JP5390469B2 - Low antigenic and highly digestible feed material and method for producing the same - Google Patents

Description

  The present invention is a feed raw material that is excellent in digestibility and palatability for infant animals and has high safety and can be used as a substitute for skim milk powder, a production method thereof, a feed containing the feed raw material, and a sugar for producing the feed raw material Quality solution.

  Infants are fed a feed mainly composed of skim milk powder. Fish powder, wheat protein, soybean protein, and the like are sometimes used as substitutes for skim milk powder due to the recent increase in feed prices due to a shortage of raw materials for skim milk powder. However, these substitutes have a problem that they are less preferred than skim milk powder and have a low nutritional value.

  It is known that when defatted soybeans are treated with a biaxial extruder, the antigenicity is reduced and the utility is increased (Patent Document 1: Raw material for infant animal feed). Still, the digestibility in pigs (Japanese standard feed ingredient table 2001 version) is 91% crude protein, 0% crude fat, 99% NFE, and 82% crude fiber, and further improvement of digestibility and palatability is desired. It is.

  Sweetened heat-treated soybean oil residue and humidified heat-treated soybean oil residue are also registered as feed raw materials. In either case, the digestibility is not so high, and it is inferior to the processed extruder.

  As a method for enhancing the palatability of the feed, flavors, sugars and the like are added (Patent Document 2: Preference feed material). Molasses and sugar-containing liquids generated during the production of sugar have a high preference for animals. However, since these are liquids, it is necessary to work directly on the bait when feeding. This limits use on the farm and makes it difficult to use. Moreover, when water | moisture content is high when producing a compound feed, since it will become a cause of mold | fungi and rot, the compounding quantity is restrict | limited to several% or less. Making the liquid into a powder by an operation such as spray drying or granulation drying causes a loss of flavor, is expensive, and is not suitable for feed.

Japanese Patent No. 2654889 Japanese Patent Laid-Open No. 5-76292

  Therefore, an object of the present invention is to provide a safer non-fat dry milk substitute that reduces anti-nutritional components such as antigenicity of defatted soybean, is excellent in digestibility and palatability, and has high safety.

  In order to solve the above-mentioned problems, the present inventors are examining the processing conditions of defatted soybeans, and neutral detergent insoluble protein (NDIPP), which is an analysis method for cattle feed, is an index of digestibility. I found. NDIP is used as an index for analyzing the amount of protein used by rumen microorganisms, and the higher the NDIP, the nutrition of the cow itself rather than the rumen microorganisms. Conventionally, feed has been studied for the purpose of producing a product having a high NDIPP. On the other hand, the present inventors speculated that the lower the value of NDIP, the higher the digestibility, and examined the development of a feed with a low NDIP.

  When sugar is added to defatted soybeans and heated and pressurized and kneaded, the rumen bypass property (reach rate to the small intestine) of the defatted soybeans increases, and with this, it should be expected that NDIPC also increases. However, the present inventors have surprisingly found that when a certain amount of sugar is added to defatted soybeans and heat-pressed and kneaded, the feed is surprisingly low in antigenicity and low in NDICP. Based on this finding, we succeeded in producing an alternative raw material for skimmed milk powder that has the same or better palatability as skim milk powder and is excellent in digestibility. That is, the present invention has a antigenicity of 100 U / 10 mg or less, obtained by adding a sugar solution to defatted soybean so that the sugar content is 7 to 24% by weight and subjecting to heat and pressure kneading. Provided is a feed material characterized in that its high digestibility is 30% or less as a neutral detergent-insoluble protein.

  In the present specification, the saccharide content means the ratio (% by weight) of added saccharide (dry matter weight) to the total of defatted soybean and added saccharide (dry matter weight). Similarly, an addition rate means the ratio (weight%) of the added carbohydrate solution with respect to the sum total of defatted soybean and the added carbohydrate solution.

  The present invention also provides a low-antigenic and highly-digestible feed material obtained by adding a saccharide solution to defatted soybean so as to have a saccharide content of 7 to 24% by weight, followed by heat and pressure kneading. Provide a method.

