JP4387123B2 - Sensing pump and sensor system using the same. - Google Patents

Description

[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a sensing pump that generates pressure by reacting with a specific substance. More specifically, the present invention relates to a pump that can be used for medical use such as a drug delivery system (DDS) by being driven according to a specific substance concentration in a living body.
[0002]
[Prior art]
Pumps that operate by converting energy into mechanical work have been used in a wide range of fields such as industrial machinery, waterworks and irrigation. For example, reciprocating pumps and rotary pumps convert electrical energy, pressure energy, etc. into mechanical work such as reciprocating motion or rotational motion. As an energy supply source of such a pump, a power source or a heating source that generates a vapor pressure is usually used.
[0003]
On the other hand, in the medical field, small analyzers and small medical instruments used for living bodies are actively developed. For example, for a drug delivery system, the pump as described above is also a component used to transport a fluid such as a drug. However, in addition to the pump, parts for controlling the supply amount, a power source, and the like are necessary. In addition, in order to supply a medicine or the like while reflecting diagnostic information, an analyzer or the like is necessary, and the system becomes complicated. In particular, when used in a living body, restrictions such as the supply of power and the size of the apparatus increase.
[0004]
[Problems to be solved by the invention]
Therefore, an object of the present invention is to provide a sensing pump capable of obtaining a mechanical driving force according to the concentration of a specific substance in the presence of the specific substance.
[0005]
[Means for Solving the Problems]
As a result of intensive studies, the present inventors have produced a pump that can obtain pressure energy corresponding to the concentration of a specific substance by utilizing a catalytic reaction accompanied by a volume change that reacts with a specific substance to generate a gas. The present inventors have found that this pump can achieve the above purpose.
[0006]
The present invention has been made based on the above findings, sensing, characterized in that a hollow body having an opening which is covered with a sensitive film that generates gas by reacting with a specific substance, and a pressure port A pump is provided.
The functional membrane is preferably a catalyst-immobilized membrane obtained by laminating a resin layer containing one or more kinds of catalysts on a dialysis membrane.
The catalyst-immobilized film may be formed by curing a water-soluble photosensitive resin containing one or more kinds of catalysts.
The catalyst is preferably a biocatalyst, and examples of the biocatalyst include enzymes and microorganisms. Examples of the enzyme include catalase, a mixture of catalase and glucose oxidase, and carbonic anhydrase.
The present invention also provides the sensing pump, a pressure gauge connected to the pressure port of the sensing pump, an A / D converter that converts an analog signal output from the pressure gauge into a digital signal, and the digital signal. A sensor system for detecting and quantifying a specific substance characterized by comprising a computer that processes and displays the result is provided.
[0007]
DETAILED DESCRIPTION OF THE INVENTION
Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings. In addition, the same code | symbol is attached | subjected to the same component and description is abbreviate | omitted.
FIG. 1 shows a configuration of a sensing pump according to an embodiment of the present invention.
Fig.1 (a) shows the structure of the sensing pump based on one Embodiment of this invention. As shown in FIG. 1A, the sensing pump according to the present embodiment includes a funnel-type hollow body 1, a sensitive film 2, and a ring-shaped retaining ring 3. The hollow body 1 has a pressure port 21 and an opening 22, the opening 22 is covered with a sensitive membrane 2, and the sensitive membrane 2 is fixed by a retaining ring 3 to construct a sensing pump. FIG.1 (b) is sectional drawing of the sensing pump constructed | assembled from the structure of Fig.1 (a).
[0008]
The sensing pump of the present invention obtains energy by the action that the sensitive membrane 2 reacts with a specific substance to generate a gas, and the generated gas increases the pressure inside the hollow body 1.
Examples of the sensitive membrane 2 used in the present invention include a catalyst-immobilized membrane in which a resin layer containing one or more kinds of catalysts is laminated on a dialysis membrane. Some catalytic reactions involve volume changes, and in particular, a catalyst that generates gas after the reaction by catalytic reaction can be used.
