JP5347121B2 - Device for collecting / dispersing magnetic particles in liquid - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an apparatus for separating and dispersing magnetic particles and a liquid at high efficiency by moving magnetic means so that the magnetic particles suspended in the liquid in a container are collected in a certain position. <P>SOLUTION: The apparatus for collecting/dispersing magnetic particles 11 in a liquid 10 uses movement means to move magnetic means 4 on the side of a container 1. The container 1 is made of either a tube, a chip, a microplate or a PCR plate. The movement means includes a motor provided on a side of a casing having a large number of containers 1 retained in respective retaining holes, the motor being controlled by a controller, a pinion provided in the motor, a rack vertically movably provided on the side of the casing and meshing with the pinion, and a rack guide provided in the casing for vertically movably guiding the rack. The rack guide includes a rack connection having a C-shaped sliding element having the rack and taking the form of C, and a guide provided on the casing and engaging with the C-shaped sliding element. <P>COPYRIGHT: (C)2012,JPO&amp;INPIT

Description

  The present invention relates to an apparatus for assembling / dispersing magnetic particles in a liquid, and in particular, magnetic particles mixed and suspended in a liquid in a container are aggregated at an arbitrary position by moving magnetic means, thereby magnetic particles The present invention relates to a novel improvement for efficiently separating and dispersing liquids and liquids.

For example, the configuration disclosed in Patent Documents 1 and 2 can be shown in FIG. 18 as an apparatus for dispersing and dispersing magnetic particles of a DNA protein screening apparatus of this type that has been conventionally used.
That is, in the protein screening apparatus disclosed in Patent Document 1 described above, in order to extract protein from the liquid to be screened contained in the container 1, the binding step A, the washing step B and the extraction shown in FIG. A screening process consisting of process C is employed.

  First, in the binding step A, a protein or chemical substance, which is a reagent sample, and magnetic fine particles are injected into a container 1 through a piston pump 3 with a tip 2 which is a suction means, stirred, and then the bottom of the container 1 By providing the magnet 4 as a magnetic means, the magnetic fine particles are gathered at the bottom of the container 1 to perform solid-liquid separation by magnetism.

Next, after the supernatant in the solid-liquid separated container 1 is sucked with the chip 2 and taken out to the outside, the cleaning liquid is put into the container 1 using the piston pump 3 in the cleaning step B, and the chip 2 is placed in the container 1. Stirring is performed by inserting into the tube and repelling (pipetting) suction and discharge.
Thereafter, similarly to the coupling step A, the magnet 4 is provided at the bottom of the container 1 to perform solid-liquid separation by magnetism, and the cleaning is completed by sucking and discarding the supernatant.

Next, in the extraction step C, an extraction liquid, which is a dissociation reagent, is injected into the container 1 and separated by stirring by pipetting by suction and discharge of the chip 2. By providing the magnet 4 at the bottom, magnetic solid-liquid separation is performed.
In this state, since the supernatant contains a protein or chemical sample, the extracted sample can be recovered by aspirating and removing the supernatant with the chip 2.

JP 2006-194671 A JP 2001-70827 A

Since the conventional apparatus for collecting / dispersing magnetic particles in a liquid is configured as described above, the following problems exist.
That is, in the case of solid-liquid separation in which the liquid is sucked in each of the processes A, B, and C using the magnetic means described above, if there is a magnetic particle near the suction port of the chip that sucks the liquid, it may be mistakenly sucked. Since the property is high, the position for collecting the magnetic particles is preferably near the liquid surface as far as possible from the suction port of the chip.
On the other hand, when the magnetic particles are dispersed using the water flow generated by the suction and discharge of the liquid by the piston pump provided on the tip in the separation by stirring, the position of the magnetic particles collected near the liquid surface far from the tip suction port is remarkably increased. There is a concern that the water flow is attenuated and the dispersion time is prolonged. Therefore, it is desirable that the magnetic particles are collected near the tip suction port where the liquid flow is fast. As described above, the ideal magnetic particle recovery position differs between “solid-liquid separation” and “dispersion”. Therefore, the magnetic position can be moved arbitrarily to change the recovery position, and the recovered magnetic particles can be guided to an arbitrary position. If a possible structure and process can be added, the efficiency of the process can be improved and the magnetic position can be moved to an appropriate position even with respect to changes in the liquid volume, but the magnetic means is attached to the bottom of the container. However, it is difficult to reduce the solid-liquid separation and dispersion time because the gathering position of the magnetic particles in the container by magnetic attraction cannot be changed according to various purposes.

