JP5182782B2 - Composition for preventing fatigue accumulation - Google Patents
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- JP5182782B2 JP5182782B2 JP2007013905A JP2007013905A JP5182782B2 JP 5182782 B2 JP5182782 B2 JP 5182782B2 JP 2007013905 A JP2007013905 A JP 2007013905A JP 2007013905 A JP2007013905 A JP 2007013905A JP 5182782 B2 JP5182782 B2 JP 5182782B2
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Description
本発明は、豆乳の乳酸菌発酵物由来の疲労蓄積予防組成物に関する。 The present invention relates to a fatigue accumulation preventing composition derived from a fermented lactic acid bacterium of soy milk.
運動は、新陳代謝を促進するとともに心肺機能の向上など健康状態の維持には欠かせない。自発運動は、強制的ではなく自らの意思によって引き起こされる運動を意味する。 Exercise is essential for maintaining a healthy state, such as promoting metabolism and improving cardiopulmonary function. Spontaneous movement means movement caused by one's intention, not forced.
生体が疲労状態にあると、自発運動量が減少する。その一方で、疲労回復がなされることで、自発運動量が上昇する。これらのことから、自発運動量は疲労の蓄積の指標として考えられている。 When the living body is in a fatigued state, the spontaneous momentum decreases. On the other hand, spontaneous recovery increases as fatigue recovery is performed. From these facts, spontaneous momentum is considered as an index of fatigue accumulation.
疲労蓄積の予防は、日常の運動量が増加し、生体の運動機能の向上及び維持に寄与するため、優れた疲労蓄積予防組成物は、医薬品あるいは機能性飲食品として有用である。 Since prevention of fatigue accumulation increases the amount of daily exercise and contributes to improvement and maintenance of the motor function of the living body, an excellent fatigue accumulation prevention composition is useful as a pharmaceutical or a functional food or drink.
自発運動は、チオールプロテアーゼ及び/又はセリンプロテアーゼによりタンパク質を分解して得られるペプチドやアミノ酸によって促進されることが従来知られている。これらのペプチドやアミノ酸は、朝鮮人参、ローヤルゼリーやプロポリスプロテインなどの機能性飲食品と同様の疲労回復効果によって自発運動を促進することが知られている（例えば、特許文献１〜２参照）。しかし、前述の機能性飲食品は、高価であり日常の食生活での多用が困難であり、より安価に摂取することができる機能性飲食品が望まれている。 It is conventionally known that the spontaneous movement is promoted by a peptide or amino acid obtained by degrading a protein with a thiol protease and / or a serine protease. These peptides and amino acids are known to promote spontaneous exercise by the same fatigue recovery effect as functional foods and drinks such as ginseng, royal jelly, and propolis protein (see, for example, Patent Documents 1 and 2). However, the functional foods and drinks described above are expensive and difficult to be frequently used in daily eating habits, and functional foods and drinks that can be consumed at a lower cost are desired.
本発明は、新規な乳酸菌豆乳発酵物由来の疲労蓄積予防組成物の提供を課題とする。 An object of the present invention is to provide a novel composition for preventing fatigue accumulation derived from a fermented soymilk of lactic acid bacteria.
本発明は以下に関する。
（１）豆乳の乳酸菌発酵物を有効成分とする、疲労蓄積予防組成物。
（２）乳酸菌が、Leuconostoc属に属する微生物である、上記（１）記載の疲労蓄積予防組成物。
（３）乳酸菌が、Leuconostoc pseudomesenteroides ATCC 12291株である、上記（１）記載の疲労蓄積予防組成物。
（４）豆乳に乳酸菌を添加して発酵を行わせた後、発酵物を採取することを特徴とする、上記（１）から（３）記載の疲労蓄積予防組成物の製造法。
（５）乳酸菌が、Leuconostoc属に属する微生物である、上記（４）記載の疲労蓄積予防組成物の製造法。
（６）乳酸菌が、Leuconostoc pseudomesenteroides ATCC 12291株である、上記（４）記載の疲労蓄積予防組成物の製造法。
The present invention relates to the following.
(1) A composition for preventing fatigue accumulation, comprising fermented lactic acid bacteria of soy milk as an active ingredient.
(2) The fatigue accumulation preventing composition according to (1) above, wherein the lactic acid bacteria are microorganisms belonging to the genus Leuconostoc.