A biocatalyst is preferably used as the catalyst. Biocatalysts have high selectivity such as stereoselectivity, and the reaction conditions are normal temperature, normal pressure, and neutral, so no special environment is required and no load is applied to the surroundings. This is particularly advantageous when the sensing pump of the present invention is used in vivo. Specific examples include enzymes and microorganisms.
[0009]
Specific examples of the enzyme used in the present invention include catalase (EC 1.11.1.6). As shown in the following formula, catalase decomposes hydrogen peroxide into water and oxygen by catalytic reaction in the presence of hydrogen peroxide.
2H ₂ O ₂ → 2H ₂ O + O ₂
Catalase The oxygen generated in the above reaction can increase the pressure inside the hollow body 1 shown in FIG. A sensing pump using catalase as a catalyst is a pump for sensing hydrogen peroxide.
The catalase used in the present invention may be any as long as it can decompose hydrogen peroxide into water and oxygen by the above catalytic reaction. For example, natural products (human, other animal and plant biological samples, etc.) Produced from transformants obtained by transforming hosts such as Escherichia coli, yeast, animal cells, etc., which have been extracted, isolated, and produced from DNA, or obtained by genetic recombination and incorporating a gene encoding catalase. It may be isolated and purified.
[0010]
Specifically, the sensing pump shown in FIG. 1 is brought into contact with a liquid containing hydrogen peroxide so that at least the sensitive film 2 is immersed, and the hydrogen peroxide contained in the liquid in the container is sensitive. The catalase in the membrane reacts to generate oxygen, and the pressure inside the hollow body 1 increases.
[0011]
In the sensing pump of the present invention, the sensitive membrane 2 may be composed only of a membrane on which a catalyst is immobilized. For example, the sensitive membrane 2 may be constructed by laminating a layer on which a catalyst is immobilized on a dialysis membrane. FIG. 2 shows the structure of a sensitive membrane in which a layer in which a catalyst is immobilized is laminated on a dialysis membrane. In the sensitive membrane 2 shown in FIG. 2, a layer 5 in which a catalyst is immobilized is laminated on a dialysis membrane 4. By adopting such a configuration, the dialysis membrane 4 can prevent the catalyst from leaking out from the layer 5 on which the catalyst is immobilized. As a method for producing the sensitive film shown in FIG. 2, for example, a mixed solution of a catalyst and a photocrosslinkable resin is applied on the dialysis film, and then light irradiation is performed to cure the photocrosslinkable resin. The method of producing the sensitive film | membrane of this is mentioned. As the photocrosslinkable resin, water-soluble photosensitive resins such as stilbazolated polyvinyl alcohol (PVA-SbQ), polyvinyl cinnamate, and chitosan gel are preferable.
[0012]
In the present invention, the type of catalyst used may be one or more. For example, in the case of using two types of catalysts, the catalysts may be mixed and fixed in one layer, or a layer in which each catalyst is fixed may be laminated to form a two-layer structure. For example, when a mixture of catalase and glucose oxidase is used as a catalyst, a resin layer containing catalase and a resin layer containing glucose oxidase may be laminated on the dialysis membrane. Further, a resin layer containing a mixture of catalase and glucose oxidase may be laminated on the dialysis membrane.
[0013]
The shape of the hollow body used in the present invention is not particularly limited, and may be a funnel type as shown in FIG. 1 (a). Besides that, a cylindrical shape, a box shape, a conical shape, etc. It can be changed according to the application. However, in order to enhance the effect of increasing the pressure, it is preferable that the area of the opening covered with the sensitive film has a moderately large shape.
Moreover, the material of the hollow body may be an organic material, an inorganic material, or a composite material, and is not particularly limited. For example, it is preferable to select according to applications such as glass, metal, plastic, ceramics and the like. However, it is preferable that the material has a small volume change rate due to temperature or the like.