  The present invention also provides a feed comprising the low antigenic and highly digestible feed raw material so that the carbohydrate content derived from the feed raw material is 0.07 to 12% by weight of the total feed.

  The present invention is also a saccharide solution added to the defatted soybean so as to have a saccharide content of 7 to 24% by weight, and the antigenicity is 100 U / 10 mg or less by heat and pressure kneading after the addition, And the said carbohydrate solution for manufacturing the feed raw material whose highly digestible is 30% or less as neutral detergent insoluble protein is provided.

  The low antigenic and highly digestible feed material of the present invention is excellent in digestibility and nutritional value, and has high palatability. In particular, even if it is partially or completely replaced with skim milk powder, it has a nutritional value and palatability equivalent to or higher than those for young animals.

The structural example of the biaxial extruder for manufacturing the low antigenicity and highly digestible feed raw material of this invention is shown. In the figure, L is an L-type kneading disk, R is an R-type kneading disk, F is a forward screw, P is a pineapple screw, and a numerical value indicates a length (mm) for each block. The change of NDIPC (%) with respect to the PF-S addition rate when a low antigenic and highly digestible feed material is produced by adding PF-S using the biaxial extruder of FIG. 1 is shown. The change of pepsin digestibility (%) with respect to the PF-S addition rate when a low-antigenic and highly digestible feed material is produced by adding PF-S using the biaxial extruder of FIG. . It is the graph which measured the feed intake of the mouse | mouth over Example 5 in Example 14 (basic feed + this invention product) and the comparative example 1 (basic feed + biaxial EX process defatted soybean). As for feed intake, there is little difference between Day 1 and Day 2, and a significant difference appears after Day 2. In Example 15 (basic feed + biaxial EX-treated defatted soybean + product of the present invention) and Comparative Example 2 (basic feed + biaxial EX-treated defatted soybean), it is a graph obtained by measuring the feed intake of mice over 5 days. As for feed intake, there is little difference between Day 1 and Day 2, and a significant difference appears after Day 2. It is the graph which measured the feed intake of the mouse | mouth over Example 5 in Example 16 (basic feed + this invention product) and the comparative example 3 (basic feed + biaxial EX process degreasing soybean + skim milk powder + sugar).

  Hereinafter, the present invention will be described in more detail with reference to embodiments of the present invention. The low antigenic and highly digestible feed material of the present invention is obtained by adding a saccharide solution to defatted soybean and subjecting it to heat and pressure kneading.

  The defatted soybean is a soybean protein obtained by removing oil from soybean to usually 4% by weight or less. Since defatted soybean has a relatively excellent amino acid balance and is inexpensive, it is suitable for feed applications. The method for degreasing soybeans is not particularly limited, for example, by mechanical extraction or solvent extraction. The defatted soybean contains about 40-55% protein depending on the defatting method, variety, harvest region, year and the like. In the present specification, a protein content of 45 to 55% may be referred to as HP defatted soybean, and a protein content of 40 to 48% may be referred to as LP defatted soybean. In the present invention, commercially available defatted soybeans (for example, product name, defatted soybean low-pro, and defatted soybean high-pro, both manufactured by J-Oil Mills) can also be used.

  The sugar includes fermented milk (including fermented milk added with sugar), fermented milk-containing material, lactic acid fermented skim milk, lactose, sugar, sucrose, fructose, glucose, glucose hydrate, erythritol, mannitol , Palatinose, reduced palatinose, powdered reduced maltose, molasses, water candy, corn syrup, corn steep liquor, carmellose, sorbitol, xylitol and the like.

  Particularly preferred carbohydrate is fermented milk or fermented milk-containing material. Fermented milk can be produced, for example, by the method described in Japanese Patent Publication No. 6-55106. The contents described in Japanese Patent Publication No. 6-55106 are cited in the present specification for reference, and the outline is described below. Fermented milk is obtained by inoculating defatted animal milk collected from cattle, sheep, goats, and the like with lactic acid bacteria, or lactic acid bacteria and yeast, and lactic acid fermentation. Lactic acid bacteria to be used are not particularly limited as long as they can grow in the presence of animal milk. ) Genus and Bifidobacterium genus. Examples of yeast include Saccharomyces cerevisiae, Candida utilis, and Klyveromyces lactis.

