent Document 2).
しかしながら、特許文献１の技術においては、圧電素子を駆動するための電気信号をプログラムにしたがって与える必要がある。特許文献２の技術においても、光応答性ガス発生樹脂に光を照射する必要があるため、やはり、光源を駆動するための電気信号をプログラムにしたがって与える必要がある。したがって、特許文献１及び特許文献２の技術を用いてμTASの自律的動作を実現することはできない。 However, in the technique of Patent Document 1, it is necessary to supply an electric signal for driving the piezoelectric element according to a program. Also in the technique of Patent Document 2, since it is necessary to irradiate light to the photoresponsive gas generating resin, it is also necessary to provide an electric signal for driving the light source according to the program. Therefore, the autonomous operation of μTAS cannot be realized using the techniques of Patent Document 1 and Patent Document 2.
本発明は以上のような課題に鑑みてなされたものであり、その目的は、μTASの自律的動作を実現することにより、駆動装置側の負担を軽くすることを可能とした自動溶液注入デバイスを提供することにある。 The present invention has been made in view of the above problems, and an object of the present invention is to provide an automatic solution injection device that can reduce the burden on the drive device side by realizing autonomous operation of μTAS. It is to provide.
本発明の自動溶液注入デバイスは、被送液溶液を収容する被送液溶液注入区画と、被送液溶液注入区画との境界に位置するダイヤフラムと、ダイヤフラムにほぼ接する位置に固定された触媒物質とを有し、ガス発生溶液が外部から導入されるガス発生溶液注入区画とを有するマイクロポンプ部を備え、マイクロポンプ部は、ガス発生溶液注入区画に導入されたガス発生溶液が触媒物質に接触することにより生ずる気体が、ダイヤフラムを押圧して被送液溶液注入区画の容積を圧縮し、被送液溶液を押し出し送液を行い、ガス発生溶液注入区画は、ガス発生溶液送液用微小流路を介してガス発生溶液が導入されることを特徴とする。 The automatic solution injection device of the present invention includes a liquid solution injection section for storing a liquid supply solution, a diaphragm located at a boundary between the liquid supply solution injection section, and a catalyst substance fixed at a position substantially in contact with the diaphragm. And a micropump part having a gas generation solution injection section into which the gas generation solution is introduced from the outside. The micropump part is in contact with the catalyst substance by the gas generation solution introduced into the gas generation solution injection section. gas produced by the, presses the diaphragm to compress the volume of the liquid feed solution injection section, have rows feeding and extruding to be liquid feed solution, the gas generating solution injection compartment, gas generating solution feeding for small The gas generating solution is introduced through the flow path .
本発明の自動溶液注入デバイスによれば、微小流路内を進行する過酸化水素溶液の到着のタイミングにより自動的にポンプが作動するため、外部に回路やプログラムを用意する必要がない。 According to the automatic solution injection device of the present invention, the pump is automatically operated according to the arrival timing of the hydrogen peroxide solution traveling in the microchannel, so that it is not necessary to prepare a circuit or a program outside.
（第１実施形態）
図１は、本発明の第１実施形態の自動溶液注入デバイスの全体構成を示す図である。図１（ａ）は、本実施形態の自動溶液注入デバイスの正面図であり、図１（ｂ）は、本実施形態の自動溶液注入デバイスの側面図である。図１に示すように、本実施形態の自動溶液注入デバイス１は、PDMS基板２枚とガラス基板を積層して作製される。
(First embodiment)
FIG. 1 is a diagram showing an overall configuration of an automatic solution injection device according to a first embodiment of the present invention. FIG. 1A is a front view of the automatic solution injection device of the present embodiment, and FIG. 1B is a side view of the automatic solution injection device of the present embodiment. As shown in FIG. 1, the automatic solution injection device 1 of this embodiment is manufactured by laminating two PDMS substrates and a glass substrate.
図に示すように、本実施形態の自動溶液注入デバイス１は、ガラス基板１００と、第１のPoly(Dimethylsiloxane）(ＰＤＭＳ)基板２００と、ダイヤフラム３００と、第２のＰＤＭＳ基板４００と、アクリル板５００とがこの順番に積層して構成される。本実施形態の自動溶液注入デバイス１の概略の外形サイズは、10mm×14mm程度である。 As shown in the figure, the automatic solution injection device 1 of this embodiment includes a glass substrate 100, a first poly (dimethylsiloxane) (PDMS) substrate 200, a diaphragm 300, a second PDMS substrate 400, and an acrylic plate. 500 are stacked in this order. The approximate external size of the automatic solution injection device 1 of this embodiment is about 10 mm × 14 mm.
図２は、本実施形態の自動溶液注入デバイスの分解斜視図である。図３は、本実施形態の微小参照電極デバイス１における図１中のマイクロポンプ部Ａを拡大した図及びその概略断面図である。 FIG. 2 is an exploded perspective view of the automatic solution injection device of the present embodiment. FIG. 3 is an enlarged view of the micropump portion A in FIG. 1 in the micro reference electrode device 1 of the present embodiment and a schematic cross-sectional view thereof.
図２及び図３に示すように、本実施形態の自動溶液注入デバイスは、２枚のＰＤＭＳ基板のうち、上段には各種試薬溶液、下段には過酸化水素溶液を送液するための流路が形成される。それぞれの基板の流路にマイクロポンプ部が接続する。マイクロポンプ部は被送液溶液と二酸化マンガンを固定したゲルをダイヤフラムを介して配置することで構成される。 As shown in FIGS. 2 and 3, the automatic solution injection device of the present embodiment has a flow path for feeding various reagent solutions on the upper stage and a hydrogen peroxide solution on the lower stage among the two PDMS substrates. Is formed. A micropump unit is connected to the flow path of each substrate. The micropump unit is configured by arranging a gel to which a solution to be fed and manganese dioxide are fixed via a diaphragm.
本実施形態のガラス基板１００は平板状の部材であり、自動溶液注入デバイス１の底部の部材となる。 The glass substrate 100 of the present embodiment is a flat plate member and serves as a bottom member of the automatic solution injection device 1.