[0014]
The retaining ring is not particularly limited in its shape and material as long as it can fix the sensitive membrane so that no gap is generated in the opening of the hollow body. For example, a vinyl tube, a stopper using a spring, a silicone ring, a rubber ring, and the like can be given. Moreover, you may fix a sensitive film | membrane using an adhesive agent etc. instead of a retaining ring.
[0015]
The sensing pump of the present invention can obtain pressure energy according to the concentration of the specific substance by appropriately designing the sensitive membrane and the hollow body to be used.
[0016]
As a specific example, a hydrogen peroxide sensing pump using catalase will be described. As described above, the sensitive membrane can be prepared by applying a mixture of catalase and a photocrosslinkable resin on a dialysis membrane and drying it, followed by light irradiation to cure the photocrosslinkable resin. . The thickness of the dialysis membrane is not particularly limited, but is preferably in the range of 10 to 30 μm in consideration of permeability, strength and the like. Although there is no particular limitation on the pore size of the dialysis membrane, the range of ^{2 × 10 -3 ~5 × 10 -3} μm are preferred. The mixing ratio of the catalase and the photocrosslinkable resin is appropriately changed depending on the specific activity of the catalase used and the type of the photocrosslinkable resin. For example, when PVA-SbQ is used as the photocrosslinkable resin, the catalase is used. And the mixing ratio of PVA-SbQ are preferably in the range of 1:50 to 1: 1000 in terms of mass ratio. If the amount of catalase is small, the sensitivity of the sensitive membrane to hydrogen peroxide is too low, and a sufficient pressure increase may not be obtained. On the contrary, if the amount of catalase is too large, the mixture of the enzyme and the photocrosslinkable resin may be easily peeled off from the surface of the dialysis membrane.
[0017]
As the hollow body, for example, a funnel-type hollow body as shown in FIG. 1A can be used, and in the present invention, it is preferably made of glass. Shape and size of the opening to be covered by the sensitive film is not particularly limited, it is preferably the opening area in the range of 0.01～1000Cm ² against 4 cm ^2. Moreover, the shape and size of the pressure port are also changed according to the application. For example, when used for the application of generating a large pressure, a range of 0.1 to 10 m ² can be mentioned.
The inside of the hollow body is preferably filled with a buffer solution such as a phosphate buffer solution. In this case, the pH of the buffer solution is preferably close to the optimum pH of catalase.
[0018]
The sensing pump can obtain pressure energy at the pressure port by the reaction between hydrogen peroxide and catalase immobilized in the sensitive film in the presence of hydrogen peroxide. In addition, hydrogen peroxide can be quantified under conditions where the hydrogen peroxide concentration is proportional to the pressure increase rate.
[0019]
In the present invention, examples of using two types of catalysts include a sensing pump using catalase and glucose oxidase (EC 1.1.3.4).
[0020]
In this case, a sensitive membrane containing catalase and glucose oxidase can be used as the sensitive membrane of the sensing pump. Specifically, as a first catalyst, a solution obtained by mixing glucose oxidase, a photocrosslinkable resin, and a phosphate buffer solution is applied onto a dialysis membrane, dried, and then irradiated with light to thereby form the first catalyst. An immobilization layer is formed, and then, as a second catalyst, a buffer solution such as catalase, a photocrosslinkable resin, and a phosphate buffer (the pH of this buffer solution is close to the optimum pH of catalase and glucose oxidase) Is preferably applied) on the first catalyst immobilization layer formed earlier, dried and then subjected to light irradiation to form a second catalyst immobilization layer, A sensitive membrane having a configuration of dialysis membrane / first catalyst immobilization layer / second catalyst immobilization layer may be mentioned. In addition, as described above, it is not always necessary to form the second catalyst layer after forming the first catalyst-immobilized layer. Catalase and glucose oxidase are mixed to form the catalyst-immobilized layer. Also good.
