JP5330144B2 - Trace solid sample analyzer - Google Patents

Description

  The present invention relates to an instrument used for processing a small amount of a solid sample with a liquid and a method of processing a solid sample using the instrument.

  As a method for solvent extraction of a wide range of polar components from a solid sample, a multistage extraction method such as the Bligh & Dyer method is known (see Non-Patent Document 1). However, when the sample is obtained from a living body, the amount thereof is often very small, and it is difficult to take out only the extraction solvent separately from the sample using the Bligh & Dyer method.

  Therefore, as a technique related to solvent extraction of a small amount of a solid sample, Patent Document 1 proposes an extraction / separation apparatus that separates a solvent-soluble component and a solvent-insoluble component contained in the solid sample by solvent extraction. Has been. This apparatus is (a) a liquid feed pipe for supplying a solvent along a liquid feed path, (b) an extraction unit having a filter that is detachably held, and (c) an extract flowing out from the extraction unit. And (2) a holding member that integrally holds the liquid feeding pipe, the extraction unit, and the concentration unit.

  However, the extraction / separation apparatus has a structure in which the solvent for extraction flows down in the liquid supply path, so that the contact time between the solvent and the sample is short. As a result, it is difficult to extract a component with low extraction efficiency or a component present inside the sample. In addition, this apparatus is not easy to heat and extract due to its structure, and it is difficult to increase the extraction efficiency.

JP-A-2005-214785

E. G. Bligh and W.W. J. et al. Dyer, Can. J. et al. Biochem. Physiol. , 37, 911 (1957)

  The present invention provides a trace solid sample processing instrument that can eliminate the drawbacks of the above-described prior art and a method for processing a trace solid sample using the same.

  The present invention provides a trace solid sample processing instrument including a holder having a capillary part capable of holding a trace amount of solid sample therein and a holder capable of hermetically containing the holder.

Further, the present invention provides a trace solid sample in the capillary portion of the holder in the trace solid sample processing instrument,
The holder on which the sample is placed is housed in the container, and the container is filled with a treatment liquid before, after, or simultaneously with the accommodation, and then the container is sealed, and the treatment liquid is used. The present invention provides a method for treating a small amount of solid sample for treating the sample.

  According to the present invention, a small amount of a solid sample can be brought into contact with a treatment liquid for a long time. In addition, the solid sample and the processing liquid can be easily separated after the contact. Furthermore, it becomes possible to perform a heating process, and the processing efficiency by a process liquid can be improved.

Fig.1 (a) is a perspective view which shows one Embodiment of the trace amount solid sample processing instrument of this invention, FIG.1 (b) is a disassembled perspective view of the trace amount solid sample treatment instrument shown to Fig.1 (a). is there. 2A to 2D are process diagrams showing a sample processing method using the trace solid sample processing apparatus shown in FIG. It is a measurement result of cholesterol in the Example using a liquid chromatograph-mass spectrometer. It is a measurement result of a protein hydrolyzed amino acid in an Example using an amino acid analyzer.

  The present invention will be described below based on preferred embodiments with reference to the drawings. As shown in FIGS. 1A and 1B, a trace solid sample processing instrument 10 according to an embodiment of the present invention is roughly composed of a sample holder 20 and a container 30.

The sample holder 20 includes a thin tubular capillary part 21 and a cylindrical part 22 connected to the upper end of the capillary part 21. The capillary part 21 is a part capable of holding a small amount of sample therein. The capillary portion 21 has a tubular shape whose upper and lower ends are open, and the upper end is connected to the tubular portion 22 as described above. The capillary portion 21 is a fine tube having a cross-sectional area of preferably 0.1 to ³ mm ² , more preferably 0.5 to 1 mm ² . By being such a fine tube, the capillary portion 21 can successfully hold a small amount of sample. The cross-sectional area of the capillary portion 21 may be the same or different over the entire length of the capillary portion 21 on the condition that the value is within the above range. In general, the cross-sectional area is the same over the entire length of the capillary portion 21.

  The shape of the cross section of the capillary part 21 is not particularly limited. The cross-sectional shape can be generally circular. In this case, the shape of the cross section may be the same or different over the entire length of the capillary portion 21. In general, the shape of the cross section is the same over the entire length of the capillary portion 21.

  Unlike the cross-sectional area, the length of the capillary portion 21 is not particularly critical in the present invention. A satisfactory effect can be obtained by setting this length to 2 to 4 cm in general.