An apparatus for collecting / dispersing magnetic particles in a liquid according to the present invention mixes and disperses magnetic particles in a liquid contained in a container, and disperses the magnetic particles in the container using magnetic means from the outside of the container. By moving to a predetermined position, the liquid can be sucked at least using a suction means;
In the apparatus for collecting / dispersing magnetic particles in a liquid, wherein the magnetic means is moved with respect to the container via a moving means, and the magnetic particles are collected at an arbitrary position in the container. permanent magnet by Rinari, wherein the magnetic means by the moving means so as to move the sides of the container, the container is a tube, made more either chip, said moving means, a number of containers each a motor controlled by the controller provided on the side of the casing having been held in the holding hole, a pinion provided on the motor, and the housing front Symbol pinion located vertically movably on the side of A rack to be engaged , a rack coupling body (27) having the rack (25), a C-shaped sliding body (28) provided in the rack coupling body (27) and having a C-shape as viewed from the longitudinal direction, The C-shape provided on the side part (22a) of the housing (22) A guide body (29) engaged with the sliding body (28), and a holding body (30) connected to the rack (25) via a connecting member (25a) and having the magnetic means (4) at the tip. The holding body (30) is arranged in parallel in the same direction as the longitudinal direction of the rack (25), and penetrates the through hole (31) of the housing (22) to form the housing (22). protrude within the permanent magnet of the magnetic means is configured ing a cylindrical or square pillars.

Since the apparatus for collecting / dispersing magnetic particles in a liquid according to the present invention is configured as described above, the following effects can be obtained.
That is, since the corresponding position of the magnetic means in the container can be arbitrarily changed via the moving means, the magnetic means can be moved to an optimum position for the solid-liquid separation and dispersion process, and the solid-liquid separation The dispersion process can be shortened. At the same time, since it is possible to follow the change in the liquid amount, the time of the process, which was a problem when using magnetic particles having a small particle size, can be shortened. Further, according to the present invention, since the permanent magnet is cylindrical, it is one of the corner posts, it is possible to perform collection / distribution of the magnetic particles in any vessel.

1 is a configuration diagram showing an apparatus for collecting / dispersing magnetic particles in a liquid according to the present invention. 1 is a configuration diagram showing an apparatus for collecting / dispersing magnetic particles in a liquid according to the present invention. FIG. 3 is a specific configuration diagram illustrating an aggregation / distribution apparatus according to the present invention. FIG. 4 is a cross-sectional view of FIG. 3. It is a block diagram which shows the magnetic means with respect to a container. It is a block diagram which shows the other form of FIG. It is a block diagram which shows the other form of FIG. It is a block diagram which shows the aggregate | assembly of a magnetic particle. It is a block diagram which shows re-dispersion from solid-liquid separation. It is process drawing which shows the conventional coupling | bonding, washing | cleaning, and extraction process.

  The present invention is a liquid in which magnetic particles mixed and suspended in a liquid in a container are gathered at an arbitrary position by moving a magnetic means, and separation and dispersion of magnetic particles and liquid are performed with high efficiency. It is an object to provide an apparatus for collecting / dispersing magnetic particles therein.

Hereinafter, preferred embodiments of an apparatus for collecting / dispersing magnetic particles in a liquid according to the present invention will be described with reference to the drawings.
In addition, the same code | symbol is attached | subjected and demonstrated to a part the same as that of a prior art example, or an equivalent part.
FIG. 1 shows an ideal position of magnetic particles in the solid-liquid separation process according to the present invention (that is, gathering to a specific position in the container 1), and magnetic means (near the upper surface of the liquid 10 in the container 1). permanent magnet or Ranaru) 4 is moved and the tip of the suction port 2a of the suction means 2 consisting of chip is positioned on the bottom of the container 1, without sucking the magnetic particles 11, the efficiency only the liquid 10 in a short time It can be sucked well and only the magnetic particles 11 can remain in the container 1.

FIG. 2 shows the ideal magnetic particle collection position (that is, collection at a specific position in the container 1) in the dispersion process by moving the magnetic means 4 to the bottom side of the container 1.
Therefore, at the position of FIG. 2, the magnetic particles 11 are gathered to the bottom side of the container 1 and the suction port 2 a at the tip of the suction means 2 is located in the vicinity of the magnetic particles 11. By reversing the suction and discharge of 10 (pipetting), dispersion by stirring of the liquid 10 and the magnetic particles 11 can be achieved quickly in a short time.