[0021]
The sensing pump using the sensitive membrane can obtain pressure energy in the presence of glucose by a two-stage reaction as shown in the following formula.
Glucose + O ₂ → H ₂ O ₂ + Glucuronic acid glucose oxidase 2H ₂ O ₂ → H ₂ O + O ₂
Catalase [0022]
As a biocatalyst used when producing the sensing pump of the present invention, microorganisms can be used in addition to the enzymes described above. Examples of such microorganisms include yeasts such as yeast (Saccharomyces cerevisiae), bacteria such as staphylococci, Pseudomonas aeruginosa, Escherichia coli, Enterobacter, and Proteus.
A method for producing a sensing pump using microorganisms as a biocatalyst can also be carried out in the same manner as in the case of producing a sensing pump using an enzyme.
[0023]
When carbonic anhydrase is used as the enzyme, carbon dioxide can be generated by using hydrogen carbonate ions instead of hydrogen peroxide as described above.
[0024]
The sensing pump of the present invention can connect a component that converts generated pressure energy into mechanical work to the pressure port. This part can be selected as appropriate according to the work performed by the pump. For example, if it is a reciprocating motion, it can be a piston, plunger, diaphragm, etc., and if it is a rotational motion, it can be a gear, screw, vane. And the like having such functions. In addition, there is no particular limitation as long as it converts pressure energy into work.
[0025]
Since the sensing pump of the present invention can generate a pressure according to the concentration of a specific substance, it can be used for recognition and control of the specific substance. For example, when used as an actuator, a control operation such as opening and closing of a valve can be performed according to the concentration of a specific substance. Moreover, it can also be used as a sensor of a concentration measuring device by connecting a pressure gauge to the pressure port.
[0026]
In addition, since the sensing pump of the present invention can directly convert specific substances into kinetic energy without converting them into electricity or heat, there is a problem even when electricity cannot be supplied or heat generation becomes a problem. It can be used as a power source. In addition, since the mechanism for generating pressure energy is extremely simple, it can be applied to small analyzers (micro total analysis system, μTAS) and medical exercise bodies. For example, the present invention can be used for a moving body that directly drives a chemical substance (such as glucose) in a living body as an energy source, or a medical moving body that recognizes and drives a substance present in a diseased part (tumor).
[0027]
In addition, it can be used for chromatographic micro pumps, micro-dialysis micro pumps, and the like. Also, a chemical switch that can recognize chemical substances and press the mechanical switch according to the concentration; oxygen supply device for goldfish breeding tank, oxygen supply device that supplies oxygen gas during mountain climbing, exercise, etc .; fuel cell Fuel cell oxygen supply device for supplying high concentration oxygen to increase the output of the gas; an air display whose shape is deformed by increasing the volume used for the gas supply source of the art effect display by aeration such as liquid column, For example, grasping by artificial hand, curvature change in resinous hollow lens, air lift, press equipment, air bag; oxygen supply as energy used for lantern and figo; use for gas injection from outside in beverages, etc. it can.
[0028]
Next, the sensor system of the present invention will be described.
The sensor system of the present invention includes the above-described sensing pump of the present invention, a pressure gauge connected to the pressure port of the sensing pump, an A / D converter that converts an analog signal output from the pressure gauge into a digital signal, and And a computer for processing the digital signal and displaying the result.
Conventionally used pressure gauges, A / D converters, and computers can be used without any particular limitation. When catalase is used as the catalyst, hydrogen peroxide can be detected and quantified by the sensor system of the present invention. Further, when a mixture of catalase and glucose oxidase is used as a catalyst, glucose can be detected and quantified by the sensor system of the present invention.
[0029]
【Example】
Hereinafter, the present invention will be described in more detail with reference to examples. Needless to say, the scope of the present invention is not limited to such examples.