  The capillary part 21 has a liquid permeable filter 23 therein. The filter 23 is for placing a solid sample (not shown) thereon. Further, it is for preventing the solid sample from flowing down from the capillary part 21 together with the processing liquid. The filter 23 only needs to be liquid permeable, and examples thereof include a cotton-like body made of a fiber material, an open-cell porous body, and the like. When the filter 23 is a cotton-like body made of a fiber material, appropriate values are selected for the thickness of the fiber material and the distance between the fibers according to the amount and form of the solid sample. The same applies to the pore diameter of the porous body. These values are not critical in the present invention, and can be appropriately set by those skilled in the art.

  As a material constituting the filter 23, an appropriate material is selected according to the type of the solid sample and the type of the processing liquid. For example, various thermoplastic resins such as polyethylene, polypropylene, and acrylic resin, and silicon oxide materials such as glass and quartz can be used. Of these materials, from the viewpoint of reducing contamination, it is preferable to use a silicon oxide material that is an ashable material.

The cylindrical portion 22 is a tubular shape whose upper and lower ends are open, and the lower end thereof is smoothly connected to the upper end of the cylindrical portion 22. The tubular portion 22 has a larger cross-sectional area than the cross-sectional area of the capillary portion 21. By providing such a cylindrical portion 22, an operation of filling the capillary portion 21 with a solid sample can be easily performed. That is, while it is not easy to directly fill the capillary portion 21 having a small cross-sectional area with the solid sample, a cylindrical portion 22 having a large cross-sectional area is provided to be connected to the capillary portion 21, and the cylindrical portion is provided. By putting the solid sample in 22, it becomes easy to fill the capillary portion 21 with the solid sample. What is necessary is just to determine the cross-sectional area of the cylindrical part 22, and the shape of a cross section from this viewpoint. In general, the cross-sectional area of the cylindrical portion 22 is preferably set to ²⁰ to 30 mm ^2, and the shape of the cross section of the cylindrical portion 22 may be circular. Similarly, the length of the cylindrical portion 22 is not particularly limited as long as the solid sample can be easily put into the cylindrical portion 22. For example, the length of the cylindrical portion 22 can be set to about 5 to 7 cm.

  In the holder 20, the capillary part 21 and the cylindrical part 22 may be formed integrally and may be made of the same material, or the capillary part 21 and the cylindrical part 22 may be made of different materials. However, they may be integrated by a joining means such as adhesion or fitting.

  Regardless of whether the capillary portion 21 and the cylindrical portion 22 are made of the same material or different materials, the material constituting them may be a solid sample type or a treatment liquid type. Appropriate ones are selected according to For example, various thermoplastic resins including polyethylene, polypropylene, acrylic resin and the like, and silicon oxide materials such as glass can be used. Of these materials, from the viewpoint of reducing contamination, it is preferable to use a silicon oxide material that is an ashable material. The capillary portion 21 made of these thermoplastic resin or silicon oxide material can be obtained, for example, by heating and stretching a tubular body made of these materials.

  Next, the container 30 in which the holder 20 is accommodated will be described. The container 30 includes a container 31 and a cap 32. The accommodating part 31 is a bottomed tubular thing which has the opening part 31a in the upper end. The depth of the accommodating part 31 is larger than the full length of the holder 20. Moreover, there is no restriction | limiting in particular in the area and shape of the cross section of the accommodating part 31, and the holder 20 should just be able to be inserted in the accommodating part 31. FIG.

  The accommodating portion 31 has a portion 32 near the opening having the same thickness. On the other hand, the accommodating portion 31 is reduced in diameter as the portion 33 near the lower end thereof goes downward. Since the cross-sectional shape of the storage portion 31 is as described above, the holder 20 can be easily stored in the storage portion 31, and the holder 20 stored in the storage portion 31 is included in the storage portion 31. Makes it difficult to move. Moreover, it can prevent that the usage-amount of a process liquid (not shown) increases excessively. From these viewpoints, it is preferable that the length of the portion 33 having a reduced diameter in the accommodating portion 31 (the length in the accommodating portion 31) does not exceed the length of the capillary portion 21 in the holder 20.