本実施形態の第１のＰＤＭＳ基板２００は、過酸化水素溶液注入区画２０１と、触媒固定ゲル２０２と、二酸化マンガン（触媒）粒子２０３と、過酸化水素溶液送液用微小流路２０４と、過酸化水素溶液供給区画２０５と、過酸化水素溶液送液用微小液絡路２０６と、空気抜き用流路２０７とを含んで構成される。 The first PDMS substrate 200 of this embodiment includes a hydrogen peroxide solution injection section 201, a catalyst fixing gel 202, manganese dioxide (catalyst) particles 203, a hydrogen peroxide solution feeding microchannel 204, It is configured to include a hydrogen oxide solution supply section 205, a hydrogen peroxide solution feeding micro liquid junction 206, and an air vent channel 207.
過酸化水素溶液注入区画２０１は、第１のＰＤＭＳ基板２００を貫通する円形断面の穴である。過酸化水素溶液注入区画２０１内のダイヤフラム３００側には、二酸化マンガン粒子２０３が触媒固定ゲル２０２により固定されている。また、過酸化水素溶液注入区画２０１内のガラス基板１００側に過酸化水素溶液が流入可能な所定の空間が確保できるように、触媒固定ゲル２０２が配置される。触媒固定ゲル２０２には、例えば、光硬化性のＰＶＡ-ＳｂＱゲルを用いることができる。 The hydrogen peroxide solution injection section 201 is a hole having a circular cross section that penetrates the first PDMS substrate 200. Manganese dioxide particles 203 are fixed by a catalyst fixing gel 202 on the diaphragm 300 side in the hydrogen peroxide solution injection section 201. Further, the catalyst fixing gel 202 is arranged so that a predetermined space into which the hydrogen peroxide solution can flow is secured on the glass substrate 100 side in the hydrogen peroxide solution injection section 201. As the catalyst fixing gel 202, for example, a photocurable PVA-SbQ gel can be used.
過酸化水素溶液送液用微小流路２０４は、過酸化水素溶液供給区画２０５と、過酸化水素溶液注入区画２０１とを接続する微小流路である。また、過酸化水素溶液送液用微小流路２０４は、過酸化水素溶液送液用微小液絡路２０６を介して過酸化水素溶液注入区画２０１に接続する。 The hydrogen peroxide solution feeding microchannel 204 is a microchannel that connects the hydrogen peroxide solution supply section 205 and the hydrogen peroxide solution injection section 201. The hydrogen peroxide solution feeding microchannel 204 is connected to the hydrogen peroxide solution injection section 201 via the hydrogen peroxide solution feeding microfluidic channel 206.
過酸化水素溶液送液用微小流路２０４の幅は、毛細管現象が発現する程度の幅であり、本実施形態では約５００μｍである。また、過酸化水素溶液送液用微小液絡路２０６の幅は、毛細管現象が発現する程度の幅であればよく、本実施形態では約３００μｍである The width of the micro-channel 204 for feeding a hydrogen peroxide solution is such a width that a capillary phenomenon appears, and is about 500 μm in this embodiment. Further, the width of the micro liquid junction 206 for feeding the hydrogen peroxide solution may be a width that allows the capillary phenomenon to appear, and is about 300 μm in this embodiment.
過酸化水素溶液供給区画２０５は、第１のＰＤＭＳ基板２００を貫通する円形断面の穴である。 The hydrogen peroxide solution supply section 205 is a hole having a circular cross section that penetrates the first PDMS substrate 200.
空気抜き用流路２０７は、過酸化水素溶液注入区画２０１から外部へ連絡する空気抜き穴である。過酸化水素溶液注入区画２０１内部の空気が空気抜き用流路２０７から排出されることにより、過酸化水素溶液は過酸化水素溶液注入区画２０１内に滑らかに導入される。 The air vent channel 207 is an air vent hole communicating from the hydrogen peroxide solution injection section 201 to the outside. By discharging the air inside the hydrogen peroxide solution injection section 201 from the air vent channel 207, the hydrogen peroxide solution is smoothly introduced into the hydrogen peroxide solution injection section 201.
本実施形態のダイヤフラム３００は、第１のＰＤＭＳ基板２００と第２のＰＤＭＳ基板４００の間に配置され、特に、第１のＰＤＭＳ基板２００の過酸化水素溶液注入区画２０１と、後述する第２のＰＤＭＳ基板４００の被送液溶液注入区画４０１との間に配置され、両区画を区分する。 The diaphragm 300 of the present embodiment is disposed between the first PDMS substrate 200 and the second PDMS substrate 400, and in particular, the hydrogen peroxide solution injection section 201 of the first PDMS substrate 200 and a second PD described later. It arrange | positions between the to-be-delivered solution injection | pouring sections 401 of PDMS substrate 400, and divides both sections.
本実施形態のダイヤフラム３００には、例えば、シリコーンフィルムを用いることができる。 For the diaphragm 300 of the present embodiment, for example, a silicone film can be used.
本実施形態の第２のＰＤＭＳ基板４００は、被送液溶液注入区画４０１と、被送液溶液用微小流路４０２と、注入口４０３と、被送液溶液用微小液絡路４０４とを含んで構成される。 The second PDMS substrate 400 of the present embodiment includes a liquid delivery solution injection section 401, a liquid feed solution microchannel 402, an inlet 403, and a liquid feed microfluidic channel 404. Consists of.
被送液溶液注入区画４０１は、第２のＰＤＭＳ基板４００を半貫通する円形断面の穴である。被送液溶液注入区画４０１は、ダイヤフラム３００に積層する側が開口となっており、アクリル板５００に接する側には注入口４０３が設けられる。 The liquid solution injection section 401 is a hole having a circular cross section that half-penetrates the second PDMS substrate 400. The liquid solution injection section 401 has an opening on the side laminated on the diaphragm 300, and an injection port 403 provided on the side in contact with the acrylic plate 500.
