JP5391536B2 - Seaweed-derived product material enriched with physiologically active substances, production method thereof, and seaweed-derived product - Google Patents

Description

  The present invention relates to a novel seaweed-derived product material and a production method in which physiologically active ingredients are concentrated.

It is known that the fat-soluble component contained in seaweed exhibits various physiological activities.
For example, in the case of wakame, which is a brown algae, its fat-soluble components include carotenoids such as fucoxanthin and β-carotene, sterols such as fucosterol and 24-methylenecholesterol, octadecatetraenoic acid and eicosapentaenoic acid, etc. Fatty acids are included.
With respect to carotenoids including fucoxanthin and β-carotene, various physiological activities such as antioxidant action and anticancer action have been reported (Non-patent Document 1). In particular, for fucoxanthin, anti-obesity effect (Non-patent document 2), high apoptosis-inducing ability to cancer cells (Non-patent document 3), blood glucose level increase inhibiting action (unpublished data), DHA synthesis promoting action (Non-patent document 4) ).
Regarding fucosterol, an inhibitory effect on cholesterol absorption has been reported (Non-Patent Document 5). Regarding eicosapentaenoic acid, various physiological activities such as blood lipid lowering action, blood pressure lowering action, antithrombotic action, anti-inflammatory action, and anticancer action have been reported (Non-patent Document 6).

  As described above, since fat-soluble components contained in seaweed, particularly carotenoids, exhibit useful physiological activity, various fat-soluble components themselves and / or methods for producing seaweeds enriched with fat-soluble components have been disclosed so far. . These manufacturing methods can be roughly divided into two manufacturing methods. One is a method for producing a fat-soluble oil (1) obtained by bringing seaweed into contact with an organic solvent and extracting the fat-soluble component in the organic solvent; It is the seaweed which concentrated the fat-soluble component which removed the component, and the manufacturing method (2) of the fat-soluble oil extracted from this seaweed with the organic solvent.

  Regarding the production method (1), Patent Document 1, Patent Document 2, Patent Document 3, Patent Document 4, Patent Document 5, Patent Document 6, Patent Document 7, Patent Document 8, Patent Document 9, Patent Document 10, Patent Document 11 etc. are disclosed. The seaweed-derived fat-soluble oil obtained by these production methods has a high concentration of the fat-soluble oil itself, but uses an organic solvent, so that the practical application is limited.

In Japan, in accordance with Articles 6 and 7 of the Food Sanitation Law, the extraction solvents that can be used in the food production process are ethanol and water, and those obtained using other solvents fall under the category of additives. To do. The use of solvents other than ethanol and water is prohibited in principle unless otherwise specified by the Minister of Health and Welfare. For example, hexane is recognized as a processing aid when extracting fats and oils from soybeans and the like, but is not currently recognized as an extraction solvent for fat-soluble components from seaweed. Therefore, the method for producing a seaweed-derived fat-soluble oil according to the above method has a drawback that it cannot be used for food applications.
Moreover, the stability of the extracted seaweed-derived fat-soluble oil is very poor and is not suitable for long-term storage. In addition, extraction of fat-soluble components requires a large amount of organic solvent, extraction takes time to improve extraction efficiency, and the extraction system must be heated to perform extraction without taking time. There is a drawback in that a condition that promotes decomposition is required for the fat-soluble component, such as.

  Regarding the production method (2), Patent Document 12 discloses a method for obtaining a high-concentration carotenoid-containing product from a processed vegetable and / or fruit product without using an organic solvent with a good yield. In this method, “a processed vegetable and / or fruit-containing product containing carotenoids is subjected to a solid-liquid separation process to remove a liquid part, and the liquid part is homogenized under high pressure to aggregate the carotenoid components contained therein, followed by centrifugation. The method for producing a high-concentration carotenoid-containing product characterized in that the carotenoid component is extracted by separation treatment ”and the precondition that“ the carotenoid is contained in the liquid part of the raw material ”is necessary. This production method is low in efficiency for a raw material in which carotenoids are hardly present, such as seaweed.

On the other hand, the method for separating polysaccharides from seaweed is a method in which seaweed is contacted with hot water, and then the laver is pulverized and extracted with water or alcohol (Patent Document 13), or a manufacturing method for extracting from water-soluble components (Patent Document) 14, Patent Literature 17, Patent Literature 18), a method of removing seaweed from an aqueous solution after contact with an aqueous alkali metal salt solution (Patent Literature 15), adding water from a seaweed strip or powder, and then wet polishing. A method of pulverizing and extracting (Patent Document 16) and a method of pulverizing Amanori seaweed and extracting it with hot water are disclosed (Patent Document 19). These are basically focused on polysaccharides of water-soluble components separated from seaweed.
"Carotenoids in Health and Disease", NI Krinsky, ST Mayne, H. Sies eds., Marcel Dekker: New York, 2004 H. Maeda et al., Biochemical and Biophysical Research Communications, 332 (2005) 392-397. Hosokawa, Bio Industry, 21 (2004) 52-57. T. Tsukui, et al., Journal of Agricultural and Food Chemistry, 55 (2007) 5025. I. Ikeda et al., Journal of Lipid Research, 29 (1988) 1573-1582. "AA, EPA, DHA-Highly unsaturated fatty acid" edited by Hikaru Kayama, Hoshiseisha Koseikaku Japanese Patent Laid-Open No. 10-158156 Japanese Patent Laid-Open No. 9-173012 Japanese Patent Laid-Open No. 9-173011 JP-A-8-3468 JP-A-8-70826 JP-T-2006-516293 JP 2006-701114 A JP 2004-89158 A Japanese Patent Laid-Open No. 2005-23028 JP 2004-2219 A Japanese Patent Laid-Open No. 2005-27520 JP 2006-57041 A JP 2002-65223 A JP 2002-212201 A JP 2002-105102 A JP 2004-49072 A JP 2005-102039 A JP 2005-225924 A Japanese Patent Laid-Open No. 11-113529

As described above, regarding the production method (1), the seaweed-derived fat-soluble oil obtained in each patent document has a high concentration of the fat-soluble oil itself, but uses an organic solvent. It is difficult to ensure safety that can be used as food, and there is a problem that the actual application is limited.
On the other hand, with respect to the production method (2), there is a problem that the method of Patent Document 12 is low in efficiency, for example, against seaweed.