  A screw thread is provided on the opening 31a of the accommodating portion 31 and the outer surface in the vicinity thereof. With this screw thread, the accommodating portion 31 and the cap 32 are screwed together. When both are screwed together, the cap 32 tightly plugs the opening 31a of the accommodating portion 31, and the accommodating device 30 can hermetically accommodate the holder 20 therein. Note that the sealing means of the cap 32 is not limited to screwing, and other means such as fitting may be adopted as long as the housing portion 31 and the cap 32 are detachable. Moreover, as long as it can be sealed, the accommodating part 31 and the cap 32 do not need to be separate bodies. For example, both may be integrated through a hinge, and the cap 32 may be fitted into the accommodating portion 31 to be in a sealed state.

  As a material constituting the container 31 in the container 30, an appropriate material is selected according to the type of the solid sample and the type of the processing liquid. As such a material, the same material as described above as the material constituting the holder 20 can be used. In particular, it is preferable to use a silicon oxide material from the viewpoint of reducing contamination. As the material constituting the cap 32 in the container 30, an appropriate material is selected according to the type of the solid sample and the type of the processing liquid. Note that the cap 32 is unlikely to be in direct contact with the treatment liquid and has a low need for ashing treatment, so the necessity of being made of a silicon oxide material is low.

  Next, a processing method using the processing tool 10 shown in FIG. 1 will be described with reference to FIGS. 2 (a) to 2 (d). First, the processing tool 10 is put into a clean state as pretreatment. When the holder 20, the filter 23, and the container 31 of the container 30 are all made of a silicon oxide material, they are ashed at a temperature of about 500 ° C. to remove organic matter, and contaminated. It is preferable to prevent this.

  When the pretreatment is completed, as shown in FIG. 2A, a small amount of the solid sample 40 is put through the opening at the upper end of the cylindrical portion 22 of the holder 20. Thereafter, the solid sample 40 is placed on the filter 23 in the capillary section 21 by lightly tapping the holder 20. The lower limit of the amount of the solid sample 40 that can be processed by this processing method is preferably 10 μg, more preferably 100 μg, although it depends on the type of the solid sample 40. There is no particular limitation on the upper limit value, but if the amount is large, the benefit of adopting this processing method is diminished. From this viewpoint, the upper limit is preferably 1 mg, more preferably 500 μg. In addition, the solid sample 40 shown to Fig.2 (a) has imaged the slice of human hair about 1 mm in length.

  The holder 20 in which the solid sample 40 is installed is accommodated in the accommodating part 31, as shown in FIG.2 (b). Before or after the accommodation or simultaneously with the accommodation, the accommodating portion 31 is filled with the processing liquid. When the treatment liquid is filled after the holder 20 is accommodated in the accommodating portion 31, the treatment liquid is injected into the holder 20 as shown in FIG. It is preferable from the viewpoint of processing. The filling amount of the processing liquid is set such that the liquid level of the filled processing liquid is higher than the position of the solid sample 40.

  When the storage of the holder 20 in the storage part 31 and the filling of the processing liquid into the storage part 31 are completed, the opening 31a at the upper end of the storage part 31 is closed with a cap 32, as shown in FIG. The inside of the accommodating part 31 is sealed. This completes the processing state. According to this processing method, unlike the technique described in Patent Document 1 described in the section of the background art, the processing liquid is held in the storage unit 31, so that the processing liquid and the solid sample 40 are in contact with each other for a long time. It becomes possible. As a result, efficient processing is possible. For example, when the processing liquid is a solvent extraction liquid, even when the solid sample 40 contains a component with low extraction efficiency or when a component existing inside the solid sample 40 is extracted, The target component can be extracted efficiently. Further, when the processing liquid is a staining liquid, the interior of the solid sample 40 can be efficiently stained. Further, when the treatment liquid is a liquid that chemically modifies the solid sample 40, the reaction can be efficiently caused to the inside of the solid sample 40.

  The treatment of the solid sample 40 with the treatment liquid may be performed in a state where the container 30 is left still, or may be performed in a state where the container 30 is gently shaken.

  As shown in FIG.2 (c), since the solid sample 40 hold | maintained at the holder 20 is sealed in the container 30 with the process liquid, it heats the container 30 by, for example, bathing in water. Can do. Thereby, it is possible to further increase the efficiency of the treatment with the treatment liquid. The degree of heating is appropriately selected according to the degree of hermeticity of the container 30, the type of the solid sample 40, the type of treatment liquid, and the like. For example, when the solid sample 40 is human hair and an organic solvent is used as the treatment liquid, the solid sample 40 can be heated to about 40 ° C., or when acid hydrolysis is performed, to about 110 ° C.