被送液溶液用微小流路４０２は、被送液溶液用微小液絡路４０４を介して被送液溶液注入区画４０１に接続し、また、図１に示すように、被送液溶液の吐出口４０２ａが自動溶液注入デバイス１の側面に形成される。 The liquid-sending solution micro-channel 402 is connected to the liquid-sending solution injection section 401 via the micro-liquid junction 404 for the liquid-sending solution, and, as shown in FIG. An outlet 402 a is formed on the side of the automatic solution injection device 1.
被送液溶液用微小流路４０２の幅は、約５００μｍである。また、被送液溶液用微小液絡路４０４の幅は、約３００μｍである。 The width of the microfluidic channel 402 for liquid to be delivered is about 500 μm. Further, the width of the micro liquid junction 404 for the solution to be delivered is about 300 μm.
本実施形態のアクリル板５００は、第２のＰＤＭＳ基板４００上に配置され、特に、第２のＰＤＭＳ基板４００の注入口４０３上部に配置される。 The acrylic plate 500 of the present embodiment is disposed on the second PDMS substrate 400, and in particular, is disposed on the inlet 403 of the second PDMS substrate 400.
ここで、図１のＡ部に示す、被送液溶液注入区画４０１、ダイヤフラム３００、過酸化水素溶液注入区画２０１、二酸化マンガン粒子２０３、及び、触媒固定ゲル２０２からなる箇所を、マイクロポンプ部Ａとする。 Here, the part consisting of the liquid solution injection section 401, the diaphragm 300, the hydrogen peroxide solution injection section 201, the manganese dioxide particles 203, and the catalyst fixing gel 202 shown in part A of FIG. And
次に、本実施形態のマイクロポンプ部の動作について説明をする。本実施形態のマイクロポンプ部の動作原理は非常にシンプルである。本実施形態の自動溶液注入デバイスは、過酸化水素から酸素気泡を生じる触媒反応を利用し、このとき過酸化水素溶液注入区画２０１に生じる体積変化をポンプ駆動力とするものである。 Next, the operation of the micropump unit of this embodiment will be described. The operating principle of the micropump unit of this embodiment is very simple. The automatic solution injection device of the present embodiment uses a catalytic reaction that generates oxygen bubbles from hydrogen peroxide, and the volume change generated in the hydrogen peroxide solution injection section 201 at this time is used as a pump driving force.
まず、被送液溶液注入区画４０１に被送液溶液を満たし、図３（ｂ）に示すようにアクリル板５００で封をする。次に、過酸化水素溶液供給区画２０５から過酸化水素溶液送液用微小流路２０４に過酸化水素溶液を導入すると、毛細管現象により溶液は流路中を自発的に送液され、過酸化水素溶液注入区画２０１内に浸入する。 First, the liquid-sending solution injection section 401 is filled with the liquid-sending solution and sealed with an acrylic plate 500 as shown in FIG. Next, when the hydrogen peroxide solution is introduced from the hydrogen peroxide solution supply section 205 into the hydrogen peroxide solution feeding micro-channel 204, the solution is spontaneously fed through the channel by capillary action, Enter the solution injection section 201.
図３（ｄ）に示すように、過酸化水素溶液が図中のＢ方向に送液され、過酸化水素溶液注入区画２０１内に過酸化水素溶液が供給されると、触媒固定ゲル２０２に固定された二酸化マンガン粒子２０３と過酸化水素溶液が接触し酸素が発生する。発生した酸素は泡（Bubbles）となり、ダイヤフラム３００を押し上げる。 As shown in FIG. 3D, when the hydrogen peroxide solution is fed in the direction B in the figure and the hydrogen peroxide solution is supplied into the hydrogen peroxide solution injection section 201, the hydrogen peroxide solution is fixed to the catalyst fixing gel 202. Oxygen is generated by contact between the manganese dioxide particles 203 and the hydrogen peroxide solution. The generated oxygen becomes bubbles and pushes up the diaphragm 300.
ダイヤフラム３００が押し上げられることにより、被送液溶液注入区画４０１の体積が減少し、被送液溶液注入区画４０１に注入された被送液溶液（Solution）は、図中のＡ方向に押し出され、被送液溶液用微小流路４０２に押し出される。 When the diaphragm 300 is pushed up, the volume of the liquid solution injection section 401 decreases, and the liquid solution (Solution) injected into the liquid solution injection section 401 is pushed out in the direction A in the figure, It is pushed out into the microfluidic channel 402 for the solution to be delivered.
（第２実施形態）
図４は、本発明の第２実施形態の自動溶液注入デバイスの全体構成を示す図である。図に示すように、本実施形態の自動溶液注入デバイスは、マイクロポンプを複数備える点で、第１実施形態の自動溶液注入デバイスとは相違がある。以下、第１実施形態の自動溶液注入デバイスと相違する構造部分について主に説明する。
(Second Embodiment)
FIG. 4 is a diagram showing the overall configuration of the automatic solution injection device according to the second embodiment of the present invention. As shown in the figure, the automatic solution injection device of this embodiment is different from the automatic solution injection device of the first embodiment in that it includes a plurality of micropumps. Hereinafter, the structure part different from the automatic solution injection device of the first embodiment will be mainly described.
図に示すように、複数のマイクロポンプＡ１〜Ａ３は過酸化水素溶液送液用微小流路２０４に各々結合している。図では、過酸化水素溶液供給区画２０５とマイクロポンプＡ１との距離をｌ1とし、過酸化水素溶液供給区画２０５とマイクロポンプＡ2との距離をｌ2とし、過酸化水素溶液供給区画２０５とマイクロポンプＡ3との距離をｌ3とする。 As shown in the drawing, the plurality of micropumps A 1 to A 3 are respectively coupled to the micro flow channel 204 for feeding a hydrogen peroxide solution. In the figure, the distance between the hydrogen peroxide solution feed compartment 205 and the micro pump A 1 and l 1, the distance between the hydrogen peroxide solution feed compartment 205 and the micro pump A 2 and l 2, the hydrogen peroxide solution feed compartment 205 And the distance between the micropump A 3 and l 3 .
