JP5296366B2 - Endurance improver for drugs and endurance drug - Google Patents

Description

The present invention relates to a pharmaceutical for improving endurance and Contact medicament for endurance improving agent, in particular, it relates to a pharmaceutical for enhancing endurance agent Contact and for enhancing endurance medicaments which comprise triglycerides containing medium-chain fatty acids in the constituent fatty acids as an active ingredient Is.

  Endurance is defined as the ability to maintain exercise for a certain load. Moreover, it is an independent athletic ability provided to humans, and is a basic athletic ability along with muscle strength (power), instantaneous power (speed), adjustment power (coordination), and flexibility (mobility). Furthermore, endurance is classified into several categories: the situation where the load acts (dynamic / static), the number of muscle groups (whole body / local), time (short time / long time), energy supply ( Anaerobic and aerobic), what appears as performance (power and speed) and its specific contents are diverse.

  A number of endurance assessments have been devised and implemented because of the different abilities required for each event. It can be broadly divided into evaluations by obtaining energy supply indicators and evaluations that measure performance.

  In general, examples of the energy supply index include a maximum oxygen intake amount and an anaerobic workability threshold. A method using a treadmill or a bicycle ergometer is generally used as a method for measuring the maximum oxygen intake or anaerobic threshold, and gradually increasing (stepless or multistep) loading, heart rate, expiration It is carried out by measuring gas and blood lactic acid levels.

  In addition, as an evaluation to measure performance, there are indicators that use time such as duration when given load is given and arrival time when given work, and power that is given when given time And other athletic skills such as speed and speed.

  Improving endurance is important for improving competitiveness as an element of improving athletic ability, although the contents differ depending on the competition as described above.

  In order to improve endurance, daily physical training occupies an important position, but it has recently been recognized that diet in daily life is important for improving endurance obtained as a result of training.

  Therefore, various studies have been made on active ingredients for improving endurance by eating (see, for example, Patent Document 1 and Patent Document 2).

  On the other hand, medium-chain fatty acid triglycerides have high oxidative stability, low freezing point and viscosity, and are almost colorless and transparent, and have high solubility. It has been used for pharmaceutical raw materials. Moreover, since it has good absorbability and a higher energy ratio than saccharides, it is also used as a raw material for enteral nutrients for the purpose of efficient energy supplementation.

  As a food composition containing a medium chain fatty acid triglyceride, for example, in Patent Document 3, a food composition containing a medium chain fatty acid triglyceride and a saccharide can contribute to storage of glycogen in the liver and muscle, It is described that it is useful for nutritional supplementation, fatigue recovery, stamina enhancement and the like.

However, it has not been clarified that the endurance improving function has a function of improving endurance by taking medium-chain fatty acid triglycerides into a normal diet without difficulty.
JP 2007-254313 A JP 2005-272437 A Japanese Patent Publication No. 8-29055

  As described above, there is a need for an active ingredient for improving endurance by eating, and in particular, it can be easily consumed and can improve endurance, and Foods that can be used without concern for side effects are desired.

Accordingly, an object of the present invention can be easily ingested, and is to provide a pharmaceutical for enhancing endurance agent Contact and enhancing endurance drugs that can be used without side effects concerns.

  As a result of intensive studies to solve the above-mentioned problems, the present inventors have an excellent endurance-improving effect of triglycerides containing medium-chain fatty acids in constituent fatty acids (hereinafter referred to as medium-chain fatty acid triglycerides). And since it can be ingested easily in daily life, it has been found useful as a food or a drug for improving endurance, and the present invention has been completed.

In order to achieve the above object, the present invention contains a medium-chain fatty acid triglyceride as an active ingredient in an amount of 5% by mass or more, and 95% by mass or more of the constituent fatty acids in the medium-chain fatty acid triglyceride is a saturated fatty acid having 8 and 10 carbon atoms ( Caprylic acid and capric acid), the mass ratio is caprylic acid / capric acid = 70/30 to 80/20, and the proportion of caprylic acid in the total fatty acids bonded to the 1,3-positions of triglycerides is 65 Provided is a pharmaceutical endurance improver that is ˜80 mass%.