  When the solid sample 40 and the processing liquid are brought into contact with each other for a predetermined time, the cap 32 is turned to open the container 30, and the holder 20 held in the container 30 is removed as shown in FIG. Take out. At this time, a gas such as air is blown from the upper end opening of the holder 20 and the solvent remaining in the holder 20 is blown into the container 30, whereby the solvent can be efficiently recovered. In this case, since the solid sample 40 held in the holder 20 is placed on the filter 23, the solid sample 40 is taken out of the container 30 along with the removal of the holder 20. In addition, the processing liquid present in the holder 20 is drained by the filter 23 and remains in the storage unit 31. Therefore, if the processing instrument 10 of this embodiment is used, even if the amount of the solid sample 40 is very small, it can be easily and reliably separated from the processing liquid. As a result, only the treated liquid after treatment can be easily recovered. The treatment liquid is subjected to a process such as analysis, which is a subsequent process.

  Depending on the type of the solid sample 40 and the type of the processing liquid, it is advantageous to perform a multistage process. When performing multi-stage processing, as shown in FIG. 2D, after the solid sample 40 is taken out of the container 30 together with the holder 20, the treatment liquid separated from the solid sample 40 is taken out and separated. Transfer to container 50. Next, the holder 20 taken out first is accommodated in the container 30 together with the solid sample 40 in the container 30 that has been emptied. That is, the process returns to the process shown in FIG. Then, before or after housing the holder 20 in the container 30 or simultaneously with housing, the second processing liquid different from the previously used processing liquid is contained in the container 31 in the container 31 of the container 30. To fill. Thereafter, the opening 31a at the upper end of the storage unit 31 is closed with the cap 32, the interior of the storage unit 31 is sealed as shown in FIG. 2C, and the solid sample 40 is processed with the second processing liquid. Thereafter, the operation shown in FIG. 2D is performed again.

  The multistage process is not limited to twice, and may be performed three or more times as necessary. That is, the procedure shown in FIGS. 2B to 2D can be performed a plurality of times.

  There is no limitation on the type of solid sample that is the subject of this treatment method. This treatment method is particularly advantageous when a solid sample with a small amount of sample is used as the object of treatment. An example of such a solid sample is a sample collected from a living body. Specific examples include biological samples collected from human hair, human skin, and human organs. Another solid sample with a small amount collected is a small amount of fiber left on the crime scene and other solid deposits.

  On the other hand, as the treatment liquid, an appropriate one is selected according to the type of the solid sample. In view of the purpose of this treatment method, the treatment liquid should not dissolve the solid sample. Specific treatment liquids include various liquids for solvent extraction (aqueous liquid, organic solvent), staining liquid, liquid that chemically reacts with a solid sample, and bonds a predetermined group to the solid sample.

  When a solution for solvent extraction is used as the treatment liquid, multistage extraction can be performed as the above-described multistage treatment. In this case, the object A is extracted using the extraction solvent A, and then the object B is extracted using the extraction solvent B having a polarity different from that of the extraction solvent A. An extraction operation can be performed to extract an object according to the extraction solvent. According to this processing method, it is possible to perform multi-stage extraction on a small amount of solid sample without any loss or contamination.

  When a very small amount of human hair is used as the solid sample, there are the following advantages in addition to the advantages described above. Protein mass can be easily measured by hydrolyzing hair with acid after multi-stage extraction and analyzing the resulting amino acid. An amino acid quantification technique has been established ("Neochemistry Experiment Course 1, Protein II Primary Structure", Tokyo Kagaku Doujin, edited by the Japanese Biochemical Society). First, lipid is removed from the hair by solvent extraction according to the present treatment method, and then, the hair from which the lipid has been removed is completely decomposed with respect to the hair held in the holder 20 with an acid such as hydrochloric acid using the same treatment tool 10. A known amino acid is quantitatively analyzed for the liquid thus obtained. From the analysis result, the protein mass of the hair is calculated. Thereby, the ratio of the trace component contained in hair can be expressed correctly on the basis of protein mass.

  As mentioned above, although this invention was demonstrated based on the preferable embodiment, this invention is not restrict | limited to the said embodiment. For example, the holder 20 in the above embodiment is composed of the capillary part 21 and the cylindrical part 22, but the holder 20 may be composed of only the capillary part 21 instead. Moreover, although the accommodating part 31 in the accommodating tool 30 was diameter-reduced toward the downward position, the accommodating part 31 may be the same thickness over the full length instead of this.