  When the fermented milk is produced by two-stage fermentation, 100 parts by weight of animal milk is inoculated with 1 to 5 parts by weight of lactic acid bacteria as a starter and subjected to primary fermentation, for example, about 16 to 48 at 25 to 45 ° C. Ferment for hours. Suitably, saccharides such as sucrose, glucose and invert sugar are added in an amount of 15 to 50% by weight based on the primary fermented milk. Next, secondary fermentation is performed at 15 to 30 ° C. for 15 to 25 hours, for example. In the present invention, commercially available fermented milk (for example, product name PF-S, manufactured by Calpis) can also be used.

  The saccharide content of the saccharide solution is usually 30 to 70% by weight, preferably 40 to 60% by weight, more preferably 45 to 55% by weight. The carbohydrate solution is preferably fermented milk or fermented milk-containing material. Sugars such as fermented milk can be adjusted by adding sugar as necessary.

  The amount of the saccharide solution added to the defatted soybean is such that the saccharide content is 7 to 24% by weight, preferably 10 to 20% by weight, more preferably 12 to 17% by weight.

  As feed raw materials to be subjected to heat and pressure kneading treatment, in addition to the carbohydrates and defatted soybeans, those known in the art according to the type of livestock can be used as long as the effects of the present invention are not impaired. is there.

The temperature (product temperature) during the heat and pressure kneading treatment may be usually 120 to 400 ° C, preferably 220 to 270 ° C. If the temperature is lower than 120 ° C., the reduction of the anti-nutrient component is insufficient, and conversely if it is higher than 400 ° C., the protein or amino acid as the nutrient component may be destroyed. Moreover, 2-50 kg / cm < ² > may be sufficient as a pressure normally, Preferably it is 5-35 kg / cm < ² >. In addition, if the pressure is lower than 2 kg / cm ^2, the reduction of anti-nutritive components is insufficient, and conversely if it is higher than 50 kg / cm ² , the discharge from the apparatus becomes difficult and may cause scorching or clogging. . The time required for the heat and pressure kneading treatment is usually 10 seconds to 30 minutes, preferably 15 seconds to 2 minutes, although it depends on temperature and pressure.

  For the heat and pressure kneading treatment of the feed material, a feed production apparatus such as an expander, a single screw extruder or a twin screw extruder can be used. In this specification, the extruder may be referred to as EX. In the biaxial extruder, the maximum temperature reaching point of the cylinder is usually 120 ° C to 400 ° C, preferably 250 ° C to 370 ° C. In order to achieve this, it is advantageous to reduce the amount of carbohydrate solution at the time of start-up and increase the amount added when the temperature rises. In addition, it is preferable that a pineapple screw is inserted in the middle of the shaft and a kneading disk is inserted at the tip, from the viewpoint of uniform heating and pressure kneading treatment.

  The defatted soybean and sugar solution put in the extruder are heated, pressurized and kneaded with appropriate humidification while being stirred with a screw, and extruded from the hole of the die. The feed material extruded from the die is granulated with a cutter and dried as appropriate. You may grind | pulverize after drying.

  The low antigenicity and highly digestible feed material of the present invention and the blended feed blended with the feedstock have a low NDICP, which is an index of digestibility, while suppressing the antigen amount to 100 U / 10 mg or less. In the conventional sweetened heat-defatted soybean (Soypath), the antigen amount is as high as 150 U / 10 mg. Even if sweetening and heating conditions are increased to reduce the amount of antigen, the amount of antigen will not be less than 100U / 10mg unless burned and unfit for feed, and NDIP will be as high as 36%, for example. Is considered to be a tendency to deteriorate digestibility for monogastric animals and young animals. On the other hand, although the numerical value of NDIP of the feed raw material of the present invention may vary depending on the heat and pressure kneading processing machine (for example, extruder machine) employed, it is 30% or less, particularly 23% or less, which is lower than the conventional one. .