  On the other hand, the methods described in each patent document relating to a method for separating polysaccharides from seaweed focus on the water-soluble polysaccharides separated from seaweed, and enable extraction of fat-soluble components contained in seaweeds. It was n’t.

  An object of this invention is to provide the novel seaweed origin product material which concentrated the bioactive component, without using an organic solvent, its manufacturing method, and a seaweed origin product.

As a result of earnest studies on the above problems, the present inventors have incubated the refined seaweed in distilled water, tap water, ground water, an aqueous solution adjusted in pH, or an aqueous alkali metal salt solution, and then liquid components. It was found that a seaweed-derived product material in which a fat-soluble oil containing various components exhibiting physiological activity was concentrated on a solid component obtained by separating into a solid component was obtained.
That is, by removing water-soluble components, particularly polysaccharides, which are the main components of seaweed, from refined seaweeds, a seaweed-derived product material in which fat-soluble oil is concentrated can be obtained, and without using organic solvents, The ratio of the fat-soluble oil can be increased, and it can be used for various applications including food.

  The method for producing a seaweed-derived product material of the present invention that solves the above problems includes (1) micronizing seaweed, (2) incubating the micronized seaweed in an aqueous enzyme solution, and (3) after incubation, The solid component is separated from the solid component, and (4) the solid component is dried.

  The seaweed-derived product material in which the physiologically active substance of the present invention is concentrated can be produced by the method for producing a seaweed-derived product material of the present invention.

  The seaweed-derived product material of the present invention can be added to obtain a seaweed-derived product in which physiologically active substances such as foods, beverages, supplements, and pet foods are concentrated.

  According to the present invention, a seaweed-derived product material enriched with a novel physiologically active substance can be provided. Moreover, the manufacturing method of the novel seaweed origin product raw material can be provided.

  Hereinafter, the embodiment of the present invention will be described in detail, but the present invention is not limited to this embodiment.

The said process (1) in the manufacturing method of the seaweed origin product raw material of this invention can apply the seaweed used as a raw material, and the water content (weight ratio) is 20% or less. As a drying method, a method of drying by natural force such as sun drying or a method of drying using an apparatus such as freeze drying, hot air drying, vacuum drying, drying by microwave irradiation may be used.
In general, when a solid is refined (powdered), the lower the water content of the solid, the more uniform the particle size and the smaller the average particle size. In the case of seaweed naturally dried in the shade (Wakame, Chigaiso, Aname, Macombu, Mitsushi Kombu, Umitoranoo, Akamok, Fusushimoku, Red Mozuku, Gagome Kombu, Hijiki, Fukurofunori), the seaweed weight change is balanced. The water content at that time was about 88% to about 91%. Even when using these naturally dried seaweeds, which is a gentle and low-cost drying method, a sufficiently fine seaweed powder could be obtained, so the water content (weight ratio) of the seaweed used as a raw material Is preferably 20% or less.

  When seaweed is dried at a low temperature, decomposition of the physiologically active substance in seaweed can be suppressed by performing vacuum freeze-drying. When seaweed is dried at a high temperature, it is necessary to dry it in a short time in order to avoid decomposition and carbonization of physiologically active substances in seaweed as much as possible. Furthermore, it is possible to refine the seaweed in a short time by incubating the seaweed in a supercritical fluid (in the case of water, the temperature is 374 ° C. or higher and 22.1 MPa or higher).

  The refinement of the seaweed in the step (1) can be performed by one or a combination of two or more selected from a pulverizer, an attritor, a homogenizer, a supercritical fluid machine, a subcritical fluid machine, and an ultrasonic generator. However, any means may be used as long as the seaweed is refined.

  In the step (1), the average particle size of the refined seaweed is desirably 1 μm to 5 mm. When the average particle size of the refined seaweed is less than 1 μm, it becomes very difficult to separate the solid component and the liquid component in the solution after the enzyme reaction. Moreover, when the average particle diameter of the refined seaweed exceeds 5 mm, it is disadvantageous in improving the efficiency of the enzyme reaction (reaction time, stirring, etc.).

As the aqueous solution in the step (2), water, a pH buffer solution, or an alkali metal salt aqueous solution can be used.
In the step (2), it is desirable that the pH of the aqueous solution is 5 to 9 in consideration of the stability of the physiologically active ingredient.
When the pH of the aqueous solution is less than 5, 5,6-epoxide in fucoxanthin becomes 5,8-epoxide, which is not preferable. On the other hand, when the pH of the aqueous solution exceeds 9, a decomposition product of fucoxanthin is generated. For example, according to Non-Patent Document 6, when fucoxanthin is added to a methanol solution of 0.01% potassium hydroxide (molar ratio of potassium hydroxide to fucoxanthin is 2.2), each carotenoid component ratio after 40 minutes is Fucoxanthin (33%), Fucoxanthinol (8%), Fucoxanthin hemiketal (11%), Fucoxanthinol hemiketal (3%), Isofucoxanthin (17%), Isofucoxanthinol (14 %).
J. et al. A. Haugan et al. , Acta Chemica Scandinavica 46 (1992) 614-624.