  In the above-described embodiment, the lower end of the capillary portion 21 is in contact with the bottom inner wall of the accommodating portion 31. Instead, the upper end of the cylindrical portion 22 can be held by the cap 32, and the lower end of the capillary portion 21 is held. You may make it not contact | connect the bottom part inner wall of the accommodating part 31. FIG. By doing so, the movement of the solvent inside and outside the capillary can be further facilitated.

  Further, the shape of the lower end of the capillary portion 21 or the shape of the bottom inner wall of the accommodating portion 31 may be adjusted so that the lower end of the capillary portion 21 and a part of the bottom inner wall of the accommodating portion 31 are in contact with each other, but the other portion is not in contact. . For example, by making the cut surface of the lower end portion of the capillary portion 21 inclined, a part of the lower end portion comes into contact with the bottom inner wall of the accommodating portion 31 so that the movement of the solvent inside and outside the capillary is further facilitated. Also good.

  In addition, the processing liquid used in the above embodiment should not dissolve a solid sample when it is used for solvent extraction. However, when other processing is performed, the processing liquid that dissolves the solid sample. There is no problem with using.

  Hereinafter, the present invention will be described in more detail with reference to examples. However, the scope of the present invention is not limited to such examples.

(1) Collection of hair Six 1 cm hair portions and three 1 mm hair portions were collected from the scalp of one subject. The hair mass of each 1 cm hair was measured by a balance (the exact mass of hair cannot be measured with 1 mm hair). Three 1 cm hairs and three 1 mm hairs were accommodated in the trace solid sample processing apparatus 10 shown in FIG. On the other hand, the remaining three 1 cm hairs were accommodated in a container 30 from which the holder 20 was removed in order to examine the influence of the holder 20 on the analysis value.

(2) Extraction of hair lipids A solution of chloroform / methanol = 2/1 (volume ratio) was added to the trace solid sample processing instrument prepared above, and lipids were extracted by allowing to stand at 40 ° C. for 24 hours. After recovering the extract, similarly, chloroform / methanol = 1/1 (volume ratio) solution, chloroform / methanol = 1/2 (volume ratio) solution, chloroform / methanol / water = 18/9/1 (volume ratio). Extraction was also performed on the solution, and all the extracts were mixed. A lipid sample was prepared by drying the lipid extract prepared in this way under a nitrogen stream and dissolving it by adding methanol.

(3) Acid hydrolysis of hair 6N hydrochloric acid was added to a trace amount solid sample treatment apparatus including a hair sample remaining, and reacted at 110 ° C. for 24 hours. After all the reaction liquid was allowed to flow down and the holder 20 was removed, it was dried using an evaporator and dissolved by adding water to prepare a protein hydrolyzed amino acid sample.

(4) Measurement of cholesterol Cholesterol was measured by liquid chromatography-mass spectrometry using the lipid sample prepared in (2) above. The conditions by liquid chromatography-mass spectrometry are as follows.
(4-1) Liquid Chromatograph-Mass Spectrometer 1100 Series LC / MSD manufactured by Agilent was used as an analysis system in which a liquid chromatograph and a mass spectrometer were integrated.
(4-2) Column and separation conditions The columns and separation conditions were as follows.
Separation column: Chemical Substance Evaluation Research Organization, L-column ODS 2.1 mmΦ × 150 mm (5 μm)
Mobile phase: Methanol Mobile phase Flow rate: 0.2 mL / min Column temperature: 40 ° C
Sample solution injection volume: 5 μL
(4-3) Detection conditions in the mass spectrometer The detection conditions in the mass spectrometer were as follows.
Ionization method: APCI
Polarity: Positive ion fragmentor Voltage: 120V
Vcap voltage: 3500V
Nebulizer pressure: 60 psig
Dry gas flow rate: 4.0 L / min Dry gas temperature: 320 ° C
Vaporizer temperature: 300 ° C
Corona current: 6μA
SIM monitor ion: m / z = 369.3

(5) Measurement of protein hydrolyzed amino acid Using the protein hydrolyzed amino acid sample prepared in (3) above, amino acid was measured with an amino acid analyzer. The conditions of the amino acid analyzer are as follows.
(5-1) Amino acid analyzer L-8800 series manufactured by Hitachi, Ltd. was used as an amino acid analyzer.
(5-2) Analysis conditions The analysis conditions for protein hydrolyzed amino acids registered in the amino acid analyzer by default were used as they were.
(5-3) Abbreviations of amino acid molecules In the present specification, each amino acid molecule is represented as follows. Asp / aspartic acid, Thr / threonine, Ser / serine, Glu / glutamic acid, Pro / proline, Gly / glycine, Ala / alanine, Cys / cystine, Val / valine, Met / methionine, Ile / isoleucine, Leu / leucine, Tyr / Tyrosine, Phe / phenylalanine, Lys / lysine, His / histidine, Arg / arginine.