Moreover, this invention provides the pharmaceutical for endurance improvement characterized by including the endurance improving agent for pharmaceuticals of the said invention in order to achieve the said objective.

  The endurance improving action referred to in the present invention means that the duration of exercise when a certain amount of load is given by its ingestion (particularly continuous ingestion) is extended, and that the performance of exercise is improved / improved. This can also be called an action (anti-fatigue action) that makes it difficult to get tired during exercise, and is different from the action that recovers fatigue caused by exercise or the like as described in Patent Document 3 described above.

According to the present invention, it can be easily ingested, and, it is possible to provide a pharmaceutical for the endurance enhancer you and for enhancing endurance drugs that can be used without the side effects of concern.

[Endurance improver according to the embodiment of the present invention]
The endurance improver according to the embodiment of the present invention contains medium-chain fatty acid triglyceride as an active ingredient.

(Composition of medium chain fatty acid triglyceride)
The medium chain fatty acid triglyceride in the present invention is a triglyceride containing a medium chain fatty acid as a constituent fatty acid, that is, triacylglycerol.

  Here, the medium chain fatty acid is preferably a fatty acid having 6 to 12 carbon atoms, particularly a saturated fatty acid. Among these, caproic acid, caprylic acid, capric acid, and lauric acid, which are saturated fatty acids having an even number of carbon atoms, are more preferable, and caprylic acid and capric acid, which are saturated fatty acids having 8 to 10 carbon atoms, are particularly preferable.

  90% by mass or more (more preferably 95 to 100% by mass) of the constituent fatty acids of the medium chain fatty acid triglyceride is a saturated fatty acid (caprylic acid and capric acid) having 8 and 10 carbon atoms, and the mass ratio thereof is caprylic acid / caprin. Acid = 60/40 to 85/15 (more preferably 70/30 to 80/20), and the proportion of caprylic acid in the total fatty acids bonded to the 1,3-positions of triglycerides is 60 to 85% by mass ( More preferably, it is preferably 65 to 80% by mass.

(Method for producing medium chain fatty acid triglyceride)
Although the manufacturing method of such a medium chain fatty acid triglyceride is not specifically limited, For example, it can obtain by performing an ester bond reaction by using as a raw material the medium chain fatty acid and glycerol derived from palm kernel oil or coconut oil.

  The conditions of the ester bond reaction are not particularly limited, but can be obtained by, for example, reacting with no catalyst and no solvent while removing water produced as a by-product under normal pressure or reduced pressure. Of course, such a medium-chain fatty acid triglyceride can also be obtained by a reaction using a catalyst such as acid or alkali, an enzyme or a solvent.

  By mixing tricaprylic acid glyceride and capric acid with a special mixing ratio and using a lipase having positional specificity, for example, “Lipase PL” manufactured by Meisho Sangyo Co., Ltd., 90% by mass or more of the constituent fatty acids have 8 and 10 carbon atoms. Of saturated fatty acids (caprylic acid and capric acid), the mass ratio of which is caprylic acid / capric acid = 60/40 to 85/15, and capryl in all fatty acids bonded to the 1,3-positions of triglycerides A medium chain fatty acid triglyceride having an acid ratio of 60 to 85% by mass can be obtained.

  It is also possible to obtain medium chain fatty acid triglycerides per se from oil seeds of genetically modified plants, or to produce medium chain fatty acid triglycerides using medium chain fatty acids obtained from oil seeds of genetically modified plants. is there.

(Composition of endurance improver)
The endurance improver according to the embodiment of the present invention preferably contains 5% by mass (˜100% by mass) of the medium chain fatty acid triglyceride as an active ingredient. 30-85 mass% is more preferable, and 48-80 mass% is further more preferable.

  The endurance improver according to the embodiment of the present invention can contain components other than medium-chain fatty acid triglycerides.