そして、過酸化水素溶液供給区画２０５からの距離が短い順に各マイクロポンプＡ１〜Ａ３は動作し、各マイクロポンプＡ１〜Ａ３内の被送液溶液は、過酸化水素溶液の到達した順に、順次マイクロポンプＡ１〜Ａ３から排出される。 Then, the micro pump A 1 to A 3 distance to ascending order from the hydrogen peroxide solution supply section 205 operates, the liquid feed solution within the micro pump A 1 to A 3 has reached the hydrogen peroxide solution In order, the micro pumps A 1 to A 3 are sequentially discharged.
過酸化水素溶液送液用微小流路２０４とマイクロポンプＡ１〜Ａ３を適当な位置に配置することで、それぞれのマイクロポンプから被送液溶液が排出されるタイミングを自由にプログラムし制御できる。 By disposing the hydrogen peroxide solution feeding microchannel 204 and the micropumps A 1 to A 3 at appropriate positions, the timing at which the liquid feeding solution is discharged from each micropump can be freely programmed and controlled. .
また、マイクロポンプの駆動のタイミングは流路形状、ぬれ性や、流路の材質や、断面寸法により適宜制御することが可能である。 Further, the driving timing of the micropump can be appropriately controlled by the flow channel shape, wettability, flow channel material, and cross-sectional dimensions.
また、過酸化水素溶液送液用微小流路２０４および過酸化水素溶液注入区画２０１内への過酸化水素溶液の送液速度を調節するため、過酸化水素溶液には界面活性剤(Triton X-100)を添加してもよい。界面活性剤(Triton X-100)を添加することにより高速なスイッチングを行うことができる。 Further, in order to adjust the feeding speed of the hydrogen peroxide solution into the hydrogen peroxide solution feeding microchannel 204 and the hydrogen peroxide solution injecting section 201, a surfactant (Triton X- 100) may be added. High speed switching can be performed by adding a surfactant (Triton X-100).
このような方法により、マイクロポンプ駆動のタイミングはあらかじめプログラミング情報として自動溶液注入デバイス構造上に書き込むことが可能である。 By such a method, the timing of driving the micropump can be written in advance on the automatic solution injection device structure as programming information.
なお、本実施形態では複数のマイクロポンプＡ１〜Ａ３を直線状に配列したが、これに限られるものではなく、必要に応じて円弧状に配列してもよい。また、図４と同様の自動溶液注入デバイスを並列的に複数配置すれば、より複雑な溶液操作を行うこともできる。 In the present embodiment, the plurality of micropumps A 1 to A 3 are arranged in a straight line. However, the present invention is not limited to this, and may be arranged in an arc as necessary. If a plurality of automatic solution injection devices similar to those in FIG. 4 are arranged in parallel, a more complicated solution operation can be performed.
図５は、本発明の第２実施形態の自動溶液注入デバイスの実験結果を示す図である。 FIG. 5 is a diagram showing experimental results of the automatic solution injection device according to the second embodiment of the present invention.
プログラミング用流路に過酸化水素溶液を導入すると、溶液は自発的に流路中を進行し、各マイクロポンプ部の区画を満たした。そして、図５（ａ）に示すようにマイクロポンプ部では、ダイヤフラムの膨張と液溜め中の溶液の排出が認められた。プログラミング用流路の過酸化水素溶液は、一定時間後に次のポンプの区画を満たし、図５（ｂ）に示すように、同様の変化が認められた。 When the hydrogen peroxide solution was introduced into the programming channel, the solution spontaneously proceeded in the channel and filled each micropump section. And as shown to Fig.5 (a), expansion | swelling of the diaphragm and discharge | emission of the solution in a liquid reservoir were recognized in the micropump part. The hydrogen peroxide solution in the programming channel filled the next pump section after a certain time, and the same change was recognized as shown in FIG. 5 (b).
図５（ｃ）は、３つのポンプを並列的に並べ、それぞれのポンプから流路に順次溶液が排出される様子を示す。図５（ｄ）に示すように、溶液排出量が図中左側ほど大きいのは、過酸化水素溶液のポンプ部への到達時間の差を反映しており、位置関係で送液のタイミングをプログラミングできることが示された。なお、変化を起こすタイミングは流路の材質や断面寸法により調節できる。また、過酸化水素溶液中に界面活性剤を添加すると、送液速度が増大し、より高速なスイッチングを行うことができる。 FIG.5 (c) shows a mode that three pumps are arranged in parallel and a solution is discharged | emitted one by one to a flow path from each pump. As shown in FIG. 5 (d), the solution discharge amount is larger on the left side in the figure, reflecting the difference in the arrival time of the hydrogen peroxide solution to the pump unit, and the timing of liquid delivery is programmed according to the positional relationship. It was shown that it can be done. The timing at which the change occurs can be adjusted by the material of the flow path and the cross-sectional dimensions. In addition, when a surfactant is added to the hydrogen peroxide solution, the liquid feeding speed is increased, and higher speed switching can be performed.
（第３実施形態）
上記実施形態では、マイクロポンプで被送液溶液を押し出すことを行ったが、被送液溶液注入区画に被送液溶液を満たさず空気のみを満たしておき、他の流路内の溶液に圧力を及ぼすこともできる。図６はそのようなポンプを含むデバイスの例である。
(Third embodiment)
In the above embodiment, the liquid delivery solution is pushed out by the micropump, but the liquid delivery solution injection section is not filled with the liquid delivery solution but is filled only with air, and pressure is applied to the solution in the other channel. Can also be exerted. FIG. 6 is an example of a device including such a pump.