In the step (2), it is desirable that the incubation is performed at a temperature of 0 ° C. to 100 ° C. in consideration of the stability of the physiologically active ingredient.
When the incubation temperature is less than 0 ° C., the reaction solution freezes, and the efficiency of the enzyme reaction decreases (optimum temperature, the stirring efficiency decreases due to the viscosity of the extracted polysaccharide). When the incubation temperature exceeds 100 ° C., the degradation of the physiologically active component is promoted, and most of the enzymes that are a kind of protein lose their activity due to denaturation by heat.

In the step (2), it is desirable that incubation is performed for 10 minutes to 48 hours in consideration of the yield and stability of the physiologically active ingredient.
When the incubation time is less than 10 minutes, the extraction efficiency of water-soluble substances contained in seaweed into water decreases. If the incubation time exceeds 48 hours, degradation of the fat-soluble substance and bacterial growth may occur.

In the step (2), it is desirable that the weight ratio of the seaweed powder to the aqueous solution is 1: 100 to 1: 5 in consideration of the yield.
When the weight ratio of the seaweed powder to the aqueous solution is less than 1/100, it takes time to separate the solid component and the liquid component in the next step. When the weight ratio of the seaweed powder to the aqueous solution exceeds 1/5, the aqueous solution to be added before the incubation is reduced, the stirring is not smoothly performed, and the extraction efficiency of the water-soluble substance contained in the seaweed to water is lowered.

  In the step (3), the method of separating the reaction substance after incubation into a liquid component and a solid component can be performed by one or a combination of two or more selected from filtration, centrifugation, or decantation. As long as the liquid component and the solid component are separated, any means may be used.

In the step (4), it is desirable that the solid component is dried at a temperature of −50 ° C. to 100 ° C. in consideration of the manufacturing cost and the stability of the bioactive component.
For example, even if the temperature of the solid component is less than −50 ° C. during freeze-drying, an effect commensurate with the manufacturing cost cannot be obtained. Moreover, when the drying temperature of a solid component exceeds 100 degreeC, decomposition | disassembly of a fat-soluble substance is accelerated | stimulated.

In the step (4), the solid component may be dried at a pressure of 10 ⁻⁶ Pa to 100 MPa.
When the drying pressure of the solid component is less than 10 ⁻⁶ , a high vacuum device such as a turbo molecular pump or an ion pump is necessary, and it is not necessary to use these high vacuum devices for the purpose of simply drying seaweed.
In the case of a supercritical fluid of water, it is necessary to keep the reaction system at a temperature of 374 ° C. or higher and 22.1 MPa or higher. However, when the solid component drying pressure exceeds 100 MPa, the cost and running cost of making the reaction system robust An effect commensurate with is not obtained.

  In the step (4), the physiologically active component is a carotenoid, for example, auroxanthin, astaxanthin, asteroidone, adonixanthin, adonirumin, amarouciaxanthin. ), Alloxanthin, Antheraxanthin, Idoxanthin, Echinenone, α-carotene, β-carotene, γ-carotene, ζ Carotene (ζ-catonene), canthaxanthin (Chrysanthemaxanthin), cryptoxanthin (Cryptoxanthin), zeaxanthin, taraxanthin, tunaxanthin, diatoxanthin, diatoxanthin (Diatoxanthin) Nuros Polene (Neurosporene), α-Doradexanthin, Neoxanthin, Neochrome, Halocynthiaxanthin, Violaxnathin, Fucoxanthinol, Fucoxanthinol (Fucoxanthin), FrithiXanthin (Fritschiellaxanthin), Mythiloxanthinone (Mythiloxanthinone), Mytiloxanthin, Mutatoxanthin, Mutatochrome, Lycopene, Lutein It is desirable that they are luteoxanthin, rhodoxanthin, and the like.

In the step (4), the physiologically active substance is a sterol, for example, cholestanol, cholesterol (Cholesterol), latosterol (Lathosterol), 7-dehydrocholesterol, desmosterol, 22- Dehydrocholesterol (22-Dehydrocholesterol), Campestanol (Campestanol), 7-Dehydrocampesterol (7-Dehydrocampesterol), Ergostanol (Dihydrobrassicasterol), Fungisterol (Fungisterol) ) 22-Dihydroergosterol (22-Dihydroergosterol), Pincasterol (Pincasterol), Stellasterol (Brassicasterol), 5-Dihydroergosterol (5-Dihydroergosterol), Lugosterol (Ergosterol), Kodisterol (24-Methylenecholestanol), 24-Methylenecholesterol (24-Methylenecholesterol), Episterol (Episterol), Stigmastanol, Sitosterol, Stigmast-7-enol, 7-Dehydrositosterol, Cryonasterol, Dihydrochondrillastenol, 7-dehydroclionasterol, Stigmasterol, Spinasterol, Corbisterol, Polyferasterol, Chondrillasterol, Fucostanol, Fucostanol, Fucosterol), 28- iso Fukosu ethanol (28-Isofucostanol), 28- isophthalate Coste roll ^{(28-Isofucosterol), Δ 7} - avenasterol (Δ ⁷ -Avenasterol), it is desired that a Kurerosuteroru (Clerosterol) or the like.

  In the step (4), it is desirable that the physiologically active component is a fatty acid, for example, myristylic acid, palmitic acid, palmitoleic acid, γ-linolenic acid, linolenic acid, arachidonic acid, stearidonic acid, eicosapentaenoic acid, docosahexaenoic acid and the like. It is.