  Examples of such ingredients include, but are not limited to, normal edible oils and fats such as soybean oil, rapeseed oil, high oleic rapeseed oil, corn oil, sesame oil, sesame salad oil, perilla oil, linseed oil, peanut oil, safflower oil, high olein Safflower oil, sunflower oil, high oleic sunflower oil, cottonseed oil, grape seed oil, macadamia nut oil, hazelnut oil, pumpkin seed oil, walnut oil, camellia oil, tea seed oil, sesame oil, borage oil, olive oil, rice oil, rice bran Oil, wheat germ oil, palm oil, palm kernel oil, palm oil, cocoa butter, beef tallow, lard, chicken fat, milk fat, fish oil, seal oil, algae oil, these oils and fats that have been desaturated by breeding Examples thereof include mixed fats and oils, hydrogenated fats and oils, and fractionated fats and oils.

  Also, vitamin A, vitamin E, plant sterol, polyglycerol fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, ascorbic acid fatty acid ester, organic acid monoglyceride, lignan, coenzyme Q, amino acid, methylsulfonylmethane, glucosamine, chondroitin Ascorbic acid stearate, ascorbyl palmitate, phospholipid, oryzanol, diglyceride and the like can also be added.

  Vitamin A refers to retinol, which is abundant in animal foods such as meat and fish, and provitamin A (α-carotene, β-, which is abundant in plant foods such as green-yellow vegetables and fruits that change to retinol in the body. Generic name of carotene, cryptoxanthin, etc.), and may also be synthesized. Vitamin E is a generic name for multiple types of tocopherols and tocotrienols that are abundant in plants and cereals, and is classified into four types of α, β, γ, and δ, respectively, and is synthesized by synthesis. It may be the one given.

  Among these, it is preferable to contain at least one selected from edible fats and vitamins A. When edible fats and oils are contained, it is preferably 60% by mass or less, more preferably 5 to 55% by mass, and particularly preferably 5 to 50% by mass with respect to the total endurance improver. Moreover, when it contains vitamin A, it is preferable that it is 0.00001-0.01 mass% of the whole endurance improving agent, More preferably, it is 0.00001-0.003 mass%.

  As said edible fats and oils, the transesterified edible fats and oils obtained by transesterifying the edible fats and oils which mixed 2 or more types of the said edible fats and oils can also be used, for example. The transesterification includes a chemical reaction using an alkali catalyst and an enzyme reaction using an enzyme, and the enzyme reaction is preferable in terms of flavor and color of edible fats and oils, but is not limited thereto. The composition of the fatty acid at the 1st and 3rd positions and the fatty acid at the 2nd position of the obtained transesterified edible oil may or may not be biased.

(Form of endurance improver)
Although the form of the endurance improving agent according to the embodiment of the present invention is not particularly limited, it can be used in all forms of oral administration preparations such as capsules, tablets, and powders.

(Dose of endurance improver)
Although the dosage of the endurance improving agent according to the embodiment of the present invention is not particularly limited, it should be administered in an amount of 6 g / day or more in terms of the amount of medium-chain fatty acid triglyceride as an active ingredient per adult. Is preferred.

(Food for improving endurance and pharmaceuticals for improving endurance)
The endurance improver according to the embodiment of the present invention may be added to foods (processed foods, seasonings, confectionery, etc.) and pharmaceuticals, for example, gelatin capsules, edible oils and fats, dressings, butters, margarines, preparations Margarine, fat spread, cream, ice cream, mayonnaise, shortening, bakery mix, bread, pie, cake, cookie, donut, muffin, scone, fried food, snack confectionery, chocolate, topping, processed meat products, frozen food, fried food, It can be added to enteral nutrients, parenteral nutrients, and liquid foods.

  In addition, with the permission of the Ministry of Health, Labor and Welfare, it can be used as a food for specified health use that displays effects (uses) related to functions (actions) based on improving endurance and anti-fatigue. In other words, food for endurance improvement that contains medium chain fatty acid triglycerides as an active ingredient for improving endurance and displays the effect (use) related to the function (action) based on the endurance improvement according to the permission of the Ministry of Health, Labor and Welfare as the effect (use) (Excluding beverages).

  The content of medium-chain fatty acid triglycerides in the composition excluding moisture in the food for improving endurance is preferably 3% by mass or more, and more preferably 5% by mass or more.