図６は、本発明の第３実施形態の自動溶液注入デバイスの全体構成を示す図である。図に示すように、本実施形態の自動溶液注入デバイスは、外側に空気を満たした補助マイクロポンプＢ１、Ｂ２を備えるとともに、内側に被送液溶液を満たしたマイクロポンプＡ１、Ａ２を備える点で、他の実施形態の自動溶液注入デバイスとは相違がある。以下、他の実施形態の自動溶液注入デバイスと相違する構造部分について主に説明する。 FIG. 6 is a diagram showing an overall configuration of an automatic solution injection device according to a third embodiment of the present invention. As shown in the figure, the automatic solution injection device of the present embodiment includes auxiliary micro pumps B 1 and B 2 filled with air on the outside, and micro pumps A 1 and A 2 filled with a solution to be delivered on the inside. Is different from the automatic solution injection device of other embodiments. Hereinafter, the structure part different from the automatic solution injection device of other embodiment is mainly demonstrated.
図６（ａ）は、本実施形態の自動溶液注入デバイスの正面図であり、図６（ｂ）は、本実施形態の自動溶液注入デバイスの側面図である。 FIG. 6A is a front view of the automatic solution injection device of the present embodiment, and FIG. 6B is a side view of the automatic solution injection device of the present embodiment.
図に示すように、本実施形態の自動溶液注入デバイス１は、ガラス基板１００と、第１のＰＤＭＳ基板２００と、ダイヤフラム３００と、第２のＰＤＭＳ基板４００と、アクリル板５００とがこの順番に積層して構成される。 As shown in the figure, in the automatic solution injection device 1 of this embodiment, the glass substrate 100, the first PDMS substrate 200, the diaphragm 300, the second PDMS substrate 400, and the acrylic plate 500 are arranged in this order. It is constructed by stacking.
図７（ａ）は、本実施形態の第２のＰＤＭＳ基板４００の正面図である。 FIG. 7A is a front view of the second PDMS substrate 400 of the present embodiment.
本実施形態の第２のＰＤＭＳ基板４００は、第１の被送液溶液注入区画４０１ａと、第２の被送液溶液注入区画４０１ｂと、被送液溶液合流用微小流路４０２と、第１の注入口４０３ａと、第２の注入口４０３ｂと、第１の被送液溶液用微小液絡路４０４ａと、第２の被送液溶液用微小液絡路４０４ｂと、第１の気室区画４０５ａと、第２の気室区画４０５ｂと、被送液溶液用微小流路４０６と、を含んで構成される。 The second PDMS substrate 400 of the present embodiment includes a first liquid delivery solution injection section 401a, a second liquid delivery solution injection section 401b, a liquid feed solution confluence microchannel 402, and a first liquid delivery solution injection section 401b. Injection port 403a, second injection port 403b, first liquid-feeding solution microfluidic channel 404a, second liquid-feeding solution microfluidic channel 404b, and first air chamber compartment 405a, a second air chamber section 405b, and a microfluidic channel 406 for liquid solution to be delivered.
第１の被送液溶液注入区画４０１ａ及び第２の被送液溶液注入区画４０１ｂは、第２のＰＤＭＳ基板４００を半貫通する円形断面の穴である。第１の被送液溶液注入区画４０１ａ及び第２の被送液溶液注入区画４０１ｂは、ダイヤフラム３００に積層する側が開口となっており、アクリル板５００に接する側には第１の注入口４０３ａ及び第２の注入口４０３ｂがそれぞれ設けられる。 The first liquid-feeding solution injection section 401 a and the second liquid-feeding solution injection section 401 b are circular cross-sectional holes that half-penetrate the second PDMS substrate 400. The first liquid delivery solution injection section 401 a and the second liquid delivery solution injection section 401 b have an opening on the side laminated on the diaphragm 300, and the first injection port 403 a and the side in contact with the acrylic plate 500. A second inlet 403b is provided.
被送液溶液合流用微小流路４０２は、第１の被送液溶液用微小液絡路４０４ａ及び第２の被送液溶液用微小液絡路４０４ｂを介して、各々第１の被送液溶液注入区画４０１ａ及び第２の被送液溶液注入区画４０１ｂに接続する。 Each of the microfluidic channels 402 for confluence of the liquid to be fed is provided with the first liquid to be fed via the first microfluidic channel 404a for the liquid to be fed and second microfluidic channel 404b for the liquid to be fed. It connects to the solution injection section 401a and the second liquid solution injection section 401b.
第１の気室区画４０５ａ及び第２の気室区画４０５ｂは、第２のＰＤＭＳ基板４００を半貫通する円形断面の穴であり、ダイヤフラム３００に積層する側が開口となっている。第１の気室区画４０５ａ及び第２の気室区画４０５ｂは、被送液溶液合流用微小流路４０２の両端部にそれぞれ接続する。 The first air chamber section 405a and the second air chamber section 405b are holes having a circular cross section that half-penetrates the second PDMS substrate 400, and the side stacked on the diaphragm 300 is an opening. The first air chamber compartment 405a and the second air chamber compartment 405b are respectively connected to both end portions of the microfluidic channel 402 for liquid solution merge.
被送液溶液用微小流路４０６の一端は、被送液溶液合流用微小流路４０２の、第１の被送液溶液用微小液絡路４０４ａ及び第２の被送液溶液用微小液絡路４０４ｂの接続箇所の中間に接続し、他端は自動溶液注入デバイス３の側面で終端をなす。 One end of the microfluidic channel 406 for the liquid solution to be fed is connected to the first microfluidic channel 404a for the liquid solution to be fed and the second microfluidic channel for the liquid solution to be fed. It connects to the middle of the connection part of the path 404b, and the other end terminates on the side surface of the automatic solution injection device 3.
図７（ｂ）は、本実施形態の第１のＰＤＭＳ基板２００の正面図である。 FIG. 7B is a front view of the first PDMS substrate 200 of the present embodiment.