  Any seaweed may be used as long as the seaweed belongs to the class of brown algae (scientific name: Phaeophyta). For example, Aquintospora (scientific name: Acinetospora crinita), Tawaragatashimidoro (scientific name: Hincksia mitchellae), Matsumo (Scientific name: Analipus Japonicus), Isoiwatake (scientific name: Ralfsia verrucosa), Yotsudekurogashira (scientific name: Sphacelaria divaricata); (Scientific name: Dictyopteris punctata), Siwayahaz (scientific name: Dictyopteris undulata), ajijigusa (scientific name: Dictyota dichotoma), kazunoamiji (scientific name: Dictyota divaricata), itamiji (scientific name: Dictyota linearis), fukurimamioph (scientific name: oka Dil : Distromium decumbens), Giant Ogi (scientific name: Labo) phora variegata), Sanadagusa (scientific name: Pachydictyon coriaceum), sea urchin (scientific name: Padina japonica), Okinawa (scientific name: Padina minor), Atsuba common gussa (scientific name: ssum ), Jigmigusa (scientific name: Stypopodium zonale), Shima Ogi (scientific name: Zonaria diesingiana), Nisemozuku (scientific name: Acrothrix pacifica), Nagamatsumo (scientific name: Chordaria flageliformis), Okinawa mozuku (scientific name: Clodosiphon okamuranus) , Ishimozuku (scientific name: Sphaerotrichia divaricata), Futomozuku (scientific name: Tinocladia crassa), Ishige (scientific name: Ishige okamurae), Iroro (scientific name: Ishige sinicola), Nevaimo (scientific name: Leathesia difformis), Shiwanogo e (Scientific name: Nemacystus desipien s), Common Nagabukuro (scientific name: Asperococcus bullosus), Siwahige (scientific name: Myelophysus simplex), Ezozoburo (scientific name: Coilodesme cystoseirae), Fennel (scientific name: Dictyosiphon foeniculaceu), Habamodoki (scientific name: Punctaria latif phaeodactyla), Fukurofunori (scientific name: Colpomenia sinuosa), Gagomenori (scientific name: hydroclathrus clathratus), Habanori (scientific name: Petalonia binghamiae), cynomolgus (scientific name: Scytosiphon lomentaria), Queberigusa (scientific name: Cutleria tleca) , Hiramchimo (scientific name: Cutleria multifida), Ichimegasa (scientific name: Carpomitra costata), Quail (scientific name: Sporochunus radiciformis), Urushigusa (scientific name: Desmarestia ligulata), Tobacco rush (scientific name: Desmarestia tabacoides), gusta aria (Scientific name Alaria crassifolia), sea turtle (scientific name: Alaria fistulosa), Ainu sea turtle (scientific name: Alaria praelonga), green sea turtle (scientific name: Undaria peterseniana), wakame (scientific name: Undaria pinnatifida), Hirome (scientific name: Undaria undarioides), turmo (scientific name) filum), squirrel (scientific name: Agarum clathratum), catacombs (scientific name: Arthrothamnus bifidus), sujime (scientific name: Costaria costata), kajime (scientific name: Ecklonia cava), kurome (scientific name: Ecklonia kurome), tsuruarame (scientific name: Ecklonia stoifera) , Alame (scientific name: Eisenia bicyclis), Trollo comb (scientific name: Kjellmaniella gyrata), Gagara kombu (scientific name: Laminaria diabolica), Onikombu (scientific name: Laminaria japonica), Macombe (scientific name: Laminaria japonica), Nagacomo long (iss) (Scientific name: Laminaria yezonsis), Hibamata (scientific name: Fucus distichus), Ezoi Shige (scientific name: Silvetia babingtonii), Nebutomok (scientific name: Cystoseria crassipes), Joromoku (scientific name: Myagropsis myagroides), Fusizimoku (scientific name: Sargassum confusum), Futae-moku (scientific name: Sargassum duplicatum), hijiki (scientific name: sumi gas (sum) Scientific name: Sargassum hemiphyllum), Akamoku (scientific name: Sargassum horneri), Sawtooth moku (scientific name: Sargassum macrocarpum), Togemoku (scientific name: Sargassum micracanthum), Miyabemoku (scientific name: Sargassum miyabei), Tamahaumuma scientific name Sargassum nigrifolium), Tamanashimoku (scientific name: Sargassum nipponicum), Yatsumata moku (scientific name: Sargassum patens), Mametawala (scientific name: Sargassum ringgoldianum), Yoberamok (scientific name: Sargassum ringgoldianum), Yoremok (scientific name: Sargassum numonicum) , Yoremokumodoki (scientific name: Sargassum yamamotoi), Endoumoku (scientific name: Sargassum yendoi), Rappamoku (scientific name: Turbinaria ornata) can be as is at least one member selected from.

