JP2654889B2 - Raw material for feed for young animals - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a raw material for feed of young animals in which the content of antigenic substances (soy allergen) in soybeans or processed soybean products is adjusted so as not to cause allergic symptoms such as diarrhea.

[0002] The term "soybean processed product" as used herein means a product obtained by treating soybeans by a physical and / or chemical method, and examples thereof include a crushed full-fat soybean product, a defatted soybean product, and a soybean protein extract.

[0003]

2. Description of the Related Art The above-mentioned soybeans or processed soybean products have been conventionally used as raw materials for livestock compound feed as a source of high-quality and inexpensive protein.

[0004] However, when a soybean or a processed feed containing processed soybean products as a main raw material of a protein is given to an animal of a young age, allergen contained in the soybean or processed soybean product causes intestinal villus atrophy and diarrhea. It has been known that allergic symptoms such as this cause poor growth and affect the subsequent growth stage. This is a big problem for producers. Therefore, conventionally,
In childhood, expensive skim milk powder is used as a protein raw material, and a method of switching to soybeans or processed soybeans when the digestion and biological defense mechanisms are completed is generally bred.

[0005] Even when skim milk powder is used, stagnation of growth or weight loss is observed in the transition to feed using vegetable protein. Therefore, by gradually increasing the ratio of plant protein raw materials that are unlikely to cause allergies as soon as possible, it is possible to suppress stagnation of growth at the time of shifting to plant protein feed, and it is possible to fatten animals smoothly.

[0006] The limited use of soy or processed soy products in young animals is due to the allergen content in the soy or processed soy products. Normally, defatted soybean meal produced in the oil production process contains a large amount of allergens ranging from 1.000 to 10,000 units. Therefore, it has been proposed to remove allergens.

As a method for removing allergens in soybeans or processed soybean products, there is a method of heating and treating with alcohol (Japanese Patent Application Laid-Open No. 60-164441). This method is 1) high in cost because 3 to 4 times the amount of lower alcohol is added to soybeans or processed soybeans and heat-treated; 2) large-scale equipment is required; There is a drawback that the property deteriorates. In addition, the amount of residual allergen by this treatment method is several hundred units, and it cannot be said that allergen removal is sufficient.

As a method for removing allergens, a method of heat treatment is also known. In this method, about the same weight of water is added to a processed soybean product and heated at a temperature of 100 to 140 ° C. for about 60 minutes. The treatment is disadvantageous in that it is not preferable in terms of cost and equipment, and that other components may be denatured due to heating for a long time.

[0009] Apart from the above treatment, an expansion method is used as a method for treating soybeans or processed soybean products at high temperature and high pressure.
The extruder method is known. However, these treatment methods are merely intended to utilize the improvement of physical properties (for example, improvement of digestibility by organizing), and when used as a raw material for compound feed, the purpose is to improve the appearance or digestibility. The processed product is used only as a general raw material for compound feed.

[0010]

As described above, there is a processing method aimed at reducing allergens, but there are problems with the amount of residual allergen and processing cost. On the other hand, there are an expansion method and an extruder method as high-temperature and high-pressure treatment methods, but they have not been studied in relation to the amount of allergen.

An object of the present invention is to provide a soybean processing raw material for a compound feed that is effective for young animals that cause allergic symptoms due to allergens.

The present inventors have studied the allergen removal treatment method and the resulting change in the amount of allergen and the allergic symptoms of the treated animals, and as a result, the soybean material was treated by a specific high-temperature, high-pressure treatment method. 100
The present invention has been completed by finding that the following unit or less can be used as a raw material for infant animal feed.