As an example of the NDIP measurement method, a method using a fiber analyzer (product name Fiber Analyzer A200, manufactured by ANKOM) is shown below. The procedure is as follows.
(1) Measure the weight of the filter bag.
(2) A sample is accurately measured in a filter bag by about 0.5 g.
(3) Heat seal. Ensure that the sample spreads evenly in the bag.
(4) Set the filter bag in the filter bag holder and place it in the fiber analyzer pot.
(5) Add 2 L of neutral detergent solution and 4 ml of thermostable α-amylase into the kettle, cover the kettle, press the Agitate and HEAT switches, and heat with stirring for 75 minutes.
(6) After heating, drain the solution, open the lid, add 2 L of boiling water and 4 ml of α-amylase, and press the AGIATE switch. Stir for about 10 minutes. After 10 minutes, the liquid was discharged and the same operation was repeated twice (total 3 times).
(7) Add 2 L of boiling water, press the AGIATE switch, and stir for about 10 minutes to discharge the liquid. This operation is repeated twice more (total 3 times).
(8) Take out the filter bag holder and remove the water remaining in the filter bag by pushing the filter bag.
(9) Put the filter bag in a beaker, pour acetone and let stand for 5 minutes. After 5 minutes, discard the acetone. Repeat the same operation (twice in total).
(10) The acetone remaining in the filter bag is removed by pushing the filter bag.
(11) Allow the filter bag to air dry for 1 hour.
(12) The filter bag is dried under reduced pressure at 105 ° C. for 4 hours.
(13) After drying, let stand overnight (return from dry weight to actual weight) and weigh.
(14) The protein content of the substance in the filter bag is measured using a Sumigraph (manufactured by Sumika Chemical Analysis Co., Ltd.).
(15) NDICP is calculated from the filter bag weight, sample weight, post-treatment weight, and post-treatment protein content.

Next, a method for measuring pepsin digestibility will be described.
(1) A sample is accurately measured, for example, 0.5 g, and placed in a 100 ml Erlenmeyer flask with a stopper.
(2) Add 75 ml of pepsin solution (pepsin (1: 10000) 2 g / 0.075 M HCl 1 L) heated to 45 ° C.
(3) After covering, react for 16 hours at 45 ° C. with shaking.
(4) After the reaction, the mixture is filtered with a filter paper (5A, 90 mm), and the residue is washed with warm water.
(5) After washing, dry at 60 ° C. for 48 hours.
(6) After drying, measure the protein content of the residue together with the filter paper using a smograph.
(7) The pepsin digestibility per protein is calculated from the protein content of the filter paper weighed in advance, the protein content of the sample, the sample weight and the protein content after degradation.

  Soybeans contain antigenic proteins (glycinin, β-conglycinin, etc.) that cause allergies. The antigenicity of defatted soybean is usually 10,000 U / 10 mg. When normal heat treatment is applied to defatted soybeans, antigenicity decreases, but NDCP increases. At the time of this treatment, even if the antigenicity is adjusted to 100 U / 10 mg or less by adjusting the amount of water and the temperature, the antigenicity is not sufficiently lowered, and NDIP exceeds 30%. Furthermore, when carbohydrate is added in the same treatment, the antigenicity tends to be lowered, but NDIP is further increased, and excessive heating causes burning. For example, in the autoclave treatment, the pressure is low and kneading is not performed, so that the antigen is not sufficiently reduced and NDIP is also high. When defatted soybeans are treated with an extruder, antigenicity can be lowered, but NDIPC cannot be lowered. On the other hand, in the feed material of the present invention obtained by adding 7 to 24% by weight of saccharide to the total of defatted soybean and saccharide and heating and pressure kneading, the antigenicity is 100 U / 10 mg or less, particularly It can be reduced to 50 U / 10 mg or less, and NDIPC can be reduced to 30% or less. The antigenicity can be measured by an ELISA method known to those skilled in the art.