  Any seaweed may be used as long as the seaweed belongs to the green algae class (scientific name: Chlorophyta). For example, the seaweeds (scientific name: Collinsiella cava), human egusa (scientific name: Monostroma nitidum), hiraaonori ( Scientific name: Enteromorpha compressa), Bow Aonori (scientific name: Enteromorpha intestinalis), Usba Aonori (scientific name: Enteromorpha prolifera), Nagaaosa (scientific name: Ulva arasakii), Button Aosa (con name: glo) Scientific name: Ulva japonica, Anaaaosa (scientific name: Ulva pertusa), Amimoyo (scientific name: Microdictyon japonicum), Tanomogusa (scientific name: Microdictyon okamurae), Hosobaronia (scientific name: Valoniopsis pachynema), Hosojusmo (scientific name: Chaetomorpha cr Chaetomorpha moniligera), Futjuzumo (scientific name: Chaetomorpha sp) iralis), Giant plover (scientific name: Cladophora japonica), Chasiogusa (scientific name: Cladophora wrightiana), Aomogusa (scientific name: Boodlea coacta), Magamata (scientific name: Boergesenia forbesii), Tampoyari (scientific name: Chamaedoris orientalisop ), Fox fox (scientific name: Cladophoropsis vaucheriaeformis), buckwheat (scientific name: Dictyospaeria cavrnosa), tamabaronia (scientific name: Valonia aegagropila), valerian (scientific name: Ventricaria ventricosa), herai wazta (scientific name: chy lycopodium f. amicorum), Fujinohazuta (scientific name: Caulerpa fergusonii), Himezidazta (scientific name: Caulerpa filicoides), Kubirezta (scientific name: Caulerpa lentillifera), Fusaiwazuta (scientific name: Caulerpa okamurae), sennariata , Takatsukizuta (scientific name: Caulerpa racemosa var. Peltata), Cyhaizuta (scientific name: Caulerpa serrulata var. Boryana f. Occidentalis), Yorezta (scientific name: Caulerpa serrulata var. Serrulata f. Lata) , Kizamizuta (scientific name: Caulerpa subserrata), Ichizuta (scientific name: Caulerpa taxifolia), Kokeiwazuta (scientific name: Caulerpa webbiana f. Tomentella), Marbahauchiwa (scientific name: Avrainvillea obscura), Itogenomayusaki (scientific name: pitorus gu) discoidea), broad cactus (scientific name: Halimeda macroloba), cactus (scientific name: Halimeda opuntia), Suzukemo (scientific name: Tydemania expeditionis), Nanban Haimil (scientific name: Codium arabicum), Nagamil (scientific name: Codium cylindricum), mill (scientific name: Codium fragile), mottle mill (scientific name: Codium i ntricatum), Hiramil (scientific name: Codium latum), Tamil (scientific name: Codiuim minus), Cromyl (scientific name: Codium yezoense), Ohanemomo (scientific name: Bryopsis maxima), Mizutama (scientific name: Bornetella sphaerica) , Hudenoho (scientific name: Neomeris annulata), Ryukyugasa (scientific name: Acetabularia dentata), Kasanori (scientific name: Acetabularia ryukyuensis), isosugina (scientific name: Halicoryne wrightii).

  Any seaweed may be used as long as the seaweed belongs to the class of green algae (scientific name: Rhodophyta). Porphyra yezoensis), Katabenifukuronori (scientific name: Halosaccion firmum), Benifukuronori (scientific name: Halosaccion yendoi), Japanese black moth (scientific name: Dermonema pulvinatum), Japanese white-skinned (scientific name: Actinotrichia fragilis), Japanese lagerata Scientific name: Galaxaura obtusata), Nagara Ragara (scientific name: Galaxaura rugosa), Fusanori (scientific name: Scinaia japonica), Hirafu Sanori (scientific name: Scinaia latifrons), Nisefusanori (scientific name: Scinaia okamurae), Garinari (scientific name: Tr : Helminthocladia australis), Yogorekonahada (scientific name: Liagora japonica) , Cyprinus (scientific name: Nemalion vermiculare), Nurhada (scientific name: Trichogloeopsis mucosissima), Kaninote (scientific name: Amphiroa misancensis), Himekaninote (scientific name: Amphiroa zonata) (Scientific name: Corallina pilulifera), hiraitaisimo (scientific name: Lithophyllum bamleri), hiraibo (scientific name: Lithophyllum okamurae), molkai shimo (scientific name: Lithophyllum pygmaeum) (scientific name: Lithhothamnion cystocarpideum) : Marginisporum aberrans), Ishinohana (scientific name: Mastophora rosea), Yuikiri (scientific name: Acanthopeltis japonica), Eurasian primrose (scientific name: Gelidiella acerosa), Moxa (scientific name: Gelidium elegans), Onyxa (scientific name: Gelidium japonicum) ladiella tenuis), Hirakusa (scientific name: Ptilophora subcostata), Kagikenori (scientific name: Asparagopsis taxiformis), Isodantsu (scientific name: Caulacanthus ustulatus), Namiitaketake (scientific name: Tylotus lichenoides), Oobaokitsubara (scientific name: life in the red) Dudresnaya japonica), Rattlefish (scientific name: Rhodopeltis borealis), Hanafnori (scientific name: Gloiopeltis complanata), Fukurofunori (scientific name: Gloiopeltis furcata), Mahonori (scientific name: Gloiopeltis tenax), Suscabenn (scientific name: tis intermedia), Suginori (scientific name: Chondracanthus tenella), Kotojitsu no Mata (scientific name: Chondrus nipponicus), Tsunomata (scientific name: Chondrus verocultus), Hiratokotoji (scientific name: Chondrus vern s), black-crowned nansou (scientific name: Chondrus yendoi), red-skinned nansou (scientific name: Mazzaella japonica), matsunori (scientific name: Carpopeltis affinis), rice bran (scientific name: Carpopeltis prolifera), Nikumukade (scientific name: Grateloupia carnosa), : Grateloupia elliptica), Centipede (scientific name: Grateloupia fillicina), Fudarak (scientific name: Grateloupia lanceolata), Kyono Himo (scientific name: Grateloupia okamurae), Filigusa (scientific name: Halymenia dilatata), Tsunomukade (scientific name: P crispata), Pinus pine (scientific name: Prionitis divaricata), Suimdekade (scientific name: Prionitis ramosissima), Ibaranori (scientific name: Hypnea charoides), Kazunobara (scientific name: Hypnea flexicaulis), Kagyi baranori (scientific name: Hypnea japonica), Saibara (scientific name: Hypnea japonica) , Tachiibara (Scientific name: Hypnea var iabilis), Nezashinotosakamodoki (scientific name: Callophyllis adnata), Hosobanotosakamodoki (scientific name: Callophyllis japonica), Kinu Hada (scientific name: Callophyllis okamurae), Ezotosaka (scientific name: Circirlicarpus gmelini), Iborito (Scientific name: Peyssonnelia caulifera), Kinokawa (scientific name: Peyssonnelia japonica), Saimi (scientific name: Ahnfeltiopsis flabelliformis), Hoso Yukari (scientific name: Plocamium cartilagineum) : Portieria hornemannii), Naminohana (scientific name: Portieria japonica), catamen giraffe (scientific name: Betaphycus gelatinum), giraffe (scientific name: Eucheuma denticulatum), Tosakanori (scientific name: Meristotheca papulosa), mirin (scientific name: Solieria pacifica) Gracilaria b ursa-pastoris), Giant tiger moth (scientific name: Gracilaria gigas), Mizoogonori (scientific name: Gracilaria salicornia), Birch (scientific name: Gracilaria textorii), Ogonori (scientific name: Gracilaria vermiculophya physic) bifida), Hera myrtle (scientific name: Champia japonica), Fusutsunagi (scientific name: Lomentaria catenata), Kosushi fusunagi (scientific name: Lomentaria hakodatensis), Kamensou (scientific name: Ceratodictyon spongiosum), Sugikonisori (scientific name: chemaebosio) , Tengu-Smodoki (scientific name: Gelidiopsis repens), Anadals (scientific name: Sparlingia pertusa), Egonori (scientific name: Campylaephora hypnaeoides), Amyssa (scientific name: Ceramium boydenii), Igis (scientific name: Ceramium kondoi), Keigis (scientific name: rim) Mosquito Wabenihiba (scientific name: Neoptilota asplenioides), Benihiba (scientific name: Psilothallia dentata), Langeria (scientific name: Wrangelia tanegana), Kagiusubanori (scientific name: Acrosorium venulosum), Ayanishiki (scientific name: Martensia fragilisor costoli) Scientific name: Acanthophora spicifera), Bentenmo (scientific name: Benzaitenia yenoshimensis), Kokemodoki (scientific name: Bostrychia tenella), Yuna (scientific name: Chondria crassicaulis), Beniya naginori (scientific name: Chondria ryukyuensis), cinnoha (scientific name: acc Digenea simplex), Kurosozo (scientific name: Laurencia intermedia), Mitsudezozo (scientific name: Laurencia okamurae), Magirezozo (scientific name: Laurencia saitoi), Kobusoso (scientific name: Laurencia undulata), Itofuji pine (scientific name: Neorhodomela munno) Scientific name: Odonthalia annae ), At least one kind selected from Scutellaria (scientific name: Odonthalia corymbifera).