[0030]
Example 1
A sensing pump shown in FIG. 1 was produced. As shown in FIG. 1A, the sensing pump includes a funnel-type glass hollow body having a pressure port (upper end port) having an inner diameter of 7 mm and an opening portion (lower end port) having an inner diameter of 23 mm, a sensitive film, and vinyl. It consisted of a tube.
The sensitive film was produced by the following method. Catalase (EC 1.11.1.6, specific activity: 65000 U / mg) was used as a catalyst, and this catalase and a photocrosslinkable resin, stilbazolated polyvinyl alcohol (PVA-SbQ), were mixed at a mass ratio of 1: 1. The solution was applied onto a dialysis membrane (film thickness: 15 μm, pore size: 3 × 10 ⁻³ μm) and dried in a dark room for 1 hour. Then, the catalyst fixed layer was formed by performing fluorescence irradiation for 2 hours, and the sensitive film | membrane which consists of a dialysis membrane and a catalyst fixed layer was produced. The film thickness of the catalyst immobilization layer was 3.0 μm. The sensitive membrane was fixed to the opening of the hollow body with a vinyl tube so that the catalyst immobilization layer was on the hollow body side, and a sensing pump was produced.
[0031]
An experimental apparatus as shown in FIG. 3 was assembled using this sensing pump. After adding 6 ml of phosphate buffer (pH 7.0) to the inside of the sensing pump, a capacitive pressure gauge 6 (manufactured by Toyota Koki Co., Ltd., trade name “capacitance type pressure sensor” is connected to the pressure port of the sensing pump. Module ”), an A / D converter 7 for converting an analog signal output from the pressure gauge 6 into a digital signal, and a computer 8 for processing the digital signal and displaying the result. Furthermore, a power source 9 was connected to the pressure gauge 6. The sensing pump was installed so that the sensitive film was immersed in the sample 11 in the beaker 10.
[0032]
The sample was prepared by dropping 0.5 ml of hydrogen peroxide into 18 ml of phosphate buffer (pH 7.0) and gently stirring. Here, the concentration of hydrogen peroxide in the sample was changed by changing the concentration of hydrogen peroxide to be dropped. Using a series of samples having different hydrogen peroxide concentrations, the pressure generated inside the sensing pump was measured for a certain period of time with the time when hydrogen peroxide was dropped being set to time zero. The pressure increase rate (Pa / sec) at each hydrogen peroxide concentration was determined from the obtained time-pressure data. FIG. 4 is a graph showing the results of obtaining the pressure increase rate. FIG. 4A is a graph showing the relationship between time and pressure increase rate at various hydrogen peroxide concentrations. As shown in FIG. 4 (a), it was found that the pressure increase rate increased with time and stabilized in about 8 minutes. Next, FIG. 4B shows a graph showing the relationship between the pressure increase rate after 8 minutes from the dropping of hydrogen peroxide and the hydrogen peroxide concentration.
[0033]
As shown in FIG. 4B, an increase in pressure inside the sensing pump was confirmed when the hydrogen peroxide concentration in the sample was around 10 mmol / L, and the pressure inside the sensing pump further increased as the concentration of hydrogen peroxide increased. The increase rate was also increased, and it was found that a pressure increase rate of 1.0 Pa / sec was obtained when the hydrogen peroxide concentration was around 30 mmol / L. In addition, when the obtained work amount was calculated under the condition of the flow rate characteristic (hydrogen peroxide concentration: 120 mmol / l) by this system, it was 4 × 10 ⁻⁷ watts. These confirmed that the sensing pump was a source of pressure energy in the presence of hydrogen peroxide.
In addition, when the hydrogen peroxide concentration is in the range of 28.73 to 54.58 mmol / L, a high correlation is obtained between the hydrogen peroxide concentration and the pressure increase rate, and the sensing pump can be used for the determination of hydrogen peroxide. Was also confirmed.
[0034]
(Example 2)
In Example 1, the experimental apparatus was assembled in the same manner as in Example 1 except that the sensitive film of the sensing pump was changed to the sensitive film shown below.