  The feed raw material of the present invention obtained as described above may be fed as it is to the animal, or may be fed to the animal as a feed blended with a feed raw material known in the art. Such feed materials include grains such as rice, brown rice, rye, wheat, barley, corn, and milo; bran and defatted rice bran; corn gluten meal, corn germ meal, corn gluten feed, corn steep liquor, etc. Manufacturing oils: Vegetable oils such as soybean oil, rapeseed oil, sweet candy, coconut oil, oils such as soybean oil, refined beef tallow, animal oil; magnesium sulfate, iron sulfate, copper sulfate, zinc sulfate Inorganic salts such as potassium iodide, cobalt sulfate, calcium carbonate, tricalcium phosphate, sodium chloride, calcium phosphate, choline chloride; amino acids such as lysine, methionine; vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin B12 , Vitamin D3, vitamin E, calcium pantothenate, di Chin acid amides, vitamins such as folic acid; forage; fish meal, skim milk powder, animal matter feeds such as dried whey, such as hay and the like.

  The amount of the low antigenic and highly digestible feed raw material of the present invention when blended with other feed raw materials is 1 to 50% by weight as the feed raw material, and the amount of sugar added to the defatted soybean derived from the feed raw material. It may be 0.07 to 12% by weight, preferably 0.5 to 10% by weight. The low antigenicity and highly digestible feed material of the present invention can provide a palatability improving effect in a very small amount.

  The low antigenic and highly digestible feed material of the present invention and the feed containing the same are processed into shapes such as powders, mashes, granules, flakes, pellets, briquettes, etc., as long as they are solid. The

  Examples of the animal that provides the low antigenic and highly digestible feed material of the present invention and the feed containing the feed material include animals such as pigs, cows, chickens, sheep, goats, dogs and cats. In particular, it is suitable for animals in the infancy period (for example, weaning period to substitutional milk period in the case of pigs).

Hereinafter, the present invention will be described in detail by way of examples. However, the present invention is not limited to the following examples.
[Examples 1 to 12] Production of feed material with low antigenicity and high digestibility (1)
In order to improve the digestibility and palatability of the biaxial extruder-treated defatted soybeans, a test was performed in which about 50% sugar solution was added from the hydration line to the two types of defatted soybeans during the biaxial extruder treatment. Table 1 shows the properties of the raw materials used. FIG. 1 illustrates the configuration of a biaxial extruder (KEI-87, Kowa Kogyo, die: φ2.5 mm × 226 holes) (NL × 2, NR × 4, FFFPFFFFFFF from the tip).

  The amount of PF-S added was changed as shown in Table 2 to confirm how the degree of processing (water content, crude protein content and antigen content) changed.

The following ELISA method was used for the measurement of the antigen amount. The standard antigen solution was diluted 100-fold with 10 mM phosphate buffer and immobilized on a microtiter plate by a conventional method. To this plate, a standard solution of each diluted solution, or a sample solution extracted from a test substance with a phosphate buffer and a calf antiserum were added, and an antigen-antibody reaction was performed. Then, it was washed with 10 mM PBS containing 0.05% Tween80. A peroxidase-labeled anti-calf antibody (Code P-159, manufactured by DAKO) was added as a secondary antibody to carry out the reaction. This was washed with the above washing solution, and 8 mM o-phenylenediamine containing 0.008% H ₂ O ₂ was added to cause color development. After about 30 minutes, the reaction was stopped with 100 ml of 4N H ₂ SO _{4 and} the absorbance at 492 nm was measured with a microplate reader. The antigen value of the standard antigen solution in this measurement was 10,000 U / 10 mg, which was a unit indicating the content of the antigen substance. The log dilution ratio of the standard antigen solution and the absorbance logit conversion value were taken on the X and Y axes to obtain a standard straight line. A sample was prepared from the feed raw material obtained by the heat and pressure kneading process in the same manner as the standard antigen solution was processed, and the antigenicity was calculated from the standard line. The absorbance logit conversion value is expressed by the following formula:
logit = ln [(ABS / cont.ABS) / (1-ABS / cont.ABS)]
[ABS indicates the absorbance at the time of addition of the standard antigen solution at each dilution, cont. ABS indicates the absorbance when 10 mM phosphate buffer is added.]