(3) The fatigue accumulation preventing composition according to the above (1), wherein the lactic acid bacterium is Leuconostoc pseudomesenteroides ATCC 12291 strain.
(4) The method for producing a fatigue accumulation-preventing composition according to (1) to (3) above, wherein the fermented material is collected after adding lactic acid bacteria to soy milk to cause fermentation.
(5) The method for producing a fatigue accumulation preventing composition according to (4) above, wherein the lactic acid bacteria are microorganisms belonging to the genus Leuconostoc.
(6) The method for producing a fatigue accumulation preventing composition according to the above (4), wherein the lactic acid bacterium is Leuconostoc pseudomesenteroides ATCC 12291 strain.
１．疲労蓄積予防組成物とその製造法
本発明の疲労蓄積予防組成物は、豆乳の乳酸菌発酵物を有効成分とする。該発酵物は、豆乳に乳酸菌を添加して発酵を行わせた後、発酵物を採取することによって得られる。
1. Fatigue accumulation preventing composition and method for producing the same The fatigue accumulation preventing composition of the present invention comprises a fermented lactic acid bacterium of soy milk as an active ingredient. The fermented product can be obtained by collecting a fermented product after adding lactic acid bacteria to soy milk to cause fermentation.
本発明でいう疲労蓄積予防活性とは、疲労の蓄積を予防し、自発運動量若しくは自発意欲を増加させる活性を意味する。本発明のように、豆乳の乳酸菌発酵物を有効成分とする疲労蓄積予防組成物を摂取することにより、疲労の蓄積が予防され、自発運動促進効果、疲労回復効果、ストレス予防効果、スタミナ持続効果等に寄与する。 The fatigue accumulation preventing activity referred to in the present invention means an activity for preventing the accumulation of fatigue and increasing the amount of spontaneous exercise or volition. As in the present invention, by ingesting a fatigue accumulation preventing composition comprising a fermented lactic acid bacterium of soy milk as an active ingredient, accumulation of fatigue is prevented, spontaneous exercise promoting effect, fatigue recovery effect, stress preventing effect, stamina sustaining effect Contribute to etc.
また、単に豆乳を含有させた食品に比べ、豆乳の乳酸菌発酵物の摂取により優れた疲労蓄積予防効果を発揮し、単なる豆乳由来のタンパク質やアミノ酸の摂取による効果ではなく、豆乳の乳酸菌発酵物の摂取により初めて効果が発揮される。 In addition, compared to foods that simply contain soy milk, the intake of fermented lactic acid bacteria in soy milk has an excellent effect of preventing fatigue accumulation, not the effects of mere intake of soy milk-derived proteins and amino acids, The effect is demonstrated only by ingestion.
本発明でいう豆乳とは、水に浸漬して膨潤させた大豆を摩砕した液体、又は該液体からおからを分離して得られる液体をいう。原料となる大豆の品種は特に限定されず、例えば「りゅうほう」「たちゆたか」等が使用できる。また、各種市販豆乳若しくは公知技術を用いて製造した豆乳も使用することも可能である。 The term “soy milk” as used in the present invention refers to a liquid obtained by grinding soybean soaked and swollen in water, or a liquid obtained by separating okara from the liquid. The variety of soybean used as a raw material is not particularly limited. For example, “Ryuho”, “Tachiyutaka” and the like can be used. In addition, various commercially available soymilks or soymilks produced using known techniques can also be used.
疲労蓄積予防活性を有する発酵物が得られる限り、本発明で使用する乳酸菌の種類は特に限定されない。例えば、Leuconostoc属に属する乳酸菌が使用できる。より具体的には、Leuconostoc mesenteroides、Leuconostoc paramesenteroides、Leuconostoc pseudomesenteroides、Leuconostoc citreum、Leuconostoc dextranicumなどが挙げられる。さらに具体的には、Leuconostoc pseudomesenteroides (ATCC12291)、Leuconostoc citreum (NRIC1579)、Leuconostoc paramesenteroides (NISL7218)が挙げられる。 The type of lactic acid bacteria used in the present invention is not particularly limited as long as a fermented product having fatigue accumulation preventing activity is obtained. For example, lactic acid bacteria belonging to the genus Leuconostoc can be used. More specifically, Leuconostoc mesenteroides, Leuconostoc paramesenteroides, Leuconostoc pseudomesenteroides, Leuconostoc citreum, Leuconostoc dextranicum and the like can be mentioned. More specifically, Leuconostoc pseudomesenteroides (ATCC12291), Leuconostoc citreum (NRIC1579), Leuconostoc paramesenteroides (NISL7218) can be mentioned.