[0013]

The raw material for feed of young animals of the present invention is characterized in that the content of allergen is adjusted to 100 units or less by subjecting soybeans or processed soybean products to high temperature and high pressure. An important point in the present invention is that the allergen in the protein for feed for young animals is set to 100 units or less. In general, diarrhea onset in young animals decreases as the allergen content in the feed decreases,
Removing allergens from soy-based feed requires harsh treatment and is undesirable from an economic and nutritional perspective. The present inventors impair the nutritional value, palatability, etc. of feed by reducing the allergen content of soybeans and processed soybean-derived feed ingredients to 100 units or less by short-time treatment at high temperature and high pressure. Not
It was found that the same effect as eliminating allergen was obtained, that is, the effect that the young animals did not cause diarrhea at all was obtained.

The measurement of the amount of allergen (antigen substance) in the present invention was carried out by an ELISA method. That is, a standard antigen solution (a solution obtained by extracting defatted soybean powder obtained by crushing soybeans with n-hexane, drying and then pulverizing the defatted soybean powder with a 5-fold amount of a pH 7.2 phosphate buffer, and then filtering) was used. Was diluted in a conventional manner and immobilized on a synthetic resin plate (Nunc-lmmuno Plates 11, Nippon Intermed Co., Ltd.), and 50 μl of a sample antigen solution prepared by the same treatment as the standard antigen solution was added thereto. After adding 50 μl of an antibody solution obtained by diluting calf serum to 1/1000, a competition reaction was performed at room temperature for 30 minutes. In addition, the serum was prepared by giving a milk substitute containing defatted soybean to a calf soon after birth, and collecting blood from an individual in which soybean allergy was induced. After the reaction, the antibody that did not bind to the immobilized antigen and the free sample antigen were washed away, and a 1/500 diluted peroxidase-labeled anti-calf antibody (DAKO Code P-159) was used.
100 µl was added and reacted at 37 ° C for 30 minutes. After washing away the free anti-calf antibody, add 0.008% hydrogen peroxide and 8 mM o-
100 μl of a phenylenediamine mixed solution was added, the color was developed at room temperature for 30 minutes, the reaction was stopped with 100 μl of 4N sulfuric acid, and the absorbance at 492 nm was measured.

In this measurement, the unit indicating the content of the antigen substance is that the antigen value of the standard antigen solution is 10,000 U, and the value of each antigen sample is the log dilution ratio and the absorbance lo of the standard antigen solution in FIG.
The git conversion value was determined from a standard straight line on the X and Y axes. The converted absorbance logit value shown in FIG. 1 is obtained by the following equation. logit = ln [(ABS / cont.ABS) / (1-ABS / cont.ABS)] where ABS is the chromogenicity at the time of addition of standard antigen solution at each dilution cont.ABS is pH 7.2 with phosphate buffer Table 1 shows the allergen content of various soybean-related products according to this measurement method.

[0016]

As a high-temperature and high-pressure treatment method, a twin-screw extruder treatment is preferred from the viewpoints of cost and equipment and that various conditions can be set. Conventionally, soybeans or processed soybean products have been treated with an extruder, particularly a twin-screw extruder, but there have been no examples examined in relation to allergens. in this case,
As the allergen is reduced, the digestibility is naturally improved. Unlike alcohol treatment, the palatability is not impaired. In addition, since heating is not performed for a long time as in the conventional method of reducing allergen by heat treatment, denaturation such as oxidation does not occur,
Preference is not impaired.

In order to reduce the amount of allergen to 100 units or less by the heating and pressurizing treatment of the present invention, for example, the soybean or processed soybean product (such as defatted soybean) is not subjected to any pretreatment or the like, and the biaxial type extract is directly fed from the feeder. It is possible to do this by injecting water from a feeder or another inlet at the same time as charging into a ruder and subjecting it to high-pressure heat treatment. The amount of water is preferably 5 to 50% (weight ratio) with respect to the raw materials. The input material is barreled at a barrel temperature of 1 with a twin-screw extruder.
00 ~ 300 ℃, preferably 120 ~ 220 ℃, pressure 10 ~ 100kg
/ Cm ² , after heating and pressurizing for a short time under the set conditions of screw rotation of 200 to 300 rpm, extruding into the atmosphere to expand and denature, then cutting this to about 2 to 10 mm, and moisture of 12% or less (preferably (10% or less) and pulverize as necessary to obtain the desired feed material. Depending on the type of soybean or processed soybean product to be treated and the amount of allergen, etc., the residence time of the barrel portion is usually 10 to
It is about 180 seconds, preferably about 30-60 seconds. By selecting a barrel temperature, a pressing force, a raw material input amount, and a screw rotation speed in accordance with an object to be processed, an allergen reduction amount can be arbitrarily adjusted.