本実施形態の第１のＰＤＭＳ基板２００は、第１の過酸化水素溶液注入区画２０１ａと、第２の過酸化水素溶液注入区画２０１ｂと、過酸化水素溶液送液用微小流路２０４と、過酸化水素溶液供給区画２０５と、第１の過酸化水素溶液送液用微小液絡路２０６ａと、第２の過酸化水素溶液送液用微小液絡路２０６ｂと、過酸化水素溶液用微小液絡路２０６ｃと、空気抜き用流路２０７ａ、２０７ｂと、第３の過酸化水素溶液注入区画２０８ａと、第４の過酸化水素溶液注入区画２０８ｂと、空気抜き用流路２０９ａ、２０９ｂとを含んで構成される。 The first PDMS substrate 200 of the present embodiment includes a first hydrogen peroxide solution injection section 201a, a second hydrogen peroxide solution injection section 201b, a hydrogen peroxide solution feeding microchannel 204, and an excess amount. Hydrogen oxide solution supply section 205, first hydrogen peroxide solution feeding microfluid 206a, second hydrogen peroxide solution feeding microfluid 206b, and hydrogen peroxide solution feeding microfluid It includes a passage 206c, air vent channels 207a and 207b, a third hydrogen peroxide solution injection section 208a, a fourth hydrogen peroxide solution injection section 208b, and air vent channels 209a and 209b. The
第１の過酸化水素溶液注入区画２０１ａ及び第２の過酸化水素溶液注入区画２０１ｂは、第１のＰＤＭＳ基板２００を貫通する円形断面の穴である。第１の過酸化水素溶液注入区画２０１ａと、第２の過酸化水素溶液注入区画２０１ｂ内のダイヤフラム３００側には、他の実施形態と同様に二酸化マンガン粒子が触媒固定ゲル（不図示）により固定されている。 The first hydrogen peroxide solution injection section 201 a and the second hydrogen peroxide solution injection section 201 b are circular cross-sectional holes that penetrate the first PDMS substrate 200. Similar to the other embodiments, manganese dioxide particles are fixed by a catalyst fixing gel (not shown) on the diaphragm 300 side in the first hydrogen peroxide solution injection section 201a and the second hydrogen peroxide solution injection section 201b. Has been.
過酸化水素溶液送液用微小流路２０４は、第１の過酸化水素溶液送液用微小液絡路２０６ａと、第２の過酸化水素溶液送液用微小液絡路２０６ｂをそれぞれ介して、第１の過酸化水素溶液注入区画２０１ａ及び第２の過酸化水素溶液注入区画２０１ｂに接続する。 The hydrogen peroxide solution feeding microfluidic channel 204 passes through the first hydrogen peroxide solution feeding microfluidic channel 206a and the second hydrogen peroxide solution feeding microfluidic channel 206b, respectively. The first hydrogen peroxide solution injection section 201a and the second hydrogen peroxide solution injection section 201b are connected.
過酸化水素溶液供給区画２０５は、第１のＰＤＭＳ基板２００を貫通する円形断面の穴である。過酸化水素溶液供給区画２０５は、過酸化水素溶液用微小液絡路２０６ｃを介して、過酸化水素溶液送液用微小流路２０４の、第１の過酸化水素溶液送液用微小液絡路２０６ａ及び第２の過酸化水素溶液送液用微小液絡路２０６ｂの接続箇所の中間に接続する。 The hydrogen peroxide solution supply section 205 is a hole having a circular cross section that penetrates the first PDMS substrate 200. The hydrogen peroxide solution supply section 205 is connected to the first hydrogen peroxide solution feeding microfluidic channel 204 of the hydrogen peroxide solution feeding microfluidic channel 204 via the hydrogen peroxide solution feeding microfluidic channel 206c. It connects in the middle of the connection location of 206a and the 2nd minute liquid junction 206b for hydrogen peroxide solution liquid feeding.
第３の過酸化水素溶液注入区画２０８ａ及び第４の過酸化水素溶液注入区画２０８ｂは、第１のＰＤＭＳ基板２００を貫通する円形断面の穴である。第３の過酸化水素溶液注入区画２０８ａ及び第４の過酸化水素溶液注入区画２０８ｂ内のダイヤフラム３００側には、第１の過酸化水素溶液注入区画２０１ａ及び第２の過酸化水素溶液注入区画２０１ｂと同様に二酸化マンガン粒子が触媒固定ゲル（不図示）により固定されている。 The third hydrogen peroxide solution injection section 208 a and the fourth hydrogen peroxide solution injection section 208 b are circular cross-sectional holes that penetrate the first PDMS substrate 200. In the third hydrogen peroxide solution injection section 208a and the fourth hydrogen peroxide solution injection section 208b, on the diaphragm 300 side, the first hydrogen peroxide solution injection section 201a and the second hydrogen peroxide solution injection section 201b are provided. Similarly, manganese dioxide particles are fixed by a catalyst fixing gel (not shown).
また、第３の過酸化水素溶液注入区画２０８ａ及び第４の過酸化水素溶液注入区画２０８ｂは、過酸化水素溶液送液用微小流路２０４の両端部にそれぞれ接続する。 Further, the third hydrogen peroxide solution injection section 208a and the fourth hydrogen peroxide solution injection section 208b are connected to both ends of the hydrogen peroxide solution feeding microchannel 204, respectively.
空気抜き用流路２０７ａ、２０７ｂ、２０９ａ、２０９ｂは、各過酸化水素溶液注入区画から外部へ連絡する空気抜き穴である。 The air vent flow paths 207a, 207b, 209a, and 209b are air vent holes communicating from the respective hydrogen peroxide solution injection sections to the outside.
本実施形態のダイヤフラム３００は、第１のＰＤＭＳ基板２００と第２のＰＤＭＳ基板４００の間に配置され、特に、第１の被送液溶液注入区画４０１ａ及び第１の過酸化水素溶液注入区画２０１ａとの間、第２の被送液溶液注入区画４０１ｂ及び第２の過酸化水素溶液注入区画２０１ｂとの間、第１の気室区画４０５ａ及び第３の過酸化水素溶液注入区画２０８ａとの間、および、第２の気室区画４０５ｂ及び第４の過酸化水素溶液注入区画２０８ｂとの間に配置され、両区画を区分する。 The diaphragm 300 of this embodiment is disposed between the first PDMS substrate 200 and the second PDMS substrate 400, and in particular, the first liquid solution injection section 401a and the first hydrogen peroxide solution injection section 201a. Between the second liquid solution injection section 401b and the second hydrogen peroxide solution injection section 201b, between the first air chamber section 405a and the third hydrogen peroxide solution injection section 208a. , And between the second air chamber compartment 405b and the fourth hydrogen peroxide solution injection compartment 208b, and separates both compartments.