(6) Analysis of measurement data FIG. 3 shows measurement data of cholesterol, and FIG. 4 shows measurement data of protein hydrolyzed amino acids. For both cholesterol and amino acids, quantitative values were calculated from the peak areas, and for amino acids, the protein mass was calculated by combining the quantitative values of each amino acid molecule. Then, the absolute amount of cholesterol per hair mass or protein mass was calculated by dividing the quantitative value of cholesterol by the hair mass or protein mass.

  By the above operation, the amount of cholesterol contained in the collected hair was measured. Thereafter, a sample obtained by treating 1 cm of hair with a trace solid sample treatment instrument 10 [1], a sample obtained by treating 1 mm of hair with a trace solid sample treatment instrument 10 [2], and removing a holder 20 from a 1 cm hair. The sample processed with the instrument 30 is denoted as [3]. The absolute amount of cholesterol per hair mass in samples [1] and [3] is shown in Table 1, and the absolute amount of cholesterol per protein mass in samples [1], [2] and [3] is shown in Table 2. The quantitative values shown in the table indicate the average value and standard deviation of n = 3.

In Table 1, although there is an error between hairs, the values are not exactly the same, but almost the same quantitative values are obtained in [1] and [3], and it is proved that the trace solid sample processing instrument of the present invention is useful. It was done. On the other hand, in Table 2, since the protein mass is about 90% compared to the hair mass, the quantitative value per hair mass and the quantitative value per protein mass are slightly different, but [1], [2] And [3], almost the same quantitative values were obtained, and it was proved that the trace solid sample processing apparatus of the present invention is particularly useful for analyzing trace samples in which the mass of hair is not accurately measured.

DESCRIPTION OF SYMBOLS 10 Trace solid sample processing instrument 20 Holder 21 Capillary part 22 Cylindrical part 30 Container 31 Storage part 32 Cap 40 Trace solid sample

Claims (9)

A trace solid sample processing instrument comprising a holder having a capillary part capable of holding a trace amount of solid sample therein, and a holder capable of hermetically containing the holder ,
In the capillary part of the holder, it has a liquid permeable filter,
The container portion of the container is a trace solid sample processing instrument whose portion near the lower end is reduced in diameter as it goes downward. The trace solid sample processing instrument according to claim 1 , wherein a length of the portion having a reduced diameter in the housing portion does not exceed a length of the capillary portion in the holder . The container comprises a bottomed tubular container having an opening at the upper end, and a cap for sealing the opening;
The trace solid sample processing instrument according to claim 1 or 2, wherein the container, the holder, and the filter are made of a silicon oxide material.   The trace solid sample processing instrument according to any one of claims 1 to 3, which is used for solvent extraction processing of a solid sample. A trace solid sample is installed in the capillary part of the holder in the trace solid sample processing instrument according to claim 1,
The holder on which the sample is placed is housed in the container, and the container is filled with a treatment liquid before, after, or simultaneously with the accommodation, and then the container is sealed, and the treatment liquid is used. A method for treating a trace solid sample, wherein the sample is treated.   The processing method according to claim 5, wherein the processing liquid is a liquid for solvent extraction.   The processing method according to claim 5 or 6, wherein the solid sample is a sample collected from a living body. The sample collected from the living body is hair, and the treatment liquid is a liquid capable of extracting lipid contained in the hair,
6. The amount of protein contained in the hair is calculated by extracting lipids from the hair with the treatment liquid, hydrolyzing the hair with an acid, and quantitatively analyzing amino acids contained in the decomposition liquid. 8. The processing method according to any one of 7.   After processing the sample with the processing liquid, the container is opened to take out the processing liquid, and then the second processing liquid different from the processing liquid is filled in the container, and the container is sealed. The processing method according to claim 5, wherein the sample is processed with the second processing liquid.

Images