1) Product temperature in the cylinder: near the outlet 2) Product temperature in the cylinder: near the middle

  In the vicinity of 24% where the PF-S addition rate is the lowest (sugar content 13%), a blowing sound is generated at the die outlet as the pressure decreases, and about 14% (sugar content about 7%) is the lower limit of addition. became. In addition, when PF-S is added in an amount of 30% or more (carbohydrate content of about 17%), coloring tends to decrease and the amount of antigen tends to increase, and about 40% (carbohydrate content of about 24%) is the upper limit of addition. It became. These were the same for both LP defatted soybeans and HP defatted soybeans. From the above test results, it is determined that the PF-S addition rate is about 14 to 40%, that is, the carbohydrate content is about 7 to 24% by weight. In actual production, the PF-S addition rate is slightly over 26% (the carbohydrate content). 12-17% by weight) was determined to be reasonable.

  FIG. 2 shows the results when the same treatment as in Examples 1 to 12 was carried out with the PF-S addition rate changed, in order to see the change in NDIPC (%) with respect to the PF-S addition rate by the heat and pressure kneading treatment. Indicates. In the figure, the solid line shows the result of autoclaving at 120 ° C. for 30 minutes by adding a 50% aqueous solution of glucose to defatted soybean to reduce the antigen content to 100 U / 10 mg or less to a sugar content of 10%. Indicates NDIPC. When the defatted soybean is autoclaved under the same conditions without adding sugar, the antigen amount is as high as 130 U / 10 mg, and NDIPC is 16.7%. In the figure, the NDIP at the solid line is about 36%, and even if the amount of added saccharide is increased further, the value of NDIP hardly changes. The same value was obtained when xylose was added instead of glucose. When sucrose was added, the same processing temperature was set to 130 ° C. in order to reduce the amount of antigen to 100 U / 10 mg or less, but NDIP would similarly exceed 30%. In addition, in the commercially available sweetened heated soybean meal (sugar addition amount of about 2%), the antigen amount is as high as 150 U / 10 mg, and NDIPC is about 24%. From FIG. 2, it can be seen that in the heat and pressure kneading process, the PF-S addition rate increases and NDIPC decreases. In this case, the amount of antigen was 50 U / 10 mg or less. In particular, when the addition rate of PF-S is 24% or more (carbohydrate content 13%) or more, NDIPC is lowered to 23% or less. When a linear approximation line is obtained from these plots, it can be seen that NDCP starts to decrease when the PF-S addition rate is 15%, that is, the carbohydrate addition rate is about 7.9%.

  In FIG. 3, the change of the pepsin digestibility (%) with respect to PF-S addition rate is shown. In the figure, the solid line indicates the pepsin digestibility when no carbohydrate is added. FIG. 3 shows that when the PF-S addition rate is in the range of 22-30%, the pepsin digestion rate is 94.2% -95.4%, which is higher than that without addition. When a linear approximation straight line is obtained from this plot, it can be read that the pepsin digestibility increases when the PF-S addition rate is 15%, that is, the carbohydrate addition rate is about 7.9%.

[Example 13] Production of feed material with low antigenicity and high digestibility (2)
In order to improve the digestibility and palatability of the defatted soybeans treated with biaxial extruder, it is commercially available from hydration line to LP defatted soybeans during treatment with biaxial extruder (KEI-45, die: φ5mm × 2 holes). A test was conducted in which the molasses for feed was diluted 2 times with water and added.

  The antigen content of the processed product obtained in this test was 16.5 U / 10 mg, NDIPC was 18.6%, and pepsin digestibility was 94.8%.