  The endurance improving agent according to the embodiment of the present invention, and the endurance improving food and the endurance improving pharmaceutical containing the same are not limited to humans, but animals other than humans, such as horses, dogs, cows, chickens. Can be used for.

[Effect of the embodiment of the present invention]
According to the embodiment of the present invention, it is possible to provide an endurance improver that is highly effective, safe and easy to implement, and foods and pharmaceuticals containing the endurance enhancer. Endurance can be improved by taking these endurance improvers, endurance foods and pharmaceuticals on a daily basis, so it is possible to improve the athletic ability of athletes, exercise enthusiasts and recreational implementers, Moreover, the improvement effect of the competition ability of these subjects can be expected.

EXAMPLES Next, although an Example demonstrates this invention, this invention is not limited by these Examples. Examples 2 to 4 are reference examples.

  Medium chain fatty acid triglyceride (trade name: O.D.O) manufactured by Nisshin Oilio Group Co., Ltd. was used as endurance improver 1.

  500 g of rapeseed oil made by Nisshin Oilio Group was mixed with 500 g of medium chain fatty acid triglyceride (trade name: OD.O) made by Nisshin Oilio Group, and 1 kg of endurance improver 2 was obtained. .

  100 g of lard made by Snow Brand Milk Products Co., Ltd. was mixed with 900 g of medium chain fatty acid triglyceride (trade name: OD.O) manufactured by Nisshin Oilio Group, and 1 kg of endurance improver 3 was obtained.

  Add 1 g of tocopherol (trade name: Tocopherol 85) made by Nisshin Oilio Group to 999 g of medium chain fatty acid triglyceride (trade name: OD) made by Nisshin Oilio Group Co., Ltd. After stirring and dissolving, 1 kg of endurance improver 4 was obtained.

  After mixing 750g of medium chain fatty acid triglyceride (trade name: O.D.O) made by Nisshin Oilio Group, 250g of starch / dextrin mixture and 1500g of water, the mixture was emulsified with an emulsifier. It was spray-dried to 5% or less, and about 1 kg of endurance improver 5 was obtained.

  Among the constituent fatty acids of medium chain fatty acid triglyceride (trade name: OD.O) manufactured by Nisshin Oilio Group, 99.9% by mass is a saturated fatty acid having 8 and 10 carbon atoms, and 8 carbon atoms. The mass ratio of saturated fatty acids of 10 and 10 was 7.4: 2.6. Moreover, in the composition of the fatty acid bonded to the 1,3-position of the triglyceride, the ratio of the saturated fatty acid having 8 carbon atoms was 73% by mass. The ratio of fatty acid bonded to the 2-position of triglyceride is calculated according to the Brockerhoff method (Brockerhoff, H., “Stereospecific analysis of triglycerides: an alternative method.”, Journal of Lipid Research (USA), 1967, Vol. 8, Vol. 3, p.167-169).

  Using the endurance improver 1 of Example 1, fat spread (food for endurance improvement 1) was prepared by the following blending ratio and preparation method. In addition, this fat spread (food 1 for endurance improvement) contains 5g of the endurance improver 1 per serving (14g).

[Mixing ratio (parts by mass)]
Edible refined processed oil: 30.8 parts 30% β-carotene: 10 ppm
Emulsifier: 0.5 part Water: 27.2 parts Salt: 1.1 parts Milk powder: 1 part Fragrance: 0.1 part Endurance improver 1: 39.3 parts

[Preparation method]
The endurance improver 1 was heated and mixed together with edible refined processed oil and fat, 30% β-carotene, emulsifier, and fragrance, and then preliminarily emulsified with water, salt and powdered milk, and then rapidly kneaded and kneaded with a combinator.

  Using the fat spread of Example 2 (endurance improving food 1), a nutritionally adjusted food (endurance improving food 2) was prepared by the following blending ratio and preparation method. The endurance improving food 2 contains 6 g of endurance improving agent 1 per meal (100 g).