[0019]

The present invention will be described below with reference to examples. In Examples,% indicates% by weight. Example 1 A defatted soybean for feed was treated with a twin-screw extruder (KE145-25, manufactured by Kowa Kogyo Co., Ltd.) at a barrel temperature of 180.
° C, pressure 45kg / cm ² , rotation speed 280rpm, raw material supply 77kg
/ Hr, die 2 mm × 10 holes, and extruded into the atmosphere to expand and denature. The treated material was cut into a size of about 3 to 5 mm, dried sufficiently, and then pulverized to obtain a feed material.
Treatment conditions, allergen analysis results, and the onset of diarrhea when 2-week-old calves were administered a diet containing 20% of the protein raw material obtained in this example for 1 week and then a diet containing 60% of the protein for 3 weeks The presence or absence is shown in Table 2.

[0020]

Example 2 Using the same raw materials and the same machine as in Example 1, the barrel temperature was 140
° C, pressure 54kg / cm ² , rotation speed 280rpm, raw material supply 77kg / h
r, treated under the condition of a die of 2 mm × 10 holes, extruded into the atmosphere, and expanded and denatured. This treated material was cut into a size of about 3 to 5 mm, dried sufficiently, and then pulverized to obtain a desired feed material. Table 2 shows the treatment conditions, allergen analysis results, and the presence or absence of diarrhea when a 2-week-old calf was administered a diet containing 20% of the protein for 1 week and then a 60% diet for 3 weeks. The feed raw materials obtained in Examples 1 and 2 did not show diarrhea even at a high allergen amount and showed poor growth, as compared with those obtained by reducing the allergen by the conventional alcohol treatment method and the heat treatment method. Did not. These results are presumed to be due to the fact that the conventional treatment method reduces allergens and also removes components that suppress the onset of allergic symptoms contained in soybeans and the like. In addition, compared to the conventional treatments, the bait was better and good digestibility and growth were observed.

[0022]

According to the present invention, a processed soybean product is subjected to high-temperature and high-pressure treatment in a short time by a twin-screw extruder,
It is a low-cost, low-volume facility that is obtained by reducing the amount of allergens to 100 units or less, does not cause diarrhea, has a high digestibility, and does not reduce palatability and nutritional value. It is suitable as a raw material for infant animal feed.

[Brief description of the drawings]

BRIEF DESCRIPTION OF DRAWINGS FIG. 1 is a graph showing an allergen standard line of ELISA absorbance.

 ──────────────────────────────────────────────────続 き Continuation of front page (72) Inventor Akio Oishi 1-7-40 Oiwake, Shimizu-shi, Shizuoka (56) References JP-A-60-251850 (JP, A) JP-A-62-224251 (JP, A) )

Claims (2)

(57) [Claims] Claims 1. A soybean or a processed soybean product ,
The water content is 5 to 50% by weight, and the barrel temperature is 100 to 30%.
The residence time of the barrel at 0 ° C. is 10 to 180 seconds.
High temperature and high pressure treatment by shaft extruder
Allergen content measured by ELISA
Raw material for feed for young animals adjusted to have an amount of 100 units or less . 2. A soybean or a processed soybean product is supplied to a twin-screw extruder together with 5 to 50% by weight of water, a barrel temperature is 100 to 300 ° C., a residence time of the barrel portion is 10 to 180 seconds, and a pressure is 10%. １００100 kg / cm ² and allergens measured by ELISA
A feed for a young animal feed comprising reducing the allergen content so that the content is 100 units or less .
Manufacturing method .