Using glucose oxidase (EC 1.1.3.4, specific activity: 100 U / mg) as the first catalyst, this glucose oxidase, a photocrosslinkable resin, stilbazolated polyvinyl alcohol (PVA-SbQ), and a phosphate buffer A solution prepared by mixing (pH 7.0) with a mass ratio of 3: 100: 50 was applied onto a dialysis membrane (film thickness: 15 μm, pore size: 3 × 10 ⁻³ μm) and dried in a dark room for 1 hour.
[0035]
Next, the first catalyst-immobilized layer was formed by performing fluorescence irradiation for 2 hours, and further dried in a dark room for 7 hours. Next, catalase (EC 1.11.1.6, specific activity: 11400 U / mg) was used as the second catalyst. This catalase, a photolabile resin, stilbazolated polyvinyl alcohol (PVA-SbQ), and phosphate buffer were used. A liquid obtained by mixing the liquid (pH 7.0) at a mass ratio of 2:30:15 was applied onto the previously formed first catalyst immobilization layer and dried in a dark room for 1 hour. Subsequently, the 2nd catalyst fixed layer was formed by performing fluorescence irradiation for 2 hours, and the sensitive film | membrane which has a structure of a dialysis membrane / first catalyst fixed layer / second catalyst fixed layer was produced.
[0036]
A sample was prepared by adding 0.5 ml of a glucose solution (1000 mmol / L) dropwise to 18 ml of phosphate buffer (pH 7.0) and gently stirring the sample. Using this sample, the pressure generated inside the sensing pump was measured for a certain period of time with the time of dropping of glucose as time 0, and a pressure increase of 40 Pa was confirmed in 20 minutes.
[0037]
(Example 3)
In Example 1, an experimental apparatus was assembled in the same manner as in Example 1 except that the sensitive film of the sensing pump was changed to the sensitive film shown below.
Yeast Saccharomyces cerevisiae was used as a catalyst. Saccharomyces cerevisiae was cultured in YPD medium (1000 ml water, 10 g yeast extract, 20 g polypeptone, 20 g glucose, 20 g agar). For yeast culture, yeast cultured in a liquid medium was inoculated into the YPD medium medium, cultured at 30 ° C. for 24 hours, collected, and used for the production of a sensitive membrane.
A solution prepared by mixing yeast colony, phosphate buffer (pH 7.0), and photocrosslinkable resin (PVA-SbQ) at a mass ratio of 1:25:50 is a dialysis membrane (film thickness: 15 μm, pore size: 3 × 10 ^-3 μm), and dried in a dark room for 1 hour, followed by photocrosslinking by irradiation with a fluorescent lamp for 2 hours and immobilization to prepare a sensitive film. A sensing pump was produced in the same manner as in Example 1 using the obtained sensitive film.
[0038]
The sample was prepared by dropping 0.5 ml of hydrogen peroxide into 18 ml of phosphate buffer (pH 7.0) and gently stirring. Here, the concentration of hydrogen peroxide in the sample was changed by changing the concentration of hydrogen peroxide to be dropped. Using a series of samples having different hydrogen peroxide concentrations, the pressure generated inside the sensing pump was measured for a certain period of time with the time when hydrogen peroxide was dropped being set to time zero. The pressure increase rate (Pa / sec) at each hydrogen peroxide concentration was determined from the obtained time-pressure data. FIG. 5 is a graph showing the results of determining the pressure increase rate. FIG. 5 (a) is a graph showing the relationship between time and pressure increase rate at various hydrogen peroxide concentrations. As shown in FIG. 5 (a), it was found that the pressure increase rate increased with time. Next, FIG. 5B shows a graph showing the relationship between the pressure increase rate after 8 minutes from the dropping of hydrogen peroxide and the hydrogen peroxide concentration.