ここで、図６のＡ１部に示す箇所をマイクロポンプ部Ａ１とし、Ａ２部に示す箇所をマイクロポンプ部Ａ２とし、Ｂ１部に示す箇所を補助マイクロポンプ部Ｂ１とし、Ｂ２部に示す箇所を補助マイクロポンプ部Ｂ２とする。 Here, the micropump unit A 1 the portions indicated in A part of FIG. 6, the portions indicated in 2 parts A and micropump unit A 2, the portions shown in part B and the auxiliary micropump unit B 1, B The part shown in part 2 is referred to as auxiliary micropump part B2.
次に、本実施形態のマイクロポンプ部の動作について説明をする。図８は、本発明の第３実施形態の自動溶液注入デバイスの実験結果を示す図である。 Next, the operation of the micropump unit of this embodiment will be described. FIG. 8 is a diagram showing experimental results of the automatic solution injection device according to the third embodiment of the present invention.
まず、過酸化水素溶液を過酸化水素溶液供給区画２０５に注入する。すると、過酸化水素溶液は、過酸化水素溶液送液用微小流路２０４を介して、第１の過酸化水素溶液注入区画２０１ａ及び第２の過酸化水素溶液注入区画２０１ｂに流入する（図８（ａ））。 First, the hydrogen peroxide solution is injected into the hydrogen peroxide solution supply section 205. Then, the hydrogen peroxide solution flows into the first hydrogen peroxide solution injection section 201a and the second hydrogen peroxide solution injection section 201b via the hydrogen peroxide solution feeding microchannel 204 (FIG. 8). (A)).
マイクロポンプＡ１及びＡ２が動作し、各マイクロポンプ内の被送液溶液が被送液溶液合流用微小流路４０２へ押し出される（図８（ｂ））。 Micropump A 1 and A 2 are operated, the liquid feed solution in each micropump is pushed into the liquid feed solution for confluence fine channel 402 (Figure 8 (b)).
次に、過酸化水素溶液は、過酸化水素溶液送液用微小流路２０４を介して、第３の過酸化水素溶液注入区画２０８ａ及び第４の過酸化水素溶液注入区画２０８ｂに流入する。すると、補助マイクロポンプＢ１及びＢ２が動作し、被送液溶液合流用微小流路４０２へ補助マイクロポンプＢ１及びＢ２内の空気を押し出す（図８（ｃ））。 Next, the hydrogen peroxide solution flows into the third hydrogen peroxide solution injection section 208a and the fourth hydrogen peroxide solution injection section 208b via the hydrogen peroxide solution feeding microchannel 204. Then, the auxiliary micropumps B 1 and B 2 operate to push out the air in the auxiliary micro pumps B 1 and B 2 to the microfluidic channel 402 for liquid solution merger (FIG. 8C).
被送液溶液合流用微小流路４０２へ押し出された被送液溶液は、補助マイクロポンプＢ１及びＢ２内から押し出された空気により、両端から加圧され、被送液溶液合流用微小流路４０２の中央部で２液の混合が促進され、混合液は、被送液溶液用微小流路４０６を介してデバイスの外部に吐出される（図８（ｄ））。 The liquid delivery solution pushed out to the liquid feed solution confluence microchannel 402 is pressurized from both ends by the air pushed out from the auxiliary micropumps B 1 and B 2 , and the liquid feed solution confluence microflow The mixing of the two liquids is promoted at the central portion of the path 402, and the mixed liquid is discharged to the outside of the device through the microfluidic channel 406 for liquid to be fed (FIG. 8D).
なお、上記各実施形態では、ガスを発生させる手段として、過酸化水素と二酸化マンガンを例としてあげたが、これに限られず、他の液体及び触媒の組み合わせも可能である。 In each of the above embodiments, hydrogen peroxide and manganese dioxide are taken as examples of means for generating gas, but the present invention is not limited to this, and other liquids and catalyst combinations are possible.
例えば、カタラーゼによる酵素反応では過酸化水素が基質となって酸素が発生する。したがって、二酸化マンガンの代わりにこれをゲル中に固定すれば、同様の動作が実現できる。また、炭酸水素イオンを含む溶液からは、低pHで二酸化炭素が発生する。したがって、ゲル２０１中に二酸化マンガン粒子２０３を固定せず、ここに塩酸等の酸をしみ込ませておき、過酸化水素の代わりに炭酸水素ナトリウム溶液を流し込み、過酸化水素と二酸化マンガンを用いた時と同様の変化を起こすことができる。同様の変化はアンモニウムイオンを含む溶液のpHを塩基溶液により上昇させることにより起こすこともできる。 For example, in an enzymatic reaction using catalase, oxygen is generated using hydrogen peroxide as a substrate. Therefore, if this is fixed in the gel instead of manganese dioxide, the same operation can be realized. In addition, carbon dioxide is generated at a low pH from a solution containing hydrogen carbonate ions. Therefore, when the manganese dioxide particles 203 are not fixed in the gel 201, an acid such as hydrochloric acid is soaked therein, a sodium hydrogen carbonate solution is poured instead of hydrogen peroxide, and hydrogen peroxide and manganese dioxide are used. Can cause similar changes. A similar change can be caused by raising the pH of a solution containing ammonium ions with a base solution.
さらに、酸化還元酵素による酵素反応では、過酸化水素が生成する。例えば、グルコースは酵素グルコースオキシダーゼにより酸化され、この際過酸化水素が生成する。この化学反応式は次の通りである。 Further, hydrogen peroxide is generated in the enzyme reaction by oxidoreductase. For example, glucose is oxidized by the enzyme glucose oxidase, producing hydrogen peroxide. This chemical reaction formula is as follows.