[Examples 14 to 16] Mouse preference test 12 male ICR mice (6 mice x 2 test section) 4 weeks old were purchased from Charles River Japan (closed, Crlj: CD1 (ICR)), and each cage was tested. One mouse was allocated, two types of feed were placed in each cage, feed was freely selected and consumed in the cafeteria system, the amount of each feed intake was measured, and palatability due to differences was judged. First, pre-breeding was taken for 4 days to make the mouse accustomed to the environment. In preliminary breeding, CE-2 pellets (manufactured by CLEA Japan, Inc.) were given. The test breeding which gave the feed of the mixing | blending shown in Table 4 to the mouse | mouth which became 5 weeks old at the start of a use test was performed for 5 days. As a measurement item, feed intake was measured every day at 10:00 am 1 to 5 days after the start of the test. The results are shown in FIGS.

* Basic feed: CE-7 powder (manufactured by CLEA Japan)
** Invention product: HP defatted soybean with 29% by weight of saccharide solution (product name: PF-S, manufactured by Calpis Co., Ltd.), heated and pressurized under conditions of maximum product temperature of 160 ° C and maximum pressure of 10 kg / cm ² What was obtained by kneading

  In Test 1 (Example 14 and Comparative Example 1) in FIG. 4 and Test 2 (Example 15 and Comparative Example 2) in FIG. 5, there is little difference in food intake due to the effect of adding PF-S on the first day. The difference became remarkable after the first. Although the product of the present invention also has an attracting effect, it is considered that a taste improving effect is obtained by improving the taste beyond that.

  In Test 3 of FIG. 6 (Example 16 and Comparative Example 3), Example 16 (basic feed + product of the present invention) is more effective than Comparative Example 3 (basic feed + biaxial extruder treated defatted soybean + sugar + defatted milk powder). It was confirmed that the palatability was significantly high. This suggests that these raw materials are not simply mixed, but that lactic acid fermentation and heating and pressure kneading in the presence of a saccharide solution were factors that increased palatability. It is.

  Regarding the blending amount, the PF-S concentration in the final feed was about 6% in Example 14 (sugar content about 3.3%), and about 15% in Example 15 (sugar content about 1.7%). ) Thus, it became clear that the palatability improving effect was obtained with a very small amount.

[Example 17] Pork palatability test The product of the present invention (HP defatted soybean, 30% by weight of a carbohydrate solution (product name: PF-S, manufactured by Calpis Co., Ltd.)) is added to a feed for suckling piglets, and the maximum product temperature is 250 ° C. X The one obtained by adding heat and pressure kneading under the condition of the maximum pressure of 16 kg / cm ² was examined.

  8 days old LW / D seed pigs (4 castrations, 4 females each) were tested for 4 days after introduction, and mixed with equal amounts of control feed and 10% mixed feed of the present invention. We paid and pre-bred and confirmed that there was no abnormality in health condition.

  The palatability test was divided into three periods (18 days in total) with one period being 6 days. In each period, the test feed containing the present invention product by substituting the non-test sample-added control feed (Table 5 shows the mixing ratio of the basic feed and Table 6 shows the component composition) and skim milk powder in the control feed A feed was prepared. The blending ratio of the product of the present invention was 10% in the first period, 20% in the second period, and 35% in the third period.

1) In 1 kg; 1 g of thiamine nitrate, 7 g of riboflavin, 0.5 g of pyridoxine hydrochloride, 6 g of nicotinic acid amide, 10.9 g of calcium D-pantothenate, 57.6 g of choline chloride
2) In 1 g; Vitamin A oil 10,000 IU, vitamin D3 2,000 IU, dl-α-tocopherol acetate 10 mg
3) In 1 kg; Mn 50 g, Fe 50 g, Cu 10 g, Zn 50 g, I 1 g
4) Calculated values based on the Japanese standard feed ingredient table (2001 version) 5) Satisfaction rate with respect to the requirement for inclusion in the Japanese feeding standard / pig (2005 version)

  Each test pig is housed individually in a stainless steel cage (floor surface 1.2 x 1.2 m, soft plastic-coated mesh floor) with two feeders in the floor warming windless barn. A control feed was put into the vessel, and a test feed was put into the other feeder once a day at 600 to 1,200 g, and allowed to be freely selected for 6 days in each period. In order to remove the influence of the feeder position, the type of feed to be introduced was changed every day, and the feed was updated to a new feed every day. There was no habituation period in each period, and the lighting period was 15 hours in each period. Drinking water was a constant salary. The temperature in the barn during the test period was a minimum of 15 ° C and a maximum of 29 ° C.