  Examples of foods that are made from the seaweed-derived product material of the present invention to produce seaweed-derived products include noodles, spices, coloring agents, confectionery, rice cakes, jelly, and sprinkles.

  Beverages obtained by adding the seaweed-derived product material of the present invention to obtain seaweed-derived products include alcoholic beverages, soft drinks, spices, and coloring agents.

  Supplements to which the seaweed-derived product material of the present invention is added to obtain seaweed-derived products include anti-obesity agents, blood sugar level increase inhibitors, DHA / EPA synthesis promoters, cancer cell growth inhibitors and the like.

  Examples of animal foods that are obtained by adding the seaweed-derived product material of the present invention to produce seaweed-derived products include pet food for dogs and cats, feed for cattle, pigs, sheep, and birds, and feed for tropical fish and farmed fish.

Examples of the method for producing the seaweed-derived product material of the present invention will be described below.
Example 1
This embodiment will be described with reference to seaweed that is widely used in Japan as a raw material for seaweed-derived product materials.
The seaweed-derived product material in which the physiologically active substance is concentrated is manufactured according to the manufacturing flowchart shown in FIG.

The wakame raw material used in this example is three kinds of dried wakame powder (first is a powder obtained by refining dried cut wakame with a commercially available food mixer and passing through a sieve of 106 μm. The second is pulverized. A powder that is made from dried cut seaweed using an atomizer (Masuko Sangyo Co., Ltd.) and has an average particle size of 27.5 μm, and the third is a dry cut using a selenium mirror (Masuko Sangyo Co., Ltd.). Wakame refined powder with an average particle size of 30.1 μm 4 Paste wakame (raw wakame before drying dry cut wakame made into a paste using mascolloyer (Masuko Sangyo Co., Ltd.)) 4 It is a kind.
In the results of the examples shown in the following figures, (a) Wakame refined with an atomizer, (b) Wakame refined with a selenium mirror mill, (c) Finely refined with a commercial food mixer, 106 μm or less was sieved. The case where collected wakame and (d) paste wakame are used as raw materials is shown.

  A method for concentrating a physiologically active substance (particularly a fat-soluble substance) from 5 g of the refined seaweed obtained by the above method (in terms of dry weight) is shown below. 150 mL of distilled water was added to 5 g of each of the four types of seaweed raw materials (the paste wakame was 150 mL of distilled water including the water contained in the raw materials) and incubated at 37 ° C. for 1 hour. Here, seaweed cell wall degrading enzyme (Alginate lyase from Flavobacterium sp, manufactured by Sigma) was added, and the others were also examined for those incubated under the same conditions as described above.

The reaction product obtained after the incubation was centrifuged (5000 g, 10 minutes) and separated into a solid component (bioactive substance-enriched seaweed material) and a liquid component (main component: polysaccharide).

  The water-soluble component is concentrated by adding an equal amount (v / v) of ethanol to precipitate the polysaccharide, thereby concentrating the bioactive component (fat-soluble substance) contained in the solid component (bioactive substance-enriched seaweed material). It was an index for rate. That is, the concentration ratio of the physiologically active substance was examined by comparing the weights of the removed polysaccharide fractions. In addition, in the case of the paste-like seaweed which does not use the seaweed cell wall decomposing enzyme, it performed by 40000g and 10min.