グルコース ＋ 酸素 → グルコノラクトン ＋ 過酸化水素 Glucose + oxygen → gluconolactone + hydrogen peroxide
したがって、ゲル２０１中に二酸化マンガン粒子２０３の代わりに酵素を固定し、過酸化水素の代わりにグルコース溶液を流せば、過酸化水素と二酸化マンガンを用いた時と同様の変化を起こすことができる。 Therefore, if an enzyme is immobilized in the gel 201 instead of the manganese dioxide particles 203 and a glucose solution is allowed to flow instead of hydrogen peroxide, the same change as when hydrogen peroxide and manganese dioxide are used can be caused.
以上説明したように、本発明の各実施形態による自動溶液注入デバイスによれば、微小流路内を進行する過酸化水素溶液の到着のタイミングにより順次自動的にポンプが作動するため、外部に回路やプログラムを用意する必要がない。 As described above, according to the automatic solution injection device according to each embodiment of the present invention, the pump automatically operates in sequence according to the arrival timing of the hydrogen peroxide solution that travels in the micro flow path. There is no need to prepare programs.
１：自動溶液注入デバイス
１００：ガラス基板
２００：第１のPoly(dimethylsiloxane）(ＰＤＭＳ)基板
２０１：過酸化水素溶液注入区画
２０２：触媒固定ゲル
２０３：二酸化マンガン（触媒）粒子
２０４：過酸化水素溶液送液用微小流路
２０５：過酸化水素溶液供給区画
２０６：過酸化水素溶液送液用微小液絡路
２０７：空気抜き用流路
３００：ダイヤフラム
４００：第２のＰＤＭＳ基板
４０１：被送液溶液注入区画
４０２：被送液溶液用微小流路
４０３：注入口
４０４：被送液溶液用微小液絡路
５００：アクリル板
1: Automatic solution injection device 100: Glass substrate 200: First Poly (dimethylsiloxane) (PDMS) substrate 201: Hydrogen peroxide solution injection section 202: Catalyst fixing gel 203: Manganese dioxide (catalyst) particles 204: Hydrogen peroxide solution Liquid flow microchannel 205: Hydrogen peroxide solution supply section 206: Hydrogen peroxide solution liquid microfluid 207: Air vent channel 300: Diaphragm 400: Second PDMS substrate 401: Liquid solution injection Section 402: Micro flow channel for liquid solution 403: Injection port 404: Micro liquid interface 500 for liquid solution 500: Acrylic plate
Claims (6)
前記被送液溶液注入区画の一面に備わるダイヤフラムと、
前記ダイヤフラムにほぼ接する位置に触媒物質が固定され、ガス発生溶液が導入されるガス発生溶液注入区画と、
を備えるマイクロポンプ部を少なくとも１つ有し、
前記マイクロポンプ部は、
前記ガス発生溶液注入区画に導入された前記ガス発生溶液が前記触媒物質に接触することにより生ずる気体が、前記ダイヤフラムを介して前記被送液溶液注入区画内の前記被送液溶液を押し出し送液を行い、
前記ガス発生溶液注入区画は、ガス発生溶液送液用微小流路を介して前記ガス発生溶液が導入されることを特徴とする自動溶液注入デバイス。 A liquid feeding solution injection section for containing the liquid feeding solution;
A diaphragm provided on one surface of the liquid solution injection section;
A gas generating solution injection section in which a catalytic material is fixed at a position substantially in contact with the diaphragm and a gas generating solution is introduced;
Having at least one micropump unit comprising:
The micropump part is
The gas generated when the gas generating solution introduced into the gas generating solution injection section comes into contact with the catalyst material pushes out the liquid supply solution in the liquid supply solution injection section through the diaphragm. the stomach line,
The automatic solution injection device, wherein the gas generation solution injection section is introduced with the gas generation solution via a gas generation solution feeding microchannel .
前記気室区画の一面に備わるダイヤフラムと、
前記ダイヤフラムにほぼ接する位置に触媒物質が固定され、ガス発生溶液が導入されるガス発生溶液注入区画と、
を備える補助マイクロポンプ部を少なくとも１つさらに有し、
前記補助マイクロポンプ部は、
前記ガス発生溶液注入区画に導入された前記ガス発生溶液が前記触媒物質に接触することにより生ずる気体が、前記ダイヤフラムを介して前記気室区画内の前記空気を押し出すことにより、前記被送液溶液に圧力を与えることを特徴とする請求項１に記載の自動溶液注入デバイス。 An air compartment containing air;
A diaphragm provided on one side of the air compartment;
A gas generating solution injection section in which a catalytic material is fixed at a position substantially in contact with the diaphragm and a gas generating solution is introduced;
Further comprising at least one auxiliary micropump unit comprising:
The auxiliary micropump part is
The liquid generated by the gas generated when the gas generating solution introduced into the gas generating solution injection section comes into contact with the catalyst material pushes out the air in the air chamber section through the diaphragm. The automatic solution injection device according to claim 1, wherein pressure is applied to the device.
二酸化マンガン及び過酸化水素溶液、酵素カタラーゼ及び過酸化水素溶液、酸及び炭酸水素イオンを含む溶液、塩基溶液及びアンモニウムイオンを含む溶液、酸化還元酵素及びその基質を含む溶液から選ばれた１つの組み合わせであることを特徴とする請求項１から３のいずれかに記載の自動溶液注入デバイス。 The catalyst material and the gas generating solution are:
One combination selected from manganese dioxide and hydrogen peroxide solution, enzyme catalase and hydrogen peroxide solution, solution containing acid and bicarbonate ions, solution containing base solution and ammonium ion, solution containing oxidoreductase and its substrate automatic solution injection device according to any of claims 1 to 3, characterized in that.
前記ガス発生溶液に界面活性剤を添加することにより送液速度を変化させて、送液のタイミングを調整することを特徴とする自動溶液注入デバイスの制御方法。
A control method for an automatic solution injection device according to claims 1 to 5,
A method for controlling an automatic solution injection device, wherein a liquid feeding speed is changed by adding a surfactant to the gas generating solution to adjust a liquid feeding timing.
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