The survey items during the test period are as follows.
1) Body weight Individual body weights were measured on the start date and the end date of each period (the end date of the first period and the start date of the second period, and the end date of the second period and the start date of the third period were the same day).
2) Feed intake Every day, the remaining feed was measured at a certain time to calculate the feed intake, and the intake ratio of the control feed and the test feed was calculated.
3) Health condition Daily health condition (general condition and occurrence of diarrhea) was observed individually in the morning and evening twice. The occurrence of diarrhea was classified into normal, soft stool, mud stool and chickenpox stool.

  The intake ratio of the control feed and the product of the present invention in each period was converted into angles, and then analyzed for each period by a two-way method based on the position of the feed and feeder, and the difference between the mean values was significant. (Reference: Experimental design method centering on livestock, Yokendo). Table 7 shows the feed intake ratio in each period.

1) Average value of feed intake ratio of 8 animals for 6 days ± standard deviation 2) (inside parentheses are standard errors) 3) ANOVA is performed by one-sided test 4) *; there is significant difference 5) Test feed Product mix ratio: 1st period 10%, 2nd period 20%, 3rd period 35%

  At any time, the intake ratio of the product of the present invention tended to be higher than that of the control feed. There was a significant difference between the first and second stages. There was no significant difference in the feeding position and the interaction between the feeding feed and the feeding position at any time.

  There was a tendency for the difference in the intake ratio between the product of the present invention and the control feed to decrease with the passage of time, and there was a concern about the effects of habituation to the feed of the test pigs. The cause could not be clarified because the blending ratio of the product was increased.

Table 8 shows changes in body weight and the occurrence of diarrhea during the test period.
Note) A total of 6 days in each period, with 1 day divided into 2 time points (morning and evening)

  As shown in Table 8, in the first period, except for one animal, excretion of soft stool and mud stool was observed, but the degree of diarrhea decreased with the passage of days after the start of the test. In stage 3, only loose stool was observed, and the frequency of occurrence decreased in most individuals. In addition, in any individual, no abnormality is observed in the health state, and it is considered that there is almost no influence on the feed intake by the health state. In addition, in the test in the third period, the weight growth was compared between 3 individuals who ate more of the control feed and 5 individuals who ate more of the product of the present invention, and no significant difference was observed. The product was considered to show the same nutritional value as the control feed.

  As described above, the test feed at any time tended to have a higher intake than the control feed. In particular, a significant difference was observed in the first and second stages. At any time, there was no significant difference in the feed feeding position and the interaction between the feed type and feeding position.

Claims (8)

  A saccharide solution is added to defatted soybean so as to have a saccharide content of 7 to 24% by weight, and heat and pressure kneading treatment is used. Antigenicity is 100 U / 10 mg or less, and high digestibility is neutral. A feed raw material characterized by being 30% or less as a detergent-insoluble protein.   The feed material according to claim 1, wherein the sugar solution is fermented milk or fermented milk-containing material.   A method for producing a feed material, comprising adding a saccharide solution to defatted soybean so as to have a saccharide content of 7 to 24% by weight, followed by heating and pressure kneading.   The method for producing a feed raw material according to claim 3, wherein the sugar solution is fermented milk or fermented milk-containing material.   The method for producing a feed raw material according to claim 3 or 4, wherein the carbohydrate content of the carbohydrate solution is 30 to 70% by weight.   The feed which mix | blended the feed raw material of Claim 1 or 2 so that the carbohydrate content derived from this feed raw material might be 0.07 to 12 weight%.   A saccharide solution added to defatted soybean so that the saccharide content is 7 to 24% by weight, and the heat and pressure kneading treatment after the addition reduces the antigenicity to 100 U / 10 mg or less, and the high digestibility is neutral. The said carbohydrate solution for manufacturing the feed raw material used as 30% or less as a detergent insoluble protein.   The saccharide solution according to claim 7, wherein the saccharide solution is fermented milk or fermented milk-containing material.