  Fig. 2 is a photograph of a lyophilized solid component obtained by incubating without using seaweed cell wall degrading enzyme and then centrifuging, and Fig. 3 incubating without using seaweed cell wall degrading enzyme and then centrifuging. It is a photograph of the freeze-dried product obtained by adding ethanol to the obtained liquid component and freeze-drying the deposited precipitate.

  FIG. 4 is a photograph of a lyophilized solid component obtained by incubation using a seaweed cell wall degrading enzyme followed by centrifugation, and FIG. 5 is a photograph of incubation using a seaweed cell wall degrading enzyme followed by centrifugation. It is a photograph of the freeze-dried liquid component obtained by adding ethanol to the obtained liquid component and freeze-drying the deposited precipitate.

  Comparison of solid components that do not use enzymes (Fig. 2) and those that use enzymes (Fig. 4) show that white spots are conspicuous in those that do not use enzymes, whereas those that use enzymes There were many green spots. This green color is derived from chlorophyll, which is one of the fat-soluble components, and it was expected that the fat-soluble component was more concentrated when the enzyme was used. In addition, when the liquid component not using the enzyme (FIG. 3) and the liquid component using the enzyme (FIG. 5) were compared, the one that did not use the enzyme appeared slightly more green. It was found that the liquid component (polysaccharide fraction) was often made from paste-like seaweed as a raw material both in the case where the enzyme was not used and the case where the enzyme was used.

The results of actually measuring the weight of the solid component and the liquid component (polysaccharide fraction) are shown in FIGS.
FIG. 6 is incubated without using seaweed cell wall degrading enzyme, and FIG. 7 is incubated using seaweed cell wall degrading enzyme. In both cases, the solid components obtained by centrifuging are freeze-dried, and the physiologically active components are concentrated. The weight of the wakame material (outlined) and polysaccharide fraction (hatched) are shown.

  Compared to the four kinds of wakame raw materials, solid ingredients (wakame physiologically active substance concentrated material), the ones that use pasty wakame as the raw material are the most, regardless of whether the enzyme is used or not. A polysaccharide fraction was obtained. That is, the polysaccharide fraction not containing the wakame physiologically active substance (fat-soluble component) could be efficiently removed. In particular, it was found that when a paste-like wakame was used as a raw material under conditions where no enzyme was used, a polysaccharide fraction comparable to that obtained when a dry wakame powder using an enzyme was used as a raw material was obtained. Therefore, when the raw material is refined, the polysaccharide fraction can be removed from seaweed with high efficiency by making the raw material water-containing.

(Example 2) Fat-soluble component, fucoxanthin and fucosterol content in wakame enriched with physiologically active substance Fat-soluble component, fucoxanthin and fucosterol in wakame enriched with physiologically active substance according to the production flowchart shown in FIG. The content was examined. The details will be described below.
Four types of bioactive substance-enriched wakame materials (obtained by incubation with and without using 5 g of 4 types of refined wakame as raw materials) and the raw material itself (5 g) as a comparison The same operation was performed for.

First, 200 mL of ethanol was added to the wakame material and raw materials, and the fat-soluble components were extracted for 24 hours at room temperature under light shielding.
This was separated into a solution component and a solid component by filter filtration. Next, 200 mL of ethanol was again added to the solid component, and the wakame fat-soluble component was extracted for 24 hours at room temperature under light shielding, and this was separated into a solution component and a solid component by filter filtration. Finally, the solution components obtained in each were combined into one, concentrated under reduced pressure with an evaporator, and nitrogen gas was blown onto the resulting concentrate to completely remove the organic solvent. The weight of the obtained concentrate was measured with an electronic balance, and this was taken as the weight of the wakame fat-soluble component. This concentrate was dissolved in a developing solvent (see the following HPLC measurement conditions) used in high performance liquid chromatography (HPLC). The HPLC measurement conditions are Develosil ODS-UG5 (column), 30% acetonitrile / methanol (developing solvent), 1 mL / min (flow rate), 28 ° C. (column temperature), 450 nm (detection wavelength). Hereinafter, the results will be described.

  8 shows the ratio (%) of the fat-soluble component per unit material weight, FIG. 9 shows the ratio (%) of fucoxanthin contained per unit material weight, and FIG. 10 shows the fucosterol contained per unit material weight. The percentage (%) is shown. In each figure, (a) Wakame refined with an atomizer, (b) Wakame refined with a selenium mirror, (c) Wakame refined with a commercial food mixer and collected with a sieve of 106 μm or less, (d) Paste The case where wakame is used as a raw material is shown. The white columns are the raw materials themselves, the shaded columns are those obtained by lyophilizing the solid components obtained by centrifuging after incubation without using enzymes, and the black columns are incubated using enzymes. The case where what was obtained from the freeze-dried solid component obtained by subsequent centrifugation is shown.

  Regarding the ratio (%) of the fat-soluble component contained per unit material weight shown in FIG. 8, when comparing the weight of the fat-soluble component between the raw material itself, the enzyme-free and the enzyme use, as shown in Table 1. In either case, the use of the enzyme> the raw material itself> no enzyme was used in the order of any refined seaweed.

  Moreover, even when converted to the ratio of the fat-soluble component per unit raw material weight, the enzyme was used> the raw material itself≈the enzyme was not used (in the paste raw material, the enzyme was not used> the raw material itself). In addition, it was found that about 2.0 times to 3.1 times more fat-soluble component can be extracted when using the enzyme than the raw material itself (about 8.9 times for the paste raw material).