As shown in FIGS. 1 and 2, the magnetic means 4 is moved along the side of the container 1, for example, along its longitudinal direction, as shown in FIGS. A moving means 20 is used.
The moving means 20 includes a motor 23 which is controlled a large number of containers 1 in the controller C is provided on the side portion 22a of the housing 22 having been held in each holding hole 21, a pinion 24 provided on the motor 23 When a rack 25 which meshes with the previous SL pinion 24 located vertically movably on the side portion of the housing 22, the side 22a side of the housing 2 2 for guiding the rack 25 vertically movable a rack guide body 26 located more becomes, the rack guide body 26, rack coupling body 27 having a C-shaped slide 28 forming a C-shape as viewed from the longitudinal direction of the rack 25 Yes perilla A guide body 29 provided on a side portion 22a of the housing 22 and engaged with the C-shaped sliding body 28, and connected to the rack 25, which will be described later, via a connecting member 25a, and the magnetic means 4 at the tip. a holding member 30 having a pressurized et al It has been made.

The rack 25 to the connecting member 25a above the coercive bearing member 30 which is connected through the in the longitudinal direction while being arranged in parallel in the same direction and the rack 25, through the through hole 31 of the housing 22 A magnetic means 4 made of a permanent magnet is provided at the tip of the holding body 30 so as to protrude into the housing 22.

By rotating the motor 23, the holding body 30 moves up and down via the pinion 24 and the rack 25, so that the magnetic means 4 is moved in the direction of arrow A, and is moved to the side of the container 1 provided in the housing 22. On the other hand, the magnetic means 4 can be arbitrarily moved to the position shown in FIG. The moving position of the rack coupling body 27 is configured to be detected by a position sensor 40 that performs well-known optical or magnetic detection.

Further, FIG. 5 or et Figure 7 shows another embodiment of the magnetic means 4, 5, Iteiru use permanent magnets of cylindrical or prismatic with respect to the container 1 consisting of a tube.
FIG. 6 shows a case where a cylindrical or prismatic permanent magnet is used for the tubular container 1.
FIG. 7 shows a configuration in which the magnetic means 4 using a permanent magnet made of a cylinder or a prism is moved up and down on the side of the container 1 made of a known chip.

FIG. 8 shows a state in which the magnetic means 4 located on the side of the container 1 is located near the lower part of the container 1 and the magnetic particles 11 are gathered to the lower part. The magnetic means 4 is moved from the A position to the upper B position. This shows a state in which the magnetic particles 11 gather at the position B near the liquid surface.

FIG. 9 shows a state of changing from the solid-liquid separation step to the redispersion step.
That is, in the solid-liquid separation step, since the magnetic means 4 moves to the upper B position, the magnetic particles 11 are moved upward, so that the liquid can be sucked by the chip 2 from the lower part. By lowering to the C position, the magnetic particles 11 are gathered and guided to the lower part of the container 1, and by further retracting the magnetic means 11 to a position further away from the lower part of the container 1, the magnetic particles 11 in the liquid 10 are recovered. Changes from a collective state to a distributed state.

1 container 2 suction means (chip)
2a Suction port 3 Piston pump 4 Magnetic means 10 Liquid 11 Magnetic particles 20 Moving means 21 Holding hole 22 Housing 23 Motor 24 Pinion 25 Rack 25a Connection member 26 Rack guide body 27 Rack coupling body 28 C-shaped slide body 29 Guide body 30 Holding body 30a Mounting hole 31 Through hole C Controller 40 Position sensor

Claims (1)

The magnetic particles (11) are mixed and dispersed in the liquid (10) contained in the container (1), and the magnetic particles (11) are separated from the outside of the container (1) using magnetic means (4). By moving the container (1) to a predetermined position, the liquid (10) can be sucked at least using the suction means (2),
A liquid in which the magnetic means (4) is moved relative to the container (1) via a moving means (20), and the magnetic particles (11) are gathered at an arbitrary position in the container (1). Aggregation of magnetic particles /
In the dispersion device,
It said magnetic means (4) is permanently magnet by Rinari, wherein the magnetic means by the moving means (20) (4) so as to move the sides of the container (1),
The container (1) is made more either tube, the chip,
The moving means (20) is a motor provided on the side (22a) of the housing (22) having a number of containers (1) held in the holding holes (21) and controlled by a controller (C). and (23), a pinion (24) provided in the motor (23) meshes with the previous SL pinion (24) located vertically movably on the side portion (22a) side of the housing (22) racks (25) , a rack coupling body (27) having the rack (25), a C-shaped sliding body (28) provided in the rack coupling body (27) and having a C-shape as viewed from the longitudinal direction, A guide body (29) provided on the side portion (22a) of the housing (22) and engaged with the C-shaped sliding body (28), and connected to the rack (25) via a connection member (25a) A holding body (30) having the magnetic means (4) at the tip,
The holding body (30) is arranged in parallel in the same direction as the longitudinal direction of the rack (25) and penetrates the through hole (31) of the housing (22) into the housing (22). Protruding,
The permanent magnet of the magnetic means (4) is a set / dispersing apparatus of the magnetic particles in a liquid, characterized in Rukoto such a cylindrical or square pillars.

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