なお、上記菌種名に関し、「ＮＲＩＣ」とは東京農業大学応用生物科学部菌株保存室が保存する菌株を意味し、「ＮＩＳＬ」とは、財団法人野田産業科学研究所が保存する菌株を意味する。「ＡＴＣＣ」は、米国ＶＡ２０１１０−２２０９、マナサス、ユイバーシティブルーバード１０８０１のＡＴＣＣにあるアメリカンタイプカルチャーコレクション国際寄託当局を指す。 In addition, regarding the above bacterial species names, “NRIC” means a strain preserved by the Tokyo University of Agriculture, Department of Applied Biological Sciences, and the “NISL” means a strain preserved by the Noda Institute of Industrial Science. To do. “ATCC” refers to the American Type Culture Collection International Depositary Authority at ATCC in USA VA20110-2209, Manassas, Uyber City Bluebird 10801.
豆乳に乳酸菌を添加して発酵を行わせる方法は特に限定されず、例えば、培養した乳酸菌の菌液を豆乳に添加した後、用いた乳酸菌に適した温度、時間等の条件、例えば、２５〜３５℃で４時間以上の発酵条件を適宜設定して発酵を行えばよい。乳酸菌が嫌気性菌の場合は嫌気条件にて発酵を行う。なお、発酵は、複数種の乳酸菌を使用した混合発酵又は連続発酵であってもよい。発酵性を向上させるために、必要な栄養源、例えば、シュークロース、マルトース、スタキオース又はラフィノースから選択される１種以上の糖類などを豆乳に添加してもよい。 The method of adding lactic acid bacteria to soymilk and causing fermentation is not particularly limited. For example, after adding the bacterial solution of cultured lactic acid bacteria to soymilk, conditions such as temperature and time suitable for the lactic acid bacteria used, for example, 25 to 25 What is necessary is just to perform fermentation by setting suitably the fermentation conditions for 4 hours or more at 35 degreeC. When lactic acid bacteria are anaerobic bacteria, fermentation is performed under anaerobic conditions. Note that the fermentation may be mixed fermentation or continuous fermentation using a plurality of types of lactic acid bacteria. In order to improve fermentability, a necessary nutrient source, for example, one or more sugars selected from sucrose, maltose, stachyose or raffinose may be added to soy milk.
疲労蓄積予防活性を発揮する限り、上記で得られた発酵物をそのまま本発明の疲労蓄積予防組成物として使用してもよい。また、該発酵物を更に分画処理して、より強い疲労蓄積予防活性を有する画分を使用してもよい。分画は、例えば、溶媒抽出法、ゲル濾過カラム、限外濾過膜等を用いて行うことができる。 As long as the fatigue accumulation preventing activity is exhibited, the fermented product obtained above may be used as it is as the fatigue accumulation preventing composition of the present invention. Further, the fermented product may be further fractionated to use a fraction having a stronger fatigue accumulation preventing activity. Fractionation can be performed using, for example, a solvent extraction method, a gel filtration column, an ultrafiltration membrane, or the like.
本発明の疲労蓄積予防組成物は、医薬品、飲食品又は飲食品用素材として使用することができる。医薬品、飲食品又は飲食品用素材の種類、形態、及びその他の含有成分等には特に制約はなく、当業者に公知の任意の各種方法で容易に加工することができる。疲労蓄積予防組成物を使用する場合の有効成分の摂取量は、摂取者の年令、体重、適応症状などによって異なる。例えば、体重６０ｋｇの成人による経口摂取の場合、１日１回又は数回摂取し、その摂取量は１日当たり１回約１００ｇ〜２００ｇ、好ましくは１．６〜３．５ｇ／ｋｇ体重程度とすればよい。この疲労蓄積予防組成物は、継続摂取用とすることにより極めて優れた効果を発揮する。 The fatigue accumulation preventing composition of the present invention can be used as a pharmaceutical, a food or drink, or a food or drink material. There is no restriction | limiting in particular in the kind of medicine, food / beverage products, or the raw material for food / beverage products, and another containing component, It can process easily by arbitrary various methods well-known to those skilled in the art. The intake of the active ingredient when using the fatigue accumulation preventing composition varies depending on the age, weight, indication symptoms, etc. of the intaker. For example, in the case of oral intake by an adult weighing 60 kg, take once or several times a day, and the intake is about 100 g to 200 g, preferably about 1.6 to 3.5 g / kg body weight once a day. That's fine. This fatigue accumulation preventing composition exhibits an extremely excellent effect when used for continuous intake.