Regarding the ratio of fucoxanthin contained per unit material weight shown in FIG. 9, when comparing the fucoxanthin weight between the raw material itself, no enzyme used, and the enzyme used, as shown in Table 1, excluding the paste raw material In this order, the raw materials themselves> enzyme use> no enzyme use. However, when converted to the ratio of fucoxanthin contained per unit material weight, enzyme use> raw material itself≈no enzyme used (in the paste raw material, enzyme use> no enzyme use> raw material itself).
In addition, it was found that fucoxanthin can be extracted about 1.6 to 1.8 times more in the case of using the enzyme than the raw material itself (about 5.3 times for the paste raw material).

  Furthermore, regarding the ratio (%) of fucosterol contained per unit material weight shown in FIG. 10, when the weight of fucosterol is compared between the raw material itself, the enzyme non-use, and the enzyme use, as shown in Table 1. In the case of wakame refined with an atomizer or a selenium mirror, the raw material itself was approximately in the order of enzyme use> no enzyme use. In the case of the food mixer, the raw material itself> the enzyme used> no enzyme used, and in the case of the paste raw material, the enzyme used> the enzyme used> the raw material itself. However, when converted to the ratio of fucosterol contained per unit material weight, the enzyme was used> the enzyme was not used ≒ the raw material itself (in the paste raw material, the enzyme was used> the enzyme was not used> the raw material itself). In addition, it was found that fucosterol can be extracted about 1.9 times more when the enzyme is used compared to the raw material itself (about 5.3 times as much as the paste raw material).

  Summarizing the above results, when the wakame is refined and incubated with an enzyme, the fat-soluble component, fucoxanthin, is converted into per unit material weight compared to when incubated without using the raw material itself or the enzyme. It was found that the amount of fucosterol extracted increased. Therefore, it was shown that a seaweed-derived product material in which functional components are effectively concentrated can be obtained by using this method.

  The present invention relates to a seaweed powder enriched with a physiologically active substance, a method for producing the same, and a seaweed-derived product. Therefore, the industrial applicability can be used as a functional food material by adding seaweed powder enriched with physiologically active substances to food materials, etc. as well as in the medical field. We can expect utilization.

The manufacturing flowchart of the seaweed origin product raw material which concentrated the bioactive substance of the Example of this invention. A photograph of a freeze-dried solid component obtained by incubating without using a seaweed cell wall degrading enzyme and then centrifuging. A photograph of a freeze-dried liquid component obtained by incubating without using a seaweed cell wall degrading enzyme and then centrifuging. A photograph of a freeze-dried solid component obtained by incubation using a seaweed cell wall degrading enzyme followed by centrifugation. A photograph of a freeze-dried liquid component obtained by incubation using a seaweed cell wall degrading enzyme followed by centrifugation. The figure which shows the result of having measured the weight of the solid component (seaweed raw material) and the liquid component (polysaccharide fraction) in Example 1 of this invention. The other figure which shows the result of having measured the weight of the solid component (seaweed material) and the liquid component (polysaccharide fraction) in Example 1 of this invention. Explanatory drawing which shows the ratio (%) of the fat-soluble component contained per unit raw material weight measured in Example 2 of this invention. Explanatory drawing which shows the ratio (%) of the fucoxanthin contained per unit material weight. Explanatory drawing which shows the ratio (%) of the fucosterol contained per unit raw material weight.

Claims (13)

A method for producing a seaweed-derived product material containing a physiologically active substance comprising any one fat-soluble component belonging to carotenoids, sterols and fatty acids, comprising the following steps (1) to (4) A method for producing derived product materials.
(1) Refine seaweeds belonging to the class of brown algae (scientific name: Phaeophyta).
(2) Incubating the refined seaweed in an enzyme aqueous solution containing a seaweed cell wall degrading enzyme at a temperature of 0 ° C. to 100 ° C. for 10 minutes to 48 hours.
(3) After incubation, the liquid component is separated from the solid component.
(4) The solid component is dried to obtain a seaweed-derived product material.   The method for producing a seaweed-derived product material according to claim 1, wherein the step (1) is a step of refining dried seaweed.   The method for producing a seaweed-derived product material according to claim 2, wherein the seaweed has a moisture content (weight ratio) of 20% or less.   The method for producing a seaweed-derived product material according to claim 1, wherein the step (1) is a step of refining seaweed containing 80% or more of water (weight ratio).   The method for producing a seaweed-derived product material according to any one of claims 1 to 4, wherein the step (1) is a step in which the seaweed is refined so that the average particle size of the seaweed is 1 μm to 5 mm.   The method for producing a seaweed-derived product material according to any one of claims 1 to 5, wherein the enzyme aqueous solution is at least one of distilled water, tap water, ground water, a pH buffer solution, and an alkali metal salt aqueous solution.   The method for producing a seaweed-derived product material according to any one of claims 1 to 6, wherein the pH of the aqueous enzyme solution is 5 to 9.   The method for producing a seaweed-derived product material according to any one of claims 1 to 7, wherein in the step (2), the weight ratio of the seaweed powder to the aqueous enzyme solution is from 1: 100 to 1: 5.   9. The process according to any one of claims 1 to 8, wherein in the step (3), the reactant after incubation is separated into a liquid component and a solid component by one or a combination of two or more selected from filtration, centrifugation, and decantation. The manufacturing method of the seaweed origin product material of description.   The method for producing a seaweed-derived product material according to any one of claims 1 to 9, wherein in the step (4), the solid component is dried at a temperature of -50 ° C to 100 ° C. The method for producing a seaweed-derived product material according to any one of claims 1 to 10, wherein in the step (4), the solid component is dried at a pressure of 10 ^-6 Pa to 100 MPa.   A seaweed-derived product material enriched with a physiologically active substance, which is obtained by the method for producing a seaweed-derived product material according to any one of claims 1 to 11. A seaweed-derived product obtained by adding the seaweed-derived product material according to claim 12.

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