[Mixing ratio (parts by mass)]
Flour: 33.3 parts Sugar: 29.9 parts Endurance food: 16.6 parts Processed starch: 8.3 parts Egg: 3.3 parts Dietary fiber: 2.3 parts Nonfat dry milk: 1.6 parts Nutritional enhancer (vitamin B1, B2, B6, mineral): 1.2 parts Emulsifier: 0.1 part Fragrance: 0.1 part Water: 3.3 parts

[Preparation method]
Sugar, food for improving endurance 1, egg, dietary fiber, skim milk powder, nutrient fortifier and emulsifier were mixed, and flour, processed starch and flavor were added and mixed. Then, water was added, kneaded, rolled and molded, heated and fired.

(Endurance improvement test)
The endurance improvement effect test by the crossover method was carried out using the endurance improvement food 2 of Example 3. As a control food, the same blending ratio and preparation method as in Examples 2 and 3 except that endurance improver 1 was replaced with the same amount of general-purpose salad oil (rapeseed oil: soybean oil = 70:30 (mass ratio) blended oil). The nutritionally adjusted food prepared by the above was used.

  The test was conducted on 8 healthy adults (1 male, 7 females) (crossover method) who have been conducting physical activity once a week for 30 minutes or longer for 3 months or longer. Before the start of the test, a gradually increasing load was applied with a bicycle ergometer to measure the maximum oxygen intake of each person, and the maximum power (load) obtained at that time was determined. The endurance improving food 2 or the control food was continuously ingested for 13 days until the day before the test. After fasting for 12 hours from the day before the test, the endurance food 2 or the control food was ingested in the morning of the test.

  After 1 hour of eating, ride on a bicycle ergometer and let warm-up for 5 minutes, then exercise for 40 minutes with a load equivalent to 60% of the maximum oxygen intake, and then continue with a load equivalent to 80% of the maximum oxygen intake The exercise was continued until exhaustion, and the exercise duration was measured when exercised with a load equivalent to 80% of the maximum oxygen intake. The test results are shown in FIG.

  Furthermore, at the start of warm-up exercise, blood sampling and subjective exercise intensity (PRE; rating of perceived) 15 minutes and 30 minutes after the start of exercise with a load equivalent to 60%, and immediately after the start of exercise with a load equivalent to 80%. Measurement of exertion, Borg index). From the collected blood, blood glucose level and blood lactic acid level were measured with a portable blood glucose / lactic acid measuring device. The test results are shown in Table 1.

  As shown in FIG. 1, the exercise duration in the exercise given a load equivalent to 80% of the maximum oxygen intake is compared with the control food by ingesting the endurance improving food 2 of the present invention. Significantly prolonged. The * mark shown in FIG. 1 shows a statistically significant difference in the average value when the endurance improving food 2 containing the endurance improving agent of the present invention is ingested compared to that of the control food. (Risk rate is 5% or less).

  Similarly, as shown in Table 1, the blood lactate level and the subjective exercise intensity during exercise are increased after 20 minutes of exercise during exercise by ingesting the endurance improving food 2 of the present invention. It was significantly suppressed compared with the control food.

  From these facts, it was revealed that the endurance improving food 2 containing the endurance improving agent of the present invention has significantly improved endurance by continuous intake.

It is a graph which shows the endurance improvement effect test result of Example 4.

Claims (4)

Containing 5% by mass or more of triglyceride containing medium chain fatty acid in constituent fatty acid (hereinafter referred to as medium chain fatty acid triglyceride) as an active ingredient,
95 mass% or more of the constituent fatty acids of the medium chain fatty acid triglyceride is a saturated fatty acid having 8 and 10 carbon atoms (caprylic acid and capric acid), and the mass ratio thereof is caprylic acid / capric acid = 70/30 to 80/20. The endurance improver for pharmaceuticals in which the ratio of caprylic acid in the total fatty acids bonded to positions 1 and 3 of triglycerides is 65 to 80% by mass. Drug for endurance improver according to claim 1, characterized by further containing edible fats and oils. Furthermore, vitamin A is contained, The endurance improver for pharmaceuticals of Claim 1 or Claim 2 characterized by the above-mentioned. A pharmaceutical for endurance improvement comprising the endurance improver for pharmaceuticals according to any one of claims 1 to 3 .