２．本発明の疲労蓄積予防組成物
本発明の疲労蓄積予防組成物は、豆乳の乳酸菌発酵物を含有し、かつ、疲労蓄積予防活性を有する。
2. Fatigue accumulation preventing composition of the present invention The fatigue accumulation preventing composition of the present invention contains a fermented lactic acid bacterium of soy milk and has fatigue accumulation preventing activity.
疲労蓄積予防活性を有する限り、豆乳の乳酸菌発酵物をそのまま機能性飲食品として使用してもよいし、該発酵物を他の食品、例えば、肉製品、水産加工品、加工野菜、加工果実、惣菜類、大豆加工品、食用粉類、食用蛋白質、飲料、酒類、調味料、乳製品、菓子に添加して使用してもよい。飲食品としての形状は、特に限定されず、固形状、乳状、ペースト状、半固形状、液状とすればよい。 Soy milk lactic acid bacteria fermented product may be used as a functional food or drink as it is, as long as it has fatigue accumulation preventing activity, and the fermented product may be used as another food, for example, meat products, processed fishery products, processed vegetables, processed fruits, It may be used by adding it to sugar beet, processed soybean products, edible powders, edible proteins, beverages, alcoholic beverages, seasonings, dairy products, and confectionery. The shape of the food or drink is not particularly limited, and may be solid, milky, pasty, semisolid, or liquid.
必要により、例えば、以下に示す原材料、トマト、にんじん、タマネギ、にんにく、ゴマなどの野菜の加工処理物（ピューレ、ペースト、練状物、細断粒状物、磨砕物、粉末など）；リンゴ、ユズ、オレンジ、モモ、イチゴ、パイナップル、ミカン、ブドウなどの果実、果汁の加工処理物（同上）などを適宜添加混和してもよい。 If necessary, for example, raw materials shown below, processed products of vegetables such as tomatoes, carrots, onions, garlic, sesame (purees, pastes, kneaded products, shredded granular materials, ground products, powders, etc.); apples, yuzu Fruits such as oranges, peaches, strawberries, pineapples, mandarin oranges and grapes, processed products of fruit juice (same as above), etc. may be added and mixed as appropriate.
本発明の機能性飲食品は、疲労蓄積予防活性を通じて自発運動量を増加させ、運動機能の向上維持、筋肉量の増加、心肺機能の向上、肥満予防、疲労回復効果、滋養強壮効果、痴呆予防効果等を発揮することが可能である。なお、本発明の疲労蓄積予防組成物は、ヒト用のみならずペットフードや家畜用飼料として使用することができる。 The functional food and drink of the present invention increases the amount of spontaneous exercise through fatigue accumulation preventing activity, improves and maintains motor function, increases muscle mass, improves cardiopulmonary function, obesity prevention, fatigue recovery effect, nutrition tonic effect, dementia prevention effect Etc. can be exhibited. The fatigue accumulation preventing composition of the present invention can be used not only for humans but also as pet food and livestock feed.
以下により、豆乳の乳酸菌発酵物を調製し、その疲労蓄積予防活性を確認した。 By the following, fermented lactic acid bacteria of soy milk were prepared, and their fatigue accumulation preventing activity was confirmed.
〔回転ケージでの測定〕
豆乳の乳酸菌発酵物は、次のようにして作製した。豆腐用豆乳（紀文フードケミファ社製）を５０％、２０％ Ｓｕｃｒｏｓｅ（１２１℃、１５分滅菌済み）を５０％含有する豆乳培地に、乳酸菌を１０６−１０７ｃｆｕ／ｍｌになるように接種し、３０℃で２４時間培養した。使用した乳酸菌は、Leuconostoc pseudomesenteroides ATCC 12291株である。
[Measurement with rotating cage]
The lactic acid bacteria fermented product of soy milk was prepared as follows. Inoculate soy milk for tofu (manufactured by Kibun Food Chemifa Co., Ltd.) and 50% 20% sucrose (sterilized at 121 ° C. for 15 minutes) with lactic acid bacteria to 10 6 -10 7 cfu / ml And cultured at 30 ° C. for 24 hours. The lactic acid bacterium used is Leuconostoc pseudomesenteroides ATCC 12291 strain.