[0039]
As shown in FIG. 5B, an increase in pressure inside the sensing pump was confirmed when the hydrogen peroxide concentration in the sample was around 50 mmol / L, and the pressure inside the sensing pump further increased as the concentration of hydrogen peroxide increased. The increase rate was also increased, and it was found that a pressure increase rate of 0.3 Pa / sec was obtained when the hydrogen peroxide concentration was around 100 mmol / L. Moreover, when the hydrogen peroxide concentration was in the range of 50 to 220 mmol / L, a high correlation was obtained between the hydrogen peroxide concentration and the pressure increase rate, and it was confirmed that the sensing pump could be used for the determination of hydrogen peroxide. .
[0040]
【The invention's effect】
As described above in detail, according to the present invention, it is possible to provide a sensing pump capable of obtaining a mechanical driving force in accordance with the concentration of a specific substance in the presence of the specific substance.
[Brief description of the drawings]
1A and 1B are diagrams for explaining a sensing pump according to an embodiment of the present invention, FIG. 1A is a perspective view showing a configuration of the sensing pump, and FIG. 1B is a cross-sectional view of the sensing pump.
FIG. 2 is a diagram showing a layer structure of a sensitive film used in the sensing pump of the present invention.
FIG. 3 is a configuration diagram of an experimental apparatus using the sensing pump of the example.
FIG. 4 is a graph showing the pressure increase rate inside the sensing pump with respect to the hydrogen peroxide concentration.
FIG. 5 is a graph showing the pressure increase rate inside the sensing pump with respect to the hydrogen peroxide concentration.
[Explanation of symbols]
A Sensing pump 1 Hollow body 2 Sensitive membrane 3 Retaining ring 4 Dialysis membrane 5 Catalyst-immobilized layer 6 Pressure gauge 7 A / D converter 8 Computer 9 Power supply 10 Beaker 11 Sample

Claims (12)

A sensing pump capable of obtaining pressure energy corresponding to the concentration of a specific substance in the presence of the specific substance,
An opening covered with a sensitive membrane that reacts with a specific substance to generate a gas;
Ri Do hollow body and a pressure port,
The sensitive membrane is formed by laminating a resin layer containing one or more catalysts that generate a gas after the reaction on the membrane so that the resin layer is on the hollow body side and prevents the catalyst from leaking outside. sensing pump, wherein Rukoto such by. The resin layer is one formed by curing a water-soluble photosensitive resin containing one or more catalysts, sensing pump according to claim 1. The sensing pump according to claim 1 or 2, wherein the catalyst is a biocatalyst . The sensing pump according to claim 1 or 2 , wherein the catalyst is an enzyme . The sensing pump according to claim 1 or 2 , wherein the catalyst is catalase . Wherein said catalyst is a catalase, a mixture of glucose oxidase, sensing pump according to claim 1 or 2. The resin layer is a resin layer containing a catalase, ing by laminating a resin layer containing a glucose oxidase, sensing pump according to claim 1. The sensing pump according to claim 1 or 2 , wherein the catalyst contains a microorganism . The sensing pump according to claim 8, wherein the microorganism is yeast . The sensing pump according to any one of claims 1 to 9,
A pressure gauge connected to the pressure port of the sensing pump;
An A / D converter that converts an analog signal output from the pressure gauge into a digital signal;
A sensor system for detecting and quantifying a specific substance, comprising a computer that processes the digital signal and displays the result . A sensing pump according to claim 4 ;
A pressure gauge connected to the pressure port of the sensing pump;
An A / D converter that converts an analog signal output from the pressure gauge into a digital signal;
A sensor system for detecting and quantifying hydrogen peroxide , comprising a computer that processes the digital signal and displays the result. A sensing pump according to claim 6 or 7 ,
A pressure gauge connected to the pressure port of the sensing pump;
An A / D converter that converts an analog signal output from the pressure gauge into a digital signal;
A sensor system for detecting and quantifying glucose , comprising a computer for processing the digital signal and displaying the result.

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