５週齢のＦｉｓｃｈｅｒ３４４系雄性ラットを回転ケージ内で飼育した。試験開始から３週間を普通飼料としてＭＦ飼料を自由摂取させ、２４時間あたりのケージ回転数を記録した。その後、豆乳の乳酸菌発酵物を５％の割合で添加した豆乳の乳酸菌発酵物添加飼料（本発明）、又は豆乳（比較例）を５％の割合で添加した豆乳添加飼料を３週間にわたって自由摂取させて、同様にケージ回転数を記録した。その後、さらにＭＦ飼料を３週間にわたって自由摂取させてケージ回転数を記録した。 Five week old Fischer 344 male rats were housed in a rotating cage. The MF feed was freely ingested as a normal feed for 3 weeks from the start of the test, and the number of cage rotations per 24 hours was recorded. After that, the soymilk lactic acid bacteria fermented product supplemented with the soymilk lactic acid bacteria fermented product added at a rate of 5% (the present invention), or the soymilk supplemented feed added with the soymilk (comparative example) at the rate of 5% can be freely ingested for 3 weeks. The cage rotation speed was recorded in the same manner. Thereafter, the MF diet was further ad libitum over 3 weeks, and the cage rotation speed was recorded.
試験結果を図１に示す。普通飼料から豆乳の乳酸菌発酵物添加飼料（本発明）に変更することによって、有意に回転ケージの回転数が増加した。その後、普通飼料に再度戻すことによってケージの回転数は有意に減少をした。一方で、普通飼料から豆乳添加飼料（比較例）に変更しても回転ケージの回転数には変化が認められなかった。自発運動量は、図１に示すとおり、豆乳の乳酸菌発酵物添加飼料群（本発明）において、豆乳添加飼料群（比較例）に対して約５０％の大幅な自発運動増加が見られ、疲労蓄積予防に豆乳の乳酸菌発酵物（本発明）が大きく寄与していることが示された。このことによって、豆乳の乳酸菌発酵物が、単なる豆乳由来のタンパク質やアミノ酸の摂取による効果ではなく、豆乳の乳酸菌発酵物の摂取により初めて効果が発揮されることが示された。 The test results are shown in FIG. By changing from normal feed to soy milk lactic acid bacteria fermented product-added feed (invention), the rotational speed of the rotating cage increased significantly. Thereafter, the number of cage rotations was significantly reduced by returning to normal diet. On the other hand, even if it changed from normal feed to soymilk addition feed (comparative example), the change in the rotation speed of the rotation cage was not recognized. As shown in FIG. 1, the amount of spontaneous exercise showed a significant increase in spontaneous exercise of about 50% in the soymilk-added feed group (invention), compared to the soymilk-added feed group (invention), and accumulated fatigue. It was shown that the fermented lactic acid bacteria of the soy milk (the present invention) greatly contributed to prevention. Thus, it was shown that the fermented lactic acid bacteria of soymilk was not effective by the intake of mere proteins or amino acids derived from soymilk, but the effect was exhibited for the first time by the intake of fermented lactic acid bacteria of soymilk.
本発明の疲労蓄積予防組成物は、医薬品、飲食品又は飲食品用素材として使用することができる。該疲労蓄積予防組成物は、任意の飲食品に添加して使用できるので、疲労蓄積予防を通じて、自発運動量促進効果、運動機能の向上維持効果、筋肉量の増加効果、心肺機能の向上、肥満予防効果、疲労回復効果、滋養強壮効果、痴呆予防効果を発揮する機能性食品を容易に得ることができる。 The fatigue accumulation preventing composition of the present invention can be used as a pharmaceutical, a food or drink, or a food or drink material. Since the fatigue accumulation preventing composition can be used by adding it to any food or drink, through the prevention of fatigue accumulation, the effect of promoting spontaneous exercise amount, the effect of maintaining and improving motor function, the effect of increasing muscle mass, the improvement of cardiopulmonary function, the prevention of obesity A functional food that exhibits effects, fatigue recovery effects, nourishing tonic effects, and dementia prevention effects can be easily obtained.
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